32,665 results match your criteria Applied and Environmental Microbiology[Journal]


Establishment limitation constrains the abundance of lactic acid bacteria in the Napa cabbage phyllosphere.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Tufts University, Department of Biology, Medford, Massachusetts, USA

Patterns of phyllosphere diversity have become increasingly clear with high-throughput sequencing surveys, but the processes that control phyllosphere diversity are still emerging. Through a combination of lab and field experiments using Napa cabbage and lactic acid bacteria (LAB), we examined how dispersal and establishment processes shape the ecological distributions of phyllosphere bacteria. We first determined the abundance and diversity of LAB on Napa cabbage grown at three sites using both culture-based approaches and 16S rRNA gene amplicon sequencing. Read More

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http://dx.doi.org/10.1128/AEM.00269-19DOI Listing

Exploring bacterial and fungal endophytic microbiomes of .

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Torun, Poland

We examined a non-mycorrhizal halophyte associated with specific and unique endophytic bacteria and fungi. Microbial community structure was analyzed at two sites differing in salinization history (anthropogenic and naturally saline site), in contrasting seasons (spring and fall) and in two plant organs (shoots and roots) 16S rRNA and ITS amplicon sequencing. We observed distinct communities at the two sites, and in shoots and roots, while the season was of no importance. Read More

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http://dx.doi.org/10.1128/AEM.00305-19DOI Listing
April 2019
1 Read

Congo Red fluorescence for rapid characterization of synthetic curli systems.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States

Curli are amyloid proteins, assembled into extracellular polymeric fibers by bacteria during biofilm formation. The beta-sheet rich protein CsgA, the primary structural component of the fibers, is secreted through dedicated machinery, and self-assembles into cell-anchored fibers many times longer than the cell. Here, we have developed an fluorescence assay for curli production that exploits the fluorescent properties of Congo Red dye when bound to amyloid, allowing for rapid and robust curli quantification. Read More

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http://dx.doi.org/10.1128/AEM.00434-19DOI Listing

Microbial network and soil properties are changed in bacterial wilt susceptible soil.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

College of Life Science and Technology, Huazhong Agricultural University, China

Bacterial wilt disease is a devastating disease of crops, which leads to huge economic loss worldwide. It is hypothesized bacterial wilt occurrence might possibly relate to changes in soil chemical properties and microbial interactions. In this study, we compared the soil chemical properties and microbial network structure of a healthy soil (HS) and a bacterial wilt susceptible soil (BWS). Read More

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http://dx.doi.org/10.1128/AEM.00162-19DOI Listing
April 2019
1 Read

Honeybee-specific lactic acid bacterial supplements have no effect on American foulbrood infected honeybee colonies.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, 750 07 Sweden.

, causative agent of American Foulbrood (AFB), is the primary bacterial pathogen affecting honeybees and beekeeping. The main methods for controlling AFB are incineration of diseased colonies or prophylactic antibiotic treatment (e.g. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00606-19
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http://dx.doi.org/10.1128/AEM.00606-19DOI Listing
April 2019
1 Read

The plant host induces antibiotic production to select the most beneficial colonizers.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Molecular Genetics, Weizmann Institute of Science

Microbial ecosystems tightly associated with a eukaryotic host are wide-spread in nature. The genetic and metabolic networks of the eukaryotic hosts and the associated microbes have co-evolved to form a symbiotic relationship. Both the Gram-positive and the Gram-negative can form biofilms on plant roots, and thus can serve as a model system for the study of interspecies interactions in a host-associated ecosystem. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00512-19
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http://dx.doi.org/10.1128/AEM.00512-19DOI Listing
April 2019
1 Read

Mode of action of GH30-7 reducing-end xylose-releasing exo-xylanase A (Xyn30A) from the filamentous fungus .

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Bioconversion Group, Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Hiroshima, Japan.

In this study, we characterized the mode of action of reducing-end xylose-releasing exo-xylanase (Rex), which belongs to the glycoside hydrolase family (GH) 30-7. GH30-7 Rex, isolated from the cellulolytic fungus (Xyn30A), exists as a dimer. The purified Xyn30A released xylose from the linear xylooligosaccharides (XOSs) 3 to 6 xylose units in length with similar kinetic constants. Read More

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http://dx.doi.org/10.1128/AEM.00552-19DOI Listing

MnoSR is a bona fide two-component system involved in methylotrophic metabolism in .

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India

() and several other mycobacteria are able to utilize methanol as the sole source of carbon and energy. We recently showed that N,N-dimethyl-p-nitrosoaniline (NDMA)-dependent methanol dehydrogenase (Mno) is essential for the growth of on methanol. Although Mno from this bacterium shares high homology with other known methanol dehydrogenases, methanol metabolism in differs significantly from other described methylotrophs. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00535-19
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http://dx.doi.org/10.1128/AEM.00535-19DOI Listing
April 2019
1 Read

Synthesis of d-amino acid-containing dipeptides using the adenylation domains of nonribosomal peptide synthetase.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan

Recent papers have reported dipeptides containing d-amino acid to have novel effects, which cannot be observed with ll-dipeptides and are expected to be novel functional compounds for pharmaceuticals and food additives. Although the function of d-amino acid-containing dipeptides is gaining more attention, there are few reports on the synthetic enzymes that can accept d-amino acid as a substrate, and synthetic methods for d-amino acid-containing dipeptides are still not constructed. Previously, we developed a chemoenzymatic system for amide synthesis, which comprised enzymatic activation and a subsequent nucleophilic substitution reaction. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00120-19
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http://dx.doi.org/10.1128/AEM.00120-19DOI Listing
April 2019
1 Read

Post-translational targeting of a recombinant protein promotes its efficient secretion into the periplasm.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden

Many recombinant proteins that are produced in have to be targeted to the periplasm to be functional. N-terminal signal peptides can be used to direct recombinant proteins to the membrane embedded Sec-translocon, a multiprotein complex that translocates proteins across the membrane into the periplasm. We have recently shown that the co-translational targeting of the single-chain variable antibody fragment BL1 saturates the capacity of the Sec-translocon leading to impaired translocation of secretory proteins and protein misfolding/aggregation in the cytoplasm. Read More

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http://dx.doi.org/10.1128/AEM.00671-19DOI Listing

Erratum for Wegner et al., "Biogeochemical Regimes in Shallow Aquifers Reflect the Metabolic Coupling of the Elements Nitrogen, Sulfur, and Carbon".

Appl Environ Microbiol 2019 May 18;85(9). Epub 2019 Apr 18.

Chair of Aquatic Geomicrobiology, Institute of Biodiversity, Friedrich Schiller University, Jena, Germany.

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http://dx.doi.org/10.1128/AEM.00502-19DOI Listing

Characterization of the bacteriophage φEf11 ORF 28 endolysin: A multi-functional lytic enzyme with properties distinct from all other identified phage endolysins.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Endodontology, Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA

φEf11 is a temperate, bacteriophage that infects strains of The φEf11 genome, encompassing 65 open reading frames (ORFs), is contained within 42,822 bp of DNA. Within this genome, a module of six lysis-related genes was identified. Based upon sequence homology, one of these six genes, ORF 28, was predicted to code for an N- acetylmuramoyl-L-alanine amidase endolysin of 46. Read More

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http://dx.doi.org/10.1128/AEM.00555-19DOI Listing

Wetland sediments host diverse microbial taxa capable of cycling alcohols.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Soil and Crop Sciences, Colorado State University, CO, USA

Alcohols are commonly derived from the degradation of organic matter, and yet are rarely measured in environmental samples. Wetlands in the Prairie Pothole Region (PPR) support extremely high methane emissions and the highest sulfate reduction rates reported to date, likely contributing to a significant proportion of organic matter mineralization in this system. While ethanol and isopropanol concentrations up to 4-5 mM in PPR wetland pore fluids have been implicated in sustaining these high rates of microbial activity, the mechanisms that support alcohol cycling in this ecosystem are poorly understood. Read More

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http://dx.doi.org/10.1128/AEM.00189-19DOI Listing
April 2019
1 Read

Deep Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing .

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, 80521

Bacterial strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolerate large changes in pressure, temperature, and salinity. Here, we used a strain isolated from a natural gas well in the Utica Point Pleasant formation to investigate metabolic and physiological responses to growth under high-pressure subsurface conditions. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00018-19
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http://dx.doi.org/10.1128/AEM.00018-19DOI Listing
April 2019
2 Reads

Biofilm formation and synthesis of antimicrobial compounds by the biocontrol agent QST713 in compost micromodel.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.

QST713 is widely used as a biological control agent for crop protection and disease suppression. This strain is used industrially in France for the protection of against f. , the green mould disease. Read More

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http://dx.doi.org/10.1128/AEM.00327-19DOI Listing

Commensal aggravates acute necrotizing pancreatitis through targeting intestinal epithelial cells.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Gastroenterology, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.

An increase of was previously reported in acute necrotizing pancreatitis (ANP). Here we aimed to investigate whether () MG1655, a commensal belonging to , increased intestinal injury and aggravated ANP in rats. ANP was induced by retrograde injection of 3. Read More

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http://dx.doi.org/10.1128/AEM.00059-19DOI Listing

Blue light photoreceptor gene regulates phototropic response and fruiting-body development in the homothallic ascomycete .

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Plant Pathology and Microbiology, National Taiwan University, No. 1 Roosevelt Road, Sec. 4, Taipei 10617, Taiwan.

, a coprophilous ascomycete, is a homothallic fungus that can undergo sexual differentiation with cellular and morphological changes followed by multicellular tissue development to complete its sexual cycle. In this study, we identified and characterized the blue light photoreceptor gene in white collar-1 photoreceptor (SfWC-1) contains light-oxygen-voltage-sensing (LOV), Per-Arnt-Sim (PAS) and other conserved domains and is homologous to the WC-1 blue-light photoreceptor of The LOV domain of was deleted by homologous recombination using mediated protoplast transformation. The mutant showed normal vegetative growth but produced less carotenoid pigment under illumination. Read More

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http://dx.doi.org/10.1128/AEM.02206-18DOI Listing

Level of spiked virus necessary to correctly assess enteric virus recovery in water matrices.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

School of Public Health, University of Alberta, Edmonton, AB T6G 2G7, Canada

Quantitative microbial risk assessment (QMRA) identifies human enteric viruses in municipal wastewaters as the pathogen group requiring highest log-reductions for various reuse applications. When estimating virus concentration, however, method performance is not well understood, which without inclusion actual risks are likely severely underestimated. To evaluate recovery efficiency for viruses in water, 'known' amounts of virus are often spiked into a water sample and then recovered after a series of analytical procedures. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00111-19
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http://dx.doi.org/10.1128/AEM.00111-19DOI Listing
April 2019
2 Reads

Following the Evolutionary Track of a Highly Specific L-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.

Following the evolutionary track of enzymes can help elucidate how enzymes attain their characteristic functions, such as thermal adaptation and substrate selectivity, during the evolutionary process. Ancestral sequence reconstruction (ASR) is effective for following evolutionary processes if sufficient sequence data are available. Selecting sequences from the data to generate a curated sequence library are necessary for the successful design of artificial proteins by ASR. Read More

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http://dx.doi.org/10.1128/AEM.00459-19DOI Listing

Reversible Gene Expression Control in Using an Optimized CRISPRi System.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China

Many genes in the bacterial pathogen , the causative agent of three plague pandemics, remain uncharacterized, greatly hampering the development of measures for plague prevention and control. Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has been shown to be an effective tool for gene knockdown in model bacteria. In this system, a catalytically-dead Cas9 (dCas9) and a small guide RNA (sgRNA) form a complex, binding to the specific DNA target through base pairing, thereby impeding RNA polymerase binding and causing target gene repression. Read More

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http://dx.doi.org/10.1128/AEM.00097-19DOI Listing
April 2019
1 Read

Changes in fungal communities across a forest disturbance gradient.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

World Agroforestry Centre, East & Southern Africa Region, 13 Elm Road, Woodlands, Lusaka, Zambia

Deforestation has a substantial impact on above ground biodiversity, but the response of below ground soil fungi remains poorly understood. In a tropical montane rainforest in southwestern China, plots were established along a forest degradation gradient ranging from mature and regenerated forests to open land to examine the impacts of forest degradation and deforestation on ecosystem diversity and function. Here, we evaluate the changes in below ground fungal diversity and community composition using a metabarcoding approach. Read More

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http://dx.doi.org/10.1128/AEM.00080-19DOI Listing

Molecular Mechanism of ,-Dimethylformamide Degradation in a sp. Strain DM1.

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China

,-Dimethylformamide (DMF) is one of the most common xenobiotic chemicals and can be easily emitted into the environment, where it causes harm to human beings. Herein, an efficient DMF-degrading strain DM1 was isolated and identified as sp. This strain can use DMF as the sole source of carbon and nitrogen. Read More

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http://dx.doi.org/10.1128/AEM.00275-19DOI Listing
April 2019
1 Read

Decontamination effect of the Spindle and 222-nm krypton-chlorine excilamp combination against pathogens on apples ( Borkh.) and bell peppers ( L.).

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

In this study, we developed a washing system capable of decontaminating fresh produce by combining the Spindle, which detaches microorganisms on sample surfaces, and a 222-nm krypton-chlorine (KrCl) excilamp (Sp-Ex), and investigated their decontamination effect against O157:H7, Typhimurium, and on apple ( Borkh.) and bell pepper ( L.) surfaces. Read More

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http://dx.doi.org/10.1128/AEM.00006-19DOI Listing
April 2019
1 Read

Functional Analysis of LanI Mediated Lantibiotic Immunity in .

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Institute for Molecular Biosciences, *Institute of Physical and Theoretical Chemistry, Goethe University, Frankfurt, Germany

Lantibiotics subtilin and nisin are produced by and , respectively. To prevent toxicity of their own lantibiotic both bacteria express specific immunity proteins called SpaI and NisI. In addition, ABC-transporters SpaFEG and NisFEG prevent lantibiotic toxicity by transporting the respective peptides to the extracellular space. Read More

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http://dx.doi.org/10.1128/AEM.00534-19DOI Listing
April 2019
2 Reads

Comparative Metagenomics Reveals Enhanced Nutrient Cycling Potential After Two Years of Biochar Amendment in a Tropical Oxisol.

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Center for Fundamental and Applied Microbiomics, The Biodesign Institute, Arizona State University, Tempe, AZ, 85281

The complex structural and functional responses of agricultural soil microbial communities to the addition of carbonaceous compounds such as biochar remains poorly understood. This severely limits the predictive ability for both the potential enhancement of soil fertility and greenhouse gas mitigation. In this study, we utilized shotgun metagenomics in order to decipher changes in the microbial community in soil microcosms after 14 days of incubation at 23°C, which contained soils from biochar-amended and control plots cultivated with napiergrass. Read More

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http://dx.doi.org/10.1128/AEM.02957-18DOI Listing
April 2019
2 Reads

Rapid and stable microbial community assembly in the headwaters of third-order stream.

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

University of Georgia, Department of Microbiology, Athens, Georgia, USA

Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stability of microbial communities along the length of a first through third order stream, fine-scale temporal and spatial sampling regimes were employed along McNutt Creek in Athens, Georgia, USA. Read More

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http://dx.doi.org/10.1128/AEM.00188-19DOI Listing
April 2019
2 Reads

Development of a sensitive and specific whole-cell biosensor for arsenic detection.

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Department of Chemical Engineering, University of New Hampshire, Durham NH 03824, USA

Whole-cell biosensors (WCBs) have been designed to detect As(III), but most suffer from poor sensitivity and specificity. In this paper, we developed an arsenic WCB with a positive feedback amplifier in DH5α. The output signal from the reporter mCherry was significantly enhanced by the positive feedback amplifier. Read More

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http://dx.doi.org/10.1128/AEM.00694-19DOI Listing
April 2019
1 Read

Insights into the phylogeny, nodule functioning and biogeographic distribution of microsymbionts nodulating the orphan Kersting's groundnut [ (Harms) Marechal & Baudet] in African soils.

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Chemistry Department, Tshwane University of Technology, Private Bag X680, Pretoria, 0001

Kersting's groundnut [ (Harms) Marechal & Baudet] is a neglected indigenous African legume adapted to growth in N-deficient soils due to its ability to fix atmospheric N via symbiosis with rhizobia. Despite its nutritional and medicinal uses, to date, there is little information on the phylogeny and functional traits of its microsymbionts, an aspect much needed for its conservation and improvement. This study explored the morpho-genetic diversity, phylogenetic relationships and N-fixing efficiency of Kersting's groundnut rhizobial isolates from contrasting environments in Ghana, South Africa and Mozambique. Read More

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http://dx.doi.org/10.1128/AEM.00342-19DOI Listing
April 2019
4 Reads

Specific environmental temperature and relative humidity conditions and grafting affect the persistence and dissemination of in tomato plant tissues.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Department of Plant Pathology, The Ohio State University, OARDC, Wooster, OH.

Little is known about the abiotic factors contributing to the pre-harvest persistence of in tomato tissues. Therefore, we investigated the effects of specific environmental conditions and contamination methods on the persistence and dissemination of subsp. serotype Typhimurium (JSG626) in tomato plants. Read More

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http://dx.doi.org/10.1128/AEM.00403-19DOI Listing
March 2019
2 Reads
3.668 Impact Factor

Microbial community analysis provides insights into the effects of tetrahydrofuran on 1,4-dioxane biodegradation.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

Department of Civil and Environmental Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL 32310, USA

Tetrahydrofuran (THF) is known to induce the biodegradation of dioxane (dioxane), an emerging contaminant, but the mechanisms by which THF affects dioxane biodegradation in microbial communities is not well understood. To fill this knowledge gap, changes in microbial community structure in microcosm experiments with synthetic media and landfill leachate were examined over time using 16S rRNA gene amplicon sequencing and functional gene quantitative polymerase chain reaction assays. The overarching hypothesis being tested was that THF promoted dioxane biodegradation by increasing the abundance of dioxane-degrading bacteria in the consortium. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00244-19
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http://dx.doi.org/10.1128/AEM.00244-19DOI Listing
March 2019
2 Reads

Mapping the neutralizing epitopes of enterotoxigenic (ETEC) K88 (F4) fimbrial adhesin and major subunit FaeG.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA

Enterotoxigenic (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and post-weaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins can be effective against diarrhea in young pigs. However, developing a vaccine to broadly protect against various ETEC virulence determinants has been proven challenging. Read More

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http://dx.doi.org/10.1128/AEM.00329-19DOI Listing
March 2019
1 Read

Development of a CRISPR/Cas9 system for gene editing.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

National Bioenergy Center, National Renewable Energy Laboratory (NREL), Golden, CO 80401 USA

Methanotrophic bacteria play a crucial role in the Earth's biogeochemical cycle and have the potential to be employed in industrial biomanufacturing processes due to their capacity to use natural gas- and biogas-derived methane as a sole carbon and energy source. Advanced gene editing systems have the potential to enable rapid, high-throughput methanotrophic genetics and biocatalyst development. To this end, we employed a series of broad-host range expression plasmids to construct a conjugatable CRISPR/Cas9 gene editing system in (Bath). Read More

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http://dx.doi.org/10.1128/AEM.00340-19DOI Listing
March 2019
2 Reads

6-Hydroxypseudooxynicotine dehydrogenase delivers electrons to electron transfer flavoprotein during nicotine degradation by S33.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao 266237, People's Republic of China

S33 degrades nicotine via a novel hybrid of the pyridine and the pyrrolidine pathways. The hybrid pathway consists of at least six steps involved in oxidoreductive reactions before the -heterocycle can be broken down. Collectively, the six steps allow electron transfer from nicotine and its intermediates to the final acceptor O via the electron transport chain (ETC). Read More

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http://dx.doi.org/10.1128/AEM.00454-19DOI Listing

Efficient synthesis of methyl 3-acetoxypropionate by a newly identified Baeyer-Villiger monooxygenase.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P.R. China

Baeyer-Villiger monooxygenases (BVMOs) are an emerging class of promising biocatalysts for the oxidation of ketones to prepare corresponding esters or lactones. Although many BVMOs have been reported, the development of highly efficient enzymes for use in industrial applications is desirable. In this work, we identified a BVMO from (BVMO) with a high affinity toward aliphatic methyl ketones ( < 3. Read More

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http://dx.doi.org/10.1128/AEM.00239-19DOI Listing
March 2019
1 Read

High prevalence of fluoroquinolone-resistant in sheep and increased counts in the bile and gallbladder of sheep medicated with tetracycline in feed.

Appl Environ Microbiol 2019 Mar 29. Epub 2019 Mar 29.

Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA

is a major foodborne pathogen in humans and a significant cause of abortion in sheep. Although ruminants are increasingly recognized as important reservoirs for , limited information is available about the molecular epidemiology and antimicrobial resistance (AMR) profiles of sheep Here we describe a two-trial study that examined profiles in sheep and determined whether in-feed tetracycline influenced the distribution and AMR profiles of Each trial involved 80 commercial sheep naturally infected with , 40 of which were medicated with tetracycline in feed, while the other 40 received feed without antibiotics. Fecal and bile samples were collected for the isolation of The bacterial isolates were analyzed for antimicrobial susceptibility and genotypes. Read More

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http://dx.doi.org/10.1128/AEM.00008-19DOI Listing
March 2019
2 Reads

Biofilm constructing variants of PsJN outcompete the wild-type form in free-living and static conditions but not .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Unité EA 4707 Résistance Induite et Bioprotection des Plantes (RIBP), SFR Condorcet FR CNRS 3417, Université de Reims Champagne-Ardenne, Reims, France

Members of the genus colonize diverse ecological niches. Among the plant-associated strains, PsJN is an endophyte with a broad host range. In spatially structured environment (unshaken broth cultures), biofilm-constructing specialists of PsJN colonizing the air-liquid interface arose at high frequency. Read More

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http://dx.doi.org/10.1128/AEM.02670-18DOI Listing
March 2019
1 Read

Multilocus sequence analysis, a rapid and accurate tool for taxonomic classification, evolutionary relationship and population biology of the genus .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China.

The genus comprises a group of marine-dwelling species with worldwide distribution. Several species are regarded as causative agents of food spoilage and opportunistic pathogens of human diseases. In this study, a standard multilocus sequence analysis (MLSA) based on six protein-coding genes (, , , , and ) was established as a rapid and accurate identification tool in fifty-nine type strains. Read More

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http://dx.doi.org/10.1128/AEM.03126-18DOI Listing
March 2019
1 Read

Composition and origin of the fermentation microbiota of mahewu, a Zimbabwean fermented cereal beverage.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

University of Alberta, Department of Agricultural, Food and Nutritional Science, Edmonton, Canada.

Mahewu is a fermented cereal beverage produced in Zimbabwe. This study determined the composition and origin of mahewu microbiota. The microbiota of mahewu samples consisted of 3 - 7 dominant strains of lactobacilli and two strains of yeasts. Read More

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http://dx.doi.org/10.1128/AEM.03130-18DOI Listing

The influence of plant species, tissue type and temperature on the capacity of Shigatoxigenic to colonise, grow and internalise into plants.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Cell and Molecular Sciences, The James Hutton Institute, Dundee, DD2 5DA, UK

Contamination of fresh produce with pathogenic including Shigatoxigenic (STEC), represents a serious risk to human health. Colonisation is governed by multiple bacterial and plant factors that can impact the probability and suitability of bacterial growth. Thus, we aimed to determine whether the growth potential of STEC for plants associated with foodborne outbreaks (two leafy vegetables and two sprouted seed species), is predictive for colonisation of living plants as assessed from growth kinetics and biofilm formation in plant extracts. Read More

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http://dx.doi.org/10.1128/AEM.00123-19DOI Listing
March 2019
1 Read

Next Generation of Tn-based single copy insertion elements for use in multi- and pandrug resistant strains of .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

The purpose of this study was to create single copy gene expression systems for use in genomic manipulations of multidrug resistant (MDR) and extreme drug resistant (XDR) clinical isolates of In this study, mini-Tn vectors with zeocin and apramycin selection markers were created by cloning and genes, respectively, enabling either inducible gene expression (pUC18T-mini-TnT-Zeo-LAC and pUC18T-mini-TnT-Apr-LAC) or expression from native or constitutive promoters (pUC18T-mini-TnT-Zeo and pUC18T-mini-TnT-Apr),. The selection markers of these plasmids are contained within an -cassette, which can be used to obtain unmarked mini-Tn insertions upon introduction of a source of Flp recombinase. To this end, site-specific excision vectors pFLP2A and pFLP2Z (containing apramycin and zeocin selection markers, respectively) were created in this study as an accessory to the mini-Tn vectors described above. Read More

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http://dx.doi.org/10.1128/AEM.00066-19DOI Listing

Determinants of Phage Host Range in Species.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, 30322

Bacteria in the genus are important targets for phage therapy due to their prevalence as pathogens and increasing antibiotic resistance. Here we review outer surface features and specific phage resistance mechanisms that define host range - the set of strains an individual phage can potentially infect. Phage infection goes through five distinct phases - attachment, uptake, biosynthesis, assembly and lysis. Read More

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http://dx.doi.org/10.1128/AEM.00209-19DOI Listing

Microcin PDI Inhibits Antibiotic-Resistant Strains of and Through a Mechanism of Membrane Disruption and Protection by Homotrimer Self-Immunity.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA

Microcin PDI (MccPDI), a class IIa microcin that is produced by strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic (EHEC) serotypes O157:H7 and O26. Herein we demonstrate that MccPDI can inhibit strains and isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion animals. Two exceptions out of 17 strains were identified. Read More

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http://dx.doi.org/10.1128/AEM.00371-19DOI Listing

Conserved Pyridoxal 5'-Phosphate Binding Protein YggS Impacts Amino Acid Metabolism through Pyridoxine 5'-Phosphate in .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furou-chou, Chikusa, Nagoya, Aichi 464-8601, Japan.

YggS (COG0325) is a member of the highly conserved pyridoxal 5'-phosphate (PLP)-binding protein family. Recent studies suggested a role for this protein family in the homeostasis of vitamin B and amino acids. The deletion or mutation of a member of this protein family causes pleiotropic effects in many organisms, and is causative of vitamin B-dependent epilepsy in humans. Read More

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http://dx.doi.org/10.1128/AEM.00430-19DOI Listing

Phototrophic lactate utilization by is stimulated by co-utilization with additional substrates.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Department of Biology, Indiana University, Bloomington, IN 47401

The phototrophic purple nonsulfur bacterium is known for its metabolic versatility and is of interest for various industrial and environmental applications. Despite decades of research on growth under diverse conditions, patterns of growth and carbon utilization with mixtures of carbon substrates remain largely unknown. readily utilizes most short chain organic acids but cannot readily use lactate as a sole carbon source. Read More

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http://dx.doi.org/10.1128/AEM.00048-19DOI Listing

Genomic characterization of candidate division LCP-89 reveals an atypical cell wall structure, microcompartment production, and dual respiratory and fermentative capacities.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Department of Microbiology and Molecular Genetics, Stillwater, OK 74074.

Recent experimental and bioinformatic advances enable the recovery of genomes belonging to yet-uncultured microbial lineages directly from environmental samples. Here, we report on the recovery and characterization of single amplified genomes (SAGs) and metagenome-assembled genomes (MAGs) representing candidate phylum LCP-89, previously defined based on 16S rRNA gene sequences. Analysis of LCP-89 genomes recovered from Zodletone spring, an anoxic spring in Oklahoma, predicts slow-growing, rod-shaped organisms. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00110-19
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http://dx.doi.org/10.1128/AEM.00110-19DOI Listing
March 2019
4 Reads

A Novel Antifungal Compound Z-705 Specifically Inhibits Protein Kinase C of Filamentous Fungi.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Sciences, Tohoku University, 468-1 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8572, Japan

The cell wall integrity signaling (CWIS) pathway is involved in fungal cell-wall biogenesis. This pathway is composed of sensor proteins, protein kinase C (PKC), and the MAP kinase pathway, and controls the transcription of many cell-wall related genes. PKC plays a pivotal role in this pathway; deficiency in PkcA in the model filamentous fungus and in MgPkc1 in the rice blast fungus is lethal. Read More

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http://dx.doi.org/10.1128/AEM.02923-18DOI Listing
March 2019
1 Read
3.668 Impact Factor

An Osmoregulatory Mechanism Operating through OmpR and LrhA Controls the Motile-Sessile Switch in the Plant Growth-Promoting Bacterium .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China

The adaptation to osmotic stress is crucial for bacterial growth and survival in changing environments. Although a large number of osmotic stress response genes have been identified in various bacterial species, how osmotic changes affect bacterial motility, biofilm formation and colonization of host niches remains largely unknown. In this study, we report that the LrhA regulator is an osmoregulated transcription factor that directly binds to the promoters of the , and operons and differentially regulates their expression, thus inhibiting motility and promoting exopolysaccharides (EPS) production, synthesis of osmoregulated periplasmic glucans (OPGs), biofilm formation and root colonization of the plant growth-promoting bacterium LTYR-11Z. Read More

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http://dx.doi.org/10.1128/AEM.00077-19DOI Listing
March 2019
3 Reads
3.668 Impact Factor

Characterization and genomic profiling of 2e, a newly isolated 1,3-propanediol-producing strain, revealed its adaptability to crude glycerol.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410008, Hunan, P.R. China.

Crude glycerol is largely generated as the main by-product of the biodiesel industry and is unprofitable for industrial application without costly purification. The direct bioconversion of crude glycerol into 1,3-propanediol (1,3-PDO) by microorganisms is a promising alternative for effective and economic utilization. In this study, 2e was newly isolated for the conversion of crude glycerol into 1,3-PDO. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00254-19
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http://dx.doi.org/10.1128/AEM.00254-19DOI Listing
March 2019
5 Reads

Comparative Genomic Analyses of Municipal Wastewater Isolates Detects a Clinically Unreported Salmonellosis Outbreak.

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Department of Civil and Environmental Engineering, University of Hawaii at Manoa, Honolulu, HI 96822

Municipal wastewater (MW) includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was monitored over a 54-week period. During this time, a serovar Paratyphi B var. Read More

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http://dx.doi.org/10.1128/AEM.00139-19DOI Listing

Differentiation of vegetative cells into spores: a kinetic model applied to .

Appl Environ Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Univ Brest, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, UMT ALTER'iX, F-29000 Quimper, France.

Spore-forming bacteria are natural contaminants of food raw materials and sporulation can occur in many environments from farm to fork. In order to characterize and to predict spore formation over time, we developed a model that describes both the kinetics of growth and the differentiation of vegetative cells into spores. The model is based on a classical growth model and enables to describe the kinetic of sporulation with the addition of three parameters specific of the sporulation. Read More

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http://dx.doi.org/10.1128/AEM.00322-19DOI Listing
March 2019
1 Read