1,192,675 results match your criteria Applied Ocean Research[Journal]

gene knockout prediction using a hybrid of Bat algorithm and minimization of metabolic adjustment.

J Integr Bioinform 2021 Aug 3. Epub 2021 Aug 3.

Faculty of Computing (FKOM), College of Computing and Applied Sciences, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.

Microorganisms commonly produce many high-demand industrial products like fuels, food, vitamins, and other chemicals. Microbial strains are the strains of microorganisms, which can be optimized to improve their technological properties through metabolic engineering. Metabolic engineering is the process of overcoming cellular regulation in order to achieve a desired product or to generate a new product that the host cells do not usually need to produce. Read More

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Modified Mannan for 3D Bioprinting: A potential novel bioink for tissue engineering.

Biomed Mater 2021 Aug 4. Epub 2021 Aug 4.

College of Material Science and Engineering, Hunan University, Lushan Nan Lu, Yuelu District, Changsha City, Hunan Province, China, Changsha, Hunan, 410082, CHINA.

3D bioprinting technology displays many advantages for tissue engineering applications, but its utilization is limited by very few bioinks available for biofabrication. In this study, a novel type of bioink, which includes three methacryloyl modified mannans, was introduced to 3D bioprinting for tissue engineering applications. Yeast mannan (YM) was modified by reacting with methacrylate anhydride (MA) at different concentrations, and three YM derived bioinks were obtained, which were termed as YM-MA-1, YM-MA-2 and YM-MA-3 and were distinguished with different adjusted methacrylation degrees. Read More

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Tunable electronic properties and enhanced ferromagnetism in Cr2Ge2Te6 monolayer by strain engineering.

Nanotechnology 2021 Aug 4. Epub 2021 Aug 4.

Southeast University, SEU-FEI Nano-Pico Center, Key Laboratory of MEMS of Ministry of Education, Southeast University, Nanjing, China, Nanjing, 210096, CHINA.

Recently, as a new representative of Heisenberg's two-dimensional (2D) ferromagnetic materials, 2D Cr2Ge2Te6 (CGT), has attracted much attention due to its intrinsic ferromagnetism. Unfortunately, the Curie temperature (TC) of CGT monolayer is only 22K, which greatly hampers the development of the applications based on the CGT materials. Herein, by means of density functional theory computations, we explored the electronic and magnetic properties of Cr2Ge2Te6 monolayer under the applied strain. Read More

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Metabolism-driven post-translational modifications of H3K9 in early bovine embryos.

Reproduction 2021 Aug 4;162(3):181-191. Epub 2021 Aug 4.

Laboratory of Embryonic Metabolism and Epigenetics, Center of Natural Sciences and Humanities, Universidade Federal do ABC, Santo André, São Paulo, Brazil.

Metabolic and molecular profiles were reported as different for bovine embryos with distinct kinetics during the first cleavages. In this study, we used this same developmental model (fast vs slow) to determine if the relationship between metabolism and developmental kinetics affects the levels of acetylation or tri-methylation at histone H3 lysine 9 (H3K9ac and H3K9me3, respectively). Fast and slow developing embryos presented different levels of H3K9ac and H3K9me3 from the earliest stages of development (40 and 96 hpi) and up to the blastocyst stage. Read More

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Toxic effects of substituted p-benzoquinones and hydroquinones in in vitro bioassays are altered by reactions with the cell assay medium.

Water Res 2021 Jul 7;202:117415. Epub 2021 Jul 7.

Eawag, Swiss Federal Institute of Aquatic Science and Technology, Duebendorf CH-8600, Switzerland; Civil and Environmental Engineering (ENAC), School of Architecture, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland. Electronic address:

Substituted para-benzoquinones and hydroquinones are ubiquitous transformation products that arise during oxidative water treatment of phenolic precursors, for example through ozonation or chlorination. The benzoquinone structural motive is associated with mutagenicity and carcinogenicity, and also with induction of the oxidative stress response through the Nrf2 pathway. For either endpoint, toxicological data for differently substituted compounds are scarce. Read More

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Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts.

J Chromatogr A 2021 Jul 24;1653:462380. Epub 2021 Jul 24.

University of Defence, Faculty of Military Health Sciences, Department of Molecular Pathology and Biology, Trebešská 1575, 50001 Hradec Králové, Czech Republic.

Lipidomic samples are complex mixtures of structurally different species of a wide range of concentrations providing challenges in their characterization. In this work, we present a proof of concept for the application of a simple microgradient liquid chromatography device on the detailed analysis of lipid classes. Our lipidomic analysis is based on a lipid class microgradient fractionation of a total lipid extract using an in-house-prepared hydrophilic interaction liquid chromatography microcolumn followed by RP-LC/MS of the collected lipid class fractions. Read More

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Analysis of eight bile acids in urine of gastric cancer patients based on covalent organic framework enrichment coupled with liquid chromatography-tandem mass spectrometry.

J Chromatogr A 2021 Jul 22;1653:462422. Epub 2021 Jul 22.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, China. Electronic address:

Gastric carcinoma is one of the most common and deadly forms of cancer. Early detection is critical for successful treatment of gastric cancer, and examination of BAs in urine may provide a critical diagnostic tool for identifying gastric cancer at stages when it can still be cured. Bile acids (BAs) are a crucial toxic factor correlated with the injury of gastric mucosa and as such, quantifying the amount of BA in patient's urine could provide a new means to quickly and non-invasively identify the presence of gastric cancer in the early stages. Read More

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Characterization of dextran particle size: How frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) coupled online with dynamic light scattering (DLS) leads to enhanced size distribution.

J Chromatogr A 2021 Jul 24;1653:462404. Epub 2021 Jul 24.

Normandie University, UNIROUEN, National Institute of Applied Sciences of Rouen, CNRS, PBS, UMR6270, Rouen 76000, France. Electronic address:

Accurate determinations of particle size and particle size distribution (PSD) are essential to achieve the clinical translation of medical nanoparticles (NPs). Herein, dextran-based NPs produced via a water-in-oil emulsification/crosslinking process and developed as nanomedicines were studied. NPs were first characterized using traditional batch-mode techniques as dynamic light scattering (DLS) and laser diffraction. Read More

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Separation of vigabatrin enantiomers using mixed-mode chromatography and its application to determine the vigabatrin enantiomer levels in rat plasma.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jul 27;1179:122866. Epub 2021 Jul 27.

Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan. Electronic address:

Mixed-mode chromatography-comprising a mixed phase with reversed and ionic phases, enabling hydrophobic and ion-exchange interactions simultaneously-was applied to identify vigabatrin enantiomers by HPLC with pre-column fluorescence derivatization with 2,5-dioxopyrrolidin-1-yl (4-(((2-nitrophenyl)sulfonyl)oxy)-6-(3-oxomorpholino)quinoline-2-carbonyl)prolinate (Ns-MOK-(S)-Pro-OSu). The MOK-(S)-Pro-vigabatrin enantiomers were efficiently separated within 12 min (total analysis time per sample: 28 min, including washing and equilibrium time for the column). The mobile phase was HO/CHOH/10 mM ammonium formate (pH 2. Read More

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Addressing the punitive parent mode in schema therapy for borderline personality disorder: Short-term effects of the empty chair technique as compared to cognitive challenging.

J Behav Ther Exp Psychiatry 2021 Jul 22;73:101678. Epub 2021 Jul 22.

Department of Clinical Psychology, University of Amsterdam, Amsterdam, the Netherlands.

Background And Objectives: In Schema Therapy (ST) for Borderline Personality Disorder (BPD) patients the empty chair technique (EC) is often used to diminish the 'punitive parent mode' (PP). The present study is a first attempt to assess whether EC is more effective in reducing the PP than a standard Cognitive Behavioral Therapy technique (CT).

Methods: We utilized a counterbalanced, crossover design comparing one EC session to one CT session in twenty patients with a primary BPD diagnosis who had started ST. Read More

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Coronavirus Disease Analysis using Chest X-ray Images and a Novel Deep Convolutional Neural Network.

Photodiagnosis Photodyn Ther 2021 Aug 1:102473. Epub 2021 Aug 1.

Pattern Recognition Lab, Department of Computer & Information Sciences, Pakistan Institute of Engineering & Applied Sciences, Nilore, Islamabad 45650, Pakistan; PIEAS Artificial Intelligence Center (PAIC), Pakistan Institute of Engineering & Applied Sciences, Nilore, Islamabad 45650, Pakistan; Center for Mathematical Sciences, Pakistan Institute of Engineering & & Applied Sciences, Nilore, Islamabad 45650, Pakistan. Electronic address:

Background: The recent emergence of a highly infectious and contagious respiratory viral disease known as COVID-19 has vastly impacted human lives and overloaded the health care system. Therefore, it is crucial to develop a fast and accurate diagnostic system for the timely identification of COVID-19 infected patients and thus to control its spread.

Methods: This work proposes a new deep CNN based technique for COVID-19 classification in X-ray images. Read More

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Manifesto for an ECNP Neuromodulation Thematic Working Group (TWG): Non-invasive brain stimulation as a new Super-subspecialty.

Eur Neuropsychopharmacol 2021 Aug 1;52:72-83. Epub 2021 Aug 1.

Sheba Medical Center at Tel Hashomer, Israel, Sackler Faculty of Medicine, Tel Aviv.

Non-Invasive Brain Stimulation (NIBS) techniques and in particular, repetitive Transcranial Magnetic Stimulation (rTMS), are developing beyond mere clinical application. Although originally purposed for the treatment of resistant neuropsychiatric disorders, NIBS is also contributing to a deeper understanding of psychiatric disorders. rTMS is also changing the model of the disorder itself, from "mental" to one of neural connectivity. Read More

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Development and preliminary validation of the Adolescent Food Parenting Questionnaire: Parent and adolescent version.

Appetite 2021 Aug 1:105618. Epub 2021 Aug 1.

Behavioural Science Institute, Radboud University, Nijmegen, the Netherlands. Electronic address:

Suitable instruments for measuring Food Parenting Practices (FPP) among adolescents and their parents that also measure the perception of adolescents about their parent's FPP are rare. The current study describes the development and preliminary testing of a short 16-item Adolescent Food Parenting Questionnaire (AFPQ) for parents (AFPQ-p) and adolescents (AFPQ-a) that may enable future large-scale research on potentially eminent parent-child FPP discrepancy. Participants included 381 parents (73. Read More

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AOP and IATA applied to ocular surface toxicity.

Regul Toxicol Pharmacol 2021 Aug 1:105021. Epub 2021 Aug 1.

Sorbonne Université, INSERM, CNRS, IHU FOReSight, Institut de la Vision, 75012 Paris, France; Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, INSERM-DGOS CIC 1423, IHU FOReSight, 75012, France; Université de Paris, Faculté de Pharmacie de Paris, 75006 Paris, France. Electronic address:

Until now, the Draize test on rabbits has been the only test performed to anticipate ocular toxicity of pharmaceutical compounds, mainly irritation. The OECD is urging the scientific community to develop and validate alternative methods to reduce the need for animal testing. Since the models and tests used cannot reflect the entire biologic response, it is necessary to combine them into integrated approaches to testing and assessment (IATA) to obtain robust data. Read More

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The Modernization of Radiation Therapy Services in Cambodia: A Model of International Collaboration.

Int J Radiat Oncol Biol Phys 2021 Sep;111(1):14-22

Collaboration for Cancer Outcomes, Research and Evaluation (CCORE), Ingham Institute for Applied Medical Research, UNSW Sydney, Liverpool, Australia; Liverpool and Macarthur Cancer Therapy Centres, Western Sydney University, Campbelltown, Australia; School of Public Health, Faculty of Medicine and Health, University of Sydney, Camperdown, Australia. Electronic address:

Purpose: Cambodia is a Southeast Asian low-middle-income country with a population of >15 million. In 2020, Cambodia was estimated to have 18,375 new diagnoses of cancer and 12,638 deaths attributable to cancer. Cambodia was estimated to have a deficit of 16 megavoltage machines in 2012. Read More

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September 2021

Keeping Kids Kool-Influence of Induction, Advantage of Adaptation, Curtailment of Complications.

Int J Radiat Oncol Biol Phys 2021 Sep;111(1):12-13

Department of Radiation Medicine and Applied Sciences, University of California San Diego, La Jolla, California; Center for Precision Radiation Medicine, La Jolla, California. Electronic address:

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September 2021

Revealing protein-protein interactions at the transcriptome scale by sequencing.

Mol Cell 2021 Jul 27. Epub 2021 Jul 27.

Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address:

We describe PROPER-seq (protein-protein interaction sequencing) to map protein-protein interactions (PPIs) en masse. PROPER-seq first converts transcriptomes of input cells into RNA-barcoded protein libraries, in which all interacting protein pairs are captured through nucleotide barcode ligation, recorded as chimeric DNA sequences, and decoded at once by sequencing and mapping. We applied PROPER-seq to human embryonic kidney cells, T lymphocytes, and endothelial cells and identified 210,518 human PPIs (collected in the PROPER v. Read More

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Flavan-based phytoconstituents inhibit Mpro, a SARS-COV-2 molecular target, .

J Biomol Struct Dyn 2021 Aug 4:1-15. Epub 2021 Aug 4.

Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan.

A well-validated approach can provide promising drug candidates for the treatment of the ongoing CoVID19 pandemic. In this study, we have screened 32 phytochemical constituents (PCCs) with Mpro binding site (PDB:6W63) based on which we identified three possible candidates that are likely to be effective against CoVID19-viz., licoleafol (binding energy: -8. Read More

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Determination of quinclorac by adsorptive stripping voltammetry in rice samples without sample pretreatment.

J Environ Sci Health B 2021 Aug 4:1-10. Epub 2021 Aug 4.

Applied Electroanalytical Group, Department of Chemistry, Federal University of Viçosa, Viçosa, Brazil.

A novel voltammetric method with practically no sample pretreatment was developed for determination of Quinclorac (QNC) in rice samples by using a working Carbon Paste Electrode (CPE) modified with ionic liquid, with deposition potential (E) of -1.43 V for 30 s in NaOH 0.01 mol L. Read More

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Does vitamin B12 deficiency explain psychiatric symptoms in recreational nitrous oxide users? A narrative review.

Clin Toxicol (Phila) 2021 Aug 4:1-9. Epub 2021 Aug 4.

Department of Medical Physiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Recreational use of nitrous oxide (NO) is associated with many side effects, of which neurological complications are most common. Nitrous oxide abuse is also associated with psychiatric symptoms, but these have received less attention so far. Vitamin B12 deficiency may play a role in the development of these psychiatric symptoms. Read More

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Dietary Inflammatory Index in Relation to Psoriasis Risk, Cardiovascular Risk Factors and Clinical Outcomes; A Result from Case-Control Study in Psoriasis Patients.

Appl Physiol Nutr Metab 2021 Aug 4. Epub 2021 Aug 4.

Thapar Institute of Engineering & Technology, Department of Biotechnology, Delhi , India;

Psoriasis is an inflammatory skin disease. Despite the understanding of the disease pathogenesis, the link between diet-induced inflammation and the risk of psoriasis remains underexplored. Therefore, we have examined the capability of literature-derived Energy-adjusted Dietary Inflammatory Index (E-DII) as a predictive tool for inflammation, incidence, and severity of psoriasis (as indexed by The Psoriasis Area Severity Index (PASI)). Read More

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Native Separation and Metallation Analysis of SOD1 Protein from the Human Central Nervous System: a Methodological Workflow.

Anal Chem 2021 Aug 4. Epub 2021 Aug 4.

Univ. Bordeaux, CNRS, CENBG, UMR-5797, F-33170 Gradignan, France.

Studies of the metal content of metalloproteins in tissues from the human central nervous system (CNS) can be compromised by preparative techniques which alter levels of, or interactions between, metals and the protein of interest within a complex mixture. We developed a methodological workflow combining size exclusion chromatography, native isoelectric focusing, and either proton or synchrotron X-ray fluorescence within electrophoresis gels to analyze the endogenous metal content of copper-zinc superoxide dismutase (SOD1) purified from minimal amounts (<20 mg) of post-mortem human brain and spinal cord tissue. Abnormal metallation and aggregation of SOD1 are suspected to play a role in amyotrophic lateral sclerosis and Parkinson's disease, but data describing SOD1 metal occupancy in human tissues have not previously been reported. Read More

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Comparison of Periodontally Compromised Splinted Teeth and Implant Supported Fixed Partial Denture: A Three-Dimensional Finite Element Analysis on Bone Response.

J Long Term Eff Med Implants 2021 ;31(2):1-8

DDS, Private Practice, Tehran, Iran.

Introduction - This study aimed to compare the amount and pattern of stress and strain distributed around periodontally compromised splinted teeth and the two-implant abutments supported six-unit fixed partial denture (FPD) using finite element analysis (FEA). Methods and Materials - Six mandibular anterior teeth of a dental model were scanned and the scans were transferred to 3D CAD design and finite element software. Jaw bone was also designed and the teeth were splinted by fiber-reinforced composite (FRC) band. Read More

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January 2021

Biotechnical System of Differential Diagnostics of Serous and Purulent Pyelonephritis in Pregnant Women Based on Fuzzy Logic for Decision-Making.

Crit Rev Biomed Eng 2021 ;49(1):67-75

Faculty of Biomedical Engineering, Southwest State Technical University, Kursk, Russia.

Urologists and nephrologists attribute pyelonephritis of pregnant women to the category of complicated upper urinary tract infections that threaten the development of a severe purulent-septic process. The frequency of pyelonephritis in pregnant women ranges from 12.2 to 33. Read More

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January 2021

The pesticide residue analysis in commodities with high content of chlorophyll based on the quick, easy, cheap, effective, rugged and safe method: A review.

J Sep Sci 2021 Aug 4. Epub 2021 Aug 4.

Institute of Plant Protection - National Research Institute Branch Sośnicowice, Gliwicka 29 Street, Sośnicowice, 44-153, Poland.

In multiresidue analysis, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) is one of the most popular techniques routinely used by researchers during pesticide analysis of food and vegetables samples. Originally, the QuEChERS method was developed for analysis of pesticide residues from fruits and vegetables, but rapidly gained popularity in the extraction of analytes from different matrices. This analytical approach shows several advantages over traditional extraction techniques: it requires lower sample and solvent amounts while shortening the time of sample preparation. Read More

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Identification of Rab family genes and functional analyses of LmRab5 and LmRab11A in the development and RNA interference of Locusta migratoria.

Insect Sci 2021 Aug 4. Epub 2021 Aug 4.

Institute of Applied Biology, Shanxi University, Taiyuan, China.

Rab proteins constitute the largest family of small GTPases, which play pivotal roles in intracellular membrane trafficking in all eukaryotes. A number of Rab genes have been identified in eukaryotes; however, very little information about these genes has been reported in insects. In the current study, for the first time we identified and characterized 27 Rab family genes from Locusta migratoria. Read More

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Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions.

J Clin Lab Anal 2021 Aug 4:e23939. Epub 2021 Aug 4.

National Research Center for Hematology of the Ministry of Health of the Russian Federation, Moscow, Russia.

Aim: To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions.

Methods: We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Read More

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