7,296 results match your criteria Applied biochemistry and biotechnology[Journal]


Characterization of Glucokinase Catalysis from a Pseudo-Dimeric View.

Appl Biochem Biotechnol 2019 Apr 22. Epub 2019 Apr 22.

Department of Paper and Bioprocess Engineering, State University of New York College of Environmental Science and Forestry, Syracuse, NY, 13210, USA.

Glucose phosphorylation by glucokinase exhibits a sigmoidal dependency on substrate concentration regardless of its simple structure. Dimorph mechanism suggested the existence of two enzymatic states with different catalytic properties, which has been shown to be plausible by structural analysis. However, the dimorph mechanism gives rise to a complicated or non-explicit non-closed mathematical form. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02998-zDOI Listing

Accumulation of Lipids and Triglycerides in Isochrysis galbana Under Nutrient Stress.

Appl Biochem Biotechnol 2019 Apr 22. Epub 2019 Apr 22.

Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.

This study examines the relationship between the algal biomass as well as lipid and triglyceride contents in Isochrysis galbana and variation in nutrient culture conditions such as phosphorus concentration and iron concentration. From the selected experiments, a clear algal growth drop expressed by number of cells, optical density and dried weight, as well strongly participated in the lipid and triglyceride cumulation, which show a positive significant effect on lipid productivity and triglyceride productivity. In the first experiment of this study, the phosphorus concentration in the medium was reduced to 75%, 50%, and 25% and complete absence (0%) of the original concentration in Walne's medium, whereas the iron concentration was kept constant in the medium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02997-0DOI Listing

Interrelations of Apoptotic and Cellular Senescence Genes Methylation in Inflammatory Bowel Disease Subtypes and Colorectal Carcinoma in Egyptians Patients.

Appl Biochem Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Students at Faculty of Medicine, Faculty of Pharmacy, Assiut University, Assiut, Egypt.

Ras-related domain family member 1 transcript variant A (RASSF1A) controls apoptosis and cell proliferation while p14/ARF gene has a regulatory role in cellular senescence. Failure of apoptosis and cellular senescence occurs during inflammatory bowel disease (IBD) and colorectal cancer (CRC). To reveal the role of peripheral leukocyte promoter methylation of RASSF1A and p14/ARF in the pathogenesis of IBD subtypes and CRC we investigated the methylation state of the two genes by methylation-specific polymerase chain reaction (MSP-PCR) in 60 CRC patients, 60 patients with IBD; 27 with ulcerative colitis and 33 had Crohn's disease and also in 30 healthy subjects. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-03017-x
Publisher Site
http://dx.doi.org/10.1007/s12010-019-03017-xDOI Listing
April 2019
3 Reads

Anti-diabetic Properties of Calcium Channel Blockers: Inhibition Effects on Aldose Reductase Enzyme Activity.

Appl Biochem Biotechnol 2019 Apr 13. Epub 2019 Apr 13.

Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Turkey.

Aldose reductase (AR) belongs to NADPH-dependent oxidoreductases and converts glucose to sorbitol in the polyol pathway. AR inhibition is essential to prevent diabetic complications. Here, AR was purified from sheep kidney using simple methods and determined the interactions between some calcium channel blockers and the enzyme. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03009-xDOI Listing

Amino Acids as Additives against Amorphous Aggregation: In Vitro and In Silico Study on Human Lysozyme.

Appl Biochem Biotechnol 2019 Apr 13. Epub 2019 Apr 13.

Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Jalal-al-Ahmad street, Chamran Highway, Tehran, 1411713137, Iran.

The effect of 16 amino acids (AA) with various physicochemical properties was investigated on human lysozyme (HL) heat-induced amorphous aggregation. UV-Visible spectrophotometry was used to monitor the kinetics of aggregation in the absence and presence of AA, and transmission electron microscopy (TEM) images were taken from the aggregates. To conduct in silico experiments, Autodock vina was used for docking of AA into protein (via YASARA interface), and FTmap information was checked for an insight onto putative binding sites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03010-4DOI Listing
April 2019
2 Reads

Mesenchymal Stem Cells: a Promising Therapeutic Tool for Acute Kidney Injury.

Appl Biochem Biotechnol 2019 Apr 12. Epub 2019 Apr 12.

Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Acute kidney injury (AKI) is a rapid loss of renal function. It has high mortality rates. Still, renal replacement therapy is considered the best solution for recovering AKI. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02995-2DOI Listing
April 2019
1 Read

Extracellular Expression of L-Aspartate-α-Decarboxylase from Bacillus tequilensis and Its Application in the Biosynthesis of β-Alanine.

Appl Biochem Biotechnol 2019 Apr 11. Epub 2019 Apr 11.

School of Life Sciences, Ludong University, Yantai, 264025, China.

L-aspartate-α-decarboxylase was extracellularly expressed to enhance its production for β-alanine biosynthesis. L-aspartate-α-decarboxylase and cutinase were coexpressed in Escherichia coli; more than 40% of the L-aspartate-α-decarboxylase was secreted into the medium. Selection of best conditions among tested variables enhanced L-aspartate-α-decarboxylase production by the recombinant strain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03013-1DOI Listing

Gaseous Formaldehyde Degrading by Methylobacterium sp. XJLW.

Appl Biochem Biotechnol 2019 Apr 11. Epub 2019 Apr 11.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, People's Republic of China.

Formaldehyde is harmful to human beings. It is widely used in chemical industry, medicine, and agriculture and is frequently discharged into the sewage. Microbial metabolism of formaldehyde has attracted increasing attention for its potential application in formaldehyde removal, especially for indoor gaseous formaldehyde degradation. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-03001-5
Publisher Site
http://dx.doi.org/10.1007/s12010-019-03001-5DOI Listing
April 2019
9 Reads

Extraction of Flavonoids from the Saccharification of Rice Straw Is an Integrated Process for Straw Utilization.

Appl Biochem Biotechnol 2019 Apr 11. Epub 2019 Apr 11.

Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.

To date, bioethanol is not economically competitive. One strategy to overcome this limitation is co-producing ethanol and high value-added products as an integrated process. The results of this study demonstrated that flavonoids could be extracted from rice straw, and the flavonoids apigenin and kaempferol were detected by HPLC. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-03002-4
Publisher Site
http://dx.doi.org/10.1007/s12010-019-03002-4DOI Listing
April 2019
6 Reads

Utilization of a Silicone Rubber Membrane for Passive Oxygen Supply in a Microbial Fuel Cell Treating Carbon and Nitrogen from Synthetic Coke-Oven Wastewater.

Appl Biochem Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

Department of Civil, Architectural and Environmental Engineering, Ibaraki University, Hitachi, Ibaraki, 316-8511, Japan.

This study firstly introduced a silicone rubber membrane (SRM) into microbial fuel cell (MFC) for passive oxygen supply to simultaneously remove phenol and nitrogen from synthetic coke-oven wastewater diluted with seawater. Passive oxygen transport with biofilm on the membrane was improved by ~ 18-fold in comparison with the one without a biofilm. In addition, although the oxygen supply was passive, nitrification accounted for 34% of those aeration conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02994-3DOI Listing

Response of Propionate-Degrading Methanogenic Microbial Communities to Inhibitory Conditions.

Appl Biochem Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

College of Architecture and Environment, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu, 610065, Sichuan, China.

Propionate is a crucial intermediate during methane fermentation. Investigating the effects of different kinds of inhibitors on the propionate-degrading microbial community is necessary to develop countermeasures for improving process stability. In the present study, under inhibitory conditions (acetate, propionate, sulfide, and ammonium addition), the dynamic changes of the propionate-degrading microbial community from a mesophilic chemostat fed with propionate as the sole carbon source were investigated using high-throughput sequencing of 16S rRNA. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-03005-1
Publisher Site
http://dx.doi.org/10.1007/s12010-019-03005-1DOI Listing
April 2019
3 Reads

Synthesis, Crystal Structure, Hirshfeld Surface Analysis, DFT, and DNA-Binding Studies of (E)-2-(3-Hydroxy-4-Methoxybenzylidene)Hydrazinecarbothioamide.

Appl Biochem Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

Department Chemie, Fakultätfür Naturwissenschaften, Universität Paderborn, Warburgerstrasse 100, 33098, Paderborn, Germany.

(E)-2-(3-Hydroxy-4-methoxybenzylidene)hydrazinecarbothioamide 3 was synthesized by reacting thiosemicarbazide with 2-hydorxy-3-methoxybenzaldehyde in dry ethanol. The structure was elucidated by spectroscopic (FT-IR, H NMR, and C NMR) and single crystal X-ray diffraction techniques. A detailed analysis of the intermolecular interactions has been performed based on the Hirshfeld surfaces and their associated two-dimensional fingerprint plots. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03008-yDOI Listing
April 2019
1.735 Impact Factor

Improved Carotenoid Productivity and COD Removal Efficiency by Co-culture of Rhodotorula glutinis and Chlorella vulgaris Using Starch Wastewaters as Raw Material.

Appl Biochem Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.

Utilization of low-cost raw materials for the bio-based chemical production, such as carotenoids, by the co-culture of Rhodotorula glutinis and Chlorella vulgaris has recently become an attractive option. In this study, the primary nutrients of starch wastewater were analyzed, which were used for carotenoid production by the co-culture strategy in a 5-L fermenter around 4000 Lux light intensity. Synergistic effect of gas utilization revealed that the two species could build up the beneficial balance on mutualism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03016-yDOI Listing

Lactic Acid Production from a Whole Slurry of Acid-Pretreated Spent Coffee Grounds by Engineered Saccharomyces cerevisiae.

Appl Biochem Biotechnol 2019 Apr 10. Epub 2019 Apr 10.

School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, South Korea.

Spent coffee grounds (SCG) generated after coffee extraction are the main byproduct of the coffee industry. Valorization of the SCG has been increasingly focused following considerable attention in coffee consumption. Lactic acid bacteria fermentation is the primary source of generation of lactic acid, a monomer of polylactic acid that has various industrial applications; however, because of the low tolerance of lactic acid bacteria to toxic compounds, it is necessary to apply Saccharomyces cerevisiae to produce lactic acid whose tolerance to toxic compounds is higher. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-03000-6
Publisher Site
http://dx.doi.org/10.1007/s12010-019-03000-6DOI Listing
April 2019
3 Reads

Post-treatment of Fungal Biomass to Enhance Pigment Production.

Appl Biochem Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Swedish Centre for Resource Recovery, University of Borås, Allégatan 1, 503 32, Borås, Sweden.

A new post-treatment method of fungal biomass after fermentation is revealed. The post-treatment strategy was utilized to produce pigments as an additional valuable metabolite. Post-treatment included incubation at 95% relative humidity where the effects of harvesting time, light, and temperature were studied. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-02961-y
Publisher Site
http://dx.doi.org/10.1007/s12010-019-02961-yDOI Listing
April 2019
1 Read

In Vitro Thermal and Ethanol Adaptations to Improve Vinegar Fermentation at High Temperature of Komagataeibacter oboediens MSKU 3.

Appl Biochem Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok, 10900, Thailand.

High temperature and high ethanol concentrations obviously affect vinegar fermentation. The thermotolerant and ethanol-resistant strains are expected to become one of the technologies for effective vinegar fermentation. This study aimed to further improve thermotolerant Komagataeibacter oboediens MSKU 3 through thermal and ethanol adaptations for acetic acid fermentation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03003-3DOI Listing
April 2019
1 Read

Bio-detoxification Bacteria Isolated from Dye-Polluted Soils Promote Lactic Acid Production from Ammonia Pretreated Corn Stover.

Appl Biochem Biotechnol 2019 Mar 25. Epub 2019 Mar 25.

State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.

Agro-stovers are the most abundant substrates for producing lactic acid, which has great potential application in the production of biodegradable and biocompatible polylactic acid polymers. However, chemical pretreatments on agro-stovers generate inhibitors that repress the subsequent lactic acid fermentation. In this study, three bacterial strains (Enterococcus faecalis B101, Acinetobacter calcoaceticus C1, and Pseudomonas aeruginosa CS) isolated from dye-polluted soils could utilize phenolic inhibitor mimics (vanillin, 4- hydroxybenzaldehyde, or syringaldehyde) from alkaline pretreated corn stovers as a sole carbon source. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02993-4DOI Listing
March 2019
2 Reads

Evaluation of the Potential Phosphorylation Effect on Isocitrate Dehydrogenases from Saccharomyces cerevisiae and Yarrowia lipolytica.

Appl Biochem Biotechnol 2019 Apr 21;187(4):1131-1142. Epub 2019 Mar 21.

Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, No.1 Beijing East Road, Wuhu, 241000, Anhui, China.

Escherichia coli isocitrate dehydrogenase (IDH) is regulated by reversible phosphorylation on Ser113. Latest phosphoproteomic studies revealed that eukaryotic IDHs can also be phosphorylated on the analogous Ser site. So as to understand the possible phosphorylation mechanism, the equivalent Ser of NADP-IDHs from yeast Saccharomyces cerevisiae (ScIDH) and Yarrowia lipolytica(YlIDH) were investigated by site-directed mutagenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02974-7DOI Listing

Overexpression of the DOF-Type Transcription Factor Enhances Lipid Synthesis in Chlorella vulgaris.

Appl Biochem Biotechnol 2019 Mar 16. Epub 2019 Mar 16.

Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.

In the present study, DNA binding with one finger (DOF)-type transcription factors were screened from the Chlorella vulgaris genome database. The candidate DOF transcription factor was endogenously overexpressed in C. vulgaris to improve neutral lipid production. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02990-7DOI Listing
March 2019
1 Read

Isolation of Bisphenol A-Tolerating/degrading Shewanella haliotis Strain MH137742 from an Estuarine Environment.

Appl Biochem Biotechnol 2019 Mar 14. Epub 2019 Mar 14.

Environmental Research and Education Center, CEPEMA-POLI-USP, University of São Paulo, Conego Domenico Rangoni Rd, 270 km, Cubatão, São Paulo, Brazil.

The human exposure to bisphenol A (BPA) occurs frequently. Once, this compound was one of the highest volume chemicals produced worldwide and used as a plasticizer in many products. However, even at low concentration, it can cause severe damage to the endocrine system because of its endocrine disruptor activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02989-0DOI Listing
March 2019
6 Reads

Optimization of Cellulase and Xylanase Productions by Streptomyces thermocoprophilus Strain TC13W Using Oil Palm Empty Fruit Bunch and Tuna Condensate as Substrates.

Appl Biochem Biotechnol 2019 Mar 14. Epub 2019 Mar 14.

Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90110, Thailand.

The modified medium composed of the alkaline-pretreated oil palm empty fruit bunch (APEFB) and tuna condensate powder was used for cellulase and xylanase productions by Streptomyces thermocoprophilus strain TC13W. The APEFB contained 74.46% (w/w) cellulose, 15. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02986-3DOI Listing
March 2019
1 Read

Production, Partial Purification, and Biochemical Characterization of a Thermotolerant Alkaline Metallo-protease from Staphylococcus sciuri.

Appl Biochem Biotechnol 2019 Mar 14. Epub 2019 Mar 14.

Chemistry Department, Biochemistry Branch, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Protease-producing Staphylococcus sciuri was isolated from poultry soil samples and culture conditions for protease production were optimized. The isolated protease showed a maximum activity of 235.1 U/ml. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02983-6DOI Listing

Two-Step Pretreatment of Corn Stover Silage Using Non-ionic Surfactant and Ferric Nitrate for Enhancing Sugar Recovery and Enzymatic Digestibility of Cellulose.

Appl Biochem Biotechnol 2019 Mar 13. Epub 2019 Mar 13.

Tianjin Key Laboratory of Clean Energy and Pollution Control, School of Energy and Environmental Engineering, Hebei University of Technology, Tianjin, 300401, China.

Corn stover silage (CSS) is regarded as a promising feedstock for bioethanol production. The two-step pretreatment using a sequential non-ionic surfactant and ferric nitrate pretreatment was investigated for improving the enzymatic hydrolysis of CSS. The first-step pretreatment using non-ionic surfactant (Tween-80, 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02988-1DOI Listing

Use of an (Hemi) Cellulolytic Enzymatic Extract Produced by Aspergilli Species Consortium in the Saccharification of Biomass Sorghum.

Appl Biochem Biotechnol 2019 Mar 13. Epub 2019 Mar 13.

Agricultural Sciences Institute (ICIAG-UFU), Uberlândia Federal University, Uberlândia, Minas Gerais, 38405-320, Brazil.

This study evaluated the production of lignocellulose-degrading enzymes by solid-state fermentation (SSF) using a microbial consortium of Aspergillus fumigatus SCBM6 and A. niger SCBM1 (AFN extract). The fungal strains were cultivated in sugarcane bagasse (SCB) and wheat bran (WB) as lignocellulosic substrates for 7 days at 30 °C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02991-6DOI Listing
March 2019
1 Read

Inhibitory Effects of Vanadium-Binding Proteins Purified from the Sea Squirt Halocynthia roretzi on Adipogenesis in 3T3-L1 Adipocytes.

Appl Biochem Biotechnol 2019 Mar 13. Epub 2019 Mar 13.

Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, 25457, Republic of Korea.

The inhibitory effects of vanadium-binding proteins (VBPs) from the blood plasma and the intestine of sea squirt on adipogenesis in 3T3-L1 adipocytes were examined. 3T3L-1 cells treated with VBP decreased markedly the lipid content in maturing pre-adipocytes in a dose-dependent manner, whereas VBP did not show significant effects on lipid accumulation. Both VBPs did not have significant effect on cell viability. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02982-7DOI Listing

Establishment of CRISPR/Cas9-Mediated Knock-in System for Porcine Cells with High Efficiency.

Appl Biochem Biotechnol 2019 Mar 12. Epub 2019 Mar 12.

College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Since the birth of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9, the new genome engineering technology has become a hot topic in the scientific community. However, for swine, the system of pig cells' homology directed repair (HDR) is generally unstable and costly. Here, we aim to make knock-in of porcine cells more realizable. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02984-5DOI Listing
March 2019
1.735 Impact Factor

Fluoranthene Biodegradation by Serratia sp. AC-11 Immobilized into Chitosan Beads.

Appl Biochem Biotechnol 2019 Mar 11. Epub 2019 Mar 11.

Laboratory of Natural Organic Matter, Department of Chemistry, Federal University of Sergipe, Av. Marechal Rondon, Jardim Rosa Elze, Sao Cristovao, SE, 49100-000, Brazil.

The intensive production of polycyclic aromatic hydrocarbons by anthropogenic activities is a serious environmental problem. Therefore, new bioremediation methods are required to avoid widespread contamination. In this work, Serratia sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02980-9DOI Listing

Characterization of a Novel Neoagarobiose-Producing GH42 β-Agarase, AgaJ10, from Gayadomonas joobiniege G7.

Appl Biochem Biotechnol 2019 Mar 11. Epub 2019 Mar 11.

Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, 449-728, Republic of Korea.

Gayadomonas joobiniege G7 is an agar-degrading bacterium, which produces various agarases that have been biochemically characterized recently. In this study, we biochemically characterized a new β-agarase AgaJ10 belonging to the glycoside hydrolase (GH) 42 family from G. joobiniege G7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02992-5DOI Listing

Major Phytoconstituents of Prunus cerasoides Responsible for Antimicrobial and Antibiofilm Potential Against Some Reference Strains of Pathogenic Bacteria and Clinical Isolates of MRSA.

Appl Biochem Biotechnol 2019 Mar 11. Epub 2019 Mar 11.

Microbial Technology Laboratory, Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, India.

Prunus cerasoides is a traditionally well known for human health in various ways and particularly its bark is reported to possess high therapeutic applications in wound healing, foot and mouth disease, and indigestion etc. But there is scanty literature available on its systematic studies and phytoconstituents responsible for antimicrobial activity so the work is proposed. The main aim of this study is to reveal the phytoconstituents responsible for antimicrobial and antibiofilm action to demonstrate the effectiveness of such compounds by extrapolating the data using clinical isolates of pathogenic bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02985-4DOI Listing

Improvement of Methane Production from Sugar Beet Wastes Using TiO and FeO Nanoparticles and Chitosan Micropowder Additives.

Appl Biochem Biotechnol 2019 Mar 11. Epub 2019 Mar 11.

Department of Chemical Engineering, Quchan University of Technology, Quchan, 9477167335, Iran.

An experimental study was performed to measure biogas production from sugar beet waste, which is, in fact, the chopped parts of the sugar beet not going through the sugar extraction process, at different additive concentrations. Medium molecular weight chitosan in microsize and TiO and FeO nanoparticles were added to ten experimental reactors to investigate their effect on the anaerobic digestion process. Three different concentrations of 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02987-2DOI Listing

Assessing Activity of Antimicrobial Agents and Screening Antibiotic-Resistant Bacteria Through DREAM Assay.

Appl Biochem Biotechnol 2019 Mar 2. Epub 2019 Mar 2.

Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, Puttaparthi, Andhra Pradesh, 515134, India.

Antibiotics have assumed importance in the scene of medicine due to their activity against bacterial infections. During the course of evolution, bacteria have been developing routes to overcome damaging effects of antibiotics. Antibiotic resistance is an intriguing development that has taken the world of anti-microbial therapy by storm. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02981-8DOI Listing

Development of Voltammetric Glucose-6-phosphate Biosensors Based on the Immobilization of Glucose-6-phosphate Dehydrogenase on Polypyrrole- and Chitosan-Coated FeO Nanoparticles/Polypyrrole Nanocomposite Films.

Appl Biochem Biotechnol 2019 Feb 28. Epub 2019 Feb 28.

Science Faculty, Department of Molecular Biology and Genetics, Istanbul University, Fatih, 34134, Istanbul, Turkey.

Polypyrrole (PPy) and PPy-containing chitosan-coated FeO have been electrochemically polymerized on pencil graphite electrodes (PGEs). After the resulting electrodes were characterized by SEM-EDS analysis, glucose-6-phosphate dehydrogenase (G6PD) was immobilized onto these electrodes via glutaraldehyde. The biosensors prepared for the chronopotentiometric detection of glucose-6-phosphate (G6P) at 0. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-02979-2
Publisher Site
http://dx.doi.org/10.1007/s12010-019-02979-2DOI Listing
February 2019
6 Reads

Direct Write Assembly of Graphene/Poly(ε-Caprolactone) Composite Scaffolds and Evaluation of Their Biological Performance Using Mouse Bone Marrow Mesenchymal Stem Cells.

Appl Biochem Biotechnol 2019 Feb 27. Epub 2019 Feb 27.

Faculty of Engineering, Department of Metallurgical and Materials Engineering, Manisa Celal Bayar University, 45140, Yunusemre, Manisa, Turkey.

Scaffold and mesenchymal stem cell-based cartilage tissue engineering offers a favorable way for the repair and regeneration of injured cartilage. In this study, poly (ε-caprolactone) PCL scaffolds with grid-like structure having periodic lattice was manufactured by robocasting method in the presence of graphene nanoplatelets for cartilage tissue engineering applications. For this purpose, a PCL solution (20 wt%) containing pristine graphene nanopowders in the form of platelets was prepared as printing ink and it was dispensed through a nozzle at room temperature to an ethanol bath at 4 °C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02976-5DOI Listing
February 2019
5 Reads

Characterization of Two Polyketide Synthases Involved in Sorbicillinoid Biosynthesis by Acremonium chrysogenum Using the CRISPR/Cas9 System.

Authors:
Guozhi Chen Ju Chu

Appl Biochem Biotechnol 2019 Feb 27. Epub 2019 Feb 27.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.

Acremonium chrysogenum is an important fungal strain used for cephalosporin C production. Many efforts have been made to develop versatile genome-editing tools to better understand the mechanism of A. chrysogenum. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02960-zDOI Listing
February 2019

Force Sensing in Nanoscale: Integration of Virtual Spring for Sensing the Interactions of β-Amyloid Grabbed by Prefoldin.

Appl Biochem Biotechnol 2019 Feb 26. Epub 2019 Feb 26.

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran, P.O. Box 1435116471, Tehran, Iran.

Analysis of atomic forces in molecular scale including the reciprocal internal force directions, quantity, and Young's modulus, and more detailed analysis via diagrams, is one of the most current research topics. This research aims to analyze the bilateral forces applied between a nano-actuator and a cargo from a new perspective. While virtual springs have been used as a tool to convey forces in the previous steered molecular dynamics simulations, this study uses the spring as a sensor for measuring the internal forces. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02975-6DOI Listing
February 2019

Author Correction: Identification of a Novel Feruloyl Esterase by Functional Screening of a Soil Metagenomic Library.

Appl Biochem Biotechnol 2019 04;187(4):1582-1583

Key Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

The original version of this article unfortunately contained a mistake in the image and caption of Fig. 6. The corrected version of the image and caption is shown here. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02978-3DOI Listing
April 2019
2 Reads

Production of a Potentially Synbiotic Pomegranate Beverage by Fermentation with Lactobacillus plantarum ATCC 14917 Adsorbed on a Prebiotic Carrier.

Appl Biochem Biotechnol 2019 Feb 21. Epub 2019 Feb 21.

Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agriculture Development, Democritus University of Thrace, 68200, Orestiada, Greece.

A probiotic biocatalyst was prepared through Lactobacillus plantarum ATCC 14917 immobilization on a prebiotic carrier (delignified wheat bran) and was used for fermentations of pomegranate juice. Initially, pomegranate juice was fermented for 24 h and then was stored for 28 days at 4 °C. The obtained results regarding sugar and organic acid analysis revealed that the probiotic biocatalyst was effective. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02977-4DOI Listing
February 2019

Molecular Cloning and Characterization of a Novel Cold-Adapted Alkaline 1,3-α-3,6-Anhydro-L-galactosidase, Ahg558, from Gayadomonas joobiniege G7.

Appl Biochem Biotechnol 2019 Feb 21. Epub 2019 Feb 21.

Department of Bioscience and Bioinformatics, Myongji-Ro 116, Yongin, Gyeoggido, 17058, South Korea.

Agar, a major polysaccharide of red algal cells, is degraded by β-agarases into neoagarobiose, which is further hydrolyzed into the monomers, D-galactose and 3,6-anhydro-L-galactose, by 1,3-α-3,6-anhydro-L-galactosidases including α-1,3-L-neoagarooligasaccharide hydrolase (α-NAOSH). A novel cold-adapted alkaline α-NAOSH, Ahg558, consisting of 359 amino acids (40.8 kDa) was identified from Gayadomonas joobiniege G7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02963-wDOI Listing
February 2019

Prediction of Lignin Contents from Infrared Spectroscopy: Chemical Digestion and Lignin/Biomass Ratios of Cryptomeria japonica.

Appl Biochem Biotechnol 2019 Feb 20. Epub 2019 Feb 20.

College of Wood Science and Technology, Nanjing Forestry University, Nanjing, China.

A method for the high-throughput analysis of the relative lignin contents of Cryptomeria japonica samples over a wide concentration range (3-73%), independent of the type of chemical pretreatment, was developed by using Fourier transform infrared spectroscopy. First, the assignments of the infrared absorbance related to lignin were reviewed. Then, various chemical treatments, including alkaline, acid, and hydrothermal processes, and a sodium chlorite oxidation treatment, were performed to prepare samples containing a wide range of different lignin contents. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02965-8DOI Listing
February 2019

Effects of 2-(2-Chlorophenyl)ethylbiguanide on ERAD Component Expression in HT-29 Cells Under a Serum- and Glucose-Deprived Condition.

Appl Biochem Biotechnol 2019 Feb 16. Epub 2019 Feb 16.

Laboratory of Pharmaceutical & Medicinal Chemistry, Gifu Pharmaceutical University, 1-25-4, Daigakunishi, Gifu, 501-1196, Japan.

We recently characterized the cytotoxic action of a novel phenformin derivative, 2-(2-chlorophenyl)ethylbiguanide (2-Cl-Phen), on HT-29 cells under a serum- and glucose-deprived condition and found that 2-Cl-Phen attenuated ATF4 and GRP78, typical downstream targets of the unfolded protein response (UPR), together with c-Myc protein expression in a transcriptional and posttranscriptional manner. In the current study, we focused on the expression of ER-associated protein degradation (ERAD) components after treatment with 2-Cl-Phen under a serum- and glucose-deprived condition. Among nine ER-localizing factors regulating protein quality control within the ER, the amounts of Herp, GRP78, GRP94, and OS9 proteins were significantly downregulated by treatment with 2-Cl-Phen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02969-4DOI Listing
February 2019

Sequences, Domain Architectures, and Biological Functions of the Serine/Threonine and Histidine Kinases in Synechocystis sp. PCC 6803.

Authors:
Wu Xu Yingchun Wang

Appl Biochem Biotechnol 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Rd., Beijing, 100101, China.

The cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis) is a photoautotrophic prokaryote with plant-like photosynthetic machineries which significantly contribute to global carbon fixation and atmospheric oxygen production. Because of the relatively short cell doubling time, small size of the genome, and the ease for genetic manipulation, Synechocystis is a popular model organism for studies including photosynthesis and biofuel production. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02971-wDOI Listing
February 2019

Purification and Physicochemical Characterization of a Novel Thermostable Xylanase Secreted by the Fungus Myceliophthora heterothallica F.2.1.4.

Appl Biochem Biotechnol 2019 Feb 14. Epub 2019 Feb 14.

São Paulo State University (Unesp), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, São Paulo, Brazil.

Xylanases are enzymes that act in the depolymerization of xylan and that can be used in the food industry, the paper industry, and for bioenergy, among other uses. In this context, particular emphasis is devoted to xylooligosaccharides (XOS) that act as prebiotics, which, under the action of probiotic microorganisms, are capable of positively modifying the intestinal microbiota. In this sense, searching for microbial xylanases stands out as a sustainable strategy for the production of prebiotics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02973-8DOI Listing
February 2019
3 Reads

Detoxification of Hydrolysates of the Red Seaweed Gelidium amansii for Improved Bioethanol Production.

Appl Biochem Biotechnol 2019 Feb 14. Epub 2019 Feb 14.

Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea.

In this study, bioethanol was produced from the seaweed Gelidium amansii as biomass through separate hydrolysis and fermentation (SHF) processes. The SHF processes examined in this study include thermal acid hydrolysis pretreatment, enzymatic saccharification, detoxification, and fermentation. Thermal acid hydrolysis pretreatment was conducted using HSO, with a slurry content of 8-16% and treatment time of 15-75 min. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02970-xDOI Listing
February 2019
12 Reads

A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro.

Appl Biochem Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey.

The aim of this study was to investigate the effect of medium harvested from septal cartilage cells on chondrogenic differentiation of adipose stem cells (hASCs) and to compare/contrast its properties to those of a commonly used standard medium formulation in terms of induction and maintenance of chondrogenic hASCs. Differentiation was carried out under three different conditions: septal cartilage medium-SCM, chondrogenic differentiation medium-CM, and 50:50 mixture of CM/SCM. Mesenchymal stem cells (MSCs) markers were determined by flow cytometry. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s12010-019-02964-9
Publisher Site
http://dx.doi.org/10.1007/s12010-019-02964-9DOI Listing
February 2019
6 Reads
1.735 Impact Factor

Preparation of an Electrically Conductive Graphene Oxide/Chitosan Scaffold for Cardiac Tissue Engineering.

Appl Biochem Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

School of Life Science and Medicine, Dalian University of Technology, 2 Dagong Road, Liaodongwan New District, Panjin, 124221, China.

Cardiac tissue engineering is of great importance for therapeutic and pharmaceutical applications. The scaffolds that can provide electrical conductivity and structural organization will be highly beneficial for cardiac tissue engineering. Here, we developed conductive scaffolds with electrical conductivity and porous structure composed of chitosan (CS) blending with graphene oxide (GO) for cardiac tissue engineering. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02967-6DOI Listing
February 2019
2 Reads
1.735 Impact Factor

Biological Modification of Montan Resin from Lignite by Bacillus benzoevorans.

Appl Biochem Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

The montan resin (MR) is a solid waste produced during the industrial process of refined montan wax from lignite, and usually disposed by landfill and incineration, which easily cause environmental pollution and resource waste. Based its physicochemical properties, our study attempted to modify MR by Bacillus benzoevorans to achieve ecological utilization of MR. As results, the weight loss rate of MR, expressed as modification degree, was found to increase with the increase of B. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02962-xDOI Listing
February 2019
1 Read
1.735 Impact Factor

Mixture of Macromolecular Crowding Agents Has a Non-additive Effect on the Stability of Proteins.

Appl Biochem Biotechnol 2019 Feb 9. Epub 2019 Feb 9.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India.

The folding and unfolding of proteins inside a cell take place in the presence of macromolecules of various shapes and sizes. Such crowded conditions can significantly affect folding, stability, and biophysical properties of proteins. Thus, to logically mimic the intracellular environment, the thermodynamic stability of two different proteins (lysozyme and α-lactalbumin) was investigated in the presence of mixtures of three crowding agents (ficoll 70, dextran 70, and dextran 40) at different pH values. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02972-9DOI Listing
February 2019

Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116.

Appl Biochem Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Department of Chemical Engineering, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, 97105-900, Brazil.

The performance of lipase NS 40116, a novel and promising soluble enzyme obtained from modified Thermomyces lanuginosus microorganism, was investigated in the production of biodiesel (fatty acid methyl esters-FAME) by hydroesterification. In order to investigate the potential of the biocatalyst in its soluble form, this work reports the effect of water content and enzyme load, as well as the recovery and reuse of the biocatalyst. A FAME yield of 94. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02966-7DOI Listing
February 2019
2 Reads

Magneto-Priming Improved Nutraceutical Potential and Antimicrobial Activity of Momordica charantia L. Without Affecting Nutritive Value.

Appl Biochem Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Department of Chemistry, Government College University, Faisalabad, 38000, Pakistan.

The need for some economic strategies for increased growth and nutraceuticals of medicinal plants is well acknowledged now. It was hypothesized that external magnetic field treatment (MFT) of seeds affecting internal magnet of cells may affect growth and metabolism. In this study, seeds were subjected to pre-sowing magnetic field (50 mT at 5 mm for 5 s). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02955-wDOI Listing
February 2019
3 Reads
1.735 Impact Factor

A Membrane-Bound Gluconate Dehydrogenase from 2-Keto-D-Gluconic Acid Industrial Producing Strain Pseudomonas plecoglossicida JUIM01: Purification, Characterization, and Gene Identification.

Appl Biochem Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.

The membrane-bound gluconate dehydrogenase (mGADH) is a critical enzyme for 2-keto-D-gluconic acid (2KGA) production in Pseudomonas plecoglossicida JUIM01. The purified native flavin adenine dinucleotide-dependent mGADH (FAD-mGADH) was consisted of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular mass of ~ 27, 65, and 47 kDa, respectively. The specific activity of FAD-mGADH was determined as 90. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-02951-0DOI Listing
February 2019