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    Eucalyptus globulus extract protects against UVB-induced photoaging by enhancing collagen synthesis via regulation of TGF-β/Smad signals and attenuation of AP-1.
    Arch Biochem Biophys 2017 Nov 14. Epub 2017 Nov 14.
    College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea. Electronic address:
    UV irradiation triggers the overproduction of matrix metalloproteinases and collagen degradation, which in turn causes increased pigmentation, dryness, and deep wrinkling of the skin. These chronic symptoms are collectively referred to as photoaging. Eucalyptus globulus is an evergreen tree that is widely used in cosmetics because of its antimicrobial activity. Read More

    One single salt bridge explains the different cytolytic activities shown by actinoporins sticholysin I and II from the venom of Stichodactyla helianthus.
    Arch Biochem Biophys 2017 Nov 11;636:79-89. Epub 2017 Nov 11.
    Departamento de Bioquímica y Biología Molecular I, Facultades de Química y Biología, Universidad Complutense, 28040 Madrid, Spain. Electronic address:
    Sticholysins I and II (StnI and StnII), α-pore forming toxins from the sea anemone Stichodactyla helianthus, are water-soluble toxic proteins which upon interaction with lipid membranes of specific composition bind to the bilayer, extend and insert their N-terminal α-helix, and become oligomeric integral membrane structures. The result is a pore that leads to cell death by osmotic shock. StnI and StnII show 93% of sequence identity, but also different membrane pore-forming activities. Read More

    Kinetics and thermodynamics of the thermal inactivation and chaperone assisted folding of zebrafish dihydrofolate reductase.
    Arch Biochem Biophys 2017 Nov 11. Epub 2017 Nov 11.
    Amity Institute of Biotechnology, Amity University, Sector 125, Noida, Uttar Pradesh 201313, India. Electronic address:
    The maintenance of thermal stability is a major issue in protein engineering as many proteins tend to form inactive aggregates at higher temperatures. Zebrafish DHFR, an essential protein for the survival of cells, shows irreversible thermal unfolding transition. The protein exhibits complete unfolding and loss of activity at 50 °C as monitored by UV-Visible, fluorescence and far UV-CD spectroscopy. Read More

    SNHG16/miR-216-5p/ZEB1 signal pathway contributes to the tumorigenesis of cervical cancer cells.
    Arch Biochem Biophys 2017 Nov 7;637:1-8. Epub 2017 Nov 7.
    Department of Obstetrics and Gynecology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, No.1665, KongJiang Road, YangPu District, Shanghai 200092, China. Electronic address:
    Long non-coding RNAs (lncRNAs) have been confirmed as crucial regulators in tumorgenesis. Small nucleolar RNA host gene 16 (SNHG16) has been recently uncovered to be a potential oncogene in several types of cancers. However, its expression level and potential role in cervical cancer remain uncertain. Read More

    JNK signaling pathway regulates sorbitol-induced Tau proteolysis and apoptosis in SH-SY5Y cells by targeting caspase-3.
    Arch Biochem Biophys 2017 Nov 7;636:42-49. Epub 2017 Nov 7.
    Department of Biochemistry, Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain. Electronic address:
    Growing evidence suggests that Diabetes Mellitus increases the risk of developing Alzheimer's disease. It is well known that hyperglycemia, a key feature of Diabetes Mellitus, may induce plasma osmolarity disturbances. Both hyperglycemia and hyperosmolarity promote the altered post-translational regulation of microtubule-associated protein Tau. Read More

    Physiological serum copper concentrations found in malignancies cause unfolding induced aggregation of human serum albumin in vitro.
    Arch Biochem Biophys 2017 Nov 6;636:71-78. Epub 2017 Nov 6.
    Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202 002, India. Electronic address:
    Malignancies are characterized by several drastic metabolic changes, one of which is a progressive rise in the levels of serum copper. This rise in serum copper is documented across all malignancies and across malignancies in several species. This study aims to explore in vitro the effect of increased copper levels on the structure of the blood protein human serum albumin. Read More

    Naringin prevents the inhibition of intestinal Ca(2+) absorption induced by a fructose rich diet.
    Arch Biochem Biophys 2017 Nov 7;636:1-10. Epub 2017 Nov 7.
    Laboratorio "Dr. Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Córdoba, Argentina. Electronic address:
    This study tries to elucidate the mechanisms by which fructose rich diets (FRD) inhibit the rat intestinal Ca(2+) absorption, and determine if any or all underlying alterations are prevented by naringin (NAR). Male rats were divided into: 1) controls, 2) treated with FRD, 3) treated with FRD and NAR. The intestinal Ca(2+) absorption and proteins of the transcellular and paracellular Ca(2+) pathways were measured. Read More

    Kinetic and structural characterization of a cis-3-Chloroacrylic acid dehalogenase homologue in Pseudomonas sp. UW4: A potential step between subgroups in the tautomerase superfamily.
    Arch Biochem Biophys 2017 Oct 27;636:50-56. Epub 2017 Oct 27.
    Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, Austin, TX, 78712, USA. Electronic address:
    A Pseudomonas sp. UW4 protein (UniProt K9NIA5) of unknown function was identified as similar to 4-oxalocrotonate tautomerase (4-OT)-like and cis-3-chloroacrylic acid dehalogenase (cis-CaaD)-like subgroups of the tautomerase superfamily (TSF). This protein lacks only Tyr-103 of the amino acids critical for cis-CaaD activity (Pro-1, His-28, Arg-70, Arg-73, Tyr-103, Glu-114). Read More

    Oxygen binding isotope effects of triazole-based HIV-1 reverse transcriptase inhibitors indicate the actual binding site.
    Arch Biochem Biophys 2017 Dec 27;635:87-95. Epub 2017 Oct 27.
    Institute of Applied Radiation Chemistry, Lodz University of Technology, 90-924 Lodz, Poland. Electronic address:
    Binding isotope effects (BIEs) associated with binding of four triazole-based ligands to HIV-1 reverse transcriptase have been calculated at the QM/MM MD level of theory. Two main binding sites: allosteric cavity and RNase H active site, as well as three other sites reported in the literature (the Knuckles, the NNRTI Adjacent, and Incoming Nucleotide Binding) have been considered. The interactions between inhibitors and these protein sites have been quantified by binding free energies obtained from free energy perturbation (FEP) calculations, supported by interaction energy analysis. Read More

    Ru/Fe bimetallic complexes: Synthesis, characterization, cytotoxicity and study of their interactions with DNA/HSA and human topoisomerase IB.
    Arch Biochem Biophys 2017 Oct 28;636:28-41. Epub 2017 Oct 28.
    Department of Biology, University of Rome Tor Vergata, 00133, Rome, Italy. Electronic address:
    Three ruthenium/iron-based compounds, 1: [Ru(MIm)(bipy)(dppf)]PF6 (MIm = 2-mercapto-1-methylimidazole anion), 2: [RuCl(Im)(bipy)(dppf)]PF6 (Im = imidazole), and 3: [Ru(tzdt)(bipy)(dppf)]PF6 (tzdt = 1,3-thiazolidine-2-thione anion) (dppf = 1,1'-bis(diphenylphosphine)ferrocene and bipy = 2,2'-bipyridine), were synthesized, and characterized by elemental analyses, conductivity, UV/Vis, IR, (1)H, (13)C and (31)P{1H} NMR spectroscopies, and by electrochemical technique. The complex 3 was also characterized by single-crystal X-ray. The three ruthenium(II) complexes show cytotoxicity against DU-145 (prostate carcinoma cells) and A549 (lung carcinoma cells) tumor cells. Read More

    Prothymosin α interacts with SET, ANP32A and ANP32B and other cytoplasmic and mitochondrial proteins in proliferating cells.
    Arch Biochem Biophys 2017 Dec 1;635:74-86. Epub 2017 Nov 1.
    Departamento de Bioquímica y Biología Molecular, CIBUS, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address:
    Prothymosin α (ProTα) is an acidic protein with a nuclear role related to the chromatin activity through its interaction with histones in mammalian cells. ProTα acts as an anti-apoptotic factor involved in the control of the apoptosome activity in the cytoplasm, however the mechanisms underlying this function are still known. ProTα shares similar biological functions with acidic nuclear-cytoplasmic shuttling proteins included in SET and ANP32 family members. Read More

    Sexual dimorphism in oxidant-induced adaptive homeostasis in multiple wild-type D. melanogaster strains.
    Arch Biochem Biophys 2017 Oct 31;636:57-70. Epub 2017 Oct 31.
    Leonard Davis School of Gerontology of the Ethel Percy Andrus Gerontology Center, The University of Southern California, Los Angeles, CA 00089-0191, USA,; Molecular and Computational Biology Program of the Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, The University of Southern California, Los Angeles, CA 90089-0191, USA. Electronic address:
    Sexual dimorphism includes the physical and reproductive differences between the sexes, including differences that are conserved across species, ranging from the common fruit fly, Drosophila melanogaster, to humans. Sex-dependent variations in adaptive homeostasis, and adaptive stress responses may offer insight into the underlying mechanisms for male and female survival differences and into differences in chronic disease incidence and severity in humans. Earlier work showed sex-specific differences in adaptive responses to oxidative stressors in hybrid laboratory strains of D. Read More

    Acetyl-CoA carboxylase in Escherichia coli exhibits a pronounced hysteresis when inhibited by palmitoyl-acyl carrier protein.
    Arch Biochem Biophys 2017 Oct 31. Epub 2017 Oct 31.
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, United States. Electronic address:
    Acetyl-CoA carboxylase (ACC) in bacteria is composed of three components: biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase. ACC catalyzes the first committed step in fatty acid synthesis: the carboxylation of acetyl-CoA to form malonyl-CoA via a two-step reaction. In the first half-reaction, biotin carboxylase catalyzes the ATP-dependent carboxylation of the vitamin biotin covalently linked to biotin carboxyl carrier protein. Read More

    Thermodynamics of cooperative binding of FAD to human NQO1: Implications to understanding cofactor-dependent function and stability of the flavoproteome.
    Arch Biochem Biophys 2017 Oct 31;636:17-27. Epub 2017 Oct 31.
    Department of Physical Chemistry, University of Granada, Granada, Spain. Electronic address:
    The stability of human flavoproteins strongly depends on flavin levels, although the structural and energetic basis of this relationship is poorly understood. Here, we report an in-depth analysis on the thermodynamics of FAD binding to one of the most representative examples of such relationship, NAD(P)H:quinone oxidoreductase 1 (NQO1). NQO1 is a dimeric enzyme that tightly binds FAD, which triggers large structural changes upon binding. Read More

    Isomer distribution of hydroxyoctadecadienoates (HODE) and hydroxyeicosatetraenoates (HETE) produced in the plasma oxidation mediated by peroxyl radical, peroxynitrite, hypochlorite, 15-lipoxygenase, and singlet oxygen.
    Arch Biochem Biophys 2017 Dec 31;635:96-101. Epub 2017 Oct 31.
    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan; Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, Japan. Electronic address:
    Free and ester forms of unsaturated fatty acids and cholesterol are oxidized in vivo by multiple oxidants to give diverse products. Some lipid oxidation is mediated by enzymes to selectively give specific products, while others proceed randomly to produce mixtures of many kinds of regioisomers and stereoisomers. The efficacy of antioxidants against lipid oxidation depends on the nature of the oxidants and therefore the identification of oxidant is important for understanding the roles and effects of lipid oxidation and antioxidants in vivo. Read More

    Insights on the conformational dynamics of human frataxin through modifications of loop-1.
    Arch Biochem Biophys 2017 Oct 30. Epub 2017 Oct 30.
    Instituto de Química y Físico-Química Biológicas, University of Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina. Electronic address:
    Human frataxin (FXN) is a highly conserved mitochondrial protein involved in iron homeostasis and activation of the iron-sulfur cluster assembly. FXN deficiency causes the neurodegenerative disease Friedreich's Ataxia. Here, we investigated the effect of alterations in loop-1, a stretch presumably essential for FXN function, on the conformational stability and dynamics of the native state. Read More

    CagL from Helicobacter pylori has ADP-ribosylation activity and exerts partial protective efficacy in mice.
    Arch Biochem Biophys 2017 Dec 31;635:102-109. Epub 2017 Oct 31.
    School of Biosciences and Veterinary Medicine, University of Camerino, via Gentile III da Varano, 62032 Camerino, Italy. Electronic address:
    Mono ADP-ribosyltransferases are a class of functionally conserved enzymes present in prokaryotic and eukaryotic organisms. In prokaryotes, mono ADP-ribose transfer enzymes often represent a family of exotoxins that display activity in a variety of bacteria responsible for causing disease in plants and animals. A bioinformatic approach has allowed us to identify that CagL gene from some Helicobacter pylori strains shares a sequence pattern with ADP-ribosylating toxins of the CT-group. Read More

    Effect of quercetin on cell protection via erythropoietin and cell injury of HepG2 cells.
    Arch Biochem Biophys 2017 Nov 8;636:11-16. Epub 2017 Nov 8.
    Laboratory of Bioenvironmental Sciences, Course of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
    Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, and EPO is known to have a cytoprotective effect. Read More

    Insights into the role of methionine synthase in the universal (13)C depletion in O- and N-methyl groups of natural products.
    Arch Biochem Biophys 2017 Dec 23;635:60-65. Epub 2017 Oct 23.
    Elucidation of Biosynthesis by Isotopic Spectrometry Group, Interdisciplinary Chemistry: Synthesis, Analysis, Modeling, Centre National de la Recherche Scientifique-University of Nantes, Unité Mixte de Recherche 6230, F-44322 Nantes, France. Electronic address:
    Many O-methyl and N-methyl groups in natural products are depleted in (13)C relative to the rest of the molecule. These methyl groups are derived from the C-1 tetrahydrofolate pool via l-methionine, the principle donor of methyl units. Depletion could occur at a number of steps in the pathway. Read More

    Changes on serum and hepatic lipidome after a chronic cadmium exposure in Wistar rats.
    Arch Biochem Biophys 2017 Dec 21;635:52-59. Epub 2017 Oct 21.
    Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Mexico. Electronic address:

    Regulation of glutamate dehydrogenase (GDH) in response to whole body freezing in wood frog liver linked to differential acetylation and ADP-ribosylation.
    Arch Biochem Biophys 2017 Oct 19;636:90-99. Epub 2017 Oct 19.
    Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada. Electronic address:
    Glutamate dehydrogenase (GDH) represents a critical enzyme catalyzing the reaction spanning amino acid catabolism, the Krebs cycle, and urea production in the wood frog. GDH breaks down glutamate and NAD(+) to generate α-ketoglutaric acid (α-KG), NADH and ammonium that can be metabolized to form urea. Purification of GDH from control and frozen male wood frog livers was performed using a two-step column chromatography procedure with a cation exchange column and a GTP-agarose affinity column. Read More

    Increase of Bacillus badius Phenylalanine dehydrogenase specificity towards phenylalanine substrate by site-directed mutagenesis.
    Arch Biochem Biophys 2017 Dec 16;635:44-51. Epub 2017 Oct 16.
    Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:
    Phenylalanine dehydrogenase (PheDH) is a key enzyme in medical diagnostic for determining the amount of phenylalanine to detect phenylketonuria (PKU) disease. However, determination of phenylalanine can be usually disturbed in presence of tyrosine in blood samples. Position N145 of B. Read More

    Sex-dependent impact of Scp-2/Scp-x gene ablation on hepatic phytol metabolism.
    Arch Biochem Biophys 2017 Dec 16;635:17-26. Epub 2017 Oct 16.
    Department of Physiology and Pharmacology, Texas A&M University, TVMC, College Station, TX 77843-4466, United States. Electronic address:
    While prior studies focusing on male mice suggest a role for sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x; DKO) on hepatic phytol metabolism, its role in females is unresolved. This issue was addressed using female and male wild-type (WT) and DKO mice fed a phytoestrogen-free diet without or with 0.5% phytol. Read More

    Procyanidins from Cinnamomi Cortex promote proteasome-independent degradation of nuclear Nrf2 through phosphorylation of insulin-like growth factor-1 receptor in A549 cells.
    Arch Biochem Biophys 2017 Dec 16;635:66-73. Epub 2017 Oct 16.
    Department of Drug Metabolism and Molecular Toxicology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-0392, Japan. Electronic address:
    Many lines of evidence demonstrate that transcription factor nuclear factor-E2-related factor 2 (Nrf2) plays essential roles in cancer cell proliferation and resistance to chemotherapy, thereby indicating that suppression of abnormal Nrf2 activation is needed for a new therapeutic approach. Our previous studies reported that procyanidins prepared from Cinnamomi Cortex extract (CCE) have an ability to suppress cytoprotective enzymes and cell proliferation in human cancer cells with activated Nrf2. In the present study, we investigated the mechanism of CCE procyanidin-mediated antagonization of Nrf2. Read More

    Genotoxic effect and antigen binding characteristics of SLE auto-antibodies to peroxynitrite-modified human DNA.
    Arch Biochem Biophys 2017 Dec 16;635:8-16. Epub 2017 Oct 16.
    Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
    Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease characterized by auto-antibodies against native deoxyribonucleic acid after modification and is one of the reasons for the development of SLE. Here, we have evaluated the structural perturbations in human placental DNA by peroxynitrite using spectroscopy, thermal denaturation and high-performance liquid chromatography (HPLC). Peroxynitrite is a powerful potent bi-functional oxidative/nitrative agent that is produced both endogenously and exogenously. Read More

    Mild palmitate treatment increases mitochondrial mass but does not affect EA.hy926 endothelial cells viability.
    Arch Biochem Biophys 2017 Nov 7;634:88-95. Epub 2017 Nov 7.
    Laboratory of Cellular Metabolism, Department of Biochemistry, Nencki Institute of Experimental Biology of the Polish Academy of Sciences, 3 Pasteura Str., 02-093 Warsaw, Poland.
    A dyslipidaemia-related increase of the concentration of long-chain fatty acids in the plasma is an important pathological factor substantially increasing risk of serious consequences in vascular endothelium. Inflammatory response, atherosclerosis and insulin resistance seem the most severe. Palmitate at excessive concentrations has been shown to have a harmful effect on endothelial cells impairing NO generation, stimulating reactive oxygen species (ROS) formation and affecting their viability. Read More

    GADD45 family proteins suppress JNK signaling by targeting MKK7.
    Arch Biochem Biophys 2017 Dec 14;635:1-7. Epub 2017 Oct 14.
    Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan. Electronic address:
    Growth arrest and DNA damage-inducible 45 (GADD45) family genes encode related proteins, including GADD45α, GADD45β, and GADD45γ. In HeLa cells, expression of GADD45 members is differentially regulated under a variety of environmental conditions, but thermal and genotoxic stresses induce the expression of all genes. The heat shock response of GADD45β is mediated by the heat shock transcription factor 1 (HSF1), and GADD45β is necessary for heat stress survival. Read More

    Dielectric properties of plasma membrane: A signature for dyslipidemia in diabetes mellitus.
    Arch Biochem Biophys 2017 Dec 10;635:27-36. Epub 2017 Oct 10.
    Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India. Electronic address:
    Dielectric properties of a living biological membrane play crucial role indicating the status of the cell in pathogenic or healthy condition. A distinct variation in membrane capacitance and impedance was observed for peripheral blood mononuclear cell (PBMC) suspensions for diabetic and diabetic-dyslipidemic subjects compared to healthy control. Low frequency region were explicitly considered in electrical analysis to address complex membrane dielectric factors that alter the system capacitance of a PBMC suspension. Read More

    Substitutions of S101 decrease proton and hydride transfers in the oxidation of betaine aldehyde by choline oxidase.
    Arch Biochem Biophys 2017 Nov 10;634:76-82. Epub 2017 Oct 10.
    Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30302-3965, United States.
    Choline oxidase oxidizes choline to glycine betaine, with two flavin-mediated reactions to convert the alcohol substrate to the carbon acid product. Proton abstraction from choline or hydrated betaine aldehyde in the wild-type enzyme occurs in the mixing time of the stopped-flow spectrophotometer, thereby precluding a mechanistic investigation. Mutagenesis of S101 rendered the proton transfer reaction amenable to study. Read More

    STM2360 encodes a d-ornithine/d-lysine decarboxylase in Salmonella enterica serovar typhimurium.
    Arch Biochem Biophys 2017 Nov 9;634:83-87. Epub 2017 Oct 9.
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
    STM2360 is a gene located in a small operon of undetermined function in Salmonella enterica serovar Typhimurium LT2. The amino acid sequence of STM2360 shows significant similarity (∼30% identity) to diaminopimelate decarboxylase (DapDC), a Fold III pyridoxal-5'-phosphate (PLP) dependent enzyme involved in l-lysine biosynthesis. We have found that the protein coded by STM2360 has a previously undocumented catalytic activity, d-ornithine/d-lysine decarboxylase (DOKDC). Read More

    Characterization of the nuclear import pathway for BLM protein.
    Arch Biochem Biophys 2017 Nov 7;634:57-68. Epub 2017 Oct 7.
    College of Animal Science, Guizhou University, Guiyang 550025, China.
    Numerous studies have shown that nuclear localization of BLM protein, a member of the RecQ helicases, mediated by nuclear localization signal (NLS) is critical for DNA recombination, replication and transcription, but the mechanism by which BLM protein is imported into the nucleus remains unknown. In this study, the nuclear import pathway for BLM was investigated. We found that nuclear import of BLM was inhibited by two dominant-negative mutants of importin β1 and NTF2/E42K, which lacks the ability to bind Ran and RanGDP, respectively, but was not inhibited by the Ran/Q69L, which is deficient in GTP hydrolysis. Read More

    Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing.
    Arch Biochem Biophys 2017 Dec 6;635:37-43. Epub 2017 Oct 6.
    Institute of Automation and Electrometry, Russian Academy of Sciences, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia.
    Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. Read More

    Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage.
    Arch Biochem Biophys 2017 Nov 3;634:69-75. Epub 2017 Oct 3.
    Department of Biochemistry and Molecular Biology, Yeungnam University College of Medicine, Daegu 42415, Republic of Korea. Electronic address:
    Acetaminophen (APAP) overdose induces acute liver damage and failure via reactive oxygen species production and glutathione (GSH) depletion. Methionine sulfoxide reductase B1 (MsrB1) is an antioxidant selenoenzyme that specifically catalyzes the reduction of methionine R-sulfoxide residues. In this study, we used MsrB1 gene-knockout mice and primary hepatocytes to investigate the effect of MsrB1 on APAP-induced hepatotoxicity. Read More

    Evolutionary conservation of EF-hand ΙΙ loop in aequorin: Priority of intensity to decay rate in bioluminescence emission.
    Arch Biochem Biophys 2017 Nov 29;634:29-37. Epub 2017 Sep 29.
    Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:
    As a Ca(2+)-regulated photoprotein, aequorin (Aeq) contains four EF-hand motifs, the second one lacks the standard sequence for Ca(2+) coordination and doesn't bind to Ca(2+). Here, we replaced this loop with a functional loop. According to structural studies, although the global stability of modified aequorin (4EFAeq) is higher than that of Aeq; increasing the local flexibility accompanied by internal structural rearrangements in 4EFAeq result in its penetrability to urea and acrylamide. Read More

    The inhibitory effects of biomimetically designed peptides on α-synuclein aggregation.
    Arch Biochem Biophys 2017 Nov 28;634:96-106. Epub 2017 Sep 28.
    Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
    Parkinson's disease is characterized by accumulation of inclusion bodies in dopaminergic neurons, where insoluble and fibrillar α-synuclein makes up the major component of these inclusion bodies. So far, several strategies have been applied in order to suppress α-synuclein aggregation and toxicity in Parkinson's disease. In the present study, a new database has been established by segmentation of all the proteins deposited in protein Data Bank. Read More

    Functional significance of C-terminal mobile domain of cardiac troponin I.
    Arch Biochem Biophys 2017 Nov 27;634:38-46. Epub 2017 Sep 27.
    The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA; The Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA 99164, USA. Electronic address:
    Ca(2+)-regulation of cardiac contractility is mediated through the troponin complex, which comprises three subunits: cTnC, cTnI, and cTnT. As intracellular [Ca(2+)] increases, cTnI reduces its binding interactions with actin to primarily interact with cTnC, thereby enabling contraction. A portion of this regulatory switching involves the mobile domain of cTnI (cTnI-MD), the role of which in muscle contractility is still elusive. Read More

    A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid's proteome.
    Arch Biochem Biophys 2017 Nov 23;634:1-10. Epub 2017 Sep 23.
    iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal; Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Universidade do Porto, Porto, Portugal.
    Owing to their close proximity, pericardial fluid (PF)'s proteome may mirror the pathophysiological status of the heart. Despite this diagnosis potential, the knowledge of PF's proteome is scarce. Large amounts of albumin hamper the characterization of the least abundant proteins in PF. Read More

    All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids.
    Arch Biochem Biophys 2017 Nov 23;634:21-28. Epub 2017 Sep 23.
    Department of Ophthalmology and Visual Sciences, University of Utah, 65 N Mario Capecchi Dr, Salt Lake City, UT, United States; Department of Neurobiology and Anatomy, University of Utah, 20 S 2030 E, Salt Lake City, UT, United States. Electronic address:
    Carotenoids are plant pigment molecules that are potent antioxidants. Carotenoids cannot be synthesized de novo; therefore, their dietary intake and transport to various tissues are essential to harness their health benefits. Two of the three scavenger receptor class B (SRB) proteins, SR-B1 and CD36, have been implicated as carotenoid transporters in lower species and in various tissues of higher animals. Read More

    DJ-1 activates autophagy in the repression of cardiac hypertrophy.
    Arch Biochem Biophys 2017 Nov 21;633:124-132. Epub 2017 Sep 21.
    Department of Cardiology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; Key Laboratory on Assisted Circulation, Ministry of Health, Guangzhou, China. Electronic address:
    Cardiac hypertrophy is the risk factor of heart failure when the heart is confronted with pressure overload or neurohumoral stimuli. Autophagy, a conserved degradative pathway, is one of the important mechanisms involved in the regulation of cardiac hypertrophy. DJ-1 is a traditional anti-oxidative protein and emerging evidence suggested that DJ-1 might modulate autophagy. Read More

    CFTR modulates RPS27 gene expression using chloride anion as signaling effector.
    Arch Biochem Biophys 2017 Nov 21;633:103-109. Epub 2017 Sep 21.
    Institute for Biomedical Research (BIOMED, UCA-CONICET), Laboratory of Cellular and Molecular Biology, School of Medical Sciences, Pontifical Catholic University of Argentina (UCA) and The National Scientific and Technical Research Council of Argentina (CONICET), Buenos Aires, C1107AFF, Argentina. Electronic address:
    In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Cl(-) concentrations ([Cl(-)]i), we observed several Cl(-)-dependent genes and further characterized one of them as RPS27. Read More

    Reaction rates of glutathione and ascorbate with alkyl radicals are too slow for protection against protein peroxidation in vivo.
    Arch Biochem Biophys 2017 Nov 20;633:118-123. Epub 2017 Sep 20.
    Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
    Reaction kinetics of amino acid and peptide alkyl radicals with GSH and ascorbate, the two most abundant endogenous antioxidants, were investigated by pulse radiolysis. Rate constants in the order of 10(6) M(-1)s(-1) were found. Alkyl radicals react at almost diffusion controlled rates and irreversibly with oxygen to form peroxyl radicals, and competition with this reaction is the benchmark for efficient repair in vivo. Read More

    Retinol saturase modulates lipid metabolism and the production of reactive oxygen species.
    Arch Biochem Biophys 2017 Nov 18;633:93-102. Epub 2017 Sep 18.
    Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS 66045, USA. Electronic address:
    Retinol saturase (RetSat) catalyzes the saturation of double bonds of all-trans-retinol leading to the production of dihydroretinoid metabolites. Beside its role in retinoid metabolism, there is evidence that RetSat modulates the cellular response to oxidative stress and plays critical roles in adipogenesis and the accumulation of lipids. Here, we explore the relationship between RetSat, lipid metabolism and oxidative stress using in vitro and in vivo models with altered expression of RetSat. Read More

    ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activity.
    Arch Biochem Biophys 2017 Nov 14;633:85-92. Epub 2017 Sep 14.
    Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. Electronic address:
    The genome of Methanosarcinales organisms presents both ADP-dependent glucokinase and phosphofructokinase genes. However, Methanococcoides burtonii has a truncate glucokinase gene with a large deletion at the C-terminal, where the catalytic GXGD motif is located. Characterization of its phosphofructokinase annotated protein shows that is a bifunctional enzyme able to supply the absence of the glucokinase activity. Read More

    ATP alters protein folding and function of Escherichia coli uridine phosphorylase.
    Arch Biochem Biophys 2017 Nov 13;634:11-20. Epub 2017 Sep 13.
    Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan, ROC. Electronic address:
    Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Read More

    The soluble domains of Gpi8 and Gaa1, two subunits of glycosylphosphatidylinositol transamidase (GPI-T), assemble into a complex.
    Arch Biochem Biophys 2017 Nov 8;633:58-67. Epub 2017 Sep 8.
    Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA; Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA. Electronic address:
    Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active site subunit of GPI-T, is part of a hetero-pentameric complex containing Gpi16, Gaa1, Gpi17, and Gab1. Gpi8, Gaa1, and Gpi16 co-purify as a heterotrimer from Saccharomyces cerevisiae, suggesting that they form the core of the GPI-T. Read More

    Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells.
    Arch Biochem Biophys 2017 Nov 9;633:68-77. Epub 2017 Sep 9.
    Center of Medical Physics and Technology, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Anhui Province, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou, Jiangsu, China. Electronic address:
    Non-thermal plasma (NTP) has been proposed as a novel therapeutic method for anticancer treatment. Although increasing evidence suggests that NTP selectively induces apoptosis in some types of tumor cells, the molecular mechanisms underlying this phenomenon remain unclear. In this study, we further investigated possible molecular mechanisms for NTP-induced apoptosis of HeLa cells. Read More

    The catalytic activity of a recombinant single chain variable fragment nucleic acid-hydrolysing antibody varies with fusion tag and expression host.
    Arch Biochem Biophys 2017 Nov 6;633:110-117. Epub 2017 Sep 6.
    Department of Biomedical Sciences, Graduate School, Ajou University, 206 World Cup-ro, Yeongtong-gu, Suwon 16499, South Korea; Department of Microbiology, Ajou University School of Medicine, 206 World Cup-ro, Yeongtong-gu, Suwon 16499, South Korea. Electronic address:
    The antigen-binding properties of single chain Fv antibodies (scFvs) can vary depending on the position and type of fusion tag used, as well as the host cells used for expression. The issue is even more complicated with a catalytic scFv antibody that binds and hydrolyses a specific antigen. Herein, we investigated the antigen-binding and -hydrolysing activities of the catalytic anti-nucleic acid antibody 3D8 scFv expressed in Escherichia coli or HEK293f cells with or without additional amino acid residues at the N- and C-termini. Read More

    Interplay of mitochondria apoptosis regulatory factors and microRNAs in valvular heart disease.
    Arch Biochem Biophys 2017 Nov 6;633:50-57. Epub 2017 Sep 6.
    Signal Transduction Lab, Department of Bio-Chemistry, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan. Electronic address:
    Valvular heart disease (VHD) is an active process involving a wide range of pathological changes. The major complications of VHD are stenosis and regurgitation, which are macroscopic phenomena, induced in part through cellular changes. Altered expression of mitochondria associated genes causes membrane potential depolarization, leading to the increased levels of apoptosis observed in cardiac dysfunction. Read More

    Disulfiram is a slow-binding partial noncompetitive inhibitor of 20S proteasome activity.
    Arch Biochem Biophys 2017 Nov 5;633:23-28. Epub 2017 Sep 5.
    College of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, Manitoba R3E 0T5, Canada.
    The alcohol abuse drug disulfiram has also been shown to exhibit potent cell growth inhibitory and anticancer activity. While a number of cellular and animal studies have suggested that disulfiram exhibits its anticancer activity through interaction with the proteasome, direct evidence for inhibition of proteasome activity is lacking. In this study we show that disulfiram potently inhibits the chymotrypsin-like activity of purified human 20S proteasome at low micromolar pharmacological concentrations. Read More

    Bisphenol A and estrogen induce proliferation of human thyroid tumor cells via an estrogen-receptor-dependent pathway.
    Arch Biochem Biophys 2017 Nov 4;633:29-39. Epub 2017 Sep 4.
    Department of Endocrinology, Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address:
    Objective: To determine the relationship between papillary thyroid carcinoma and environmental exposure to bisphenol A (BPA) or 17-β estrogen (E2) by assessing the effects of these compounds on estrogen receptor expression and AKT/mTOR signaling.

    Methods: The effects of low levels of BPA (1mM-10nM) and 17β-estradiol (E2, 0.1mM-1nM) on ER expression and cellular proliferation were determined in human thyroid papillary cancer BHP10-3 cells. Read More

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