7,627 results match your criteria Applied biochemistry and biotechnology[Journal]


Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.

Appl Biochem Biotechnol 2020 Jun 2. Epub 2020 Jun 2.

Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.

Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide. The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68. Read More

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http://dx.doi.org/10.1007/s12010-020-03345-3DOI Listing

Variation of Microbial Diversity in Catastrophic Oil Spill Area in Marine Ecosystem and Hydrocarbon Degradation of UCMs (Unresolved Complex Mixtures) by Marine Indigenous Bacteria.

Appl Biochem Biotechnol 2020 May 23. Epub 2020 May 23.

Department of Earth and Environmental Sciences, National Chung Cheng University, 168 University Road, Ming-Shung, Chiayi County, 62102, Taiwan.

The study targeted an assessment of microbial diversity during oil spill in the marine ecosystem (Kaohsiung port, Taiwan) and screened dominant indigenous bacteria for oil degradation, as well as UCM weathering. DO was detected lower and TDS/conductivity was observed higher in oil-spilled area, compared to the control, where a significant correlation (R = 1; P < 0.0001) was noticed between DO and TDS. Read More

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http://dx.doi.org/10.1007/s12010-020-03335-5DOI Listing
May 2020
1.735 Impact Factor

Cloning, Characterization, and Structural Modeling of an Extremophilic Bacterial Lipase Isolated from Saline Habitats of the Thar Desert.

Appl Biochem Biotechnol 2020 May 18. Epub 2020 May 18.

Department of Microbiology, Maharaja Ganga Singh University, Bikaner, 334004, India.

Lipases have a characteristic folding pattern of α/β-hydrolase with mostly parallel β-sheets, flanked on both sides by α-helixes in the structure. The active site is formed by a catalytic triad (serine, aspartic/glutamic acid, and histidine), which is highly conserved. In this study, we have used an integrated experimental and computational approach to identify the extremophilic microbial lipases from the saline habitats of the Thar Desert of Rajasthan. Read More

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http://dx.doi.org/10.1007/s12010-020-03329-3DOI Listing

Dimorphic Mechanism on cAMP Mediated Signal Pathway in Mucor circinelloides.

Appl Biochem Biotechnol 2020 May 18. Epub 2020 May 18.

Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama, Japan.

Mucor circinelloides is a dimorphic fungus that is a non-pathogen strain belonging to zygomycetes. In this research, a part of hypothetical mechanism on yeast-like cell induction of M. circinelloides in CO atmosphere was reported from the viewpoint of gene expression. Read More

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http://dx.doi.org/10.1007/s12010-020-03342-6DOI Listing

Metagenomic Insights in Activated Biomass Treating Industrial Wastewater at Different DO Levels.

Appl Biochem Biotechnol 2020 May 16. Epub 2020 May 16.

Director's Research Cell, National Environmental Engineering Research Institute, (CSIR-NEERI), Nehru Marg, Nagpur, 440 020, India.

Dissolved oxygen (DO) is an imperative parameter of the activated sludge process (ASP) for wastewater bioremediation. The effect of DO on microbial communities and corresponding metabolic functions in wastewater bioremediation was investigated using next-generation analysis techniques in this study. Illumina-based whole genome sequencing was applied to analyze the composition of the microbial community along with their functional diversity in activated sludge systems operating at three different DO levels. Read More

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http://dx.doi.org/10.1007/s12010-020-03340-8DOI Listing
May 2020
1.735 Impact Factor

Effect of Decellularization Parameters on the Efficient Production of Kidney Bioscaffolds.

Appl Biochem Biotechnol 2020 May 16. Epub 2020 May 16.

Biology Department, De La Salle University, Manila, Philippines.

The most preferred decellularization technique in creating bioscaffolds for complex organs such as kidneys is through detergent perfusion. Detergents such as sodium dodecyl sulfate (SDS) flow to the kidneys to remove cells but using this technique alone requires long treatment times. Coupling this technique with sonication treatment decreases decellularization time but may cause damages in the microarchitecture of the kidney. Read More

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http://dx.doi.org/10.1007/s12010-020-03338-2DOI Listing

Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration.

Appl Biochem Biotechnol 2020 May 13. Epub 2020 May 13.

State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.

Formate dehydrogenases are critical tools for nicotinamide cofactor regeneration, but their limited catalytic efficiency (k/K) is a major drawback. A formate dehydrogenase from Burkholderia stabilis 15516 (BstFDH) was the first native NADP-dependent formate dehydrogenase reported and has the highest k/K toward NADP (k/K) compared with other FDHs that can utilize NADP as a hydrogen acceptor. However, the substrate and cofactor affinities of BstFDH are inferior to those of other FDHs, making its practical application difficult. Read More

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http://dx.doi.org/10.1007/s12010-020-03317-7DOI Listing
May 2020
1.735 Impact Factor

Differential Activation of Ferulic Acid Catabolic Pathways of Amycolatopsis sp. ATCC 39116 in Submerged and Surface Cultures.

Appl Biochem Biotechnol 2020 May 12. Epub 2020 May 12.

Centro de Investigación en Alimentación y Desarrollo, A.C. (Coordinación de Ciencia de los Alimentos), Carretera Gustavo Enrique Astiazarán Rosas 46, La Victoria, CP, 83304, Hermosillo, Sonora, Mexico.

Amycolatopsis sp. ATCC 39116 catabolizes ferulic acid by the non-oxidative deacetylation and β-oxidation pathways to produce vanillin and vanillic acid, respectively. In submerged culture, vanillin productivity decreased more than 8-fold, when ferulic, p-coumaric, and caffeic acids were employed in pre-cultures of the microorganism in order to activate the ferulic acid catabolic pathways, resulting in a carbon redistribution since vanillic acid and guaiacol productivities increased more than 5-fold compared with control. Read More

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http://dx.doi.org/10.1007/s12010-020-03336-4DOI Listing

Concentration of Docosahexaenoic and Eicosapentaenoic Acid from Cobia Liver Oil by Acetone Fractionation of Fatty Acid Salts.

Appl Biochem Biotechnol 2020 May 12. Epub 2020 May 12.

Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan.

The production of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) concentrate from cobia liver oil by acetone fractionation of fatty acid salts was investigated in this study. A three-level-three-factor Box-Behnken design was used to evaluate the effects of reaction time, amount of NaOH added, and acetone ratio on the responses (DHA and EPA content and recovery). The results showed that the amount of NaOH added was the most important factor in the process. Read More

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http://dx.doi.org/10.1007/s12010-020-03341-7DOI Listing

High Productivity Ethanol from Solid-State Fermentation of Steam-Exploded Corn Stover Using Zymomonas mobilis by N Periodic Pulsation Process Intensification.

Appl Biochem Biotechnol 2020 May 12. Epub 2020 May 12.

State Key Laboratory of Biochemical Engineering, Beijing Key Laboratory of Biomass Refining Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.

Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol fermentation. Z. Read More

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http://dx.doi.org/10.1007/s12010-020-03318-6DOI Listing

Biogas from Tannery Solid Waste Anaerobic Digestion Is Driven by the Association of the Bacterial Order Bacteroidales and Archaeal Family Methanosaetaceae.

Appl Biochem Biotechnol 2020 May 12. Epub 2020 May 12.

Laboratory for Leather and Environmental Studies - LACOURO, Chemical Engineering Department, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, Brazil.

The search for renewable energies has been one of the biggest challenges of the last decades. Sludge and solid wastes of many sources have been used to produce biogas of high calorific value. Thus, this work aimed to evaluate the biogas production of solid waste originating from a tannery that uses chromium salts as a tanning agent and to characterize the physicochemical parameters and microbial composition of the biogas-producing biomass. Read More

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http://dx.doi.org/10.1007/s12010-020-03326-6DOI Listing

Anoxic Microbial Community Robustness Under Variation of Hydraulic Retention Time and Availability of Endogenous Electron Donors.

Appl Biochem Biotechnol 2020 May 11. Epub 2020 May 11.

Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Av. Trabalhador São-carlense no. 400, São Carlos, SP, 13566-590, Brazil.

The ADNMED (Anaerobic Digestion, Nitrification, and Mixotrophic Endogenous Denitrification) system comprises a triple chamber configuration that was shown to provide high-quality effluent regarding carbon, nitrogen, and sulfide. Hydraulic retention time (HRT) was 7 h in the anaerobic and anoxic chambers, and 5 h in the aerobic chamber (stage A). Sewage was directly added to the anoxic chamber to provide extra organic electron donors for denitrification (stage B) to improve the nitrogen removal efficiency (stage A 47 ± 19%). Read More

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http://dx.doi.org/10.1007/s12010-020-03327-5DOI Listing

An Innovator Support Material for Tyrosinase Immobilization: Antimony-Doped Tin Oxide Thin Films (ATO-TF).

Appl Biochem Biotechnol 2020 May 11. Epub 2020 May 11.

Department of Electrical and Electronics Engineering, Faculty of Engineering, Iğdır University, 76000, Iğdır, Turkey.

Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectroscopy (FTIR). The optimum pH of the immobilized tyrosinase (tyrosinase-ATO-TF) was 6. Read More

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http://dx.doi.org/10.1007/s12010-020-03337-3DOI Listing

Bioconversion of 5-Hydroxymethylfurfural (HMF) to 2,5-Furandicarboxylic Acid (FDCA) by a Native Obligate Aerobic Bacterium, Acinetobacter calcoaceticus NL14.

Appl Biochem Biotechnol 2020 May 11. Epub 2020 May 11.

Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People's Republic of China.

2,5-Furandicarboxylic acid (FDCA), one of the top biomass-based platform chemical, is highly promising for resins and polymers, and it can be prepared from the bio-oxidation of hydroxymethyl furfural (HMF), which can be obtained mainly from lignocellulosic glucose that has a high production potential from not edible biomass.A native strain, Acinetobacter calcoaceticus NL14, that could convert HMF into FDCA is used for combining degradation and fermentation by consolidated bioprocessing (CBP). In this study, it was observed that the initial HMF concentration and pH neutralizer played important roles in the bioconversion of HMF, 5 g/L of HMF could be converted by 100% within 48 h with 0. Read More

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http://dx.doi.org/10.1007/s12010-020-03325-7DOI Listing

Evaluation of Hydrothermal Pretreatment on Lignocellulose-Based Waste Furniture Boards for Enzymatic Hydrolysis.

Appl Biochem Biotechnol 2020 May 11. Epub 2020 May 11.

Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.

Three typical waste furniture boards, including fiberboard, chipboard, and blockboard, were pretreated with conventional hydrothermal method. The responses of chemical composition, physicochemical morphology, and performances of enzymatic hydrolysis were evaluated. Results indicated the almost complete hemicellulose removal at higher pretreatment temperatures, the enhanced crystallinity index, and disordered morphology of the pretreated substrates indicated that the hydrothermal pretreatment deconstructed these boards well. Read More

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http://dx.doi.org/10.1007/s12010-020-03315-9DOI Listing

In Vitro N-Terminal Acetylation of Bacterially Expressed Parvalbumins by N-Terminal Acetyltransferases from Escherichia coli.

Appl Biochem Biotechnol 2020 May 11. Epub 2020 May 11.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia.

Most eukaryotic proteins are N-terminally acetylated (Nt-acetylated) by specific N-terminal acetyltransferases (NATs). Although this co-/post-translational protein modification may affect different aspects of protein functioning, it is typically neglected in studies of bacterially expressed eukaryotic proteins, lacking this modification. To overcome this limitation of bacterial expression, we have probed the efficiency of recombinant Escherichia coli NATs (RimI, RimJ, and RimL) with regard to in vitro Nt-acetylation of several parvalbumins (PAs) expressed in E. Read More

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http://dx.doi.org/10.1007/s12010-020-03324-8DOI Listing
May 2020
1.735 Impact Factor

Enzymatic Synthesis of β-Sitosterol Laurate by Candida rugosa Lipase AY30 in the Water/AOT/Isooctane Reverse Micelle.

Appl Biochem Biotechnol 2020 May 9. Epub 2020 May 9.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China.

Phytosterols are regarded as compounds able to reduce total and low-density lipoprotein cholesterol in the blood, and their esterified derivatives could help to improve the effectiveness of this function. In the present study, the water/sodium 1,4-bis-2-ethylhexylsulfosuccinate (AOT)/isooctane reverse micelle (RM) system was set up as a reaction medium for Candida rugosa lipase AY30 (CRL AY30) to synthesize β-sitosterol laurate (β-SLE). The product was identified by TLC, FT-IR, and HPLC-APCI-QqQ-MS/MS and quantified by HPLC. Read More

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http://dx.doi.org/10.1007/s12010-020-03302-0DOI Listing

Expression of Highly Active Bacterial Phospholipase A in Yeast Using Intein-Mediated Delayed Protein Autoactivation.

Appl Biochem Biotechnol 2020 May 9. Epub 2020 May 9.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center "Kurchatov Institute", 1st Dorozhniy proezd, 1, Moscow, Russia, 117545.

Phospholipase A (PLA) has found extensive use in industry. However, recombinant PLA production in different expression systems is a difficult task because of its toxicity to cell membranes. We report here the development of an effective method for production of highly active PLA from Streptomyces violaceoruber strain A-2688 in the yeast Saccharomyces cerevisiae. Read More

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http://dx.doi.org/10.1007/s12010-020-03333-7DOI Listing

Microalgae Cultivation Using Screened Liquid Dairy Manure Applying Different Folds of Dilution: Nutrient Reduction Analysis with Emphasis on Phosphorus Removal.

Appl Biochem Biotechnol 2020 May 8. Epub 2020 May 8.

Department of Biological Engineering, University of Idaho, 875 Perimeter Drive, Moscow, ID, 83844, USA.

A number of dairies in southern Idaho employed stationary inclined screens to separate large solid particles out of liquid dairy manure. In this way, the total solid content of the liquid dairy manure can drop about 20%. Solids in dairy wastewater cause high turbidities, which could block the incident light, a key factor in the microalgae cultivation process using wastewaters as culture media. Read More

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http://dx.doi.org/10.1007/s12010-020-03316-8DOI Listing

Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1.

Appl Biochem Biotechnol 2020 May 7. Epub 2020 May 7.

Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.

To valorize starchy waste from rice noodle factory, bioconversion of gelatinized starchy waste (GSW) to value-added product as L(+)-lactic acid, the monomer for polylactate synthesis, was investigated using amylolytic lactic acid bacterium, Enterococcus faecium K-1. Screening for appropriate nitrogen source to replace expensive organic nitrogen sources revealed that corn steep liquor (CSL) was the most suitable regarding high efficacy for L(+)-LA achievement and low-cost property. The successful applying statistic experimental design, Plackett-Burman design incorporated with central composite design (CCD), predicted the maximum L(+)-LA of 93. Read More

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http://dx.doi.org/10.1007/s12010-020-03314-wDOI Listing
May 2020
1.735 Impact Factor

Stabilization of Glycosylated β-Glucosidase by Intramolecular Crosslinking Between Oxidized Glycosidic Chains and Lysine Residues.

Appl Biochem Biotechnol 2020 May 7. Epub 2020 May 7.

Departamento de Biotecnologia y Microbiologia de los Alimentos, Instituto de Investigación en Ciências de los Alimentos, C/Nicolás Cabrera 9, Campus UAM Cantoblanco, Madrid, Spain.

Many industrial enzymes can be highly glycosylated, including the β-glucosidase enzymes. Although glycosylation plays an important role in many biological processes, such chains can cause problems in the multipoint immobilization techniques of the enzymes, since the glycosylated chains can cover the reactive groups of the protein (e.g. Read More

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http://dx.doi.org/10.1007/s12010-020-03321-xDOI Listing

Micro-oxygen Process Improved Synthesis of PHAs with Undomesticated Excess Sludge.

Appl Biochem Biotechnol 2020 May 7. Epub 2020 May 7.

Department of Municipal Engineering, Guangzhou University, Guangzhou, China.

Polyhydroxyalkanoates (PHAs) are a potential substitute for traditional plastics. Synthesis of PHAs using excess sludge without additional domestication as a mixed microbial culture can reduce production costs. PHAs were synthesized using excess sludge (R1) from a continuous flow system performing simultaneous nitrification/denitrification and phosphorus removal. Read More

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http://dx.doi.org/10.1007/s12010-020-03322-wDOI Listing

N-Terminal Fused Signal Peptide Prompted Extracellular Production of a Bacillus-Derived Alkaline and Thermo Stable Xylanase in E. coli Through Cell Autolysis.

Appl Biochem Biotechnol 2020 May 7. Epub 2020 May 7.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Engineering Research Center for Bio-enzyme Catalysis, College of Life Sciences, Hubei University, NO. 368 Youyi Road, Wuchang District, Wuhan, 430062, Hubei, China.

Xylanases are extensively used as industrial enzymes for its ability of hydrolyzing xylan to oligosaccharides. Here, XynHB, a thermo and alkaline stable xylanase derived from Bacillus pumilus HBP8, was extracellularly produced in E. coli cells through N-terminal-fused signal peptides. Read More

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http://dx.doi.org/10.1007/s12010-020-03323-9DOI Listing

Automated Pressurized Liquid Extraction of Microbial Lipids from Oleaginous Yeasts.

Appl Biochem Biotechnol 2020 May 6. Epub 2020 May 6.

Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.

The lipids produced by oleaginous microbes are considered sustainable resources for biofuels. To facilitate controlled lipid production and lipid analysis, more efficient lipid extraction methods are required. This study describes the automated pressurized liquid extraction (APLE) method for lipid extraction from dried cells of the oleaginous yeast species Rhodosporidium toruloides and Cryptococcus curvatus. Read More

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http://dx.doi.org/10.1007/s12010-020-03331-9DOI Listing
May 2020
1.735 Impact Factor

Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases.

Appl Biochem Biotechnol 2020 May 6. Epub 2020 May 6.

Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Av. PH Rolfs, s/n, Viçosa, MG, 36570-900, Brazil.

This work intended to prospect new phytase-producing organisms. In silico genomic analyses allowed the selection of twelve potential phytase-producing fungi. Based on gene sequence, it was possible to identify four well-defined groups of phytate-degrading enzymes: esterase-like, β-propeller phytases (βPP), phosphoglycerate mutase-like, and phytases of the histidine acid phosphatases (HAP) family. Read More

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http://dx.doi.org/10.1007/s12010-020-03309-7DOI Listing

A Novel Cellobiohydrolase I (CBHI) from Penicillium digitatum: Production, Purification, and Characterization.

Appl Biochem Biotechnol 2020 May 6. Epub 2020 May 6.

Department of Biochemistry, State University of Maringá, Av. Colombo, 5790, Maringá, PR, 87020-900, Brazil.

A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1. Read More

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http://dx.doi.org/10.1007/s12010-020-03307-9DOI Listing

Stronger Stimulation of Waste Activated Sludge Anaerobic Fermentation by a Particular Amount of Micron-Sized Zero Valent Iron.

Appl Biochem Biotechnol 2020 May 6. Epub 2020 May 6.

College of Architecture and Environment, Sichuan University, Chengdu, 610065, China.

An emerging contaminant, micron-sized zero valent iron (mZVI) has been reported to be accumulated in waste activated sludge (WAS). In the present study, the potential effects of mZVI on WAS anaerobic fermentation performance, as well as the shift of the microbial community composition and function, were assessed. Results from batch experiments indicated that a particular concentration of mZVI (1. Read More

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http://dx.doi.org/10.1007/s12010-020-03339-1DOI Listing

Pretreatment and Detoxification of Acid-Treated Wood Hydrolysates for Pyruvate Production by an Engineered Consortium of Escherichia coli.

Appl Biochem Biotechnol 2020 May 5. Epub 2020 May 5.

School of Chemical, Material and Biomedical Engineering, University of Georgia, Athens, GA, 30602, USA.

The biorefinery concept makes use of renewable lignocellulosic biomass to produce commodities sustainably. A synthetic microbial consortium can enable the simultaneous utilization of sugars such as glucose and xylose to produce biochemicals, where each consortium member converts one sugar into the target product. In this study, woody biomass was used to generate glucose and xylose after pretreatment with 20% (w/v) sulfuric acid and 60-min reaction time. Read More

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http://dx.doi.org/10.1007/s12010-020-03320-yDOI Listing

In Silico and In Vitro Analyses of Glucosamine and Indole Acetaldehyde Inhibit Pathogenic Regulator Gene phcA of Ralstonia solanacearum, a Causative Agent of Bacterial Wilt of Tomato.

Appl Biochem Biotechnol 2020 May 4. Epub 2020 May 4.

C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus Bardoli, Surat, Gujarat, 394350, India.

In this study, medicinal plant (Solanum surattense)-associated bacteria were isolated and their extracellular secondary metabolites were extracted. Dual-plate application of crude secondary metabolites proved that SSL2I and SSL5 had a good inhibitory activity against Ralstonia solanacearum. These biocontrol bacteria were identified as Bacillus subtilis and Bacillus velezensis by 16S rRNA gene sequencing analysis. Read More

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http://dx.doi.org/10.1007/s12010-020-03328-4DOI Listing

Screening High CO-Tolerant Oleaginous Microalgae from Genera Desmodesmus and Scenedesmus.

Appl Biochem Biotechnol 2020 May 1. Epub 2020 May 1.

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Microalgae from genus Scenedesmus sensu lato (including Desmodesmus and Scenedesmus) were reported to be particularly suitable candidates for CO biomitigation. In this study, 16 strains from Scenedesmus sensu lato were obtained from different climate zones of China and their phylogenetic positions were determined. Seven strains out of the 16 showed high CO tolerance and grew much faster under 20% CO than air condition. Read More

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http://dx.doi.org/10.1007/s12010-020-03319-5DOI Listing

Biodegradation of Reactive Orange 16 Dye in Microbial Fuel Cell: An Innovative Way to Minimize Waste Along with Electricity Production.

Appl Biochem Biotechnol 2020 Apr 27. Epub 2020 Apr 27.

Department of Chemical Engineering & Technology, Centre of Advanced Study, Indian Institute of Technology, BHU, Varanasi, 221005, India.

Microbial fuel cell (MFC) is an attractive green technology which harnesses the power of microorganism for the production of electricity along with bioremediation of waste. However, the bioremediation of the high concentration of dye wastewater in MFC remains unclear. In present study, double-chambered MFC inoculated with mixed bacterial consortium was used for bioremediation of reactive orange 16 (RO-16) dyes at a very high and variable concentration range of 100 to 1000 ppm. Read More

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http://dx.doi.org/10.1007/s12010-020-03306-wDOI Listing
April 2020
1.735 Impact Factor

Enhanced Bioethanol Fermentation by Sonication Using Three Yeasts Species and Kariba Weed (Salvinia molesta) as Biomass Collected from Lake Victoria, Uganda.

Appl Biochem Biotechnol 2020 Apr 27. Epub 2020 Apr 27.

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea.

Kariba weed (Salvinia molesta) was used as biomass feedstock for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate were produced using thermal acid hydrolysis, sonication, and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of 12% (w/v) Kariba weed slurry were evaluated as 200 mM HNO at 121 °C for 60 min yielding 10. Read More

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http://dx.doi.org/10.1007/s12010-020-03305-xDOI Listing

Surfactant as an Additive for the Recovery of Potent Antioxidants from Garcinia mangostana Pericarps Using a Polymer/Salt Aqueous Biphasic System.

Appl Biochem Biotechnol 2020 Apr 25. Epub 2020 Apr 25.

Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan, 320, Taiwan.

Garcinia mangostana pericarp is a good source of natural antioxidants with numerous functional properties. The conventional approaches for the recovery of antioxidants from Garcinia mangostana pericarp require long processing time and high temperature, which may cause degradation or loss of bioactivity of antioxidants, and often result in low recovery efficiency. In this study, the extraction of antioxidants from Garcinia mangostana pericarp was investigated using a polyethylene glycol (PEG)/citrate aqueous biphasic system (ABS) with the addition of surfactants. Read More

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http://dx.doi.org/10.1007/s12010-020-03284-zDOI Listing

Metal-Organic Frameworks Conjugated Lipase with Enhanced Bio-catalytic Activity and Stability.

Appl Biochem Biotechnol 2020 Apr 22. Epub 2020 Apr 22.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.

Covalent immobilization of lipase onto a solid carrier is an effective way to enhance stability. Immobilization inhibits the activity of lipase due to decreased flexibility of enzyme structure via the covalent bond. In this study, monomer of the metal-organic frameworks (MOFs) material ZIF-8 (2-methyl imidazole-4-carboxylic acid) was innovatively used as a chemical modifier of Candida nrugosa lipase (CRL). Read More

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http://dx.doi.org/10.1007/s12010-020-03268-zDOI Listing
April 2020
1.735 Impact Factor

Synthesizing Chiral Drug Intermediates by Biocatalysis.

Appl Biochem Biotechnol 2020 Apr 22. Epub 2020 Apr 22.

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

The chiral feature is a critical factor for the efficacy and safety of many therapeutic agents. At present, about 57% of marketed drugs are chiral drugs and about 99% of purified natural products are chiral compounds. There has been a tremendous potential of functional microorganisms and biocatalysts derived from them for the bioconversion of synthetic chemicals into drugs with high enantio-, chemo-, and regio-selectivities. Read More

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http://dx.doi.org/10.1007/s12010-020-03272-3DOI Listing

Delignification of Pinecone and Extraction of Formic Acid in the Hydrolysate Produced by Alkaline Fractionation.

Appl Biochem Biotechnol 2020 Apr 8. Epub 2020 Apr 8.

Department of Chemical Engineering and Interagency Convergence Energy on New Biomass Industry, Hankyong National University, 327, Jungang-ro, Anseong-si, Gyeonggi-do, 17579, South Korea.

The objectives of our research are to investigate the concept of delignification from pinecone through alkaline fractionation and then extraction of formic acid from the hydrolysate through esterification using ethanol. The pinecone is considered a promising material because of its relatively higher lignin content (35.80%) than other lignocellulosic biomass. Read More

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http://dx.doi.org/10.1007/s12010-020-03311-zDOI Listing

Isolation and Characterization of a Novel myovirus Infecting Shigella dysenteriae from the Aeration Tank Water.

Appl Biochem Biotechnol 2020 Apr 8. Epub 2020 Apr 8.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.

The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. Read More

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http://dx.doi.org/10.1007/s12010-020-03310-0DOI Listing

Comprehensive Optimization of Culture Conditions for Production of Biomass-Hydrolyzing Enzymes of Trichoderma SG2 in Submerged and Solid-State Fermentation.

Appl Biochem Biotechnol 2020 Apr 4. Epub 2020 Apr 4.

Bioprocessing and Biofuel Research Lab, Department of Biology and Environmental Science, Auburn University at Montgomery, Montgomery, AL, USA.

Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for bioconversion of lignocellulose biomass. Overall, a mixture of paper powder and switchgrass was most suited for production of BHE in submerged fermentation (SmF). Read More

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http://dx.doi.org/10.1007/s12010-020-03258-1DOI Listing

Development of a Reversible Indicator Displacement Assay Based on the 1-(2-Pyridylazo)-2-naphthol for Colorimetric Determination of Cysteine in Biological Samples and Its Application to Constructing the Paper Test Strips and a Molecular-Scale Set/Reset Memorized Device.

Appl Biochem Biotechnol 2020 Apr 2. Epub 2020 Apr 2.

Department of Chemistry, Payame Noor University, Tehran, 19395-4697, Islamic Republic of Iran.

A new colorimetric chemosensor for naked-eye detection and determination of cysteine (Cys) based on indicator displacement assay (IDA) was designed using 1-(2-pyridylazo)-2-naphthol (PAN). The indicator exchange occurred between PAN and Cys by the addition of Cys to the Cu(PAN) complex, which is accomplished by an immediate visible color change from magenta to yellow, in the solution phase and paper-based test strips. The proposed method exhibits 0. Read More

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http://dx.doi.org/10.1007/s12010-019-03165-0DOI Listing

Purification and Biochemical Characterization of a Tyrosine Phenol-lyase from Morganella morganii.

Appl Biochem Biotechnol 2020 Mar 31. Epub 2020 Mar 31.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Tyrosine phenol-lyase (TPL) is a valuable and cost-effective biocatalyst for the biosynthesis of L-tyrosine and its derivatives, which are valuable intermediates in the pharmaceutical industry. A TPL from Morganella morganii (Mm-TPL) was overexpressed in Escherichia coli and characterized. Mm-TPL was determined as a homotetramer with molecular weight of 52 kDa per subunit. Read More

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http://dx.doi.org/10.1007/s12010-020-03301-1DOI Listing

Lipid Induction in Scenedesmus abundans GH-D11 by Reusing the Volatile Fatty Acids in the Effluent of Dark Anaerobic Fermentation of Biohydrogen.

Appl Biochem Biotechnol 2020 Mar 28. Epub 2020 Mar 28.

Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan.

This study aims to investigate the efficacy of lipid induction in Scenedesmus abundans by adding the effluent from dark fermentation of biohydrogen production. Four sets of experiments were conducted: control (sufficient nitrogen), nitrogen depletion, low concentration (0.3×) effluent addition, and high concentration (0. Read More

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http://dx.doi.org/10.1007/s12010-020-03294-xDOI Listing

Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.

Appl Biochem Biotechnol 2020 Mar 27. Epub 2020 Mar 27.

College of Life Science and Technology, Guangxi University, Nanning, 530004, China.

This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold. Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase. All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type. Read More

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http://dx.doi.org/10.1007/s12010-020-03308-8DOI Listing

Urinary Metabolomics and Biochemical Analysis of Antihyperglycemic Effect of Ficus deltoidea Jack Varieties in Streptozotocin-Nicotinamide-Induced Diabetic Rats.

Appl Biochem Biotechnol 2020 Mar 25. Epub 2020 Mar 25.

Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, 42300, Bandar Puncak Alam, Selangor, Malaysia.

Patients are turning into herbs for the management of diabetes, which cause increasing in the demand of plant-based alternative medicines. Ficus deltoidea or locally known as "Mas Cotek" in Malaysia is a famous herbal plant. However, many varieties of F. Read More

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http://dx.doi.org/10.1007/s12010-020-03304-yDOI Listing

Characterization of Denitrifying Community for Application in Reducing Nitrogen: a Comparison of nirK and nirS Gene Diversity and Abundance.

Appl Biochem Biotechnol 2020 Mar 25. Epub 2020 Mar 25.

College of Resources and Environment, Southwest University, No. 2, Tiansheng Street, Beibei, Chongqing, 400716, People's Republic of China.

Studies have shown that the addition of biochar to agricultural soils has the potential to mitigate climate change by decreasing nitrous oxide (NO) emissions resulting from denitrification. Rice paddy field soils have been known to have strong denitrifying activity, but the response of microbes to biochar for weakening denitrification in rice paddy field soils is not well known. In this work, compared with the chemical fertilizer alone, the chemical fertilizer + 20 t hm biochar fertilizer slightly decreased denitrifying the nitrite reductase activity (S-NiR) and NO emission without statistic difference, whereas the chemical fertilizer + 40 t hm biochar significantly boosted them. Read More

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http://dx.doi.org/10.1007/s12010-020-03250-9DOI Listing

Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method.

Appl Biochem Biotechnol 2020 Mar 25. Epub 2020 Mar 25.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and safe 96-well microplate assay method with sodium nitrite (NaNO) as chromogenic reagent was proposed and optimized. The optimum assay conditions were as follows: the detection wavelength was 420 nm, the concentration of NaNO solution was 6. Read More

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http://dx.doi.org/10.1007/s12010-020-03303-zDOI Listing

Tyrosinase Inhibition by 4-Substituted Benzaldehydes with Electron-Withdrawing Groups.

Appl Biochem Biotechnol 2020 Mar 24. Epub 2020 Mar 24.

Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720-3112, USA.

The oxidation of 4-t-butylcatechol catalyzed by mushroom tyrosinase was inhibited by 4-bromobenzaldehyde, 4-chlorobenzaldehyde, 4-fluorobenzaldehyde, 4-cyanobenzaldehyde, and 4-nitrobenzaldehyde with 50% inhibitory concentrations of 114 μM, 175 μM, 387 μM, 822 μM, and 1846 μM, respectively. The inhibition kinetics were analyzed by Dixon plots, which indicated that a series of 4-hallogenated benzaldehydes acted as partial noncompetitive inhibitors in the same manner as benzaldehyde. Although β values were decreased with an increase of the tyrosinase inhibitory activity, full inhibition could not be observed. Read More

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http://dx.doi.org/10.1007/s12010-020-03295-wDOI Listing

Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.

Appl Biochem Biotechnol 2020 Mar 23. Epub 2020 Mar 23.

Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea.

We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars. Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp. KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes. Read More

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http://dx.doi.org/10.1007/s12010-020-03297-8DOI Listing

Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation.

Appl Biochem Biotechnol 2020 Mar 23. Epub 2020 Mar 23.

Department of Food Science & Technology, College of Bioresource Science, Nihon University, Fujisawa, Kanagawa, 252-8510, Japan.

Liquid marble (LM), a non-stick drop coated with micro- or nano-scale particles, has great potential in a wide range of applications. LMs have an advantageous feature in which gas or vapor can freely transport through their particle shell; therefore, it makes them an ideal candidate to be utilized as microbioreactor containing aerobic microorganisms. In this study, safer and more biocompatible LMs were successfully prepared using a food-grade calcium stearate microparticle as a stabilizer. Read More

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http://dx.doi.org/10.1007/s12010-020-03299-6DOI Listing

Polyvinyl Alcohol/Hydroxyethylcellulose Containing Ethosomes as a Scaffold for Transdermal Drug Delivery Applications.

Appl Biochem Biotechnol 2020 Mar 21. Epub 2020 Mar 21.

Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.

This study aims to develop scaffold for transdermal drug delivery method (TDDM) using electrospinning technique from polyvinyl alcohol (PVA) and hydroxyethylcellulose (HEC). The fluorescein isothiocyanate (FITC) loaded on ethosomes (FITC@Eth) was used as a drug model. The prepared PVA/HEC/FITC@Eth scaffold was characterized via scanning electron microscope (SEM) that show morphology change by adding FITC@Eth. Read More

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http://dx.doi.org/10.1007/s12010-020-03282-1DOI Listing

Refolding with Simultaneous Purification of Recombinant Serratia marcescens Lipase by One-Step Ultrasonication Process.

Appl Biochem Biotechnol 2020 Mar 21. Epub 2020 Mar 21.

Research and Development Center, Shanghai Research Institute of Chemical Industry Co., Ltd., 345 East Yunling Road, Shanghai, 200062, China.

A new lipase from Serratia marcescens SRICI-01 (Trx-SmL) was successfully overexpressed in Escherichia coli with thioredoxin (Trx) fusion tag. Intriguingly, the concentration of potassium phosphate buffer (KPB) showed significant impact on the aggregation state of Trx-SmL during ultrasonic disruption. The proportion of inclusion bodies increased dramatically with the increase of KPB concentration from almost completely soluble in 10 mM KPB to insoluble in 200 mM KPB. Read More

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http://dx.doi.org/10.1007/s12010-019-03172-1DOI Listing