1,584 results match your criteria Archives Of Insect Biochemistry And Physiology[Journal]


Molecular characterization and RNA interference responses of the lethal giant larvae gene in Diabrotica virgifera virgifera adults.

Arch Insect Biochem Physiol 2021 Apr 19:e21787. Epub 2021 Apr 19.

Department of Entomology, Kansas State University, Manhattan, Kansas, USA.

High specificity for silencing target genes and single-copy target genes that yield clear phenotypes are two important factors for the success of RNA interference (RNAi). The lethal giant larvae (Lgl) gene appears to be an ideal gene for RNAi because RNAi can effectively suppress its expression and results in molting defects and mortality in Tribolium castaneum. To investigate the suitability of this gene for RNAi in other insects, we identified and characterized DvLgl from the western corn rootworm, Diabrotica virgifera virgifera, a species exhibiting high RNAi efficiency. Read More

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Transcriptomic analysis of diapause-associated genes in Exorista civilis Rondani (Diptera:Tachinidae).

Arch Insect Biochem Physiol 2021 Apr 16:e21789. Epub 2021 Apr 16.

Research Center for Quality, Safety and Risk Assessment of Grass and Livestock Products, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.

Exorista civilis Rondani (Diptera:Tachinidae) is an excellent dominant parasitic enemy all over the world. But there has been a lack of research on the molecular regulation of diapause in E. civilis. Read More

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Dynamics of heat shock protein responses to thermal stress changes after metamorphosis in a lepidopteran insect.

Arch Insect Biochem Physiol 2021 Apr 16:e21791. Epub 2021 Apr 16.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

In the last decade, unexpected high temperatures have been frequent in spring and early summer. Numerous studies have shown that such thermal stress has substantial effects on life-history traits that influence fitness of insects, but few have examined expression dynamics of heat shock proteins (Hsps) across developmental stages, especially as regards potential carry-over effects at the transcriptional level across metamorphosis. We exposed pupae of the oriental fruit moth ("OFM," Grapholita molesta Busck) to mild heat stress (38°C, 6 h) and then quantified expression patterns of six Hsps (Hsp90, 70, 60, 40, 21, and 11) from pupal through adult stages. Read More

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Induction of diapause by clock proteins period and timeless via changes in PTTH and ecdysteroid titer in the sugar beet moth, Scrobipalpa ocellatella (Lepidoptera: Gelechiidae).

Arch Insect Biochem Physiol 2021 Apr 16:e21790. Epub 2021 Apr 16.

Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

The sugar beet moth, Scrobipalpa ocellatella (Boyd), one of the most severe sugar beet pests, causes quantitative and qualitative yield losses late in the autumn. Previously, it was shown that low temperature and short-day photoperiod together cause diapause induction in pupae. Here, the interaction of the critical elements of the diapause induction, including the period (PER), timeless (TIM), prothoracicotropic hormone (PTTH), and ecdysteroid titer, were investigated. Read More

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Biocidal efficacy of tutin and its influence on immune cells and expression of growth-blocking and neuroglian peptides in Mythimna separata (Lepidoptera: Noctuidae).

Arch Insect Biochem Physiol 2021 Apr 9:e21767. Epub 2021 Apr 9.

College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.

Mythimna separata Walker (Lepidoptera: Noctuidae) is one of the major pests that can cause severe damage to grain crops. The development of low-toxicity and high-performance botanical insecticides is becoming the focus of new pesticide research to control M. separata. Read More

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Cky811 protein expressed by polydnavirus and venom gland of Cotesia kariyai regulates the host Mythimna separata larvae immune response function of C-type lectin responsible for foreign substance recognition which suppresses its melanization and encapsulation.

Arch Insect Biochem Physiol 2021 Apr 5:e21786. Epub 2021 Apr 5.

Faculty of Education, Kogakkan University, Ise City, Mie Prefecture, Japan.

Cotesia kariyai (Ck) larvae implanted into the body cavity of the Mythimna separata (armyworm) larvae get melanized and encapsulated after adhesion by hemocytes called hyperspread cells (HSCs). The present study showed that HSCs could not adhere to the implanted Ck larvae in armyworm larvae after injection of Ck polydnavirus (CkPDV) + venom (V), thus melanization and encapsulation could not occur. A C-type lectin called Mys-IML of the host armyworm larvae was considered to be involved in the recognition of foreign substances which always expressed in hemocytes. Read More

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Melatonin improves cognitive behavior, oxidative stress, and metabolism in tumor-prone lethal giant larvae mutant of Drosophila melanogaster.

Arch Insect Biochem Physiol 2021 Apr 5:e21785. Epub 2021 Apr 5.

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Mutant lethal giant larvae (lgl) flies (Drosophila melanogaster) are known to develop epithelial tumors with invasive characteristics. The present study has been conducted to investigate the influence of melatonin (0.025 mM) on behavioral responses of lgl mutant flies as well as on biochemical indices (redox homeostasis, carbohydrate and lipid metabolism, transaminases, and minerals) in hemolymph, and head and intestinal tissues. Read More

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De novo biosynthesis of linoleic acid is widespread in parasitic wasps.

Arch Insect Biochem Physiol 2021 Apr 4:e21788. Epub 2021 Apr 4.

Institute of Zoology, University of Regensburg, Regensburg, Germany.

Linoleic acid (C18:2 , LA) is an important metabolite with numerous essential functions for growth, health, and reproduction of organisms. It has long been assumed that animals lack ∆12-desaturases, the enzymes needed to produce LA from oleic acid (C18:1 , OA). There is, however, increasing evidence that this is not generally true for invertebrates. Read More

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A switch of microbial flora coupled with ontogenetic niche shift in Leptinotarsa decemlineata.

Arch Insect Biochem Physiol 2021 Mar 16:e21782. Epub 2021 Mar 16.

Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

In Leptinotarsa decemlineata, a final-instar wandering larva typically undergoes an ontogenetic niche shift (ONS), from potato plant during the foraging stage to its pupation site below ground. Using high-throughput sequencing of the bacterial 16S ribosomal RNA gene, we determined the hypothesis that the L. decemlineata pupae harbor stage-specific bacteria to meet the physiological requirements for underground habitat. Read More

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Biochemically identified neuropeptides in a caddisfly (Trichoptera) and a pygmy mole cricket (Orthoptera: Caelifera: Tridactyloidea).

Arch Insect Biochem Physiol 2021 Apr 15;106(4):e21778. Epub 2021 Mar 15.

Department of Biological Sciences, University of Cape Town, Cape Town, South Africa.

One representative of the order Trichoptera, namely the caddisfly Chaetopteryx villosa, was investigated along with the pygmy mole cricket Xya capensis which is a representative of the most basal superfamily of the caeliferan Orthoptera, that is, the Tridactyloidea. From both clades neuropeptides have not been biochemically characterized before this study. Here, members of the adipokinetic hormone family (AKHs) are sequenced via liquid chromatography (LC)-ion trap mass spectrometry from methanolic extracts from the corpora cardiaca of respective species. Read More

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Characterization and functional analysis of a β-adrenergic-like octopamine receptor from the oriental armyworm (Mythimna separata Walker).

Arch Insect Biochem Physiol 2021 Apr 15;106(4):e21772. Epub 2021 Mar 15.

National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.

The β-adrenergic-like octopamine receptor (OA2B2), which binds the biogenic amine octopamine, belongs to the class of G-protein coupled receptors and significantly regulates many physiological and behavioral processes in insects. In this study, the putative open reading frame sequence of the MsOA2B2 gene in Mythimna separata was cloned, the full-length complementary DNA was 1191 bp and it encoded a 396-amino acid protein (GenBank accession number MN822800). Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. Read More

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Bombyx mori can synthesize ascorbic acid through the l-gulose pathway to varying degrees depending on developmental stage.

Arch Insect Biochem Physiol 2021 Apr 15;106(4):e21783. Epub 2021 Mar 15.

School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.

Vitamin C (VC) is an essential nutrient for many animals. However, whether insects, including Bombyx mori, can synthesize VC remains unclear. In this article, the optimized HPLC method was used to determine the content of l-ascorbic acid (AsA) in silkworm eggs, larvae and pupae, and the activity of l-gulono-1,4-lactone oxidase (GULO), a key enzyme in VC synthesis. Read More

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De novo transcriptome assemblies of Epicauta tibialis provide insights into the sexual dimorphism in the production of cantharidin.

Arch Insect Biochem Physiol 2021 Apr 15;106(4):e21784. Epub 2021 Mar 15.

Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, P.R. China.

Blister beetles have medicinal uses for their defensive secretion cantharidin, which has curative effects on many cancers and other diseases. It was demonstrated that sexual dimorphism exists in the production of cantharidin between male and female adults. This study performed a de novo assembly of Epicauta tibialis transcriptomes and analyzed the differentially expressed genes (DEGs) between male and female adults to help to find genes and pathways associated with cantharidin biosynthesis. Read More

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Genetic analysis and transcriptome analysis of the mini mutant of the silkworm, Bombyx mori.

Arch Insect Biochem Physiol 2021 Mar 10:e21774. Epub 2021 Mar 10.

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

The expression levels of some intrinsic genes, protease activity, and regulation of signaling pathways were distinct during different growth and development stages in the silkworm, Bombyx mori. The silkworm mutant mini was discovered from the normal silkworm strain S8V, and the body-size of the mini mutant was smaller than the wild-type from the second-instar and the difference became more significant in the following stages. In this study, genetic analysis of mini mutant showed that mini mutant was controlled by a single recessive gene, manifested as homozygous lethal. Read More

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Apoptotic and autophagic characteristics of perivisceral fat body remodeling of the greater wax moth Galleria mellonella and effects of juvenile hormone analog, fenoxycarb, on these processes.

Arch Insect Biochem Physiol 2021 Mar 9:e21780. Epub 2021 Mar 9.

Biology Department, Science and Literature Faculty, Manisa Celal Bayar University, Manisa, Turkey.

In holometabolous insects, many tissues and organs such as the fat body and midgut undergo a remodeling process during metamorphosis. Larval fat body cells are eliminated by programmed cell death (PCD), while tissue cells that adapt to adult life are formed by stem cells. In this study, we analyzed the features of the remodeling period of Galleria mellonella fat body in terms of PCD types, apoptotic and autophagic cell death characteristics. Read More

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Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect.

Arch Insect Biochem Physiol 2021 Mar 9:e21781. Epub 2021 Mar 9.

College of Agriculture and Life Sciences, Kunming University, Kunming, China.

The Chinese white wax scale insect, Ericerus pela, is an important resource insect in China. The rapid response of E. pela to decreasing temperatures plays key roles in the population distribution. Read More

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shrub is required for spermatogenesis of Drosophila melanogaster.

Arch Insect Biochem Physiol 2021 Apr 3;106(4):e21779. Epub 2021 Mar 3.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology Sciences, School of Life, Central China Normal University, Wuhan, China.

Shrub (CG8055) encodes the vps32/snf7 protein, a filament-forming subunit of the ESCRT (endosomal sorting complexes required for transport)-III complex involved in inward membrane budding. It was reported that shrub was required for abscission in female germline stem cells. In this study, we showed that the expression level of shrub in the testis was significantly higher than that in the ovary of 1-day-old Drosophila melanogaster, suggesting a role in male reproduction. Read More

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Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana.

Arch Insect Biochem Physiol 2021 Apr 3;106(4):e21770. Epub 2021 Mar 3.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, China.

MGST2 is a member of the MAPEG superfamily, which participates in LTC4 synthesis and plays important roles in the regulation of the oxidative stress pathway and some diseases. Here, we isolated a previously uncharacterized gene in Apis cerana cerana named AccMGST2 by reverse transcription-polymerase chain reaction. The biological characteristics of AccMGST2 were analyzed by bioinformatics. Read More

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Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster.

Arch Insect Biochem Physiol 2021 Apr 28;106(4):e21776. Epub 2021 Feb 28.

Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.

Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. Read More

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The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria.

Arch Insect Biochem Physiol 2021 Mar 28;106(3):e21775. Epub 2021 Feb 28.

Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.

DDX3 represents a well-defined subfamily of DEAD-box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. Read More

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Characterization of nuclear localization signal in Ostrinia furnacalis Masculinizer protein.

Arch Insect Biochem Physiol 2021 Mar 28;106(3):e21768. Epub 2021 Feb 28.

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Bombyx mori Masculinizer protein (BmMasc) is essential for both masculinization and dosage compensation in B. mori. We previously identified a bipartite nuclear localization signal (NLS) of BmMasc and two essential residues (lysine at 274 [K274] and arginine at 275 [R275]) implicated in its function. Read More

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Identification of a novel proline-rich antimicrobial protein from the hemolymph of Antheraea mylitta.

Arch Insect Biochem Physiol 2021 Mar 28;106(3):e21771. Epub 2021 Feb 28.

Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.

Antimicrobial proteins (AMPs) are small, cationic proteins that exhibit activity against bacteria, viruses, parasites, fungi as well as boost host-specific innate immune responses. Insects produce these AMPs in the fat body and hemocytes, and release them into the hemolymph upon microbial infection. Hemolymph was collected from the bacterially immunized fifth instar larvae of tasar silkworm, Antheraea mylitta, and an AMP was purified by organic solvent extraction followed by size exclusion and reverse-phase high-pressure liquid chromatography. Read More

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Identification of long noncoding RNAs in silkworm larvae infected with Bombyx mori cypovirus.

Arch Insect Biochem Physiol 2021 Mar 22;106(3):1-12. Epub 2021 Feb 22.

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Bombyx mori cypovirus (BmCPV) is one of the most important pathogens causing severe disease to silkworm. Emerging evidence indicates that long noncoding RNAs (lncRNAs) play importantly regulatory roles in virus infection and host immune response. To better understand the interaction between silkworm, Bombyx mori and BmCPV, we performed a comparative transcriptome analysis on lncRNAs and mRNAs between the virus-infected and noninfected silkworm larvae midgut at two time points postinoculation. Read More

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Mechanism of Metarhizium rileyi evading cellular immune responses in Helicoverpa armigera.

Arch Insect Biochem Physiol 2021 Mar 15;106(3):e21769. Epub 2021 Feb 15.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China.

Upon entry into the hemocoel of host insects, entomopathogenic fungi switch to yeast-like hyphal bodies that are not recognized by host hemocytes and replicate extensively in the hemolymph. The mechanism by which hyphal bodies evade host cellular immunity is not well understood. This study compares Metarhizium rileyi conidia and hyphal bodies with respect to elicitation of the immune response of Helicoverpa armigera and recognition by host pattern recognition receptors (PRRs). Read More

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Transcriptional changes revealed genes and pathways involved in the deficient testis caused by the inhibition of alkaline ceramidase (Dacer) in Drosophila melanogaster.

Arch Insect Biochem Physiol 2021 Mar 15;106(3):e21765. Epub 2021 Feb 15.

State Key Laboratory of Rice Biology, MOA Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, Zhejiang, China.

Sphingolipids are ubiquitous structural components of eukaryotic cell membranes which are vital for maintaining the integrity of cells. Alkaline ceramidase is a key enzyme in sphingolipid biosynthesis pathway; however, little is known about the role of the enzyme in the male reproductive system of Drosophila melanogaster. To investigate the impact of alkaline ceramidase (Dacer) on male Drosophila, we got Dacer deficiency mutants (MUs) and found they displayed apparent defects in the testis's phenotype. Read More

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Three endogenous cellulases from termite, Reticulitermes speratus KMT001.

Arch Insect Biochem Physiol 2021 Mar 15;106(3):e21766. Epub 2021 Feb 15.

Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University, Seoul, Korea.

Among termites, lower termites need symbiotic microorganisms in the digestive tract for digestion and cellulose metabolism. In this symbiotic relationship, the decomposition of cellulose is initiated by endoglucanase in termite salivary glands and completed by β-glycosidase of symbiotic microorganisms in the hindgut. The expression of β-glycosidase in lower termites has been reported in recent studies. Read More

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Protein and phytohormone profiles of Mahanarva spectabilis salivary glands infesting different forages.

Arch Insect Biochem Physiol 2021 Mar 12;106(3):e21773. Epub 2021 Feb 12.

Department of Biochemistry and Molecular Biology, UFV, Laboratory of Enzymology and Biochemistry of Proteins and Peptides, BIOAGRO/INCT - IPP, Viçosa, Minas Gerais, Brazil.

Given the importance of pastures for feeding cattle, the study of factors that affect their productivity is essential to get plant material of higher nutritional quality. Thus, the study of insect-plant interaction is important for the development of control strategies. Pasture spittlebugs affect forage grasses causing severe damage. Read More

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Structure-function analysis of PGRP-S1 from the oriental armyworm, Mythimna separata.

Arch Insect Biochem Physiol 2021 Feb 11;106(2):e21763. Epub 2021 Jan 11.

Anhui Province Key Laboratory of Integrated Pest Management on Crops, Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.

Peptidoglycan recognition proteins (PGRPs) are well known for their abilities to recognize or hydrolyze peptidoglycan (PGN), one of the major bacterial cell wall components. However, much less is known about their antifungal activities. PGRP-S1 was previously identified from a crop pest, Mythimna separata (Walker) (Lepidoptera: Noctuidae). Read More

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February 2021

The validation of the role of several genes related to Bombyx mori nucleopolyhedrovirus infection in vivo.

Arch Insect Biochem Physiol 2021 Feb 8;106(2):e21762. Epub 2021 Jan 8.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Bombyx mori nucleopolyhedrovirus (BmNPV) is one of primary silkworm pathogens and causes a serious damage of cocoon losses every year. Recent years, many works have been done to clarify the silkworm anti-BmNPV mechanism, and a significant progress has been made in screening and studying of genes and proteins related to BmNPV infection, but several of them lacked the proofs in vivo. In this study, to further validate the function of seven newly reported genes in vivo, including BmAtlatin-n, Bmferritin-heavy chain (BmFerHCH), Bmthymosin (BmTHY), Bmseroin1, Bmseroin2, Bmnuclear hormone receptors 96 (BmNHR96), and BmE3 ubiquitin-protein ligase SINA-like 10 (BmSINAL10), the response of them in the midgut, fat body, and hemolymph of differentially resistant strains (resistant strain YeA and susceptible strain YeB) at 48 h following BmNPV infection were analyzed. Read More

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February 2021

Isolation and expression of five genes in the mevalonate pathway of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae).

Arch Insect Biochem Physiol 2021 Feb 24;106(2):e21760. Epub 2020 Nov 24.

College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.

The Chinese white pine beetle Dendroctonus armandi (Tsai and Li) is a significant pest of the Qinling and Bashan Mountains pine forests of China. The Chinese white pine beetle can overcome the defences of Chinese white pine Pinus armandi (Franch) through pheromone-assisted aggregation that results in a mass attack of host trees. We isolated five full-length complementary DNAs encoding mevalonate pathway-related enzyme genes from the Chinese white pine beetle (D. Read More

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February 2021