Search our Database of Scientific Publications and Authors

I’m looking for a

    11372 results match your criteria Applied microbiology and biotechnology[Journal]

    1 OF 228

    Correction to: Prokaryotic expression and action mechanism of antimicrobial LsGRP1C recombinant protein containing a fusion partner of small ubiquitin-like modifier.
    Appl Microbiol Biotechnol 2017 Nov 22. Epub 2017 Nov 22.
    Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei, 10617, Taiwan, Republic of China.
    The published online version contains editing mistake in Table 2. See below for the corrected Table. Read More

    Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli.
    Appl Microbiol Biotechnol 2017 Nov 20. Epub 2017 Nov 20.
    Research Division Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, TU Wien, Vienna, Austria.
    Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous biopharmaceutically important proteins are currently produced as IBs. To obtain correctly folded, soluble product, IBs have to be processed, namely, harvested, solubilized, and refolded. Several years ago, it was discovered that, depending on cultivation conditions and protein properties, IBs contain partially correctly folded protein structures, which makes IB processing more efficient. Read More

    Extensive hydrolysis of raw rice starch by a chimeric α-amylase engineered with α-amylase (AmyP) and a starch-binding domain from Cryptococcus sp. S-2.
    Appl Microbiol Biotechnol 2017 Nov 20. Epub 2017 Nov 20.
    Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, Hefei, 230601, Anhui, People's Republic of China.
    Recombinant chimeric α-amylase (AmyP-Cr) was constructed by a catalytic core of α-amylase (AmyP) from a marine metagenomic library and a starch-binding domain (SBDCr) of α-amylase from Cryptococcus sp. S-2. The molecular fusion did not alter optimum pH, optimum temperature, hydrolysis products, and an ability of preferential and rapid degradation towards raw rice starch, but catalytic efficiency and thermostability were remarkably improved compared with those of the wild-type AmyP. Read More

    KfoA, the UDP-glucose-4-epimerase of Escherichia coli strain O5:K4:H4, shows preference for acetylated substrates.
    Appl Microbiol Biotechnol 2017 Nov 20. Epub 2017 Nov 20.
    Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Science, Shandong University, Jinan, 250012, China.
    Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This polysaccharide, commonly referred to as K4CP, is a potentially important source of precursors for chemoenzymatic or bioengineering synthesis of chondroitin sulfate. KfoA, encoded by a gene from region 2 of the K4 capsular gene cluster, shows high homology to the UDP-glucose-4-epimerase (GalE) from E. Read More

    Correction to: Optimized synthesis of novel prenyl ferulate performed by feruloyl esterases from Myceliophthora thermophila in microemulsions.
    Appl Microbiol Biotechnol 2017 Nov 20. Epub 2017 Nov 20.
    Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 97187, Luleå, Sweden.
    After publication of the original article, authors found that there has been a minor mistake in the units of kcat and kcat/Km in Table 2. The units should be 10(3) min(-1) g(-1) FAE for kcat and mM(-1) min(-1) g(-1) FAE for kcat/Km. This correction does not affect any conclusions drawn within the article. Read More

    Enrichment of lignocellulose-degrading microbial communities from natural and engineered methanogenic environments.
    Appl Microbiol Biotechnol 2017 Nov 19. Epub 2017 Nov 19.
    Department of Environmental Engineering, Faculty of Civil Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
    The aim of this study was to develop an effective bioaugmentation concept for anaerobic digesters treating lignocellulosic biomass such as straw. For that purpose, lignocellulose-degrading methanogenic communities were enriched on wheat straw from cow and goat rumen fluid as well as from a biogas reactor acclimated to lignocellulosic biomass (sorghum as mono-substrate). The bacterial communities of the enriched cultures and the different inocula were examined by 454 amplicon sequencing of 16S rRNA genes while the methanogenic archaeal communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the mcrA gene. Read More

    Fluorescent pseudomonads pursue media-dependent strategies to inhibit growth of pathogenic Verticillium fungi.
    Appl Microbiol Biotechnol 2017 Nov 18. Epub 2017 Nov 18.
    Institute of Microbiology and Genetics and Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Grisebachstraße 8, 37077, Goettingen, Germany.
    Verticillium species represent economically important phytopathogenic fungi with bacteria as natural rhizosphere antagonists. Growth inhibition patterns of Verticillium in different media were compared to saprophytic Aspergillus strains and were significantly more pronounced in various co-cultivations with different Pseudomonas strains. The Brassica napus rhizosphere bacterium Pseudomonas fluorescens DSM8569 is able to inhibit growth of rapeseed (Verticillium longisporum) or tomato (Verticillium dahliae) pathogens without the potential for phenazine or 2,4-diacetylphloroglucinol (DAPG) mycotoxin biosynthesis. Read More

    Tolerance improvement of Corynebacterium glutamicum on lignocellulose derived inhibitors by adaptive evolution.
    Appl Microbiol Biotechnol 2017 Nov 18. Epub 2017 Nov 18.
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
    Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the industrial fermenting strain Corynebacterium glutamicum S9114 was evolved for improved inhibitor tolerance using long-term adaptive evolution by continuously transferring into the inhibitors containing corn stover hydrolysate every 24 h, and finally a stably evolved C. glutamicum was obtained after 128 days of serial transfers. Read More

    Enhanced catalytic efficiency and enantioselectivity of epoxide hydrolase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis for (R)-epichlorohydrin synthesis.
    Appl Microbiol Biotechnol 2017 Nov 18. Epub 2017 Nov 18.
    Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
    Enantioselective hydrolysis of epoxides by epoxide hydrolase (EH) is one of the most attractive approaches for the synthesis of chiral epoxides. So far, attempts to develop an efficient epoxide hydrolase -mediated biotransformation have been limited by either the low activity or insufficient enantioselectivity of epoxide hydrolase. In this study, iterative saturation mutagenesis (ISM) of epoxide hydrolase from Agrobacterium radiobacter AD1 (ArEH) was performed for efficient production of (R)-epichlorohydrin. Read More

    Guidelines to reach high-quality purified recombinant proteins.
    Appl Microbiol Biotechnol 2017 Nov 18. Epub 2017 Nov 18.
    CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal.
    The final goal in recombinant protein production is to obtain high-quality pure protein samples. Indeed, the successful downstream application of a recombinant protein depends on its quality. Besides production, which is conditioned by the host, the quality of a recombinant protein product relies mainly on the purification procedure. Read More

    Simultaneous detection of NADPH consumption and H2O2 production using the Ampliflu™ Red assay for screening of P450 activities and uncoupling.
    Appl Microbiol Biotechnol 2017 Nov 17. Epub 2017 Nov 17.
    Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487, Greifswald, Germany.
    Cytochrome P450s belong to a large and diverse group of heme-containing enzymes. These monooxygenases catalyze the incorporation of a single atom of molecular oxygen into their substrate. In contrast to most other enzymes, the activity of P450 enzymes is not only dependent on substrate and cofactor availability and reaction conditions, but also depends on the coupling efficiency of the catalytic cycle itself. Read More

    Production of arabitol from enzymatic hydrolysate of soybean flour by Debaryomyces hansenii fermentation.
    Appl Microbiol Biotechnol 2017 Nov 17. Epub 2017 Nov 17.
    Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325, USA.
    Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Read More

    A guard-killer phage cocktail effectively lyses the host and inhibits the development of phage-resistant strains of Escherichia coli.
    Appl Microbiol Biotechnol 2017 Nov 17. Epub 2017 Nov 17.
    College of Veterinary Medicine, Jilin University, Xi'an Street 5333#, Changchun, 130062, Jilin, People's Republic of China.
    In recent years, after the emergence of a large number of multidrug-resistant bacteria, phages and phage-associated products for the prevention and control of bacterial disease have revealed prominent advantages as compared with antibiotics. However, bacteria are susceptible to becoming phage-resistant, thus severely limiting the application of phage therapy. In this study, Escherichia coli cells were incubated with lytic bacteriophages to obtain mutants that were resistant to the lytic phages. Read More

    Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation.
    Appl Microbiol Biotechnol 2017 Nov 17. Epub 2017 Nov 17.
    Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia.
    Malolactic fermentation (MLF) is an important step in winemaking, which can be notoriously unreliable due to the fastidious nature of Oenococcus oeni. This study aimed to use directed evolution (DE) to produce a more robust strain of O. oeni having the ability to withstand high ethanol concentrations. Read More

    Novel polycondensed biopolyamide generated from biomass-derived 4-aminohydrocinnamic acid.
    Appl Microbiol Biotechnol 2017 Nov 17. Epub 2017 Nov 17.
    Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
    Biomass plastics are expected to contribute to the establishment of a carbon-neutral society by replacing conventional plastics derived from petroleum. The biomass-derived aromatic amine 4-aminocinnamic acid (4ACA) produced by recombinant bacteria is applied to the synthesis of high-performance biopolymers such as polyamides and polyimides. Here, we developed a microbial catalyst that hydrogenates the α,β-unsaturated carboxylic acid of 4ACA to generate 4-aminohydrocinnamic acid (4AHCA). Read More

    Enzymatic properties of β-N-acetylglucosaminidases.
    Appl Microbiol Biotechnol 2017 Nov 16. Epub 2017 Nov 16.
    Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.
    β-N-Acetylglucosaminidases (GlcNAcases) hydrolyse N-acetylglucosamine-containing oligosaccharides and proteins. These enzymes produce N-acetylglucosamine (GlcNAc) and have a wide range of promising applications in the food, energy, and pharmaceutical industries, such as synergistic degradation of chitin with endo-chitinases and using GlcNAc to produce sialic acid, bioethanol, single-cell proteins, and pharmaceutical therapeutics. GlcNAcases also play an important role in the dynamic balance of cellular O-linked GlcNAc levels, catabolism of ganglioside storage in Tay-Sachs disease, and bacterial cell wall recycling and flagellar assembly. Read More

    RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles.
    Appl Microbiol Biotechnol 2017 Nov 16. Epub 2017 Nov 16.
    IBET, Instituto de Biologia Experimental e Tecnológica, 2780-901, Oeiras, Portugal.
    Conformationally complex membrane proteins (MPs) are therapeutic targets in many diseases, but drug discovery has been slowed down by the lack of efficient production tools. Co-expression of MPs with matrix proteins from enveloped viruses is a promising approach to obtain correctly folded proteins at the surface of virus-like particles (VLPs), preserving their native lipidic environment. Here, we implemented a site-specific recombinase-mediated cassette exchange (RMCE) strategy to establish a reusable HIV-1 Gag-expressing insect cell line for fast production of target MPs on the surface of Gag-VLPs. Read More

    Spatial separation of photosynthesis and ethanol production by cell type-specific metabolic engineering of filamentous cyanobacteria.
    Appl Microbiol Biotechnol 2017 Nov 15. Epub 2017 Nov 15.
    Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
    Cyanobacteria, which perform oxygenic photosynthesis, have drawn attention as hosts for the direct production of biofuels and commodity chemicals from CO2 and H2O using light energy. Although cyanobacteria capable of producing diverse chemicals have been generated by metabolic engineering, anaerobic non-photosynthetic culture conditions are often necessary for their production. In this study, we conducted cell type-specific metabolic engineering of the filamentous cyanobacterium Anabaena sp. Read More

    Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles.
    Appl Microbiol Biotechnol 2017 Nov 15. Epub 2017 Nov 15.
    Vilnius University Life Sciences Center Institute of Biotechnology, Sauletekio al. 7, 10257, Vilnius, Lithuania.
    Hepatitis E is a globally distributed human disease caused by hepatitis E virus (HEV). In Europe, it spreads through undercooked pork meat or other products and with blood components through transfusions. There are no approved or golden standard serologic systems for HEV diagnostics. Read More

    Metabolic synergies in the biotransformation of organic and metallic toxic compounds by a saprotrophic soil fungus.
    Appl Microbiol Biotechnol 2017 Nov 14. Epub 2017 Nov 14.
    Laboratorio Biodiversità dei Funghi, Dipartimento di Biologia Ambientale, Sapienza Università di Roma, 00185, Rome, Italy.
    The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil containing high concentrations of HCH isomers and potentially toxic elements (Pb, V). Read More

    Improvement of biosynthesis and accumulation of bioactive compounds by elicitation in adventitious root cultures of Polygonum multiflorum.
    Appl Microbiol Biotechnol 2017 Nov 14. Epub 2017 Nov 14.
    Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
    We examined the effects of abiotic (methyl jasmonate [MeJA] and salicylic acid [SA]) and biotic (yeast extract and chitosan) elicitors for improvement of bioactive compounds production on adventitious root cultures in Polygonum multiflorum. The application of yeast extract resulted in significantly (p ≤ 0.05) higher dry root biomass (9. Read More

    Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete.
    Appl Microbiol Biotechnol 2017 Nov 14. Epub 2017 Nov 14.
    School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton, New Zealand.
    Self-healing mechanisms are a promising solution to address the concrete cracking issue. Among the investigated self-healing strategies, the biotechnological approach is distinguished itself by inducing the most compatible material with concrete composition. In this method, the potent bacteria and nutrients are incorporated into the concrete matrix. Read More

    Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris.
    Appl Microbiol Biotechnol 2017 Nov 14. Epub 2017 Nov 14.
    Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux AgroBio Tech, Avenue de la Faculté, 2B, B-5030, Gembloux, Belgium.
    The methylotrophic yeast Komagataella (Pichia) pastoris has become one of the most utilized cell factories for the production of recombinant proteins over the last three decades. This success story is linked to its specific physiological traits, i.e. Read More

    Influence of polyaluminum chloride on microbial characteristics in anaerobic membrane bioreactors for sludge digestion.
    Appl Microbiol Biotechnol 2017 Nov 14. Epub 2017 Nov 14.
    State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084, People's Republic of China.
    In this study, two parallel lab-scale anaerobic membrane bioreactors (AnMBRs), one of which was dosed with polyaluminum chloride (PAC) for membrane fouling control, were operated for treating excess activated sludge collected from a wastewater treatment plant (WWTP). The AnMBRs were inoculated with anaerobic digested sludge collected from an anaerobic digester of another WWTP. The microbial community of digested sludge and cake layer in AnMBRs, as well as that of excess sludge, was analyzed through polymerase chain reaction coupled with denaturing gradient gel electrophoresis (PCR-DGGE) and Illumina MiSeq. Read More

    Whole-cell-dependent biosynthesis of sulfo-conjugate using human sulfotransferase expressing budding yeast.
    Appl Microbiol Biotechnol 2017 Nov 13. Epub 2017 Nov 13.
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0351, Japan.
    Cytosolic sulfotransferases (SULTs), one of the predominant phase II drug metabolizing enzymes (DME), play important roles in metabolism of xeno- and endobiotics to generate their sulfo-conjugates. These sulfo-conjugates often have biological activities but are difficult to study, because even though only small amounts are required to evaluate their efficacy and safety, chemical or biological synthesis of sulfo-conjugatesis is often challenging. Previously, we constructed a DME expression system for cytochrome P450 and UGT, using yeast cells, and successfully produced xenobiotic metabolites in a whole-cell-dependent manner. Read More

    Type IV pilus biogenesis genes and their roles in biofilm formation in the biological control agent Lysobacter enzymogenes OH11.
    Appl Microbiol Biotechnol 2017 Nov 13. Epub 2017 Nov 13.
    College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, No.1 Weigang, Nanjing City, Jiangsu Province, 210095, People's Republic of China.
    Type IV pilus (T4P) is widespread in bacteria, yet its biogenesis mechanism and functionality is only partially elucidated in a limited number of bacterial species. Here, by using strain OH11 as the model organism, we reported the identification of 26 T4P structural or functional component (SFC) proteins in the Gram-negative Lysobacter enzymogenes, which is a biocontrol agent potentially exploiting T4P-mediated twitching motility for antifungal activity. Twenty such SFC coding genes were individually knocked-out in-frame to create a T4P SFC deletion library. Read More

    Optimization and application of a DNA-launched infectious clone of equine arteritis virus.
    Appl Microbiol Biotechnol 2017 Nov 13. Epub 2017 Nov 13.
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin, 150069, People's Republic of China.
    Reverse genetics is one of the most powerful tools in modern virology. Equine arteritis virus (EAV) is the prototype member of the Equartevirus. In this study, a new reverse genetics system for the recovery of equine arteritis virus from a cDNA plasmid, which contains viral cDNA sequence flanked by hammerhead ribozyme (HamRz) and hepatitis delta virus ribozyme (HdvRz) sequences in both terminals of the viral genome, was developed by optimization of the promoter and terminator regions. Read More

    Production of D-arabitol from D-xylose by the oleaginous yeast Rhodosporidium toruloides IFO0880.
    Appl Microbiol Biotechnol 2017 Nov 11. Epub 2017 Nov 11.
    Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL, 61801, USA.
    The sugar alcohol D-arabitol is one of the Department of Energy's top twelve bio-based building block chemicals. In this study, we found that the oleaginous yeast Rhodosporidium toruloides IFO0880 produces D-arabitol during growth on xylose in nitrogen-rich medium. Efficient xylose utilization was a prerequisite for extracellular D-arabitol production. Read More

    Recent advances in microbial production of aromatic natural products and their derivatives.
    Appl Microbiol Biotechnol 2017 Nov 10. Epub 2017 Nov 10.
    College of Engineering, The University of Georgia, 146 Riverbend Research South, Athens, GA, 30602, USA.
    Aromatic natural products represent a diverse class of chemicals with great industrial significance. Usually, they serve as the building blocks for production of various polymers, esters, fibers, nutraceuticals, and pharmaceuticals. However, industrial synthesis of aromatic natural products still relies heavily on petroleum-based chemical processes, which are not sustainable and eco-friendly. Read More

    Semi-rational engineering of CYP153A35 to enhance ω-hydroxylation activity toward palmitic acid.
    Appl Microbiol Biotechnol 2017 Nov 9. Epub 2017 Nov 9.
    School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics and Institute of Bioengineering, Seoul National University, Seoul, South Korea.
    CYP153A35 from Gordonia alkanivorans was recently characterized as fatty acid ω-hydroxylase. To enhance the catalytic activity of CYP153A35 toward palmitic acid, site-directed saturation mutagenesis was attempted using a semi-rational approach that combined structure-based computational analysis and subsequent saturation mutagenesis. Using colorimetric high-throughput screening (HTS) method based on O-demethylation activity of P450, CYP153A35 D131S and D131F mutants were selected. Read More

    Response of microbial communities to pesticide residues in soil restored with Azolla imbricata.
    Appl Microbiol Biotechnol 2017 Nov 8. Epub 2017 Nov 8.
    Faculty of Civil Engineering and Architecture, Zhejiang University of Technology, Hangzhou, 310014, China.
    Under conditions of Azolla imbricata restoration, the high-throughput sequencing technology was employed to determine change trends of microbial community structures in the soil that had undergone long-term application of pesticides. The relationship between the content of pesticide residues in the soil and the microbial community structure was analyzed. The results indicated that the microbial diversity was strongly negatively correlated with the contents of pesticide residues in the soil. Read More

    Retrieved 16S rRNA and nifH sequences reveal co-dominance of Bradyrhizobium and Ensifer (Sinorhizobium) strains in field-collected root nodules of the promiscuous host Vigna radiata (L.) R. Wilczek.
    Appl Microbiol Biotechnol 2017 Nov 6. Epub 2017 Nov 6.
    National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Faisalabad, Pakistan.
    In the present study, the relative distribution of endophytic rhizobia in field-collected root nodules of the promiscuous host mung bean was investigated by sequencing of 16S ribosomal RNA (rRNA) and nifH genes, amplified directly from the nodule DNA. Co-dominance of the genera Bradyrhizobium and Ensifer was indicated by 32.05 and 35. Read More

    Identification of tomato miRNAs responsive to root colonization by endophytic Pochonia chlamydosporia.
    Appl Microbiol Biotechnol 2017 Nov 5. Epub 2017 Nov 5.
    Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via G. Amendola 122/D, 70126, Bari, Italy.
    The molecular mechanisms active during the endophytic phase of the fungus Pochonia chlamydosporia are still poorly understood. In particular, few data are available on the links between the endophyte and the root response, as modulated by noncoding small RNAs. In this study, we describe the microRNAs (miRNAs) that are differentially expressed (DE) in the roots of tomato, colonized by P. Read More

    Environmental factors shaping the archaeal community structure and ether lipid distribution in a subtropic river and estuary, China.
    Appl Microbiol Biotechnol 2017 Nov 4. Epub 2017 Nov 4.
    Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China.
    Archaea are widespread and abundant in aquatic and terrestrial habitats and play fundamental roles in global biogeochemical cycles. Archaeal lipids, such as isoprenoid glycerol diakyl glycerol tetraethers (iGDGTs), are important biomarkers tracing changes in archaeal community structure and biogeochemical processes in nature. However, the linkage between the archaeal populations and the GDGT distribution in the natural environment is poorly examined, which hindered the application and interpretation of GDGT-based climate or environmental proxies. Read More

    Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets.
    Appl Microbiol Biotechnol 2017 Nov 4. Epub 2017 Nov 4.
    Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland.
    The DNA coding sequence of TaqStoffel polymerase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. Read More

    Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids.
    Appl Microbiol Biotechnol 2017 Nov 4. Epub 2017 Nov 4.
    The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China.
    XynB from Aspergillus niger ATCC1015 (AnXynB) is a mesophilic glycoside hydrolase (GH) family 11 xylanase which holds great potentials in a wide variety of industrial applications. In the present study, the catalytic activity and stability of AnXynB were improved by a combination of computational and experimental approaches. Virtual mutation and molecular dynamics simulations indicated that the introduction of Glu and Asn altered the interaction network at the - 3 subsite. Read More

    Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines.
    Appl Microbiol Biotechnol 2017 Nov 4. Epub 2017 Nov 4.
    Grup d'Enginyeria Cel·lular i Bioprocés, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona, Spain.
    Transient gene expression (TGE) has been used at small and medium scale for the production of biologicals in sufficient quantities to perform pre-clinical and characterization studies. Polyethyleneimine (PEI)-mediated transfection offers a low toxicity and non-expensive method for cell transfection. DNA and PEI concentration for transient gene expression has been extensively optimized in order to increase product titers. Read More

    Deep sequencing analysis of transcriptomes in Aspergillus oryzae in response to salinity stress.
    Appl Microbiol Biotechnol 2017 Nov 3. Epub 2017 Nov 3.
    Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang, 330013, China.
    Characterization of the changes after various stimuli is crucial to comprehend the adaptation of cells to the changing condition. Aspergillus oryzae is widely used for the industrial production of soy sauce, which always encounter changes within a complex environment, such as salinity stress. However, the protective biochemical mechanisms of A. Read More

    Enhanced production of perylenequinones in the endophytic fungus Shiraia sp. Slf14 by calcium/calmodulin signal transduction.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
    Perylenequinones (PQ) that notably produce reactive oxygen species upon exposure to visible light are a class of photoactivated polyketide mycotoxins produced by fungal plant pathogens such as Shiraia sp. The involvement of Ca(2+)/calmodulin (CaM) signalling in PQ biosynthesis was investigated by submerged culturing of Shiraia sp. Slf14, a species that produces hypocrellins HA and HB and elsinochromes EA, EB, and EC. Read More

    Microbial diversity and composition in different gut locations of hyperlipidemic mice receiving krill oil.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    School of Marine Science, Ningbo University, Ningbo, 315211, China.
    Low-dose (LD, 100 mg kg(-1) day(-1)), moderate-dose (MD, 200 mg kg(-1) day(-1)), and high-dose (HD, 600 mg kg(-1) day(-1)) krill oil treatments have a stepwise, enhanced effect on alleviating hyperlipidemia, and 16S rRNA sequencing of the fecal samples demonstrates that krill oil treatment alters microbial communities. Feces may not represent all microbial communities in the gastrointestinal (GI) tract. Therefore, in this study, the stored ileal and colon samples collected from LD and HD groups were sequenced, and the location-specific modulations of microbial communities were observed after krill oil treatments. Read More

    Rational design for heterologous production of aurovertin-type compounds in Aspergillus nidulans.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
    Aurovertins are the structurally diverse polyketides that distribute widely in different fungal species. They feature a 2,6-dioxabicyclo[3.2. Read More

    Difference of nitrogen-cycling microbes between shallow bay and deep-sea sediments in the South China Sea.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology 3-211, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, Shanghai, 200240, China.
    In marine sediments, microorganisms are known to play important roles in nitrogen cycling; however, the composition and quantity of microbes taking part in each process of nitrogen cycling are currently unclear. In this study, two different types of marine sediment samples (shallow bay and deep-sea sediments) in the South China Sea (SCS) were selected to investigate the microbial community involved in nitrogen cycling. The abundance and composition of prokaryotes and seven key functional genes involved in five processes of the nitrogen cycle [nitrogen fixation, nitrification, denitrification, dissimilatory nitrate reduction to ammonium (DNRA), and anaerobic ammonia oxidation (anammox)] were presented. Read More

    Distinguishing between metabolically active and dormant bacteria on paper.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, USA.
    Switching between metabolically active and dormant states provides bacteria with protection from environmental stresses and allows rapid growth under favorable conditions. This rapid growth can be detrimental to the environment, e.g. Read More

    Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    Noda Institute for Scientific Research, 399 Noda, Noda City, 278-0037, Japan.
    The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. The result implied that SclR is potentially important in both traditional fermentative manufacturing and commercial enzyme production in A. Read More

    Variations in Monascus pigment characteristics and biosynthetic gene expression using resting cell culture systems combined with extractive fermentation.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
    Monascus pigments are promising sources of natural food colorants, and their productivity can be improved by a novel extractive fermentation technology. In this study, we investigated the variations in pigment characteristics and biosynthetic gene expression levels in resting cell culture systems combined with extractive fermentation in Monascus anka GIM 3.592. Read More

    Nisin-induced expression of recombinant T cell epitopes of major Japanese cedar pollen allergens in Lactococcus lactis.
    Appl Microbiol Biotechnol 2017 Nov 2. Epub 2017 Nov 2.
    School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami, Kochi, 782-8502, Japan.
    Japanese cedar pollinosis is a seasonal allergic disease caused by two major pollen allergens: Cry j 1 and Cry j 2 antigens. To develop an oral vaccine to treat pollinosis, we constructed recombinant Lactococcus lactis harboring the gene encoding fused T cell epitopes from the Cry j 1 and Cry j 2 antigens. The recombinant T cell epitope peptide was designed to contain the fused cholera toxin B subunit as an adjuvant and a FLAG tag at the C-terminus. Read More

    Recombinant Lactobacillus plantarum expressing HA2 antigen elicits protective immunity against H9N2 avian influenza virus in chickens.
    Appl Microbiol Biotechnol 2017 Dec 31;101(23-24):8475-8484. Epub 2017 Oct 31.
    College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
    Low pathogenic H9N2 subtype avian influenza virus (AIV) can lead to moderate respiratory symptoms and low egg production rates in poultry. Due to its immunologic suppression, other various infectious pathogens give rise to the co-infection of hosts, causing heavy economic losses in the commercial poultry industry in both China and worldwide. Therefore, it is time to explore a novel, safe, and effective vaccine. Read More

    Enhanced long-chain fatty alcohol oxidation by immobilization of alcohol dehydrogenase from S. cerevisiae.
    Appl Microbiol Biotechnol 2017 Oct 31. Epub 2017 Oct 31.
    School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile.
    This work reports on the oxidation of long-chain aliphatic alcohols catalyzed by a stabilized alcohol dehydrogenase from S. cerevisiae (yeast alcohol dehydrogenase (YADH)). In particular, the oxidation of the fatty alcohol tetracosanol (C24H50O) to yield lignoceric acid (C23H47COOH) was studied. Read More

    Substrate specificity and membrane topologies of the iron-containing ω3 and ω6 desaturases from Mortierella alpina.
    Appl Microbiol Biotechnol 2017 Oct 30. Epub 2017 Oct 30.
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
    Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to protect against inflammatory diseases and enhance brain development and function. This had led to a marked increase in demand for fish and fish oils in human diets, supplements, and aquaculture and created a need for new, sustainable n-3 LC-PUFA sources. Read More

    Decay of the water reed Phragmites communis caused by the white-rot fungus Phlebia tremellosa and the influence of some environmental factors.
    Appl Microbiol Biotechnol 2017 Oct 30. Epub 2017 Oct 30.
    Department of Microbiology, University of Greifswald, Greifswald, Germany.
    The strain Phlebia tremellosa SBUG 1630 isolated from a thatched roof in Northern Germany is capable of colonizing and degrading effectively the water reed Phragmites communis. Within 96 h after inoculation, mycelia covered both the outer and the inner surface of reed shoot fragments as observed by scanning electron microscopy. Interestingly, top culm sections and culm edges were particularly susceptible towards fungal degradation. Read More

    1 OF 228