29,160 results match your criteria Archives of Biochemistry and Biophysics[Journal]


Inhibition of the signaling pathway of syndecan-1 by synstatin: A promising anti-integrin inhibitor of angiogenesis and proliferation in HCC in rats.

Arch Biochem Biophys 2018 Jun 18. Epub 2018 Jun 18.

Dept. of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt. Electronic address:

Aim Of Work: The study was conducted for evaluation of the antitumor activity of SSTN against HCC induced by thioacetamide in rats.

Methods: Sixty male Sprague-Dawley rats were randomized into four equal groups: Control, SSTN, HCC, and HCC + SSTN. Liver function tests were measured in serum. Read More

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June 2018
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Characterization of butyrylcholinesterase from porcine milk.

Arch Biochem Biophys 2018 Jun 14. Epub 2018 Jun 14.

Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA. Electronic address:

Human butyrylcholinesterase (HuBChE) is under development for use as a pretreatment antidote against nerve agent toxicity. Animals are used to evaluate the efficacy of HuBChE for protection against organophosphorus nerve agents. Pharmacokinetic studies of HuBChE in minipigs showed a mean residence time of 267 h, similar to the half-life of HuBChE in humans, suggesting a high degree of similarity between BChE from 2 sources. Read More

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Interaction of pirenzepine with bovine serum albumin and effect of β-cyclodextrin on binding: A biophysical and molecular docking approach.

Arch Biochem Biophys 2018 Jun 14;652:27-37. Epub 2018 Jun 14.

Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P, 202002, India. Electronic address:

Studying the interaction of therapeutic molecules with serum albumin is important to understand their biopharmaceutics, pharmacokinetics and toxicity as well as their relation with the structure and function of protein. The biomolecular interaction of an anti-spasmodic drug, pirenzepine with bovine serum albumin (BSA) was investigated using multi-spectroscopic, calorimetric and docking studies. Fluorescence quenching of BSA on interaction with pirenzepine revealed the static mode of quenching. Read More

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Designing a new multifunctional peptide for metal chelation and Aβ inhibition.

Arch Biochem Biophys 2018 Jun 12. Epub 2018 Jun 12.

Sharif University of Technology, Chemistry Department, Iran.

According to the Amyloid hypothesis, as the foremost scientific explanation for Alzheimer Disease (AD), the neuropathology of AD is related to toxic fragments of amyloid beta (Aβ) protein. Based on this hypothesis, an attractive therapeutic approach was demonstrated to identify multifunctional peptides able to modulate Aβ pathologies as the source of AD. On this premise, a bifunctional polypeptide based on the iAβ5p lead compound, was designed to inhibit Aβ aggregation and free metal ions. Read More

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Polyethyleneimine renders mitochondrial membranes permeable by interacting with negatively charged phospholipids in them.

Arch Biochem Biophys 2018 Jun 8;652:9-17. Epub 2018 Jun 8.

Institute for Genome Research, Tokushima University, Kuramotocho-3, Tokushima 770-8503, Japan; Faculty of Pharmaceutical Sciences, Tokushima University, Shomachi-1, Tokushima 770-8505, Japan.

Polyethyleneimines (PEIs) are used for transfection of cells with nucleic acids. Meanwhile, the interaction of PEI with mitochondria causes cytochrome c release prior to apoptosis; the mechanisms how PEI causes this permeabilization of mitochondrial membranes and the release of cytochrome c remain unclear. To clarify these mechanisms, we examined the effects of branched-type PEI and linear-type PEI, each of which was 25 kDa in size, on mitochondria. Read More

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Carotenoids in human nutrition and health.

Arch Biochem Biophys 2018 Jun 6;652:18-26. Epub 2018 Jun 6.

DSM Nutritional Products, R&D Human Nutrition, Wurmisweg 576, Kaiseraugst, Switzerland. Electronic address:

Carotenoids are naturally occurring pigments found in most fruits and vegetables, plants, algae, and photosynthetic bacteria. Humans cannot synthesize carotenoids and must ingest them in food or via supplementation. Carotenoids have a range of functions in human health. Read More

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Significance of H461 at subsite +1 in substrate binding and transglucosylation activity of amylomaltase from Corynebacterium glutamicum.

Arch Biochem Biophys 2018 Jun 6;652:3-8. Epub 2018 Jun 6.

Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:

Amylomaltase (AM) catalyzes inter- and intra-molecular transglycosylation reactions of glucan to yield linear and cyclic oligosaccharide products. The functional roles of the conserved histidine at position 461 in the active site of AM from Corynebacterium glutamicum (CgAM) was investigated. H461 A/S/D/R/W were constructed, their catalytic properties were compared to the wild-type (WT). Read More

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Introduction to special issue on Polyphenols and Health.

Arch Biochem Biophys 2018 May 31;652:1-2. Epub 2018 May 31.

Department of Clinical and Experimental Medicine, Faculty of Health and Medical Sciences, University of Surrey, UK.

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Phenolic metabolites in plasma and tissues of rats fed with a grape pomace extract as assessed by liquid chromatography-tandem mass spectrometry.

Arch Biochem Biophys 2018 Aug 31;651:28-33. Epub 2018 May 31.

Laboratorio de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza (IBAM), Facultad de Ciencias Agrarias, CONICET-Universidad Nacional de Cuyo, M5528AHB Chacras de Coria, Argentina.

Grape pomace extract (GPE) is a rich and relatively low-cost source of phenolic compounds. However, little is known about the main GPE metabolites in mammals, which could help explain the observed health-promoting effects. This study investigated the presence of parent compounds from flavanol, flavonol and stilbene families and their metabolites in rat plasma and tissues after an acute intake of GPE in doses of 300 and 600 mg kg/body weight. Read More

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Interactive structural analysis of βTrCP1 and PER2 phosphoswitch binding through dynamics simulation assay.

Arch Biochem Biophys 2018 Aug 30;651:34-42. Epub 2018 May 30.

National Center for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address:

Circadian rhythm is rhythmic gene expression that is involved in various processes of life over a day and night cycle. The rhythmic sleep disorders arise due to misalignment of sleep-wake cycle influenced by phosphorylation of PERIOD2 (PER2) phosphodegron (SSGYGS), the conserved interaction site of β-transducin repeat-containing protein (βTrCP1). Here, we employed in silico approach to study the interaction pattern of βTrCP1 with PER2, PER2 and PER2 phosphodegron peptides. Read More

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Dissection of the deep-blue autofluorescence changes accompanying amyloid fibrillation.

Arch Biochem Biophys 2018 Aug 24;651:13-20. Epub 2018 May 24.

Faculty of Physics, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119991, Russia. Electronic address:

Pathogenesis of numerous diseases is associated with the formation of amyloid fibrils. Extrinsic fluorescent dyes, including Thioflavin T (ThT), are used to follow the fibrillation kinetics. It has recently been reported that the so-called deep-blue autofluorescence (dbAF) is changing during the aggregation process. Read More

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microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2.

Arch Biochem Biophys 2018 Aug 23;651:52-60. Epub 2018 May 23.

Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing, PR China. Electronic address:

Adriamycin resistance is closely related to therapeutic efficacy in breast cancer patients and their prognosis. Increasing evidence has suggested that miRNA functions in Adriamycin resistance in various types of cancer. microRNA-129-5p (miR-129-5p) has been considered a tumor-suppressive miRNA in several cancers, but its potential role in Adriamycin resistance in breast cancer has not been fully elucidate. Read More

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August 2018
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Plasma urolithin metabolites correlate with improvements in endothelial function after red raspberry consumption: A double-blind randomized controlled trial.

Arch Biochem Biophys 2018 Aug 24;651:43-51. Epub 2018 May 24.

Department of Nutritional Sciences, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, UK; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University Düsseldorf, Düsseldorf, Germany. Electronic address:

Raspberries are a rich source of ellagitannins and anthocyanins. The aim of this work was to investigate whether raspberry consumption can improve vascular function and to understand which phenolic metabolites may be responsible for the effects. A 3 arm double-blind randomized controlled crossover human intervention trial was conducted in 10 healthy males. Read More

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Modification of chickpea cystatin by reactive dicarbonyl species: Glycation, oxidation and aggregation.

Arch Biochem Biophys 2018 Jul 26;650:103-115. Epub 2018 May 26.

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India. Electronic address:

Reactive dicarbonyl species such as methylglyoxal (MGO) and glyoxal (GO) have recently received extensive attention due to their high reactivity and ability to modify biological substances such as proteins, phospholipids, and DNA. In case of proteins these reactive species mainly react with lysine and arginine residues to form AGEs, oxidative products, and aggregates. Chickpea cystatin (CPC) was incubated with varying concentrations of glyoxal and methylglyoxal which caused, along with altered secondary and tertiary structures, glycation, functional inactivation, altered redox state, cross-linking and high-molecular-mass aggregation. Read More

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July 2018
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Scp-2/Scp-x ablation in Fabp1 null mice differentially impacts hepatic endocannabinoid level depending on dietary fat.

Arch Biochem Biophys 2018 Jul 12;650:93-102. Epub 2018 May 12.

Department of Physiology and Pharmacology, Texas A&M University, College Station, TX 77843-4466, USA. Electronic address:

Dysregulation of the hepatic endocannabinoid (EC) system and high fat diet (HFD) are associated with non-alcoholic fatty liver disease. Liver cytosol contains high levels of two novel endocannabinoid binding proteins-liver fatty acid binding protein (FABP1) and sterol carrier protein-2 (SCP-2). While Fabp1 gene ablation significantly increases hepatic levels of arachidonic acid (ARA)-containing EC and sex-dependent response to pair-fed high fat diet (HFD), the presence of SCP-2 complicates interpretation. Read More

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A novel miR17/protein tyrosine phosphatase-oc/EphA4 regulatory axis of osteoclast activity.

Arch Biochem Biophys 2018 Jul 17;650:30-38. Epub 2018 May 17.

Musculoskeletal Disease Center, Jerry L. Pettis Memorial VA Medical Center, Loma Linda, CA 92357, USA; Department of Medicine, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.

Information about the molecular mechanisms leading to the activation of the osteoclast is relatively limited. While there is compelling evidence that the signaling mechanisms of Src and integrin β are essential for osteoclast activation, the regulation of these two signaling mechanisms is not fully understood. In this review, evidence supporting a novel regulatory axis of osteoclast activation that plays an upstream regulatory role in both the Src and integrin β signaling during osteoclast activation is discussed. Read More

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Mounting evidence validates Ursolic Acid directly activates SIRT1: A powerful STAC which mimic endogenous activator of SIRT1.

Arch Biochem Biophys 2018 Jul 17;650:39-48. Epub 2018 May 17.

Department of Biochemistry, Faculty of Basic Sciences, Islamic Azad University, Sanandaj Barnch, Sanandaj, Iran.

Ursolic Acid (UA), a pentacyclic triterpenoid compound, plays a vital role in aging process. However, the role of UA in the regulation of aging and longevity is still controversial as we have previously demonstrated that UA increases SIRT1 protein level in aged-mice. Here, we reveal that UA directly activates SIRT1 in silico, in vitro and in vivo. Read More

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July 2018
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Dietary vitamin A impacts DNA methylation patterns of adipogenesis-related genes in suckling rats.

Arch Biochem Biophys 2018 Jul 11;650:75-84. Epub 2018 May 11.

Grup de Recerca Nutrigenòmica i Obesitat, Laboratori de Biologia Molecular, Nutrició i Biotecnologia (LBNB), Universitat de les Illes Balears, Palma de Mallorca, Spain; CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain; Institut d'Investigació Sanitària Illes Balears (IdISBa), Palma de Mallorca, Spain.

We previously showed that vitamin A supplementation in early life impacts white adipose tissue (WAT) biology. We here studied the vitamin's effects on DNA methylation of genes crucial for WAT cell development, determination and metabolism. CpG promoter methylation and mRNA expression of Pparg, Zfp423, Pcna, and Rbp4 was compared in inguinal WAT of 21-day-old rats supplemented during the suckling period with vehicle (controls) or an emulsion of vitamin A as retinyl ester (RE) or β-carotene (BC). Read More

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MicroRNA-299-3p regulates proliferation, migration and invasion of human ovarian cancer cells by modulating the expression of OCT4.

Arch Biochem Biophys 2018 Aug 31;651:21-27. Epub 2018 May 31.

Department of Obstetrics and Gynecology, The Third People's Hospital of Jinan, Jinan, Shandong 250132, China.

Ovarian cancer is among the most prevalent and lethal types of cancers in women. Several factors such as late diagnosis, unavailability of the reliable biomarkers, frequent relapses and dearth of efficient therapeutic targets form bottleneck in the treatment of ovarian cancers. In this study we investigated the potential of less studied miR-299-3p as the therapeutic target for the treatment of ovarian cancer. Read More

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Ca signaling and Src-kinases-controlled cellular functions.

Arch Biochem Biophys 2018 Jul 16;650:59-74. Epub 2018 May 16.

Department of Cancer Biology, Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, c/ Arturo Duperier 4, E-28029 Madrid, Spain. Electronic address:

Calcium-mediated signaling and the functionality of Src-family tyrosine kinases (SFKs) are two interconnected processes. Activation of these kinases, which are coupled to a series of receptors, mediates Ca mobilization by regulating Ca channels, and the generated Ca signal in turn exerts control on the kinase activity via calmodulin. In this review, we shall cover the regulation of selected processes where crosstalk between the functionality of SFKs and the Ca signal occurs during the lifespan of the cell, when subjected to different extracellular or intracellular stimuli. Read More

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ID2 protects retinal pigment epithelium cells from oxidative damage through p-ERK1/2/ID2/NRF2.

Arch Biochem Biophys 2018 Jul 16;650:1-13. Epub 2018 May 16.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road, Guangzhou 510060, China; Biobank of Eye, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road, Guangzhou 510060, China. Electronic address:

Age-related macular degeneration (AMD) is the leading cause of blindness during aging. The degeneration of retinal pigment epithelium (RPE) is the main pathologic characteristic of AMD. ID2 is a member of the Inhibitor of DNA binding proteins (ID) family and is involved in regulation of cell proliferation and differentiation. Read More

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MS tools for a systematic approach in survey for carotenoids and their common metabolites.

Arch Biochem Biophys 2018 Jul 18;650:85-92. Epub 2018 May 18.

Laboratorio de Espectrometría de Masas, Instituto de la Grasa (CSIC), Sevilla, Spain.

Metabolism of the carotenoids in humans is often reserved to bioconversion of provitamin A carotenoids in retinoids and oxidative/degradative products arising from enzymatic eccentric cleavage, yielding apo-carotenoids. Nevertheless, additional metabolic routes should be available, and some of the structures of those metabolites have only been anticipated. The identification of carotenoids and their metabolites is commonly performed by HPLC coupled to MS. Read More

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Naringin protects against bone loss in steroid-treated inflammatory bowel disease in a rat model.

Arch Biochem Biophys 2018 Jul 17;650:22-29. Epub 2018 May 17.

Department of Emergency, Central Hospital of Zibo, Zibo, 255036, Shandong Province, China.

We observed the effects of naringin on bone loss in glucocorticoid-treated inflammatory bowel disease (IBD) in a rat model. The IBD model was established in Sprague-Dawley rats by administering 5.0% dextran sodium sulfate. Read More

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Wavelength-dependence of vasodilation and NO release from S-nitrosothiols and dinitrosyl iron complexes by far red/near infrared light.

Arch Biochem Biophys 2018 Jul 9;649:47-52. Epub 2018 May 9.

Department of Medicine- Division of Cardiovascular Medicine, United States; Cardiovascular Center, Medical College of Wisconsin, United States; Clement J Zablocki VA Medical Center, United States. Electronic address:

Far red/near infrared (R/NIR) energy is a novel therapy, but its mechanism of action is poorly characterized. Cytochrome c oxidase (Cco) of the mitochondrial electron transport chain is considered the primary photoacceptor for R/NIR to photolyze a putative heme nitrosyl in Cco to liberate free nitric oxide (NO). We previously observed R/NIR light directly liberates NO from nitrosylated hemoglobin and myoglobin, and recently suggested S-nitrosothiols (RSNO) and dinitrosyl iron complexes (DNIC) may be primary sources of R/NIR-mediated NO. Read More

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Excited-state properties of the central-cis isomer of the carotenoid peridinin.

Arch Biochem Biophys 2018 Jul 6;649:29-36. Epub 2018 May 6.

Photosynthetic Antenna Research Center, Washington University in St. Louis, St. Louis, MO, 63130, USA; Department of Biology, Washington University in St. Louis, St. Louis, MO, 63130, USA; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, 63130, USA.

The central-cis isomer of the carotenoid peridinin, presumably 13-cis, was separated and studied with spectroscopic methods including static absorption, fluorescence and femtosecond time-resolved absorption. The investigations exposed differences in the photophysical properties of this isomer in respect to all-trans peridinin. Steady-state absorption spectroscopy revealed the presence of an additional weak absorption band at the long wavelength tail of the main S → S transition. Read More

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Supplementation with macular carotenoids improves visual performance of transgenic mice.

Arch Biochem Biophys 2018 Jul 6;649:22-28. Epub 2018 May 6.

Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, USA. Electronic address:

Carotenoid supplementation can improve human visual performance, but there is still no validated rodent model to test their effects on visual function in laboratory animals. We recently showed that mice deficient in β-carotene oxygenase 2 (BCO2) and/or β-carotene oxygenase 1 (BCO1) enzymes can accumulate carotenoids in their retinas, allowing us to investigate the effects of carotenoids on the visual performance of mice. Using OptoMotry, a device to measure visual function in rodents, we examined the effect of zeaxanthin, lutein, and β-carotene on visual performance of various BCO knockout mice. Read More

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Testosterone alleviates mitochondrial ROS accumulation and mitochondria-mediated apoptosis in the gastric mucosa of orchiectomized rats.

Arch Biochem Biophys 2018 Jul 4;649:53-59. Epub 2018 May 4.

Hebei Institute of Physical Education, Shijiazhuang, PR China. Electronic address:

The effects of testosterone propionate (TP) on the oxidative stress and mitochondrial function, as well as on mitochondria associated apoptotic signaling, were analyzed in the gastric mucosa in orchiectomized male rats. The present study showed that testosterone deficiency triggered apoptosis by damaging the mitochondrial function (ROS overload generation, membrane potential loss, ATP depletion, etc.), increasing both the release of mitochondrial cytochrome c (Cyt c) and the Bax/Bcl-2 ratio, and activating caspase-9 and caspase-3. Read More

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Functional characterization of the NhaA Na/H antiporter from the green picoalga Ostreococcus tauri.

Arch Biochem Biophys 2018 Jul 4;649:37-46. Epub 2018 May 4.

Department of Microbiology, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand. Electronic address:

Transmembrane ion transport is a critical process in the cellular response to salt stress. Among the known functional membrane transporters that are involved in the salt stress response, Na/H antiporters have been extensively studied. These ubiquitous membrane proteins are crucial for salt tolerance and are associated with the regulation of internal pH, cell volume, morphogenesis, and vesicular trafficking. Read More

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Unified analysis of optical absorption spectra of carotenoids based on a stochastic model.

Arch Biochem Biophys 2018 Jul 4;650:49-58. Epub 2018 May 4.

School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan. Electronic address:

The chemical structures of the carotenoid molecules are very simple and one might think that their electronic features are easily predicted. However, there is still has so much unknown information excepting the correlation between the electronic energy state and the length of effective conjugation chain of carotenoids. To investigate the electronic feature of the carotenoids, the most essential method is measuring the optical absorption spectra, but simulations based on the resonance Raman spectra are also an effective approach. Read More

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Inhibition of PP2A by hesperetin may contribute to Akt and ERK1/2 activation status in cortical neurons.

Arch Biochem Biophys 2018 Jul 30;650:14-21. Epub 2018 Apr 30.

Hugh Sinclair Unit for Human Nutrition, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG2 6AP, UK.

Flavonoids and their metabolites are well reported to modulate the activation/phosphorylation of various cellular kinases, such as ERK1/2 and JNK, although the mechanism by which they do so is unclear. In this study, we investigated the impact of flavanones on the activation of PI3K/Akt and ERK1/2 and determine whether this is mediated, in part, by the inhibition of phosphatases. Primary cortical neurons were exposed to physiological concentrations of hesperetin and the phosphorylation status of the kinases PI3K/Akt and ERK1/2 and the phosphatases PP2A and PTEN were assessed by immunoblotting after 30 min. Read More

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Effects of human SULT1A3/SULT1A4 genetic polymorphisms on the sulfation of acetaminophen and opioid drugs by the cytosolic sulfotransferase SULT1A3.

Arch Biochem Biophys 2018 Jun 26;648:44-52. Epub 2018 Apr 26.

Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA. Electronic address:

Sulfoconjugation has been shown to be critically involved in the metabolism of acetaminophen (APAP), morphine, tapentadol and O-desmethyl tramadol (O-DMT). The objective of this study was to investigate the effects of single nucleotide polymorphisms (SNPs) of human SULT1A3 and SULT1A4 genes on the sulfating activity of SULT1A3 allozymes toward these analgesic compounds. Twelve non-synonymous coding SNPs (cSNPs) of SULT1A3/SULT1A4 were investigated, and the corresponding cDNAs were generated by site-directed mutagenesis. Read More

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BML-111 alleviates acute lung injury through regulating the expression of lncRNA MALAT1.

Arch Biochem Biophys 2018 Jul 25;649:15-21. Epub 2018 Apr 25.

Department of Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address:

BML-111 is a lipoxin receptor agonist that plays a vital role on inflammation. MALAT1 is reported to mediate lung injury. ALI rat model was established using the method of venous cannula. Read More

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Role of the C-terminus mobile domain of cardiac troponin I in the regulation of thin filament activation in skinned papillary muscle strips.

Arch Biochem Biophys 2018 Jun 25;648:27-35. Epub 2018 Apr 25.

The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA; The Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA 99164, USA. Electronic address:

The C-terminus mobile domain of cTnI (cTnI-MD) is a highly conserved region which stabilizes the actin-cTnI interaction during the diastole. Upon Ca-binding to cTnC, cTnI-MD participates in a regulatory switching that involves cTnI to switch from interacting with actin toward interacting with the Ca-regulatory domain of cTnC. Despite many studies targeting the cTnI-MD, the role of this region in the length-dependent activation of cardiac contractility is yet to be determined. Read More

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June 2018
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The potent insulin secretagogue effect of betulinic acid is mediated by potassium and chloride channels.

Arch Biochem Biophys 2018 Jun 25;648:20-26. Epub 2018 Apr 25.

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. Electronic address:

Betulinic acid (BA) has been described as an insulin secretagogue which may explain its potent antihyperglycemic effect; however, the exact role of BA as an insulinogenic agent is not clear. The aim of this study was to investigate the mechanism of BA on calcium influx and static insulin secretion in pancreatic islets isolated from euglycemic rats. We found that BA triggers calcium influx by a mechanism dependent on ATP-dependent potassium channels and L-type voltage-dependent calcium channels. Read More

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Circular RNA circMYO9B facilitates breast cancer cell proliferation and invasiveness via upregulating FOXP4 expression by sponging miR-4316.

Arch Biochem Biophys 2018 Apr 24. Epub 2018 Apr 24.

Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. Electronic address:

Recently, circular RNAs (circRNAs) have been demonstrated as essential regulators in human cancers. However, the function and mechanism of circRNAs in breast cancer (BC) remain largely unknown and require to be investigated. In the present study, we found that circMYO9B was highly expressed in BC tissues by bioinformatics analysis. Read More

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Alpha-synuclein mitochondrial interaction leads to irreversible translocation and complex I impairment.

Arch Biochem Biophys 2018 Aug 25;651:1-12. Epub 2018 Apr 25.

CONICET, Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular, Buenos Aires, Argentina. Electronic address:

α-synuclein is involved in both familial and sporadic Parkinson's disease. Although its interaction with mitochondria has been well documented, several aspects remains unknown or under debate such as the specific sub-mitochondrial localization or the dynamics of the interaction. It has been suggested that α-synuclein could only interact with ER-associated mitochondria. Read More

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Curcumin formulated in solid lipid nanoparticles has enhanced efficacy in Hodgkin's lymphoma in mice.

Arch Biochem Biophys 2018 Jun 18;648:12-19. Epub 2018 Apr 18.

Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA, 95616, USA; Department of Family, Population and Preventive Medicine, Stony Brook University, Stony Brook, NY, 11794-8175, USA. Electronic address:

Curcumin reduces Hodgkin's lymphoma (HL) cell growth in vitro, but its unfavorable pharmacokinetics highlight the need for novel in vivo delivery systems. Thus, we explored whether formulation of curcumin in solid lipid nanoparticles (SLN-curc) or d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS) nanoparticles (TPGS-curc) could enhance its efficacy in mice. Curcumin formulated in SLN and in TPGS resulted in higher curcumin plasma levels in mice. Read More

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June 2018
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Biological activities of laminin-111-derived peptide-chitosan matrices in a primary culture of rat cortical neurons.

Arch Biochem Biophys 2018 Jun 17;648:53-59. Epub 2018 Apr 17.

Department of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, 192-0392, Japan.

Cell adhesive biomaterials have been used for various cells in culture, especially for primary cultures of neurons. Here we examined laminin-111 and its active peptides conjugated to chitosan matrices (ChtMs) for primary culture of rat cortical neurons. Laminin-111 on poly-d-lysine substrate promoted neuronal cell attachment and differentiation. Read More

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June 2018
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Poria cocos polysaccharide attenuates RANKL-induced osteoclastogenesis by suppressing NFATc1 activity and phosphorylation of ERK and STAT3.

Arch Biochem Biophys 2018 Jun 17;647:76-83. Epub 2018 Apr 17.

School of Biomedical Sciences, University of Western Australia, Perth, WA, 6009, Australia. Electronic address:

Pathological fractures caused by osteolytic lesions seriously threaten the health of patients. Osteoclasts play important roles in bone resorption whose hyperfunction are closely related to osteolytic lesions. Studies on osteoclast differentiation and function assist in the prevention of excessive bone loss associated diseases. Read More

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June 2018
2 Reads

Structural and functional insights into RHA-P, a bacterial GH106 α-L-rhamnosidase from Novosphingobium sp. PP1Y.

Arch Biochem Biophys 2018 Jun 18;648:1-11. Epub 2018 Apr 18.

Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Via S. Allende 2, 84131, Salerno, Italy. Electronic address:

α-L-Rhamnosidases (α-RHAs, EC 3.2.1. Read More

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June 2018
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Potential role of MicroRNA 200c gene expression in assessment of colorectal cancer.

Arch Biochem Biophys 2018 Jun 14;647:41-46. Epub 2018 Apr 14.

Organic Chemistry Department, Faculty of Science, Menoufia University, Egypt. Electronic address:

Background And Aim: Colorectal cancer (CRC) is a common cancer worldwide that affects men and women of all racial and ethnic groups. Recent evidence supports the role of microRNAs in CRC. We planned to investigate microRNA200c expression and its relation with diagnosis, prognosis, metastasis and overall survival in CRC patients. Read More

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June 2018
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Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

Arch Biochem Biophys 2018 Jun 12;647:10-32. Epub 2018 Apr 12.

Institute of Bioinformatics and Structural Biology and Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:

With the concept of precision medicine, combining multiple molecular-targeting therapies has brought new approaches to current cancer treatments. Malfunction of the tumor suppressor protein, p53 is a universal hallmark in human cancers. Under normal conditions, p53 is degraded through an ubiquitin-proteosome pathway regulated by its negative regulator, MDM2. Read More

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June 2018
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Lutein, zeaxanthin and mammalian development: Metabolism, functions and implications for health.

Arch Biochem Biophys 2018 Jun 11;647:33-40. Epub 2018 Apr 11.

Department of Food Science; Rutgers Center for Lipid Research; New Jersey Institute for Food, Nutrition, and Health, Rutgers University, New Brunswick, NJ 08901, United States. Electronic address:

It is now widely accepted that nutrition during critical periods in early development, both pre- and postnatal, may have lifetime consequences in determining health or onset of major diseases in the adult life. Dietary carotenoids have shown beneficial health effects throughout the life cycle due to their potential antioxidant properties, their ability to serves as precursors of vitamin A and to the emerging signaling functions of their metabolites. The non-provitamin A carotenoids lutein and zeaxanthin are emerging as important modulators of infant and child visual and cognitive development, as well as critical effectors in the prevention and treatment of morbidity associated with premature births. Read More

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June 2018
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Carotenoid esters analysis and occurrence: What do we know so far?

Arch Biochem Biophys 2018 Jun 12;648:36-43. Epub 2018 Apr 12.

Department of Food Science, School of Food Engineering, University of Campinas, São Paulo, Brazil. Electronic address:

Carotenoids possessing hydroxyl groups can be found in nature both as free xanthophylls and as carotenoid esters, i.e. acylated with fatty acids. Read More

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June 2018
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Dominant and sensitive control of oxidative flux by the ATP-ADP carrier in human skeletal muscle mitochondria: Effect of lysine acetylation.

Arch Biochem Biophys 2018 Jun 10;647:93-103. Epub 2018 Apr 10.

University of Arizona, College of Medicine, Department of Medicine, 1501 N. Campbell Avenue, P.O. Box 245099, Tucson, AZ 85724-5099, USA. Electronic address:

The adenine nucleotide translocase (ANT) of the mitochondrial inner membrane exchanges ADP for ATP. Mitochondria were isolated from human vastus lateralis muscle (n = 9). Carboxyatractyloside titration of O consumption rate (J) at clamped [ADP] of 21 μM gave ANT abundance of 0. Read More

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June 2018
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Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis: Binding studies, site-directed mutagenesis and stereoselectivity.

Arch Biochem Biophys 2018 Jun 11;647:1-9. Epub 2018 Apr 11.

Laboratoire des Energies de Demain, LIED, UMR 8236 CNRS, Université Paris Diderot-Paris 7, bâtiment Lamarck B, boîte courier 7040, 35 rue Hélène Brion, 75205 Paris Cedex 13, France; Ecole Nationale Supérieure de Chimie de Paris, Chimie Paris-Tech, 11 rue P. et M. Curie, 75231 Paris Cedex 05, France. Electronic address:

Cylindrospermopsin, a cytotoxin from cyanobacteria, is biosynthesized by a complex pathway, which involves CyrI, an iron and 2-oxoglutarate dependent hydroxylase that transforms 7-deoxy-cylindrospermopsin into cylindrospermopsin and its epimer, 7-epi-cylindrospermopsin, in the last step. The activity of CyrI from Oscillatoria sp. PCC 7926 depends on Fe(II) (K = 2. Read More

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June 2018
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Effects of grape consumption on biomarkers of inflammation, endothelial function, and PBMC gene expression in obese subjects.

Arch Biochem Biophys 2018 May 9;646:145-152. Epub 2018 Apr 9.

Department of Nutrition and Graduate Group in Nutritional Biology, University of California, Davis, CA 95616, United States. Electronic address:

This study investigated effects of grape consumption on biomarkers of cardiovascular health in obese participants in both postprandial and chronic settings. Twenty obese adults participated in this randomized, placebo controlled, double-blinded crossover trial. Participants were randomized to consume 60 g freeze-dried polyphenol-rich whole grape powder (GP) or placebo (PBO) followed by high fat high carbohydrate (HFHC) meal challenge. Read More

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May 2018
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HCM and DCM cardiomyopathy-linked α-tropomyosin mutations influence off-state stability and crossbridge interaction on thin filaments.

Arch Biochem Biophys 2018 Jun 5;647:84-92. Epub 2018 Apr 5.

Department of Biological Sciences, University of Massachusetts-Lowell, One University Avenue, Lowell, MA 01854, USA. Electronic address:

Calcium regulation of cardiac muscle contraction is controlled by the thin-filament proteins troponin and tropomyosin bound to actin. In the absence of calcium, troponin-tropomyosin inhibits myosin-interactions on actin and induces muscle relaxation, whereas the addition of calcium relieves the inhibitory constraint to initiate contraction. Many mutations in thin filament proteins linked to cardiomyopathy appear to disrupt this regulatory switching. Read More

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June 2018
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Characterisation of peroxidasin activity in isolated extracellular matrix and direct detection of hypobromous acid formation.

Arch Biochem Biophys 2018 May 4;646:120-127. Epub 2018 Apr 4.

Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, Christchurch, New Zealand. Electronic address:

Peroxidasin is a heme peroxidase that catalyses the oxidation of bromide by hydrogen peroxide to form an essential sulfilimine cross-link between methionine and hydroxylysine residues in collagen IV. We investigated cross-linking by peroxidasin embedded in extracellular matrix isolated from cultured epithelial cells and its sensitivity to alternative substrates and peroxidase inhibitors. Peroxidasin showed peroxidase activity as measured with hydrogen peroxide and Amplex red. Read More

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May 2018
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Theophylline suppresses interleukin-6 expression by inhibiting glucocorticoid receptor signaling in pre-adipocytes.

Arch Biochem Biophys 2018 May 4;646:98-106. Epub 2018 Apr 4.

Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 6578501, Japan.

Adipose tissues in obese individuals are characterized by a state of chronic low-grade inflammation. Pre-adipocytes and adipocytes in this state secrete pro-inflammatory adipokines, such as interleukin 6 (IL-6), which induce insulin resistance and hyperglycemia. Theophylline (1,3-dimethylxanthine) exerts anti-inflammatory effects, but its effects on pro-inflammatory adipokine secretion by pre-adipocytes and adipocytes have not been examined. Read More

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May 2018
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