29,360 results match your criteria Archives of Biochemistry and Biophysics[Journal]


Simvastatin promotes restoration of chondrocyte morphology and phenotype.

Arch Biochem Biophys 2019 Feb 15. Epub 2019 Feb 15.

Department of Anatomy and Cell Biology, East Carolina University, 600 Moye Blvd., Greenville, NC, 27834, USA. Electronic address:

In this study we examined whether the action of simvastatin affects re-differentiation of passaged chondrocytes and if so, whether this was mediated via changes in cholesterol or cholesterol intermediates. Bovine articular chondrocytes, of varying passage number, human knee chondrocytes and rat chondrosarcoma chondrocytes were treated with simvastatin and examined for changes in mRNA and protein expression of markers of the chondrocyte phenotype as well as changes in cell shape, proliferation and proteoglycan production. In all three models, while still in monolayer culture, simvastatin treatment alone promoted changes in phenotype and morphology indicative of re-differentiation most prominent being an increase in SOX9 mRNA and protein expression. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.038DOI Listing
February 2019

Protein phosphatase PPP3CA (calcineurin A) down-regulates hypoxia-inducible factor transcriptional activity.

Arch Biochem Biophys 2019 Feb 15. Epub 2019 Feb 15.

Laboratory of Biochemistry, Faculty of Medicine, University of Thessaly, Larissa, Greece. Electronic address:

Hypoxia-inducible factors (HIF) are master regulators of the response to hypoxia. Although several kinases are known to modify their oxygen sensitive HIF-α subunits or affect indirectly their function, little is known about the role of phosphatases in HIF control. To address this issue, a library containing siRNAs for the 25 known catalytic subunits of human phosphatases was used to screen for their effect on HIF transcriptional activity in HeLa cells. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.007DOI Listing
February 2019

Purification and biophysical characterization of a mannose/N-acetyl-d-glucosamine-specific lectin from Machaerium acutifolium and its effect on inhibition of orofacial pain via TRPV1 receptor.

Arch Biochem Biophys 2019 Feb 14. Epub 2019 Feb 14.

Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil. Electronic address:

A new mannose/N-acetyl-dglucosamine-specific lectin, named MaL, was purified from seeds of Machaerium acutifolium by precipitation with ammonium sulfate, followed by affinity and ion-exchange chromatography. MaL haemagglutinates either native rabbit erythrocytes or those treated with proteolytic enzymes. MaL is highly stable by the ability to maintain its haemagglutinating activity after exposure to temperatures up to 50 °C. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.009DOI Listing
February 2019

NOD-like receptors and inflammasomes: A review of their canonical and non-canonical signaling pathways.

Arch Biochem Biophys 2019 Feb 14. Epub 2019 Feb 14.

Department of Medicine, University of Calgary, Calgary, AB, Canada; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada. Electronic address:

The NOD-like receptor (NLR) family of proteins is a group of pattern recognition receptors (PRRs) known to mediate the initial innate immune response to cellular injury and stress. The NLRP proteins represent a fourteen-member subset of the NLR family that contains an N-terminal pyrin domain. Some NLRs are known to form multi-protein complexes known as inflammasomes. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.008DOI Listing
February 2019

Glabridin inhibits dexamethasone-induced muscle atrophy.

Arch Biochem Biophys 2019 Feb 13. Epub 2019 Feb 13.

Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 651-8501, Japan. Electronic address:

Prevention of muscle wasting is known to contribute to improving the quality of life and extending a healthy life. Recently, we have reported that licorice flavonoid oil containing glabridin, which is a prenylated isoflavone, enhances muscle mass in mice. In this study, we investigated the prevention effect of glabridin on dexamethasone-induced muscle atrophy and clarified its mechanism in cultured myotubes and in muscle of mice. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.006DOI Listing
February 2019

Biochemical characterization of TyrA dehydrogenases from Saccharomyces cerevisiae (Ascomycota) and Pleurotus ostreatus (Basidiomycota).

Arch Biochem Biophys 2019 Feb 13. Epub 2019 Feb 13.

Department of Botany, University of Wisconsin-Madison, Madison, WI, 53706, USA.

L-Tyrosine is an aromatic amino acid necessary for protein synthesis in all living organisms and a precursor of secondary (specialized) metabolites. In fungi, tyrosine-derived compounds are associated with virulence and defense (i.e. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.005DOI Listing
February 2019

The effect of the branched chain polypeptide carrier on biodistribution of covalently attached B-cell epitope peptide (APDTRPAPG) derived from mucin 1 glycoprotein.

Arch Biochem Biophys 2019 Feb 8;664:127-133. Epub 2019 Feb 8.

MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, Budapest 112, P.O. Box 32, H-1518, Hungary; Department of Organic Chemistry, Eötvös Loránd University, Budapest 112, P.O. Box 32, H-1518, Hungary.

In order to establish structure-function relationship for the design of a new group of oligopeptide antigen-macromolecule conjugate, multiple copies of mucin-1 B-cell epitope peptide, APDTRPAPG were conjugated with branched chain polymeric polypeptides possessing poly[L-Lys] backbone. By the synthesis, radiolabeling (I) and in vivo treatment of BALB/c mice with epitope conjugates containing XK/XAK type carrier, where X = Glu (EK or EAK) or Leu (LAK), the influence of the polypeptide structure on the blood clearance profile and on tissue distribution profile concerning the epitope delivery to relevant organs (e.g. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.003DOI Listing
February 2019

Atomic force microscopy for single molecule characterisation of protein aggregation.

Arch Biochem Biophys 2019 Feb 8;664:134-148. Epub 2019 Feb 8.

Centre for Misfolding Disease, Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom; Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, United Kingdom. Electronic address:

The development of atomic force microscopy (AFM) has opened up a wide range of novel opportunities in nanoscience and new modalities of observation in complex biological systems. AFM imaging has been widely employed to resolve the complex and heterogeneous conformational states involved in protein aggregation at the single molecule scale and shed light onto the molecular basis of a variety of human pathologies, including neurodegenerative disorders. The study of individual macromolecules at nanoscale, however, remains challenging, especially when fully quantitative information is required. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.001DOI Listing
February 2019

The impact of water molecules on binding affinity of the anti-diabetic thiazolidinediones for catalase: Kinetic and mechanistic approaches.

Arch Biochem Biophys 2019 Feb 7;664:110-116. Epub 2019 Feb 7.

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran; Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.

Water molecules play a vital role in efficient drug binding to its target. Thiazolidinediones (TZDs), a class of anti-diabetic drugs, are widely used for treatment of type 2 diabetes mellitus. In the present study, the possible contribution of water molecules to the binding of TZDs to catalase, a potential target in the liver, was investigated by different experimental and theoretical methods. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.002DOI Listing
February 2019

Transport of cystine across xC antiporter.

Arch Biochem Biophys 2019 Feb 6;664:117-126. Epub 2019 Feb 6.

Research Group PLASMANT, Department of Chemistry, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium.

Extracellular cystine (CYC) uptake by xC antiporter is important for the cell viability. Especially in cancer cells, the upregulation of xC activity is observed, which protects these cells from intracellular oxidative stress. Hence, inhibition of the CYC uptake may eventually lead to cancer cell death. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.039DOI Listing
February 2019
1 Read

Skeletal muscle excitation-metabolism coupling.

Arch Biochem Biophys 2019 Feb 1;664:89-94. Epub 2019 Feb 1.

Center for Studies of Exercise, Metabolism and Cancer (CEMC), ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address:

Mitochondria represent the main source of ATP in skeletal muscle and mitochondria activity increases after muscle fiber depolarization. The regulation of mitochondrial function during contraction in skeletal muscle, however, is poorly understood. Skeletal muscle has a particular distribution of mitochondria where three distinct populations can be recognized. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.037DOI Listing
February 2019

Energy-dissipating hub in muscle mitochondria: Potassium channels and uncoupling proteins.

Arch Biochem Biophys 2019 Feb 1;664:102-109. Epub 2019 Feb 1.

Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Pasteur St. 3, Warsaw, Poland. Electronic address:

Energy homeostasis in mitochondria is vital for proper muscle cell function. In this review we will focus on cardiac and skeletal muscle energy-dissipating systems, i.e. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.036DOI Listing
February 2019

Molecular simulation of peptides coming of age: Accurate prediction of folding, dynamics and structures.

Arch Biochem Biophys 2019 Feb 1;664:76-88. Epub 2019 Feb 1.

Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Alexandroupolis, 68100, Greece. Electronic address:

The application of molecular dynamics simulations to study the folding and dynamics of peptides has attracted a lot of interest in the last couple of decades. Following the successful prediction of the folding of several proteins using molecular simulation, foldable peptides emerged as a favourable system mainly due to their application in improving protein structure prediction methods and in drug design studies. However, their performance is inherently linked to the accuracy of the empirical force fields used in the simulations, whose optimisation and validation is of paramount importance. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.033DOI Listing
February 2019

Mitochondrial Rieske iron-sulfur protein in pulmonary artery smooth muscle: A key primary signaling molecule in pulmonary hypertension.

Arch Biochem Biophys 2019 Jan 30;664:68-75. Epub 2019 Jan 30.

Department of Molecular and Cellular Physiology, Albany Medical College, Albany, NY, 12208, USA. Electronic address:

Rieske iron-sulfur protein (RISP) is a catalytic subunit of the complex III in the mitochondrial electron transport chain. Studies for years have revealed that RISP is essential for the generation of intracellular reactive oxygen species (ROS) via delicate signaling pathways associated with many important molecules such as protein kinase C-ε, NADPH oxidase, and ryanodine receptors. More significantly, mitochondrial RISP-mediated ROS production has been implicated in the development of hypoxic pulmonary vasoconstriction, leading to pulmonary hypertension, right heart failure, and death. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.029DOI Listing
January 2019
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How myofilament strain and strain rate lead the dance of the cardiac cycle.

Authors:
Charles S Chung

Arch Biochem Biophys 2019 Jan 30;664:62-67. Epub 2019 Jan 30.

Department of Physiology, Wayne State University, Rm 4126 Scott Hall, 540 E Canfield, Detroit, MI, 48201, USA. Electronic address:

Movement of the myocardium can modify organ-level cardiac function and its molecular (crossbridge) mechanisms. This motion, which is defined by myocardial strain and strain rate (muscle shortening, lengthening, and the speed of these movements), occurs throughout the cardiac cycle, including during isovolumic periods. This review highlights how the left ventricular myocardium moves throughout the cardiac cycle, how muscle mechanics experiments provide insight into the regulation of forces used to move blood in and out of the left ventricle, and its impact on (and regulation by) crossbridge and sarcomere kinetics. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.034DOI Listing
January 2019
1 Read

The NLRP3 inflammasome - interleukin 1 pathway as a therapeutic target in gout.

Arch Biochem Biophys 2019 Jan 30. Epub 2019 Jan 30.

Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Electronic address:

The NLRP3 inflammasome is implicated in the processing of the pro-inflammatory cytokine interleukin 1β. Inflammatory disorders associated with the activation of the NLRP3 inflammasome - IL-1 axis are termed autoinflammatory diseases. Gout is an autoinflammatory disease, which is triggered by the deposition of monosodium urate crystals of precipitated uric acid. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.031DOI Listing
January 2019
1 Read

The isolated C-terminal nuclear localization sequence of the breast cancer metastasis suppressor 1 is disordered.

Arch Biochem Biophys 2019 Jan 30;664:95-101. Epub 2019 Jan 30.

CNR-NANOTEC, Licryl-UOS Cosenza and CEMIF.Cal, Department of Physics, University of Calabria, 87036, Rende, Italy. Electronic address:

BRMS1 is a 246-residue-long protein belonging to the family of metastasis suppressors. It is a predominantly nuclear protein, although it can also function in the cytoplasm. At its C terminus, it has a region that is predicted to be a nuclear localization sequence (NLS); this region, NLS2, is necessary for metastasis suppression. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.035DOI Listing
January 2019

Pre-structured hydrophobic peptide β-strands: A universal amyloid trap?

Arch Biochem Biophys 2019 Jan 29;664:51-61. Epub 2019 Jan 29.

Amyloid fibril formation has long been studied because of the variety of proteins that are capable of adopting this structure despite sharing little sequence homology. This makes amyloid fibrils a challenging focus for inhibition studies because the peptides and proteins that form amyloid fibrils cannot be targeted based on a sequence motif. Most peptide inhibitors that target specific amyloidogenic proteins rely heavily on sequence recognition to ensure that the inhibitory peptide is able to bind its target. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.032DOI Listing
January 2019

Cyanobacterial peptides as a tour de force in the chemical space of antiparasitic agents.

Arch Biochem Biophys 2019 Jan 29;664:24-39. Epub 2019 Jan 29.

Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Campus Curauma, Curauma, Valparaíso, Chile. Electronic address:

Parasites are scarcely addressed target for antimicrobial peptides despite their big impact in health and global economy. The notion of antimicrobial peptides is frequently associated to the innate immune defense of vertebrates and invertebrate vectors, as the ultimate recipients of the parasite infection. These antiparasite peptides are produced by ribosomal synthesis, with few post-translational modifications, and their diversity come mostly from their amino acid sequence. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183091
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http://dx.doi.org/10.1016/j.abb.2019.01.030DOI Listing
January 2019
3 Reads
3.017 Impact Factor

Structural and dynamical thermostability of psychrophilic enzyme at various temperatures: Molecular dynamics simulations of tryptophan synthase.

Arch Biochem Biophys 2019 Mar 28;663:297-305. Epub 2019 Jan 28.

Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad, 45600, Pakistan. Electronic address:

Mesophilic enzymes are among the most frequently used biocatalysts, however, psychrophilic enzymes are crucially important for their use in heat-sensitive reactions. How enzymes can work efficiently at various range of temperatures is an interesting subject for researchers, and yet it is very least explored. The structural and dynamical behavior of psychrophilic enzymes and their thermostability at various temperatures can help to understand the mechanism and function at molecular level, and for this purpose the ligand-free α-subunit of Shewanella frigidimarina's tryptophan synthase (Sf-TRPS) in isolated monomeric and in hetero-αβ-dimeric states was subjected to molecular dynamics (MD) simulations study. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.022DOI Listing

PvdF of pyoverdin biosynthesis is a structurally unique N-formyltetrahydrofolate-dependent formyltransferase.

Arch Biochem Biophys 2019 Jan 26;664:40-50. Epub 2019 Jan 26.

Department of Molecular Biosciences, 1200 Sunnyside Ave, University of Kansas, Lawrence, KS, 66045, USA. Electronic address:

The hydroxyornithine transformylase from Pseudomonas aeruginosa is known by the gene name pvdF, and has been hypothesized to use N-formyltetrahydrofolate (N-fTHF) as a co-substrate formyl donor to convert N-hydroxyornithine (OHOrn) to N-formyl- N-hydroxyornithine (fOHOrn). PvdF is in the biosynthetic pathway for pyoverdin biosynthesis, a siderophore generated under iron-limiting conditions that has been linked to virulence, quorum sensing and biofilm formation. The structure of PvdF was determined by X-ray crystallography to 2. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.028DOI Listing
January 2019

SR-mitochondria communication in adult cardiomyocytes: A close relationship where the Ca has a lot to say.

Arch Biochem Biophys 2019 Mar 24;663:259-268. Epub 2019 Jan 24.

Center for Translational Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, PA, 19107, USA. Electronic address:

In adult cardiomyocytes, T-tubules, junctional sarcoplasmic reticulum (jSR), and mitochondria juxtapose each other and form a unique and highly repetitive functional structure along the cell. The close apposition between jSR and mitochondria creates high Ca microdomains at the contact sites, increasing the efficiency of the excitation-contraction-bioenergetics coupling, where the Ca transfer from SR to mitochondria plays a critical role. The SR-mitochondria contacts are established through protein tethers, with mitofusin 2 the most studied SR-mitochondrial "bridge", albeit controversial. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.026DOI Listing
March 2019
1 Read

Troponin I modulation of cardiac performance: Plasticity in the survival switch.

Arch Biochem Biophys 2019 Jan 23;664:9-14. Epub 2019 Jan 23.

Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, 48109, USA. Electronic address:

Signaling complexes targeting the myofilament are essential in modulating cardiac performance. A central target of this signaling is cardiac troponin I (cTnI) phosphorylation. This review focuses on cTnI phosphorylation as a model for myofilament signaling, discussing key gaps and future directions towards understanding complex myofilament modulation of cardiac performance. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.025DOI Listing
January 2019
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Role of mitochondrial Ca homeostasis in cardiac muscles.

Arch Biochem Biophys 2019 Mar 23;663:276-287. Epub 2019 Jan 23.

Lillehei Heart Institute, Department of Medicine, Cardiovascular Division, University of Minnesota, Minneapolis, MN, USA. Electronic address:

Recent discoveries of the molecular identity of mitochondrial Ca influx/efflux mechanisms have placed mitochondrial Ca transport at center stage in views of cellular regulation in various cell-types/tissues. Indeed, mitochondria in cardiac muscles also possess the molecular components for efficient uptake and extraction of Ca. Over the last several years, multiple groups have taken advantage of newly available molecular information about these proteins and applied genetic tools to delineate the precise mechanisms for mitochondrial Ca handling in cardiomyocytes and its contribution to excitation-contraction/metabolism coupling in the heart. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.027DOI Listing
March 2019
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Isoniazid induces a monocytic-like phenotype in HL-60 cells.

Arch Biochem Biophys 2019 Jan 22;664:15-23. Epub 2019 Jan 22.

Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada. Electronic address:

Isoniazid (INH) is one of the oldest drugs for the treatment of tuberculosis (TB) and is of continual clinical and research interest. The aim of the current study is to investigate the ability of INH to induce monocyte differentiation and the underlying signaling pathway involved in this phenomenon using HL-60 cells. In this study, HL-60 cells were treated with different non-cytotoxic concentrations of INH or vitamin D (a well-known inducer of monocytic differentiation) to determine key functional changes in the phenotype of these cells using several biochemical and cytobiological experiments. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.004DOI Listing
January 2019
2 Reads
3.017 Impact Factor

Dysregulated mitochondrial Ca and ROS signaling in skeletal muscle of ALS mouse model.

Arch Biochem Biophys 2019 Mar 22;663:249-258. Epub 2019 Jan 22.

Kansas City University of Medicine and Bioscience, Kansas City, MO 64106, USA; College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, TX 76019, USA. Electronic address:

Amyotrophic lateral sclerosis (ALS) is a devastating neuromuscular disease characterized by motor neuron loss and prominent skeletal muscle wasting. Despite more than one hundred years of research efforts, the pathogenic mechanisms underlying neuromuscular degeneration in ALS remain elusive. While the death of motor neuron is a defining hallmark of ALS, accumulated evidences suggested that in addition to being a victim of motor neuron axonal withdrawal, the intrinsic skeletal muscle degeneration may also actively contribute to ALS disease pathogenesis and progression. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183077
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http://dx.doi.org/10.1016/j.abb.2019.01.024DOI Listing
March 2019
3 Reads

Human airway trypsin-like protease enhances interleukin-8 synthesis in bronchial epithelial cells by activating protease-activated receptor 2.

Arch Biochem Biophys 2019 Jan 21. Epub 2019 Jan 21.

Graduate School of Health Care Sciences, Jikei Institute, Osaka, Japan.

Human airway trypsin-like protease (HAT) localizes at human bronchial epithelial cells (HBECs). HAT enhanced release of interleukin-8 (IL-8) from HBECs at 10-100 mU/mL and the enhanced release was almost completely abolished by 50 μM leupeptin, a serine protease inhibitor. Previous reports suggested that HAT displays its physiological functions via protease-activated receptor 2 (PAR2). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183059
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http://dx.doi.org/10.1016/j.abb.2019.01.019DOI Listing
January 2019
5 Reads

MicroRNA-31 triggers G/M cell cycle arrest, enhances the chemosensitivity and inhibits migration and invasion of human gastric cancer cells by downregulating the expression of zeste homolog 2 (ZH2).

Arch Biochem Biophys 2019 Mar 21;663:269-275. Epub 2019 Jan 21.

Clinical Medical College of Jiangsu University, Department of Gastrointestinal Surgery, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, 215300, China. Electronic address:

Gastric cancer is the second most leading cause of cancer related mortality across the world over. Although the incidence of GC has declined to some extent but it is still the fourth highly diagnosed cancer across the world. GC generally remains undiagnosed till advanced stages due to unavailability of biomarkers and when diagnosed it becomes difficult to manage due to the lack of therapeutic targets and efficient chemotherapy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183095
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http://dx.doi.org/10.1016/j.abb.2019.01.023DOI Listing
March 2019
2 Reads

Distinct mechanisms of substrate selectivity in the DRE-TIM metallolyase superfamily: A role for the LeuA dimer regulatory domain.

Arch Biochem Biophys 2019 Jan 19;664:1-8. Epub 2019 Jan 19.

Department of Chemistry & Biochemistry, The University of Alabama, Tuscaloosa, AL, 35487, United States. Electronic address:

The use of modular domains in proteins affords nature a simple route to the diversification of protein function, but co-evolution between domains can complicate large-scale functional annotation. The LeuA dimer regulatory domain is primarily responsible for allosteric feedback inhibition of the enzymes isopropylmalate synthase (IPMS) and citramalate synthase (CMS). In addition to this regulatory role, presence of the domain may also affect substrate selectivity in certain members of the enzyme family. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.021DOI Listing
January 2019
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Hyaluronan fragments produced during tissue injury: A signal amplifying the inflammatory response.

Arch Biochem Biophys 2019 Mar 19;663:228-238. Epub 2019 Jan 19.

Department of Clinical and Experimental Medicine, University of Messina, Policlinico Universitario, 98125, Messina, Italy. Electronic address:

Inflammation is a complex mechanism that plays a key role during diseases. Dynamic features of the extracellular matrix (ECM), in particular, during phases of tissue inflammation, have long been appreciated, and a great deal of several investigations has focused on the effects of ECM derivatives on cell function. It has been well defined that during inflammatory and tissue injury, ECM components were degraded. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.015DOI Listing

Mitochondrial dynamics and inter-mitochondrial communication in the heart.

Arch Biochem Biophys 2019 Mar 18;663:214-219. Epub 2019 Jan 18.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, 100191, China; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China. Electronic address:

Mitochondria are organelles highly dynamic in most types of cells, constantly changing morphology and forming dynamically continuous networks. Defects of mitochondrial dynamics are associated with various human diseases including neurodegenerative diseases and cardiovascular diseases. In the heart, mitochondria are rigidly organized between myofilaments into a crystal-like lattice pattern, the apparently limited mitochondrial movement raises the question of whether mitochondria communicate with each other dynamically. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.017DOI Listing
March 2019
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β-hydroxybutyrate protects hepatocytes against endoplasmic reticulum stress in a sirtuin 1-independent manner.

Arch Biochem Biophys 2019 Mar 18;663:220-227. Epub 2019 Jan 18.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.

β-hydroxybutyrate (BHB), a major ketone body in mammals, is produced from fatty acids through mitochondrial fatty acid oxidation in hepatocytes. To elucidate the role of BHB in the hepatic endoplasmic reticulum (ER), we examined the effects of BHB on hepatic ER stress induced by tunicamycin. In mouse hepatoma Hepa1c1c7 cells, BHB treatment suppressed the protein expression of ER stress responsive genes and increased cell viability, while reducing the protein expression of apoptosis inducible genes, without causing any alterations in the protein expression of sirtuin 1 (SIRT1) or the phosphorylation of AMP-activated protein kinase. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.020DOI Listing
March 2019
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Time-lapse imaging of Ca-induced swelling and permeability transition: Single mitochondrion study.

Arch Biochem Biophys 2019 Mar 17;663:288-296. Epub 2019 Jan 17.

Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo, 184-8588, Japan. Electronic address:

Mitochondrial functions are closely related to the membrane structure. Mitochondrial swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon swelling remain unknown. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.016DOI Listing
March 2019
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Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3.

Arch Biochem Biophys 2019 Mar 16;663:239-248. Epub 2019 Jan 16.

Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, K1H 8M5, Canada. Electronic address:

Mitochondrial reactive oxygen species (ROS) are important cellular signaling molecules, but can cause oxidative damage if not kept within tolerable limits. An important proximal form of ROS in mitochondria is superoxide. Its production is thought to occur in regulated stochastic bursts, but current methods using mitochondrial targeted cpYFP to assess superoxide flashes are confounded by changes in pH. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.018DOI Listing
March 2019
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Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

Arch Biochem Biophys 2019 Mar 16;663:192-198. Epub 2019 Jan 16.

Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901, Ribeirão Preto, São Paulo, Brazil. Electronic address:

Tissue-nonspecific alkaline phosphatase (TNAP) is a key enzyme in the biomineralization process as it produces phosphate from a number of phospho-substrates stimulating mineralization while it also inactivates inorganic pyrophosphate, a potent mineralization inhibitor. We have previously reported on the reconstitution of TNAP on Langmuir monolayers as well as proteoliposomes. In the present study, thin films composed of dimyristoylphosphatidic acid (DMPA) were deposited on titanium supports by the Langmuir-Blodgett (LB) technique, and we determined preservation of TNAP's phosphohydrolytic activity after incorporation into the LB films. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.014DOI Listing
March 2019
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The human DNA topoisomerase I mutant Gly717Asp: Higher religation rate is not always associated with camptothecin resistance.

Arch Biochem Biophys 2019 Mar 14;663:165-172. Epub 2019 Jan 14.

Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133, Rome, Italy; Institute of Translational Pharmacology, National Research Council, CNR, Via Del Fosso del Cavaliere 100, Rome, 00133, Italy. Electronic address:

DNA topoisomerases are key enzyme responsible for modulating the topological state of the DNA by breaking and rejoining of DNA strand. Characterization of a Gly717Asp mutation in the human topoisomerase was performed using several catalytic assays. The mutant enzyme was shown to have comparable cleavage and fast religation rate as compared to the wild-type protein. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.007DOI Listing
March 2019
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Information theoretic measures and mutagenesis identify a novel linchpin residue involved in substrate selection within the nucleotide-binding domain of an ABCG family exporter Cdr1p.

Arch Biochem Biophys 2019 Mar 14;663:143-150. Epub 2019 Jan 14.

Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Gurgaon, India. Electronic address:

ABC transporters are membrane-bound pumps composed of two major domains: the transmembrane domain(s) (TMDs) and the nucleotide-binding domain(s) (NBDs). Sequence analyses of the NBDs of key ABC exporters revealed a residue position within the H-loop to be differentially conserved in the ABCG family, wherein there lies glutamine instead of positively charged arginine/lysine as in non-ABCG members. Consequently, contrasting NBD sequences of fungal Pleiotropic Drug Resistance transporters (PDR/ABCG) with that of Cholesterol/Phospholipid and Retinal (CPR/ABCA) Flippase family revealed a high Cumulative Relative Entropy (CRE) score of this residue position implying its family-specific functional significance. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.013DOI Listing
March 2019
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Characterization and mutation anaylsis of a cold-active bacterial hormone-sensitive lipase from Salinisphaera sp. P7-4.

Arch Biochem Biophys 2019 Mar 14;663:132-142. Epub 2019 Jan 14.

Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul, 04310, Republic of Korea. Electronic address:

In mammals, hormone sensitive lipase (EC 3.1.1. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.010DOI Listing
March 2019
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Molecular and cellular regulation of human glucokinase.

Arch Biochem Biophys 2019 Mar 11;663:199-213. Epub 2019 Jan 11.

Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, 32306, USA. Electronic address:

Glucose metabolism in humans is tightly controlled by the activity of glucokinase (GCK). GCK is predominantly produced in the pancreas, where it catalyzes the rate-limiting step of insulin secretion, and in the liver, where it participates in glycogen synthesis. A multitude of disease-causing mutations within the gck gene have been identified. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.011DOI Listing
March 2019
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Application of immobilized ATP to the study of NLRP inflammasomes.

Arch Biochem Biophys 2019 Jan 11. Epub 2019 Jan 11.

Department of Biochemistry & Molecular Biology, University of Calgary, 3280 Hospital Drive NW, Calgary, AB, T2N 4Z6, Canada. Electronic address:

The NLRP proteins are a subfamily of the NOD-like receptor (NLR) innate immune sensors that possess an ATP-binding NACHT domain. As the most well studied member, NLRP3 can initiate the assembly process of a multiprotein complex, termed the inflammasome, upon detection of a wide range of microbial products and endogenous danger signals and results in the activation of pro-caspase-1, a cysteine protease that regulates multiple host defense pathways including cytokine maturation. Dysregulated NLRP3 activation contributes to inflammation and the pathogenesis of several chronic diseases, and the ATP-binding properties of NLRPs are thought to be critical for inflammasome activation. Read More

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http://dx.doi.org/10.1016/j.abb.2018.12.031DOI Listing
January 2019
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A physiological concentration of luteolin induces phase II drug-metabolizing enzymes through the ERK1/2 signaling pathway in HepG2 cells.

Arch Biochem Biophys 2019 Mar 11;663:151-159. Epub 2019 Jan 11.

Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan. Electronic address:

The flavon luteolin has various health-promoting activities including cardiovascular protection, anti-inflammatory activity and anticancer activity. A serum concentration of about 100 nM luteolin is reached by dietary habit. However, little is known about the function of luteolin over its physiological concentration range. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.012DOI Listing
March 2019
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Identification of potential target genes associated with the reversion of androgen-dependent skeletal muscle atrophy.

Arch Biochem Biophys 2019 Mar 11;663:173-182. Epub 2019 Jan 11.

Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Brazil. Electronic address:

Muscle wasting or atrophy is extensively associated with human systemic diseases including diabetes, cancer, and kidney failure. Accumulating evidence from transcriptional profiles has noted that a common set of genes, termed atrogenes, is modulated in atrophying muscles. However, the transcriptional changes that trigger the reversion or attenuation of muscle atrophy have not been characterized at the molecular level until now. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.009DOI Listing
March 2019
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Regulating myofilament power: The determinant of health.

Arch Biochem Biophys 2019 Mar 10;663:160-164. Epub 2019 Jan 10.

Department of Medical Pharmacology & Physiology, School of Medicine, University of Missouri, Columbia, MO, 65212, USA. Electronic address:

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http://dx.doi.org/10.1016/j.abb.2019.01.008DOI Listing
March 2019
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LncRNA CACS15 contributes to oxaliplatin resistance in colorectal cancer by positively regulating ABCC1 through sponging miR-145.

Arch Biochem Biophys 2019 Mar 9;663:183-191. Epub 2019 Jan 9.

Department of Interventional Radiology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China. Electronic address:

Increasing evidence suggests that long non-coding RNAs (lncRNAs) are implicated with chemoresistance of cancers. However, their functional role and molecular mechanisms in colorectal cancer (CRC) chemoresistance are still largely unclear. In this work, we aimed to investigate the functional role of lncRNA cancer susceptibility candidate 15 (CASC15) in oxaliplatin (OXA) resistance of CRC and reveal the underlying molecular mechanism. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183091
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http://dx.doi.org/10.1016/j.abb.2019.01.005DOI Listing
March 2019
4 Reads

Closing the therapeutic loop.

Arch Biochem Biophys 2019 Mar 9;663:129-131. Epub 2019 Jan 9.

Department of Mechanical Engineering and Department of Surgery, University of Kentucky, United States.

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http://dx.doi.org/10.1016/j.abb.2019.01.006DOI Listing
March 2019
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Vitamin C and vitamin E double-deficiency increased neuroinflammation and impaired conditioned fear memory.

Arch Biochem Biophys 2019 Mar 8;663:120-128. Epub 2019 Jan 8.

Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, Japan. Electronic address:

Background: Vitamin C (l-ascorbic acid, VC) and vitamin E (α-tocopherol, VE) play important physiological roles as endogenous antioxidants in many tissues and organs. However, their roles in the brain remain entirely elusive. We established senescence marker protein 30 (SMP30)/α-tocopherol transfer protein (αTTP) double knockout (DKO) mice as a novel VC and VE double-deficiency model and examined the effect of VC and VE double-deficiency on brain functions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183084
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http://dx.doi.org/10.1016/j.abb.2019.01.003DOI Listing
March 2019
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Mitochondrial regulation of airway smooth muscle functions in health and pulmonary diseases.

Arch Biochem Biophys 2019 Mar 8;663:109-119. Epub 2019 Jan 8.

Center for Translational Medicine, Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA. Electronic address:

Mitochondria are important for airway smooth muscle physiology due to their diverse yet interconnected roles in calcium handling, redox regulation, and cellular bioenergetics. Increasing evidence indicates that mitochondria dysfunction is intimately associated with airway diseases such as asthma, IPF and COPD. In these pathological conditions, increased mitochondrial ROS, altered bioenergetics profiles, and calcium mishandling contribute collectively to changes in cellular signaling, gene expression, and ultimately changes in airway smooth muscle contractile/proliferative properties. Read More

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http://dx.doi.org/10.1016/j.abb.2019.01.002DOI Listing
March 2019
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Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18.

Arch Biochem Biophys 2019 Mar 4;663:71-82. Epub 2019 Jan 4.

Department of Biochemistry, Shimane University School of Medicine, Izumo, 693-8501, Japan; mAbProtein Co. Ltd, Izumo, 693-8501, Japan. Electronic address:

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that evokes both innate and acquired immune responses. IL-18 is initially synthesized as an inactive precursor and the cleavage for processing into a mature, active molecule is mediated by pro-inflammatory caspases following the activation of inflammasomes. Two types of monoclonal antibodies were raised: anti-IL-18 antibodies which recognize full-length and cleaved IL-18; and anti-IL-18 neoepitope antibodies which specifically recognize the new N-terminal YFGKLESK of IL-18 cleaved by pro-inflammatory caspase-1/4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183101
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http://dx.doi.org/10.1016/j.abb.2019.01.001DOI Listing
March 2019
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Glycoreplica peptides to investigate molecular mechanisms of immune-mediated physiological versus pathological conditions.

Arch Biochem Biophys 2019 Mar 27;663:44-53. Epub 2018 Dec 27.

Laboratory of Peptide and Protein Chemistry and Biology, Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 13, 50019, Sesto Fiorentino, Italy; Platform of Peptide and Protein Chemistry and Biology - PeptLab@UCP and Laboratory of Chemical Biology EA4505, Université Paris-Seine, 5 Mail Gay-Lussac, 95031, Cergy-Pontoise CEDEX, France. Electronic address:

Investigation of the role of saccharides and glycoconjugates in mechanisms of immune-mediated physiological and pathological conditions is a hot topic. In fact, in many autoimmune diseases cross-reactivity between sugar moieties exposed on exogenous pathogens and self-molecules has long been hinted. Several peptides have been reported as mimetics of glycans specifically interacting with sugar-binding antibodies. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183091
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http://dx.doi.org/10.1016/j.abb.2018.12.030DOI Listing
March 2019
2 Reads

Interactions of polyunsaturated fatty acids with amyloid peptides Aβ40 and Aβ42.

Arch Biochem Biophys 2019 Mar 26;663:34-43. Epub 2018 Dec 26.

School of Pharmacy, Health Sciences Campus, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. Electronic address:

Polyunsaturated fatty acids (PUFAs) are reported to exert beneficial effects in Alzheimer's disease. Some PUFAs are known to reduce amyloid-beta (Aβ) toxicity by promoting its degradation and clearance. Studies on the direct interactions of PUFAs with Aβ peptides are limited and contradictory. Read More

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http://dx.doi.org/10.1016/j.abb.2018.12.027DOI Listing
March 2019
1 Read