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    29060 results match your criteria Archives of Biochemistry and Biophysics[Journal]

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    Posttranslational modification of heme in peroxidases - Impact on structure and catalysis.
    Arch Biochem Biophys 2018 Feb 17. Epub 2018 Feb 17.
    Department of Chemistry, Division of Biochemistry, Protein Biochemistry, VIBT - Vienna Institute of BioTechnology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria. Electronic address:
    Four heme peroxidase superfamilies arose independently in evolution. Only in the peroxidase-cyclooxygenase superfamily the prosthetic group is posttranslationally modified (PTM). As a consequence these peroxidases can form one, two or three covalent bonds between heme substituents and the protein. Read More

    Mechanistic studies of formate oxidase from Aspergillus oryzae: A novel member of the glucose-Methanol-choline oxidoreductase enzyme superfamily that oxidizes carbon acids.
    Arch Biochem Biophys 2018 Feb 16. Epub 2018 Feb 16.
    Department of Chemistry, Georgia State University, Atlanta, GA, 30302-3965, United States; Department of Biology, Georgia State University, Atlanta, GA, 30302-4010, United States; Center for Biotechnology and Drug Design, Georgia State University, Atlanta, GA, 30302-3965, United States; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, 30302-3965, United States. Electronic address:
    Formate oxidase (FOX) from Aspergillus oryzae is the only GMC member that oxidizes a carbon acid rather than alcohols; thus, its catalytic mechanism may be different from that of other GMC members. We have used pH, solvent viscosity, and deuterium kinetic isotope effects, to investigate the catalytic mechanism of FOX. The enzyme followed a Bi-Bi sequential steady-state kinetic mechanism. Read More

    14-3-3ζ promotes esophageal squamous cell carcinoma invasion by repressing S1PR2 protein expression through NF- κB signaling.
    Arch Biochem Biophys 2018 Feb 16. Epub 2018 Feb 16.
    Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
    14-3-3ζ is overexpressed in several cancers, including esophageal squamous cell carcinoma (ESCC), and plays an important role in tumorigenesis. However, the mechanisms underlying its tumorigenesis remain unclear. Here we report that 14-3-3ζ was upregulated in ESCC tumors, compared with adjacent normal tissues; 14-3-3ζ levels were positively correlated with ESCC lymph node metastasis and recurrence. Read More

    Inactivation of Escherichia coli and Staphylococcus aureus on contaminated perilla leaves by Dielectric Barrier Discharge (DBD) plasma treatment.
    Arch Biochem Biophys 2018 Feb 15. Epub 2018 Feb 15.
    Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea. Electronic address:
    This study focused on sterilization methods for the reduction of microorganisms on perilla leaves by cylinder type Dielectric Barrier Discharge (DBD) plasma with underwater bubbler treatment. S. aureus and E. Read More

    Effect of glycated insulin on the blood-brain barrier permeability: An in vitro study.
    Arch Biochem Biophys 2018 Feb 11. Epub 2018 Feb 11.
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
    Alteration in permeability of blood-brain barrier (BBB) is of immense importance in the pathogenesis of diabetes-related central nervous system disorders. Considering the presence of glycated insulin in plasma of type 2 diabetic patients, we hypothesized that glycated insulin could induce changes in paracellular permeability in BBB. Therefore, the authors decided to study the effect of glycated insulin on paracellular permeability in a BBB model. Read More

    Suppressive effects of aryl-hydrocarbon receptor repressor on adipocyte differentiation in 3T3-L1 cells.
    Arch Biochem Biophys 2018 Feb 9;642:75-80. Epub 2018 Feb 9.
    Center for Health and the Environment, University of California, Davis, Davis, CA, 95616, USA; Department of Environmental Toxicology, University of California, Davis, Davis, CA, 95616, USA.
    The aryl-hydrocarbon receptor repressor (AhRR) negatively regulates aryl-hydrocarbon receptor (AhR) signaling via its inhibitory transactivation. AhR is well known to suppress adipocyte differentiation, but the function of AhRR during adipogenesis is unclear. The purpose of this study was to investigate the role of AhRR in adipocyte differentiation using 3T3-L1 cells. Read More

    The structurally similar TRFH domain of TRF1 and TRF2 dimers shows distinct behaviour towards TIN2.
    Arch Biochem Biophys 2018 Feb 8;642:52-62. Epub 2018 Feb 8.
    Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Narutowicza St 11/12, 80-233 Gdansk, Poland.
    The telomere repeat binding-factor 1 and 2 (TRF1 and TRF2) proteins of the shelterin complex bind to duplex telomeric DNA as homodimers, and the homodimerization is mediated by their TRFH (TRF-homology) domains. We performed molecular dynamic (MD) simulations of the dimer forms of TRF1and TRF2in the presence/absence of the TIN2(TIN2, TRF-interacting nuclear protein 2, TBM, TRF-binding motif) peptide. The MD results suggest that TIN2is necessary to ensure the stability of TRF1homodimer but not the TRF2homodimer. Read More

    Antioxidant potentials of nanoceria synthesized by solution plasma process and its biocompatibility study.
    Arch Biochem Biophys 2018 Feb 7. Epub 2018 Feb 7.
    Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea; Centre for Surface Technology and Applications, Korea Aerospace University, Goyang, 10540, Republic of Korea. Electronic address:
    Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25min using a pulsed unipolar power supply into solutions containing Ce(NO)(1 and 2 mM). UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the peaks as the concentration of Ce(NO)increased. The peaks exhibited transition to red shift due to nanoceria formation. Read More

    Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems.
    Arch Biochem Biophys 2018 Feb 1;642:46-51. Epub 2018 Feb 1.
    Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:
    Apaf-1 is a cytosolic multi-domain protein in the apoptosis regulatory network. When cytochrome c releases from mitochondria; it binds to WD-40 repeats of Apaf-1 molecule and induces oligomerization of Apaf-1. Here in, a split luciferase assay was used to compare apoptosome formation in cell-free and cell-based systems. Read More

    A pivotal role for NF-κB in the macrophage inflammatory response to the myeloperoxidase oxidant hypothiocyanous acid.
    Arch Biochem Biophys 2018 Feb 2;642:23-30. Epub 2018 Feb 2.
    Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia; Department of Biomedical Sciences, University of Copenhagen, Panum Institute, Blegdamsvej 3, Copenhagen N DK-2200, Denmark. Electronic address:
    Atherosclerosis is characterised by the infiltration of macrophages at sites of inflammation within the vessel wall and the release of myeloperoxidase (MPO), which forms hypochlorous acid (HOCl) and hypothiocyanous acid (HOSCN). HOCl is a damaging oxidant implicated in the development of atherosclerosis. Preferential formation of HOSCN occurs under conditions where thiocyanate ions are elevated, as is the case in smokers. Read More

    Part-of-the-sites binding and reactivity in the homooligomeric enzymes - facts and artifacts.
    Arch Biochem Biophys 2018 Feb 8;642:31-45. Epub 2018 Feb 8.
    Division of Biophysics, Institute of Experimental Physics, Department of Physics, University of Warsaw, Pasteura 5, Warsaw, 02-093, Poland. Electronic address:
    For a number of enzymes composed of several subunits with the same amino acid sequence, it was documented, or suggested, that binding of a ligand, or catalysis, is carried out by a single subunit. This phenomenon may be the result of a pre-existent asymmetry of subunits or a limiting case of the negative cooperativity, and is sometimes called "half-of-the-sites binding (or reactivity)" for dimers and could be called "part-of-the-sites binding (or reactivity)" for higher oligomers. In this article, we discuss molecular mechanisms that may result in "part-of-the-sites binding (and reactivity)", offer possible explanations why it may have a beneficial role in enzyme function, and point to experimental problems in documenting this behaviour. Read More

    Biosynthesis of human myeloperoxidase.
    Arch Biochem Biophys 2018 Feb 3;642:1-9. Epub 2018 Feb 3.
    Inflammation Program and Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine University of Iowa, Veterans Administration Medical Center, Iowa City, IA 52240, USA; Veterans Administration Medical Center, Iowa City, IA 52240, USA; 2501 Crosspark Road D168- MTF, Coralville, IA 52241, USA. Electronic address:
    Members of Chordata peroxidase subfamily [1] expressed in mammals, including myeloperoxidase (MPO), eosinophil peroxidase (EPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO), express conserved motifs around the heme prosthetic group essential for their activity, a calcium-binding site, and at least two covalent bonds linking the heme group to the protein backbone. Although most studies of the biosynthesis of these peroxidases have focused on MPO, many of the features described occur during biosynthesis of other members of the protein subfamily. Whereas MPO biosynthesis includes events typical for proteins generated in the secretory pathway, the importance and consequences of heme insertion are events uniquely associated with peroxidases. Read More

    Binding of alpha-synuclein to partially oxidized glyceraldehyde-3-phosphate dehydrogenase induces subsequent inactivation of the enzyme.
    Arch Biochem Biophys 2018 Feb 3;642:10-22. Epub 2018 Feb 3.
    Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119234, Russia. Electronic address:
    According to literature data, the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) co-localizes with alpha-synuclein in Lewy bodies in Parkinson's disease, which suggests the involvement of this protein in the development of synucleinopathies. The goal of the present work was to investigate the direct interaction between alpha-synuclein and GAPDH and to evaluate possible influence of this interaction on the catalytic properties of GAPDH. Molecular dynamic simulations predicted the binding of alpha-synuclein to the positively charged groove comprising NAD-binding pocket of GAPDH. Read More

    Promoter analysis and transcriptional regulation of human carbonic anhydrase VIII gene in a MERRF disease cell model.
    Arch Biochem Biophys 2018 Mar 31;641:50-61. Epub 2018 Jan 31.
    Department of Life Science, Tunghai University, Taichung, Taiwan, ROC; Life Science Research Center, Tunghai University, Taichung, Taiwan, ROC. Electronic address:
    Myoclonic epilepsy with ragged-red fibers (MERRF) is a maternally inherited mitochondrial neuromuscular disease. We previously reported a significant decrease of mRNA and protein levels of nuclear DNA-encoded carbonic anhydrase VIII (CA8) in MERRF cybrids harboring A8344G mutation in mitochondrial DNA (mtDNA). In this study, we established a reporter construct of luciferase gene-carrying hCA8 promoter containing several putative transcription factor-binding sites, including GC-box, AP-2 and TATA-binding element in the 5'flanking region of the hCA8 gene. Read More

    Structure and function of heme proteins regulated by diverse post-translational modifications.
    Arch Biochem Biophys 2018 Mar 2;641:1-30. Epub 2018 Feb 2.
    School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China; Laboratory of Protein Structure and Function, University of South China, Hengyang 421001, China. Electronic address:
    Heme proteins are crucial for biological systems by performing diverse functions. Nature has evolved diverse approaches to fine-tune the structure and function of heme proteins, of which post-translational modification (PTM) is a primary method. As reviewed herein, a multitude of PTMs have been discovered for heme proteins in the last several decades, including heme-protein cross-links with heme side chains (Cys-heme, Tyr-heme and Asp/Glu-heme, etc) or porphyrin ring (Lys-heme and Tyr-heme, etc), heme modifications (sulfheme and nitriheme, etc), amino acids cross-links between two or among multiple residues (Cys-Cys, Tyr-His, Tyr-Cys, Met-Tyr-Trp, etc), and amino acids modifications by oxidation, nitration, phosphorylation and glycation, etc. Read More

    A role for glutamine 183 in the folate oxidative half-reaction of methylenetetrahydrofolate reductase from Escherichia coli.
    Arch Biochem Biophys 2018 Feb 3;642:63-74. Epub 2018 Feb 3.
    Department of Chemistry, Grinnell College, Grinnell, IA 50112, USA. Electronic address:
    The flavoprotein methylenetetrahydrofolate reductase (MTHFR) from Escherichia coli catalyzes a ping-pong reaction with NADH and 5,10-methylenetetrahydrofolate (CH-Hfolate) to produce NADand 5-methyltetrahydrofolate (CH-Hfolate). This work focuses on the function of the invariant, active-site aminoacyl residue Gln183. X-ray structures of the enzyme complexes E(wild-type)•NADH and E(Glu28Gln)•CH-Hfolate indicate that Gln183 makes key hydrogen-bonding interactions with both NADH and folate in their respective half-reactions, suggesting roles in binding each substrate. Read More

    SAXS and homology modelling based structure characterization of pectin methylesterase a family 8 carbohydrate esterase from Clostridium thermocellum ATCC 27405.
    Arch Biochem Biophys 2018 Mar 31;641:39-49. Epub 2018 Jan 31.
    Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India. Electronic address:
    Pectin methylesterase (CtPME) from Clostridium thermocellum of family 8 carbohydrate esterase (CE8) belongs to pectin methylesterase super family (E.C.3. Read More

    The chlorinated lipidome originating from myeloperoxidase-derived HOCl targeting plasmalogens: Metabolism, clearance, and biological properties.
    Arch Biochem Biophys 2018 Mar 31;641:31-38. Epub 2018 Jan 31.
    Edward A. Doisy Department of Biochemistry and Molecular Biology and the Center for Cardiovascular Research, Saint Louis University School of Medicine, St. Louis, MO, United States. Electronic address:
    Myeloperoxidase produces the two-electron oxidant HOCl, which targets plasmalogen phospholipids liberating 2-chlorofatty aldehyde. 2-Chlorofatty aldehyde has four known fates: 1) oxidation to 2-chlorofatty acid; 2) reduction to 2-chlorofatty alcohol; 3) Schiff base adduct formation with proteins and amines; and 4) reactivity with glutathione through nucleophilic attack of the α-chlorinated carbon. 2-Chlorofatty acid does not undergo conventional fatty acid β-oxidation due to the presence of the α-chlorinated carbon; however, 2-chlorofatty acid does undergo sequential ω-oxidation and β-oxidation from the ω-end, ultimately resulting in 2-chloroadipic acid urinary excretion. Read More

    Long non-coding RNA CASC2 regulates Sprouty2 via functioning as a competing endogenous RNA for miR-183 to modulate the sensitivity of prostate cancer cells to docetaxel.
    Arch Biochem Biophys 2018 Jan 23. Epub 2018 Jan 23.
    Department of Urology Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address:
    Prostate cancer (PC) is the most common cancer in men; however, limited effect is obtained due to the therapy resistance. CASC2 acts as a tumor suppressor in human malignancies serving as a ceRNA for miRNAs; Sprouty2 (SPRY2), a key antagonist of RTK signalling, also serves as a tumor suppressor. Herein, CASC2 and SPRY2 expression was down-regulated in PC tissues and cell lines; the overexpression of CASC2 and SPRY2 could suppress PC cell proliferation, promote PC cell apoptosis, and enhance the sensitivity of PC cells to docetaxel. Read More

    Product specificity of fungal 8R- and 9S-dioxygenases of the peroxidase-cyclooxygenase superfamily with amino acid derivatized polyenoic fatty acids.
    Arch Biochem Biophys 2018 Feb 17;640:93-101. Epub 2018 Jan 17.
    Division of Biochemical Pharmacology, Department of Pharmaceutical Biosciences, Uppsala University, Box 591, SE-751 24 Uppsala, Sweden. Electronic address:
    Pathogenic fungi express fatty acid dioxygenases (DOX) fused to cytochromes P450 with diol or allene oxide synthase activities. The orientation of the fatty acids in the active sites of DOX was investigated with amino acid conjugates of 18:3n-3 and 18:2n-6. 9S-DOX-allene oxide synthase (AOS) oxidized the Gly, Ile, and Trp derivatives at C-9, which suggests that these conjugates enter the substrate recognition site with the omega end in analogy with fatty acids bound to cyclooxygenases and coral 8R-lipoxygenase (8R-LOX). Read More

    Polyphyllin G exhibits antimicrobial activity and exerts anticancer effects on human oral cancer OECM-1 cells by triggering G2/M cell cycle arrest by inactivating cdc25C-cdc2.
    Arch Biochem Biophys 2018 Jan 15. Epub 2018 Jan 15.
    Oral and Maxillofacial Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, 450000, PR China.
    Plant natural products have long been considered to be important sources of bioactive molecules. A large number of antimicrobial and anticancer agents have been isolated form plants. In the present study we evaluated the antimicrobial and anticancer activity of a plant derived secondery metabolite, Polyphyllin G. Read More

    The proteoglycan biglycan mediates inflammatory response by activating TLR-4 in human chondrocytes: Inhibition by specific siRNA and high polymerized Hyaluronan.
    Arch Biochem Biophys 2018 Feb 13;640:75-82. Epub 2018 Jan 13.
    Department of Clinical and Experimental Medicine, University of Messina, Policlinico Universitario, 98125 Messina, Italy. Electronic address:
    Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. Read More

    Structural and biochemical analyses reveal ubiquitin C-terminal hydrolase-L1 as a specific client of the peroxiredoxin II chaperone.
    Arch Biochem Biophys 2018 Feb 12;640:61-74. Epub 2018 Jan 12.
    School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, South Korea. Electronic address:
    Peroxiredoxins (Prxs) play dual roles as both thiol-peroxidases and molecular chaperones. Peroxidase activity enables various intracellular functions, however, the physiological roles of Prxs as chaperones are not well established. To study the chaperoning function of Prx, we previously sought to identify heat-induced Prx-binding proteins as the clients of a Prx chaperone. Read More

    Myeloperoxidase: Its role for host defense, inflammation, and neutrophil function.
    Arch Biochem Biophys 2018 Feb 11;640:47-52. Epub 2018 Jan 11.
    Graduate School of Nanobioscience, Yokohama City University, Seto 22-2, Kanazawa, Yokohama 236-0027, Japan. Electronic address:
    Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in microbial killing by neutrophils. Read More

    Baicalin inhibits pressure overload-induced cardiac fibrosis through regulating AMPK/TGF-β/Smads signaling pathway.
    Arch Biochem Biophys 2018 Feb 10;640:37-46. Epub 2018 Jan 10.
    Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511436, PR China. Electronic address:
    AMP-activated protein kinase (AMPK) is a central regulator of multiple metabolic pathways. It has been shown that activation of AMPK could inhibit fibroblast proliferation and extracellular matrix (ECM) accumulation, thereby suppressing cardiac fibrosis. Baicalin, the major component found in skullcap, possesses multiple protective effects on the cardiovascular system. Read More

    Coproheme decarboxylases - Phylogenetic prediction versus biochemical experiments.
    Arch Biochem Biophys 2018 Feb 10;640:27-36. Epub 2018 Jan 10.
    Department of Chemistry, Division of Biochemistry, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria. Electronic address:
    Coproheme decarboxylases (ChdCs) are enzymes responsible for the catalysis of the terminal step in the coproporphyrin-dependent heme biosynthesis pathway. Phylogenetic analyses confirm that the gene encoding for ChdCs is widespread throughout the bacterial world. It is found in monoderm bacteria (Firmicutes, Actinobacteria), diderm bacteria (e. Read More

    MiR-186-5p upregulation inhibits proliferation, metastasis and epithelial-to-mesenchymal transition of colorectal cancer cell by targeting ZEB1.
    Arch Biochem Biophys 2018 Feb 8;640:53-60. Epub 2018 Jan 8.
    Department of Colorectal Surgery, The First Affiliated Hospital of Wenzhou Medical University, 2 Fuxue Lane, Wenzhou, Zhejiang, 325000, China. Electronic address:
    MicroRNA-186-5p (miR-186-5p) is upregulated and exhibits as a crucial oncogene in various human tumors. However, the functions and underlying mechanisms of this microRNA on colorectal cancer remain largely unknown. Here, we report that miR-186-5p share a lower expression in colorectal cancer cell lines (HT116, H29, SW620 and LoVo) than in normal colonic epithelial cell line NCM460. Read More

    Functions and dysfunctions of Ca/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E.
    Arch Biochem Biophys 2018 Feb 6;640:83-92. Epub 2018 Jan 6.
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan.
    Intracellular signal transduction is built on the basis of the subtle balance between phosphorylation and dephosphorylation. Ca/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F/POPX2) and CaMKP-N (PPM1E/POPX1) are Ser/Thr phosphatases that belong to the PPM (protein phosphatase, Mg/Mn-dependent) family. The former was discovered in rat brain as a novel protein phosphatase regulating Ca/calmodulin-dependent protein kinases (CaMKs), whereas the latter was first identified in human cDNA databases using the rat CaMKP sequence. Read More

    Prolonged exposure to 1,25(OH)Dand high ionized calcium induces FGF-23 production in intestinal epithelium-like Caco-2 monolayer: A local negative feedback for preventing excessive calcium transport.
    Arch Biochem Biophys 2018 Feb 6;640:10-16. Epub 2018 Jan 6.
    Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand. Electronic address:
    Overdose of oral calcium supplement and excessive intestinal calcium absorption can contribute pathophysiological conditions, e.g., nephrolithiasis, vascular calcification, dementia, and cardiovascular accident. Read More

    Structure, kinetics, molecular and redox properties of a cytosolic and developmentally regulated fungal catalase-peroxidase.
    Arch Biochem Biophys 2018 Feb 2;640:17-26. Epub 2018 Jan 2.
    Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, UNAM, Mexico. Electronic address:
    CAT-2, a cytosolic catalase-peroxidase (CP) from Neurospora crassa, which is induced during asexual spore formation, was heterologously expressed and characterized. CAT-2 had the Met-Tyr-Trp (M-Y-W) adduct required for catalase activity. Its Kfor HOwas micromolar for peroxidase and millimolar for catalase activity. Read More

    Progress curve analysis of the kinetics of slow-binding anticancer drug inhibitors of the 20S proteasome.
    Arch Biochem Biophys 2018 02 2;639:52-58. Epub 2018 Jan 2.
    College of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, Manitoba R3E 0T5, Canada. Electronic address:
    Bortezomib, carfilzomib, ixazomib, oprozomib, and delanzomib are anticancer drugs that target the proteasomal system. Carfilzomib and oprozomib are epoxyketones that form an irreversible covalent bond with the 20S proteasome, whereas bortezomib, ixazomib, and delanzomib are boronic acids that form slowly reversible adducts. The binding kinetics of some of these drugs have either not been well characterized, or have been studied under a variety of different conditions. Read More

    A single amino acid residue regulates the substrate affinity and specificity of indoleamine 2,3-dioxygenase.
    Arch Biochem Biophys 2018 Feb 28;640:1-9. Epub 2017 Dec 28.
    Department of Chemistry, Biology, and Environmental Sciences, Faculty of Science, National University Corporation Nara Women's University, Nara, 630-8506, Japan.
    Indoleamine 2,3-dioxygenase (IDO) is a heme-containing enzyme that catalyses the oxidative cleavage of L-Trp. The ciliate Blepharisma stoltei has four IDO genes (IDO-I, -II, -III and -IV), which seem to have evolved via two sequential gene duplication events. Each IDO enzyme has a distinct enzymatic property, where IDO-III has a high affinity for L-Trp, whereas the affinity of the other three isoforms for L-Trp is low. Read More

    The histidine phosphocarrier protein, HPr, binds to the highly thermostable regulator of sigma D protein, Rsd, and its isolated helical fragments.
    Arch Biochem Biophys 2018 02 27;639:26-37. Epub 2017 Dec 27.
    Instituto de Biocomputación y Física de Sistemas Complejos, Joint Units IQFR-CSIC-BIFI, and GBsC-CSIC-BIFI, Universidad de Zaragoza, Spain; Aragon Institute for Health Research (IIS Aragon), Zaragoza, Spain; Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain; Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Zaragoza, Spain; Fundación ARAID, Diputación General de Aragón, Zaragoza, Spain. Electronic address:
    The phosphotransferase system (PTS) controls the preferential use of sugars in bacteria and it is also involved in other processes, such as chemotaxis. It is formed by a protein cascade in which the first two proteins are general (namely, EI and HPr) and the others are sugar-specific permeases. The Rsd protein binds specifically to the RNA polymerase (RNAP) σfactor. Read More

    Hydrophobic interactions of sucralose with protein structures.
    Arch Biochem Biophys 2018 02 26;639:38-43. Epub 2017 Dec 26.
    Department of Physics, Wesleyan University, Middletown, CT 06457, USA; Molecular Biophysics Program, Wesleyan University, Middletown, CT 06457, USA; Department of Physics, Ripon College, Ripon, WI 54971, USA. Electronic address:
    Sucralose is a commonly employed artificial sweetener that appears to destabilize protein native structures. This is in direct contrast to the bio-preservative nature of its natural counterpart, sucrose, which enhances the stability of biomolecules against environmental stress. We have further explored the molecular interactions of sucralose as compared to sucrose to illuminate the origin of the differences in their bio-preservative efficacy. Read More

    Acceleration of amyloid fibril formation by carboxyl-terminal truncation of human serum amyloid A.
    Arch Biochem Biophys 2018 02 26;639:9-15. Epub 2017 Dec 26.
    Department of Biophysical Chemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan.
    Human serum amyloid A (SAA) is a precursor protein of AA amyloidosis. Although the full-length SAA is 104 amino acids long, the C-terminal-truncated SAA lacking mainly residues 77-104 is predominantly deposited in AA amyloidosis. Nevertheless, the amyloid fibril formation of such truncated forms of human SAA has never been investigated. Read More

    Improved rate of substrate oxidation catalyzed by genetically-engineered myoglobin.
    Arch Biochem Biophys 2018 02 23;639:44-51. Epub 2017 Dec 23.
    Department of Chemistry and Biochemistry, The University of Texas at Arlington, Texas 76019, USA. Electronic address:
    This study showcases the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NHTyr) into its active site, replacing the distal histidine (H64) with NHTyr. In peroxide-shunt assays, this variant exhibits an increased rate of turnover for thioanisole and benzaldehyde oxidation as compared to the wild-type (WT) Mb. Read More

    Implication of altered ubiquitin-proteasome system and ER stress in the muscle atrophy of diabetic rats.
    Arch Biochem Biophys 2018 02 24;639:16-25. Epub 2017 Dec 24.
    Biochemistry Division, National Institute of Nutrition, Hyderabad, India. Electronic address:
    Background: Skeletal muscle is adversely affected in type-1 diabetes, and excessively stimulated ubiquitin-proteasome system (UPS) was found to be a leading cause of muscle wasting or atrophy. The role of endoplasmic reticulum (ER) stress in muscle atrophy of type-1 diabetes is not known. Hence, we investigated the role of UPS and ER stress in the muscle atrophy of chronic diabetes rat model. Read More

    Membrane-damaging activities of mannosylated ovalbumin are involved in its antibacterial action.
    Arch Biochem Biophys 2018 02 19;639:1-8. Epub 2017 Dec 19.
    Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address:
    Mannosylated ovalbumin (Man-OVA) prepared by modification of carboxyl groups with p-aminophenyl α-d-mannopyranoside shows an increase of net positive charge, which may enhance its binding to bacterial membrane. Thus, we aimed to investigate whether Man-OVA exerts antibacterial activity on Escherichia coli and Staphylococcus aureus via membrane-perturbing effect. Man-OVA inhibited the growth of E. Read More

    The alpha helix 1 from the first conserved region of HIV1 gp120 is reconstructed in the short NQ21 peptide.
    Arch Biochem Biophys 2018 01 19;638:66-75. Epub 2017 Dec 19.
    Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory 1-40, Moscow 119991, Russia.
    Investigations of short peptides that can be used in the next phase of synthetic HIV1 vaccine development are an urgent goal, as well as investigations of peptides that can be used in immunological tests with the aim to check the titer of antibodies against the alpha helix 1 from the first conserved region of HIV1 gp120 that are known to cause antibody-dependent cellular cytotoxicity (ADCC). The aim of this work was to study the structure of the NQ21 peptide corresponding to the less mutable part of the first conserved region of HIV1 gp120 (residues 94-114). The NQ21 peptide and its conjugate with biotin (biotin-NQ21) are absolutely alpha-helical in phosphate buffer solutions at pH = 6. Read More

    Intraspecific variation of Centruroides sculpturatus scorpion venom from two regions of Arizona.
    Arch Biochem Biophys 2018 01 16;638:52-57. Epub 2017 Dec 16.
    Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Apartado Postal 510-3, Cuernavaca, Morelos 62210, Mexico. Electronic address:
    This study investigated geographic variability in the venom of Centruroides sculpturatus scorpions from different biotopes. Venom from scorpions collected from two different regions in Arizona; Santa Rita Foothills (SR) and Yarnell (Yar) were analyzed. We found differences between venoms, mainly in the two most abundant peptides; SR (CsEv2e and CsEv1f) and Yar (CsEv2 and CsEv1c) identified as natural variants of CsEv1 and CsEv2. Read More

    Molecular cloning and functional characterization of three terpene synthases from unripe fruit of black pepper (Piper nigrum).
    Arch Biochem Biophys 2018 01 14;638:35-40. Epub 2017 Dec 14.
    Department of Agricultural Biotechnology and Research Institute for Agricultural Sciences, Seoul National University, Seoul 08826, Republic of Korea; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, China. Electronic address:
    To identify terpene synthases (TPS) responsible for the biosynthesis of the sesquiterpenes that contribute to the characteristic flavors of black pepper (Piper nigrum), unripe peppercorn was subjected to the Illumina transcriptome sequencing. The BLAST analysis using amorpha-4,11-diene synthase as a query identified 19 sesquiterpene synthases (sesqui-TPSs), of which three full-length cDNAs (PnTPS1 through 3) were cloned. These sesqui-TPS cDNAs were expressed in E. Read More

    Phosphorylation of Ser-525 in βPix impairs Nox1-activating ability in Caco-2 cells.
    Arch Biochem Biophys 2018 01 11;638:58-65. Epub 2017 Dec 11.
    Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. Electronic address:
    βPix activates Nox1, an O-generating NADPH oxidase, through Rac activation. In this study, we found that S525E mutation of βPix eliminated its Nox1-activating ability in transfected Caco-2 cells. Unexpectedly, affinity for Rac was not diminished but rather enhanced by S525E mutation, and guanine nucleotide exchange factor (GEF) activity was not altered. Read More

    Trichostatin A inhibits deacetylation of histone H3 and p53 by SIRT6.
    Arch Biochem Biophys 2018 01 9;638:8-17. Epub 2017 Dec 9.
    Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, 261 Mountain View Drive, Colchester, VT 05446, USA. Electronic address:
    SIRT6 is an epigenetic modification enzyme that regulates gene transcription through its deacetylase activity. In addition to histone protein, SIRT6 also modify other proteins and enzymes, some of which are central players in metabolic reprogramming and aging process. Therefore, SIRT6 has emerged as a therapeutic target for the treatment of metabolic disorder and age-related diseases. Read More

    Whole length myosin binding protein C stabilizes myosin S2 as measured by gravitational force spectroscopy.
    Arch Biochem Biophys 2018 01 8;638:41-51. Epub 2017 Dec 8.
    Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, TX 76203, USA. Electronic address:
    The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectroscopy (SFS) and gravitational force spectroscopy (GFS). Experiments examined unzipping S2, since it required less force than stretching parallel to the coiled coil. Both GFS and SFS demonstrated that the force required to destabilize the light meromyosin (LMM) was greater than the force required to destabilize the coiled coil at each of three different locations along S2. Read More

    Mutational analysis and dynamic simulation of S-limonene synthase reveal the importance of Y573: Insight into the cyclization mechanism in monoterpene synthases.
    Arch Biochem Biophys 2018 01 8;638:27-34. Epub 2017 Dec 8.
    Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing 100875 China. Electronic address:
    Monoterpene synthases carry out complex reactions to produce multiple products from a sole substrate, geranyl pyrophosphate (GPP). S-limonene synthase (LS) is a model monoterpene synthase that can be explored to understand the catalytic mechanism of these enzymes. In this study, we have identified an active site tyrosine residue (Y573) is crucial for the enzyme activity and mutational analysis indicates that both the aromatic ring and hydroxyl group are essential for the catalysis. Read More

    Interdependence of GLO I and PKM2 in the Metabolic shift to escape apoptosis in GLO I-dependent cancer cells.
    Arch Biochem Biophys 2018 01 8;638:1-7. Epub 2017 Dec 8.
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Genome & Drug Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address:
    Many cancer cells undergo metabolic reprogramming known as the Warburg effect, which is characterized by a greater dependence on glycolysis for ATP generation, even under normoxic conditions. Glyoxalase I (GLO I) is a rate-limiting enzyme involved in the detoxification of cytotoxic methylglyoxal formed in glycolysis and which is known to be highly expressed in many cancer cells. Thus, specific inhibitors of GLO I are expected to be effective anticancer drugs. Read More

    Characterizing interaction forces between actin and proteins of the tropomodulin family reveals the presence of the N-terminal actin-binding site in leiomodin.
    Arch Biochem Biophys 2018 01 6;638:18-26. Epub 2017 Dec 6.
    The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-6515, United States. Electronic address:
    Tropomodulin family of proteins includes several isoforms of tropomodulins (Tmod) and leiomodins (Lmod). These proteins can sequester actin monomers or nucleate actin polymerization. Although it is known that their actin-binding properties are isoform-dependent, knowledge on how they vary in strengths of interactions with G-actin is missing. Read More

    Atorvastatin affects negatively respiratory function of isolated endothelial mitochondria.
    Arch Biochem Biophys 2018 01 5;637:64-72. Epub 2017 Dec 5.
    Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznan, Poland. Electronic address:
    The purpose of this research was to elucidate the direct effects of two popular blood cholesterol-lowering drugs used to treat cardiovascular diseases, atorvastatin and pravastatin, on respiratory function, membrane potential, and reactive oxygen species formation in mitochondria isolated from human umbilical vein endothelial cells (EA.hy926 cell line). Hydrophilic pravastatin did not significantly affect endothelial mitochondria function. Read More

    Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus.
    Arch Biochem Biophys 2018 01 5;637:56-63. Epub 2017 Dec 5.
    Laboratorio "Dr. Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Córdoba, Argentina. Electronic address:
    The aim of this work was to know whether naringin (NA) could prevent the bone complications in a model of streptozotocin (STZ) induced diabetes. Rats were divided in: 1) controls, 2) STZ-rats, 3) STZ-rats treated with 40 mg NA/kg, and 4) STZ-rats treated with 80 mg NA/kg. BMD and BMC were performed by DEXA. Read More

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