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Arch Oral Biol 2023 Sep 16;155:105805. Epub 2023 Sep 16.

Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil; Programa de Pós-Graduação em Ciências - Saúde Bucal da Criança, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - SBFis, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil. Electronic address:

Objective: To investigate the effects of the anticonvulsant valproic acid (VPA) on salivary glands in male rat using biochemical, functional, histomorphometric, and redox state parameters.

Materials And Methods: Twenty-four male Wistar rats were randomly distributed into three groups (n = 8 per group): Control (0.9% saline solution), VPA100 (100 mg/kg), and VPA400 (400 mg/kg). Read More

Arch Oral Biol 2023 Sep 9;155:105803. Epub 2023 Sep 9.

Tokyo Metropolitan Institute for Geriatrics and Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan. Electronic address:

Objective: The masticatory function is intricately linked to several factors like natural teeth count, occlusion, masticatory muscles, and tongue coordination. This study's goal was to formulate a comprehensive masticatory function model, considering sarcopenia's conceptual structure, and subsequently validate its measurement efficacy.

Design: The study encompassed 753 participants (59. Read More

Arch Oral Biol 2023 Sep 16;155:105806. Epub 2023 Sep 16.

Department of Oral Microbiology, School of Dentistry, Chonnam National University, Gwangju, the Republic of Korea. Electronic address:

Objectives: Filifactor alocis is an emerging periodontal pathogen, and macrophage-produced tumor necrosis factor-α (TNF-α) plays important roles in periodontal pathogenesis. In this study, we investigated F. alocis-stimulated TNF-α production in THP-1 macrophagic cells. Read More

Arch Oral Biol 2023 Sep 14;155:105804. Epub 2023 Sep 14.

Department of Odontostomatology, Faculty of Medicine and Health Sciences, University of Barcelona, Campus de Bellvitge, Pavelló de govern, C/Feixa llarga, s/n 08907 L'Hospitalet de Llobregat, Barcelona, Catalonia, Spain; Oral Health and Masticatory System Group (Bellvitge Biomedical Research Institute) IDIBELL, Campus de Bellvitge, C/Feixa llarga, s/n 08907 L'Hospitalet de Llobregat, Barcelona, Catalonia, Spain. Electronic address:

Objective: This cross-sectional study aimed to establish normative values for masticatory side switch (MSS) frequency in young Mexican adults and to assess the relationship between various indices and MSS frequency when masticating different chewing materials.

Design: We enrolled 101 dentate adults and performed four masticatory assays that involved masticating different chewing materials (i.e. Read More

Arch Oral Biol 2023 Sep 6;155:105802. Epub 2023 Sep 6.

Department of Pediatric Dentistry, Institute of Odontology at the Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden. Electronic address:

Objective: This study aims to validate a methodology for analyzing undecalcified, sound dental enamel proteomics using Liquid Chromatography-Mass Spectrometry (LC-MS). The study evaluates various parameters, including the impact of dental root coverage on protein contamination, the efficacy of protease inhibitors during enamel sample preparation, repeatability of LC-MS measurements on dental enamel, and statistical analysis. The study also assesses the effectiveness of combined trypsin and semi-trypsin searches in Mascot for obtaining additional protein identification data. Read More

Arch Oral Biol 2023 Sep 9;155:105801. Epub 2023 Sep 9.

Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao 266001, Shandong Province, China. Electronic address:

Objective: The aim of this study was to explore the correlation between oral microbiota and NLRP3 inflammasome in type 2 diabetes, and to preliminarily explore their possible impact on type 2 diabetes.

Design: The 16S rDNA sequencing technique was used to analyze the microbial composition in the saliva of patients with T2DM and healthy people. Real-time quantitative PCR (RT-qPCR) was used to detect the expression levels of T2DM-related inflammatory cytokines in the blood of two groups. Read More

Arch Oral Biol 2023 Sep 1;155:105800. Epub 2023 Sep 1.

Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu, the Republic of Korea.

Objectives: To investigate whether transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential melastatin 8 (TRPM8) have a function in responding to environmental stimuli in human odontoblast-like cells (hOLCs). Additionally, to explore whether activation of TRPA1 and TRPM8 in hOLCs participates in the regulation of the inflammatory process.

Design: Changes in gene and protein expression levels of TRPA1 and TRPM8 in cultured hOLCs following lipopolysaccharide (LPS) stimulation, which mimics inflammation, were examined using quantitative reverse transcription-polymerase chain reaction and western blot analysis. Read More

Arch Oral Biol 2023 Sep 1;155:105799. Epub 2023 Sep 1.

Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, Brazil. Electronic address:

Objective: Assess risk factors, local and systemic immunological biomarkers in healthy individuals and with Denture Stomatitis (DS).

Design: For this observational transversal study, 27 participants without DS (Group 0), 24 with moderate DS (Group 1), and 25 with severe DS (Group 2) were assessed for sociodemographic, behavioral, and clinical parameters, microbial load of Candida spp., Staphylococcus spp. Read More

Arch Oral Biol 2023 Aug 22;155:105796. Epub 2023 Aug 22.

Department of Endodontics, the Affiliated Hospital of Qingdao University, Qingdao, China; School of Stomatology, Qingdao University, Qingdao, China.

Objective: The aim of this study was to investigate the presence of phosphorylated SMAD2/3 (P-SMAD2/3) in periapical lesions in humans and its possible correlation with matrix metalloproteinase 9 (MMP9) during the development of apical periodontitis.

Design: In this study, a total of 38 samples were collected, consisting of 16 healthy controls and 22 periapical lesions. These samples underwent fixation, dehydration, and embedding for further histologic and immunochemical analysis. Read More

Arch Oral Biol 2023 Aug 26;155:105798. Epub 2023 Aug 26.

Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China. Electronic address:

Objective: Orthodontic tooth movement (OTM) is based on alveolar bone remodeling under mechanical force. In 2010, Piezo1 was identified as a mechanosensitive ion channel that is involved in various physiological functions. We aimed to determine the role of Piezo1 in alveolar bone remodeling during OTM. Read More

Arch Oral Biol 2023 Aug 22;155:105797. Epub 2023 Aug 22.

Department of Orthodontics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Objective: This study aimed to evaluate the role of ruxolitinib in the interferon beta (IFN-β) mediated osteoblast differentiation using human dental pulp stem cells (hDPSCs).

Design: hDPSCs from five deciduous teeth of healthy patients were stimulated by adding human recombinant IFN-β protein (1 or 2 ng/ml) to the osteogenic differentiation induction medium. Substrate formation was determined using Alizarin Red staining, calcium concentration, and osteoblast marker expression levels. Read More

Arch Oral Biol 2023 Aug 21;155:105793. Epub 2023 Aug 21.

Department of Dental Clinic, Stomatology and Oral Pathology Sector, Federal University of Ceara, Fortaleza, Brazil. Electronic address:

Objective: To evaluate the healing potential of Nile tilapia skin collagen using a rat model with experimentally induced traumatic oral ulcers.

Design: Male Wistar rats were segregated into three experimental groups (n = 8/group/euthanasia day). Ulcers were induced using a dermatological punch on the left buccal mucosa. Read More

Arch Oral Biol 2023 Aug 19;155:105794. Epub 2023 Aug 19.

Department of Diagnosis and Surgery, Division of Periodontics, São Paulo State University "Júlio de Mesquita Filho", UNESP, Araçatuba, Brazil; Nucleus of Study and Research in Periodontics and Implantology (NEPPI), School of Dentistry, Sao Paulo, State University (Unesp), Aracatuba, SP, Brazil. Electronic address:

Objectives: To assess sodium alendronate as a local adjunctive therapy for treating experimental periodontitis in male rats treated with chemotherapy.

Design: One-hundred-eighty male rats were randomly divided into two groups (n = 90) based on the systemic treatments: PSS, physiological saline solution; and 5-Fluorouracil, and then, subdivided into three subgroups (n = 30): NT, no treatment; scaling and root planing; and sodium alendronate. Treatments were performed 7 days after induction of experimental periodontitis. Read More

Arch Oral Biol 2023 Aug 20;155:105795. Epub 2023 Aug 20.

Outpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing 100050, China. Electronic address:

Objective: Fibroblast growth factors (FGFs) play pivotal roles in mediating interactions between dental epithelium and mesenchyme throughout tooth initiation and morphogenesis. This study aimed to elucidate the roles of FGF4 and FGF10 in the regulation of tooth development.

Design: In this study, we investigated spatiotemporal expression patterns of FGF4 and FGF10 in the third deciduous molars (DM3) of miniature pigs at the cap, early bell, and late bell stages. Read More

Arch Oral Biol 2023 Aug 7;155:105782. Epub 2023 Aug 7.

Departamento de Antropología, Facultad de Ciencias Sociales, Universidad de Chile, Av. Ignacio Carrera Pinto 1045, Santiago, Chile; Forensic Dentistry Lab, Centro de Investigación en Odontología Legal y Forense -CIO, Facultad de Odontología, Universidad de La Frontera, Temuco, Chile. Electronic address:

Objective: This study assessed the validity of dimensions in posterior dentition for sex estimation in forensic contexts.

Design: A systematic review was established following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). After assessing the risk of bias and methodological quality with the QUADAS-2 system, the data were subjected to statistical tests for a meta-analysis of diagnostic accuracy and I to verify the heterogeneity. Read More

Arch Oral Biol 2023 Aug 16;155:105792. Epub 2023 Aug 16.

Department of Public Health and Forensic Sciences, and Medical Education, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; 1H-TOXRUN, One Health Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, 4585-116 Gandra, Portugal; UCIBIO/REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, No 228, 4050-313 Porto, Portugal. Electronic address:

Objective: This manuscript aims to provide a comprehensive review of the current knowledge in the pathophysiology, diagnosis, prevention, and other relevant clinical and forensic aspects of a potentially severe complication known as medication-related osteonecrosis of the jaw (MRONJ) while synthesizing state-of-the-art information on bisphosphonates and introducing a possible differential diagnosis.

Design: An extensive search was conducted in PubMed (U.S. Read More

Arch Oral Biol 2023 Aug 15;155:105791. Epub 2023 Aug 15.

Department of Oral and Maxillofacial Surgery, Amsterdam Academic Medical Centers and Academic Centre for Dentistry (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.

Objective: Premature dental contact on the fractured side and a contralateral open bite are signs of a unilaterally fractured condyle of the temporomandibular joint (TMJ). The lateral pterygoid muscle pulls the condyle inwards, causing angulation of the fractured part and shortening of the ramus. This imbalance after fracture might change the load in both TMJs and consequently induce remodeling. Read More

Arch Oral Biol 2023 Aug 16;155:105790. Epub 2023 Aug 16.

Department of Stomatology, the Second Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Objectives: This study aims to investigate the role of the long non-coding RNA-AC103563.8 (lncRNA) in promoting oral squamous cell carcinoma (OSCC) development and to conduct preliminary research on its mechanism.

Design: Microarray technology were used to screen out a lncRNA significantly upregulated in OSCC. Read More

Arch Oral Biol 2023 Aug 3;155:105780. Epub 2023 Aug 3.

Division of Cancer Research, Regional Cancer Centre (Research Centre, University of Kerala), Thiruvananthapuram, India. Electronic address:

Objective: To identify the salivary metabolites associated with squamous cell carcinoma of the tongue to develop easy and non-invasive potential biomarkers for disease diagnosis.

Design: Initially, the study utilized untargeted metabolomics to analyze 20 samples of tongue squamous cell carcinoma and 10 control samples. The objective was to determine the salivary metabolites that exhibited differential expression in tongue squamous cell carcinoma. Read More

Arch Oral Biol 2023 Jul 29;155:105778. Epub 2023 Jul 29.

Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Neuroimmune Interface of Pain Research Lab, Campinas, SP, Brazil. Electronic address:

Objective: Semaphorin 4D (Sema4D) is a coupling factor expressed on osteoclasts that may hinder osteoblast differentiation. Since the leukocyte platelet-rich fibrin (L-PRF) membrane promotes growth factor concentration, this study aims to quantify the amount of Sema4D in L-PRF membranes, and analyze the impact of Sema4D on osteoblast cell function in vitro.

Design: Enzyme-linked immunosorbent assay (ELISA) was used to quantify the levels of Sema4D in both L-PRF and whole blood (serum). Read More

Arch Oral Biol 2023 Jul 29;155:105777. Epub 2023 Jul 29.

Research Centre in Evolutionary Anthropology and Palaeoecology, School of Biological and Environmental Sciences, John Moores University, Liverpool L3 3AF, United Kingdom; Ghent University, Department of Archaeology, Sint-Pietersnieuwstraat 35, B-9000 Gent, Belgium.

Objective: Dental pathology and tooth wear data can offer valuable insights into the diet and behaviour of past populations. This study aimed to investigate the presence of dietary continuity by examining different types of dental pathology and tooth wear in a medieval sample from the United Kingdom, comparing them to earlier and later samples from the same location.

Design: A comprehensive examination was conducted on 41 individuals (comprising 914 permanent teeth) retrieved from the medieval cemetery of St. Read More

Arch Oral Biol 2023 Aug 1;155:105779. Epub 2023 Aug 1.

University of Wisconsin, Madison, USA.

Objective: To investigate potential effects of sex on voluntary tongue strength, evoked twitch and tetanic tension, speed of contraction, and muscle fiber cross-sectional area in the muscles of the rat tongue. Additionally, we aimed to determine whether estrous cycle stage impacts any of the dependent variables as a pilot investigation into the use of female rats in a model of tongue exercise and aging.

Design: Fischer 344-Brown Norway male and female rats in two age groups (16 middle-aged, 16 young-adult) were trained to use a tongue force operandum. Read More

Arch Oral Biol 2023 Aug 5;155:105781. Epub 2023 Aug 5.

Department of Endodontics, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China. Electronic address:

Objective: This retrospective study aims to analyze the root canal morphology of permanent mandibular incisors and the bifurcating/merging sites of root canal systems in children aged 9-12 in a Chinese subpopulation.Design Cone-beam computed tomography scans of 976 healthy, untreated and fully developed mandibular incisors were collected. Root canal morphology was identified by Vertucci's classification. Read More

Arch Oral Biol 2023 Oct 27;154:105776. Epub 2023 Jul 27.

Second Clinical Medical College, Lanzhou University, Lanzhou, China; Key Laboratory of Digestive System Tumors of Gansu Province, Lanzhou, China. Electronic address:

Objective: The present study aims to investigate the variations in dental caries (DC) related microbiome abnormality and metabolomics shift in children.

Design: The patients were divided into two groups healthy control (C) and highly affected DC children based on inclusion and exclusion criteria. Saliva samples were collected and used for the taxonomic and functional characterization of oral microbiota. Read More

Arch Oral Biol 2023 Oct 19;154:105775. Epub 2023 Jul 19.

Stomatological Hospital, Southern Medical University, Guangzhou 510280 China. Electronic address:

Objectives: The aim of the present study was to explore the functional role of heparanase (HPSE) and investigate the effect of HPSE on epithelial-mesenchymal transition (EMT) and Tumor-infiltrating activated natural killer cells in oral squamous cell carcinoma (OSCC).

Materials And Methods: human oral squamous carcinoma (SCC-25) cells were transfected with HPSE-specific small interfering RNA. Cell Counting Kit-8 assay was performed to examine cell proliferation, while flow cytometry was performed to analyze the cell cycle. Read More

Arch Oral Biol 2023 Oct 11;154:105765. Epub 2023 Jul 11.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, China; Department of Oral and Maxillofacial Trauma and Temporomandibular Joint Surgery, Hubei-MOST KLOS & KLOBM, School & Hospital of Stomatology, Wuhan University, China. Electronic address:

Objective: This review aims to systematically summarize the methods of establishing various models of trigeminal neuralgia (TN), the scope of application, and current animals used in TN research and the corresponding pain measurements, hoping to provide valuable reference for researchers to select appropriate TN animal models and make contributions to the research of pathophysiology and management of the disease.

Design: The related literatures of TN were searched through PubMed database using different combinations of the following terms and keywords including but not limited: animal models, trigeminal neuralgia, orofacial neuropathic pain. To find the maximum number of eligible articles, no filters were used in the search. Read More

Arch Oral Biol 2023 Oct 11;154:105762. Epub 2023 Jul 11.

Department of Stomatology, the First Medical Center of Chinese PLA General Hospital, No.28 Fuxing Road, Haidian District, Beijing 100853, China. Electronic address:

Objectives: This article aims to elucidate the role of Long non-coding RNA SLC7A11 antisense RNA1 (SLC7A11-AS1) in oral squamous cell carcinoma, which are expected to be useful for the oral squamous cell carcinoma diagnosis and treatment.

Design: SLC7A11-AS1 expression was detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in oral squamous cell carcinoma cell lines. Cellular localization of SLC7A11-AS1C was detected by fluorescence in situ hybridization (FISH) assays and subcellular fractionation assay. Read More

Arch Oral Biol 2023 Oct 11;154:105764. Epub 2023 Jul 11.

Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address:

Objective: We evaluated the effects of eugenol on histological, enzymatic, and oxidative parameters in the pancreas, parotid, submandibular, and sublingual glands of healthy male rats.

Design: Twenty-four adult Wistar rats were assigned into four groups (n = 6/group). Control rats received 2% Tween-20 (eugenol vehicle), whereas the other animals received 10, 20, and 40 mg kg eugenol through gavage daily for 60 d. Read More

Arch Oral Biol 2023 Oct 30;154:105756. Epub 2023 Jun 30.

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objective: The odontogenic differentiation of human dental pulp stem cells (HDPSCs) is associated with reparative dentinogenesis. Transcription factor GATA binding protein 4 (GATA4) is proved to be essential for osteoblast differentiation and bone remodeling. This study clarified the function of GATA4 in HDPSCs odontoblast differentiation. Read More

Arch Oral Biol 2023 Oct 7;154:105763. Epub 2023 Jul 7.

Department of Physiological Science, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil. Electronic address:

Objective: To evaluate the influence of Streptococcus oralis supernatant on the proliferation and virulence of Candida albicans.

Design: S. oralis supernatant was obtained by filtration of overnight cultures. Read More