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    DJ-1 activates autophagy in the repression of cardiac hypertrophy.
    Arch Biochem Biophys 2017 Sep 20. Epub 2017 Sep 20.
    Department of Cardiology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; Key Laboratory on Assisted Circulation, Ministry of Health, Guangzhou, China. Electronic address:
    Cardiac hypertrophy is the risk factor of heart failure when the heart is confronted with pressure overload or neurohumoral stimuli. Autophagy, a conserved degradative pathway, is one of the important mechanisms involved in the regulation of cardiac hypertrophy. DJ-1 is a traditional anti-oxidative protein and emerging evidence suggested that DJ-1 might modulate autophagy. Read More

    CFTR modulates RPS27 gene expression using chloride anion as signaling effector.
    Arch Biochem Biophys 2017 Sep 20. Epub 2017 Sep 20.
    Institute for Biomedical Research (BIOMED, UCA-CONICET), Laboratory of Cellular and Molecular Biology, School of Medical Sciences, Pontifical Catholic University of Argentina (UCA), The National Scientific and Technical Research Council of Argentina (CONICET), Buenos Aires, C1107AFF, Argentina. Electronic address:
    In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Cl(-) concentrations ([Cl(-)]i), we observed several Cl(-)-dependent genes and further characterized one of them as RPS27. Read More

    Reaction rates of glutathione and ascorbate with alkyl radicals are too slow for protection against protein peroxidation in vivo.
    Arch Biochem Biophys 2017 Sep 19. Epub 2017 Sep 19.
    Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
    Reaction kinetics of amino acid and peptide alkyl radicals with GSH and ascorbate, the two most abundant endogenous antioxidants, were investigated by pulse radiolysis. Rate constants in the order of 10(6) M(-1)s(-1) were found. Alkyl radicals react at almost diffusion controlled rates and irreversibly with oxygen to form peroxyl radicals, and competition with this reaction is the benchmark for efficient repair in vivo. Read More

    Retinol saturase modulates lipid metabolism and the production of reactive oxygen species.
    Arch Biochem Biophys 2017 Sep 18;633:93-102. Epub 2017 Sep 18.
    Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS 66045, USA. Electronic address:
    Retinol saturase (RetSat) catalyzes the saturation of double bonds of all-trans-retinol leading to the production of dihydroretinoid metabolites. Beside its role in retinoid metabolism, there is evidence that RetSat modulates the cellular response to oxidative stress and plays critical roles in adipogenesis and the accumulation of lipids. Here, we explore the relationship between RetSat, lipid metabolism and oxidative stress using in vitro and in vivo models with altered expression of RetSat. Read More

    ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activity.
    Arch Biochem Biophys 2017 Sep 14;633:85-92. Epub 2017 Sep 14.
    Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. Electronic address:
    The genome of Methanosarcinales organisms presents both ADP-dependent glucokinase and phosphofructokinase genes. However, Methanococcoides burtonii has a truncate glucokinase gene with a large deletion at the C-terminal, where the catalytic GXGD motif is located. Characterization of its phosphofructokinase annotated protein shows that is a bifunctional enzyme able to supply the absence of the glucokinase activity. Read More

    ATP alters protein folding and function of Escherichia coli uridine phosphorylase.
    Arch Biochem Biophys 2017 Sep 13. Epub 2017 Sep 13.
    Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan, ROC. Electronic address:
    Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Read More

    The soluble domains of Gpi8 and Gaa1, two subunits of glycosylphosphatidylinositol transamidase (GPI-T), assemble into a complex.
    Arch Biochem Biophys 2017 Sep 8;633:58-67. Epub 2017 Sep 8.
    Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA; Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA. Electronic address:
    Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active site subunit of GPI-T, is part of a hetero-pentameric complex containing Gpi16, Gaa1, Gpi17, and Gab1. Gpi8, Gaa1, and Gpi16 co-purify as a heterotrimer from Saccharomyces cerevisiae, suggesting that they form the core of the GPI-T. Read More

    Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells.
    Arch Biochem Biophys 2017 Sep 9;633:68-77. Epub 2017 Sep 9.
    Center of Medical Physics and Technology, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Anhui Province, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou, Jiangsu, China. Electronic address:
    Non-thermal plasma (NTP) has been proposed as a novel therapeutic method for anticancer treatment. Although increasing evidence suggests that NTP selectively induces apoptosis in some types of tumor cells, the molecular mechanisms underlying this phenomenon remain unclear. In this study, we further investigated possible molecular mechanisms for NTP-induced apoptosis of HeLa cells. Read More

    The catalytic activity of a recombinant single chain variable fragment nucleic acid-hydrolysing antibody varies with fusion tag and expression host.
    Arch Biochem Biophys 2017 Sep 6. Epub 2017 Sep 6.
    Department of Biomedical Sciences, Graduate School, Ajou University, 206 World Cup-ro, Yeongtong-gu, Suwon 16499, South Korea; Department of Microbiology, Ajou University School of Medicine, 206 World Cup-ro, Yeongtong-gu, Suwon 16499, South Korea. Electronic address:
    The antigen-binding properties of single chain Fv antibodies (scFvs) can vary depending on the position and type of fusion tag used, as well as the host cells used for expression. The issue is even more complicated with a catalytic scFv antibody that binds and hydrolyses a specific antigen. Herein, we investigated the antigen-binding and -hydrolysing activities of the catalytic anti-nucleic acid antibody 3D8 scFv expressed in Escherichia coli or HEK293f cells with or without additional amino acid residues at the N- and C-termini. Read More

    Interplay of mitochondria apoptosis regulatory factors and microRNAs in valvular heart disease.
    Arch Biochem Biophys 2017 Sep 6;633:50-57. Epub 2017 Sep 6.
    Signal Transduction Lab, Department of Bio-Chemistry, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan. Electronic address:
    Valvular heart disease (VHD) is an active process involving a wide range of pathological changes. The major complications of VHD are stenosis and regurgitation, which are macroscopic phenomena, induced in part through cellular changes. Altered expression of mitochondria associated genes causes membrane potential depolarization, leading to the increased levels of apoptosis observed in cardiac dysfunction. Read More

    Disulfiram is a slow-binding partial noncompetitive inhibitor of 20S proteasome activity.
    Arch Biochem Biophys 2017 Sep 5;633:23-28. Epub 2017 Sep 5.
    College of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, Manitoba R3E 0T5, Canada.
    The alcohol abuse drug disulfiram has also been shown to exhibit potent cell growth inhibitory and anticancer activity. While a number of cellular and animal studies have suggested that disulfiram exhibits its anticancer activity through interaction with the proteasome, direct evidence for inhibition of proteasome activity is lacking. In this study we show that disulfiram potently inhibits the chymotrypsin-like activity of purified human 20S proteasome at low micromolar pharmacological concentrations. Read More

    Bisphenol A and estrogen induce proliferation of human thyroid tumor cells via an estrogen-receptor-dependent pathway.
    Arch Biochem Biophys 2017 Sep 4;633:29-39. Epub 2017 Sep 4.
    Department of Endocrinology, Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address:
    Objective: To determine the relationship between papillary thyroid carcinoma and environmental exposure to bisphenol A (BPA) or 17-β estrogen (E2) by assessing the effects of these compounds on estrogen receptor expression and AKT/mTOR signaling.

    Methods: The effects of low levels of BPA (1mM-10nM) and 17β-estradiol (E2, 0.1mM-1nM) on ER expression and cellular proliferation were determined in human thyroid papillary cancer BHP10-3 cells. Read More

    Effect of conservative mutations (L94V and L94I) on the structure and stability of horse cytochrome c.
    Arch Biochem Biophys 2017 Aug 26;633:40-49. Epub 2017 Aug 26.
    Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India. Electronic address:
    A sequence alignment of horse cytochrome c (cyt c) with all known cyts c shows that Leu at position 94 is conserved, except in 14 species which have either Val or Ile at this position. It is also known that Leu94 of the mammalian cyt c plays an important role in folding and stability. The important question here is as to what will happen in terms of folding and stability if Leu94 of the mammalian cyt c is substituted by Val or Ile. Read More

    Mechanistic insights into EgGST1, a Mu class glutathione S-transferase from the cestode parasite Echinococcus granulosus.
    Arch Biochem Biophys 2017 Aug 30;633:15-22. Epub 2017 Aug 30.
    Cátedra de Inmunología/DEPBIO, Facultad de Química, Universidad de la República, Montevideo, 11600, Uruguay. Electronic address:
    Glutathione transferases (GSTs) comprise a major detoxification system in helminth parasites, displaying both catalytic and non-catalytic activities. The kinetic mechanism of these enzymes is complex and depends on the isoenzyme which is being analyzed. Here, we characterized the kinetic mechanism of rEgGST1, a recombinant form of a cytosolic GST from Echinococcus granulosus (EgGST1), which is related to the Mu-class of mammalian enzymes, using the canonical substrates glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). Read More

    Thiol dioxygenase turnover yields benzothiazole products from 2-mercaptoaniline and O2-dependent oxidation of primary alcohols.
    Arch Biochem Biophys 2017 Oct 18;631:66-74. Epub 2017 Aug 18.
    Department of Chemistry & Biochemistry, College of Science, The University of Texas at Arlington, Arlington, TX 76019, United States. Electronic address:
    Thiol dioxygenases are non-heme mononuclear iron enzymes that catalyze the O2-dependent oxidation of free thiols (-SH) to produce the corresponding sulfinic acid (-SO2(-)). Previous chemical rescue studies identified a putative Fe(III)-O2(-) intermediate that precedes substrate oxidation in Mus musculus cysteine dioxygenase (Mm CDO). Given that a similar reactive intermediate has been identified in the extradiol dioxygenase 2, 3-HCPD, it is conceivable that these enzymes share other mechanistic features with regard to substrate oxidation. Read More

    Role of ADP ribosylation factor6- Cytohesin1-PhospholipaseD signaling axis in U46619 induced activation of NADPH oxidase in pulmonary artery smooth muscle cell membrane.
    Arch Biochem Biophys 2017 Aug 16;633:1-14. Epub 2017 Aug 16.
    Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address:
    Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cell membrane contains predominantly PLD2 along with PLD1 isoforms of PLD. Read More

    Flavin-dependent thymidylate synthase: N5 of flavin as a Methylene carrier.
    Arch Biochem Biophys 2017 Aug 15. Epub 2017 Aug 15.
    Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA.
    Thymidylate is synthesized de novo in all living organisms for replication of genomes. The chemical transformation is reductive methylation of deoxyuridylate at C5 to form deoxythymidylate. All eukaryotes including humans complete this well-understood transformation with thymidylate synthase utilizing (6R)-N(5)-N(10)-methylene-5,6,7,8-tetrahydrofolate as both a source of methylene and a reducing hydride. Read More

    Identification and quantification and antioxidant activity of flavonoids in different strains of silk cocoon, Bombyx mori.
    Arch Biochem Biophys 2017 Oct 12;631:58-65. Epub 2017 Aug 12.
    Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University, Phatumwan, Bangkok 10330, Thailand; Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand. Electronic address:
    Silk cocoon is produced from silkworm (Bombyx mori) to protect itself from outer environment. Various strains of cocoon contain different forms and amounts of flavonoids, which may affect on their antioxidant activity. Moreover, the extraction method would influence the amount of flavonoids extracted. Read More

    Silencing of Glut1 induces chemoresistance via modulation of Akt/GSK-3β/β-catenin/survivin signaling pathway in breast cancer cells.
    Arch Biochem Biophys 2017 Aug 10. Epub 2017 Aug 10.
    Department of Life Science, Hanyang University, Seoul, 133-791, South Korea; Natural Science Institute, Hanyang University, Seoul, 133-791, South Korea. Electronic address:
    Cancer cells require increased aerobic glycolysis to support rapid cell proliferation. For their increased energy demands, cancer cells express glucose transporter (Glut) proteins at a high level. Glut1 is associated with basal-like breast cancer and is considered a potential therapeutic target. Read More

    Methionine sulfoxide reductase A protects against lipopolysaccharide-induced septic shock via negative regulation of the proinflammatory responses.
    Arch Biochem Biophys 2017 Oct 10;631:42-48. Epub 2017 Aug 10.
    Department of Biochemistry and Molecular Biology, Yeungnam University College of Medicine, Daegu, Republic of Korea. Electronic address:
    Methionine sulfoxide reductase A (MsrA) is a major antioxidant enzyme that specifically catalyzes the reduction of methionine S-sulfoxide. In this study, we used MsrA gene-knockout (MsrA(-/-)) mice and bone marrow-derived macrophages (BMDMs) to investigate the role of MsrA in the regulation of inflammatory responses induced by lipopolysaccharide (LPS). MsrA(-/-) mice were more susceptible to LPS-induced lethal shock than wild-type (MsrA(+/+)) mice. Read More

    Photolyase: Dynamics and electron-transfer mechanisms of DNA repair.
    Arch Biochem Biophys 2017 Aug 9. Epub 2017 Aug 9.
    Department of Physics, Department of Chemistry and Biochemistry, Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University, Columbus, OH 43210, USA. Electronic address:
    Photolyase, a flavoenzyme containing flavin adenine dinucleotide (FAD) molecule as a catalytic cofactor, repairs UV-induced DNA damage of cyclobutane pyrimidine dimer (CPD) and pyrimidine-pyrimidone (6-4) photoproduct using blue light. The FAD cofactor, conserved in the whole protein superfamily of photolyase/cryptochromes, adopts a unique folded configuration at the active site that plays a critical functional role in DNA repair. Here, we review our comprehensive characterization of the dynamics of flavin cofactor and its repair photocycles by different classes of photolyases on the most fundamental level. Read More

    The novel heme-dependent inducible protein, SRRD regulates heme biosynthesis and circadian rhythms.
    Arch Biochem Biophys 2017 Oct 9;631:19-29. Epub 2017 Aug 9.
    Department of Biotechnology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan; Units of Research Complex, Kansai Medical University, Hirakata, Osaka 573-1010 Japan. Electronic address:
    Heme plays a role in the regulation of the expression of genes related to circadian rhythms and heme metabolism. In order to identify new heme-regulated proteins, an RNA sequence analysis using mouse NIH3T3 cells treated without or with 5-aminolevulinic acid (ALA) was performed. Among the changes observed in the levels of various mRNAs including heme oxygenase-1 (HO-1) and ALA synthase-1 (ALAS1), a mouse homologue of the plant circadian-regulating protein SRR1, SRR1 domain containing (SRRD) was induced by the ALA treatment. Read More

    Nuclear factor (erythroid-derived 2)-like 2 (NRF2) drug discovery: Biochemical toolbox to develop NRF2 activators by reversible binding of Kelch-like ECH-associated protein 1 (KEAP1).
    Arch Biochem Biophys 2017 Oct 8;631:31-41. Epub 2017 Aug 8.
    CHDI Management/CHDI Foundation, Los Angeles, CA, USA. Electronic address:
    Mechanisms that activate innate antioxidant responses, as a way to mitigate oxidative stress at the site of action, hold much therapeutic potential in diseases, such as Parkinson's disease, Alzheimer's disease and Huntington's disease, where the use of antioxidants as monotherapy has not yielded positive results. The nuclear factor NRF2 is a transcription factor whose activity upregulates the expression of cell detoxifying enzymes in response to oxidative stress. NRF2 levels are modulated by KEAP1, a sensor of oxidative stress. Read More

    Circadian rhythmicity: A functional connection between differentiated embryonic chondrocyte-1 (DEC1) and small heterodimer partner (SHP).
    Arch Biochem Biophys 2017 Oct 8;631:11-18. Epub 2017 Aug 8.
    Department of Biomedical and Pharmaceutical Sciences, Center for Integrated Drug Development, University of Rhode Island, Kingston, RI 02881, United States. Electronic address:
    Circadian rhythm misalignment has been increasingly recognized to pose health risk for a wide range of diseases, particularly metabolic disorders. The liver maintains metabolic homeostasis and expresses many circadian genes, such as differentiated embryo chondrocyte-1 (DEC1) and small heterodimer partner (SHP). DEC1 is established to repress transcription through E-box elements, and SHP belongs to the superfamily of nuclear receptors and has multiple E-box elements in its promoter. Read More

    Tetramer as efficient structural mode for organizing antioxidative carboxylic acids: The case in inhibiting DNA oxidation.
    Arch Biochem Biophys 2017 Oct 5;631:1-10. Epub 2017 Aug 5.
    Department of Organic Chemistry, College of Chemistry, Jilin University, Changchun 130021, China. Electronic address:
    To overcome the problem on the relationship of antioxidative effect with the branch number in a tetramer, we herein designed a series of antioxidants with pentaerythritol, glycerol, and ethylene glycol as the cores, and gallic, ferulic, caffeic, and p-hydroxybenzoic acids as the antioxidative moieties. In the case of DNA oxidation mediated by 2,2'-azobis(2-amidinopropane hydrochloride, AAPH), it was found that the stoichiometric factor (n) of a carboxylic acid increased rapidly when the acid was esterified with ethylene glycol, glycerol, and pentaerythritol to form a dimer, trimer, and tetramer, respectively. Interestingly, the coefficient in the equation of n∼{branch} ({branch} referred to the number of branches) was higher than one, indicating that the antioxidative effect was enhanced more promptly than the increase of the number of branches. Read More

    Flavin-N5-oxide: A new, catalytic motif in flavoenzymology.
    Arch Biochem Biophys 2017 Aug 5. Epub 2017 Aug 5.
    Department of Chemistry, Texas A&M University, College Station, TX 77843, United States. Electronic address:
    Flavin-N5-oxide is a recently discovered intermediate used by EncM (1,3-diketone oxidation), DszA (sulfone monooxygenase) and RutA (amide monooxygenase). This review describes the mechanism of these enzymes and proposes criteria for the identification of additional Flavin-N5-oxide dependent enzymes. Read More

    Probing the interaction of the p53 C-terminal domain to the histone demethylase LSD1.
    Arch Biochem Biophys 2017 Aug 4. Epub 2017 Aug 4.
    Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy. Electronic address:
    The p53 transcription factor plays a central role in the regulation of the expression of several genes, and itself is post-translationally regulated through its different domains. Of particular relevance for p53 function is its intrinsically disordered C-terminal domain (CTD), representing a hotspot for post-translational modifications and a docking site for transcriptional regulators. For example, the histone H3 lysine demethylase 1 (LSD1) interacts with p53 via the p53-CTD for mutual regulation. Read More

    Automatic regulation of NF-κB by pHSP70/IκBαm to prevent acute lung injury in mice.
    Arch Biochem Biophys 2017 Aug 1. Epub 2017 Aug 1.
    Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi`an, 710032, PR China. Electronic address:
    Controlling target gene expression is a vital step in the procedure of gene therapy upon acute lung injury (ALI). Excessive activation of nuclear factor-kappa B (NF-κB) has been the key point of the inflammation overwhelming process in onset of ALI. We designed and tested a variety of plasmid named pHSP70/IκBαm which conditionally carries a mutant inhibitor of kappa B (IκB) transgene to regulate the activity of NF-κB signaling pathway in its response to an inflammatory stimulus that causes acute lung injury. Read More

    The distribution and mechanism of iodotyrosine deiodinase defied expectations.
    Arch Biochem Biophys 2017 Jul 31. Epub 2017 Jul 31.
    Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA. Electronic address:
    Iodotyrosine deiodinase (IYD) is unusual for its reliance on flavin to promote reductive dehalogenation under aerobic conditions. As implied by the name, this enzyme was first discovered to catalyze iodide elimination from iodotyrosine for recycling iodide during synthesis of tetra- and triiodothyronine collectively known as thyroid hormone. However, IYD likely supports many more functions and has been shown to debrominate and dechlorinate bromo- and chlorotyrosines. Read More

    2-methoxyethylacrylate modified polysulfone membrane and its blood compatibility.
    Arch Biochem Biophys 2017 Oct 29;631:49-57. Epub 2017 Jul 29.
    College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address:
    Hydrophilic material 2-methoxyethylacrylate (MEA) was grafted onto polysulfone (PSF) membrane via Michael addition reaction. The (1)H nuclear magnetic resonance ((1)H NMR), Attenuated total reflectance-Fourier transform infrared spectroscope (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) characterization of the modified membrane proved that MEA had been successfully grafted onto PSF membrane surface. The water contact angle of the membrane surface was tested. Read More

    Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior.
    Arch Biochem Biophys 2017 Sep 29;630:91-100. Epub 2017 Jul 29.
    Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Department of Biomolecular Science and Engineering, School of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong 21210, Thailand. Electronic address:
    Serine hydroxymethyltransferase (SHMT), an essential enzyme for cell growth and development, catalyzes the transfer of -CH2OH from l-serine to tetrahydrofolate (THF) to form glycine and 5,10-methylenetetrahydrofolate (MTHF) which is used for nucleotide synthesis. Insights into the ligand binding and inhibition properties of human cytosolic SHMT (hcSHMT) and Plasmodium SHMT (PvSHMT) are crucial for designing specific drugs against malaria and cancer. The results presented here revealed strong and pH-dependent THF inhibition of hcSHMT. Read More

    l-Cysteine supplementation increases insulin sensitivity mediated by upregulation of GSH and adiponectin in high glucose treated 3T3-L1 adipocytes.
    Arch Biochem Biophys 2017 Sep 27;630:54-65. Epub 2017 Jul 27.
    Department of Pediatrics, Louisiana State University Health Sciences Center, Shreveport, LA 71103, United States. Electronic address:
    Diabetic patients have lower blood levels of l-cysteine (LC) and glutathione (GSH). This study examined the hypothesis that LC supplementation positively up regulates the effects of insulin on GSH and glucose metabolism in 3T3-L1 adipocyte model. 3T3L1 adipocytes were treated with LC (250 μM, 2 h) and/or insulin (15 or 30 nM, 2 h), and high glucose (HG, 25 mM, 20 h). Read More

    The UbiX-UbiD system: The biosynthesis and use of prenylated flavin (prFMN).
    Arch Biochem Biophys 2017 Jul 25. Epub 2017 Jul 25.
    Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Electronic address:
    The UbiX-UbiD system consists of the flavin prenyltransferase UbiX that produces prenylated FMN that serves as the cofactor for the (de)carboxylase UbiD. Recent developments have provided structural insights into the mechanism of both enzymes, detailing unusual chemistry in each case. The proposed reversible 1,3-dipolar cycloaddition between the cofactor and substrate serves as a model to explain many of the key UbiD family features. Read More

    Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a.
    Arch Biochem Biophys 2017 Sep 25;630:38-46. Epub 2017 Jul 25.
    Department of Nutritional Sciences and Toxicology, University of California Berkeley, Berkeley, CA 94720, USA; Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA. Electronic address:
    Wnt signaling is essential for embryonic development and adult homeostasis in multicellular organisms. A conserved feature among Wnt family proteins is the presence of two structural domains. Within the N-terminal (NT) domain there exists a motif that is superimposable upon saposin-like protein (SAPLIP) family members. Read More

    Crystal structure of Pisum arvense seed lectin (PAL) and characterization of its interaction with carbohydrates by molecular docking and dynamics.
    Arch Biochem Biophys 2017 Sep 25;630:27-37. Epub 2017 Jul 25.
    Universidade Federal do Ceara (UFC), Fortaleza, Ceara, Brazil. Electronic address:
    The Pisum arvense lectin (PAL), a legume protein belonging to the Vicieae tribe, is capable of specific recognition of mannose, glucose and its derivatives without altering its structure. In this work, the three-dimensional structure of PAL was determined by X-ray crystallography and studied in detail by a combination of molecular docking and molecular dynamics (MD). Crystals belonging to monoclinic space group P21 were grown by the vapor diffusion method at 293 K. Read More

    Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia-inducible factor 1α in human dermal fibroblasts.
    Arch Biochem Biophys 2017 Sep 24;630:9-17. Epub 2017 Jul 24.
    Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, South Korea. Electronic address:
    Numerous studies on the application of low temperature plasma (LTP) have produced impressive results, including antimicrobial, antitumor, and wound healing effects. Although LTP research has branched out to include medical applications, the detailed effects and working mechanisms of LTP on wound healing have not been fully investigated. Here, we investigated the potential effect of inducing growth factor after exposure to LTP and demonstrated the increased expression of angiogenic growth factor mediated by LTP-induced HIF1α expression in primary cultured human dermal fibroblasts. Read More

    Association between ROS production, swelling and the respirasome integrity in cardiac mitochondria.
    Arch Biochem Biophys 2017 Sep 20;630:1-8. Epub 2017 Jul 20.
    Department of Physiology, University of Puerto Rico School of Medicine, San Juan, Puerto Rico. Electronic address:
    Although mitochondrial Ca(2+) overload and ROS production play a critical role in mitochondria-mediated cell death, a cause-effect relationship between them remains elusive. This study elucidated the crosstalk between mitochondrial swelling, ROS production, and electron transfer chain (ETC) supercomplexes in rat heart mitochondria in response to Ca(2+) and tert-butyl hydroperoxide (TBH), a lipid-soluble organic peroxide. Results showed that ROS production induced by TBH was significantly increased in the presence of Ca(2+) in a dose-dependent manner. Read More

    MiR-143 regulates the proliferation and migration of osteosarcoma cells through targeting MAPK7.
    Arch Biochem Biophys 2017 Sep 20;630:47-53. Epub 2017 Jul 20.
    Nanjing Medical University, Nanjing, PR China; Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China. Electronic address:
    Accumulating documents have been suggested that microRNA-143 (miR-143) function as a tumor suppressor, involved in many biological processes including tumor initiation and progression. However, the biological function and molecular mechanism of miR-143 in Osteosarcoma (OS) still remains to be further investigated. Despite many efforts have been made, the prognosis of OS is still unsatisfied. Read More

    A new PPARγ/DNA origami biochromatography and offline high performance liquid chromatography-mass spectrometry method for screening PPARγ receptor antagonists from ginsenosides.
    Arch Biochem Biophys 2017 Sep 19;629:63-69. Epub 2017 Jul 19.
    School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China; Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Zhengzhou, Henan 450001, PR China. Electronic address:
    To rapidly identify novel PPARγ ligands, a robust binding assay amenable to high-efficiency screening toward PPARγ would be desirable. In this study, a new PPARγ assembled on DNA origami (PPARγ/DNA origami) biochromatography drug screening model was constructed and evaluated. The method was used to screen active ingredients acted on PPARγ from the total ginsenosides. Read More

    The cardiomyopathy-associated K15N mutation in tropomyosin alters actin filament pointed end dynamics.
    Arch Biochem Biophys 2017 Sep 18;630:18-26. Epub 2017 Jul 18.
    Voiland School of Chemical Engineering & Bioengineering, Washington State University, Pullman, WA 99164-6515, United States. Electronic address:
    Correct assembly of thin filaments composed of actin and actin-binding proteins is of crucial importance for properly functioning muscle cells. Tropomyosin (Tpm) mediates the binding of tropomodulin (Tmod) and leiomodin (Lmod) at the slow-growing, or pointed, ends of the thin filaments. Together these proteins regulate thin filament lengths and actin dynamics in cardiac muscle. Read More

    The increase in positively charged residues in cecropin D-like Galleria mellonella favors its interaction with membrane models that imitate bacterial membranes.
    Arch Biochem Biophys 2017 Sep 15;629:54-62. Epub 2017 Jul 15.
    Universidad de Murcia, Departamento de Bioquímica y Biología Molecular-A, Facultad de Veterinaria, Campus of International Excellence Mare Nostrum, E-30100, Murcia, Spain. Electronic address:
    A comparative study of three synthetic peptides, namely neutral Cecropin D-like G. mellonella (WT) and two cationic peptides derived from its sequence, ΔM1 (+5) and ΔM2 (+9) is reported in this work. The influence of charge on the interactions between peptides and membranes and its effect on phase were studied by calorimetric assays. Read More

    Protective role of antioxidant compounds against peroxynitrite-mediated modification of R54C mutant αA-crystallin.
    Arch Biochem Biophys 2017 Sep 16;629:43-53. Epub 2017 Jul 16.
    Institute of Biochemistry and Biophysics (IBB), The University of Tehran, Tehran, Iran; UNESCO Chair on Interdisciplinary Research in Diabetes, University of Tehran, Iran.
    As a highly potent reactive oxygen and nitrogen species, peroxynitrite (PON) has endogenous production in the eye ball and contributes to a variety of ocular disorders. In the current study the structural characteristics, chaperone-like activity and conformational stability of R54C mutant αA-crystallin (αA-Cry) were studied upon modification with PON and in the presence of three antioxidant compounds such as ascorbic acid (ASA), glutathione (GSH) and N-acetylcysteine (NAC) using gel electrophoresis and different spectroscopy methods. The results of both fluorescence analysis and gel electrophoresis suggested that PON modification leads to dityrosine-mediated intermolecular cross-linking of this cataractogenic mutant protein. Read More

    Ex vivo instability of glycated albumin: A role for autoxidative glycation.
    Arch Biochem Biophys 2017 Sep 13;629:36-42. Epub 2017 Jul 13.
    School of Molecular Sciences, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, United States. Electronic address:
    Ex vivo protein modifications occur within plasma and serum (P/S) samples due to prolonged exposure to the thawed state-which includes temperatures above -30 °C. Herein, the ex vivo glycation of human serum albumin from healthy and diabetic subjects was monitored in P/S samples stored for hours to months at -80 °C, -20 °C, and room temperature, as well as in samples subjected to multiple freeze-thaw cycles, incubated at different surface area-to-volume ratios or under different atmospheric compositions. A simple dilute-and-shoot method utilizing trap-and-elute LC-ESI-MS was employed to determine the relative abundances of the glycated forms of albumin-including forms of albumin bearing more than one glucose molecule. Read More

    Structure, function, and mechanism of proline utilization A (PutA).
    Arch Biochem Biophys 2017 Jul 14. Epub 2017 Jul 14.
    Department of Biochemistry, University of Missouri, Columbia, MO, 65211, United States; Department of Chemistry, University of Missouri, Columbia, MO, 65211, United States. Electronic address:
    Proline has important roles in multiple biological processes such as cellular bioenergetics, cell growth, oxidative and osmotic stress response, protein folding and stability, and redox signaling. The proline catabolic pathway, which forms glutamate, enables organisms to utilize proline as a carbon, nitrogen, and energy source. FAD-dependent proline dehydrogenase (PRODH) and NAD(+)-dependent glutamate semialdehyde dehydrogenase (GSALDH) convert proline to glutamate in two sequential oxidative steps. Read More

    Interconversion of inactive to active conformation of MARK2: Insights from molecular modeling and molecular dynamics simulation.
    Arch Biochem Biophys 2017 Sep 12;630:66-80. Epub 2017 Jul 12.
    Biophysics and Computational Biology Laboratory (BCBL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
    The Ser/Thr protein kinase MARK2, also known as Par1b, belongs to the highly-conserved family of PAR proteins which regulate cell polarity and partitioning through the animal kingdom. In the current study, inactive and active structures of human MARK2 were constructed by modeling and molecular dynamics simulation, based on available incomplete crystal structures in Protein Data Bank, to investigate local structural changes through which MARK2 switches from inactive to active state. None of the MARK2 wild type inactive crystal structures represent the position of activation segment. Read More

    Histidine substitution in the most flexible fragments of firefly luciferase modifies its thermal stability.
    Arch Biochem Biophys 2017 Sep 12;629:8-18. Epub 2017 Jul 12.
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
    Molecular dynamics (MD) at two temperatures of 300 and 340 K identified two histidine residues, His461 and His489, in the most flexible regions of firefly luciferase, a light emitting enzyme. We therefore designed four protein mutants H461D, H489K, H489D and H489M to investigate their enzyme kinetic and thermodynamic stability changes. Substitution of His461 by aspartate (H461D) decreased ATP binding affinity, reduced the melting temperature of protein by around 25 °C and shifted its optimum temperature of activity to 10 °C. Read More

    The nitroxide 4-methoxy TEMPO inhibits neutrophil-stimulated kinase activation in H9c2 cardiomyocytes.
    Arch Biochem Biophys 2017 Sep 5;629:19-35. Epub 2017 Jul 5.
    Discipline of Pathology, Charles Perkins Centre, Sydney Medical School, The University of Sydney, NSW 2006, Australia. Electronic address:
    After acute myocardial infarction (AMI), neutrophils are recruited to the affected myocardium. Hypochlorous acid (HOCl) produced by neutrophil myeloperoxidase (MPO) damages cardiomyocytes and potentially expands the primary infarct. Rat cardiomyocyte-like cells were incubated with isolated human neutrophils treated with chemical activators in the absence or presence of nitroxide 4-methoxy-Tempo (MetT; 25 μM) for 4, 6 or 24 h; studies with reagent HOCl served as positive control. Read More

    The family of berberine bridge enzyme-like enzymes: A treasure-trove of oxidative reactions.
    Arch Biochem Biophys 2017 Jul 1. Epub 2017 Jul 1.
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, A-8010 Graz, Austria. Electronic address:
    Biological oxidations form the basis of life on earth by utilizing organic compounds as electron donors to drive the generation of metabolic energy carriers, such as ATP. Oxidative reactions are also important for the biosynthesis of complex compounds, i.e. Read More

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