33,280 results match your criteria Applied and Environmental Microbiology[Journal]


Erratum for Yang et al., " sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid".

Appl Environ Microbiol 2020 Jun 2;86(12). Epub 2020 Jun 2.

Department of Microbiology, Key Laboratory of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China

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http://dx.doi.org/10.1128/AEM.00957-20DOI Listing

CheY1 and CheY2 of ORS571 regulate chemotaxis and competitive colonization with the host plant.

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China

The genome of ORS571 encodes two chemotaxis response regulators-CheY1 and CheY2. is located in chemotaxis cluster (), while is located 37 kb upstream of the cluster. To determine the contributions of CheY1 and CheY2, we compared the wild-type (WT) and mutants in the free-living state and in symbiosis with the host Swim plate tests and capillary assays revealed that both CheY1 and CheY2 play a role in chemotaxis, with CheY2 having a more prominent role than CheY1. Read More

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http://dx.doi.org/10.1128/AEM.00599-20DOI Listing

Specific root exudates compounds sensed by dedicated chemoreceptors shape chemotaxis in the rhizosphere.

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Department of Biochemistry and Cellular & Molecular Biology, the University of Tennessee, Knoxville, USA

Plant roots shape the rhizosphere community by secreting compounds that recruit diverse bacteria. Colonization of various plant roots by the motile alphaproteobacterium s causes increased plant growth, root volume, and crop yield. Bacterial chemotaxis in this and other motile soil bacteria is critical for competitive colonization of the root surfaces. Read More

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http://dx.doi.org/10.1128/AEM.01026-20DOI Listing

Contribution of Complex I NADH dehydrogenase to respiratory energy coupling in glucose-grown cultures of .

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

The thermotolerant yeast (formerly ) is an industrially relevant production host that exhibits a fully respiratory sugar metabolism in aerobic batch cultures. NADH-derived electrons can enter its mitochondrial respiratory chain either via a proton-translocating Complex I NADH-dehydrogenase or via three putative alternative NADH dehydrogenases. This respiratory entry point affects the amount of ATP produced per NADH/O consumed and therefore impacts the maximum yield of biomass and/or cellular products from a given amount of substrate. Read More

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http://dx.doi.org/10.1128/AEM.00678-20DOI Listing

Nisin M is a bioengineered Nisin A variant that retains full induction capacity but has significantly reduced antimicrobial activity.

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

School of Microbiology, University College Cork, Cork, Ireland

Nisin A is a potent antimicrobial with potential as an alternative to traditional antibiotics, and a number of genetically modified variants have been created that target clinically relevant pathogens. In addition to antimicrobial activity, nisin auto-regulates its own production via a signal transduction pathway, a property that has been exploited in a protein expression system termed the Nisin Controlled Gene Expression (NICE) system. Although NICE has become one of the most popular protein expression systems, one drawback is that the inducer peptide, nisin A, also has inhibitory activity. Read More

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http://dx.doi.org/10.1128/AEM.00984-20DOI Listing

Prevalence of cefotaxime-resistant isolated from healthy cattle and sheep in Northern Spain: phenotypic and genome-based characterization of antimicrobial susceptibility.

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Animal Health Department, NEIKER - Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Bizkaia Science and Technology Park 812L, 48160 Derio, Bizkaia, Spain

In order to estimate herd-level prevalence of ESBL-/AmpC- and carbapenemase-producing commensal in ruminants in the Basque Country (Northern Spain), a cross-sectional survey was conducted in 2014-2016 in 300 herds using selective isolation. ESBL-/AmpC-producing were isolated in 32.9% of dairy cattle herds, 9. Read More

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http://dx.doi.org/10.1128/AEM.00742-20DOI Listing

Microbial degradation of pyridine: a complete pathway deciphered in sp. 68b.

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Lithuania.

Pyridine and its derivatives constitute majority of heterocyclic aromatic compounds that occur largely as a result of human activities and contribute to the environmental pollution. It is known, that they can be degraded by various bacteria in the environment, however, the degradation of unsubstituted pyridine has not yet been completely resolved. In this study we present data on the pyridine catabolic pathway in sp. Read More

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http://dx.doi.org/10.1128/AEM.00902-20DOI Listing

Quantitative proteome profiling reveals cellobiose-dependent protein processing and export pathways for the lignocellulolytic responses in .

Appl Environ Microbiol 2020 May 29. Epub 2020 May 29.

Biomass Energy Center for Arid and Semi-Arid Lands, Northwest A&F University, Yangling, Shaanxi 712100, PR China

Filamentous fungi are intensively used for producing industrial enzymes, including lignocellulases. Using insoluble cellulose to induce the production of lignocellulases causes some drawbacks, e.g. Read More

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http://dx.doi.org/10.1128/AEM.00653-20DOI Listing

-12 Exopolysaccharides Have Anti-Biofilm Activity Against .

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.

In the developing countries, is the most common enteric pathogen causing bacillary dysentery. And the biofilm formation of can cause the emergence of antibiotic-resistant strains and serious threat to food safety and human health. In this study, the effects of -12 exopolysaccharides (L-EPS) and exopolysaccharides (S-EPS) on the CMCC51574 biofilm formation were investigated. Read More

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http://dx.doi.org/10.1128/AEM.00694-20DOI Listing

Environmental surveillance complements the case-based AFP surveillance in the polio endgame strategy.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention; Shandong Center for Disease Control and Prevention, 16992 Jingshi Road, Jinan, Shandong, 250014, People's Republic of China

The Polio Endgame Strategy 2019-2023 has been developed. However, more effective and efficient surveillance activities should be conducted with the preparedness of emergence for vaccine-derived poliovirus (VDPV) or wild poliovirus (WPV). We reviewed the impact of the case-based acute flaccid paralysis (AFP) surveillance (1991-2018) and environmental surveillance (2011-2018) in polio eradication in Shandong province of China. Read More

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http://dx.doi.org/10.1128/AEM.00702-20DOI Listing

Identification of a global regulator and its involvement in the regulation network of rubber degradation in VH2.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany

A cAMP receptor protein (CRP) was detected as global regulator in VH2 and was supposed to participate in the network regulating poly(-1,4-isoprene) degradation as novel key regulator. CRP shares a sequence identity of 79 % with GlxR, a well-studied global regulator of Furthermore, CRP and GlxR have a common oligomerization state, similar binding motifs and thus most likely similar functions as global regulators. Size exclusion chromatography of purified CRP confirmed the existence as a homodimer with a native molecular weight of 44. Read More

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http://dx.doi.org/10.1128/AEM.00774-20DOI Listing

Microorganisms Move a Short Distance into an Almond Orchard From an Adjacent Upwind Poultry Operation.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Department of Food Science and Technology, University of California, Davis, One Shields Ave., Davis, California, 95616, USA

Over a 2-year period, drag swabs of orchard soil surface, and air, soil, and almond leaf samples were collected in an almond orchard adjacent to (35 m from first row of trees) and downwind from a poultry operation, and in two almond orchards (controls) that were surrounded by other orchards. Samples were evaluated for aerobic plate count, generic , other coliforms, presence of , bacterial community analysis through sequencing of the 16s rRNA gene, and amounts of dry solids (dust) on leaf surfaces on trees 0, 60, and 120 m into each orchard. was isolated from 41 of 206 (20%) and one of 207 (0. Read More

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http://dx.doi.org/10.1128/AEM.00573-20DOI Listing

An 1,4-α-glucosyltransferase defines a new maltodextrin catabolism scheme in .

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark

The maltooligosaccharide (MOS) utilization locus in NCFM, a model for human small-intestine lactobacilli, encodes three glycoside hydrolases (GHs): a putative maltogenic α-amylase of family 13 subfamily 20 (GH13_20), a maltose phosphorylase of GH65 (GH65) and a family 13 subfamily 31 member (GH13_31B), annotated as a 1,6-α-glucosidase. Here, we reveal that GH13_31B is a 1,4-α-glucosyltransferase that disproportionates MOS of degree of polymerization (DP) ≥2, with preference for maltotriose. Kinetic analyses of the three GHs encoded by the MOS locus, revealed that the substrate preference of GH13_31B towards maltotriose, complements the about 40-fold lower of GH13_20 towards this substrate, thereby enhancing the conversion of odd-numbered MOS to maltose. Read More

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http://dx.doi.org/10.1128/AEM.00661-20DOI Listing

The 5,6,7,8-tetrahydro-2-naphthoyl-CoA reductase reaction in the anaerobic degradation of naphthalene and identification of downstream metabolites.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Biofilm Centre, University of Duisburg-Essen, Essen, Germany

Anaerobic degradation of polycyclic aromatic hydrocarbons has been mostly investigated with naphthalene as a model compound. Naphthalene degradation by sulphate-reducing bacteria proceeds via carboxylation to 2-naphthoic acid, formation of a coenzyme A thioester and subsequent reduction to 5,6,7,8-tetrahydro-2-naphthoyl-CoA (THNCoA), which is further reduced to hexahydro-2-naphthoyl-CoA (HHNCoA) by tetrahydronaphthoyl-CoA reductase (THNCoA reductase), an enzyme similar to class I benzoyl-CoA reductases. When analysing THNCoA reductase assays with crude cell extracts and NADH as electron donor via LC-MS, scanning for putative metabolites, we could show that small amounts of the product of an HHNCoA hydratase are formed in the assays, but the downstream conversion by an NAD-dependent β-hydroxyacyl-CoA dehydrogenase was prevented by the excess of NADH present in those assays. Read More

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http://dx.doi.org/10.1128/AEM.00996-20DOI Listing

A sulfoglycolytic Entner-Doudoroff pathway in bv. SRDI565.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

School of Chemistry and Bio21 Molecular Science and Biotechnology Institute and University of Melbourne, Parkville, Victoria 3010, Australia

Rhizobia are nitrogen fixing bacteria that engage in symbiotic relationships with plant hosts but can also persist as free-living bacteria with the soil and rhizosphere. Here we show that free living SRDI565 can grow on the sulfosugar sulfoquinovose (SQ), or the related glycoside SQ-glycerol, using a sulfoglycolytic Entner-Doudoroff (sulfo-ED) pathway resulting in production of sulfolactate (SL) as the major metabolic end-product. Comparative proteomics supports the involvement of a sulfo-ED operon encoding an ABC transporter cassette, sulfo-ED enzymes and an SL exporter. Read More

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http://dx.doi.org/10.1128/AEM.00750-20DOI Listing

Transformation of the sex-manipulative endosymbiont and persisting hurdles for functional genetics studies.

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

Insects are frequently infected by bacterial symbionts that greatly affect their physiology and ecology. Most of these endosymbionts are however barely tractable outside of their native host, rendering functional genetics studies difficult or impossible. is a facultative bacterial endosymbiont of that manipulates its host reproduction by killing its male progeny at the embryonic stage. Read More

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http://dx.doi.org/10.1128/AEM.00835-20DOI Listing

SpoVG modulates cell aggregation by regulating expression and eDNA release in .

Appl Environ Microbiol 2020 May 22. Epub 2020 May 22.

Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China. Hefei, Anhui 230027, China

Biofilm formation is involved in numerous infections such as endocarditis, septic arthritis, osteomyelitis, and infections on in-dwelling medical devices. In these diseases, forms biofilms as cell aggregates interspersed in host matrix material. Here, we have observed that cell aggregation was significantly higher in the isogenic -deletion strain compared with that of the wild-type strain. Read More

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http://dx.doi.org/10.1128/AEM.00591-20DOI Listing

The sterol carrier hydroxypropyl--cyclodextrin enhances metabolism of phytosterols by .

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, P.R. China.

Androst-4-ene-3,17-dione (AD) and androst-1,4-diene-3,17-dione (ADD) are valuable steroid pharmaceutical intermediates obtained by soybean phytosterol biotransformation by Cyclodextrins (CDs) are generally believed as carriers for phytosterol delivery can improve the production of AD and ADD due to their effects on steroid solubilization and alteration in cell wall permeability for steroids. To better understand the mechanisms of CD promotion, we performed proteomic quantification of the effects of hydroxypropyl--CD on phytosterol metabolism in TCCC 11978 C2. Perturbations are observed in steroid catabolism and glucose metabolism by adding HP--CD in phytosterol bioconversion system. Read More

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http://dx.doi.org/10.1128/AEM.00441-20DOI Listing

Ethanol Metabolism Dynamics in Grown on Carbon Monoxide.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101 Qingdao, P. R. China

Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated that ethanol metabolism plays an important role in recycling NADH/NAD during autotrophic growth. Read More

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http://dx.doi.org/10.1128/AEM.00730-20DOI Listing

Ethanol and lysozyme immobilize phosphatidylserine on cell surface for evaluating apoptosis-like decay in activated sludge bacteria.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, P. R. China

Accurate determination of microbial viability can be crucial in microbe-dominated biosystems. However, the identification of metabolic decay in bacterial cells can be elaborate and difficult. We sought to identify apoptosis-like bacterial processes by using Annexin-V-FITC (AVF), a probe typically used to stain phosphatidylserine (PS) on exposed cell membranes. Read More

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http://dx.doi.org/10.1128/AEM.00345-20DOI Listing

Antimicrobial peptide GH12 prevents dental caries by regulating dental plaque microbiota.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

State Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Disease, Sichuan University, Chengdu, China

Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharides (EPS) synthesis of the biofilm and the three-species biofilm in the previous studies. However, the anticaries effects and microecological effects of GH12 remained to be investigated in the complex biofilm model and the animal caries model In the present study, GH12 at 64 mg/mL showed the most effective inhibition on lactic acid production, EPS synthesis, pH decline and biofilm integrity of the human dental plaque-derived multispecies biofilms , and GH12 at 64 mg/L was therefore chosen for subsequent assays and When treated with 64 mg/L GH12, the dental plaque-derived multispecies biofilms sampled from the healthy volunteers maintained the microbial diversity and showed a similar microbial community structure to the control group. Read More

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http://dx.doi.org/10.1128/AEM.00527-20DOI Listing

Insights into the function and horizontal transfer of isoproturon-degrading genes in a biobed system.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Agroécologie, AgroSup Dijon, INRAE, Univ Bourgogne, Univ Bourgogne Franche-Comté, Dijon, France

Biobeds, designed to minimize pesticide point source contamination, mainly rely on biodegradation processes. We studied the interactions of a biobed microbial community with the herbicide isoproturon (IPU) to explore the role of the gene, encoding the large subunit of an N-demethylase responsible for the initial demethylation of IPU via qPCR and RT-qPCR and the effect of IPU on the diversity of the total bacterial community and its active fraction through amplicon sequencing of DNA and RNA, respectively. We further investigated the localization and dispersal mechanism of in the biobed packing material by measuring the abundance of the plasmid pSH (harboring ) of the IPU-degrading sp. Read More

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http://dx.doi.org/10.1128/AEM.00474-20DOI Listing

Chromosome engineering to generate plasmid-free phenylalanine- and tyrosine-overproducing which can be applied in generation of aromatic compound-producing strains.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Osaka Research Institute of Industrial Science and Technology, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan.

Many phenylalanine- and tyrosine-producing strains have used plasmid-based overexpression of pathway genes. The resulting strains achieved high titer and yield of phenylalanine and tyrosine. Chromosomally-engineered, plasmid-free producers have shown lower titer and yield than plasmid-based strains, but the former are advantageous in terms of cultivation cost and public health/environmental risk. Read More

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http://dx.doi.org/10.1128/AEM.00525-20DOI Listing

Microbial dynamics in traditional and modern sour beer production.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Aas, Norway.

Traditional sour beers are produced by spontaneous fermentations involving numerous yeast and bacterial species. One of the traits that separates sour beers from ales and lagers is the high concentration of organic acids such as lactic acid and acetic acid, which results in reduced pH and increased acidic taste. Several challenges complicate production of sour beers through traditional methods. Read More

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http://dx.doi.org/10.1128/AEM.00566-20DOI Listing

The ABC transporter DerAB of mediates resistance against insect-derived defensins.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Institut für Mikrobiologie, Technische Universität Dresden, Dresden, Germany

Bce-like systems mediate resistance against antimicrobial peptides in Firmicutes bacteria. BL23 encodes an 'orphan' ABC transporter that, based on homology to BceAB-like systems, was proposed to contribute to antimicrobial peptide resistance. A mutant lacking the permease subunit was tested for sensitivity against a collection of peptides derived from bacteria, fungi, insects and humans. Read More

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http://dx.doi.org/10.1128/AEM.00818-20DOI Listing

Generation of Xylose-inducible promoter tools for species and their use in implicating a role for the Type II secretion system protein XcpQ in inhibition of corneal epithelial wound closure.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, USA

Tunable control of gene expression is an invaluable tool for biological experiments. In this study, we describe a new xylose-inducible promoter system and evaluate it in both and The promoter derived from the operon was incorporated into a broad host-range pBBR1-based plasmid and compared to the -derived promoter using as a reporter. GFP-fluorescence from the promoter was inducible in both species, but not in which may facilitate cloning of toxic genes using to generate plasmids. Read More

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http://dx.doi.org/10.1128/AEM.00250-20DOI Listing

In depth longitudinal study of ST9 isolates from meat processing industry: Resolving diversity and transmission patterns using WGS.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Nofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, Ås, Norway.

is a pathogen mostly associated with consumption of ready-to-eat foods and can cause severe disease and death. It can be introduced to food chains from raw materials, but often the contamination source is the food production environment where certain clones can persist for years. In the meat chain, ST9 is one of the most commonly encountered sequence types, and for effective source tracking, the divergence and spread of ST9 must be understood. Read More

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http://dx.doi.org/10.1128/AEM.00579-20DOI Listing

Genome-resolved metagenomics and detailed geochemical speciation analyses yield new insights into microbial mercury cycling in geothermal springs.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

School of Earth Sciences, The University of Melbourne, Parkville, VIC, Australia.

Geothermal systems emit substantial amounts of aqueous, gaseous and methylated mercury, but little is known about microbial influences on mercury speciation. Here we report results from genome-resolved metagenomics and mercury speciation analysis of acid warm springs in the Ngawha Geothermal Field (<55 °C, pH < 4.5), Northland Region, Aotearoa New Zealand. Read More

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http://dx.doi.org/10.1128/AEM.00176-20DOI Listing

Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Department of Biocatalysis, Institute of Catalysis and Petrochemistry, CSIC, C/Marie Curie 2, 28049, Madrid, Spain.

Ancestral sequence reconstruction and resurrection provides useful information for protein engineering, yet its alliance with directed evolution has been little explored. In this study we have resurrected several ancestral nodes of fungal laccases dating back ∼500-250 million years. Unlike modern laccases, the resurrected Mesozoic laccases were readily secreted by yeast, with similar kinetic parameters, a broader stability and distinct pH profiles of activity. Read More

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http://dx.doi.org/10.1128/AEM.00778-20DOI Listing

Interaction between GMP synthase and either human Glrx3 or Grx3/Grx4 converges in the regulation of Gcn2 pathway.

Appl Environ Microbiol 2020 May 15. Epub 2020 May 15.

Cell Signalling in Yeast unit, Department of Basic Medical Sciences, Institut de Recerca Biomèdica de Lleida (IRBLleida), University of Lleida, 25198, Lleida, Spain.

The human monothiol glutaredoxin Glrx3 (PICOT) is ubiquitously distributed in cytoplasm and nuclei in mammalian cells. Its overexpression has been associated to the development of several types of tumors whereas its deficiency might cause retardation in embryogenesis. Its exact biological role has not been well resolved, although a function as chaperone distributing iron/sulphur clusters is currently accepted. Read More

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http://dx.doi.org/10.1128/AEM.00221-20DOI Listing

Inactivation Kinetics and Membrane Potential of Pathogens in Soybean Curd Subjected to Pulsed Ohmic Heating Depending on Applied Voltage and Duty Ratio.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

The aim of this research was to investigate the efficacy of the duty ratio and applied voltage on the inactivation of pathogens in soybean curd by pulsed ohmic heating (POH). The heating rate of soybean curd increased rapidly as the applied voltage increased, although the duty ratio does not affect the temperature profile. We supported this result by verifying that the electrical conductivity increased with the applied voltage. Read More

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http://dx.doi.org/10.1128/AEM.00656-20DOI Listing

Histone-like nucleoid-structuring protein (H-NS) paralogue StpA activates the type I-E CRISPR-Cas system against natural transformation in .

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.

Working mechanisms of CRISPR-Cas systems have been intensively studied. However, far less is known about how they are regulated. The histone-like nucleoid-structuring protein H-NS binds the promoter of genes (P ) and suppresses the type I-E CRISPR-Cas system in Although H-NS paralogue StpA also binds P , its role in regulating the CRISPR-Cas system remains unidentified. Read More

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http://dx.doi.org/10.1128/AEM.00731-20DOI Listing

A sensitive magnetic arsenite-specific biosensor hosted in magnetotactic bacteria.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Aix Marseille Univ, CEA, CNRS, BIAM, UMR 7265, Saint Paul-Lez-Durance France

According to the World Health Organization, arsenic is the water contaminant that affects the largest number of people worldwide. To limit its impact on the population, cheap, quick and easy systems of detection are required. One promising solution could be the use of whole-cell biosensors, which have been extensively studied and could meet all these criteria even though they often lack sensitivity. Read More

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http://dx.doi.org/10.1128/AEM.00803-20DOI Listing

Changes in the microbiome of mariculture feed organisms after treatment with a potentially probiotic strain of .

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads Bldg. 221, DK-2800 Kgs. Lyngby.

The genus has been explored as probiotics in mariculture as a sustainable strategy for prevention of bacterial infections. Its antagonistic effect against common fish pathogens is predominantly due to the production of the antibacterial compound tropodithietic acid (TDA) and TDA-producing strains have repeatedly been isolated from mariculture environments. Despite many trials targeting pathogens, little is known about its impact on host-associated microbiomes in mariculture. Read More

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http://dx.doi.org/10.1128/AEM.00499-20DOI Listing

A systems analysis of short and long-term transcriptomic response to biocides.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Genome Center, University of California, Davis, CA, USA.

The mechanisms of bacterial response to biocides are poorly understood, despite their broad application. To identify the genetic basis and pathways implicated in the biocide stress response, we exposed populations to ten ubiquitous biocides. By comparing the transcriptional responses between short (30min) and long-term (8 to 12h) exposure to biocide stress, we established the common gene and pathways clusters that are implicated in general and biocide-specific stress response. Read More

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http://dx.doi.org/10.1128/AEM.00708-20DOI Listing

Several species produce ergot alkaloids in a conditionally specific manner.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

West Virginia University, Division of Plant and Soil Sciences, Morgantown, WV 26506-6108

Genomic sequence data indicate certain fungi in the genus have the capacity to produce lysergic acid-derived ergot alkaloids, but accumulation of ergot alkaloids in these fungi has not been demonstrated previously. We assayed several species grown under different conditions for accumulation of ergot alkaloids. Isolates of and accumulated the lysergic acid amides lysergic acid α-hydroxyethyl amide, ergine, and ergonovine on sucrose-yeast extract agar but not on two other tested media. Read More

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http://dx.doi.org/10.1128/AEM.00373-20DOI Listing

Contrasting patterns in diversity and community assembly of root-associated bacterial communities from different seasons.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.

The common reed (), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e. Read More

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http://dx.doi.org/10.1128/AEM.00379-20DOI Listing

Can abundance of sensory proteins distinguish between pathogenic and non-pathogenic bacteria?

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Bio-Sciences R&D Division, TCS Innovation Labs, Tata Consultancy Services Limited, Pune, India.

Signal Transduction Systems are essential for microorganisms to respond to their ever changing environment. It can be distinguished into one-component systems, two-component system, and extracytoplasmic-function σ factors. Abundances of a few signal-transducing proteins, termed herein as Sensory Proteins (SPs), have previously been reported to be correlated to the genome size and the ecological niche of the certain gram-positive bacteria. Read More

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http://dx.doi.org/10.1128/AEM.00478-20DOI Listing

A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in DSM 792.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Biotechnology Department, IFP Energies nouvelles, Rueil-Malmaison, France.

Although is the model organism for the study of Acetone Butanol Ethanol (ABE) fermentation, its characterization has long been impeded by the lack of efficient genome editing tools. In particular, the contribution of alcohol dehydrogenases to solventogenesis in this bacteria has mostly been studied with the generation of single gene deletion strains. In this study, the three butanol dehydrogenases encoding genes located on the chromosome of the DSM 792 reference strain were deleted iteratively by using a recently developed CRISPR-Cas9 tool improved by using an anti-CRISPR protein encoding gene Although the literature has previously shown that inactivation of either , or had only moderate effects on the strain, this study shows that clean deletion of both and strongly impaired solvent production, and that a triple mutant ΔΔΔ was even more affected. Read More

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http://dx.doi.org/10.1128/AEM.00408-20DOI Listing

Deglycosylation of isoflavone -glucoside puerarin by combination of two recombinant bacterial enzymes and 3-oxo-glucose.

Appl Environ Microbiol 2020 May 8. Epub 2020 May 8.

Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science; 3500-3 Minamitamagaki, Suzuka, Mie, 513-8670, Japan

A human intestinal bacterium strain PUE related to species can metabolize the isoflavone -glucoside puerarin (daidzein 8--glucoside) to daidzein and glucose. We previously reported that 3"-oxo-puerarin is an essential reaction intermediate in enzymatic puerarin degradation and characterized a bacterial enzyme of DgpB-C complex which cleaved the -glycosidic bond in 3"-oxo-puerarin. However, the exact enzyme catalyzing the oxidation of C-3" hydroxyl in puerarin has not been identified. Read More

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http://dx.doi.org/10.1128/AEM.00607-20DOI Listing

A Framework for the Selection of Plant Growth-Promoting Rhizobacteria Based on Bacterial Competence Mechanisms.

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Bioproducts Department, Tibaitatá, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Mosquera, Colombia.

The use of plant growth-promoting rhizobacteria (PGPR) is increasingly meaningful for the development of more environmentally friendly agricultural practices. However, often the PGPR strains selected in the laboratory fail to confer the expected beneficial effects when evaluated in plant experiments. Insufficient rhizosphere colonization is pointed out as one of the causes. Read More

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http://dx.doi.org/10.1128/AEM.00760-20DOI Listing

Sea cucumber intestinal regeneration reveals deterministic assembly of the gut microbiome.

Authors:
Brooke L Weigel

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Committee on Evolutionary Biology and Department of Ecology and Evolution, University of Chicago, Chicago, IL, USA

The gut microbiome has far-reaching effects on host organism health, thus understanding the processes that underlie microbial community assembly in the developing gut is a current research priority. Here, a holothurian (a.k. Read More

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http://dx.doi.org/10.1128/AEM.00489-20DOI Listing

Identification of CFT073 fitness factors during urinary tract infection using an ordered transposon library.

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Department of Microbiology and Immunology, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109

Urinary tract infections (UTI), the second most diagnosed infectious disease worldwide, are caused primarily by uropathogenic (UPEC), placing a significant financial burden on the healthcare system. High-throughput transposon mutagenesis combined with genome-targeted sequencing is a powerful technique to interrogate genomes for fitness genes. Genome-wide analysis of requires random libraries of at least 50,000 mutants to achieve 99. Read More

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http://dx.doi.org/10.1128/AEM.00691-20DOI Listing

A novel toxin-antitoxin module SlvT-SlvA regulates megaplasmid stability and incites solvent tolerance in S12.

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Institute of Biology Leiden, Leiden University, The Netherlands

S12 is highly tolerant towards organic solvents in saturating concentrations, rendering this microorganism suitable for the industrial production of various aromatic compounds. Previous studies revealed that S12 contains a single-copy 583 kbp megaplasmid pTTS12. pTTS12 encodes several important operons and gene clusters facilitating S12 to survive and grow in the presence of toxic compounds or other environmental stresses. Read More

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http://dx.doi.org/10.1128/AEM.00686-20DOI Listing

Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species and .

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Institute of Sciences of Food Production, CNR, Bari, Italy.

and are common maize pathogens that produce mycotoxins and cause plant disease. The ability of these species to produce beauvericin and fumonisin mycotoxins is not settled, as reports of toxin production are not concordant. Our objective was to clarify this situation by determining both chemotypes and genotypes for strains from both species. Read More

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http://dx.doi.org/10.1128/AEM.00133-20DOI Listing

Glycotyping and specific separation of with a novel bacteriophage protein toolkit.

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland

The Gram-positive pathogen can be subdivided into at least 12 different serovars, based on differential expression of a set of somatic and flagellar antigens. Of note, strains belonging to serovars 1/2a, 1/2b and 4b cause the vast majority of foodborne listeriosis cases and outbreaks. The standard protocol for serovar determination involves an agglutination method using a set of sera containing cell surface-recognizing antibodies. Read More

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http://dx.doi.org/10.1128/AEM.00612-20DOI Listing

Antibiofilm Activity of Small Molecules Produced by against .

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Department of Microbial Biotechnology, Panjab University, Chandigarh - 160014, India

We read the article by Glatthardt (1) with great interest.…. Read More

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http://dx.doi.org/10.1128/AEM.00627-20DOI Listing

The effect of spring water geochemistry on copper proteins in Tengchong hot springs, China.

Appl Environ Microbiol 2020 May 1. Epub 2020 May 1.

Department of Biological Sciences, University of Alaska-Anchorage, Anchorage, AK 99508, USA.

Copper (Cu) is an essential trace metal cofactor for a variety of proteins, however, excess Cu is toxic to most organisms. Cu homeostasis is maintained by a complex machinery of Cu binding proteins that control the uptake, transport, sequestration, and efflux of Cu ions. Despite the importance of Cu-binding proteins in electron transfer, substrate oxidation, superoxide dismutation, and denitrification, little information exists about microbial Cu utilization in extreme environments, where the geochemical conditions may affect Cu bioavailability. Read More

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http://dx.doi.org/10.1128/AEM.00581-20DOI Listing