12,532 results match your criteria Applied microbiology and biotechnology[Journal]


Increasing the homologous recombination efficiency of eukaryotic microorganisms for enhanced genome engineering.

Appl Microbiol Biotechnol 2019 Apr 24. Epub 2019 Apr 24.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.

In recent years, eukaryotic microorganisms have been widely applied to offer many solutions for everyday life and have come to play important roles in agriculture, food, health care, and the fine-chemicals industry. However, the complex genetic background and low homologous recombination efficiency have hampered the implementation of large-scale and high-throughput gene editing in many eukaryotic microorganisms. The low efficiency of homologous recombination (HR) not only makes the modification process labor-intensive but also completely precludes the application of many otherwise very useful genome editing techniques. Read More

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http://dx.doi.org/10.1007/s00253-019-09802-2DOI Listing

Co-occurrence of functional modules derived from nicotine-degrading gene clusters confers additive effects in Pseudomonas sp. JY-Q.

Appl Microbiol Biotechnol 2019 Apr 23. Epub 2019 Apr 23.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.

Pseudomonas sp. JY-Q was isolated from nicotine-rich environment and could degrade and tolerate high-content nicotine. Its specific genetic architecture comprised duplicated homologous nicotine-degrading clusters for different functional modules on the whole pathway. Read More

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http://dx.doi.org/10.1007/s00253-019-09800-4DOI Listing

Metagenome to phenome approach enables isolation and genomics characterization of Kalamiella piersonii gen. nov., sp. nov. from the International Space Station.

Appl Microbiol Biotechnol 2019 Apr 23. Epub 2019 Apr 23.

Jet Propulsion Laboratory, Biotechnology and Planetary Protection Group, M/S 89-2, California Institute of Technology, 4800 Oak Grove Dr, Pasadena, CA, 91109, USA.

Several evolutionarily distinct, near full-length draft metagenome-resolved genomes (MRG), were assembled from sequences recovered from the International Space Station (ISS) environments. The retrieval of MRGs facilitated the exploration of a large collection of archived strains (~ 500 isolates) and assisted in isolating seven related strains. The whole genome sequences (WGS) of seven ISS strains exhibited 100% identity to the 4. Read More

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http://dx.doi.org/10.1007/s00253-019-09813-zDOI Listing

Surfactin effectively inhibits Staphylococcus aureus adhesion and biofilm formation on surfaces.

Appl Microbiol Biotechnol 2019 Apr 22. Epub 2019 Apr 22.

College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Biosurfactants are amphiphilic compounds that composed of hydrophilic and hydrophobic moieties, which possess the ability of self-organizing between phases, reducing the interfacial tension, and forming aggregates such as micelles. This spontaneous process results in significant changes in surface properties that directly influence the adherence of microorganisms. In this study, the ability of surfactin, a biosurfactant produced by Bacillus subtilis in reducing adhesion and disrupting the presence of biofilm of Staphylococcus aureus (S. Read More

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http://link.springer.com/10.1007/s00253-019-09808-w
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http://dx.doi.org/10.1007/s00253-019-09808-wDOI Listing
April 2019
1 Read

The impacts of Schizosaccharomyces on winemaking.

Authors:
Santiago Benito

Appl Microbiol Biotechnol 2019 Apr 19. Epub 2019 Apr 19.

Department of Chemistry and Food Technology, Polytechnic University of Madrid, University Town, S/N, 28040, Madrid, Spain.

In the past century, yeasts from the genus Saccharomyces represented the only option in fermentation industries, such as winemaking, to produce wine, beer, and other fermented products. However, other genera are currently emerging to solve challenges in modern enology. Schizosaccharomyces pombe is showing promising results in solving specific challenges in northern, cool viticulture regions with highly acidic wines by deacidifying these wines through its malic acid metabolism. Read More

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http://link.springer.com/10.1007/s00253-019-09827-7
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http://dx.doi.org/10.1007/s00253-019-09827-7DOI Listing
April 2019
1 Read

Screening and evaluation of adventitious root lines of Panax notoginseng by morphology, gene expression, and metabolite profiles.

Appl Microbiol Biotechnol 2019 Apr 19. Epub 2019 Apr 19.

Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China.

Panax notoginseng is a commonly used Traditional Chinese Medicine (TCM) and has diverse pharmacological activities with triterpenoid saponins as its main active ingredient. In the cultivation of P. notoginseng, continuous cropping is a serious problem, which could induce reduced productivity, low tuber quality, and plant mortality. Read More

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http://dx.doi.org/10.1007/s00253-019-09778-zDOI Listing

Comparative global metabolite profiling of xylose-fermenting Saccharomyces cerevisiae SR8 and Scheffersomyces stipitis.

Appl Microbiol Biotechnol 2019 Apr 19. Epub 2019 Apr 19.

School of Food Science and Biotechnology, Kyungpook National University, Daegu, Korea.

Bioconversion of lignocellulosic biomass into ethanol requires efficient xylose fermentation. Previously, we developed an engineered Saccharomyces cerevisiae strain, named SR8, through rational and inverse metabolic engineering strategies, thereby improving its xylose fermentation and ethanol production. However, its fermentation characteristics have not yet been fully evaluated. Read More

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http://link.springer.com/10.1007/s00253-019-09829-5
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http://dx.doi.org/10.1007/s00253-019-09829-5DOI Listing
April 2019
1 Read

Genome- and MS-based mining of antibacterial chlorinated chromones and xanthones from the phytopathogenic fungus Bipolaris sorokiniana strain 11134.

Appl Microbiol Biotechnol 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Halogen substituents are important for biological activity in many compounds. Genome-based mining of halogenase along with its biosynthetic gene cluster provided an efficient approach for the discovery of naturally occurring organohalogen compounds. Analysis of the genome sequence of a phytopathogenic fungus Bipolaris sorokiniana 11134 revealed a polyketide gene cluster adjacent to a flavin-dependent halogenase capable of encoding halogenated polyketides, which are rarely reported in phytopathogenic fungi. Read More

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http://dx.doi.org/10.1007/s00253-019-09821-zDOI Listing

A novel multiplex xMAP assay for generic detection of avian, fish, and ruminant DNA in feed and feedstuffs.

Appl Microbiol Biotechnol 2019 Apr 19. Epub 2019 Apr 19.

Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, 510623, China.

The identification of animal species in feed and feedstuffs is important for detecting contamination and fraudulent replacement of animal components that might cause health and economic problems. A novel multiplex assay, based on xMAP technology and the generic detection of closely related species, was developed for the simultaneous differential detection of avian, fish, and ruminant DNA in products. Universal primers and probes specific to avian, fish, or ruminant species were designed to target a conserved mitochondrial DNA sequence in the 12S ribosomal RNA gene (rRNA). Read More

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http://link.springer.com/10.1007/s00253-019-09833-9
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http://dx.doi.org/10.1007/s00253-019-09833-9DOI Listing
April 2019
1 Read

Effect of different phosphorus concentrations on biodiesel production from Isochrysis zhangjiangensis under nitrogen sufficiency or deprivation condition.

Appl Microbiol Biotechnol 2019 Apr 18. Epub 2019 Apr 18.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

The effects of two nitrogen (N) concentrations combining with three phosphorus (P) concentrations on Isochrysis zhangjiangensis growth and formation of fatty acid (FA) were investigated in this study. Biomass concentration, mass fraction, and productivity of FA in I. zhangjiangensis were low in N-deprived media. Read More

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http://dx.doi.org/10.1007/s00253-019-09814-yDOI Listing

L-Erythrulose production with a multideletion strain of Gluconobacter oxydans.

Appl Microbiol Biotechnol 2019 Apr 18. Epub 2019 Apr 18.

Institute of Biochemical Engineering, Technical University of Munich, Boltzmannstr. 15, D-85748, Garching, Germany.

Many ketoses or organic acids can be produced by membrane-associated oxidation with Gluconobacter oxydans. In this study, the oxidation of meso-erythritol to L-erythrulose was investigated with the strain G. oxydans 621HΔupp BP. Read More

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http://link.springer.com/10.1007/s00253-019-09824-w
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http://dx.doi.org/10.1007/s00253-019-09824-wDOI Listing
April 2019
1 Read

Deletion of regulator-encoding genes fadR, fabR and iclR to increase L-threonine production in Escherichia coli.

Appl Microbiol Biotechnol 2019 Apr 18. Epub 2019 Apr 18.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Previously, we have developed an L-threonine-producing Escherichia coli strain TWF006 in which the regulator-encoding gene iclR was deleted. In this study, further modifications were performed on TWF006 to increase L-threonine yield. Firstly, the regulator-encoding gene fadR was deleted in TWF006, and the resulting strain TWF031 produced 18. Read More

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http://dx.doi.org/10.1007/s00253-019-09818-8DOI Listing

Capability of iturin from Bacillus subtilis to inhibit Candida albicans in vitro and in vivo.

Appl Microbiol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, 710072, Shaanxi Province, China.

Candida albicans is a fungal pathogen that is difficult to cure clinically. The current clinic C. albicans-inhibiting drugs are very harmful to humans. Read More

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http://link.springer.com/10.1007/s00253-019-09805-z
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http://dx.doi.org/10.1007/s00253-019-09805-zDOI Listing
April 2019
1 Read

Two amino acids missing of MtrA resulted in increased erythromycin level and altered phenotypes in Saccharopolyspora erythraea.

Appl Microbiol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

The MtrA-MtrB two-component regulatory system is highly conserved in Actinobacteria and plays crucial roles in cell cycle progression, cell morphology, antibiotic resistance, and osmoprotection. Previously, we revealed that the MtrA protein of Saccharopolyspora erythraea E3 strain (a high erythromycin-producing strain) had a two amino acid (H197 and V198) deletion in the DNA recognition helices of the C-terminal domain compared to the wild type S. erythraea strain NRRL2338. Read More

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http://dx.doi.org/10.1007/s00253-019-09825-9DOI Listing

Size and coating of engineered silver nanoparticles determine their ability to growth-independently inhibit aflatoxin biosynthesis in Aspergillus parasiticus.

Appl Microbiol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Department of Environmental Health Sciences, University of South Carolina, Columbia, SC, USA.

Recent studies from our laboratory indicate that engineered silver nanoparticles can inhibit aflatoxin biosynthesis even at concentrations at which they do not demonstrate antifungal activities on the aflatoxin-producing fungus. Whether such inhibition can be modified by altering the nanoparticles' physical properties remains unclear. In this study, we demonstrate that three differently sized citrated-coated silver nanoparticles denoted here as NP1, NP2, and NP3 (where, sizes of NP1 < NP2 < NP3) inhibit aflatoxin biosynthesis at different effective doses in Aspergillus parasiticus, the plant pathogenic filamentous fungus. Read More

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http://dx.doi.org/10.1007/s00253-019-09693-3DOI Listing

Peculiarities of Staphylococcus aureus phages and their possible application in phage therapy.

Appl Microbiol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

School of Life Science and Technology, Tokyo Institute of Technology, 4259 J2-15, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.

Bacteriophage has become an attractive alternative for the treatment of antibiotic-resistant Staphylococcus aureus. For the success of phage therapy, phage host range is an important criterion when considering a candidate phage. Most reviews of S. Read More

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http://dx.doi.org/10.1007/s00253-019-09810-2DOI Listing

Structural modulation of gut microbiota reveals Coix seed contributes to weight loss in mice.

Appl Microbiol Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

BGI Education Center, University of Chinese Academy of Sciences, Main building, Beishan Industrial Zone, Yantian District, Shenzhen, 518083, China.

Coix seed (CS) is widely used as food material and herbal medicine in Asian countries with hypolipidemic and anti-inflammatory properties. But whether CS takes effect by modulating the composition of the gut microbiota remains unknown. Here, three groups of mice were fed different diets for 5 weeks: standard chow, high fat (HF), and CS added to HF. Read More

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http://link.springer.com/10.1007/s00253-019-09786-z
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http://dx.doi.org/10.1007/s00253-019-09786-zDOI Listing
April 2019
2 Reads

Nanomaterials for the control of bacterial blight disease in pomegranate: quo vadis?

Appl Microbiol Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

ICAR-National Research Centre on Pomegranate, Kegaon, Solapur, Maharashtra, 413255, India.

Bacterial blight, caused by Xanthomonas axonopodis pv. punicae, Xap is a serious threat to commercially successful pomegranate (Punica granatum L) crop. Owing to the non-availability of disease-resistant varieties of pomegranate, integrated disease management involving change of season, adequate nutrition, and preventive sprays of bactericides is used to control Xap. Read More

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http://link.springer.com/10.1007/s00253-019-09740-z
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http://dx.doi.org/10.1007/s00253-019-09740-zDOI Listing
April 2019
1 Read

Xanthobacter-dominated biofilm as a novel source for high-value rhamnose.

Appl Microbiol Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

Laboratory of Renewable Resources Engineering, Purdue University, Potter Engineering Center, 500 Central Drive Room 216, West Lafayette, IN, 47907, USA.

Rhamnose is a high-value carbohydrate used in flavorings, aromatics, and pharmaceuticals. Current demand for rhamnose is filled through plant-based sources; however, microbially originated rhamnolipids have been proposed as an alternative source. A mixed microbial biofilm, cultured from a wastewater sludge, was found to comprise > 8 dry weight% rhamnose when provided volatile fatty acids as carbon source, and 24 dry weight% when given glucose. Read More

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http://link.springer.com/10.1007/s00253-019-09765-4
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http://dx.doi.org/10.1007/s00253-019-09765-4DOI Listing
April 2019
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Interaction of carbohydrate-binding modules with poly(ethylene terephthalate).

Appl Microbiol Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52425, Jülich, Germany.

Poly(ethylene terephthalate) (PET) is one of the most widely applied synthetic polymers, but its hydrophobicity is challenging for many industrial applications. Biotechnological modification of PET surface can be achieved by PET hydrolyzing cutinases. In order to increase the adsorption towards their unnatural substrate, the enzymes are fused to carbohydrate-binding modules (CBMs) leading to enhanced activity. Read More

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http://link.springer.com/10.1007/s00253-019-09760-9
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http://dx.doi.org/10.1007/s00253-019-09760-9DOI Listing
April 2019
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Engineered biosynthesis of cyclic lipopeptide locillomycins in surrogate host Bacillus velezensis FZB42 and derivative strains enhance antibacterial activity.

Appl Microbiol Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Locillomycins are cyclic lipononapeptides assembled by a nonlinear hexamodular NRPS and have strong antibacterial activity. In this study, we genetically engineered Bacillus velezensis FZB42 as a surrogate host for the heterologous expression of the loc gene cluster for locillomycins. The fosmid N13 containing whole loc gene cluster was screened from the B. Read More

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http://dx.doi.org/10.1007/s00253-019-09784-1DOI Listing

Effect of contamination with Lactobacillus fermentum I2 on ethanol production by Spathaspora passalidarum.

Appl Microbiol Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Chemical Engineering and Food Engineering Department, Santa Catarina Federal University, CP 476, Florianópolis, SC, Zip Code 88040-900, Brazil.

Second-generation bioethanol is a promising source of renewable energy. In Brazilian mills, the production of ethanol from sugarcane (first generation, 1G) is a consolidated process performed by Saccharomyces cerevisiae and characterized by high substrate concentrations, high cell density, and cell recycle. The main bacterial contaminants in 1G fermentation tanks are lactic acid bacteria, especially bacteria from the Lactobacillus genus, which is associated with a decrease in ethanol yield and yeast cell viability, among other negative effects. Read More

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http://dx.doi.org/10.1007/s00253-019-09779-yDOI Listing

Purification-independent immunoreagents obtained by displaying nanobodies on bacteria surface.

Appl Microbiol Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Environmental and Life Sciences, University of Nova Gorica, Vipavska cesta 13, SI-5000, Rožna Dolina, Nova Gorica, Slovenia.

The availability of preimmune libraries of antibody fragments allows for the fast generation of binders which can be expressed in both eukaryotic and prokaryotic systems. We exploited the recombinant nature of antibody fragments to demonstrate the possibility of expressing them as functional proteins displayed on the surface of Escherichia coli and by such a way to generate living reagents ready-to-use for diagnostics. Such immunoreagents were effectively exploited without the necessity of any purification step to prepare immunocapture surfaces suitable for the diagnostic of both cancer cells and toxic microalgae. Read More

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http://dx.doi.org/10.1007/s00253-019-09823-xDOI Listing

A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei.

Appl Microbiol Biotechnol 2019 Apr 13. Epub 2019 Apr 13.

State Key Laboratory of Microbial Technology, Shandong University, No.72 Binhai Road, Qingdao, 266237, People's Republic of China.

XYR1 is the key transcription activator for cellulase gene expression in the model filamentous fungus Trichoderma reesei, which is widely applied in the industry due to its excellent capability of secreting a large quantity of cellulases. Despite the essential role of XYR1, the regulation of its expression in T. reesei cellulolytic response is poorly understood. Read More

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http://dx.doi.org/10.1007/s00253-019-09739-6DOI Listing
April 2019
3 Reads

The role of ammonium oxidising bacteria (AOB) in ionic liquid 1-dodecylpyridinium chloride removal.

Appl Microbiol Biotechnol 2019 Apr 11. Epub 2019 Apr 11.

School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Ave, Singapore, 639798, Singapore.

Ionic liquids (IL) have emerged as the next-generation "green" solvent that can replace traditional organic solvent due to properties such as high thermal stability and no vapour pressure. However, their increased usage inevitably allows them to find their way into the environment. The objective of this study was to evaluate the role of autotrophic ammonia-oxidising bacteria (AOB) in the potential removal of 1-dodecylpyridinium chloride ([DPy]Cl) in both short- and long-term studies. Read More

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http://dx.doi.org/10.1007/s00253-019-09799-8DOI Listing

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum.

Appl Microbiol Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.

Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was required, an indication that these are fastidious microorganisms. Elimination of complex components and individual vitamins from the medium revealed that the only organic compounds required for growth were pantothenate, biotin and thiamine. Read More

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http://dx.doi.org/10.1007/s00253-019-09763-6DOI Listing

Quantification of outer membrane vesicles: a potential tool to compare response in Pseudomonas putida KT2440 to stress caused by alkanols.

Appl Microbiol Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany.

The bacterial release of outer membrane vesicles (OMVs) is an important physiological mechanism of Gram-negative bacteria playing numerous key roles. One function of the release of OMVs is related to an increase in surface hydrophobicity. This phenomenon initiates biofilm formation, making bacteria more tolerant to environmental stressors. Read More

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http://dx.doi.org/10.1007/s00253-019-09812-0DOI Listing

Novel metagenome-derived ornithine lipids identified by functional screening for biosurfactants.

Appl Microbiol Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

Institute for Microbial Biotechnology and Metagenomics, Faculty of Natural Sciences, University of the Western Cape, Cape Town, 7535, South Africa.

Biosurfactants are amphiphilic molecules that interact with the surfaces of liquids leading to many useful applications. Most biosurfactants have been identified from cultured microbial sources, leaving a largely untapped resource of uncultured bacteria with potentially novel biosurfactant structures. To access the uncultured bacteria, a metagenomic library was constructed in Escherichia coli from environmental DNA within an E. Read More

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http://link.springer.com/10.1007/s00253-019-09768-1
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http://dx.doi.org/10.1007/s00253-019-09768-1DOI Listing
April 2019
5 Reads

Roles of three TonB systems in the iron utilization and virulence of the Aeromonas hydrophila Chinese epidemic strain NJ-35.

Appl Microbiol Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.

The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the role of these TonB systems in A. Read More

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http://link.springer.com/10.1007/s00253-019-09757-4
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http://dx.doi.org/10.1007/s00253-019-09757-4DOI Listing
April 2019
3 Reads

A novel method: using an adenosine triphosphate (ATP) luminescence-based assay to rapidly assess the biological stability of drinking water.

Appl Microbiol Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

College of Civil Engineering and Architecture, Zhejiang University, Hangzhou, 310058, Zhejiang, China.

The rapid and credible evaluations of the microbial stability of a drinking water distribution system (DWDS) are of great significance for ensuring the safety of drinking water and predicting microbial pollution. Conventional biostability assessment methods mainly focus on bacterial regrowth or evaluation of the level of nutrients that support bacterial regrowth. However, such methods are time-consuming and have many limitations. Read More

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http://dx.doi.org/10.1007/s00253-019-09774-3DOI Listing

Advancing biomarkers for anaerobic o-xylene biodegradation via metagenomic analysis of a methanogenic consortium.

Appl Microbiol Biotechnol 2019 Apr 9. Epub 2019 Apr 9.

Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, CO, USA.

Quantifying functional biomarker genes and their transcripts provides critical lines of evidence for contaminant biodegradation; however, accurate quantification depends on qPCR primers that contain no, or minimal, mismatches with the target gene. Developing accurate assays has been particularly challenging for genes encoding fumarate-adding enzymes (FAE) due to the high level of genetic diversity in this gene family. In this study, metagenomics applied to a field-derived, o-xylene-degrading methanogenic consortium revealed genes encoding FAE that would not be accurately quantifiable by any previously available PCR assays. Read More

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http://dx.doi.org/10.1007/s00253-019-09762-7DOI Listing

Efficient synthesis of an antiviral drug intermediate using an enhanced short-chain dehydrogenase in an aqueous-organic solvent system.

Appl Microbiol Biotechnol 2019 Apr 9. Epub 2019 Apr 9.

Microbial Pharmacology Laboratory, Shanghai University of Medicine and Health Sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.

(2R,3S)-N-tert-Butoxycarbonyl-3-amino-1-chloro-2-hydroxy-4-phenylbutane (1b) is key for the synthesis of the antiviral drug atazanavir. It can be obtained via the stereoselective bioreduction of (3S)-3-(N-Boc-amino)-1-chloro-4-phenyl-butanone (1a) with short-chain dehydrogenase/reductase (SDR). However, the stereoselective bioreduction of this hydrophobic and bulky substrate still remained a challenge because of the steric hindrance effect and low mass transfer rate. Read More

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http://dx.doi.org/10.1007/s00253-019-09781-4DOI Listing

Production of controlled molecular weight hyaluronic acid by glucostat strategy using recombinant Lactococcus lactis cultures.

Appl Microbiol Biotechnol 2019 Apr 9. Epub 2019 Apr 9.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, India.

Hyaluronic acid (HA) is a biopolymer with wide biomedical and cosmetic applications, wherein the molecular weight of HA (MW) is an important quality parameter that determines its suitability for the targeted application. To produce HA with desired molecular weight, it is important to identify parameters that offer tunability and control of MW at a desired value during fermentation. In this work, two tunable parameters, viz. Read More

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http://link.springer.com/10.1007/s00253-019-09769-0
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http://dx.doi.org/10.1007/s00253-019-09769-0DOI Listing
April 2019
3 Reads

Improvement of stress tolerance and riboflavin production of Bacillus subtilis by introduction of heat shock proteins from thermophilic bacillus strains.

Appl Microbiol Biotechnol 2019 Apr 9. Epub 2019 Apr 9.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.

In this study, stress tolerance devices consisting of heat shock protein (HSP) genes from thermophiles Geobacillus and Parageobacillus were introduced into riboflavin-producing strain Bacillus subtilis 446 to improve its stress tolerance and riboflavin production. The 12 HSP homologs were selected from 28 Geobacillus and Parageobacillus genomes according to their sequence clustering and phylogenetically analysis which represents the diversity of HSPs from thermophilic bacillus. The 12 HSP genes and 2 combinations of them (PtdnaK-PtdnaJ-PtgrpE and PtgroeL-PtgroeS) were heterologously expressed in B. Read More

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http://dx.doi.org/10.1007/s00253-019-09788-xDOI Listing

Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database.

Appl Microbiol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden.

Lignin is a heterogeneous aromatic biopolymer and a major constituent of lignocellulosic biomass, such as wood and agricultural residues. Despite the high amount of aromatic carbon present, the severe recalcitrance of the lignin macromolecule makes it difficult to convert into value-added products. In nature, lignin and lignin-derived aromatic compounds are catabolized by a consortia of microbes specialized at breaking down the natural lignin and its constituents. Read More

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http://dx.doi.org/10.1007/s00253-019-09692-4DOI Listing

Mutations in AraR leading to constitutive of arabinolytic genes in Aspergillus niger under derepressing conditions.

Appl Microbiol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.

The AraR transcription factor of Aspergillus niger encodes a Zn(II)Cys transcription factor required for the induction of genes encoding arabinolytic enzymes. One of the target genes of AraR is abfA, encoding an arabinofuranosidase. The expression of abfA as well as other L-arabinose-induced genes in A. Read More

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http://dx.doi.org/10.1007/s00253-019-09777-0DOI Listing
April 2019
3 Reads

Beer yeast-derived fluorescent carbon dots for photoinduced bactericidal functions and multicolor imaging of bacteria.

Appl Microbiol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

Beer yeast-modified fluorescent carbon dots were synthesized via a one-step strategy for photoinduced bactericidal functions and bio-imaging in bacterial viability assessment. The proposed carbon dots (CDs) were used as an visible light-triggered antibacterial material, and the antimicrobial activities of the CDs against Gram-negative model bacterial species (Escherichia coli) were evaluated under conditions of varying other experimental parameters including CDs concentrations and treatment times. The result showed that the CDs have excellent antibacterial performance of bactericidal effect within 120 min of under visible-light irradiation. Read More

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http://dx.doi.org/10.1007/s00253-019-09782-3DOI Listing

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields.

Appl Microbiol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan.

Enzymatic hydrolysis of polyethylene terephthalate (PET) has been the subject of extensive previous research that can be grouped into two categories, viz. enzymatic surface modification of polyester fibers and management of PET waste by enzymatic hydrolysis. Different enzymes with rather specific properties are required for these two processes. Read More

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http://dx.doi.org/10.1007/s00253-019-09717-yDOI Listing

Enhancing the atypical esterase promiscuity of the γ-lactamase Sspg from Sulfolobus solfataricus by substrate screening.

Appl Microbiol Biotechnol 2019 Apr 6. Epub 2019 Apr 6.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.

Promiscuous enzymes can be modified by protein engineering, which enables the catalysis of non-native substrates. γ-lactamase Sspg from Sulfolobus solfataricus is an enzyme with high activity, high stability, and pronounced tolerance of high concentrations of the γ-lactam substrate. These characteristics suggest Sspg as a robust enzymatic catalyst for the preparation of optically pure γ-lactam. Read More

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http://dx.doi.org/10.1007/s00253-019-09758-3DOI Listing
April 2019
1 Read

Effect of the spoIIID mutation on mother cell lysis in Bacillus thuringiensis.

Appl Microbiol Biotechnol 2019 Apr 5. Epub 2019 Apr 5.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

SpoIIID is a small, sequence-specific DNA-binding protein which can direct many genes' transcription and has an effect on spore formation in Bacillus subtilis. We investigated the role of SpoIIID in mother cell lysis in Bacillus thuringiensis. A β-galactosidase assay based on the promoter fusions with lacZ indicated that the sigK gene was positively regulated by SpoIIID and σ negatively regulated the expression of sigE. Read More

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http://dx.doi.org/10.1007/s00253-019-09722-1DOI Listing
April 2019
1 Read
3.337 Impact Factor

Enhancement of substrate supply and ido expression to improve 4-hydroxyisoleucine production in recombinant Corynebacterium glutamicum ssp. lactofermentum.

Appl Microbiol Biotechnol 2019 Apr 5. Epub 2019 Apr 5.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.

4-Hydroxyisoleucine (4-HIL) has potential value in treating diabetes. L-isoleucine dioxygenase (IDO) catalyzes the hydroxylation of L-isoleucine (Ile) to form 4-HIL with the concomitant oxidation of α-ketoglutarate (α-KG) and oxygen consumption. In our previous study, by expressing the ido gene in the Ile producer Corynebacterium glutamicum ssp. Read More

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http://link.springer.com/10.1007/s00253-019-09791-2
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http://dx.doi.org/10.1007/s00253-019-09791-2DOI Listing
April 2019
2 Reads

Functional analysis of putative transporters involved in oligotrophic growth of Rhodococcus erythropolis N9T-4.

Appl Microbiol Biotechnol 2019 Apr 5. Epub 2019 Apr 5.

Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, 432-8561, Japan.

Rhodococcus erythropolis N9T-4, which is an extremely oligotrophic bacterium, can survive in a completely inorganic medium with no additional carbon source. This bacterium utilizes atmospheric CO, but does not require any additional energy source such as light and hydrogen gas, required by autotrophic microorganisms. However, its CO fixation and energy-acquisition systems in the oligotrophic growth remain unrevealed. Read More

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http://dx.doi.org/10.1007/s00253-019-09714-1DOI Listing
April 2019
2 Reads

Effect of stock density on the microbial community in biofloc water and Pacific white shrimp (Litopenaeus vannamei) gut microbiota.

Appl Microbiol Biotechnol 2019 Apr 5. Epub 2019 Apr 5.

Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.

Biofloc technology is an efficient approach for intensive shrimp culture. However, the extent to which this process can influence the composition of intestinal microbial community is still unknown. Here, we surveyed the shrimp intestinal bacteria as well as the floc water from three biofloc systems with different stock densities. Read More

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http://link.springer.com/10.1007/s00253-019-09773-4
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http://dx.doi.org/10.1007/s00253-019-09773-4DOI Listing
April 2019
3 Reads

Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production.

Appl Microbiol Biotechnol 2019 Apr 4. Epub 2019 Apr 4.

Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, Coupure Links 653, 9000, Ghent, Oost-Vlaanderen, Belgium.

Both strain engineering and process optimization are intensively studied in microbial biosurfactant literature. However, screening of multiple strains and/or medium components in parallel is a very labor-intensive and timely process, considering the only applied technique nowadays is evaluation through shake flask and/or bioreactor experiments. Therefore, in this work, the development, optimization, and application of a more throughput technique-based on 24-deep well plates-are described for a new Starmerella bombicola strain producing bolaform sophorolipids. Read More

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http://link.springer.com/10.1007/s00253-019-09766-3
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http://dx.doi.org/10.1007/s00253-019-09766-3DOI Listing
April 2019
2 Reads

Enzyme kinetics of fungal glucuronoyl esterases on natural lignin-carbohydrate complexes.

Appl Microbiol Biotechnol 2019 Apr 4. Epub 2019 Apr 4.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 221, DK-2800, Kongens Lyngby, Denmark.

Glucuronoyl esterases (CE15 family) enable targeted cleavage of ester linkages in lignin-carbohydrate complexes (LCCs), particularly those linking lignin and glucuronoyl residues in xylan. A substantial challenge in characterization and kinetic analysis of CE15 enzymes has been the lack of proper substrates. Here, we present an assay using an insoluble LCC-rich lignin fraction from birch; lignin-rich pellet (LRP). Read More

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http://dx.doi.org/10.1007/s00253-019-09797-wDOI Listing
April 2019
1 Read

Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746.

Appl Microbiol Biotechnol 2019 Apr 4. Epub 2019 Apr 4.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People's Republic of China.

Bacillus spp. are important producers of bioactive natural products with potential applications in medicine and agriculture. Bacillus sp. Read More

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http://dx.doi.org/10.1007/s00253-019-09759-2DOI Listing

Electrolyzing lactic acid in situ in fermentation broth to produce pyruvic acid in electrolysis cell.

Appl Microbiol Biotechnol 2019 Apr 3. Epub 2019 Apr 3.

School of Environmental Engineering, Wuhan Textile University, 430073, Wuhan, People's Republic of China.

Pyruvic acid is an important chemical in the carboxylate platform. Obstacles for its implementation are the need for high energy in chemical synthesis and additives in fermentation leading to increased production costs. Here, pyruvic acid generation from direct conversion of lactic acid in fermentation broth by electrolysis method is presented. Read More

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http://dx.doi.org/10.1007/s00253-019-09793-0DOI Listing
April 2019
1 Read

Remarkable diversification of bacterial azoreductases: primary sequences, structures, substrates, physiological roles, and biotechnological applications.

Authors:
Hirokazu Suzuki

Appl Microbiol Biotechnol 2019 Apr 3. Epub 2019 Apr 3.

Faculty of Engineering, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8552, Japan.

Azoreductases reductively cleave azo linkages by using NAD(P)H as an electron donor. The enzymes are widely found in bacteria and act on numerous azo dyes, which allow various unique applications. This review describes primary amino acid sequences, structures, substrates, physiological roles, and biotechnological applications of bacterial azoreductases to discuss their remarkable diversification. Read More

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http://dx.doi.org/10.1007/s00253-019-09775-2DOI Listing
April 2019
2 Reads

Fungal biotransformation of short-chain n-alkylcycloalkanes.

Appl Microbiol Biotechnol 2019 Apr 2. Epub 2019 Apr 2.

Institute of Microbiology, University of Greifswald, Felix-Hausdorff-Strasse 8, 17489, Greifswald, Germany.

The cycloalkanes, comprising up to 45% of the hydrocarbon fraction, occur in crude oil or refined oil products (e.g., gasoline) mainly as alkylated cyclohexane derivatives and have been increasingly found in environmental samples of soil and water. Read More

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http://dx.doi.org/10.1007/s00253-019-09749-4DOI Listing
April 2019
3 Reads

Gut bacteria of animals/pests living in polluted environments are a potential source of antibacterials.

Appl Microbiol Biotechnol 2019 Apr 2. Epub 2019 Apr 2.

Department of Biological Sciences, School of Science and Technology, Sunway University, 47500, Petaling Jaya, Selangor, Malaysia.

The morbidity and mortality associated with bacterial infections have remained significant despite chemotherapeutic advances. With the emergence of drug-resistant bacterial strains, the situation has become a serious threat to the public health. Thus, there is an urgent need to identify novel antibacterials. Read More

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http://dx.doi.org/10.1007/s00253-019-09783-2DOI Listing
April 2019
2 Reads