1,970 results match your criteria Archives Of Andrology[Journal]


The benefits and limitations of the sperm quality analyzer IIB in assessing porcine sperm motility.

Arch Androl 2007 Nov-Dec;53(6):359-65

Reproductive Biology Unit, Perth Zoo, South Perth, Western Australia.

The Sperm Quality Analyzer (SQA) IIB, a member of the SQA-II family of machines which uses the scatter of light by sperm as an indicator of sperm motility, was systematically evaluated as a means of analyzing objectively the motility of porcine epididymal sperm. The sperm motility (%) and the Sperm Motility Index (SMI) are calculated by the machine using pre-programmed algorithms designed for human sperm. The machine performed well and was able to detect changes in sperm motility under experimental conditions. Read More

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An exploratory study of the effects of 12 month administration of the novel long-acting testosterone undecanoate on measures of sexual function and the metabolic syndrome.

Arch Androl 2007 Nov-Dec;53(6):353-7

Bayer-Schering, Department of Men's Healthcare, 13342 Berlin, Germany.

Administration of testosterone undecanoate (TU) over 12 months to men with sexual dysfunction and signs of the metabolic syndrome, restored their plasma testosterone (T) levels to the mid-range of reference values. This had a beneficial effect on their sexual functioning as evidenced by an improvement of their scores on the International Index of Erectile Function. The scores on the Aging Male Symptoms score, AMS, were also improved. Read More

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Effect of sperm preparation techniques by density gradient on intra-individual variation of sperm motility.

Arch Androl 2007 Nov-Dec;53(6):349-51

Division of Reproductive Endocrinology & Infertility, University of Utah School of Medicine, 30 North 1900 East, Suite 2B200, Salt Lake City, Utah 84132, USA.

The purpose of this study was to examine the effect of sperm preparation by density gradient on the intra-individual variation in sperm motility. Patients presenting for density gradient (DG) sperm preparation were analyzed retrospectively. Patients who had more than one preparation were included. Read More

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Spermatoceles in adults: when does size matter?

Arch Androl 2007 Nov-Dec;53(6):345-8

Department of Urology, University of California, San Francisco, 1600 Divisadero St, Box 1695, San Francisco, CA 94143-1695, USA.

Spermatoceles are benign cystic dilations of the epididymis. Despite their relatively common occurrence, it is not clear why or when men want this lesion treated. We present a single institution series of men undergoing spermatocelectomy. Read More

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Testicular microlithiasis in Taiwanese men.

Arch Androl 2007 Nov-Dec;53(6):339-44

Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.

Testicular microlithiasis (TM) is an unusual ultrasonographic manifestation in testicular parenchyma. Limited information is available about TM in Taiwanese men. We performed a retrospective analysis to investigate the characteristics of TM and its association with testicular cancer and infertility in Taiwan. Read More

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Sperm preparation: DNA damage by comet assay in normo- and teratozoospermics.

Arch Androl 2007 Nov-Dec;53(6):325-38

Faculty of Biological Sciences, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

The present study was carried out on semen samples of human fertile and infertile subjects, teratozoospermics (TZs) and idiopathics (IDs), with neat semen and sperm prepared by swim up or Percoll density gradient centrifugation procedures. Sperm morphology analysis revealed that only head and midpiece defects in TZs and IDs were significantly (P < 0.001) higher compared to fertile subjects. Read More

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AZF gene expression analysis in peripheral leukocytes and testicular cells from idiopathic infertility.

Arch Androl 2007 Nov-Dec;53(6):317-24

Department of Urology, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.

The aim of this study was to assess the frequency of AZF microdeletions in peripheral leukocytes and testicular cells in Chinese men with idiopathic infertility. Expression in testicular cells was also determined. In this study, we screened 62 idiopathic infertile patients, in whom karyotype, sperm count and hormonal parameters were evaluated. Read More

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Recovery and cryopreservation of Spanish ibex epididymal spermatozoa.

Arch Androl 2007 Nov-Dec;53(6):309-16

Departamento de Reproducción Animal, SGIT-INIA, Avda. Puerta de Hierro km 5.9, 28040 Madrid, Spain.

A Tris-citric acid-glucose (TCG) diluent containing low concentrations (6%) of egg yolk, and a TCG extender containing lactose (without egg yolk), were compared for use in the cryopreservation of Spanish ibex (Capra pyrenaica) epididymal spermatozoa. To optimize the collection of epididymal spermatozoa, two spermatozoa recovery methods were tested: i) by using small cuts in the cauda epididymides and ii) by the application of air pressure (from a syringe) inside the vas deferens. The percentage of viable spermatozoa recovered was lower (P < 0. Read More

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Human umbilical cord blood serum in culture medium on oocyte maturation In vitro.

Arch Androl 2007 Nov-Dec;53(6):303-7

Center for Reproductive Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei City, Anhui Province, China.

In vitro maturation (IVM) of immature oocytes for infertile patients is an attractive treatment. It can avoid side effects of ovarian stimulation with gonadotropins. However, at the present the successful results of IVM treatment are lower than conventional in vitro fertilization (IVF) treatment. Read More

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Human spermatozoa ultrastructure assessment in the infertility treatment by assisted reproduction technique.

Arch Androl 2007 Nov-Dec;53(6):297-302

Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznań, Poland.

The aim of this study was to prospectively investigate the spermatozoa ultrastructure in relation to the results of in vitro fertilization-embryo transer (IVF-ET). Forty-nine consecutive couples admitted for IVF-ET were prospectively evaluated for electron microscopic spermatozoa morphology and the outcome of IVF-ET. Thirty-four couples revealed successful fertilization, defined as presence of two pronuclei 14-16 hours after spermatozoa administration, while the remaining 15 formed the failure group. Read More

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Seminal vesicle cyst and ipsilateral renal agenesis: laparoscopic approach.

Arch Androl 2007 Sep-Oct;53(5):285-8

Department of Urology and Andrology, West China Hospital, Sichuan University, Chengdu, P. R. China.

Seminal vesicle cysts with ipsilateral renal agenesis is rare. When the patient is symptomatic, surgical treatment may be necessary. However, the seminal vesicle is difficult to access surgically, and current transurethral or open surgical approaches have inherent shortcomings. Read More

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Freezing-free preservation of human spermatozoa--a pilot study.

Arch Androl 2007 Sep-Oct;53(5):275-84

Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI 96822, USA.

This study tested a method for maintaining human spermatozoa without freezing for subsequent use in intracytoplasmic sperm injection (ICSI). We demonstrated that human sperm stored in electrolyte-free solution maintain their motility and viability for at least 4 and 6 weeks, respectively. We also have shown that preserved spermatozoa are fully functional in ICSI. Read More

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Identification of genetic variation in the 5' and 3' non-coding regions of the protamine genes in patients with protamine deregulation.

Arch Androl 2007 Sep-Oct;53(5):267-74

Andrology and IVF Laboratories, Division of Urology, Department of Surgery, School of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.

Deregulation of sperm nuclear protamine ratio (P1/P2) has been shown to correlate with male factor infertility in humans, but the cause of this abnormal protein expression has yet to be identified. Recent studies have shown that there is little genetic variability in the coding regions of either of the protamine gene sequences. However, these studies did not investigate the 5' or 3' non-coding regions of these genes for mutations that might account for changes in the transcriptional or translational regulation of the protamines. Read More

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Crem activator isoforms in normal and impaired human spermatogenesis analyzed by real time RT-PCR.

Arch Androl 2007 Sep-Oct;53(5):257-65

Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.

cAMP responsive element modulator (CREM) activator isoforms are involved in mammalian spermatogenesis and spermiogenesis. CREM proteins are highly expressed in postmeiotic germ cells of rodents and primates. Homozygous CREM inactivated mice exhibit round spermatid maturation arrest. Read More

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Transcripts of aromatase and estrogen receptors and significance of other RNAs in human spermatozoa.

Arch Androl 2007 Sep-Oct;53(5):249-55

Biochemistry Laboratory, University of Caen, France.

The existence of a complex population of mRNAs in human sperm is well documented but their role is not completely elucidated. Evidence for a latent transcriptional capacity and/or a potential translation de novo in mature spermatozoa from fertile men has been provided and is helpful in understanding the final steps of sperm maturation (capacitation and/or the acrosome reaction). Spermatogenesis is controlled by gonadotrophins and testosterone, their effects are modulated by locally-produced factors that include estrogens derived from the irreversible transformation of androgens by aromatase. Read More

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Current assessment of sperm DNA integrity.

Arch Androl 2007 Sep-Oct;53(5):239-47

Center for Human Reproduction, North Shore University Hospital-NYU School of Medicine, New York, USA.

Conventional semen analysis is rapidly losing its place as the gold standard of diagnosis and the cornerstone of treating the infertile male in modern times. Recent technology allows scientists to analyze sperm fertilizing potential and subsequent embryonic growth by studying factors that have previously escaped traditional parameters. It has become increasingly evident that nuclear DNA arrangement is essential to the fertilizing potential of sperm. Read More

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Spermatozoa and chronic treatment with finasteride: a TEM and FISH study.

Arch Androl 2007 Jul-Aug;53(4):229-33

Department of Surgery, Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy.

Finasteride is a specific inhibitor of the 5alpha reductase enzyme originally approved for the treatment of benign prostatic hypertrophy and also for the treatment of androgenetic alopecia (AGA) in men at a dose of 1 mg/day. We report on three cases of young men recruited at our Centre for Male Infertility who had used finasteride for five years. Semen quality was investigated by light microscopy to evaluate sperm concentration and motility. Read More

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October 2007

High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters.

Arch Androl 2007 Jul-Aug;53(4):219-28

Division of Animal Sciences, University of Missouri, 920 E. Campus Drive, Columbia, MO 65211, USA.

The purpose of this study was to investigate the relationship between fertility and quantitative measures of boar semen quality, including various patterns of sperm cytoplasmic droplet (CD) retention, as determined by high power differential interference contrast (DIC) microscopy. A total of 116 ejaculates were collected from a nucleus herd of 18 Large White boars over an eight month period. Semen quality parameters were analyzed for each ejaculate by calculating the percentage of normal spermatozoa, spermatozoa possessing a CD in the proximal, distal, or distal midpiece reflex position, total spermatozoa with an attached cytoplasmic droplet, spermatozoa with non-CD related aberrations and total spermatozoa with abnormalities. Read More

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October 2007

Evidence of Alu and B1 expression in dbEST.

Arch Androl 2007 Jul-Aug;53(4):207-18

Asia Pacific Bioinformatics Research Institute, Honolulu, HI, USA.

Alus and B1s are short interspersed repeat elements (SINEs) derived from the 7SL RNA gene. Alus and B1s exist in the cytoplasm as non-coding RNA indicating that they are actively transcribed, but their function, if any, is unknown. Transcription of individual SINEs is a prerequisite for retroposition, but it is also possible that individual Alu and B1 elements have some cellular functions. Read More

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October 2007

Quantitative assessment of transition proteins 1, 2 spermatid-specific linker histone H1-like protein transcripts in spermatozoa from normozoospermic and asthenozoospermic men.

Arch Androl 2007 Jul-Aug;53(4):199-205

Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznań, Poland.

Spermatid-specific linker histone H1-like protein (HILS1), transition proteins 1 and 2 (TNP1 and TNP2), and protamines 1 and 2 (PRM1 and PRM2) contribute to considerable dense packing of spermatid chromatin during spermiogenesis. We evaluated the HILS1, TNP1, and TNP2 transcript levels in spermatozoa isolated from normozoospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n = 70) and asthenozoospermic (n = 100) donors were purified by centrifugation through a discontinuous Percoll density gradient. Read More

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October 2007

In vivo gene transfer into testis and sperm: developments and future application.

Arch Androl 2007 Jul-Aug;53(4):187-97

Department of Pharmacology, University of Oxford, Oxford, UK.

Despite significant advances in the treatment of infertility via assisted reproductive technology (ART), the underlying causes of idiopathic male infertility still remain unclear. Accumulating evidence suggests that disorders associated with testicular gene expression may play an important role in male infertility. To be able to fully study the molecular mechanisms underlying spermatogenesis and fertilization, it is necessary to manipulate gene expression in male germ cells. Read More

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October 2007

Clinical and radiological evaluation of ejaculatory duct obstruction.

Arch Androl 2007 Jul-Aug;53(4):179-86

Department of Radiology, Kovancilar Government Hospital, Kovancilar, Elazýg-Turkey.

Ejaculatory duct obstruction (EDO) is a rare but surgically correctable cause of male infertility. With the advent and increased use of transrectal ultrasonography and magnetic resonance imaging, abnormalities of the ejaculatory duct (ED) related to infertility have been diagnosed more frequently. Recently, with the increased awareness of functional obstruction of ED, reports have been focusing on the diagnosis of partial or functional EDO. Read More

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October 2007

Role(s) of the serine/threonine protein phosphatase 1 on mammalian sperm motility.

Arch Androl 2007 Jul-Aug;53(4):169-77

Department of Obstetrics and Gynecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong, SAR, China.

Mammalian spermatozoa acquire the capacity for motility and fertilization during the transit through the epididymis under the control of different factors, such as cAMP, intracellular pH, intracellular calcium and phosphorylation of sperm proteins. As the acquisition of functional competence including gaining motility during epididymal transit occurs in the complete absence of contemporaneous gene transcription and translation on the part of the spermatozoa, it is widely accepted that post-translational modifications are the only means by which spermatozoa can acquire functionality. Serine-threonine protein phosphatase 1 (PP1) together with their testis/sperm-specific interacting proteins might be involved in this regulatory mechanism. Read More

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October 2007

The preparation of human spermatozoal RNA for clinical analysis.

Arch Androl 2007 May-Jun;53(3):161-7

Wayne State University School of Medicine, Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Institute for Scientific Computing, Detroit, MI 48201, USA.

The recent identification of RNA as a component of mature spermatozoa necessitated the development of a reliable isolation protocol capable of yielding a high-quality substrate. In addition to the inherent difficulties associated with isolating RNA, the procedure as applied to sperm must overcome the resilient nature and reduced RNA content found within this cell type. Further, the protocol must be suited to the clinical setting. Read More

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Analysis of sperm karyotypes in a patient treated with griseofulvin.

Arch Androl 2007 May-Jun;53(3):157-60

Department of Medical Genetics, University of Calgary, Calgary, Alberta, Canada.

Griseofulvin is known to interfere with chromosome segregation by binding to microtubule-associated proteins. Studies in mouse germ cells have demonstrated that griseofulvin can induce aneuploidy (numerical chromosome abnormalities) at therapeutic concentrations. The aim of this study was to determine if chronic griseofulvin treatment led to an increased frequency of sperm chromosome abnormalities in one male subject. Read More

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Association between age and epididymal and accessory sex gland function and their relation to sperm motility.

Authors:
Saad Elzanaty

Arch Androl 2007 May-Jun;53(3):149-56

Scanian Andrology Centre, Fertility Centre, Malmö University Hospital, Lund University, Malmö, Sweden.

Increased male age has been associated with significant reduction in pregnancy rates. This study investigated the association between age, the function of epididymal and accessory sex glands, and their relation to sperm motility. Ejaculates from 498 men assessed for infertility were analysed according to WHO [1999] guidelines. Read More

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Pre-pubertal di(2-ethylhexyl) phthalate (DEHP) exposure of young boars did not affect sperm in vitro penetration capacity of homologous oocytes post-puberty.

Arch Androl 2007 May-Jun;53(3):141-7

Department of Clinical Sciences, Division of Reproduction, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Di(2-ethylhexyl) phthalate (DEHP), a plastic softener used in polyvinylchloride (PVC) products (e.g., plastic bags and medical equipment), has been reported to have toxic effects on animal reproduction and is considered an environmental hazard based, mostly, on rodent studies. Read More

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Function of the sperm nuclear matrix.

Arch Androl 2007 May-Jun;53(3):135-40

Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii. Honolulu, HI 96822, USA.

Mammalian spermatozoa contain some of the most highly compact chromatin. This is due to the DNA binding proteins, the protamines, which replace most of the histones during spermiogenesis. This chromatin, however, shares some features with somatic cell chromatin. Read More

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Genetic risk factors in male infertility.

Arch Androl 2007 May-Jun;53(3):125-33

Andrology Unit, Department of Clinical Physiopathology, Viale Pieraccini, 6 Firenze, Italy.

The etiopathogenesis of testicular failure remains unknown in about half of the cases and is referred to as "idiopathic infertility". "Idiopathic" testicular failure is of probable genetic origin since the number of genes involved in human spermatogenesis is likely thousands and only a small proportion of them have been identified and screened in infertile men. In parallel with studies aimed to identify mutations with a clear cause-effect relationship in spermatogenesis candidate genes, there is an increasing interest towards genetic susceptibility factors to male infertility. Read More

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