488 results match your criteria Archivio italiano di urologia andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica[Journal]


Transcriptome Profiles of Human Visceral Adipocytes in Obesity and Colorectal Cancer Unravel the Effects of Body Mass Index and Polyunsaturated Fatty Acids on Genes and Biological Processes Related to Tumorigenesis.

Front Immunol 2019 19;10:265. Epub 2019 Feb 19.

Center for Gender-Specific Medicine, Istituto Superiore di Sanità, Rome, Italy.

Obesity, a low-grade inflammatory condition, represents a major risk factor for the development of several pathologies including colorectal cancer (CRC). Although the adipose tissue inflammatory state is now recognized as a key player in obesity-associated morbidities, the underlying biological processes are complex and not yet precisely defined. To this end, we analyzed transcriptome profiles of human visceral adipocytes from lean and obese subjects affected or not by CRC by RNA sequencing ( = 6 subjects/category), and validated selected modulated genes by real-time qPCR. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389660PMC
February 2019
1 Read

Artificial Neural Network Prediction of Retention of Amino Acids in Reversed-Phase HPLC under Application of Linear Organic Modifier Gradients and/or pH Gradients.

Molecules 2019 Feb 11;24(3). Epub 2019 Feb 11.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67100 Coppito, L'Aquila, Italy.

A multi-layer artificial neural network (ANN) was used to model the retention behavior of 16 -phthalaldehyde derivatives of amino acids in reversed-phase liquid chromatography under application of various gradient elution modes. The retention data, taken from literature, were collected in acetonitrile⁻water eluents under application of linear organic modifier gradients ( gradients), pH gradients, or double pH/ gradients. At first, retention data collected in  gradients and pH gradients were modeled separately, while these were successively combined in one dataset and fitted simultaneously. Read More

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http://www.mdpi.com/1420-3049/24/3/632
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http://dx.doi.org/10.3390/molecules24030632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384946PMC
February 2019
6 Reads

Geographical discrimination of red garlic (Allium sativum L.) produced in Italy by means of multivariate statistical analysis of ICP-OES data.

Food Chem 2019 Mar 20;275:333-338. Epub 2018 Sep 20.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

Sixty-five samples of red garlic (Allium sativum L.) coming from four different production territories of Italy were analysed by means of inductively coupled plasma optical emission spectrometry. The garlic samples were discriminated according to the geographical origin using the content of seven elements (Ba, Ca, Fe, Mg, Mn, Na and Sr). Read More

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http://dx.doi.org/10.1016/j.foodchem.2018.09.088DOI Listing
March 2019
1 Read

The Structure of a Conserved Telomeric Region Associated with Variant Antigen Loci in the Blood Parasite Trypanosoma congolense.

Genome Biol Evol 2018 09 1;10(9):2458-2473. Epub 2018 Sep 1.

Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, United Kingdom.

African trypanosomiasis is a vector-borne disease of humans and livestock caused by African trypanosomes (Trypanosoma spp.). Survival in the vertebrate bloodstream depends on antigenic variation of Variant Surface Glycoproteins (VSGs) coating the parasite surface. Read More

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https://academic.oup.com/gbe/article/10/9/2458/5079403
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http://dx.doi.org/10.1093/gbe/evy186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152948PMC
September 2018
8 Reads

UHPLC Analysis of Saffron ( L.): Optimization of Separation Using Chemometrics and Detection of Minor Crocetin Esters.

Molecules 2018 Jul 25;23(8). Epub 2018 Jul 25.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67100 L'Aquila, Italy.

Ultra-high performance liquid chromatography (UHPLC) coupled with diode array detection (DAD) was applied to improve separation and detection of mono- and bis-glucosyl esters of crocetin (crocins), the main red-colored constituents of saffron ( L.), and other polar components. Response surface methodology (RSM) was used to optimise the chromatographic resolution on the Kinetex C18 (Phenomenex) column taking into account of the combined effect of the column temperature, the eluent flow rate and the slope of a linear eluent concentration gradient. Read More

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http://dx.doi.org/10.3390/molecules23081851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222919PMC
July 2018
4 Reads

[Mobile miniature X-ray evaluation and pneumoconiosis: the role of the Clinica del Lavoro in Milan (1941-1948)].

Med Lav 2018 05 28;109(3):225-35. Epub 2018 May 28.

Università degli Studi di Milano.

Since the end of the 19th century, X-rays have been used to detect lung diseases. In Italy, 207,096 miniature chest radiographs were taken from 1941 to 1948. Traditional radiographs gave better results, but miniature chest radiographs were useful for screening. Read More

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http://dx.doi.org/10.23749/mdl.v109i3.7164DOI Listing
May 2018
2 Reads

Analysis of intraspecific seed diversity in Astragalus aquilanus (Fabaceae), an endemic species of Central Apennine.

Plant Biol (Stuttg) 2019 May 6;21(3):507-514. Epub 2018 Jun 6.

Majella Seed Bank, Majella National Park, Loc. Colle Madonna, Lama dei Peligni (CH), Italy.

This work aims to study seeds of the endemic species Astragalus aquilanus from four different populations of central Italy. We investigated seed morpho-colorimetric features (shape and size) and chemical differences (through infrared spectroscopy) among populations and between dark and light seeds. Seed morpho-colorimetric quantitative variables, describing shape, size and colour traits, were measured using image analysis techniques. Read More

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http://dx.doi.org/10.1111/plb.12844DOI Listing
May 2019
2 Reads

Effect of protocatechuic acid on insulin responsiveness and inflammation in visceral adipose tissue from obese individuals: possible role for PTP1B.

Int J Obes (Lond) 2018 Dec 16;42(12):2012-2021. Epub 2018 May 16.

Center for Gender-Specific Medicine, Italian National Institute of Health, Viale Regina Elena 299, Rome, 00161, Italy.

Background/objectives: The occurrence of chronic inflammation in visceral adipose tissue (VAT) in obese subjects precipitates the development of insulin resistance and type 2 diabetes (T2D). Anthocyanins and their main metabolite protocatechuic acid (PCA) have been demonstrated to stimulate insulin signaling in human adipocytes. The aim of this study was to investigate whether PCA is able to modulate insulin responsiveness and inflammation in VAT from obese (OB) and normal weight (NW) subjects. Read More

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http://dx.doi.org/10.1038/s41366-018-0075-4DOI Listing
December 2018
11 Reads

Joint Data Analysis in Nutritional Epidemiology: Identification of Observational Studies and Minimal Requirements.

J Nutr 2018 02;148(2):285-297

Molecular Epidemiology Research Group, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Background: Joint data analysis from multiple nutrition studies may improve the ability to answer complex questions regarding the role of nutritional status and diet in health and disease.

Objective: The objective was to identify nutritional observational studies from partners participating in the European Nutritional Phenotype Assessment and Data Sharing Initiative (ENPADASI) Consortium, as well as minimal requirements for joint data analysis.

Methods: A predefined template containing information on study design, exposure measurements (dietary intake, alcohol and tobacco consumption, physical activity, sedentary behavior, anthropometric measures, and sociodemographic and health status), main health-related outcomes, and laboratory measurements (traditional and omics biomarkers) was developed and circulated to those European research groups participating in the ENPADASI under the strategic research area of "diet-related chronic diseases. Read More

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http://dx.doi.org/10.1093/jn/nxx037DOI Listing
February 2018
20 Reads

Of mice and men: Traces of life in the death registries of the 1630 plague in Milano.

J Proteomics 2018 05 3;180:128-137. Epub 2018 Jan 3.

Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, Milano 20131, Italy. Electronic address:

The death registries of the plague epidemic of 1630, stored at the Archivio di Stato of Milano, have been interrogated via the EVA film technology (ethyl vinyl acetate film studded with crushed strong anion and cation exchangers as well as C resins). The EVA diskettes have been left in contact with the lower right margins of 11 different pages pertaining to the peak months of the raging disease (June through end of September) for 60-90min and then the captured material, after elution and digestion, analysed by mass spectrometry. The main findings: 17 Yersiniaceae family proteins, 31 different human keratins, 22 unique mouse keratins, about 400 peptides from different bacterial strains, 58 human tissue proteins and 130 additional mouse and rat tissue proteins. Read More

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http://dx.doi.org/10.1016/j.jprot.2017.11.028DOI Listing
May 2018
16 Reads

Adsorption of triazine herbicides from aqueous solution by functionalized multiwall carbon nanotubes grown on silicon substrate.

Nanotechnology 2018 Feb;29(6):065701

Department of Physical and Chemical Science, University of L'Aquila, via Vetoio, I-67100, Coppito, L'Aquila, Italy.

Multi-walled carbon nanotubes (MWCNTs), because of their small size and large available surface area, are potentially efficient sorbents for the extraction of water solutes. Dispersion of MWCNTs in aqueous medium is suitable to adsorb organic contaminants from small sample volumes, but, the recovery of the suspended sorbent for successive re-use represents a critical step, which makes this method inapplicable in large-scale water-treatment technologies. To overcome this problem, we proposed here MWCNTs grown on silicon supports and investigated on a small-volume scale their adsorption properties towards triazine herbicides dissolved in water. Read More

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http://dx.doi.org/10.1088/1361-6528/aaa0a0DOI Listing
February 2018
1 Read

Recent Evidence on the Role of Dietary PUFAs in Cancer Development and Prevention.

Curr Med Chem 2018 ;25(16):1818-1836

Center for Gender-specific Medicine, Gender-specific Prevention and Health Unit, Istituto Superiore di Sanita, Rome, Italy.

Background: Scientific evidence has been accumulated about the effects of polyunsaturated fatty acids (PUFAs) on human health. The hypothesis that n-3 PUFAs might improve the efficiency of anticancer drugs has recently been considered. The role of n-6 PUFAs, in contrast, needs to be better assessed. Read More

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http://www.eurekaselect.com/158052/article
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http://dx.doi.org/10.2174/0929867325666171204160231DOI Listing
February 2019
7 Reads

Anti-inflammatory Activity of Extra Virgin Olive Oil Polyphenols: Which Role in the Prevention and Treatment of Immune-Mediated Inflammatory Diseases?

Endocr Metab Immune Disord Drug Targets 2018 ;18(1):36-50

Istituto Superiore di Sanita, Center for Gender-specific Medicine, Viale Regina Elena 299; 00161 Rome, Italy.

Background And Objective: Altered inflammatory response characterizes chronic immunemediated inflammatory diseases (IMID) such as rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, systemic lupus erythematosus, and psoriasis. Accumulating evidence indicates that regular consumption of extra virgin olive oil (EVOO), the main source of fat in the Mediterranean diet, is associated with a reduced risk of developing chronic degenerative disorders such as cardiovascular diseases, type 2 diabetes and cancer. The beneficial effects on health of EVOO have been attributed, besides to the monounsaturated fats content, to the presence of phenolic compounds that have antioxidant, anti-inflammatory and immunomodulatory properties. Read More

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http://dx.doi.org/10.2174/1871530317666171114114321DOI Listing
September 2018
10 Reads

Extraction of curcuminoids by using ethyl lactate and its optimisation by response surface methodology.

J Pharm Biomed Anal 2018 Feb 1;149:89-95. Epub 2017 Nov 1.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67100 Coppito, L'Aquila, Italy.

Response surface methodology (RSM) was applied to optimise the extraction of curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) from turmeric using ethyl lactate (EL), ethanol and water under mild conditions (magnetic stirring at room temperature). An augmented simplex-centroid mixture design was used to monitor the dependence of the extraction efficiency from the proportions of the three solvents in the extraction medium. HPLC was used to establish the content of curcuminoids in turmeric and in the extracts. Read More

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http://dx.doi.org/10.1016/j.jpba.2017.10.042DOI Listing
February 2018
3 Reads

Genetic susceptibility and periodontal disease: a retrospective study on a large italian sample.

Oral Implantol (Rome) 2017 Jan-Mar;10(1):20-27. Epub 2017 Apr 10.

Department of Medicine and Surgery, University of Insubria, Varese, Italy.

Background: Periodontal disease (PD) is a multifactorial illness in which environment and host interact. The genetic component plays a key role in the onset of PD. In fact the genetic compound can modulate the inflammation of the mucous membranes and the loss of alveolar bone. Read More

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http://dx.doi.org/10.11138/orl/2017.10.1.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516423PMC

Distinct Blood and Visceral Adipose Tissue Regulatory T Cell and Innate Lymphocyte Profiles Characterize Obesity and Colorectal Cancer.

Front Immunol 2017 9;8:643. Epub 2017 Jun 9.

Center for Gender-Specific Medicine, Istituto Superiore di Sanità, Rome, Italy.

Visceral adipose tissue (VAT) is a main site where metabolic and immunologic processes interplay to regulate, at local and systemic level, the inflammatory status and immune response. Obesity-associated inflammation and immune dysfunctions are inextricably linked to tumor but, in spite of intense efforts, the mechanisms underpinning this association remain elusive. In this report, we characterized the profile of VAT-associated and circulating innate lymphocyte and regulatory T (T) cell subsets underlying inflammatory conditions, such as obesity and colorectal cancer (CRC). Read More

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http://dx.doi.org/10.3389/fimmu.2017.00643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465245PMC
June 2017
24 Reads

Hospitals of Rome in the Early Nineteenth Century: The Apostolic Visit of 1825.

Authors:
Jacalyn Duffin

Can Bull Med Hist 2016 7;33(2):517-553. Epub 2016 Sep 7.

Hannah Chair of the History of Medicine, Queen's University.

Pope Leo XII marked the 1825 Jubilee by visiting the hospitals of Rome. Italy was recovering from the French invasion that had disrupted social and religious structures. The Visitors investigated conditions, and recommended changes. Read More

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http://dx.doi.org/10.3138/cbmh.33.2.163-26062015DOI Listing
December 2017

Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes.

J Cell Biol 2017 02 29;216(2):379-391. Epub 2016 Dec 29.

School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, England, UK

Kinetochores are multiprotein complexes that couple eukaryotic chromosomes to the mitotic spindle to ensure proper segregation. The model for kinetochore assembly is conserved between humans and yeast, and homologues of several components are widely distributed in eukaryotes, but key components are absent in some lineages. The recent discovery in a lineage of protozoa called kinetoplastids of unconventional kinetochores with no apparent homology to model organisms suggests that more than one system for eukaryotic chromosome segregation may exist. Read More

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http://dx.doi.org/10.1083/jcb.201608043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294786PMC
February 2017

Investigation by response surface methodology of the combined effect of pH and composition of water-methanol mixtures on the stability of curcuminoids.

Food Chem 2017 Mar 28;219:414-418. Epub 2016 Sep 28.

Hortus Novus, Via Collepietro, 67100 L'Aquila, Italy. Electronic address:

Response surface methodology, coupled to a full factorial three-level experimental design, was applied to investigate the combined influence of pH (between 7.0 and 8.6) and composition of methanol-water mixtures (between 30 and 70% v/v of methanol content) on the stability of curcumin and its analogues demethoxycurcumin and bisdemethoxycurcumin. Read More

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http://dx.doi.org/10.1016/j.foodchem.2016.09.167DOI Listing
March 2017
1 Read

Geographical identification of saffron (Crocus sativus L.) by linear discriminant analysis applied to the UV-visible spectra of aqueous extracts.

Food Chem 2017 Mar 28;219:408-413. Epub 2016 Sep 28.

Hortus Novus, Via Collepietro, 67100 L'Aquila, Italy. Electronic address:

We attempted geographical classification of saffron using UV-visible spectroscopy, conventionally adopted for quality grading according to the ISO Normative 3632. We investigated 81 saffron samples produced in L'Aquila, Città della Pieve, Cascia, and Sardinia (Italy) and commercial products purchased in various supermarkets. Exploratory principal component analysis applied to the UV-vis spectra of saffron aqueous extracts revealed a clear differentiation of the samples belonging to different quality categories, but a poor separation according to the geographical origin of the spices. Read More

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http://dx.doi.org/10.1016/j.foodchem.2016.09.169DOI Listing

Consumption of extra-virgin olive oil rich in phenolic compounds improves metabolic control in patients with type 2 diabetes mellitus: a possible involvement of reduced levels of circulating visfatin.

J Endocrinol Invest 2016 Nov 25;39(11):1295-1301. Epub 2016 Jun 25.

Unit of Nutrition, Department of Veterinary Public Health and Food Safety, Italian National Institute of Health, Viale Regina Elena 299, 00161, Rome, Italy.

Aim: Phenolic compounds naturally contained in extra-virgin olive oil (EVOO) have demonstrated anti-inflammatory and antioxidant properties. The present study aimed at evaluating the effects of a polyphenol-rich extra-virgin olive oil (EVOO) (high-polyphenol EVOO, HP-EVOO) on the metabolic control and the production of specific pro-/anti-inflammatory adipokines in overweight patients with type 2 diabetes mellitus (T2D).

Methods: Eleven overweight T2D patients not in treatment with insulin were invited to follow their habitual diet for a total of 8 weeks. Read More

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http://dx.doi.org/10.1007/s40618-016-0506-9DOI Listing
November 2016
33 Reads

Jung's views of Nazi Germany: the first year and Jung's transition.

J Anal Psychol 2016 Sep;61(4):481-96

East Lansing, MI, USA.

This article first considers Jung's response to the coming to power of the Nazis in Germany. It brings forth evidence that, besides wanting to preserve psychotherapy in Germany and maintain the international connection between the German and other communities of psychotherapists, he wanted to advance Jungian psychology - his psychology - in Germany. It also presents evidence that, although he occasionally made some anti-Semitic statements during this early period, he was not anti-Semitic in the way the Nazis were. Read More

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http://dx.doi.org/10.1111/1468-5922.12238DOI Listing
September 2016
1 Read

Visceral fat adipocytes from obese and colorectal cancer subjects exhibit distinct secretory and ω6 polyunsaturated fatty acid profiles and deliver immunosuppressive signals to innate immunity cells.

Oncotarget 2016 Sep;7(39):63093-63105

Departments of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.

Obesity is a low-grade chronic inflammatory state representing an important risk factor for colorectal cancer (CRC). Adipocytes strongly contribute to inflammation by producing inflammatory mediators. In this study we investigated the role of human visceral fat adipocytes in regulating the functions of innate immunity cells. Read More

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http://dx.doi.org/10.18632/oncotarget.10998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325349PMC
September 2016
4 Reads

Geographical classification of Italian saffron (Crocus sativus L.) based on chemical constituents determined by high-performance liquid-chromatography and by using linear discriminant analysis.

Food Chem 2016 Dec 24;212:110-6. Epub 2016 May 24.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

One hundred and forty-four Italian saffron samples produced in the years from 2009 to 2015 in five distinct areas located in four different regions, Abruzzo (L'Aquila), Tuscany (Florence), Umbria (Cascia and Città della Pieve) and Sardinia, have been analysed by high-performance liquid chromatography with diode array detection. Intensities of the chromatographic peaks attributed to crocins, safranal, picrocrocin and its derivatives and flavonoids were considered as variables in linear discriminant analysis to attempt geographical classification. The results revealed that spices produced at different sites of the Italian territory can be discriminated with good accuracy. Read More

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http://dx.doi.org/10.1016/j.foodchem.2016.05.149DOI Listing
December 2016
37 Reads

Optimisation by response surface methodology of microextraction by packed sorbent of non steroidal anti-inflammatory drugs and ultra-high performance liquid chromatography analysis of dialyzed samples.

J Pharm Biomed Anal 2016 Jun 23;125:114-21. Epub 2016 Mar 23.

Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti, Pescara, Via dei Vestini, 66100 Chieti, Italy.

A procedure based on microextraction by packed sorbent (MEPS) followed by ultra-high performance liquid chromatography (UHPLC) with photodiode array (PDA) detection has been developed for the analysis of seven selected non steroidal anti-inflammatory drugs (NSAIDs) in human dialysates. The influence on MEPS efficiency of pH of the sample, pH of the washing solvent and methanol content in the hydro-alcoholic elution mixture has been investigated by response surface methodology based on a Box-Behnken design of experiments. Among the above factors, pH of sample is the variable that mostly influences MEPS recovery. Read More

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http://dx.doi.org/10.1016/j.jpba.2016.03.045DOI Listing

Optimisation of temperature-programmed gas chromatographic separation of organochloride pesticides by response surface methodology.

J Chromatogr A 2015 Dec 30;1423:149-57. Epub 2015 Oct 30.

Agenzia Regionale per la Tutela dell' Ambiente (ARTA) Abruzzo, Caselle di Bazzano, Bivio per Monticchio, 67100 L'Aquila, Italy.

A response surface methodology (RSM) approach is applied to optimise the temperature-programme gas-chromatographic separation of 16 organochloride pesticides, including 12 compounds identified as highly toxic chemicals by the Stockholm Convention on Persistent Organic Pollutants. A three-parameter relationship describing both linear and curve temperature programmes is derived adapting a model previously used in literature to describe concentration gradients in liquid chromatography with binary eluents. To investigate the influence of the three temperature profile descriptors (the starting temperature, the gradient duration and a shape parameter), a three-level full-factorial design of experiments is used to identify suitable combinations of the above variables spanning over a useful domain. Read More

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http://dx.doi.org/10.1016/j.chroma.2015.10.082DOI Listing
December 2015

Estimating Sampling Selection Bias in Human Genetics: A Phenomenological Approach.

PLoS One 2015 9;10(10):e0140146. Epub 2015 Oct 9.

Dipartimento di Biologia, University of Pisa, Via Ghini 13, 56126 Pisa, Italy.

This research is the first empirical attempt to calculate the various components of the hidden bias associated with the sampling strategies routinely-used in human genetics, with special reference to surname-based strategies. We reconstructed surname distributions of 26 Italian communities with different demographic features across the last six centuries (years 1447-2001). The degree of overlapping between "reference founding core" distributions and the distributions obtained from sampling the present day communities by probabilistic and selective methods was quantified under different conditions and models. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140146PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599962PMC
June 2016
5 Reads

Protocatechuic Acid Prevents oxLDL-Induced Apoptosis by Activating JNK/Nrf2 Survival Signals in Macrophages.

Oxid Med Cell Longev 2015 9;2015:351827. Epub 2015 Jun 9.

Department of Veterinary Public Health and Food Safety, Nutrition Unit, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

Protocatechuic acid (PCA), one of the main metabolites of complex polyphenols, exerts numerous biological activities including antiapoptotic, anti-inflammatory, and antiatherosclerotic effects. Oxidised LDL have atherogenic properties by damaging arterial wall cells and inducing p53-dependent apoptosis in macrophages. This study was aimed at defining the molecular mechanism responsible for the protective effects of PCA against oxidative and proapoptotic damage exerted by oxLDL in J774 A. Read More

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http://dx.doi.org/10.1155/2015/351827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477133PMC
April 2016
3 Reads

A retrospective study of clinical and radiologic outcomes of 69 consecutive maxillary sinus augmentations associated with functional endoscopic sinus surgery.

Int J Oral Maxillofac Implants 2015 May-Jun;30(3):633-8

Purpose: The aim of this clinical investigation was to evaluate the clinical and radiologic outcomes of a single-step surgical procedure that includes functional endoscopic sinus surgery (FESS) and maxillary sinus elevation by the lateral window approach in patients with reversible contraindications to sinus elevation.

Materials And Methods: Thirty-eight patients with insufficient bone height in the posterior maxilla caused by pneumatization of the sinus and with reversible ear-nose-throat (ENT) contraindications to sinus elevation were recruited for this investigation between January 2010 and January 2012. All patients were treated in a single session under general anesthesia for a total of 69 consecutive sinus augmentations. Read More

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http://dx.doi.org/10.11607/jomi.3757DOI Listing
February 2016
2 Reads

Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity.

Mol Nutr Food Res 2015 Aug 29;59(8):1472-81. Epub 2015 May 29.

Department of Veterinary Public Health and Food Safety, Italian National Institute of Health, Rome, Italy.

Scope: Insulin resistance represents an independent risk factor for metabolic and cardiovascular diseases. Researchers have been interested in identifying active harmless compounds, as many insulin-sensitizing drugs have shown unwanted side-effects. It has been demonstrated that anthocyanins and one of their representative metabolites, protocatechuic acid (PCA), ameliorate hyperglycemia, and insulin sensitivity. Read More

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http://dx.doi.org/10.1002/mnfr.201400816DOI Listing
August 2015
3 Reads

Quantitative structure-retention relationships of cannabimimetic aminoalkilindole derivatives and their metabolites.

J Pharm Biomed Anal 2015 May 25;109:136-41. Epub 2015 Feb 25.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

Development of chromatographic analyses of synthetic cannabinoids is complicated by the lack of commercial reference standards, especially for new analogues introduced in the clandestine market to bypass legal controls and for their metabolites. In the present work, we explore the possibility of predicting the retention behaviour of the cannabimimetic aminoalkilindoles and their urinary metabolites in high-performance liquid-chromatography using a quantitative structure-retention relationship (QSRR) generated by multilinear regression. To represent the structure of the 43 investigated analytes, 617 computational molecular descriptors are subjected to genetic algorithm variable selection aimed at identifying a small but informative subset. Read More

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http://dx.doi.org/10.1016/j.jpba.2015.02.036DOI Listing
May 2015
1 Read

Ancient administrative handwritten documents: X-ray analysis and imaging.

J Synchrotron Radiat 2015 Mar 30;22(2):446-51. Epub 2015 Jan 30.

Faculté des Sciences de Base, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Handwritten characters in administrative antique documents from three centuries have been detected using different synchrotron X-ray imaging techniques. Heavy elements in ancient inks, present even for everyday administrative manuscripts as shown by X-ray fluorescence spectra, produce attenuation contrast. In most cases the image quality is good enough for tomography reconstruction in view of future applications to virtual page-by-page `reading'. Read More

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http://dx.doi.org/10.1107/S1600577515000314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786057PMC

Development of molecularly imprinted polymeric nanofibers by electrospinning and applications to pesticide adsorption.

J Sep Sci 2015 May 14;38(8):1402-10. Epub 2015 Mar 14.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell' Aquila, Via Vetoio, Coppito, L'Aquila, Italy.

Novel polystyrene-based molecularly imprinted polymer nanofibers were synthesized through the electrospinning technique. The molecularly imprinted polymers were prepared using a non-covalent approach and atrazine as template. For comparison, nonimprinted polymer nanofibers were also synthesized. Read More

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http://doi.wiley.com/10.1002/jssc.201500033
Publisher Site
http://dx.doi.org/10.1002/jssc.201500033DOI Listing
May 2015
3 Reads

GRANTING A LICENCE FOR OPENING A PHARMACY IN BOLOGNA DURING ACTIVITY OF THE BOLOGNESE ARTE DE' SPEZIALI (13TH - 18TH CENTURY).

Med Secoli 2015 ;27(1):215-40

The article discusses the main changes in legislation concerning granting the licenses for opening a new pharmacy in Bologna in the Middle Ages and Early Modern period. The organization of all traders, including apothecaries, was subordinated, as almost everywhere in Italy, to the Guilds. In the 2nd half of 16th century the Arte de' Speziali of Bologna came under the jurisdiction of the Collegio di Medicina, leading to disagreements between the two corporations. Read More

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Artificial neural network prediction of multilinear gradient retention in reversed-phase HPLC: comprehensive QSRR-based models combining categorical or structural solute descriptors and gradient profile parameters.

Anal Bioanal Chem 2015 Feb 14;407(4):1181-90. Epub 2014 Nov 14.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, Coppito, 67010, L'Aquila, Italy,

A multilayer artificial neural network (ANN) is used to model the reversed-phase liquid chromatography retention times of 16 selected compounds, including purines, pyrimidines and nucleosides. The analysed data, taken from literature, were collected in acetonitrile-water eluents under the application of 16 different multilinear gradients. The parameters describing the gradient profile together with solute descriptors are considered as the independent variables of an ANN-based model providing the retention time as response. Read More

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http://dx.doi.org/10.1007/s00216-014-8317-3DOI Listing
February 2015
1 Read

Cross-column prediction of gas-chromatographic retention indices of saturated esters.

J Chromatogr A 2014 Aug 6;1355:269-77. Epub 2014 Jun 6.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67100 Coppito, L'Aquila, Italy.

We combine computational molecular descriptors and variables related with the gas-chromatographic stationary phase into a comprehensive model able to predict the retention of solutes in external columns. To explore the quality of various approaches based on alternative column descriptors, we analyse the Kováts retention indices (RIs) of 90 saturated esters collected with seven columns of different polarity (SE-30, OV-7, DC-710, OV-25, XE-60, OV-225 and Silar-5CP). Cross-column retention prediction is evaluated on an internal validation set consisting of data of 40 selected esters collected with each of the seven columns, sequentially excluded from calibration. Read More

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http://dx.doi.org/10.1016/j.chroma.2014.06.002DOI Listing

Prediction of the retention of s-triazines in reversed-phase high-performance liquid chromatography under linear gradient-elution conditions.

J Sep Sci 2014 Aug 12;37(15):1930-6. Epub 2014 Jun 12.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, L'Aquila, Italy.

In this paper, a multilayer artificial neural network is used to model simultaneously the effect of solute structure and eluent concentration profile on the retention of s-triazines in reversed-phase high-performance liquid chromatography under linear gradient elution. The retention data of 24 triazines, including common herbicides and their metabolites, are collected under 13 different elution modes, covering the following experimental domain: starting acetonitrile volume fraction ranging between 40 and 60% and gradient slope ranging between 0 and 1% acetonitrile/min. The gradient parameters together with five selected molecular descriptors, identified by quantitative structure-retention relationship modelling applied to individual separation conditions, are the network inputs. Read More

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http://dx.doi.org/10.1002/jssc.201400346DOI Listing
August 2014
1 Read

Modelling of UPLC behaviour of acylcarnitines by quantitative structure-retention relationships.

J Pharm Biomed Anal 2014 Aug 13;96:224-30. Epub 2014 Apr 13.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

In the present work, the retention time (RT) of acylcarnitines, collected by ultra-performance liquid-chromatography after formation of butyl esters, is modelled by quantitative structure-retention relationship (QSRR) method. The investigated set consists of free carnitine and 46 different acylcarnitines, including the isomers commonly monitored in screening metabolic disorders. To describe the structure of (butylated) acylcarnitines, a large number of computational molecular descriptors generated by software Dragon are subjected to variable selection methods aimed at identifying a small informative subset. Read More

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http://dx.doi.org/10.1016/j.jpba.2014.04.006DOI Listing

Analysis of the mineral composition of Italian saffron by ICP-MS and classification of geographical origin.

Food Chem 2014 Aug 27;157:485-9. Epub 2014 Feb 27.

Istituto Nazionale di Fisica Nucleare, Laboratorio Nazionale del Gran Sasso, S.S. 17/bis km 18+910, 67010 Assergi, L'Aquila, Italy.

27 Saffron spices produced in three Italian regions, Abruzzo (L'Aquila), Umbria and Sardinia, were analysed by inductively coupled plasma-mass spectrometry to test potentiality of mineral composition for geographical traceability. A linear discriminant analysis (LDA) based on concentrations of 12 selected elements, Li, B, Na, Ga, Rb, Sr, Zr, Nb, Cs, Ba, Sm, and Hf, allows more than 80% of correct predictions in leave-one cross-validation. Four elements (B, Na, Sr and Rb), identified by step-wise LDA, provide more than 90% of correct predictions. Read More

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http://dx.doi.org/10.1016/j.foodchem.2014.02.068DOI Listing

ω3-PUFAs exert anti-inflammatory activity in visceral adipocytes from colorectal cancer patients.

PLoS One 2013 7;8(10):e77432. Epub 2013 Oct 7.

Department Veterinary Public Health and Food Safety, Rome, Italy.

Objective: The aim of this study was to correlate specific fatty acid profiles of visceral white adipose tissue (WAT) with inflammatory signatures potentially associated with colorectal cancer (CRC).

Methods: Human adipocytes were isolated from biopsies of visceral WAT from 24 subjects subdivided in four groups: normal-weight (BMI 22.0-24. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077432PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792028PMC
June 2014
4 Reads
10 Citations
3.234 Impact Factor

Volatiles fingerprint of Artemisia umbelliformis subsp. eriantha by headspace-solid phase microextraction GC-MS.

Nat Prod Res 2014 20;28(1):61-6. Epub 2013 Aug 20.

a Department of Physical and Chemical Sciences , University of L'Aquila, Via Vetoio, Coppito , I-67100 , L'Aquila (AQ) , Italy.

Artemisia umbelliformis subsp. eriantha is a protected species, whose essential oil is used in liqueur industry. Volatile profiles of fresh leaves and flowers from wild plants in comparison with regenerated in vitro plants introduced in experimental fields within an Italian national park were evaluated by headspace-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS). Read More

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http://dx.doi.org/10.1080/14786419.2013.825912DOI Listing
August 2014
9 Reads

In vivo imaging of trypanosomes for a better assessment of host-parasite relationships and drug efficacy.

Parasitol Int 2014 Feb 25;63(1):260-8. Epub 2013 Jul 25.

Institut Pasteur, Laboratoire des Processus Infectieux à Trypanosomatidés, Département Infection et Epidemiologie, 25 rue du Dr. Roux, 75724 Paris, France.

The advances in microscopy combined to the invaluable progress carried by the utilization of molecular, immunological or immunochemical markers and the implementation of more powerful imaging technologies have yielded great improvements to the knowledge of the interaction between microorganisms and their hosts, notably a better understanding of the establishment of infectious processes. Still today, the intricacies of the dialog between parasites, cells and tissues remain limited. Some improvements have been attained with the stable integration and expression of the green fluorescence protein or firefly luciferase and other reporter genes, which have allowed to better approach the monitoring of gene expression and protein localization in vivo, in situ and in real time. Read More

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http://dx.doi.org/10.1016/j.parint.2013.07.011DOI Listing
February 2014

Cross-column prediction of gas-chromatographic retention of polybrominated diphenyl ethers.

J Chromatogr A 2013 Jul 13;1298:118-31. Epub 2013 May 13.

Dipartimento di Scienze Fisiche e Chimiche, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

In this paper, we predict the retention of polybrominated diphenyl ethers (PBDEs) in capillary gas-chromatography (GC) within a useful range of separation conditions. In a first stage of this study, quantitative structure-retention relationships (QSRRs) of PBDEs in six stationary phases with different polarity are established. The single-column QSRR models are generated using the retention data of 126 PBDE congeners by multilinear regression (MLR) coupled to genetic algorithm variable selection applied to a large set of theoretical molecular descriptors of different classes. Read More

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http://dx.doi.org/10.1016/j.chroma.2013.05.018DOI Listing
July 2013
2 Reads

Combined approaches for drug design points the way to novel proline racemase inhibitor candidates to fight Chagas' disease.

PLoS One 2013 16;8(4):e60955. Epub 2013 Apr 16.

Laboratoire des Processus Infectieux à Trypanosomatidés, Département Infection et Epidémiologie, Institut Pasteur, Paris, France.

Chagas' disease is caused by Trypanosoma cruzi, a protozoan transmitted to humans by blood-feeding insects, blood transfusion or congenitally. Previous research led us to discover a parasite proline racemase (TcPRAC) and to establish its validity as a target for the design of new chemotherapies against the disease, including its chronic form. A known inhibitor of proline racemases, 2-pyrrolecarboxylic acid (PYC), is water-insoluble. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0060955PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628851PMC
November 2013
2 Reads

Non-invasive in vivo study of the Trypanosoma vivax infectious process consolidates the brain commitment in late infections.

PLoS Negl Trop Dis 2013 3;7(1):e1976. Epub 2013 Jan 3.

Institut Pasteur, Laboratoire des Processus Infectieux à Trypanosoma, Department of Infection and Epidemiology, Paris, France.

Trypanosoma vivax, one of the leading parasites responsible for Animal African Trypanosomosis (Nagana), is generally cyclically transmitted by Glossina spp. but in areas devoid of the tsetse flies in Africa or in Latin American countries is mechanically transmitted across vertebrate hosts by other haematophagous insects, including tabanids. We followed on from our recent studies on the maintenance of this parasite in vivo and in vitro, and its genetic manipulation, by constructing a West African IL1392 T. Read More

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http://dx.doi.org/10.1371/journal.pntd.0001976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536815PMC
June 2013
5 Reads

Quantitative structure/eluent-retention relationships in reversed-phase high-performance liquid chromatography based on the solvatochromic method.

Anal Bioanal Chem 2013 Jan 4;405(2-3):755-66. Epub 2012 Jul 4.

Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università degli Studi dell'Aquila, L'Aquila, Italy.

Some predictive approaches aimed at modelling the combined effect of solute molecular structure and mobile phase composition on retention in reversed-phase high-performance chromatography (RP-HPLC) have been developed in the literature. These models are established for a given binary eluent (normally acetonitrile-water or methanol-water) by non-linear (curvilinear or artificial neural network) regression assuming as the mobile phase descriptor the volume fraction φ of the organic modifier. In the present investigation, we propose a model applicable simultaneously to acetonitrile-water and methanol-water eluents. Read More

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http://dx.doi.org/10.1007/s00216-012-6191-4DOI Listing
January 2013

Changes in the carpal tunnel while wearing the Manu® soft hand brace: a sonographic study.

J Hand Surg Eur Vol 2013 Jan 28;38(1):57-60. Epub 2012 May 28.

Neurology Operative Unit, Ospedale Civile, Teramo, Italy.

We studied the effect of the Manu(®) soft hand brace, which has been designed to relieve median nerve entrapment in carpal tunnel syndrome. An observational, controlled study was conducted in 10 participants, five with bilateral carpal tunnel syndrome and five controls, using sonography to study changes in the dimensions of the carpal tunnel before and while wearing the brace. An increase in transverse diameter, thinning of the flexor retinaculum, and displacement of the proximal insertion of the lumbrical muscle to the middle finger from the edge of the carpal tunnel were observed in patients while wearing the brace. Read More

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http://dx.doi.org/10.1177/1753193412446112DOI Listing
January 2013
1 Read

Protocatechuic acid and human disease prevention: biological activities and molecular mechanisms.

Curr Med Chem 2012 ;19(18):2901-17

Dept. Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161 Rome, Italy.

Epidemiological evidence has shown that a high dietary intake of vegetables and fruit rich in polyphenols is associated with a reduction of cancer incidence and mortality from coronary heart disease. The healthy effects associated with polyphenol consumption have made the study of the mechanisms of action a matter of great importance. In particular, the hydroxybenzoic acid protocatechuic acid (PCA) has been eliciting a growing interest for several reasons. Read More

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October 2012

Cross-column retention prediction in reversed-phase high-performance liquid chromatography by artificial neural network modelling.

Anal Chim Acta 2012 Mar 31;717:52-60. Epub 2011 Dec 31.

Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università degli Studi dell'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy.

Linear solvation energy relationships (LSERs) are commonly applied to model the effect of solute structure on the retention of analytes in reversed-phase high-performance liquid chromatography (RP-HPLC). Standard LSER approaches can be used, in principle, to predict RP-HPLC behaviour of unknown analytes under fixed separation condition. However, as solute structure is the only source of variability described by the model, a LSER established for a given column/eluent pair cannot be transferred to external separation conditions. Read More

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http://dx.doi.org/10.1016/j.aca.2011.12.047DOI Listing

Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions.

PLoS Negl Trop Dis 2011 Dec 27;5(12):e1461. Epub 2011 Dec 27.

Laboratoire des Processus Infectieux à Trypanosoma, Department of Infection and Epidemiology, Paris, France.

Trypanosoma vivax is one of the most common parasites responsible for animal trypanosomosis, and although this disease is widespread in Africa and Latin America, very few studies have been conducted on the parasite's biology. This is in part due to the fact that no reproducible experimental methods had been developed to maintain the different evolutive forms of this trypanosome under laboratory conditions. Appropriate protocols were developed in the 1990s for the axenic maintenance of three major animal Trypanosoma species: T. Read More

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http://dx.doi.org/10.1371/journal.pntd.0001461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246432PMC
December 2011
2 Reads