12,407 results match your criteria Applied microbiology and biotechnology[Journal]


Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application.

Appl Microbiol Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, 11000, Serbia.

Application of agro-industrial by-products for the production of lactic acid was studied in this paper. Brewer's spent grain (BSG), malt rootlets (MR), brewer's yeast (BY), and soy lecithin (SL) were used as raw materials in L-(+)-LA fermentation by free and immobilized Lactobacillus rhamnosus ATCC 7469. The BSG, solid remains after BSG and MR hydrolysis (BSGMRSR), and MR were evaluated as carriers for batch and repeated batch fermentations with immobilized cells. Read More

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http://dx.doi.org/10.1007/s00253-019-09683-5DOI Listing
February 2019

Occurrence, function, and biosynthesis of mycofactocin.

Appl Microbiol Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Department of Chemistry and Biochemistry, University of Denver, Denver, CO, 80208, USA.

Mycofactocin is a member of the rapidly growing class of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Although the mycofactocin biosynthetic pathway is widely distributed among Mycobacterial species, the structure, function, and biosynthesis of the pathway product remain unknown. This mini-review will discuss the current state of knowledge regarding the mycofactocin biosynthetic pathway. Read More

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http://dx.doi.org/10.1007/s00253-019-09684-4DOI Listing
February 2019

Biological synthesis of metallic nanoparticles (MNPs) by plants and microbes: their cellular uptake, biocompatibility, and biomedical applications.

Appl Microbiol Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Key Laboratory for Space Bioscience & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, People's Republic of China.

Metallic nanoparticles (MNPs) with their diverse physical and chemical properties have been applied in various biomedical domains. The increasing demand for MNPs has attracted researchers to develop straightforward, inexpensive, simple, and eco-friendly processes for the enhanced production of MNPs. To discover new biomedical applications first requires knowledge of the interactions of MNPs with target cells. Read More

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http://dx.doi.org/10.1007/s00253-019-09675-5DOI Listing
February 2019

A high-risk papillomavirus 18 E7 affibody-enabled in vivo imaging and targeted therapy of cervical cancer.

Appl Microbiol Biotechnol 2019 Feb 15. Epub 2019 Feb 15.

Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, 325035, China.

High-risk papillomavirus (HPV) is one of the major reasons for cervical cancer, causing most lethal gynecologic malignancies worldwide. For cervical cancer progression, oncogene E7 plays vital roles and is used as one of the major targets for cervical tumor diagnosis and treatment. In the clinic, successful treatment of cervical cancer relies on diagnosing the disease at an early stage, where a late-stage diagnosis usually led to treatment failure. Read More

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http://link.springer.com/10.1007/s00253-019-09655-9
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http://dx.doi.org/10.1007/s00253-019-09655-9DOI Listing
February 2019
2 Reads

Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues.

Appl Microbiol Biotechnol 2019 Feb 15. Epub 2019 Feb 15.

CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Transcriptional regulatory proteins (TRPs)-based whole-cell biosensors are promising owing to their specificity and sensitivity, but their applications are currently limited. Herein, TRPs were adapted for the extracellular detection of a disease biomarker, uric acid, and a typical pesticide residue, carbaryl. A mutant regulatory protein that specifically recognizes carbaryl as its non-natural effector and activates transcription upon carbaryl binding was developed by engineering the regulatory protein TtgR from Pseudomonas putida. Read More

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http://dx.doi.org/10.1007/s00253-019-09679-1DOI Listing
February 2019

Reductive soil disinfestation effectively alleviates the replant failure of Sanqi ginseng through allelochemical degradation and pathogen suppression.

Appl Microbiol Biotechnol 2019 Feb 15. Epub 2019 Feb 15.

School of Geography Science, Nanjing Normal University, Nanjing, 210023, China.

Replant failure has threatened the production of Sanqi ginseng (Panax notoginseng) mainly due to the accumulation of soil-borne pathogens and allelochemicals. Reductive soil disinfestation (RSD) is an effective practice used to eliminate soil-borne pathogens; however, the potential impact of RSD on the degradation of allelochemicals and the growth of replant Sanqi ginseng seedlings remain poorly understood. In this study, RSD was conducted on a Sanqi ginseng monoculture system (SGMS) and a maize-Sanqi ginseng system (MSGS), defined as SGMS_RSD and MSGS_RSD, respectively. Read More

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http://dx.doi.org/10.1007/s00253-019-09676-4DOI Listing
February 2019

Expression and characterization of two glucuronoyl esterases from Thielavia terrestris and their application in enzymatic hydrolysis of corn bran.

Appl Microbiol Biotechnol 2019 Feb 14. Epub 2019 Feb 14.

The Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.

The thermophilic fungus Thielavia terrestris when cultured on cellulose produces a cocktail of thermal hydrolases with potential application in saccharification of lignocellulosic biomass and other biotechnological areas. Glucuronoyl esterases are considered to play a unique role as accessory enzymes in lignocellulosic material biodegradation by cleaving the covalent ester linkage between 4-O-methyl-D-glucuronic acid (MeGlcA) and lignin in lignin-carbohydrate complexes (LCCs). Two glucuronoyl esterases from T. Read More

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http://dx.doi.org/10.1007/s00253-019-09662-wDOI Listing
February 2019
1 Read

Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing.

Appl Microbiol Biotechnol 2019 Feb 14. Epub 2019 Feb 14.

Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.

Pseudomonas putida KT2440 is a Gram-negative, biosafety strain that plays important roles in environmental and biotechnological applications. Highly efficient genome editing strategy is essential to the elucidation of gene function and construction of metabolic engineered strains. Building on our previously established recombineering-mediated markerless and scarless P. Read More

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http://dx.doi.org/10.1007/s00253-019-09654-wDOI Listing
February 2019

Insights into the anti-infective properties of prodiginines.

Appl Microbiol Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

College of Biological, Chemical Sciences and Engineering, Jiaxing University, No. 118 Jiahang Road, Jiaxing, Zhejiang, 314001, People's Republic of China.

Prodiginines are a large family of tripyrrole alkaloids that contain natural members produced by various bacteria and non-natural members obtained from chemical synthesis, enzymatic synthesis, and mutasynthesis. These compounds have attracted a great deal of attention due to their wide range of fascinating properties including anti-infective, anticancer, and immunosuppressive activities. In consideration of the great need for novel and effective anti-infective agents, this review is mainly focused on the current status of research on the anti-infective properties of prodiginines, highlighting their antibacterial, antifungal, antiprotozoal, anti-larval, and antiviral activities. Read More

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http://link.springer.com/10.1007/s00253-019-09641-1
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http://dx.doi.org/10.1007/s00253-019-09641-1DOI Listing
February 2019
2 Reads

Citric acid and itaconic acid accumulation: variations of the same story?

Appl Microbiol Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

Institute of Chemical, Environmental & Bioscience Engineering, TU Wien, Getreidemarkt 9, 1060, Vienna, Austria.

Citric acid production by Aspergillus niger and itaconic acid production by Aspergillus terreus are two major examples of technical scale fungal fermentations based on metabolic overflow of primary metabolism. Both organic acids are formed by the same metabolic pathway, but whereas citric acid is the end product in A. niger, A. Read More

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http://dx.doi.org/10.1007/s00253-018-09607-9DOI Listing
February 2019

Synthesis of chitosan biocomposites loaded with pyrrole-2-carboxylic acid and assessment of their antifungal activity against Aspergillus niger.

Appl Microbiol Biotechnol 2019 Feb 12. Epub 2019 Feb 12.

Microbiology and Micotoxins Laboratory, Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, Mexico.

A wide variety of chitosan (CS) biomaterials have been loaded with different antimicrobial agents to improve the activity of CS against phytopathogenic fungi. Recently, the antimicrobial activity of 1H-pyrrole-2-carboxylic acid (PCA) has been reported as a secondary metabolite of Streptomyces griseus, which was identified as the main bioactive compound in the biological control. However, it is sensitive to light and its activity against filamentous fungi has not yet been reported. Read More

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http://dx.doi.org/10.1007/s00253-019-09670-wDOI Listing
February 2019

Identification and evaluation of novel anchoring proteins for cell surface display on Saccharomyces cerevisiae.

Appl Microbiol Biotechnol 2019 Feb 9. Epub 2019 Feb 9.

Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Luang, Pathumthani, 12120, Thailand.

The development of arming yeast strains as whole-cell biocatalysts involves a selection of effective anchoring proteins to display enzymes and proteins on yeast cell surface. To screen for novel anchoring proteins with improved efficiency, a bioinformatics pipeline for the identification of glycosylphosphatidylinositol-anchored cell wall proteins (GPI-CWPs) suitable for attaching passenger proteins to the cell surface of Saccharomyces cerevisiae has been developed. Here, the C-terminal sequences (CTSs) of putative GPI-CWPs were selected based on the criteria that the sequence must contain a serine/threonine-rich (S/T) region of at least 30% S/T content, a total threonine content of at least 10%, a continuous S/T stretch of at least 130 amino acids in length, and a continuous T-rich region of at least 10 amino acids in length. Read More

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http://dx.doi.org/10.1007/s00253-019-09667-5DOI Listing
February 2019
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Biocontrol of Brettanomyces/Dekkera bruxellensis in alcoholic fermentations using saccharomycin-overproducing Saccharomyces cerevisiae strains.

Appl Microbiol Biotechnol 2019 Feb 8. Epub 2019 Feb 8.

Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.

Microbial contamination of alcoholic fermentation processes (e.g. winemaking and fuel-ethanol production) is a serious problem for the industry since it may render the product unacceptable and/or reduce its productivity, leading to large economic losses. Read More

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http://dx.doi.org/10.1007/s00253-019-09657-7DOI Listing
February 2019
2 Reads

Enhanced activity and substrate tolerance of 7α-hydroxysteroid dehydrogenase by directed evolution for 7-ketolithocholic acid production.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Hunan Flag Bio-tech Co., Ltd., Changsha, 410100, China.

7-Ketolithocholic acid (7-KLCA) is an important intermediate for the synthesis of ursodeoxycholic acid (UDCA). UDCA is the main effective component of bear bile powder that is used in traditional Chinese medicine for the treatment of human cholesterol gallstones. 7α-Hydroxysteroid dehydrogenase (7α-HSDH) is the key enzyme used in the industrial production of 7-KLCA. Read More

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http://dx.doi.org/10.1007/s00253-019-09668-4DOI Listing
February 2019

Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA.

Lipogenesis is a complicated process involving global transcriptional reprogramming of lipogenic pathways. It is commonly believed that nitrogen starvation triggers a metabolic shift that reroutes carbon flux from Krebs cycles to lipogenesis. In this study, we systematically surveyed and dynamically profiled the transcriptional activity of 22 lipogenic promoters aiming to delineate a picture how nitrogen starvation regulates lipogenesis in Y. Read More

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http://dx.doi.org/10.1007/s00253-019-09664-8DOI Listing
February 2019
3 Reads

Identification of novel epitopes targeting non-structural protein 2 of PRRSV using monoclonal antibodies.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, 150069, Heilongjiang, China.

Porcine reproductive and respiratory syndrome virus (PRRSV) is leading to huge losses in the swine industry worldwide. Its nonstructural protein 2 (Nsp2), with a cysteine protease domain (PL2), is crucial for virus replication and as a trigger to host innate immune regulation. In this study, three monoclonal antibodies (mAbs) to Nsp2, designated 4A12, 4G8, and 8H11, were generated. Read More

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http://link.springer.com/10.1007/s00253-019-09665-7
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http://dx.doi.org/10.1007/s00253-019-09665-7DOI Listing
February 2019
2 Reads

GeM-Pro: a tool for genome functional mining and microbial profiling.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Laboratorio de Biotecnología e Inocuidad de los Alimentos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Municipalidad de Granadero Baigorria, Sede Suipacha 590, Rosario, Santa Fe, Argentina.

Gem-Pro is a new tool for gene mining and functional profiling of bacteria. It initially identifies homologous genes using BLAST and then applies three filtering steps to select orthologous gene pairs. The first one uses BLAST score values to identify trivial paralogs. Read More

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http://dx.doi.org/10.1007/s00253-019-09648-8DOI Listing
February 2019

Psychrophilic lifestyles: mechanisms of adaptation and biotechnological tools.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Institute of Microbiology, University of Innsbruck, 6020, Innsbruck, Austria.

Cold-adapted microorganisms inhabiting permanently low-temperature environments were initially just a biological curiosity but have emerged as rich sources of numerous valuable tools for application in a broad spectrum of innovative technologies. To overcome the multiple challenges inherent to life in their cold habitats, these microorganisms have developed a diverse array of highly sophisticated synergistic adaptations at all levels within their cells: from cell envelope and enzyme adaptation, to cryoprotectant and chaperone production, and novel metabolic capabilities. Basic research has provided valuable insights into how these microorganisms can thrive in their challenging habitat conditions and into the mechanisms of action of the various adaptive features employed, and such insights have served as a foundation for the knowledge-based development of numerous novel biotechnological tools. Read More

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http://dx.doi.org/10.1007/s00253-019-09659-5DOI Listing
February 2019

L-Cysteine production by metabolically engineered Corynebacterium glutamicum.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midoriku, Yokohama, Kanagawa, 226-8501, Japan.

L-Cysteine is a commercially important amino acid. Here, we report the construction of L-cysteine-producing Corynebacterium glutamicum using a metabolic engineering approach. L-Serine O-acetyltransferase (SAT), encoded by cysE gene, is a key enzyme of L-cysteine biosynthesis, because of its feedback inhibition by L-cysteine. Read More

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http://dx.doi.org/10.1007/s00253-019-09663-9DOI Listing
February 2019

Biodegradation of polyacrylic and polyester polyurethane coatings by enriched microbial communities.

Appl Microbiol Biotechnol 2019 Feb 6. Epub 2019 Feb 6.

Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ave. Universidad 3000. Col. UNAM, 04510, Mexico City, Mexico.

Microbial communities are more effective in degrading natural polymers and xenobiotics than pure cultures. Biodegradation of polyacrylic and polyurethane polymers by bacterial and fungal strains has been addressed, but limited information about their biodegradation by microbial communities exists. The aim of this work was to evaluate the ability of three enriched microbial communities (BP1h, BP3h, and BP7h), selected from deteriorated foam pieces collected in a landfill, to biodegrade the polyacrylic component of the 2K-PU coating Bayhydrol® A2470 and the polyester polyurethane coating NeoRez™ R-9637. Read More

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http://dx.doi.org/10.1007/s00253-019-09660-yDOI Listing
February 2019
2 Reads

Gene function and expression regulation of RuvRCAB in bacterial Cr(VI), As(III), Sb(III), and Cd(II) resistance.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Alishewanella sp. WH16-1 is a heavy metal-resistant bacterium. Previously, a putative YebC family regulator gene, designated ruvR, was associated with Cr(VI) resistance. Read More

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http://dx.doi.org/10.1007/s00253-019-09666-6DOI Listing
February 2019

Industrial uses of phospholipases: current state and future applications.

Appl Microbiol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), CONICET; y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Suipacha 531, 2000, Rosario, Argentina.

Phospholipids play a central role in all living organisms. Phospholipases, the enzymes aimed at modifying phospholipids, are consequently widespread in nature and play diverse roles, from lipid metabolism and cellular signaling in eukaryotes to virulence and nutrient acquisition in microbes. Phospholipases catalyze the hydrolysis of one or more ester or phosphodiester bonds of glycerophospholipids. Read More

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http://link.springer.com/10.1007/s00253-019-09658-6
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http://dx.doi.org/10.1007/s00253-019-09658-6DOI Listing
February 2019
1 Read

Endophyte-mediated synthesis of silver nanoparticles and their biological applications.

Appl Microbiol Biotechnol 2019 Feb 5. Epub 2019 Feb 5.

Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, 45000, Pakistan.

Biosynthesis has emerged as a frontier technology for fabrication of functionally diverse nanoparticles that possess tremendous therapeutic implications. Various biological resources have already demonstrated their potential to produce nanoparticles with interesting features. Endophytic microbes live in a symbiotic relationship with plants possessing a unique and versatile reservoir of potentially therapeutic secondary metabolites having the tendency to reduce metallic ions into nanoparticles. Read More

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http://dx.doi.org/10.1007/s00253-019-09661-xDOI Listing
February 2019
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Improving the production of isoprene and 1,3-propanediol by metabolically engineered Escherichia coli through recycling redox cofactor between the dual pathways.

Appl Microbiol Biotechnol 2019 Feb 5. Epub 2019 Feb 5.

CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Rd., Qingdao, 266101, China.

The biosynthesis of isoprene by microorganisms is a promising green route. However, the yield of isoprene is limited due to the generation of excess NAD(P)H via the mevalonate (MVA) pathway, which converts more glucose into CO or undesired reduced by-products. The production of 1,3-propanediol (1,3-PDO) from glycerol is a typical NAD(P)H-consuming process, which restricts 1,3-PDO yield to ~ 0. Read More

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http://dx.doi.org/10.1007/s00253-018-09578-xDOI Listing
February 2019

Microbial ecology of the cryosphere (glacial and permafrost habitats): current knowledge.

Appl Microbiol Biotechnol 2019 Feb 5. Epub 2019 Feb 5.

Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, 4710-057, Braga, Portugal.

Microorganisms in cold ecosystems play a key ecological role in their natural habitats. Since these ecosystems are especially sensitive to climate changes, as indicated by the worldwide retreat of glaciers and ice sheets as well as permafrost thawing, an understanding of the role and potential of microbial life in these habitats has become crucial. Emerging technologies have added significantly to our knowledge of abundance, functional activity, and lifestyles of microbial communities in cold environments. Read More

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http://dx.doi.org/10.1007/s00253-019-09631-3DOI Listing
February 2019

Introduction of heterologous transcription factors and their target genes into Penicillium oxalicum leads to increased lignocellulolytic enzyme production.

Appl Microbiol Biotechnol 2019 Feb 5. Epub 2019 Feb 5.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong Province, China.

Genetic engineering of transcription factors is an efficient strategy to improve lignocellulolytic enzyme production in fungi. In this study, the xylanase transcriptional regulators of Trichoderma reesei (Xyr1) and Neurospora crassa (XLR-1), as well as their constitutively active mutants (Xyr1 and XLR-1), were heterologously expressed in Penicillium oxalicum. The two heterologous regulators were identified to be able to activate lignocellulolytic enzyme gene expression in P. Read More

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http://dx.doi.org/10.1007/s00253-018-09612-yDOI Listing
February 2019

A genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris.

Appl Microbiol Biotechnol 2019 Feb 2. Epub 2019 Feb 2.

Department of Bioengineering, Gebze Technical University, Gebze, Kocaeli, Turkey.

Leuconostoc mesenteroides subsp. cremoris is an obligate heterolactic fermentative lactic acid bacterium that is mostly used in industrial dairy fermentations. The phosphoketolase pathway (PKP) is a unique feature of the obligate heterolactic fermentation, which leads to the production of lactate, ethanol, and/or acetate, and the final product profile of PKP highly depends on the energetics and redox state of the organism. Read More

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http://dx.doi.org/10.1007/s00253-019-09630-4DOI Listing
February 2019

Discovery and characterization of a novel C-terminal peptide carboxyl methyltransferase in a lassomycin-like lasso peptide biosynthetic pathway.

Appl Microbiol Biotechnol 2019 Feb 1. Epub 2019 Feb 1.

State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.

Lasso peptides belong to a peculiar family of ribosomally synthesized and post-translationally modified peptides (RiPPs)-natural products with an unusual isopeptide-bonded slipknot structure. Except for assembling of this unusual lasso fold, several further post-translational modifications of lasso peptides, including C-terminal methylation, phosphorylation/poly-phosphorylation, citrullination, and acetylation, have been reported recently. However, most of their biosynthetic logic have not been elucidated except the phosphorylated paeninodin lasso peptide. Read More

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http://dx.doi.org/10.1007/s00253-019-09645-xDOI Listing
February 2019
1 Read

Biotechnological production of glycolic acid and ethylene glycol: current state and perspectives.

Appl Microbiol Biotechnol 2019 Feb 1. Epub 2019 Feb 1.

Solutions for Natural Resources and Environment, VTT Technical Research Centre of Finland Ltd, Tietotie 2, P.O. Box 1000, 02044 VTT, Espoo, Finland.

Glycolic acid (GA) and ethylene glycol (EG) are versatile two-carbon organic chemicals used in multiple daily applications. GA and EG are currently produced by chemical synthesis, but their biotechnological production from renewable resources has received a substantial interest. Several different metabolic pathways by using genetically modified microorganisms, such as Escherichia coli, Corynebacterium glutamicum and yeast have been established for their production. Read More

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http://dx.doi.org/10.1007/s00253-019-09640-2DOI Listing
February 2019

Enhanced production and identification of antioxidants in in vitro cultures of the cacti Mammillaria candida and Turbinicarpus laui.

Appl Microbiol Biotechnol 2019 Feb 1. Epub 2019 Feb 1.

Facultad de Ciencias Químicas, UASLP, San Luis Potosí México, Manuel Nava 6, 78210, San Luis Potosí, S.L.P, Mexico.

Cacti are an important source of metabolites but present limitations for their commercial exploitation, like slow growth and a decrease of wild populations. An alternative to obtain their biocompounds without affecting the natural environment are the in vitro culture techniques. We established in vitro cultures from Mammillaria candida Scheidweiler and Turbinicarpus laui Glass and Foster and used different stresses to increase metabolites and antioxidant activity. Read More

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http://dx.doi.org/10.1007/s00253-019-09656-8DOI Listing
February 2019
1 Read

Antibacterial effects of iron oxide (FeO) nanoparticles: distinguishing concentration-dependent effects with different bacterial cells growth and membrane-associated mechanisms.

Appl Microbiol Biotechnol 2019 Feb 1. Epub 2019 Feb 1.

Department of Medical Biochemistry and Biotechnology, Russian-Armenian University, 123 H. Emin Str., 0051, Yerevan, Armenia.

Nowadays, the influence of nanoparticles (NPs) on microorganisms attracts a great deal of attention as an alternative to antibiotics. Iron oxide (FeO) NPs' effects on Gram-negative Escherichia coli BW 25113 and Gram-positive Enterococcus hirae ATCC 9790 growth and membrane-associated mechanisms have been investigated in this study. Growth specific rate of E. Read More

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http://dx.doi.org/10.1007/s00253-019-09653-xDOI Listing
February 2019
1 Read

Development of conditional cell lysis mutants of Saccharomyces cerevisiae as production hosts by modulating OCH1 and CHS3 expression.

Appl Microbiol Biotechnol 2019 Jan 31. Epub 2019 Jan 31.

Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, 06974, South Korea.

The traditional yeast Saccharomyces cerevisiae has been widely used as a host for the production of recombinant proteins and metabolites with industrial potential. However, its thick and rigid cell wall presents problems for the effective recovery of products. In this study, we modulated the expression of ScOCH1, encoding the α-1,6-mannosyltransferase responsible for outer chain biosynthesis of N-glycans, and ScCHS3, encoding the chitin synthase III required for synthesis of the majority of cell wall chitin, by exploiting the repressible ScMET3 promoter. Read More

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http://dx.doi.org/10.1007/s00253-019-09614-4DOI Listing
January 2019
1 Read

The production of ethanol from lignocellulosic biomass by Kluyveromyces marxianus CICC 1727-5 and Spathaspora passalidarum ATCC MYA-4345.

Appl Microbiol Biotechnol 2019 Jan 31. Epub 2019 Jan 31.

Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA.

Efficient bioconversion of lignocellulosic biomass is one of the key challenges for the production of bioethanol and chemicals. Therefore, the present work focuses on finding a robust microorganism able to convert all sugars in lignocellulosic hydrolysates efficiently. The fermentation performance showed that Kluyveromyces marxianus CICC 1727-5 could produce ethanol from glucose with productivity 4. Read More

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http://dx.doi.org/10.1007/s00253-019-09625-1DOI Listing
January 2019

Streptococcus macedonicus strains isolated from traditional fermented milks: resistance to gastrointestinal environment and adhesion ability.

Appl Microbiol Biotechnol 2019 Jan 30. Epub 2019 Jan 30.

CALBINOTOX, Université de Lorraine, F-54000, Nancy, France.

In this study, Streptococcus macedonicus (S. macedonicus) strains were identified from Algerian traditional fermented milks (Lben and Rayeb). Important prerequisites of probiotic interest such as acidity, bile salts tolerance, and adhesion ability to epithelial cells were investigated. Read More

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http://dx.doi.org/10.1007/s00253-019-09651-zDOI Listing
January 2019
1 Read

Forest gaps influence fungal community assembly in a weeping cypress forest.

Appl Microbiol Biotechnol 2019 Jan 29. Epub 2019 Jan 29.

Sichuan Province Key Laboratory of Ecological Security and Protection, Mianyang Normal University, Mianyang, 621000, China.

The forest gap crucially influences forest environments, but its effects on local fungal community assembly are not fully understood. In this study, the fungal community in a weeping cypress forest was investigated as a function of forest gap locations based on forest clearing, using amplicon sequencing of the ITS2 region. The results showed that the fungal community significantly varied with the variations in soil properties related to gap location. Read More

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http://link.springer.com/10.1007/s00253-018-09582-1
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http://dx.doi.org/10.1007/s00253-018-09582-1DOI Listing
January 2019
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Spotlight on fungal pectin utilization-from phytopathogenicity to molecular recognition and industrial applications.

Appl Microbiol Biotechnol 2019 Jan 29. Epub 2019 Jan 29.

Holzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising, Germany.

Pectin is a complex polysaccharide with D-galacturonic acid as its main component that predominantly accumulates in the middle lamella of the plant cell wall. Integrity and depolymerization of pectic structures have long been identified as relevant factors in fungal phytosymbiosis and phytopathogenicity in the context of tissue penetration and carbon source supply. While the pectic content of a plant cell wall can vary significantly, pectin was reported to account for up to 20-25% of the total dry weight in soft and non-woody tissues with non- or mildly lignified secondary cell walls, such as found in citrus peel, sugar beet pulp, and apple pomace. Read More

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http://dx.doi.org/10.1007/s00253-019-09622-4DOI Listing
January 2019
1 Read

Algal sorbent derived from Sargassum horneri for adsorption of cesium and strontium ions: equilibrium, kinetics, and mass transfer.

Appl Microbiol Biotechnol 2019 Jan 28. Epub 2019 Jan 28.

Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.

An algal sorbent derived from Sargassum horneri was prepared and used to adsorb cesium and strontium ions from aqueous solution. The phenomenological mathematical models associated to the predicted equilibrium isotherms were developed to determine the rate-limiting steps of the adsorption process. The maximum adsorption capacity of cesium ion and strontium ion was calculated to be 0. Read More

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http://dx.doi.org/10.1007/s00253-019-09619-zDOI Listing
January 2019

Insertions of antihypertensive peptides and their applications in pharmacy and functional foods.

Appl Microbiol Biotechnol 2019 Jan 28. Epub 2019 Jan 28.

Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, CIBA-IPN, Tepetitla, 90700, Tlaxcala, Mexico.

Hypertension is a worldwide health problem. It is the main cardiovascular risk factor and affects about 31% of the world's adult population. The drugs used to control hypertension may cause side effects; for this reason, there are many investigations focused on searching for alternatives to control or prevent this disease through diet. Read More

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http://dx.doi.org/10.1007/s00253-019-09633-1DOI Listing
January 2019
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Development of biodegradable coatings for maize seeds and their application for Azospirillum brasilense immobilization.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO BOX 6001, Londrina, PR, 86051-990, Brazil.

The objective of this study was to develop biodegradable coatings for agriculture crop seeds based on starch, gelatin, and polyvinyl alcohol (PVA). Developed materials were characterized according to their microstructures, barrier properties, influence on germination of maize seeds, and ability to sustain Azospirillum brasilense Ab-V5 viability in coated maize seeds. The coatings were obtained employing different proportions of starch, gelatin, and PVA, ranging from 0 to 3. Read More

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http://dx.doi.org/10.1007/s00253-019-09646-wDOI Listing
January 2019
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Comparative analysis of two non-specific nucleases of the phospholipase D family from the plant pathogen competitor bacterium Pantoea agglomerans.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, 51429, Bergisch Gladbach, Germany.

Bacterial non-specific nucleases of the phospholipase D family are widely distributed among the members of the Enterobacteriaceae. Each genome mainly contains a single copy of a gene encoding a phospholipase D family protein. However, two distantly related isozymes (< 40% identity at the protein level) were identified by BLAST-analyses in the plant pathogenic competitor enterobacterium Pantoea agglomerans. Read More

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http://dx.doi.org/10.1007/s00253-019-09644-yDOI Listing
January 2019
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Bovine colostrum-driven modulation of intestinal epithelial cells for increased commensal colonisation.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Teagasc Food Research Centre, Moorepark, Fermoy Co., Cork, P61C996, Ireland.

Nutritional intake may influence the intestinal epithelial glycome and in turn the available attachment sites for bacteria. In this study, we tested the hypothesis that bovine colostrum may influence the intestinal cell surface and in turn the attachment of commensal organisms. Human HT-29 intestinal cells were exposed to a bovine colostrum fraction (BCF) rich in free oligosaccharides. Read More

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http://dx.doi.org/10.1007/s00253-019-09642-0DOI Listing
January 2019
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Immobilization of cellulase in the non-natural ionic liquid environments to enhance cellulase activity and functional stability.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou, 215009, People's Republic of China.

Ionic liquids (ILs) have been applied as an environmentally friendly solvent in the pretreatment of lignocellulosic biomass for more than a decade. The ILs involved pretreatment processes for cellulases mediated saccharification lead to both the breakdown of cellulose crystallinity and the decrease of lignin content, thereby improving the solubility of cellulose and the accessibility of cellulase. However, most cellulases are partially or completely inactivated in the presence of even low amount of ILs. Read More

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http://dx.doi.org/10.1007/s00253-019-09647-9DOI Listing
January 2019
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Agronomic and environmental factors affecting cultivation of the winter mushroom or Enokitake: achievements and prospects.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Industrial Fungi Biotechnology Research Department, Research Institute for Industrial Biotechnology, Academic Center for Education, Culture and Research (ACECR) - Khorasan Razavi Branch, P.O. Box: 91775-1376, Mashhad, Iran.

The global interest in production of the winter mushroom or Enokitake (Flammulina filiformis previously known as Flammulina velutipes) is increasing owing to its nutritional and medicinally important bioactive compounds along with a marketable texture and flavor. This review presents the state of knowledge on achievements in solid-state cultivation and submerged cultures of Enokitake and how they are influenced by environmental factors and agronomic characteristics. A wide range of basic lignocellulosic substrates and supplementations have been reviewed in order to formulate an efficient and locally available substrate. Read More

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http://dx.doi.org/10.1007/s00253-019-09652-yDOI Listing
January 2019
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Evaluation and optimization of a methodology for the long-term cryogenic storage of Tetradesmus obliquus at - 80 °C.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Natural Products Biotechnology Group, Centro de Investigación en Agrosistemas Intensivos Mediterráneos y Biotecnología Agroalimentaria (CIAMBITAL), and Campus de Excelencia Internacional del Mar (CEI-Mar), University of Almeria, 04120, Almería, Spain.

Cryopreservation is a common methodology for long-term microalgae storage. Current cryopreservation methods are based on using diverse cryoprotectants and two-step cooling protocols, followed by sample storage at the temperature of liquid nitrogen (- 196 °C). However, the use of this methodology requires a continuous liquid N supply as well as facilities with dedicated equipment, which is not affordable for every laboratory. Read More

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http://dx.doi.org/10.1007/s00253-019-09650-0DOI Listing
January 2019
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The repertoire of effector candidates in Colletotrichum lindemuthianum reveals important information about Colletotrichum genus lifestyle.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

Laboratório de Genética Molecular de Fungos (LGMF)/BIOAGRO, Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, CEP: 36570-900, Brazil.

The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean (Phaseolus vulgaris), and anthracnose is one of the most devastating diseases of this plant species. However, little is known about the proteins that are essential for the fungus-plant interactions. Knowledge of the fungus' arsenal of effector proteins is of great importance for understanding this pathosystem. Read More

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http://dx.doi.org/10.1007/s00253-019-09639-9DOI Listing
January 2019
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Enhancement of neomycin production by engineering the entire biosynthetic gene cluster and feeding key precursors in Streptomyces fradiae CGMCC 4.576.

Appl Microbiol Biotechnol 2019 Jan 26. Epub 2019 Jan 26.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Neomycin, an aminoglycoside antibiotic, is widely used in the livestock husbandry due to its higher antimicrobial activity and availability of feed additives in animals. However, its production yield is relatively low and cannot meet the needs of developing market and clinical application. Here, the entire natural neo cluster was cloned from Streptomyces fradiae CGMCC 4. Read More

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http://dx.doi.org/10.1007/s00253-018-09597-8DOI Listing
January 2019
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Research progress and application prospect of anaerobic biological phosphorus removal.

Appl Microbiol Biotechnol 2019 Jan 25. Epub 2019 Jan 25.

Guangzhou University, Guangzhou, 510006, Guangdong, China.

Anaerobic biological phosphorus removal has proposed a new direction for the removal of phosphorus from wastewater, and the discovery of phosphate reduction makes people have a more comprehensive understanding of microbial phosphorus cycling. Here, from the perspective of thermodynamics, the bioreduction reaction of phosphate was analyzed and its mechanism was discussed. The research progress of phosphate reduction and the application prospects of anaerobic biological phosphorus removal from wastewater were introduced, pointing out the situation and guiding the further research in this field. Read More

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http://dx.doi.org/10.1007/s00253-019-09634-0DOI Listing
January 2019
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Trichosporon fermentans biomass flocculation from soybean oil refinery wastewater using bioflocculant produced from Paecilomyces sp. M2-1.

Appl Microbiol Biotechnol 2019 Jan 24. Epub 2019 Jan 24.

Sci-Tech Center for Clean Conversion and High-valued Utilization of Biomass, Jilin Province, Northeast Electric Power University, Jilin, 132012, China.

The soybean oil refinery (SOR) wastewater contains a high concentration of chemical oxygen demand (COD) and lipid, so the direct emissions of SOR wastewater will result in environmental pollution and waste of resources. Oleaginous yeast Trichosporon fermentans can consume organic materials in SOR wastewater to synthesize microbial oil, which achieves the purpose of SOR wastewater resource utilization. The effective harvesting technology of oleaginous yeasts can improve the utilization efficiency. Read More

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http://dx.doi.org/10.1007/s00253-019-09643-zDOI Listing
January 2019
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Bacteriophage-host arm race: an update on the mechanism of phage resistance in bacteria and revenge of the phage with the perspective for phage therapy.

Appl Microbiol Biotechnol 2019 Jan 24. Epub 2019 Jan 24.

School of Life Science and Technology, Tokyo Institute of Technology, 4259 J2-15 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.

Due to a constant attack by phage, bacteria in the environment have evolved diverse mechanisms to defend themselves. Several reviews on phage resistance mechanisms have been published elsewhere. Thanks to the advancement of molecular techniques, several new phage resistance mechanisms were recently identified. Read More

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http://dx.doi.org/10.1007/s00253-019-09629-xDOI Listing
January 2019

Engineering ClpS for selective and enhanced N-terminal amino acid binding.

Appl Microbiol Biotechnol 2019 Jan 24. Epub 2019 Jan 24.

Institute for Bioscience and Biotechnology Research (IBBR), National Institute of Standards and Technology (NIST) and the University of Maryland (UMD), 9600 Gudelsky Drive, Rockville, MD, 20850, USA.

One of the central challenges in the development of single-molecule protein sequencing technologies is achieving high-fidelity sequential recognition and detection of specific amino acids that comprise the peptide sequence. An approach towards achieving this goal is to leverage naturally occurring proteins that function through recognition of amino (N)-terminal amino acids (NAAs). One such protein, the N-end rule pathway adaptor protein ClpS, natively recognizes NAAs on a peptide chain. Read More

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http://dx.doi.org/10.1007/s00253-019-09624-2DOI Listing
January 2019
5 Reads