21,115 results match your criteria Applied Immunohistochemistry & Molecular Morphology[Journal]


Huangkui capsule alleviates renal tubular epithelial-mesenchymal transition in diabetic nephropathy via inhibiting NLRP3 inflammasome activation and TLR4/NF-κB signaling.

Phytomedicine 2018 Dec 17;57:203-214. Epub 2018 Dec 17.

Institute of Huangkui, Suzhong Pharmaceutical Group Co., Ltd., 1 Suzhong Road, Taizhou, Jiangsu 225500, China. Electronic address:

Background: Huangkui capsule (HKC), an anti-inflammatory Chinese modern patent medicine, has been now widely applied to the clinical therapy of diabetic nephropathy (DN). However, the overall therapeutic mechanisms in vivo are still unclear. Renal tubular epithelial-to-mesenchymal transition (EMT) is one of the major pathogenesis of renal interstitial fibrosis in DN. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.12.021DOI Listing
December 2018

Protective effect of Xin-Ji-Er-Kang on cardiovascular remodeling in high salt-induced hypertensive mice.

Exp Ther Med 2019 Mar 17;17(3):1551-1562. Epub 2018 Dec 17.

Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei, Anhui 230032, P.R. China.

The aim of the present study was to investigate the effects of Xin-Ji-Er-Kang (XJEK) on high salt-induced hypertensive mice. Mice with high-salt diet-induced hypertension were divided into four groups: Control (standard diet alone for 8 weeks), model (diet containing 8% NaCl for 8 weeks and intragastric administration of distilled water for the last 4 weeks), XJEK + high-salt-treated (diet containing 8% NaCl for 8 weeks and intragastric administration of XJEK for the last 4 weeks) and irbesartan + high-salt-treated (diet containing 8% NaCl for 8 weeks with intragastric administration of irbesartan for the last 4 weeks). The hemodynamic index and cardiac pathological changes in the hypertensive mice were then examined. Read More

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http://dx.doi.org/10.3892/etm.2018.7105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364186PMC

Screw Track Osteolysis in the Cementless Total Knee Replacement Design.

J Arthroplasty 2019 Jan 6. Epub 2019 Jan 6.

Department of Orthopaedic Surgery, Otto-von-Guericke University, Magdeburg, Germany.

Background: There is a paucity of reports on osteolysis associated with tibial screw fixation in cementless total knee arthroplasty (TKA), and the pathophysiology is not clear. This study aimed to describe the pathology related to screw track osteolysis around the tibia in cementless TKA.

Methods: The study cohort comprised 100 revised cementless TKAs with tibial screw fixation. Read More

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http://dx.doi.org/10.1016/j.arth.2018.12.040DOI Listing
January 2019

Mechanosensitive meningeal nociception via Piezo channels: Implications for pulsatile pain in migraine?

Neuropharmacology 2019 Feb 13;149:113-123. Epub 2019 Feb 13.

Department of Neurobiology, A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, 70211, Finland; Laboratory of Neurobiology, Kazan Federal University, Kazan, 420008, Russia. Electronic address:

Background: Recent discovery of mechanosensitive Piezo receptors in trigeminal ganglia suggested the novel molecular candidate for generation of migraine pain. However, the contribution of Piezo channels in migraine pathology was not tested yet. Therefore, in this study, we explored a potential involvement of Piezo channels in peripheral trigeminal nociception implicated in generation of migraine pain. Read More

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http://dx.doi.org/10.1016/j.neuropharm.2019.02.015DOI Listing
February 2019
1 Read

Ornithine decarboxylase antizyme inhibitor 2 (AZIN2) is a signature of secretory phenotype and independent predictor of adverse prognosis in colorectal cancer.

PLoS One 2019 15;14(2):e0211564. Epub 2019 Feb 15.

Department of Pathology, Haartman Institute, University of Helsinki and HUSLAB, Helsinki, Finland.

Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine synthesis. The two ODC antizyme inhibitors (AZIN1) and (AZIN2) are regulators of the catalytic activity of ODC. While AZIN1 is a regulator of cell proliferation, AZIN2 is involved in intracellular vesicle transport and secretion. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211564PLOS
February 2019

Knockdown of PHF5A Inhibits Migration and Invasion of HCC Cells via Downregulating NF-B Signaling.

Biomed Res Int 2019 15;2019:1621854. Epub 2019 Jan 15.

Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute, Sun Yat-sen University and Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China.

Background: Inflammation is the major risk factor for the progression of hepatocellular carcinoma (HCC), and the nuclear factor-B (NF-B) signaling plays the central role in the inflammation process. However, the activated mechanism of NF-B signaling in HCC is unclear.

Methods: The expression of PHF5A is examined by qPCR, western blotting, and immunohistochemistry (IHC) assay. Read More

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http://dx.doi.org/10.1155/2019/1621854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350539PMC
January 2019

Molecular pathways of oral cancer that predict prognosis and survival: A systematic review.

J Carcinog 2018 31;17. Epub 2018 Dec 31.

Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.

Several genes and pathways associated with oral squamous cell carcinoma (OSCC) are significant in terms of early detection and prognosis. The objective of this literature review is to evaluate the current research on molecular pathways and genes involved in oral cancer. Articles on the genes involved in oral cancer pathways were evaluated to identify potential biomarkers that can predict survival. Read More

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http://dx.doi.org/10.4103/jcar.JCar_17_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334533PMC
December 2018

Evaluation of antimitotic and antiangiogenic effect of preoperative subconjunctival application of mitomycin C in primary pterygium: a randomized trial.

Int Ophthalmol 2019 Feb 13. Epub 2019 Feb 13.

University of São Paulo (USP), São Paulo, Brazil.

Purpose: To evaluate the influence of preoperative mitomycin C (MMC) on the proliferative behavior of fibroblasts and fibrovascular tissue derived from the primary pterygium using the immunohistochemical method (Ki67 and CD34).

Design: Randomized clinical trial.

Subjects, Participants And/or Controls: Sixty-five patients with primary pterygium were randomly selected and divided into one of three groups. Read More

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http://dx.doi.org/10.1007/s10792-019-01081-0DOI Listing
February 2019

The molecular mechanisms underlying reduced E-cadherin expression in invasive ductal carcinoma of the breast: high throughput analysis of large cohorts.

Mod Pathol 2019 Feb 13. Epub 2019 Feb 13.

Nottingham Breast Cancer Research Centre, Division of Cancer and Stem Cells, School of Medicine, University of Nottingham, Nottingham, UK.

E-cadherin is a tumor suppressor gene in invasive lobular breast cancer. However, a proportion of high-grade ductal carcinoma shows reduced/loss of E-cadherin. In this study, we assessed the underlying mechanisms and molecular implications of E-cadherin loss in invasive ductal carcinoma. Read More

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http://dx.doi.org/10.1038/s41379-019-0209-9DOI Listing
February 2019

The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer.

J Immunother Cancer 2019 Feb 13;7(1):45. Epub 2019 Feb 13.

Tumor Immunology Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy.

Background: The thymic stromal lymphopoietin (TSLP), a key cytokine for development of Th2 immunity, is produced by cancer associated fibroblasts (CAFs) in pancreatic cancer where predominant tumor infiltrating Th2 over Th1 cells correlates with reduced patients' survival. Which cells and molecules are mostly relevant in driving TSLP secretion by CAFs in pancreatic cancer is not defined.

Methods: We performed in vitro, in vivo and ex-vivo analyses. Read More

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http://dx.doi.org/10.1186/s40425-019-0521-4DOI Listing
February 2019
1 Read

Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC).

EBioMedicine 2019 Feb 7. Epub 2019 Feb 7.

Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA; Clement J. Zablocki VA Medical Center, 5000 West National Avenue, Milwaukee, WI, 53295, USA. Electronic address:

Background: Autosomal Recessive Polycystic Kidney Disease (ARPKD) is marked by cyst formation in the renal tubules, primarily in the collecting duct (CD) system, ultimately leading to end-stage renal disease. Patients with PKD are generally advised to restrict their dietary sodium intake. This study was aimed at testing the outcomes of dietary salt manipulation in ARPKD. Read More

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http://dx.doi.org/10.1016/j.ebiom.2019.01.006DOI Listing
February 2019
1 Read

Ligustrazin increases lung cell autophagy and ameliorates paraquat-induced pulmonary fibrosis by inhibiting PI3K/Akt/mTOR and hedgehog signalling via increasing miR-193a expression.

BMC Pulm Med 2019 Feb 11;19(1):35. Epub 2019 Feb 11.

Department of Emergency, the First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunan, People's Republic of China.

Background: Reactive oxygen species (ROS) levels largely determine pulmonary fibrosis. Antioxidants have been found to ameliorate lung fibrosis after long-term paraquat (PQ) exposure. The effects of antioxidants, however, on the signalling pathways involved in PQ-induced lung fibrosis have not yet been investigated sufficiently. Read More

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http://dx.doi.org/10.1186/s12890-019-0799-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371511PMC
February 2019

HSP90 and Its Novel Co-Chaperones, SGT1 and CHP-1, in Brain of Patients with Parkinson's Disease and Dementia with Lewy Bodies.

J Parkinsons Dis 2019 ;9(1):97-107

Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.

Background: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the presence of inclusions known as Lewy bodies in some brain regions. Lewy bodies consist of α-synuclein and many other proteins including chaperones.

Objective: To learn more about the role of chaperone complexes in PD and a related disorder, i. Read More

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http://dx.doi.org/10.3233/JPD-181443DOI Listing
January 2019
1 Read

Clinical significance of cancer-associated fibroblasts and their correlation with microvessel and lymphatic vessel density in lung adenocarcinoma.

J Clin Lab Anal 2019 Feb 8:e22832. Epub 2019 Feb 8.

Department of Oncology, Qingdao Municipal Hospital, Qingdao, China.

Background: To determine whether cancer-associated fibroblasts (CAFs) are associated with microvessel density (MVD) and lymphatic vessel density (LVD) in lung adenocarcinoma (ADC) or are not prognostic.

Methods: Ninety-three lung adenocarcinoma patients without adjuvant therapy between January 2010 and June 2011 were enrolled. CAFs, MVD, and LVD were identified by α-smooth muscle actin (α-SMA), CD34 and D2-40 staining via immunohistochemistry. Read More

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http://dx.doi.org/10.1002/jcla.22832DOI Listing
February 2019
2 Reads

Visualizing the Balbiani Body in Zebrafish Oocytes.

Methods Mol Biol 2019 ;1920:277-293

Department of Cell Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes. Read More

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http://dx.doi.org/10.1007/978-1-4939-9009-2_16DOI Listing
January 2019

Differences in neurotropism and neurotoxicity among retrograde viral tracers.

Mol Neurodegener 2019 02 8;14(1). Epub 2019 Feb 8.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Neurotropic virus-based tracers have been extensively applied in mapping and manipulation of neural circuits. However, their neurotropic and neurotoxic properties remain to be fully characterized.

Methods: Through neural circuit tracing, we systematically compared the neurotropism discrepancy among different multi-trans-synaptic and mono-synaptic retrograde viral tracers including pseudorabies virus (PRV), rabies virus (RV), and the newly engineered retro adeno-associated virus (rAAV2-retro) tracers. Read More

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http://dx.doi.org/10.1186/s13024-019-0308-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368820PMC
February 2019
1 Read

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens.

J Vis Exp 2019 Jan 21(143). Epub 2019 Jan 21.

Laboratory of Pathology, MedImmune;

Continued developments in immuno-oncology require an increased understanding of the mechanisms of cancer immunology. The immunoprofiling analysis of tissue samples from formalin-fixed, paraffin-embedded (FFPE) biopsies has become a key tool for understanding the complexity of tumor immunology and discovering novel predictive biomarkers for cancer immunotherapy. Immunoprofiling analysis of tissues requires the evaluation of combined markers, including inflammatory cell subpopulations and immune checkpoints, in the tumor microenvironment. Read More

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http://dx.doi.org/10.3791/58390DOI Listing
January 2019
4 Reads

Increased expression of CD40/TRAF1 and activation of nuclear factor-κκB-dependent proinflammatory gene expression in collagen-induced arthritis.

Scand J Rheumatol 2018 Nov;47(6):455-460

a Department of Rheumatology , The First Affiliated Hospital of Soochow University , Su Zhou , China.

Objective: Genetic studies have implicated both CD40 and tumour necrosis factor receptor-associated factor-1 (TRAF1) with rheumatoid arthritis (RA). CD40 signalling is known to be associated with TRAF1 expression, directly or indirectly; however, the detailed mechanisms of these interactions are not clear in RA, and in particular in fibroblast-like synoviocytes. This study aims to investigate this pathway and the role of nuclear factor-κB (NF-κB) in a mouse model of RA. Read More

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http://dx.doi.org/10.1080/03009742.2018.1432684DOI Listing
November 2018

Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats.

J Mol Neurosci 2019 Feb 8. Epub 2019 Feb 8.

School of Pharmacy, Ministry of Education, Shihezi University/Key Laboratory of Xingjiang Phytomedicine Resources Utilization, Shihezi, China.

1,5-O-dicaffeoyl-3-O-(4-malic acid methylester)-quinic acid (MQA), extracted from Arctium lappa L., has been observed to exert neuroprotective effects in vitro. The aim of this study was to investigate whether MQA is an effective therapeutic method for cerebral ischemic injury in vivo. Read More

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http://dx.doi.org/10.1007/s12031-019-01268-1DOI Listing
February 2019

Soy Isoflavones Ameliorate Metabolic and Immunological Alterations of Ovariectomy in Female Wistar Rats: Antioxidant and Estrogen Sparing Potential.

Oxid Med Cell Longev 2019 10;2019:5713606. Epub 2019 Jan 10.

College of Applied Medical Sciences, Department of Medical Laboratory Sciences, University of Bisha, Saudi Arabia.

Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy isoflavones as HRT on immunological and bone health-related parameters with a special focus on the interactions between immunological status and metabolism. Thirty healthy cyclic female Wistar rats were used in this experiment. Read More

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http://dx.doi.org/10.1155/2019/5713606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348823PMC
January 2019
2 Reads

A Nonsense Mitochondrial DNA Mutation Associates with Dysfunction of HIF1 in a Von Hippel-Lindau Renal Oncocytoma.

Oxid Med Cell Longev 2019 9;2019:8069583. Epub 2019 Jan 9.

Department of Medical and Surgical Sciences (DIMEC), Unit of Medical Genetics, University of Bologna Medical School, Bologna 40138, Italy.

The Von Hippel-Lindau (VHL) syndrome has been rarely associated with renal oncocytomas, and tumors usually show HIF1 chronic stabilization. By contrast, oncocytomas mainly associated with respiratory chain (RC) defects due to severe mitochondrial DNA (mtDNA) mutations are incapable of stabilizing HIF1, since oxygen consumption by the RC is dramatically diminished and prolylhydroxylase activity is increased by -ketoglutarate accumulation following Krebs cycle slowdown. Here, we investigate the cooccurrence of a pseudohypoxic condition with oncocytic transformation in a case of VHL-associated renal oncocytoma. Read More

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http://dx.doi.org/10.1155/2019/8069583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343171PMC
January 2019
3 Reads

TES functions as a Mena-dependent tumor suppressor in gastric cancer carcinogenesis and metastasis.

Cancer Commun (Lond) 2019 Feb 6;39(1). Epub 2019 Feb 6.

Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine; Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangzhou, 510060, Guangdong, P.R. China.

Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regions for the cell adhesion and cytoskeleton regulatory proteins. Mena is a known cytoskeleton regulator that regulates the assembly of actin filaments and modulates cell adhesion and motility by interacting with Lamellipodin (Lpd). Read More

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http://dx.doi.org/10.1186/s40880-019-0347-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366075PMC
February 2019

Gold Nanorods-Based Smart Nanoplatforms for Synergic Thermotherapy and Chemotherapy of Tumor Metastasis.

ACS Appl Mater Interfaces 2019 Feb 15. Epub 2019 Feb 15.

Department of Radiology, Nanjing Drum Tower Hospital , The Affiliated Hospital of Nanjing University Medical School , No. 321 Zhongshan Road , Nanjing 210008 , China.

The combination therapy of photothermal therapy and chemotherapy as a promising strategy has drawn extensive attention by overcoming the limitations of conventional treatments in tumor therapy. Gold nanorods-based nanoplatforms were herein designed by integrating doxorubicin (DOX)- and polydopamine-coated gold nanorods (GNRs@PDA) for tumor metastasis inhibition and multifunctional drug delivery. The GNRs@PDA-poly(ethylene glycol) (PEG)-DOX nanocomplex showed robust stability and excellent near-infrared (NIR) photothermal conversion efficiency under laser irradiation. Read More

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http://dx.doi.org/10.1021/acsami.8b21784DOI Listing
February 2019
2 Reads

Establishment and evaluation of a simulated high‑altitude hypoxic brain injury model in SD rats.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Wenjiang, Chengdu, Sichuan 611137, P.R. China.

This study was conducted to establish a stable hypobaric hypoxia brain injury model. SD rats were randomly separated into control and model groups, and placed outside or inside of a hypobaric chamber, respectively. Subsequent to 24 h anoxic exposure, plasma superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG) and lactate dehydrogenase (LDH) were measured using commercial biochemical kits. Read More

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http://dx.doi.org/10.3892/mmr.2019.9939DOI Listing
February 2019
2 Reads
1.484 Impact Factor

The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer.

Immunopharmacol Immunotoxicol 2019 Feb 1:1-7. Epub 2019 Feb 1.

h National Laboratory Animal Center , National Applied Research Laboratories , Taipei , Taiwan.

Objective: Inflammatory bowel disease (IBD) is generally considered as a major risk factor in the progression of colitis-associated colorectal cancer (CAC). Previous studies have indicated that the composition of gut microflora may be involved in CAC induction and progress. Bacteroides fragilis (BF) is a Gram-negative anaerobe belonging to colonic symbiotic bacteria of the host. Read More

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http://dx.doi.org/10.1080/08923973.2019.1569047DOI Listing
February 2019
1 Read

Metformin as an add-on to insulin improves periodontal response during orthodontic tooth movement in type 1 diabetic rats.

J Periodontol 2019 Jan 30. Epub 2019 Jan 30.

Department of Biological Sciences, School of Dentistry of Bauru, São Paulo University, Bauru, São Paulo, Brazil.

Background: Type 1 diabetes (T1D) is associated with delayed tissue healing and bone loss. Periodontal tissues during tooth movement (OTM) in T1D and under diabetic treatment are poorly understood. We aimed to study the effect of metformin as an add-on to insulin therapy on periodontal structures during OTM in T1D rats. Read More

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http://dx.doi.org/10.1002/JPER.18-0140DOI Listing
January 2019

Epithelium segmentation using deep learning in H&E-stained prostate specimens with immunohistochemistry as reference standard.

Sci Rep 2019 Jan 29;9(1):864. Epub 2019 Jan 29.

Radboud University Medical Center, Diagnostic Image Analysis Group and the Department of Pathology, 6500HB, Nijmegen, The Netherlands.

Given the importance of gland morphology in grading prostate cancer (PCa), automatically differentiating between epithelium and other tissues is an important prerequisite for the development of automated methods for detecting PCa. We propose a new deep learning method to segment epithelial tissue in digitised hematoxylin and eosin (H&E) stained prostatectomy slides using immunohistochemistry (IHC) as reference standard. We used IHC to create a precise and objective ground truth compared to manual outlining on H&E slides, especially in areas with high-grade PCa. Read More

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http://dx.doi.org/10.1038/s41598-018-37257-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351532PMC
January 2019

Reversible SAHH inhibitor protects against glomerulonephritis in lupus-prone mice by downregulating renal α-actinin-4 expression and stabilizing integrin-cytoskeleton linkage.

Arthritis Res Ther 2019 Jan 29;21(1):40. Epub 2019 Jan 29.

Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Background: Glomerulonephritis is one of the major complications and causes of death in systemic lupus erythematosus (SLE) and is characterized by glomerulosclerosis, interstitial fibrosis, and tubular atrophy, along with severe persistent proteinuria. DZ2002 is a reversible S-adenosyl-L-homocysteine hydrolase (SAHH) inhibitor with potent therapeutic activity against lupus nephritis in mice. However, the molecular events underlying the renal protective effects of DZ2002 remained unclear. Read More

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http://dx.doi.org/10.1186/s13075-019-1820-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352376PMC
January 2019
2 Reads

Connective tissue growth factor mediates transforming growth factor β-induced collagen expression in human endometrial stromal cells.

PLoS One 2019 29;14(1):e0210765. Epub 2019 Jan 29.

School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan.

Background: Adenomyosis is a medical condition defined by the abnormal presence of endometrial tissue within the myometrium, in which fibrosis occurs with new collagen deposition and myofibroblast differentiation. In this study, the effect of several mediators and growth factors on collagen expression was investigated on human endometrial stromal cells (fibroblasts) derived from adenomyotic endometrium.

Experimental Approach: RT-PCR, Western blot analysis, pharmacological interventions and siRNA interference were applied to primary cultured human endometrial stromal cells (fibroblasts). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210765PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350958PMC
January 2019

Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology.

J Histochem Cytochem 2019 Jan 29:22155419826940. Epub 2019 Jan 29.

Otopathology Laboratory, Massachusetts Eye and Ear Infirmary, Massachusetts, University Hospital Zürich, Zürich, Switzerland.

Heat-induced antigen retrieval (HIAR) is routinely employed on aldehyde-fixed tissue sections to enhance the reactivity of antibodies that exhibit weak or no specific interactions with tissue antigens when applied in conventional immunohistochemical protocols. A major drawback of HIAR protocols is, however, the heat-induced detachment of sections from the microscope slide with resultant impaired tissue morphology or loss of the section. We developed a method in which tissue sections mounted on glass slides are temporally coverslipped, and a clamp is used to compress the sections on the microscope slide during HIAR treatment. Read More

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http://journals.sagepub.com/doi/10.1369/0022155419826940
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http://dx.doi.org/10.1369/0022155419826940DOI Listing
January 2019
3 Reads

Overexpression of oncostatin M receptor regulates local immune response in glioblastoma.

J Cell Physiol 2019 Jan 28. Epub 2019 Jan 28.

Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, Liaoning, China.

Glioblastoma (GBM) is the most lethal cancer in central nervous system. It is urgently needed to look for novel therapeutics for GBM. Oncostatin M receptor (OSMR) is a cytokine receptor gene of IL-6 family and has been reported to be involved in regulating GBM tumorigenesis. Read More

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http://doi.wiley.com/10.1002/jcp.28197
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http://dx.doi.org/10.1002/jcp.28197DOI Listing
January 2019
5 Reads

ZNF326 promotes malignant phenotype of glioma by up-regulating HDAC7 expression and activating Wnt pathway.

J Exp Clin Cancer Res 2019 Jan 28;38(1):40. Epub 2019 Jan 28.

Department of Pathology, College of Basic Medical Sciences, and First Affiliated Hospital of China Medical University, Shenyang, China.

Background: Zinc-finger protein-326 (ZNF326) was initially found in the NIH3T3 cell line to regulate cell growth, however, the expression and underlying role of ZNF326 in human tumours, especially in glioma, is not fully understood.

Methods: Immunohistochemistry was applied to detect the expression of ZNF326 in glioma tissues, and statistical analysis was used to analyse the relationship between ZNF326 expression and clinicopathological factors. The effect of ZNF326 on glioma cells proliferation and invasion was conducted by functional experiments both in vivo and in vitro. Read More

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http://dx.doi.org/10.1186/s13046-019-1031-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350303PMC
January 2019

Esculetin enhances the inhibitory effect of 5-Fluorouracil on the proliferation, migration and epithelial-mesenchymal transition of colorectal cancer.

Cancer Biomark 2019 Jan 18. Epub 2019 Jan 18.

Department of Oncology, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, China.

Background: Colorectal cancer (CRC) is the most common malignant disease worldwide and thus new therapeutic approaches are needed. 5-Fluorouracil (5-FU) remains the most widely used agent to treat colorectal cancer (CRC). However, its clinical efficacy is currently limited by the development of drug resistance. Read More

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http://dx.doi.org/10.3233/CBM-181764DOI Listing
January 2019
1 Read

Postnatal calpeptin treatment causes hippocampal neurodevelopmental defects in neonatal rats.

Neural Regen Res 2019 May;14(5):834-840

Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, Anhui Province, China.

Our previous studies showed that the early use of calpain inhibitors reduces calpain activity in multiple brain regions, and that postnatal treatment with calpeptin may lead to cerebellar motor dysfunction. However, it remains unclear whether postnatal calpeptin application affects hippocampus-related behaviors. In this study, Sprague-Dawley rats were purchased from the Animal Center of Anhui Medical University of China. Read More

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http://www.nrronline.org/text.asp?2019/14/5/834/249231
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http://dx.doi.org/10.4103/1673-5374.249231DOI Listing
May 2019
3 Reads

Expression and Roles of S100A4 in Anaplastic Cells of Canine Mammary Carcinomas.

Vet Pathol 2019 Jan 27:300985818823772. Epub 2019 Jan 27.

2 Department of Veterinary Pathology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.

S100A4 (metastasin), a member of the S100 protein family, was initially identified in metastatic cells and is well established as a marker of aggressive human cancer. However, expression and roles of S100A4 in canine mammary tumors have not been clarified. In this study, expression of S100A4 was examined immunohistochemically in normal, hyperplastic, and neoplastic mammary glands of dogs. Read More

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http://dx.doi.org/10.1177/0300985818823772DOI Listing
January 2019
2 Reads

Immunohistochemical Grading of Epidural Fibrosis with CD105 Antibody.

World Neurosurg 2019 Jan 24. Epub 2019 Jan 24.

University of Health Sciences, Bakırköy Prof. Dr. Mazhar Osman Training and Research Hospital for Neurology, Neurosurgery and Psychiatry, İstanbul, Turkey.

Purpose: Grading of epidural fibrosis is usually performed by histopathological staining in experimental studies. Immunohistochemical methods for grading are not available in routine practice yet. In our study, the effect of tranexamic acid (TXA), a commonly used hemostatic agent in surgical interventions, was evaluated for use against the development of EF with classical histopathological methods and immunohistochemistry using the CD105 antibody, a marker of angiogenesis. Read More

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http://dx.doi.org/10.1016/j.wneu.2019.01.068DOI Listing
January 2019
2 Reads

Metastatic Immune Infiltrates Correlate with Those of the Primary Tumor in Canine Osteosarcoma.

Vet Comp Oncol 2019 Jan 25. Epub 2019 Jan 25.

The Comparative Oncology Laboratory and Center for Companion Animal Health, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.

Our lack of understanding of the immune microenvironment in canine osteosarcoma (cOSA) has limited the identification of potential immunotherapeutic targets. In particular, our ability to utilize readily available tissue from a dog's primary tumor to predict the type and extent of immune response in their pulmonary metastatic lesions is unknown. We therefore collected 21 matched pairs of primary tumors and pulmonary metastatic lesions from dogs with OSA and performed immunohistochemistry to quantify T lymphocyte (CD3), FOXP3+ cell, B lymphocyte (Pax-5), and CD204+ macrophage infiltration. Read More

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http://dx.doi.org/10.1111/vco.12459DOI Listing
January 2019
5 Reads

PMab-210: A Monoclonal Antibody Against Pig Podoplanin.

Monoclon Antib Immunodiagn Immunother 2019 Jan 25. Epub 2019 Jan 25.

1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.

Podoplanin (PDPN) is a type I transmembrane glycoprotein that is expressed in normal tissues, including renal corpuscles and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs have not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and screened hybridomas, which are producing anti-pPDPN mAbs. Read More

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http://dx.doi.org/10.1089/mab.2018.0038DOI Listing
January 2019

Dissecting the predictive value of MAPK/AKT/estrogen-receptor phosphorylation axis in primary breast cancer to treatment response for tamoxifen over exemestane: a Translational Report of the Intergroup Exemestane Study (IES)-PathIES.

Breast Cancer Res Treat 2019 Jan 24. Epub 2019 Jan 24.

Department of Cancer and Surgery, Faculty of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.

Purpose: The prognostic and predictive values of the MAPK/AKT/ERα phosphorylation axis (pT202/T204MAPK, pT308AKT, pS473AKT, pS118ERα and pS167ERα) in primary tumours were assessed to determine whether these markers can differentiate between patient responses for switching adjuvant endocrine therapy after 2-3 years from tamoxifen to exemestane and continued tamoxifen monotherapy in the Intergroup Exemestane Study (IES).

Methods: Of the 4724 patients in IES, 1506 were managed in a subset of centres (N = 89) participating in PathIES. These centres recruited 1282 (85%, 1282/1506) women into PathIES of whom 1036 had phospho-marker data. Read More

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http://dx.doi.org/10.1007/s10549-018-05110-xDOI Listing
January 2019

JMJD3 expression is an independent prognosticator in patients with esophageal squamous cell carcinoma.

Surgery 2019 Jan 21. Epub 2019 Jan 21.

Department of Applied Chemistry, and Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou, Taiwan; Guangdong Institute of Gastroenterology, and Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, Sun Yat-sen University, Guangzhou, China. Electronic address:

Background: The Jumonji-domain containing 3 has diverse roles in multiple cancers. Here, we investigated its prognostic significance in esophageal squamous cell carcinoma.

Methods: By using immunohistochemistry, the Jumonji-domain containing 3 expression was examined in 109 surgically resected esophageal squamous cell carcinomas and correlated with treatment outcome. Read More

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http://dx.doi.org/10.1016/j.surg.2018.11.015DOI Listing
January 2019
1 Read

Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/β-catenin signaling.

J Exp Clin Cancer Res 2019 Jan 24;38(1):33. Epub 2019 Jan 24.

State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.

Background: The primary obstacle to treat cervical cancer is its high prevalence of metastasis, which severely affects patients' quality of life and survival time. Nucleolar and spindle associated protein 1 (NUSAP1) has been implicated in the development, progression, and metastasis in several types of cancer. However, its oncogenic role in cervical cancer remains unclear. Read More

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http://dx.doi.org/10.1186/s13046-019-1037-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346521PMC
January 2019
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Looking for A Place for Dose-Dense TMZ Regimens in GBM Patients: An Experience with MGMT Exploratory Evaluation.

Bioengineering (Basel) 2019 Jan 22;6(1). Epub 2019 Jan 22.

Medical Oncology, St Salvatore Hospital, 67100 L'Aquila, Italy.

Prolonged exposure to temozolomide (TMZ) could improve clinical outcomes in recurrent glioblastoma multiforme (GBM) patients. We previously developed a dose-dense regimen of TMZ in a phase II study (180 mg/m2 from days 1 to 5 every two weeks). A retrospective analysis of patients with macroscopic residual GBM treated with "post-induction" dose-dense TMZ was conducted, adding an explorative subgroup analyses among patients with different O6-methylguanine DNA methyltransferase (MGMT) expressions (negative vs positive, < vs ≥ of 50 % of cells stained, < vs ≥ 70% of cells stained). Read More

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http://dx.doi.org/10.3390/bioengineering6010011DOI Listing
January 2019

Analyses on K-ras mutations and fascin expression in patients with cardia cancer.

Oncol Lett 2019 Feb 22;17(2):1807-1811. Epub 2018 Nov 22.

Department of Pathology, Jining Tumor Hospital, Jining, Shandong 272000, P.R. China.

Mutations of K-rat sarcoma (K-ras) in patients with cardia cancer and their effects on the expression of fascin were investigated. A total of 90 cardia cancer patients treated in Jining First People's Hospital from March 2014 to March 2017 were randomly selected. Genomic deoxyribonucleic acid (DNA) was extracted from paraffin-embedded cardia cancer specimens. Read More

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http://www.spandidos-publications.com/10.3892/ol.2018.9750
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http://dx.doi.org/10.3892/ol.2018.9750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341761PMC
February 2019
6 Reads
0.987 Impact Factor

Imaging of Metastatic Cancer Cells in Sentinel Lymph Nodes using Affibody Probes and Possibility of a Theranostic Approach.

Int J Mol Sci 2019 Jan 19;20(2). Epub 2019 Jan 19.

Division of Functional Imaging, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba 277-8577, Japan.

The accurate detection of lymph node metastases is essential for treatment success in early-stage malignant cancer. Sentinel lymph node (SLN) biopsy is the most effective procedure for detecting small or micrometastases that are undetectable by conventional imaging modalities. To demonstrate a new approach for developing a more efficient SLN biopsy procedure, we reported a two-stage imaging method combining lymphoscintigraphy and near-infrared (NIR) fluorescence imaging to depict metastatic cancer cells in SLNs in vivo. Read More

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http://dx.doi.org/10.3390/ijms20020427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359136PMC
January 2019
1 Read

Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund's complete adjuvant-induced arthritis in rats.

Phytomedicine 2019 Feb 25;53:223-233. Epub 2018 Jul 25.

Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China. Electronic address:

Background: Discovering novel compounds with higher activities is a key aim of natural products research. Gardenia jasminoides Ellis is a herb with anti-inflammatory properties. Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in this herb and are considered its active ingredients. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.07.005DOI Listing
February 2019
5 Reads

Image Analysis of HER2 Immunohistochemical Staining of Surgical Breast Cancer Specimens.

Yonsei Med J 2019 Feb;60(2):158-162

Department of Hospital Pathology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Purpose: Trastuzumab is an effective treatment for human epidermal growth factor receptor 2 (HER2)-amplified breast cancers. We sought to develop a simple protocol for HER2 image analysis of breast cancer specimens.

Materials And Methods: In a preliminary test, we found that at least 1000 tumor cells need to be examined in the most strongly stained areas. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3349/ymj.2019.60
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http://dx.doi.org/10.3349/ymj.2019.60.2.158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342717PMC
February 2019
7 Reads

Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis.

Lab Invest 2019 Jan 21. Epub 2019 Jan 21.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000, China.

Homeobox B7 (HOXB7) protein is reported to be aberrantly expressed in a variety of cancers and to play an important role in multiple cellular processes. However, the specific mechanism by which HOXB7 promotes the malignant progression of intrahepatic cholangiocarcinoma (ICC) remains unclear. Therefore, we used quantitative real-time polymerase chain reaction (PCR) to detect the expression level of HOXB7 in 38 paired ICC tissue samples. Read More

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http://dx.doi.org/10.1038/s41374-018-0150-4DOI Listing
January 2019
5 Reads
3.676 Impact Factor

Circular RNA circ_0001946 acts as a competing endogenous RNA to inhibit glioblastoma progression by modulating miR-671-5p and CDR1.

J Cell Physiol 2019 Jan 21. Epub 2019 Jan 21.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

Objectives: In many malignant tumors, circRNAs play an important role. However, the biological role and clinical significance of circRNAs remain unclear. In this study, we investigated the effects of circ_0001946 on the progression of glioblastoma (GBM) and the molecular mechanism of circ_0001946. Read More

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http://dx.doi.org/10.1002/jcp.28061DOI Listing
January 2019
9 Reads

Extracellular matrix deposited by Wharton's jelly mesenchymal stem cells enhances cell expansion and tissue specific lineage potential.

Am J Transl Res 2018 15;10(11):3465-3480. Epub 2018 Nov 15.

Department of Orthopaedics, Zhongshan Hospital, Fudan University Shanghai, China.

This article aims to explore whether Wharton's jelly (WJ) derived mesenchymal stem cells (MSCs) (WJ-MSCs) decellularized extracellular matrix (dECM) can rejuvenate MSCs during expansion. Passage 10 synovium-derived mesenchymal stem cells (SDSCs) and WJ-MSCs were expanded on plastic flasks (PL) or dECMs derived from SDSCs and WJ-MSCs. Flow cytometry was applied to evaluate surface phenotypes and proliferation capacity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291692PMC
November 2018
1 Read

Unlocking bone for proteomic analysis and FISH.

Lab Invest 2019 Jan 18. Epub 2019 Jan 18.

Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.

Bone tissue is critically lagging behind soft tissues and biofluids in our effort to advance precision medicine. The main challenges have been accessibility and the requirement for deleterious decalcification processes that impact the fidelity of diagnostic histomorphology and hinder downstream analyses such as fluorescence in-situ hybridization (FISH). We have developed an alternative fixation chemistry that simultaneously fixes and decalcifies bone tissue. Read More

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http://www.nature.com/articles/s41374-018-0168-7
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http://dx.doi.org/10.1038/s41374-018-0168-7DOI Listing
January 2019
4 Reads