13,488 results match your criteria Applied microbiology and biotechnology[Journal]


A comparative study of microbial community and functions of type 2 diabetes mellitus patients with obesity and healthy people.

Appl Microbiol Biotechnol 2020 Jul 4. Epub 2020 Jul 4.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.

The gut microbiota is crucial in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the metabolism of T2DM patients is not well-understood. We aimed to identify the differences on composition and function of gut microbiota between T2DM patients with obesity and healthy people. Read More

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http://dx.doi.org/10.1007/s00253-020-10689-7DOI Listing

Quorum sensing signal autoinducer-2 promotes root colonization of Bacillus velezensis SQR9 by affecting biofilm formation and motility.

Appl Microbiol Biotechnol 2020 Jul 4. Epub 2020 Jul 4.

Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.

Root colonization of beneficial rhizobacteria is critical for their beneficial effects. Quorum sensing (QS) has been reported to affect the colonization of many plant pathogens. However, how QS signals regulate root colonization of beneficial rhizobacteria is unclear. Read More

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http://dx.doi.org/10.1007/s00253-020-10713-wDOI Listing

Moving away from traditional antibiotic treatment: can macrocyclic lactones from marine macroalga-associated heterotroph be the alternatives?

Appl Microbiol Biotechnol 2020 Jul 3. Epub 2020 Jul 3.

Crustacean Fisheries Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.

Intertidal red algae Hypnea valentiae associated Bacillus amyloliquefaciens MTCC 12716 revealed potential inhibitory effects on the growth of drug-resistant pathogens. In the genome of B. amyloliquefaciens MTCC 12716, biosynthetic gene clusters encoding antibacterial metabolites were predicted, which might be expressed and contributed to the broad-spectrum anti-infective activity. Read More

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http://dx.doi.org/10.1007/s00253-020-10658-0DOI Listing

Leveraging nature's biomolecular designs in next-generation protein sequencing reagent development.

Appl Microbiol Biotechnol 2020 Jul 2. Epub 2020 Jul 2.

Institute for Bioscience and Biotechnology Research (IBBR), National Institute of Standards and Technology (NIST) and the University of Maryland (UMD), 9600 Gudelsky Drive, Rockville, MD, 20850, USA.

Next-generation approaches for protein sequencing are now emerging that could have the potential to revolutionize the field in proteomics. One such sequencing method involves fluorescence-based imaging of immobilized peptides in which the N-terminal amino acid of a polypeptide is readout sequentially by a series of fluorescently labeled biomolecules. When selectively bound to a specific N-terminal amino acid, the NAAB (N-terminal amino acid binder) affinity reagent identifies the amino acid through its associated fluorescence tag. Read More

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http://dx.doi.org/10.1007/s00253-020-10745-2DOI Listing

Production of the anticancer drug taxol by the endophytic fungus Epicoccum nigrum TXB502: enhanced production by gamma irradiation mutagenesis and immobilization technique.

Appl Microbiol Biotechnol 2020 Jul 2. Epub 2020 Jul 2.

Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt.

Taxol, a phyto-extracted diterpenoid, is the most commercially needed drug in cancer chemotherapy. In spite of the microbial production of taxol being successful and prospective, the reported yields are still not sufficient for large-scale production. Thus, the discovery of new taxol-producing microbial strains and production enhancement methodologies such as process optimization, strain improvement, and immobilization technique are the main objectives. Read More

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http://dx.doi.org/10.1007/s00253-020-10712-xDOI Listing

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant.

Appl Microbiol Biotechnol 2020 Jun 30. Epub 2020 Jun 30.

Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands.

Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. Read More

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http://dx.doi.org/10.1007/s00253-020-10748-zDOI Listing

Expression of enzymes for 3'-phosphoadenosine-5'-phosphosulfate (PAPS) biosynthesis and their preparation for PAPS synthesis and regeneration.

Appl Microbiol Biotechnol 2020 Jun 29. Epub 2020 Jun 29.

Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.

The synthesis of sulfated polysaccharides involves the sulfation of simpler polysaccharide substrates, through the action sulfotransferases using the cofactor, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Three enzymes are essential for the in vitro synthesis of PAPS, namely, pyrophosphatase (PPA), adenosine 5'-phosphosulfate kinase (APSK), and ATP sulfurylase (ATPS). The optimized enzyme expression ratio and effect on PAPS synthesis were evaluated using ePathBrick, a novel synthetic biology tool that assemble multiple genes in a single vector. Read More

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http://dx.doi.org/10.1007/s00253-020-10709-6DOI Listing

Mannitol: physiological functionalities, determination methods, biotechnological production, and applications.

Appl Microbiol Biotechnol 2020 Jun 29. Epub 2020 Jun 29.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Mannitol is a naturally occurring six-carbon sugar alcohol that has wide applications in the food and pharmaceutical industry because of its many properties, namely being a natural sweetener with a low metabolism and no glycemic index. The increasing demand for mannitol has spurred many studies of its production. Compared with its chemical synthesis and extraction from plants, both of which are difficult to satisfy for industrial requirements, biotechnological production of mannitol has received considerably more attention and interest from scientists because of its known advantages over those two methods. Read More

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http://dx.doi.org/10.1007/s00253-020-10757-yDOI Listing

Production and excretion of astaxanthin by engineered Yarrowia lipolytica using plant oil as both the carbon source and the biocompatible extractant.

Appl Microbiol Biotechnol 2020 Jun 29. Epub 2020 Jun 29.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA.

This study aimed to develop a bioprocess using plant oil as the carbon source for lipid-assimilating yeast to produce high-value astaxanthin. Using high-oleic safflower oil as a model, efficient cell growth and astaxanthin production by the engineered Yarrowia lipolytica strain ST7403 was demonstrated, and a considerable portion of astaxanthin was found excreted into the spent oil. Astaxanthin was the predominant carotenoid in the extracellular oil phase that allowed facile in situ recovery of astaxanthin without cell lysis. Read More

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http://dx.doi.org/10.1007/s00253-020-10753-2DOI Listing

Production of L-alanyl-L-glutamine by immobilized Escherichia coli expressing amino acid ester acyltransferase.

Appl Microbiol Biotechnol 2020 Jun 27. Epub 2020 Jun 27.

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.

Production of Ala-Gln by the E. coli expressing α-amino acid ester acyltransferase was a promising technical route due to its high enzyme activity, but the continuous production ability still needs to improve. Therefore, the immobilized E. Read More

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http://dx.doi.org/10.1007/s00253-020-10752-3DOI Listing

Increased campesterol synthesis by improving lipid content in engineered Yarrowia lipolytica.

Appl Microbiol Biotechnol 2020 Jun 26. Epub 2020 Jun 26.

Engineering Research Center of High Value Utilization of Western China Fruit Resources, Ministry of Education, Xi'an, 710119, People's Republic of China.

Sterols attract increasing attention due to their important bioactivities. The oleaginous yeast Yarrowia lipolytica has large lipid droplets, which provide storage for the accumulated steroid compounds. In this study, we have successfully constructed a campesterol biosynthetic pathway by modifying the synthetic pathway of ergosterol in Y. Read More

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http://dx.doi.org/10.1007/s00253-020-10743-4DOI Listing

Targeted gene deletion in Brettanomyces bruxellensis with an expression-free CRISPR-Cas9 system.

Appl Microbiol Biotechnol 2020 Jun 26. Epub 2020 Jun 26.

The Australian Wine Research Institute, PO Box 197, Glen Osmond, Adelaide, SA, 5064, Australia.

The ability to genetically manipulate microorganisms has been essential for understanding their biology and metabolism. Targeted genome editing relies on highly efficient homologous recombination, and while this is readily observed in the yeast Saccharomyces cerevisiae, most non-conventional yeast species do not display this trait and remain recalcitrant to targeted editing methods. CRISPR-based editing can bypass the requirement for high levels of native homologous recombination, enabling targeted modification to be more broadly implemented. Read More

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http://dx.doi.org/10.1007/s00253-020-10750-5DOI Listing

The sug operon involves in resistance to quaternary ammonium compounds in Listeria monocytogenes EGD-e.

Appl Microbiol Biotechnol 2020 Jun 25. Epub 2020 Jun 25.

Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.

In recent years, an increasing number of Listeria monocytogenes strains with resistance to quaternary ammonium compounds (QACs) have been reported. However, the genetic basis for QACs resistance in L. monocytogenes remains poorly understood. Read More

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http://dx.doi.org/10.1007/s00253-020-10741-6DOI Listing

An overview and future prospects on aptamers for food safety.

Appl Microbiol Biotechnol 2020 Jun 25. Epub 2020 Jun 25.

Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.

Introduction: Many bacteria are responsible for infections in humans and plants, being found in vegetables, water, and medical devices. Most bacterial detection methods are time-consuming and take days to give the result. Aptamers are a promising alternative for a quick and reliable measurement technique to detect bacteria present in food products. Read More

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http://dx.doi.org/10.1007/s00253-020-10747-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315907PMC

Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures.

Appl Microbiol Biotechnol 2020 Jun 23. Epub 2020 Jun 23.

The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P. O. Box 309#, Shanghai, 200237, China.

Tyrosine (Tyr) is crucial to the maintenance of the monoclonal antibody (mAb) titers and quality attributes in fed-batch cultures of recombinant Chinese hamster ovary (rCHO) cells. However, the relation between tyrosine and these aspects is not yet fully defined. In order to further elucidate such a relation, two groups of fed-batch experiments with high tyrosine (H-T) or low tyrosine (L-T) additions producing an IgG1 monoclonal antibody against CD20 were implemented to investigate the intracellular and extracellular effects of tyrosine on the culture performance. Read More

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http://dx.doi.org/10.1007/s00253-020-10744-3DOI Listing

Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungus Aureobasidium pullulans.

Appl Microbiol Biotechnol 2020 Jun 23. Epub 2020 Jun 23.

Geomicrobiology Group, School of Life Sciences, University of Dundee, Dundee, Scotland, DD1 5EH, UK.

Aureobasidium pullulans is a ubiquitous and widely distributed fungus in the environment, and exhibits substantial tolerance against toxic metals. However, the interactions between metals and metalloids with the copious extracellular polymeric substances (EPS) produced by A. pullulans and possible relationships to tolerance are not well understood. Read More

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http://dx.doi.org/10.1007/s00253-020-10732-7DOI Listing

Identification of a unique 1,4-β-D-glucan glucohydrolase of glycoside hydrolase family 9 from Cytophaga hutchinsonii.

Appl Microbiol Biotechnol 2020 Jun 23. Epub 2020 Jun 23.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, People's Republic of China.

Cytophaga hutchinsonii is an aerobic cellulolytic soil bacterium that rapidly digests crystalline cellulose. The predicted mechanism by which C. hutchinsonii digests cellulose differs from that of other known cellulolytic bacteria and fungi. Read More

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http://dx.doi.org/10.1007/s00253-020-10731-8DOI Listing

Genomic and transcriptomic perspectives on mycoremediation of polycyclic aromatic hydrocarbons.

Appl Microbiol Biotechnol 2020 Jun 22. Epub 2020 Jun 22.

Department of Biotechnology, Graduate School and Institute of Life Science and Natural Resources, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, South Korea.

Mycoremediation holds great potential in remedying toxic environments contaminated with polyaromatic organic pollutants. To harness the natural process for practical applications, understanding the genetic and molecular basis of the remediation process is prerequisite. Compared to known bacterial degradation pathways of aromatic pollutants, however, the fungal degradation system is less studied and understanding of the genetic basis for biochemical activity is still incomplete. Read More

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http://dx.doi.org/10.1007/s00253-020-10746-1DOI Listing

Green synthesis of multipurpose carbon quantum dots from red cabbage and estimation of their antioxidant potential and bio-labeling activity.

Appl Microbiol Biotechnol 2020 Jun 22. Epub 2020 Jun 22.

Department of Bio-nanotechnology, Gachon University, Gyeonggi-do, 13120, Republic of Korea.

We present a green synthesis of fluorescent carbon quantum dots (CQDs) by using red cabbage (rc) and a one-step hydrothermal approach. The rcCQDs were characterized by various techniques such as UV-visible spectroscopy, transmission electron microscopy (TEM), high-resolution TEM, Fourier-transform infrared spectroscopy, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy. The synthesized rcCQDs exhibited an average size of 3 nm, measured by TEM, blue fluorescence, and a quantum yield of 8. Read More

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http://dx.doi.org/10.1007/s00253-020-10726-5DOI Listing

A natural occurring bifunctional CPD/(6-4)-photolyase from the Antarctic bacterium Sphingomonas sp. UV9.

Appl Microbiol Biotechnol 2020 Jun 22. Epub 2020 Jun 22.

Biochemistry and Molecular Biology, Faculty of Sciences, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay.

Photolyases are flavoproteins that repair ultraviolet-induced DNA lesions (cyclobutane pyrimidine dimer or CPD, and pyrimidine (6-4) pyrimidone photoproducts or (6-4)-PPs), using blue light as an energy source. These enzymes are substrate specific, meaning that a specific photolyase repairs either a CPD or a (6-4)-PP. In this work, we produced a class II CPD-photolyase (called as PhrSph98) from the Antarctic bacterium Sphingomonas sp. Read More

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http://dx.doi.org/10.1007/s00253-020-10734-5DOI Listing

Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals.

Appl Microbiol Biotechnol 2020 Jun 21. Epub 2020 Jun 21.

Chair of Chemistry of Biogenic Resources, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Schulgasse 16, 94315, Straubing, Germany.

2-keto-3-L-arabinonate dehydratase (L-KdpD) and 2-keto-3-D-xylonate dehydratase (D-KdpD) are the third enzymes in the Weimberg pathway catalyzing the dehydration of respective 2-keto-3-deoxy sugar acids (KDP) to α-ketoglutaric semialdehyde (KGSA). The Weimberg pathway has been explored recently with respect to the synthesis of chemicals from L-arabinose and D-xylose. However, only limited work has been done toward characterizing these two enzymes. Read More

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http://dx.doi.org/10.1007/s00253-020-10742-5DOI Listing

Recent progress in metabolic engineering of microbial formate assimilation.

Appl Microbiol Biotechnol 2020 Jun 21. Epub 2020 Jun 21.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education); SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Formate can be efficiently produced via electrochemical or photochemical catalytic conversion of CO, and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Consequently, the construction of efficient formate-assimilation pathways in microorganisms is essential for the utilization of cheap, renewable one-carbon compounds. Read More

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http://dx.doi.org/10.1007/s00253-020-10725-6DOI Listing

T cell immunity rather than antibody mediates cross-protection against Zika virus infection conferred by a live attenuated Japanese encephalitis SA14-14-2 vaccine.

Appl Microbiol Biotechnol 2020 Jun 15. Epub 2020 Jun 15.

Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.

Zika virus (ZIKV) and Japanese encephalitis virus (JEV) are closely related to mosquito-borne flaviviruses. Japanese encephalitis (JE) vaccine SA14-14-2 has been in the Chinese national Expanded Program on Immunization since 2007. The recent recognition of severe disease syndromes associated with ZIKV, and the identification of ZIKV from mosquitoes in China, prompts an urgent need to investigate the potential interaction between the two. Read More

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http://dx.doi.org/10.1007/s00253-020-10710-zDOI Listing

New insights into two yeast BDHs from the PDH subfamily as aldehyde reductases in context of detoxification of lignocellulosic aldehyde inhibitors.

Appl Microbiol Biotechnol 2020 Jun 15. Epub 2020 Jun 15.

Institute of Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.

At least 24 aldehyde reductases from Saccharomyces cerevisiae have been characterized and most function in in situ detoxification of lignocellulosic aldehyde inhibitors, but none is classified into the polyol dehydrogenase (PDH) subfamily of the medium-chain dehydrogenase/reductase (MDR) superfamily. This study confirmed that two (2R,3R)-2,3-butanediol dehydrogenases (BDHs) from industrial (denoted Y)/laboratory (denoted B) strains of S. cerevisiae, Bdh1p(Y)/Bdh1p(B) and Bdh2p(Y)/Bdh2p(B), were members of the PDH subfamily with an NAD(P)H binding domain and a catalytic zinc binding domain, and exhibited reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. Read More

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http://dx.doi.org/10.1007/s00253-020-10722-9DOI Listing

Anticancer potentiality and mode of action of low-carbohydrate proteins and peptides from mushrooms.

Appl Microbiol Biotechnol 2020 Jun 15. Epub 2020 Jun 15.

Recombinant Proteins Department, Breast Cancer Research Center, Moatamed Cancer Institute, ACECR, Tehran, Iran.

Severe side effects of chemotherapy as well as drug resistance highlight the ongoing need to discover novel natural bioactive compounds with anticancer potentiality. Mushroom-derived proteins are among the naturally occurring compounds that have been the subject of a body of research on their potentiality in cancer therapy. The greatest attention in relevant review articles has been paid to well-known mushroom-derived glycoproteins such as lectins and protein-bound polysaccharide complexes such as polysaccharide-K (PSK) or krestin and polysaccharopeptide (PSP), which contain substantial amounts of carbohydrates (50-90%). Read More

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http://dx.doi.org/10.1007/s00253-020-10707-8DOI Listing

An overview on marine cellulolytic enzymes and their potential applications.

Appl Microbiol Biotechnol 2020 Jun 17. Epub 2020 Jun 17.

Department of Microbiology, University of Karachi, Karachi, 75270, Pakistan.

Marine-derived enzymes have recently gained attention particularly for industrial applications. Cellulose-degrading enzymes are among leading biocatalysts with potential utility in biorefineries. This review presents an account of the cellulase production by marine sources from microorganisms including bacteria, yeasts, and molds to marine invertebrates such as protist, rotifer, mollusks, arthropods, and echinoderms. Read More

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http://dx.doi.org/10.1007/s00253-020-10692-yDOI Listing

Compound Lactobacillus sp. administration ameliorates stress and body growth through gut microbiota optimization on weaning piglets.

Appl Microbiol Biotechnol 2020 Jun 17. Epub 2020 Jun 17.

Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agriculture Science, Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, No. 40 of NongKe South of Road, Hefei, 230031, Anhui, China.

The composition of bacteria in the gastrointestinal tract of piglets is easily affected by environmental changes, particularly during the weaning period. Compound strains of Lactobacillus reuteri and Lactobacillus salivarius were supplemented to piglets during pre- and post-weaning to determine their effects in improving the growth performance and ameliorating the diarrhea rate and stress caused by antioxidation in piglets. A larger number of L. Read More

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http://dx.doi.org/10.1007/s00253-020-10727-4DOI Listing

Anaerobic glucose consumption is accelerated at non-proliferating elevated temperatures through upregulation of a glucose transporter gene in Corynebacterium glutamicum.

Appl Microbiol Biotechnol 2020 Jun 17. Epub 2020 Jun 17.

Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, Japan.

Cell proliferation is achieved through numerous enzyme reactions. Temperature governs the activity of each enzyme, ultimately determining the optimal growth temperature. The synthesis of useful chemicals and fuels utilizes a fraction of available metabolic pathways, primarily central metabolic pathways including glycolysis and the tricarboxylic acid cycle. Read More

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http://dx.doi.org/10.1007/s00253-020-10739-0DOI Listing

Design, application, and microbiome of sulfate-reducing bioreactors for treatment of mining-influenced water.

Appl Microbiol Biotechnol 2020 Jun 18. Epub 2020 Jun 18.

Japan Oil, Gas and Metals National Corporation (JOGMEC), 2-10-1 Toranomon, Minato-ku, Tokyo, 105-0001, Japan.

Sulfate-reducing bioreactors, also called biochemical reactors, represent a promising option for passive treatment of mining-influenced water (MIW) based on similar technology to aerobic/anaerobic-constructed wetlands and vertical-flow wetlands. MIW from each mine site has a variety of site-specific properties related to its treatment; therefore, design factors, including the organic substrates and inorganic materials packed into the bioreactor, must be tested and evaluated before installation of full-scale sulfate-reducing bioreactors. Several full-scale sulfate-reducing bioreactors operating at mine sites provide examples, but holistic understanding of the complex treatment processes occurring inside the bioreactors is lacking. Read More

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http://dx.doi.org/10.1007/s00253-020-10737-2DOI Listing

Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data.

Appl Microbiol Biotechnol 2020 Jun 17. Epub 2020 Jun 17.

College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.

Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. Read More

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http://dx.doi.org/10.1007/s00253-020-10715-8DOI Listing

Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity.

Appl Microbiol Biotechnol 2020 Jun 16. Epub 2020 Jun 16.

Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, MOA Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.

Bacterial magnetic particles (BMPs) are biosynthesized magnetic nano-scale materials with excellent dispersibility and biomembrane enclosure properties. In this study, we demonstrate that BMPs augment the ability of polyethylenimine (PEI) to deliver target DNA into difficult-to-transfect primary porcine liver cells, with transfection efficiency reaching over 30%. Compared with standard lipofection and polyfection, BMP-PEI gene vectors significantly enhanced the transfection efficiencies for the primary porcine liver cells and C2C12 mouse myoblast cell lines. Read More

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http://dx.doi.org/10.1007/s00253-020-10729-2DOI Listing

Subcellular localization of Sur7 and its pleiotropic effect on cell wall integrity, multiple stress responses, and virulence of Beauveria bassiana.

Appl Microbiol Biotechnol 2020 Jun 16. Epub 2020 Jun 16.

Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.

Sur7 is one of multiple proteins constituting MCC (membrane compartment of Can1 acting as an arginine/H symporter), a crucial membrane domain that can form punctuate eisosome spots on the plasma membrane and execute diverse functions in model yeast but remains poorly understood in filamentous fungi. Here, a Sur7 homolog bearing a typical SUR7 domain and four transmembrane domains was shown to localize in the conidial vesicles and enter vacuoles and appear sporadically on the periphery membrane during hyphal growth in the insect-pathogenic fungus Beauveria bassiana, implicating an involvement of Sur7 in cellular events linked to both plasma membrane and vacuoles. Deletion of sur7 resulted in reduced conidiation capacity and impaired conidial quality, which was featured by slower germination, attenuated virulence, and reduced carbohydrate epitopes (β-N-acetylglucosamine and sialic acids). Read More

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http://dx.doi.org/10.1007/s00253-020-10736-3DOI Listing

Several ways one goal-methanogenesis from unconventional substrates.

Appl Microbiol Biotechnol 2020 Jun 15. Epub 2020 Jun 15.

Department of Microbiology, Institute for Water and Wetland Research, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.

Methane is the second most important greenhouse gas on earth. It is produced by methanogenic archaea, which play an important role in the global carbon cycle. Three main methanogenesis pathways are known: in the hydrogenotrophic pathway H and carbon dioxide are used for methane production, whereas in the methylotrophic pathway small methylated carbon compounds like methanol and methylated amines are used. Read More

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http://dx.doi.org/10.1007/s00253-020-10724-7DOI Listing

Indian sarsaparilla, Hemidesmus indicus (L.) R. Br. ex Schult: tissue culture studies.

Appl Microbiol Biotechnol 2020 Jun 14. Epub 2020 Jun 14.

Independent researcher, Miki-cho Post Office, P. O. Box no. 7, Ikenobe, Kagawa-ken, 3011-2, 761-0799, Japan.

Hemidesmus indicus (L.) R. Br. Read More

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http://dx.doi.org/10.1007/s00253-020-10714-9DOI Listing

Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917.

Appl Microbiol Biotechnol 2020 Jun 13. Epub 2020 Jun 13.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.

Toxin-antitoxin systems (TASs) have attracted much attention due to their important physiological functions. These small genetic factors have been widely studied mostly in commensal Escherichia coli strains, whereas the role of TASs in the probiotic E. coli Nissle 1917 (EcN) is still elusive. Read More

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http://dx.doi.org/10.1007/s00253-020-10733-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293176PMC

Cyclo(Phe-Pro) produced by Vibrio species passes through biological membranes by simple diffusion.

Appl Microbiol Biotechnol 2020 Jun 13. Epub 2020 Jun 13.

Department of Life Science, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 04107, South Korea.

Cyclo(Phe-Pro) (cFP), produced by the Vibrio species, plays the dual roles of being a signaling molecule and a virulence factor. Acting modes of this compound have recently been characterized at the molecular level. Nevertheless, the method by which this compound passes across biological membranes remains obscure. Read More

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http://dx.doi.org/10.1007/s00253-020-10646-4DOI Listing

Lactic acid accumulation under heat stress related to accelerated glycolysis and mitochondrial dysfunction inhibits the mycelial growth of Pleurotus ostreatus.

Appl Microbiol Biotechnol 2020 Jun 12. Epub 2020 Jun 12.

Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

High temperature is a major threat to Pleurotus ostreatus cultivation. In this study, a potential mechanism by which P. ostreatus mycelia growth is inhibited under heat stress was explored. Read More

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http://dx.doi.org/10.1007/s00253-020-10718-5DOI Listing

Microorganisms populating the water-related indoor biome.

Appl Microbiol Biotechnol 2020 Jun 12. Epub 2020 Jun 12.

Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.

Modernisation of our households created novel opportunities for microbial growth and thus changed the array of microorganisms we come in contact with. While many studies have investigated microorganisms in the air and dust, tap water, another major input of microbial propagules, has received far less attention. The quality of drinking water in developed world is strictly regulated to prevent immediate danger to human health. Read More

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http://dx.doi.org/10.1007/s00253-020-10719-4DOI Listing

Ribose-5-phosphate isomerases: characteristics, structural features, and applications.

Appl Microbiol Biotechnol 2020 Jun 12. Epub 2020 Jun 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Ribose-5-phosphate isomerase (Rpi, EC 5.3.1. Read More

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http://dx.doi.org/10.1007/s00253-020-10735-4DOI Listing

Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6.

Appl Microbiol Biotechnol 2020 Jun 12. Epub 2020 Jun 12.

National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology; College of Life Sciences, Fujian Normal University, Fuzhou, 350117, Fujian, People's Republic of China.

Oxygen is a vital parameter for pyrroloquinoline quinone (PQQ) biosynthesis. In this study, the effects of oxygen supply on the biosynthesis of PQQ were first investigated systematically with Hyphomicrobium denitrificans FJNU-6. Following a kinetic analysis of the specific cell growth rate (μ) and specific PQQ formation rate (μ) in 5 L benchtop fermentation systems at various oxygen supply levels ranging from 0 to 60%, a novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity. Read More

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http://dx.doi.org/10.1007/s00253-020-10690-0DOI Listing

Towards control of cellulose biosynthesis by Komagataeibacter using systems-level and strain engineering strategies: current progress and perspectives.

Appl Microbiol Biotechnol 2020 Jun 11. Epub 2020 Jun 11.

Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, B. Stefanowskiego 4/10, 90-924, Lodz, Poland.

The strains of the Komagataeibacter genus have been shown to be the most efficient bacterial nanocellulose producers. Although exploited for many decades, the studies of these species focused mainly on the optimisation of cellulose synthesis process through modification of culturing conditions in the industrially relevant settings. Molecular physiology of Komagataeibacter was poorly understood and only a few studies explored genetic engineering as a strategy for strain improvement. Read More

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http://dx.doi.org/10.1007/s00253-020-10671-3DOI Listing

Thermostable arginase from Sulfobacillus acidophilus with neutral pH optimum applied for high-efficiency L-ornithine production.

Appl Microbiol Biotechnol 2020 Jun 11. Epub 2020 Jun 11.

School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai, People's Republic of China.

This study aims to use neutral pH optimum arginase as the catalyst for high-efficiency L-ornithine production. Sulfobacillus acidophilus arginase was firstly cloned and overexpressed in Escherichia coli. The purified enzyme was obtained, and the molecular mass determination showed that this arginase was a hexamer. Read More

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http://dx.doi.org/10.1007/s00253-020-10721-wDOI Listing

Correction to: Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.

Appl Microbiol Biotechnol 2020 Jul;104(14):6427

Department of Food Science, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil.

There is an error in the original publication of this paper for the below data was missed by author. It should be under "Conflict of interest" statement. Read More

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http://dx.doi.org/10.1007/s00253-020-10738-1DOI Listing

Effects of dietary rumen-protected Lys levels on rumen fermentation and bacterial community composition in Holstein heifers.

Appl Microbiol Biotechnol 2020 Jun 9. Epub 2020 Jun 9.

Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Sino-US Joint Lab on Nutrition and Metabolism of Ruminants, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

This study aimed to evaluate the effects of partial reducing rumen-protected Lys (RPLys) on rumen fermentation and microbial composition in heifers. Three ruminal fistulated Holstein Friesian bulls were used to determine the effective degradability of RPLys using an in situ method at incubation times of 0, 2, 6, 12, 16, 24, 36, and 48 h. Thereafter, 36 Holstein heifers at 90 days of age were assigned to one of two dietary treatments: a theoretically balanced amino acid diet (PC group; 1. Read More

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http://dx.doi.org/10.1007/s00253-020-10684-yDOI Listing

2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae.

Appl Microbiol Biotechnol 2020 Jun 9. Epub 2020 Jun 9.

Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China.

2,3-Dihydroxyisovalerate is an intermediate of valine and leucine biosynthesis pathway; however, no natural microorganism has been found yet that can accumulate this compound. Klebsiella pneumoniae is a useful bacterium that can be used as a workhorse for the production of a range of industrially desirable chemicals. Dihydroxy acid dehydratase, encoded by the ilvD gene, catalyzes the reaction of 2-ketoisovalerate formation from 2,3-dihydroxyisovalerate. Read More

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http://dx.doi.org/10.1007/s00253-020-10711-yDOI Listing
June 2020
3.337 Impact Factor

Effect of Rhizophora apiculata plantation for improving water quality, growth, and health of mud crab.

Appl Microbiol Biotechnol 2020 Jun 9. Epub 2020 Jun 9.

School of Marine Sciences, Ningbo University, Ningbo, 315211, China.

A deteriorated water quality is closely associated with disease outbreaks in aquaculture, where microorganisms play indispensable roles in improving water quality and aquatic animals' health. Mangrove is known to be a natural water quality filter and microbiological buffer of pathogen and prebiotics. However, it is unclear how and to what extent Rhizophora apiculata plantation is of benefits to the gut microbiota and growth over mud crab (Scylla paramamosain) aging. Read More

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http://dx.doi.org/10.1007/s00253-020-10716-7DOI Listing

Advances on the in vivo and in vitro glycosylations of flavonoids.

Appl Microbiol Biotechnol 2020 Jun 8. Epub 2020 Jun 8.

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, China.

Flavonoids possess diverse bioactivity and potential medicinal values. Glycosylation of flavonoids, coupling flavonoid aglycones and glycosyl groups in conjugated form, can change the biological activity of flavonoids, increase water solubility, reduce toxic and side effects, and improve specific targeting. Therefore, it is desirable to synthesize various flavonoid glycosides for further investigation on their medicinal values. Read More

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http://dx.doi.org/10.1007/s00253-020-10667-zDOI Listing

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice.

Appl Microbiol Biotechnol 2020 Jun 6. Epub 2020 Jun 6.

Team of Alternatives to Antibiotics, Gene Engineering Lab, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081, People's Republic of China.

Staphylococcus aureus is a common pathogen that can cause clinical and subclinical endometritis in humans and animals. In this study, a designed CSαβ peptide ID13 from DLP4 exhibited high stable antibacterial activity in simulated gastric fluid (90.79%), serum (99. Read More

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http://dx.doi.org/10.1007/s00253-020-10685-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275135PMC

Flavin-dependent N-hydroxylating enzymes: distribution and application.

Appl Microbiol Biotechnol 2020 Jun 5. Epub 2020 Jun 5.

Microbial Biotechnology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany.

Amino groups derived from naturally abundant amino acids or (di)amines can be used as "shuttles" in nature for oxygen transfer to provide intermediates or products comprising N-O functional groups such as N-hydroxy, oxazine, isoxazolidine, nitro, nitrone, oxime, C-, S-, or N-nitroso, and azoxy units. To this end, molecular oxygen is activated by flavin, heme, or metal cofactor-containing enzymes and transferred to initially obtain N-hydroxy compounds, which can be further functionalized. In this review, we focus on flavin-dependent N-hydroxylating enzymes, which play a major role in the production of secondary metabolites, such as siderophores or antimicrobial agents. Read More

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http://dx.doi.org/10.1007/s00253-020-10705-wDOI Listing

A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate.

Appl Microbiol Biotechnol 2020 Jun 4. Epub 2020 Jun 4.

Institute of Microbiology, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.

Polyphosphosphate kinases (PPKs) catalyse the reversible transfer of the γ-phosphate group of a nucleoside-triphosphate to a growing chain of polyphosphate. Most known PPKs are specific for ATP, but some can also use GTP as a phosphate donor. In this study, we describe the properties of a PPK2-type PPK of the β-proteobacterium Ralstonia eutropha. Read More

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http://dx.doi.org/10.1007/s00253-020-10706-9DOI Listing