1,511 results match your criteria Archives Of Insect Biochemistry And Physiology[Journal]


Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.

Arch Insect Biochem Physiol 2020 May 22:e21692. Epub 2020 May 22.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

The southern green stink bug (SGSB, Nezara viridula) is an emerging polyphagous pest in many regions of the world. RNA interference (RNAi) is a valuable method for understanding gene function and holds great potential for pest management. However, RNAi efficiency is variable among insects and the differences in transport of double-stranded RNA (dsRNA) are one of the major factors that contribute to this variability. Read More

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http://dx.doi.org/10.1002/arch.21692DOI Listing

CoFly: A gene coexpression database for the fruit fly Drosophila melanogaster.

Arch Insect Biochem Physiol 2020 May 20:e21693. Epub 2020 May 20.

Department of Bioinformatics, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

The fruit fly Drosophila melanogaster can be used as a model organism for studying various problems in biomedicine and pest management. A large number of fruit fly transcriptomes have been profiled in various cell types, tissues, development stages, toxicological exposures, and other conditions by microarray. Until now, there are still no database developed for exploring those precious data. Read More

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http://dx.doi.org/10.1002/arch.21693DOI Listing

Diel pattern in the structure and function of the gut microbial community in Lymantria dispar asiatica (Lepidoptera: Lymantriidae) larvae.

Arch Insect Biochem Physiol 2020 Jul 14;104(3):e21691. Epub 2020 May 14.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.

In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. Read More

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http://dx.doi.org/10.1002/arch.21691DOI Listing

Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests.

Arch Insect Biochem Physiol 2020 May 11:e21689. Epub 2020 May 11.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

Apoptosis has been widely studied from mammals to insects. Inhibitor of apoptosis (IAP) protein is a negative regulator of apoptosis. Recent studies suggest that iap genes could be excellent targets for RNA interference (RNAi)-mediated control of insect pests. Read More

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http://dx.doi.org/10.1002/arch.21689DOI Listing
May 2020
1.021 Impact Factor

The effect of E93 knockdown on female reproduction in the red flour beetle, Tribolium castaneum.

Arch Insect Biochem Physiol 2020 May 11:e21688. Epub 2020 May 11.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

The E93 transcription factor is a member of helix-turn-helix transcription factor family containing a Pip-squeak motif. This ecdysone primary response gene was identified as a regulator of cell death in Drosophila melanogaster where it is involved in ecdysone-induced autophagy and caspase activity that mediate degeneration of larval tissues during metamorphosis from larva to pupa. However, its function in adult insects is not well studied. Read More

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Development of RNAi methods to control the harlequin bug, Murgantia histrionica.

Arch Insect Biochem Physiol 2020 May 11:e21690. Epub 2020 May 11.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

The harlequin bug (HB), Murgantia histrionica, is a major pest of cabbage family plants throughout its range in the United States. RNA interference (RNAi) is a posttranscriptional gene silencing mechanism that is showing promise as a biopesticide due to the ability to target species-specific genes necessary for growth and/or survival with synthetic double-stranded RNA (dsRNA). In the present study, dsRNA stability assays revealed that nucleases present in the saliva of harlequin bugs did not rapidly degrade dsRNA. Read More

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http://dx.doi.org/10.1002/arch.21690DOI Listing

Chronology of sodium channel mutations associated with pyrethroid resistance in Aedes aegypti.

Arch Insect Biochem Physiol 2020 Jun 6;104(2):e21686. Epub 2020 May 6.

Department of Entomology, Genetics and Neuroscience Programs, Michigan State University, East Lansing, Michigan.

Aedes aegypti is the primary mosquito vector of dengue, yellow fever, Zika and chikungunya. Current strategies to control Ae. aegypti rely heavily on insecticide interventions. Read More

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http://dx.doi.org/10.1002/arch.21686DOI Listing

Double-stranded RNAs targeting inhibitor of apoptosis gene show no significant cross-species activity.

Arch Insect Biochem Physiol 2020 Apr 29:e21683. Epub 2020 Apr 29.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

RNA interference (RNAi) has become an integral part of mainstream research due to its versatility and ease of use. However, the potential nontarget effects associated with double-stranded RNAs (dsRNA) are poorly understood. To explore this, we used dsRNAs targeting the inhibitor of apoptosis (iap) gene from nine insect species and assayed their possible nontarget effects. Read More

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http://dx.doi.org/10.1002/arch.21683DOI Listing

Inhibitor of apoptosis is an effective target gene for RNAi-mediated control of Colorado potato beetle, Leptinotarsa decemlineata.

Arch Insect Biochem Physiol 2020 Apr 29:e21685. Epub 2020 Apr 29.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

The Colorado potato beetle (CPB; Leptinotarsa decemlineata) is one of the most notorious and difficult to control pests of potato and other solanaceous crops in North America. This insect has evolved a remarkable ability to detoxify both plant and synthetic toxins, allowing it to feed on solanaceous plants containing toxic alkaloids and to develop resistance to synthetic chemicals used for its control. RNA interference (RNAi) is a natural mechanism that evolved as an immune response to double-stranded RNA (dsRNA) viruses where dsRNA triggers silencing of target gene expression. Read More

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http://dx.doi.org/10.1002/arch.21685DOI Listing

RNA interference-mediated control of cigarette beetle, Lasioderma serricorne.

Arch Insect Biochem Physiol 2020 Apr 28:e21680. Epub 2020 Apr 28.

Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky.

The cigarette beetle (CB; Lasioderma serricorne) is a pest on many stored products including tobacco. Fumigation is the common control method currently used. However, the options for controlling this pest are limited, due to resistance issues and phasing out of currently used chemical insecticides. Read More

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http://dx.doi.org/10.1002/arch.21680DOI Listing

Noncompetitive tight-binding inhibition of Anticarsia gemmatalis trypsins by Adenanthera pavonina protease inhibitor affects larvae survival.

Arch Insect Biochem Physiol 2020 Jul 27;104(3):e21687. Epub 2020 Apr 27.

Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.

The economic loss in soybean crops caused by the Lepidoptera insects has encouraged the search for new strategies to control this pest, which are currently based on synthetic insecticides. This paper evaluated the ability of ApTI (Adenanthera pavonina trypsin inhibitor) to inhibit trypsin-like proteins from Anticarsia gemmatalis by docking, molecular dynamics, and enzymatic and survival assay. The docking and molecular dynamic simulation between trypsin and ApTI were performed using the program CLUSPRO and NAMD, respectively. Read More

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http://dx.doi.org/10.1002/arch.21687DOI Listing

A journey into the world of insect lipid metabolism.

Arch Insect Biochem Physiol 2020 Jun 25;104(2):e21682. Epub 2020 Apr 25.

Molecular Entomology Laboratory, Department of Plant Protection, Faculty of Agriculture, Ankara University, Ankara, Turkey.

Lipid metabolism is fundamental to life. In insects, it is critical, during reproduction, flight, starvation, and diapause. The coordination center for insect lipid metabolism is the fat body, which is analogous to the vertebrate adipose tissue and liver. Read More

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Plodia interpunctella (Lepidoptera: Pyralidae): Intoxication with essential oils isolated from Lippia turbinata (Griseb.) and analysis of neuropeptides and neuropeptide receptors, putative targets for pest control.

Arch Insect Biochem Physiol 2020 Jul 23;104(3):e21684. Epub 2020 Apr 23.

Laboratorio de Neurobiología de Insectos, Facultad de Ciencias Exactas, Centro Regional de Estudios Genómicos, Universidad Nacional de La Plata, La Plata, Argentina.

The Indian meal moth Plodia interpunctella is a pest of stored products worldwide. Plant-derived essential oils with insecticidal activity could be safe products to control this species. The scarce information about the mode of action of most plant-derived products limits their use for the control of insect pests. Read More

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http://dx.doi.org/10.1002/arch.21684DOI Listing

Influence of dietary aconitine and nicotine on the gut microbiota of two lepidopteran herbivores.

Arch Insect Biochem Physiol 2020 Jul 23;104(3):e21676. Epub 2020 Apr 23.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, China.

The gut microbiota plays an important role in pheromone production, pesticide degradation, vitamin synthesis, and pathogen prevention in the host animal. Therefore, similar to gut morphology and digestive enzyme activity, the gut microbiota may also get altered under plant defensive compound-induced stress. To test this hypothesis, Dendrolimus superans larvae were fed either aconitine- or nicotine-treated fresh leaves of Larix gmelinii, and Lymantria dispar larvae were fed either aconitine- or nicotine-treated fresh leaves of Salix matsudana. Read More

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http://dx.doi.org/10.1002/arch.21676DOI Listing
July 2020
1.021 Impact Factor

Preface.

Authors:
Umut Toprak

Arch Insect Biochem Physiol 2020 Jun 21;104(2):e21681. Epub 2020 Apr 21.

Ankara University, Ankara, Turkey.

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Orally delivered dsRNA induces knockdown of target genes and mortality in the Asian long-horned beetle, Anoplophora glabripennis.

Arch Insect Biochem Physiol 2020 Apr 15:e21679. Epub 2020 Apr 15.

Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky.

The Asian long-horned beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries, including the United States. Methods available to manage or eradicate this pest are extremely limited, but RNA interference (RNAi) technology is a potentially effective method to control ALB. In this study, we used sucrose feeding bioassay for oral delivery of double-strand RNA (dsRNA) to ALB larvae. Read More

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Lipids help double-stranded RNA in endosomal escape and improve RNA interference in the fall armyworm, Spodoptera frugiperda.

Arch Insect Biochem Physiol 2020 Apr 15:e21678. Epub 2020 Apr 15.

Department of Entomology, University of Kentucky, Lexington, Kentucky, 40546, USA.

RNA interference (RNAi) is a valuable method for understanding the gene function and holds great potential for insect pest management. While RNAi is efficient and systemic in coleopteran insects, RNAi is inefficient in lepidopteran insects. In this study, we explored the possibility of improving RNAi in the fall armyworm (FAW), Spodoptera frugiperda cells by formulating dsRNA with Cellfectin II (CFII) transfection reagent. Read More

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Chitosan nanoparticles help double-stranded RNA escape from endosomes and improve RNA interference in the fall armyworm, Spodoptera frugiperda.

Arch Insect Biochem Physiol 2020 Apr 14:e21677. Epub 2020 Apr 14.

Department of Entomology, University of Kentucky, Lexington, Kentucky.

RNA interference (RNAi) is a promising technology for the development of next-generation insect pest control products. Though RNAi is efficient and systemic in coleopteran insects, it is inefficient and variable in lepidopteron insects. In this study, we explored the possibility of improving RNAi in the fall armyworm (FAW), Spodoptera frugiperda by conjugating double-stranded RNA (dsRNA) with biodegradable chitosan (Chi). Read More

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http://dx.doi.org/10.1002/arch.21677DOI Listing

Generation and characterization of inner photoreceptor-specific enhancer-trap lines using a novel piggyBac-Gal4 element in Drosophila.

Arch Insect Biochem Physiol 2020 Jun 13;104(2):e21675. Epub 2020 Apr 13.

Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bogazici University, Istanbul, Turkey.

The Drosophila inner photoreceptors R7 and R8 are responsible for color vision and their differentiation starts at the third instar larval stage. Only a handful of genes with R7 or R8-cell-specific expression are known. We performed an enhancer-trap screen using a novel piggyBac transposable element, pBGay, carrying a Gal4 sequence under the control of the P promoter to identify novel genes expressed specifically in R7 or R8 cells. Read More

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http://dx.doi.org/10.1002/arch.21675DOI Listing

CncC/Maf-mediated xenobiotic response pathway in insects.

Arch Insect Biochem Physiol 2020 Jun 12;104(2):e21674. Epub 2020 Apr 12.

Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky.

Insects have evolved resistance to almost all insecticides developed for their control. Multiple mechanisms of resistance, including enhanced metabolism and excretion of insecticides, target-site insensitivity, reduced penetration of insecticides, and avoidance behavior, have been reported. The genes coding for proteins involved in resistance have been identified in numerous insects. Read More

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Proteome analysis of male accessory gland secretions in Leucinodes orbonalis Guenee (Lepidoptera: Crambidae), a Solanum melongena L. pest.

Arch Insect Biochem Physiol 2020 Jul 30;104(3):e21672. Epub 2020 Mar 30.

Department of Microbiology, Bangalore University, Bengaluru, Karnataka, India.

Male accessory gland (MAG) proteins are transferred along with the sperm to females at the time of mating and have diverse effects on female reproductive physiology in a wide range of insects. In this study, we sought to identify the MAG proteins in Leucinodes orbonalis Guenee, a Solanum melongena L. pest, by analyzing the MAG proteins of virgin and mated male moths by nano-LC-ESI-MS/MS techniques. Read More

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http://dx.doi.org/10.1002/arch.21672DOI Listing

Functional analysis of novel sulfotransferases in the silkworm Bombyx mori.

Arch Insect Biochem Physiol 2020 Jul 29;104(3):e21671. Epub 2020 Mar 29.

Department of Bioscience and Biotechnology, Kyushu University Graduate School, Fukuoka, Japan.

Sulfoconjugation plays a vital role in the detoxification of xenobiotics and in the metabolism of endogenous compounds. In this study, we aimed to identify new members of the sulfotransferase (SULT) superfamily in the silkworm Bombyx mori. Based on amino acid sequence and phylogenetic analyses, two new enzymes, swSULT ST1 and swSULT ST2, were identified that appear to belong to a distinct group of SULTs including several other insect SULTs. Read More

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Aphid transmission of nanoviruses.

Arch Insect Biochem Physiol 2020 Jun 25;104(2):e21668. Epub 2020 Mar 25.

Institute for Epidemiology and Pathogen Diagnostics, Julius Kuehn Institute, Braunschweig, Lower Saxony, Germany.

The genus Nanovirus consists of plant viruses that predominantly infect legumes leading to devastating crop losses. Nanoviruses are transmitted by various aphid species. The transmission occurs in a circulative nonpropagative manner. Read More

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How do toxins from Bacillus thuringiensis kill insects? An evolutionary perspective.

Authors:
David G Heckel

Arch Insect Biochem Physiol 2020 Jun 25;104(2):e21673. Epub 2020 Mar 25.

Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany.

Three-domain Cry toxins from the bacterium Bacillus thuringiensis (Bt) are increasingly used in agriculture to replace chemical insecticides in pest control. Most chemical insecticides kill pest insects swiftly, but are also toxic to beneficial insects and other species in the agroecosystem. Cry toxins enjoy the advantages of high selectivity and the possibility of the application by sprays or transgenic plants. Read More

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sPLA behaves like a prophylactic agent and mediates cellular and humoral immune responses in Plutella xylostella.

Arch Insect Biochem Physiol 2020 Jun 20;104(2):e21670. Epub 2020 Mar 20.

Department of Plant Medicals, Andong National University, Andong, South Korea.

Most immune effectors are inducible to microbial pathogen infection while some are already present to act as prophylactic immunity against as yet unseen infection. This study identified secretory phospholipase A (sPLA ) as a prophylactic factor in diamondback moth, Plutella xylostella. Western blotting using a polyclonal antibody raised against other lepidopteran sPLA reacted specifically with ∼25 kDa protein, which was present at approximately 0. Read More

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Association between olfactory sensitivity and behavioral responses of Drosophila suzukii to naturally occurring volatile compounds.

Arch Insect Biochem Physiol 2020 Jul 19;104(3):e21669. Epub 2020 Mar 19.

Department of Biomedical Sciences, Section of Physiology, University of Cagliari, Cagliari, Italy.

Drosophila suzukii Matsumura (Diptera: Drosophilidae) is an invasive, destructive crop pest that originated in South East Asia. D. suzukii recently invaded Western countries and is threatening both European and American fruit industries. Read More

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Chitin deacetylase: A potential target for Mythimna separata (Walker) control.

Arch Insect Biochem Physiol 2020 May 29;104(1):e21666. Epub 2020 Feb 29.

Department of Plant Protection, College of Agronomy, Northeast Agricultural University, Harbin, China.

Chitin deacetylase (CDA) is a hydrolytic enzyme that modifies chitin into chitosan in the body of insects. In this study, we obtained a full-length complementary DNA sequence (MsCDA1) from the oriental armyworm Mythimna separata by high-throughput sequencing. MsCDA1 is 1,952 bp long and includes 1,620 bp open reading frame encoding 539 amino acids. Read More

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http://dx.doi.org/10.1002/arch.21666DOI Listing
May 2020
1.021 Impact Factor

Targeted somatic mutagenesis through CRISPR/Cas9 ribonucleoprotein complexes in the olive fruit fly, Bactrocera oleae.

Arch Insect Biochem Physiol 2020 Jun 25;104(2):e21667. Epub 2020 Feb 25.

Department of Biology, University of Naples "Federico II", Naples, Italy.

The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), is the most destructive insect pest of olive cultivation, causing significant economic and production losses. Here, we present the establishment of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 methodology for gene disruption in this species. We performed targeted mutagenesis of the autosomal gene white (Bo-we), by injecting into early embryos in vitro preassembled and solubilized Cas9 ribonucleoprotein complexes loaded with two gene-specific single-guide RNAs. Read More

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http://dx.doi.org/10.1002/arch.21667DOI Listing

Identification of the nucleotide exchange factor BmGrpE and its role under high-temperature stress in silkworm, Bombyx mori.

Arch Insect Biochem Physiol 2020 May 24;104(1):e21664. Epub 2020 Feb 24.

School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, China.

The high-temperature stress gene GrpE plays an important role in coping with high-temperature stress. The mutation of key sites of this gene can improve the high-temperature resistance of organisms. In the present study, using complementary DNAs from the silkworm fat body as the template, the open reading frame sequence of the GrpE gene (BmGrpE) was amplified and was found to be 644 bp in length and encode a protein with a predicted molecular weight of 24. Read More

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Knocking down the sex peptide receptor by dsRNA feeding results in reduced oviposition rate in olive fruit flies.

Arch Insect Biochem Physiol 2020 Jun 24;104(2):e21665. Epub 2020 Feb 24.

Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.

Insect pests can cause crop damage in yield or quality, resulting in profit losses for farmers. The primary approach to control them is still the use of chemical pesticides resulting in significant hazards to the environment and human health. Biological control and the sterile insect technique are alternative strategies to improve agriculture protection. Read More

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Transcriptomic analysis at the first instar larval stage of nonmolting Bombyx mori mutant (a42).

Arch Insect Biochem Physiol 2020 May 19;104(1):e21663. Epub 2020 Feb 19.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

The life cycle of the holometabolous insect Bombyx mori (Linnaeus) consists of the embryo, larva, pupa, and adult stages with six larval molts. Ecdysone and juvenile hormones play important roles in the growth and development of the silkworms. The a42 silkworm mutant is recessive and homozygous lethal by exhibiting a dark-colored and small body size and fails to molt to second instar. Read More

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http://dx.doi.org/10.1002/arch.21663DOI Listing

Targeted gene disruption by CRISPR/xCas9 system in Drosophila melanogaster.

Arch Insect Biochem Physiol 2020 May 6;104(1):e21662. Epub 2020 Feb 6.

Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

Although the Cas9 protein from Streptococcus pyogenes (SpCas9) is the most widely used clustered regularly interspaced short palindromic repeats (CRISPR) variant in genome engineering experiments, it does have certain limitations. First, the stringent requirement for the protospacer adjacent motif (PAM) sequence limits the target DNA that can be manipulated using this method in insects. Second, its complementarity specifications are not very stringent, meaning that it can sometimes cause off-target effects at the target site. Read More

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Polycalin is involved in the toxicity and resistance to Cry1Ac toxin in Helicoverpa armigera (Hübner).

Arch Insect Biochem Physiol 2020 May 3;104(1):e21661. Epub 2020 Feb 3.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Polycalin has been confirmed as a binding protein of the Cry toxins in a few Lepidoptera insects, but its function in the action mechanism of Cry1Ac and whether it is involved in resistance evolution are still unclear. In this study, Ligand blot and enzyme-linked immunosorbent assays showed that Helicoverpa armigera polycalin could specifically interact with Cry1Ac with a high affinity (K  = 118.80 nM). Read More

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http://dx.doi.org/10.1002/arch.21661DOI Listing

Sex-biased gene expression in antennae of Drosophila suzukii.

Arch Insect Biochem Physiol 2020 May 29;104(1):e21660. Epub 2020 Jan 29.

Horticultural Crops Research Laboratory, USDA-ARS, Corvallis, Oregon.

Drosophila suzukii differs from other members of the genus Drosophila in its host preference and oviposition behavior. The flies are attracted to ripening fruits, and females have a serrated ovipositor enabling eggs to be laid inside the fruit. In addition to its huge economic impact, its unique chemoecological, morphological, and physiological characteristics have garnered considerable research interests. Read More

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http://dx.doi.org/10.1002/arch.21660DOI Listing

Identification of FerLCH, isolation of ferritin and functional analysis related to interaction with pathogens in Eri-silkworm, Samia cynthia ricini.

Arch Insect Biochem Physiol 2020 May 24;104(1):e21659. Epub 2020 Jan 24.

Department of Science and Technology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, China.

Ferritin is a ubiquitous and conserved iron storage protein that plays a significant role in host detoxification, iron storage, and immune response. Although ferritin has been studied in many species, little is known about its role in the Eri-silkworm (Samia cynthia ricini). In this study, the ferritin light-chain subunit gene, named ScFerLCH, was identified from S. Read More

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http://dx.doi.org/10.1002/arch.21659DOI Listing

Microalgae as a promising and sustainable nutrition source for managed honey bees.

Arch Insect Biochem Physiol 2020 May 24;104(1):e21658. Epub 2020 Jan 24.

Honey Bee Breeding, Genetics and Physiology Research, USDA-ARS, Baton Rouge, Louisiana.

Managed honey bee colony losses are attributed to a number of interacting stressors, but many lines of evidence point to malnutrition as a primary factor. Commercial beekeepers have become increasingly reliant on artificial pollen substitute diets to nourish colonies during periods of forage scarcity and to bolster colony size before pollination services. These artificial diets may be deficient in essential macronutrients (proteins, lipids, prebiotic fibers), micronutrients (vitamins, minerals), and antioxidants. Read More

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http://dx.doi.org/10.1002/arch.21658DOI Listing

Seasonal phenotype-specific expression of microRNAs during metamorphosis in the European map butterfly Araschnia levana.

Arch Insect Biochem Physiol 2020 May 21;104(1):e21657. Epub 2020 Jan 21.

Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany.

The European map butterfly (Araschnia levana) is a well-known example of seasonal polyphenism because the spring and summer imagoes exhibit distinct morphological phenotypes. The day length and temperature during larval and prepupal development determine whether spring or summer imagoes emerge after metamorphosis. Inspired by the fundamentally different transcriptomic profiles in prepupae developing from larvae exposed to long days or short days, we postulate that posttranscriptional epigenetic regulators such as microRNAs (miRNAs) may contribute to the epigenetic control of seasonal polyphenism in A. Read More

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http://dx.doi.org/10.1002/arch.21657DOI Listing

Is the existence of a mutualistic relationship between Lobesia botrana and Botrytis cinerea well-founded?

Arch Insect Biochem Physiol 2020 Apr 17;103(4):e21655. Epub 2020 Jan 17.

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.

Some studies have highlighted benefits for Lobesia botrana by adding Botrytis cinerea mycelium to an artificial larval diet and have suggested a mutualistic relationship between the two organisms on grapevine, hypothesizing that fungal sterols were the nutritional factor involved. Because the nutritional quality of an artificial diet should be similar to grapes to allow extrapolation of the results to the field conditions, in the current study L. botrana larval performance was compared when larvae were fed on grapes (berries) or two artificial diets either with or without enrichment with B. Read More

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Preface.

Authors:

Arch Insect Biochem Physiol 2020 03 16;103(3):e21656. Epub 2020 Jan 16.

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http://dx.doi.org/10.1002/arch.21656DOI Listing

Molecular characterization of DNA methyltransferase 1 and its role in temperature change of armyworm Mythimna separata Walker.

Arch Insect Biochem Physiol 2020 Apr 13;103(4):e21651. Epub 2020 Jan 13.

National Engineering Research Center of Sugarcane, Fujian Agricultural University, Fuzhou, China.

DNA methylation refers to the addition of cytosine residues in a CpG context (5'-cytosine-phosphate-guanine-3'). As one of the most common mechanisms of epigenetic modification, it plays a crucial role in regulating gene expression and in a diverse range of biological processes across all multicellular organisms. The relationship between temperature and DNA methylation and how it acts on the adaptability of migratory insects remain unknown. Read More

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http://dx.doi.org/10.1002/arch.21651DOI Listing

Variation in the pH of experimental diets affects the performance of Lymantria dispar asiatica larvae and its gut microbiota.

Arch Insect Biochem Physiol 2020 Apr 8;103(4):e21654. Epub 2020 Jan 8.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, China.

To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5. Read More

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http://dx.doi.org/10.1002/arch.21654DOI Listing

Effects of terpinen-4-ol fumigation on protein levels of detoxification enzymes in Tribolium confusum.

Arch Insect Biochem Physiol 2020 Apr 20;103(4):e21653. Epub 2019 Dec 20.

Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, China.

Terpinen-4-ol has high fumigating activity to stored-grain pests including Tribolium confusum. To understand the detoxification of terpinen-4-ol in insects, proteomic analysis was performed to identify related proteins and pathways in response to terpinen-4-ol fumigation in T. confusum. Read More

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http://dx.doi.org/10.1002/arch.21653DOI Listing
April 2020
1.021 Impact Factor

Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual.

Arch Insect Biochem Physiol 2020 Mar 17;103(3):e21652. Epub 2019 Dec 17.

Department of Entomology, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward males. In this microreview, we summarize recent advances in synthetic sex ratio distorters based on X-chromosome shredding that can induce male-biased progeny. Read More

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http://dx.doi.org/10.1002/arch.21652DOI Listing

Development of efficient RNAi in Nezara viridula for use in insecticide target discovery.

Arch Insect Biochem Physiol 2020 Mar 13;103(3):e21650. Epub 2019 Dec 13.

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece.

Stink bugs are an emerging pest in many regions of the world but their molecular biology is still poorly understood. While several transcriptomes are available, the lack of validated gene manipulation tools like RNA interference (RNAi) in species such as the southern green stinkbug Nezara viridula precludes the characterization of individual genes in vivo. Such tools are particularly useful in performing high-throughput screens to search for essential genes that can be prioritized as potential insecticide targets. Read More

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http://dx.doi.org/10.1002/arch.21650DOI Listing
March 2020
1.021 Impact Factor

A novel hexamerin with an unexpected contribution to the prophenoloxidase activation system of the Chinese oak silkworm, Antheraea pernyi.

Arch Insect Biochem Physiol 2020 Apr 5;103(4):e21648. Epub 2019 Dec 5.

Department of Biopharmaceutics, School of Life Science and Bio-Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.

Hexamerin was originally identified as a storage protein but later confirmed to be involved in many physiological processes. In the present study, we cloned and characterized a novel hexamerin complementary DNA sequence from the Chinese oak silkworm, Antheraea pernyi (Ap-hexamerin), which shows high homology with reported insect methionine-rich hexamerins. The tissue distribution and time course of expression demonstrated that Ap-hexamerin was predominantly synthesized in the fat body and the expression level was significantly increased in response to the microbial challenge, suggesting the relevance of Ap-hexamerin to immune responses. Read More

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http://dx.doi.org/10.1002/arch.21648DOI Listing

Effects of class-specific, synthetic, and natural proteinase inhibitors on life-history traits of the cotton bollworm Helicoverpa armigera.

Arch Insect Biochem Physiol 2020 Apr 2;103(4):e21647. Epub 2019 Dec 2.

Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany.

Herbivorous insects have more difficulty obtaining proteins from their food than do predators and parasites. The scarcity of proteins in their diet requires herbivores to feed voraciously, thus heavily damaging their host plants. Plants respond to herbivory by producing defense compounds, which reduce insect growth, retard development, and increase mortality. Read More

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http://dx.doi.org/10.1002/arch.21647DOI Listing

The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.

Arch Insect Biochem Physiol 2020 Apr 27;103(4):e21649. Epub 2019 Nov 27.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Acetylation is an important, highly conserved, and reversible post-translational modification of proteins. Previously, we showed by nano-HPLC/MS/MS that many nutrient storage proteins in the silkworm are acetylated. Among these proteins, most of the known 30K proteins were shown to be acetylated, including 23 acetylated 30K proteins containing 49 acetylated sites (Kac), indicating the importance of the acetylation of 30K proteins in silkworm. Read More

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http://dx.doi.org/10.1002/arch.21649DOI Listing

Antifeeding effects of azadirachtin on the fifth instar Spodoptera litura larvae and the analysis of azadirachtin on target sensilla around mouthparts.

Arch Insect Biochem Physiol 2020 Apr 19;103(4):e21646. Epub 2019 Nov 19.

Key Lab of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.

To clarify the types, number, and distribution of sensilla on the head of the fifth instar Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) larvae and identify the main sensilla of azadirachtin acting on larvae, scanning electron microscopy was used to study the morphology of the head and sensilla on the mouthparts. The four sensilla-sensillum basiconicum, sensillum chaeticum, sensillum styloconicum, and sensillum trichodeum-on the head of the fifth instar larvae were treated with 0, 0.1, 0. Read More

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http://dx.doi.org/10.1002/arch.21646DOI Listing

Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci.

Arch Insect Biochem Physiol 2020 Mar 19;103(3):e21645. Epub 2019 Nov 19.

Applied Molecular Microbiology Group, Swansea University School of Medicine, Institute of Life Sciences, Swansea, UK.

In insect reverse genetics, dietary delivery of interfering RNAs is a practical approach in nonmodel species, such as thrips, whose small size, and feeding behavior restricts the use of other delivery methods. In a laboratory context, an unsuitable diet could confound the interpretation of an RNA interference (RNAi) phenotype, however well-formulated artificial diets can minimize experimental variability, reduce the need for insect handling, and can further be used for roles, such as delivering double-strand RNA (dsRNA)-expressing recombinant bacteria. In this study, artificial diets for oral delivery of dsRNA were developed for two important pest thrips species, western flower thrips (Frankliniella occidentalis) and onion thrips (Thrips tabaci), with the goal of (a) stimulating feeding behavior, (b) supporting optimal growth rates of dsRNA-expressing symbiotic bacteria, and (c) nutritionally supporting the thrips for sufficient periods to observe RNAi phenotypes. Read More

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http://dx.doi.org/10.1002/arch.21645DOI Listing

Protection of rice against Nilaparvata lugens by direct toxicity of sodium selenate.

Arch Insect Biochem Physiol 2020 Mar 8;103(3):e21644. Epub 2019 Nov 8.

Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Nilaparvata lugens is one of the most notorious pest insects of cultured rice, and outbreaks of N. lugens cause high economic losses each year. While pest control by chemical pesticides is still the standard procedure for treating N. Read More

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http://dx.doi.org/10.1002/arch.21644DOI Listing