22,700 results match your criteria Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry[Journal]


A novel knitted scaffold made of microfiber/nanofiber core-sheath yarns for tendon tissue engineering.

Biomater Sci 2020 Jul 10. Epub 2020 Jul 10.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Tendon injury is common in sports and other rigorous activities, which may result in dysfunction and disability. Recently, scaffolds with a knitted structure have been widely applied for tendon tissue engineering. The purpose of this study was to fabricate a novel knitted tendon scaffold made of microfiber/nanofiber core-sheath yarns and evaluate the biocompatibility and the effect of tenogenic differentiation and tendon tissue regeneration in vitro and in vivo. Read More

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http://dx.doi.org/10.1039/d0bm00816hDOI Listing

[Effect of electroacupuncture on expressions of Lyn and Syk in mast cells of subcutaneous loose connective tissue in rats with urticarial].

Zhongguo Zhen Jiu 2020 Jul;40(7):765-70

Graduate School, Liaoning University of TCM, Shenyang 110032, China.

Objective: To observe the effect of electroacupuncture (EA) preconditioning on the expressions of tyrosine kinase Lyn and spleen tyrosine kinase (Syk) in mast cells of subcutaneous loose connective tissue in the rats with urticaria and explore the potential biological mechanism of EA in the intervention of urticaria.

Methods: A total of 32 SD rats were randomized into a blank group, a model group, an EA group and a positive medication group, 8 rats in each one. Except of the blank group, the passive cutaneous anaphylaxis (PCA) was adopted to prepare the model of urticaria in the rats of the rest three groups. Read More

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http://dx.doi.org/10.13703/j.0255-2930.20191023-k0005DOI Listing

Genetic contribution of endoplasmic reticulum aminopeptidase 1 polymorphisms to liver fibrosis progression in patients with HCV infection.

J Mol Med (Berl) 2020 Jul 9. Epub 2020 Jul 9.

Translational Immunology Laboratory, Health Research Institute of the Principality of Asturias (ISPA), Hospital Universitario Central de Asturias, Oviedo, Spain.

The endoplasmic reticulum aminopeptidase ERAP1 regulates innate and adaptive immune responses, trimming peptides and loading onto HLA class I molecules. Coding single nucleotide polymorphisms within ERAP1 are associated with autoimmune diseases, viral infections, and cancer development. Our purpose was to analyze the influence of ERAP1 variants on fibrogenesis in hepatitis C virus (HCV)-infected patients. Read More

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http://dx.doi.org/10.1007/s00109-020-01948-1DOI Listing

Morphological and neurochemical characterisation of anterogradely labelled spinal sensory and autonomic nerve endings in the mouse bladder.

Auton Neurosci 2020 Jul 1;227:102697. Epub 2020 Jul 1.

Discipline of Human Physiology, Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Adelaide, Australia. Electronic address:

The bladder is innervated by axons of sympathetic and parasympathetic efferent nerves, and by spinal afferent neurons. The objective was to characterise anatomically and immunohistochemically the terminal endings of sensory and autonomic motor nerve endings in wholemount preparations of the mouse bladder. We used both anterograde labelling of pelvic and hypogastric nerves ex vivo and anterograde labelling from lumbosacral dorsal root ganglia (DRG) in vivo in male and female mice. Read More

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http://dx.doi.org/10.1016/j.autneu.2020.102697DOI Listing

Switches of SOX17 and SOX2 expression in the development of squamous metaplasia and squamous intraepithelial lesions of the uterine cervix.

Cancer Med 2020 Jul 9. Epub 2020 Jul 9.

Department of Molecular Cell Biology, GROW School for Oncology & Developmental Biology, Maastricht University Medical Centre, Maastricht, The Netherlands.

Aims: The dynamics and topographical distribution of SOX17 and SOX2 expression was studied in the transformation zone (TZ) of the uterine cervix. This TZ is a dynamic area where switches from glandular into squamous epithelium can be recognized, new squamocolumnar junctions are formed, and premalignant lesions originate. SOX17 and SOX2 show mutually exclusive expression patterns in the normal uterine cervix, with SOX2 being exclusively found in squamous epithelium, while SOX17 is detected in endocervical columnar cells and reserve cells. Read More

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http://dx.doi.org/10.1002/cam4.3201DOI Listing

Role of CD68 immunohistochemistry in categorizing benign nonmesothelial cell population and refining "atypical" category in serous fluid cytology.

Diagn Cytopathol 2020 Jul 9. Epub 2020 Jul 9.

Department of Pathology, MetroHealth Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.

Background: Body fluids are rich in histiocytes and may mimic atypical epithelial cells morphologically. Histiocytes can pose a significant challenge in serous fluid cytology as they tend to appear atypical due to prolonged accumulation in serous fluids in vivo and processing by liquid-based cytology in vitro. Not many studies have documented the utilization of histiocytic marker such as CD68 in serous fluid cytology, which can subsequently reduce the "atypical" diagnostic category. Read More

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http://dx.doi.org/10.1002/dc.24541DOI Listing

[Effect of catgut implantation at "Yingxiang"(LI20) on lower airway remodeling in allergic rhinitis rats].

Zhen Ci Yan Jiu 2020 Jun;45(6):461-7

Department of Pneumology, Shuangliu Hospital of Traditional Chinese Medicine, Chengdu 610000.

Objective: To observe the effect of catgut implantation at "Yingxiang"(LI20) on lower airway remodeling and levels of osteopon-tin (OPN) protein in allergic rhinitis (AR) rats, so as to reveal its mechanisms underlying improvement of AR.

Methods: Male SD rats were randomly divided into control, model and catgut implantation groups, with 10 rats in each group. The AR model was established by intraperitoneal injection and nasal drip of ovalbumin. Read More

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http://dx.doi.org/10.13702/j.1000-0607.190802DOI Listing

[Effect of electroacupuncture on recognition memory and levels of Aβ, inflammatory factor proteins and aquaporin 4 in hippocampus of APP/PS1 double transgenic mice].

Zhen Ci Yan Jiu 2020 Jun;45(6):431-7

School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Objective: To investigate the effect of electroacupuncture (EA) at "Baihui "(GV20) and "Shenshu "(BL23) on activation of glial cells, expression of inflammatory factor proteins and aquaporin 4 (AQP4)in the hippocampus of amyloid precursor protein/presenilin-1 (APP/PS1) transgenic mice, so as to explore its mechanisms underlying improvement of Alzheimer's disease(AD).

Methods: Twenty C57/BL6 background male APP695/PS1-dE9(APP/PS1) double transgenic mice (model group) and 20 wild type (WT) C57/BL6 mice (blank group) were respectively randomized into control and EA groups. EA (2 Hz/15 Hz, 1-2 mA) was applied to GV20 and bilateral BL23 for 30 min, once daily, 6 days a week for 4 weeks. Read More

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http://dx.doi.org/10.13702/j.1000-0607.190923DOI Listing

High levels of KLK7 protein expression are related to a favorable prognosis in triple-negative breast cancer patients.

Am J Cancer Res 2020 1;10(6):1785-1792. Epub 2020 Jun 1.

Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich (TUM) Germany.

In normal physiology, kallikrein-related peptidase 7 (KLK7), together with other members of the kallikrein-related peptidase family, is mainly involved in skin desquamation and keratinization processes. Moreover, expression of KLK7 was shown in various tumor types to be dysregulated and to correlate to patients' survival time. However, there are contradictory reports in breast cancer whether KLK7 represents an unfavorable or favorable prognostic biomarker. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339269PMC

Cellular Heterogeneity Landscape in Laryngeal Squamous Cell Carcinoma.

Int J Cancer 2020 Jul 7. Epub 2020 Jul 7.

Department of Microbiology, Harbin Medical University, Harbin, China.

Laryngeal squamous cell carcinoma (LSCC) is a highly malignant tumor originated from respiratory system. Although there have been many improvements in therapy until now, reducing the high mortality is still difficult. Understanding the cellular heterogeneity of LSCC could contribute to improve this problem. Read More

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http://dx.doi.org/10.1002/ijc.33192DOI Listing

Synergistic effects of pazopanib and hyperthermia against uterine leiomyosarcoma growth mediated by downregulation of histone acetyltransferase 1.

J Mol Med (Berl) 2020 Jul 7. Epub 2020 Jul 7.

Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University, College of Medicine, 5 Fushin St., Guishan, Taoyuan, 333, Taiwan.

Pazopanib-a multitargeted tyrosine kinase inhibitor with prominent antiangiogenic effects-has shown promise in the treatment of soft-tissue sarcomas. Hyperthermia has been also applied as an adjunctive treatment to chemotherapy for these malignancies. Here, we show that pazopanib and hyperthermia act synergistically in inhibiting uterine leiomyosarcoma (LMS) cell growth. Read More

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http://dx.doi.org/10.1007/s00109-020-01888-wDOI Listing

Hyperspectral and multispectral imaging in digital and computational pathology: a systematic review [Invited].

Biomed Opt Express 2020 Jun 21;11(6):3195-3233. Epub 2020 May 21.

Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.

Hyperspectral imaging (HSI) and multispectral imaging (MSI) technologies have the potential to transform the fields of digital and computational pathology. Traditional digitized histopathological slides are imaged with RGB imaging. Utilizing HSI/MSI, spectral information across wavelengths within and beyond the visual range can complement spatial information for the creation of computer-aided diagnostic tools for both stained and unstained histological specimens. Read More

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http://dx.doi.org/10.1364/BOE.386338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315999PMC

Immunohistochemistry-Based Taxonomical Classification of Bladder Cancer Predicts Response to Neoadjuvant Chemotherapy.

Cancers (Basel) 2020 Jul 3;12(7). Epub 2020 Jul 3.

Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 28029 Madrid, Spain.

Background: Platinum-based neoadjuvant chemotherapy (NAC) increases the survival of patients with organ-confined urothelial bladder cancer (UBC). In retrospective studies, patients with basal/squamous (BASQ)-like tumors present with more advanced disease and have worse prognosis. Transcriptomics-defined tumor subtypes are associated with response to NAC. Read More

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http://dx.doi.org/10.3390/cancers12071784DOI Listing

PTEN and ERG expression in MRI-ultrasound guided fusion biopsy correlated with radical prostatectomy findings in men with prostate cancer.

Prostate 2020 Jul 7. Epub 2020 Jul 7.

Department of Pathology (HUS Diagnostic Center) and Medicum, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.

Background: Conventional systematic prostate biopsies (SBx) have multiple limitations, and magnetic resonance imaging (MRI)-ultrasound fusion targeting is increasingly applied (fusion biopsies [FBx]). In our previous studies, we have shown that loss of the tumor suppressor gene phosphatase and tensin homolog (PTEN) in radical prostatectomy (RP) specimens predicts poor disease-specific survival, and in active surveillance (AS), PTEN loss in SBx predicts an adverse AS outcome, although SBx PTEN status does not correlate well with the corresponding RP status. Here, we have hypothesized that PTEN and erythroblast transformation-specific related gene (ERG) status in FBx correlate better with RP than they would in SBx. Read More

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http://dx.doi.org/10.1002/pros.24040DOI Listing

Molecular MRI of the Immuno-Metabolic Interplay in a Rabbit Liver Tumor Model: A Biomarker for Resistance Mechanisms in Tumor-targeted Therapy?

Radiology 2020 Jul 7:200373. Epub 2020 Jul 7.

From the Department of Radiology and Biomedical Imaging (L.J.S., L.A.D., I.T.S., J.J.W., J.S., M.D.L., L.A., A.B., J.D., F.H., D.C., J.C.), Department of Internal Medicine, Section of Rheumatology (R.R.M., L.L., R.J.B.), Department of Immunobiology (N.J.), and Department of Pathology (V.P., X.Z.), Yale University School of Medicine, 300 Cedar St, New Haven, CT 06520; Institute of Radiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität, and Berlin Institute of Health, Berlin, Germany (L.J.S., L.A.D., I.T.S., L.A.); Visage Imaging, San Diego, Calif (M.D.L.); Department of Biomedical Engineering, Yale School of Engineering and Applied Science, New Haven, Conn (J.D.); and Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel (S.N.G.).

Background The immuno-metabolic interplay has gained interest for determining and targeting immunosuppressive tumor micro-environments that remain a barrier to current immuno-oncologic therapies in hepatocellular carcinoma. Purpose To develop molecular MRI tools to reveal resistance mechanisms to immuno-oncologic therapies caused by the immuno-metabolic interplay in a translational liver cancer model. Materials and Methods A total of 21 VX2 liver tumor-bearing New Zealand white rabbits were used between October 2018 and February 2020. Read More

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http://dx.doi.org/10.1148/radiol.2020200373DOI Listing

Prevalence of Helicobacter pylori among Sudanese patients diagnosed with colon polyps and colon cancer using immunohistochemistry technique.

BMC Res Notes 2020 Jul 6;13(1):322. Epub 2020 Jul 6.

Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

Objectives: Infection with the bacteria Helicobacter pylori has been classified as class one carcinogen associated with increasing susceptibility of gastritis and gastric carcinoma. This study is aiming at investigating the prevalence of H. pylori among colon polyps and colon cancer patients. Read More

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http://dx.doi.org/10.1186/s13104-020-05159-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339555PMC

miR-196a-mediated downregulation of p27 protein promotes prostate cancer proliferation and relates to biochemical recurrence after radical prostatectomy.

Prostate 2020 Jul 6. Epub 2020 Jul 6.

Department of Urology, Fujian Medical University Union Hospital, Fuzhou, China.

Background: Dysregulation of microRNAs has performed vital gene regulatory functions in the genesis, progression, and prognosis of multiple malignant tumors. This study aimed to elucidate the regulatory mechanism of miR-196a in prostate cancer (PCa) and explore its clinical significance.

Methods: Quantitative real-time polymerase chain reaction was implemented to examine miR-196a and p27 messenger RNA expression in PCa. Read More

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http://dx.doi.org/10.1002/pros.24036DOI Listing

Deficiency of Intestinal α1-2-fucosylation Exacerbates Ethanol-Induced Liver Disease in Mice.

Alcohol Clin Exp Res 2020 Jul 6. Epub 2020 Jul 6.

Department of Medicine, University of California San Diego, La Jolla, CA, USA, La Jolla.

Background: Fucosyltransferase 2 (Fut2)-mediated intestinal α1-2-fucosylation is important in maintaining a symbiotic host-microbiota relationship and can protect against several pathogens. Intestinal dysbiosis is an important factor for the progression of experimental ethanol-induced liver disease, but the role of Fut2 in modulating the intestinal glycocalyx during alcohol-associated liver disease is unknown. We investigated the role of Fut2-mediated intestinal α1-2-fucosylation for the development of alcohol-associated liver disease. Read More

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http://dx.doi.org/10.1111/acer.14405DOI Listing

IL‑18 knockout alleviates atopic dermatitis‑like skin lesions induced by MC903 in a mouse model.

Int J Mol Med 2020 Aug 4;46(2):880-888. Epub 2020 Jun 4.

Graduate School, China Medical University, Shenyang, Liaoning 110001, P.R. China.

Interleukin (IL)‑18, a pro‑inflammatory cytokine, plays an important role in a number of skin diseases. The aim of the present study was to investigate the role of IL‑18 in the development of atopic dermatitis (AD). For this purpose, mice were divided into 4 groups (n=5/group) as follows: i) The wild‑type (WT) controls; ii) IL18 knockout (KO) controls; iii) MC903‑treated WT mice; and iv) MC903‑treated KO mice. Read More

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http://dx.doi.org/10.3892/ijmm.2020.4630DOI Listing

Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver.

Acta Histochem Cytochem 2020 Jun 29;53(3):55-60. Epub 2020 May 29.

Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.

Alogliptin is one of a new class of therapeutic agents for type 2 diabetes called dipeptidyl peptidase-4 inhibitors. Here, we used immunohistochemistry to investigate the pharmacokinetics of alogliptin at the cell and tissue levels in the rat kidney and liver. One hour after alogliptin administration, the most noticeable immunoreactivity in the kidney was a moderate-to-strong staining in proximal tubule S3 segment epithelial cells. Read More

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http://dx.doi.org/10.1267/ahc.19036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322161PMC

Identification of aspartame-induced haematopoietic and lymphoid tumours in rats after lifetime treatment.

Acta Histochem 2020 Jul 20;122(5):151548. Epub 2020 May 20.

Cesare Maltoni Cancer Research Center, Ramazzini Institute, via Saliceto 3, 40010 Bentivoglio, Bologna, Italy. Electronic address:

Lymphomas and leukaemias involving the lung have in some cases been hard to distinguish from respiratory tract infection in Sprague-Dawley (SD) rats from long-term bioassays. In order to differentiate between tumours and immune cell infiltrates, updated pathological criteria and nomenclature were used and immunohistochemistry (IHC) was applied to haematopoietic and lymphoid tissue tumours (HLTs) in the original prenatal long-term Aspartame (APM) study performed by the Ramazzini Institute (RI). All 78 cases of HLTs from treated and control groups were re-examined based on light microscopic morphological characteristics and subjected to a panel of IHC markers including Ki67, CD3, PAX5, CD20, CD68, TdT, CD45, CD14 and CD33. Read More

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http://dx.doi.org/10.1016/j.acthis.2020.151548DOI Listing

Epigallocatechin-3-gallate (EGCG) reduces apoptosis of preantral follicles through the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) signaling pathway after in vitro culture of sheep ovarian tissue.

Theriogenology 2020 Jun 4;155:25-32. Epub 2020 Jun 4.

Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.

The aims of this study were to analyze the effects of different concentrations of epigallocatechin-3-gallate (EGCG) on the primordial follicle survival and development after in vitro culture of ovarian tissue, and to verify the possible involvement of the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) pathway in the EGCG actions in the sheep ovary. Ovarian fragments were fixed for histological analysis (fresh control) or cultured in α-minimum essential medium alone (α-MEM: control medium) or with different concentrations of EGCG (0.01; 0. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2020.05.037DOI Listing

Exosome-mediated delivery of miR-204-5p inhibits tumor growth and chemoresistance.

Cancer Med 2020 Jul 2. Epub 2020 Jul 2.

Wuxi Cancer Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.

Background: Nano-sized extracellular vesicles secreted by cells play key roles in intercellular crosstalk, and appear to be an excellent biocompatible material as therapeutic cargoes in vivo. Previously, we have demonstrated that miR-204-5p is a key tumor suppressor that could inhibit tumor growth, metastasis and chemoresistance.

Methods: A HEK293T cell line stably expressing miR-204-5p (293T-miR-204) was constructed by lentivirus transduction. Read More

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http://dx.doi.org/10.1002/cam4.3248DOI Listing

MR-Based Electrical Conductivity Imaging of Liver Fibrosis in an Experimental Rat Model.

J Magn Reson Imaging 2020 Jul 2:e27275. Epub 2020 Jul 2.

Department of Biomedical Engineering, Kyung Hee University, Seoul, 02447, Korea.

Background: Liver fibrosis is characterized by the excessive accumulation of extracellular matrix proteins. Electrical conductivity imaging at low frequency can provide novel contrast because the contrast mechanisms originate from the changes in the concentration and mobility of ions in the extracellular space.

Purpose: To evaluate the feasibility of an MR-based electrical conductivity imaging that can detect the changes in a tissue condition associated with the progression of liver fibrosis. Read More

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http://dx.doi.org/10.1002/jmri.27275DOI Listing

Combined lectin- and immuno-histochemistry (CLIH) for applications in cell biology and cancer diagnosis: Analysis of human urothelial carcinomas.

Eur J Histochem 2020 Jul 1;64(3). Epub 2020 Jul 1.

Institute of Cell Biology, Faculty of Medicine, University of Ljubljana.

Lectin histochemistry (LHC) and immunohistochemistry (IHC), which demonstrate the composition and localisation of sugar residues and proteins in cell membranes, respectively, are generally used separately. Using these two methods, we previously demonstrated that malignant transformation of urothelial cells results in the alterations of protein glycosylation and reduced expression of urothelium-specific integral membrane proteins uroplakins (UPs). However, the correlation between these changes was not studied yet. Read More

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http://dx.doi.org/10.4081/ejh.2020.3141DOI Listing

A Melanin bleaching method to prevent non-specific immunostaining of chicken feathers.

MethodsX 2020 11;7:100957. Epub 2020 Jun 11.

Animal Science, School of Environmental and Rural Science, University of New England, Australia.

Melanin in pigmented organs like the skin is known to react with 3,3'-diaminobenzidine (DAB) to give a brown colour indistinguishable from the colour that DAB imparts to target antibodies bound to specific antigens. This can lead to false positives in chicken feathers during immunoperoxidase staining. Here, we present a simple, fast and practical method for bleaching chicken feathers which can be applied prior to immunohistochemistry staining without affecting specific antigen-antibody binding. Read More

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http://dx.doi.org/10.1016/j.mex.2020.100957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317669PMC

The diagnosis of primary thyroid lymphoma by fine-needle aspiration, cell block, and immunohistochemistry technique.

Diagn Cytopathol 2020 Jul 1. Epub 2020 Jul 1.

Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Aim: Primary thyroid lymphoma (PTL) is a rare malignant disease. Its prognosis depends on early diagnosis. The role of fine-needle aspiration (FNA), including smear cytology, cell block (CB) techniques, and immunohistochemistry (IHC) sections in the diagnosis of PTL is still unclear. Read More

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http://dx.doi.org/10.1002/dc.24526DOI Listing

Recovery of motor function after traumatic spinal cord injury by using plasma-synthesized polypyrrole/iodine application in combination with a mixed rehabilitation scheme.

J Mater Sci Mater Med 2020 Jun 30;31(7):58. Epub 2020 Jun 30.

Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI. Av. Cuauhtémoc 330, Col. Doctores, México City, CP, 06720, México.

Traumatic spinal cord injury (TSCI) can cause paralysis and permanent disability. Rehabilitation (RB) is currently the only accepted treatment, although its beneficial effect is limited. The development of biomaterials has provided therapeutic possibilities for TSCI, where our research group previously showed that the plasma-synthesized polypyrrole/iodine (PPy/I), a biopolymer with different physicochemical characteristics than those of the PPy synthesized by conventional methods, promotes recovery of motor function after TSCI. Read More

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http://dx.doi.org/10.1007/s10856-020-06395-5DOI Listing

Upregulated SET Promotes Cell Survival Through Activating Akt/NF-κB Signal in Colorectal Carcinoma.

Cancer Manag Res 2020 19;12:4735-4745. Epub 2020 Jun 19.

Department of Medical Cellular Biology and Genetics, Shanxi Medical University, Taiyuan 030001, People's Republic of China.

Purpose: SET has been proven to be an oncogene, which promotes the initiation and progression in several kinds of malignant carcinomas. However, the expression and its functional roles in colorectal carcinoma (CRC) remained unknown.

Materials And Methods: CRC tissues samples, CRC cell lines and xenograft mouse tumors were used in this study. Read More

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http://dx.doi.org/10.2147/CMAR.S255930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310974PMC

Ki67 and CD31 Differential Expression in Cutaneous T-Cell Lymphoma and Its Mimickers: Association with Clinicopathological Criteria and Disease Advancement.

Clin Cosmet Investig Dermatol 2020 23;13:431-442. Epub 2020 Jun 23.

Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Background: Cell proliferation and angiogenesis are important in progression of cancerous processes. Differentiating cutaneous T-cell lymphoma (CTCL) from its mimicking dermatoses and prognosticating it are challenging.

Aim: This study assesses cell proliferation and angiogenesis in different CTCL subtypes using immunohistochemistry (IHC) for Ki67 and CD31 to testify their usability in differentiating CTCL from mimicking dermatoses and discriminating CTCL subtypes from each other with correlation to clinicopathological parameters and disease advancement. Read More

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http://dx.doi.org/10.2147/CCID.S256269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320895PMC

B7-H6 Promotes Cell Proliferation, Migration and Invasion of Non-Hodgkin Lymphoma via Ras/MEK/ERK Pathway Based on Quantitative Phosphoproteomics Data.

Onco Targets Ther 2020 18;13:5795-5805. Epub 2020 Jun 18.

Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing 100191, People's Republic of China.

Purpose: B7 homologue 6 (B7-H6) has been found at an up-regulated level in multiple cancer cells and identified to be positively correlated with inferior clinical features. In non-Hodgkin lymphoma (NHL), however, the roles of B7-H6 and the underlying mechanism of action remain unclear. Through in vivo and in vitro experiments, the aim of this study was to explore the regulatory mechanism of B7-H6 in NHL in order to provide new therapeutic strategies that can potentially be applied in clinical practice. Read More

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http://dx.doi.org/10.2147/OTT.S257512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308182PMC

Upregulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/β-catenin signaling pathway.

World J Surg Oncol 2020 Jun 30;18(1):147. Epub 2020 Jun 30.

Department of Neurology, Qingdao Central Hospital, Qingdao University, No.127 Siliunan Road, Qingdao, 266042, China.

Background: Mounting evidences displayed that miRNAs play crucial roles in tumor initiation and development. However, the regulation and relevant mechanism of miR-1825 in glioblastoma (GBM) remain unclear.

Methods: qRT-PCR was used to detect miR-1825 and CDK14 mRNA expression. Read More

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http://dx.doi.org/10.1186/s12957-020-01927-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325653PMC

Clinical-Forensic Autopsy Findings to Defeat COVID-19 Disease: A Literature Review.

J Clin Med 2020 Jun 28;9(7). Epub 2020 Jun 28.

Department of Medical, Surgical and Advanced Technologies "G.F. Ingrassia", University of Catania, 95121 Catania, Italy.

The severe acute respiratory syndrome (SARS)-CoV-2 was identified for the first time in China, in December 2019. Confirmed cases of COVID-19 have been reported around the world; indeed, this infection has been declared a pandemic. Consequently, the scientific community is working hard to gain useful information about the history of this virus, its transmission, diagnosis, clinical features, radiological findings, research and development of candidate therapeutics as well as vaccines. Read More

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http://dx.doi.org/10.3390/jcm9072026DOI Listing

Proximity Ligation Assay as a Tool for Antibody Validation in Human Tissues.

J Histochem Cytochem 2020 Jul 30;68(7):515-529. Epub 2020 Jun 30.

Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.

Immunohistochemistry (IHC) is the accepted standard for spatial analysis of protein expression in tissues. IHC is widely used for cancer diagnostics and in basic research. The development of new antibodies to proteins with unknown expression patterns has created a demand for thorough validation. Read More

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http://dx.doi.org/10.1369/0022155420936384DOI Listing

Systematic investigation of the skin in Chst14-/- mice: a model for skin fragility in musculocontractural Ehlers-Danlos syndrome caused by CHST14 variants (mcEDS-CHST14).

Glycobiology 2020 Jun 27. Epub 2020 Jun 27.

Laboratory of Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

Loss-of-function variants in CHST14 cause a dermatan 4-O-sulfotransferase deficiency named musculocontractural Ehlers-Danlos syndrome-CHST14 (mcEDS-CHST14), resulting in complete depletion of the dermatan sulfate moiety of decorin glycosaminoglycan (GAG) chains, which is replaced by chondroitin sulfate. Recently, we uncovered structural alteration of GAG chains in the skin of patients with mcEDS-CHST14. Here, we conducted the first systematic investigation of Chst14 gene-deleted homozygote (Chst14-/-) mice. Read More

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http://dx.doi.org/10.1093/glycob/cwaa058DOI Listing

Ultrasound super-resolution imaging provides a noninvasive assessment of renal microvasculature changes during mouse acute kidney injury.

Kidney Int 2020 Mar 3. Epub 2020 Mar 3.

Department of Bioengineering, University of Pittsburgh School of Engineering, Pittsburgh, Pennsylvania, USA; Center for Ultrasound Molecular Imaging and Therapeutics, Department of Medicine, Heart and Vascular Institute, University of Pittsburgh School of Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA; Division of Cardiology, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; McGowan Institute of Regenerative Medicine, University of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA; Department of Mechanical Engineering and Materials Science, University of Pittsburgh School of Engineering, Pittsburgh, Pennsylvania, USA. Electronic address:

Acute kidney injury (AKI) is a risk factor for the development of chronic kidney disease (CKD). One mechanism for this phenomenon is renal microvascular rarefaction and subsequent chronic impairment in perfusion. However, diagnostic tools to monitor the renal microvasculature in a noninvasive and quantitative manner are still lacking. Read More

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http://dx.doi.org/10.1016/j.kint.2020.02.011DOI Listing

High expression of RABL6 promotes cell proliferation and predicts poor prognosis in esophageal squamous cell carcinoma.

BMC Cancer 2020 Jun 29;20(1):602. Epub 2020 Jun 29.

Department of Thoracic Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, People's Republic of China.

Background: Esophageal squamous cell carcinoma (ESCC) is a common malignant carcinoma of digestive system with high mortality. RAB, member RAS oncogene family like 6 (RABL6), a member of the RAS subfamily, has been reported as an important molecule in several cancers. However, its potential role in ESCC still remains unclear. Read More

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http://dx.doi.org/10.1186/s12885-020-07068-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325041PMC

A Clinically Applicable Approach to the Classification of B-Cell Non-Hodgkin Lymphomas with Flow Cytometry and Machine Learning.

Cancers (Basel) 2020 Jun 24;12(6). Epub 2020 Jun 24.

Laboratory of Immunopathology, Division of Pathology, A.O. Ordine Mauriziano, 10128 Turin, Italy.

The immunophenotype is a key element to classify B-cell Non-Hodgkin Lymphomas (B-NHL); while it is routinely obtained through immunohistochemistry, the use of flow cytometry (FC) could bear several advantages. However, few FC laboratories can rely on a long-standing practical experience, and the literature in support is still limited; as a result, the use of FC is generally restricted to the analysis of lymphomas with bone marrow or peripheral blood involvement. In this work, we applied machine learning to our database of 1465 B-NHL samples from different sources, building four artificial predictive systems which could classify B-NHL in up to nine of the most common clinico-pathological entities. Read More

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http://dx.doi.org/10.3390/cancers12061684DOI Listing

[Morphological and Immunohystochemical Characteristics of Aspirated Thrombi in Patients with ST-Elevation Myocardial Infarction].

Ter Arkh 2019 Sep 15;91(9):21-25. Epub 2019 Sep 15.

Yerevan State Medical University.

The aim of study was to evaluate the morphological and immunohystochemical characteristics of aspirated thrombi in patients with ST-elevation myocardial infarction undergoing precutaneous coronary intervention to find the possible platelet activation and inflammatory cell involvement in coronary thrombi.

Materials And Methods: Thrombi collected from 67 patients with ST-elevation myocardial infarction were studied. Macroscopic and immunohystochemical analyses were performed to reveal the cellular components of thrombi. Read More

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http://dx.doi.org/10.26442/00403660.2019.09.000199DOI Listing
September 2019

Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.

Endocr Regul 2020 Apr;54(2):77-84

Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Objective: The goal of this study was to reveal the impact of four types of atypical antipsychotics including amisulpride (AMI), olanzapine (OLA), quetiapine (QUE), and aripiprazole (ARI), with different receptor-affinity profile and dissociation constant, on the activity of hypothalamic supraoptic nucleus (SON) vasopressinergic and oxytocinergic neurons.

Methods: Male Sprague Dawley rats received a single injection of vehicle (VEH) (0.1 ml/100g), AMI (20 mg/kg), OLA (5 mg/kg), QUE (15 mg/kg/) or ARI (10 mg/kg). Read More

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http://dx.doi.org/10.2478/enr-2020-0010DOI Listing

Effects of Compound Centella on Oxidative Stress and Keap1-Nrf2-ARE Pathway Expression in Diabetic Kidney Disease Rats.

Evid Based Complement Alternat Med 2020 30;2020:9817932. Epub 2020 May 30.

Department of Nephrology, Key Laboratory of Zhejiang Province, Management of Kidney Disease, Hangzhou Hospital of Traditional Chinese Medicine, No. 453 Stadium Road, Hangzhou 310007, China.

The formula of Compound Centella mainly contains 3 traditional Chinese herbs: (L.) Urb. (JiXueCao), Fish. Read More

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http://dx.doi.org/10.1155/2020/9817932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277064PMC

The Prognostic Value of PARP Expression in High-Grade Epithelial Ovarian Cancer.

Pathol Oncol Res 2020 Jun 27. Epub 2020 Jun 27.

Institute of Obstetrics and Gynaecology, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.

In an attempt to clarify the prognostic relevance of poly (ADP-ribose) polymerase (PARP) expression, we analysed the clinical data of 86 high-grade epithelial ovarian cancer (EOC) cases in which PARP immunohistochemistry results were available. Immunostaining to highlight PARP protein expression was performed using a Leica Bond MAX Immunostainer (Leica Microsystems, Wetzlar, Germany). We applied a rabbit polyclonal anti-PARP antibody (ab6079 330, Abcam, Cambridge, UK) for the specific reaction. Read More

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http://dx.doi.org/10.1007/s12253-020-00856-6DOI Listing

Protein Sumoylation with SUMO1 Promoted by Pin1 in Glioma Stem Cells Augments Glioblastoma Malignancy.

Neuro Oncol 2020 Jun 27. Epub 2020 Jun 27.

Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.

Background: The tumorigenic potential of glioma stem cells (GSCs) is associated with multiple reversible molecular alternations, but the role of post-translational protein sumoylation in GSCs has not been elucidated. The development of GSC-targeting drugs relies on the discovery of GSC-preferential molecular modifications and the relevant signaling pathways. In this work, we investigated the protein sumoylation status, the major sumoylated substrate and the key regulatory enzyme in GSCs to explore the therapeutic potential of disrupting protein sumoylation for glioblastoma (GBM) treatment. Read More

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http://dx.doi.org/10.1093/neuonc/noaa150DOI Listing

VNUT/SLC17A9, a vesicular nucleotide transporter, regulates osteoblast differentiation.

FEBS Open Bio 2020 Jun 26. Epub 2020 Jun 26.

Division of Orofacial Functions and Orthodontics, Department of Health Improvement, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu-shi, 803-8580, Japan, Kitakyushu.

Osteoblasts release ATP out of the cell following mechanical stress. Although it is well established that extracellular ATP affects bone metabolism via P2 receptors (such as P2X7R and P2Y2R), the mechanism of ATP release from osteoblasts remains unknown. Recently, a vesicular nucleotide transporter (VNUT, SLC17A9) that preserves ATP in vesicles has been identified. Read More

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http://dx.doi.org/10.1002/2211-5463.12918DOI Listing

Identification of novel Lynch syndrome mutations in Chinese patients with endometriod endometrial cancer.

Cancer Biol Med 2020 May;17(2):458-467

Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China.

Lynch syndrome (LS) predisposes patients to early onset endometrioid endometrial cancer (EEC). However, little is known about LS-related EEC in the Chinese population. The aim of this study was to investigate the prevalence of LS and to identify the specific variants of LS in Chinese patients with EEC. Read More

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http://dx.doi.org/10.20892/j.issn.2095-3941.2019.0295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309470PMC

Knockdown of PLOD3 suppresses the malignant progression of renal cell carcinoma via reducing TWIST1 expression.

Mol Cell Probes 2020 Jun 22:101608. Epub 2020 Jun 22.

Department of Urology Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, 050000, China. Electronic address:

Procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD3), also known as lysyl hydroxylase 3 (LH3) has been demonstrated to be overexpressed in several kinds of cancers and facilitate cell migration. Currently, we aimed to reveal the role of PLOD3 in renal cell carcinoma (RCC) progression, and explore whether TWIST1 (Twist family bHLH transcription factor 1) is involved in this process. Fifty-eight paired RCC tissues and normal tissues were collected and subjected to qPCR and immunohistochemistry (IHC) technology to detect the expression levels of PLOD3. Read More

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http://dx.doi.org/10.1016/j.mcp.2020.101608DOI Listing

The presence of neutrophils causes RANKL expression in periodontal tissue, giving rise to osteoclast formation.

J Periodontal Res 2020 Jun 25. Epub 2020 Jun 25.

Department of Oral Biology, Yonsei University College of Dentistry, Seoul, South Korea.

Backgrounds And Objective: Increased neutrophil infiltration and osteoclast formation are key characteristics of periodontitis. The effect of these neutrophils on osteoclast formation in periodontitis remains unclear. Therefore, we investigated the effects of neutrophils on osteoclast formation in a neutrophil-deficient mouse model of periodontitis. Read More

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http://dx.doi.org/10.1111/jre.12779DOI Listing

Immunohistochemical expression of interleukin-17 and hormonal receptors in benign and malignant breast lesions.

BMC Res Notes 2020 Jun 23;13(1):300. Epub 2020 Jun 23.

Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

Objectives: IL17 is a critical pro-inflammatory cytokine that is involved in inflammation, multidrug resistance and growth persistence pathways in cancer. This study is aiming at studying the expression of IL17 and hormonal receptors expression in benign and malignant breast lesions using immunohistochemical staining methods.

Results: A total of 137 cases of breast lesions were studied, 97 (70. Read More

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http://dx.doi.org/10.1186/s13104-020-05146-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310502PMC

Investigation of the protective role of chrysin within the framework of oxidative and inflammatory markers in experimental testicular ischaemia/reperfusion injury in rats.

Andrologia 2020 Jun 22:e13714. Epub 2020 Jun 22.

Vocational School of Health Services, Van Yuzuncu Yıl University, Van, Turkey.

This study was performed to evaluate the effect of chrysin on testicular torsion and detorsion damage in rats in terms of biochemistry, histopathology and immunohistochemistry. The study was performed on Wistar albino rats between 250 g and 300 g. A total of 40 rats were used. Read More

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http://dx.doi.org/10.1111/and.13714DOI Listing

Prognostic potential of automated Ki67 evaluation in breast cancer: different hot spot definitions versus true global score.

Breast Cancer Res Treat 2020 Jun 22. Epub 2020 Jun 22.

Department of Oncology and Pathology, CCK, Karolinska Institutet, R8:04, 17176, Stockholm, Sweden.

Purpose: The proliferation-associated biomarker Ki67 has potential utility in breast cancer, including aiding decisions based on prognosis, but has unacceptable inter- and intralaboratory variability. The aim of this study was to compare the prognostic potential for Ki67 hot spot scoring and global scoring using different digital image analysis (DIA) platforms.

Methods: An ER+/HER2- breast cancer cohort (n = 139) with whole slide images of sequential sections stained for hematoxylin-eosin, pancytokeratin and Ki67, was analyzed using two DIA platforms. Read More

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http://dx.doi.org/10.1007/s10549-020-05752-wDOI Listing