21,321 results match your criteria Applied Immunohistochemistry & Molecular Morphology[Journal]


The zinc finger protein GATA4 induces MET and cellular senescence through the NF-κB pathway in hepatocellular carcinoma.

J Gastroenterol Hepatol 2019 Apr 17. Epub 2019 Apr 17.

Laboratory Animal Center, Chongqing Medical University, Chongqing.

Background And Aim: The high mortality and poor prognosis of hepatocellular carcinoma (HCC) have raised the public attention. Gene therapy is considered as a promising treatment option for cancer; thus, finding a new therapeutic target for hepatocellular carcinoma is urgently needed. GATA4 is a tumor suppressor gene in multiple cancers, but its role in HCC is unclear. Read More

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http://dx.doi.org/10.1111/jgh.14684DOI Listing

A Machine Learning Platform to Optimize the Translation of Personalized Network Models to the Clinic.

JCO Clin Cancer Inform 2019 Apr(3):1-17

1 Royal College of Surgeons in Ireland, Dublin, Ireland.

Purpose: Dynamic network models predict clinical prognosis and inform therapeutic intervention by elucidating disease-driven aberrations at the systems level. However, the personalization of model predictions requires the profiling of multiple model inputs, which hampers clinical translation.

Patients And Methods: We applied APOPTO-CELL, a prognostic model of apoptosis signaling, to showcase the establishment of computational platforms that require a reduced set of inputs. Read More

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http://dx.doi.org/10.1200/CCI.18.00056DOI Listing

Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology.

J Cytol 2019 Apr-Jun;36(2):79-83

Department of Pathology, Burdwan Medical College, Burdwan, India.

Background: The cytological examination of serous body effusions to diagnose and stage malignancy is well accepted in clinical medicine. Conventional smear (CS) and cell block (CB) study has to be complemented with immunohistochemistry (IHC) for a definitive diagnosis of malignancy and also to differentiate it from reactive mesothelial cells. Cytology microarray (CMA) is a modification of tissue microarray which involves core needle biopsy of multiple cell blocks and embedding it in a single block. Read More

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http://dx.doi.org/10.4103/JOC.JOC_15_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425782PMC

Differential expressions of PD-1, PD-L1 and PD-L2 between primary and metastatic sites in renal cell carcinoma.

BMC Cancer 2019 Apr 16;19(1):360. Epub 2019 Apr 16.

Department of Urology, West China Hospital, Sichuan University, Chengdu, People's Republic of China, 610041.

Background: In clinical practice, the detection of biomarkers is mostly based on primary tumors for its convenience in acquisition. However, immune checkpoints may express differently between primary and metastatic tumor. Therefore, we aimed to compare the differential expressions of PD-1, PD-L1 and PD-L2 between the primary and metastatic sites of renal cell carcinoma (RCC). Read More

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http://dx.doi.org/10.1186/s12885-019-5578-4DOI Listing

The Ameliorating Effect of Plasma Protein from on Cyclophosphamide-Induced Acute Kidney Injury in Mice.

Mar Drugs 2019 Apr 15;17(4). Epub 2019 Apr 15.

Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.

In the study, the protective effect of plasma protein from (PPTT) on acute kidney injury (AKI) and the related molecular mechanisms were first investigated by Western blotting analyses, TdT-mediated dUTP Nick-End Labeling (TUNEL) assay, and immunohistochemistry. It was found that PPTT had an obviously inhibitory effect on Reactive oxygen species (ROS) in cyclophosphamide (CTX)-exposed mice. Furthermore, results demonstrated that the renal cell death mode is due to inducing apoptosis and autophagy inhibited by dose-dependent PPTT in mice treated with CTX by decreasing the protein expression of bax, beclin-1, and LC3 and increasing the expression of bcl-2. Read More

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http://dx.doi.org/10.3390/md17040227DOI Listing

Low-level laser as a complementary therapy in orofacial granulomatosis management: a case report.

Oral Surg Oral Med Oral Pathol Oral Radiol 2019 Mar 1. Epub 2019 Mar 1.

Department of Stomatology and Oral and Maxillofacial Surgery, Hospital Metropolitano Odilon Behrens (HMOB), Belo Horizonte, MG, Brazil.

Orofacial granulomatosis (OFG) is a chronic inflammatory disease that typically affects the soft tissues of the orofacial region. The most common clinical manifestation of OFG is lip swelling, which may be associated with intrabuccal ulcerations and increased growth of the gingiva and mucosa, as well as cutaneous erythema and facial edema. The treatment for OFG is challenging, and sometimes considered unsatisfactory. Read More

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http://dx.doi.org/10.1016/j.oooo.2019.02.024DOI Listing

Immune checkpoint molecule herpes virus entry mediator is overexpressed and associated with poor prognosis in human glioblastoma.

EBioMedicine 2019 Apr 12. Epub 2019 Apr 12.

Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Key Laboratory of Brain Functional Remodeling, Shandong, 107# Wenhua Xi Road, Jinan 250012, China. Electronic address:

Background: Dysregulation of immune checkpoint molecules leads to immune evasion in human tumours but has become a viable target for tumour therapy. Here, we examined expression of Herpes virus entry mediator (HVEM), an immune checkpoint molecule, in human glioblastoma (GBM) to assess its potential as a molecular target for treatment.

Methods: Molecular and clinical data from publicly available genomic databases containing WHO grade II-IV human glioma cases (n = 1866) were analyzed. Read More

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http://dx.doi.org/10.1016/j.ebiom.2019.04.002DOI Listing

Spontaneous Cholangiofibrosis in a Wistar Rat.

Toxicol Pathol 2019 Apr 15:192623319842549. Epub 2019 Apr 15.

2 Experimental Pathology Laboratories, Inc., Seattle, Washington, USA.

In a 2-year carcinogenicity study, we identified a spontaneous cholangiofibrosis in a control male Wistar rat. This lesion has long been considered as a compound-related change, with no spontaneous cases reported in the Wistar rat. In addition to routine hematoxylin and eosin stains evaluation, we applied Masson's trichrome staining, Alcian blue-periodic acid-Schiff staining, and OV-6 immunohistochemistry staining. Read More

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http://dx.doi.org/10.1177/0192623319842549DOI Listing

Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects.

Int J Mol Sci 2019 Apr 3;20(7). Epub 2019 Apr 3.

Department of Cell Biology and Histology, School of Medicine and Nursing, University of the Basque Country, BioCruces Health Research Institute, Begiker, 48940 Leioa, Spain.

The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. Read More

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https://www.mdpi.com/1422-0067/20/7/1655
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http://dx.doi.org/10.3390/ijms20071655DOI Listing
April 2019
1 Read

Endoplasmic reticulum protein 29 (ERp29) as a novel prognostic marker and tumor suppressor in osteosarcoma.

J Bone Oncol 2019 Jun 19;16:100233. Epub 2019 Mar 19.

Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Background: Diverse aberrancy in genetic background, protein profiling, and biological pathways have emerged as important factors hindering discovery of effective treatment of osteosarcoma. In a previous study, we used a proteomic approach to identify some osteosarcoma-related proteins by analysis of protein profiling in individual patients through primary cell culture. Endoplasmic reticulum protein 29 (ERp29) emerged as a protein of interest for further study since accumulating evidence suggests it has broad functions in tumorigenesis of different types of cancer. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22121374183031
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http://dx.doi.org/10.1016/j.jbo.2019.100233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444297PMC
June 2019
2 Reads

The effects of dihydroxyphenyl lactic acid on alleviating blood-brain barrier injury following subarachnoid hemorrhage in rats.

Neurosci Lett 2019 Apr 10. Epub 2019 Apr 10.

Department of Anatomazy and Histology, School of Basic Medical Sciences, Peking University, Beijing, 100191, China; Beijing Key Lab of Magnetic Resonance Imaging Technology, Beijing, 100191, China. Electronic address:

Background: In this study, the protective effects of 3,4-dihydroxyphenyl lactic acid (DLA) on blood-brain barrier (BBB) injury following subarachnoid hemorrhage (SAH) has been explored.

Methods: Male Sprague-Dawley rats (weight 300-350 g) were used to establish the SAH model using the endovascular perforation method. The animals were randomly divided into four groups: sham (n = 40), SAH (n = 46), SAH + vehicle (n = 44), and SAH + DLA (n = 40) treatment groups. Read More

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http://dx.doi.org/10.1016/j.neulet.2019.04.025DOI Listing

CD70 expression in tumor-associated fibroblasts predicts worse survival in colorectal cancer patients.

Virchows Arch 2019 Apr 13. Epub 2019 Apr 13.

Department of Pathology, Aichi Medical University School of Medicine, Nagakute1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.

The anticancer effects of immune checkpoint inhibitors against CTLA4 and CD274-PDCD1 axes are evident. However, these immunotherapies for colorectal cancers (CRCs) are now limited to a small subset of patients with microsatellite unstable tumors. Thus, therapeutics targeting other types of CRCs is desired. Read More

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http://link.springer.com/10.1007/s00428-019-02565-1
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http://dx.doi.org/10.1007/s00428-019-02565-1DOI Listing
April 2019
5 Reads

Mulberrin attenuates 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced Parkinson's disease by promoting Wnt/β-catenin signaling pathway.

J Chem Neuroanat 2019 Apr 9;98:63-70. Epub 2019 Apr 9.

Mudanjiang Medical University, Affiliated Hongqi Hospital, Department of Neurology, Ward 2, Mudanjiang, 157000, Heilongjiang, China. Electronic address:

Abnormal neuroinflammation and oxidative stress has been shown to cause neuronal loss in the progressive neurodegenerative Parkinson's disease (PD). Mulberrin is the key component of Ramulus Mori that has various biological activities. This study was to investigate the functions and mechanisms of mulberrin in PD. Read More

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http://dx.doi.org/10.1016/j.jchemneu.2019.04.003DOI Listing

Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion.

Kaohsiung J Med Sci 2019 Apr 12. Epub 2019 Apr 12.

Department of Emergency, Minhang Hospital, Fudan University, Shanghai, China.

Limb ischemia reperfusion (LIR) can activate endogenous cytoprotective mechanisms by generating specific proteins against reperfusion injury in remote organs. The present study investigated the roles of heme oxygenase-1 (HO-1) pathway and the molecular mechanisms underlying the regulation of this pathway on lung injury following LIR. LIR was induced by ischemia for 4 hours followed by reperfusion for 6 hours (LIR 6 hours) or 16 hours (LIR 16 hours) in male Sprague-Dawley rats. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/kjm2.12070
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http://dx.doi.org/10.1002/kjm2.12070DOI Listing
April 2019
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Dietary heat-killed Lactobacillus brevis SBC8803 (SBL88™) improves hippocampus-dependent memory performance and adult hippocampal neurogenesis.

Neuropsychopharmacol Rep 2019 Apr 11. Epub 2019 Apr 11.

Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.

Aims: Lactobacillus species are used widely as various food and supplements to improve health. Previous studies have shown that heat-killed Lactobacillus brevis SBC8803 induces serotonin release from intestinal cells and affects sleep rhythm and the autonomic nervous system. However, the effect of SBC8803 on cognitive function remains unknown. Read More

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http://dx.doi.org/10.1002/npr2.12054DOI Listing

Differential severity of LPS-induced lung injury in CD26/DPP4 positive and deficient F344 rats.

Histol Histopathol 2019 Apr 12:18117. Epub 2019 Apr 12.

Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.

Background: Lipopolysaccharide (LPS) induced inflammation often leads to lung injury, in which pulmonary recruitment of neutrophils plays a pivotal role. Inflammatory processes are influenced by CD26/DPP4, highly expressed in lungs. Asthma induced CD26/DPP4 deficient (CD26/DPP4⁻) Fischer (F) 344 rats suffering from a transport block in the rER caused by a point mutation showed reduced pulmonary inflammation and reduced expression of immunomodulating surfactant proteins (SP). Read More

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http://www.hh.um.es/Abstracts/Vol_/_/__18117.htm
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http://dx.doi.org/10.14670/HH-18-117DOI Listing
April 2019
1 Read

Mucosal Human Papillomavirus Detection and TP53 Immunohistochemical expression in Non-Melanoma Skin Cancer in Tunisian Patients.

J Cutan Pathol 2019 Apr 11. Epub 2019 Apr 11.

Department of Human and Experimental Pathology, Institut Pasteur de Tunis, Tunis, Tunisia.

Background: Several studies have reported the oncogenic role of human papillomavirus (HPV) in non -melanoma skin cancer (NMSC) carcinogenesis. Considering that HPV could affect TP53 degradation via E6 oncoprotein, we evaluated the expression of TP53 according to HPV infection and E6 expression.

Methods: Biopsy specimens from 79 NMSCs (28 squamous cell carcinomas, 21 keratoacanthomas and 30 basal cell carcinomas) were enrolled. Read More

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http://dx.doi.org/10.1111/cup.13473DOI Listing
April 2019
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Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.

Front Physiol 2019 26;10:306. Epub 2019 Mar 26.

Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Toll-like receptor (TLR)3 activation during the neonatal period produces responses linked to the origins of neuropsychiatric disorders. Although there is sexual dimorphism in neuropsychiatric disorders, it is unknown if brain responses to TLR3 activation are sex-specific. We hypothesized that poly I:C in a post-natal day (P)8 model induces a sexually dimorphic inflammatory responses. Read More

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http://dx.doi.org/10.3389/fphys.2019.00306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443881PMC

Histomorphological study of prostatic adenocarcinoma and its mimics.

Indian J Pathol Microbiol 2019 Apr-Jun;62(2):251-260

Department of Pathology, Government Medical College, Bolangir, Odisha, India.

Context: Prostate adenocarcinoma (PC) is one of the common cancers in India and world over. Numerous prostatic, nonprostatic lesions, and normal structures can be very similar to adenocarcinoma. A pathologist's awareness of the benign mimics is important for the diagnosis of PC. Read More

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http://dx.doi.org/10.4103/IJPM.IJPM_322_18DOI Listing

CD73 promotes hepatocellular carcinoma progression and metastasis via activating PI3K/AKT signaling by inducing Rap1-mediated membrane localization of P110β and predicts poor prognosis.

J Hematol Oncol 2019 Apr 11;12(1):37. Epub 2019 Apr 11.

Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai, 200032, People's Republic of China.

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide because of rapid progression and high incidence of metastasis or recurrence. Accumulating evidence shows that CD73-expressing tumor cell is implicated in development of several types of cancer. However, the role of CD73 in HCC cell has not been systematically investigated and its underlying mechanism remains elusive. Read More

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http://dx.doi.org/10.1186/s13045-019-0724-7DOI Listing
April 2019
2 Reads

The Italian Rare Pancreatic Exocrine Cancer Initiative.

Tumori 2019 Apr 9:300891619839461. Epub 2019 Apr 9.

1 Medical Oncology Unit, IRCCS Cancer Institute "Giovanni Paolo II" of Bari, Bari, Italy.

Introduction: Exocrine pancreatic cancers include common type pancreatic ductal adenocarcinoma and cystic neoplasms, which account for 85% and 10% of cases, respectively. The remaining 5% are rare histotypes, comprising adenosquamous carcinoma, acinar cell carcinoma, signet ring cell carcinoma, medullary carcinoma, pancreatoblastoma, hepatoid carcinoma, undifferentiated carcinoma and its variant with osteoclast-like giant cells, solid pseudopapillary carcinoma, and carcinosarcoma. Due to their low incidence, little knowledge is available on their clinical and molecular features as well as on treatment choices. Read More

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http://dx.doi.org/10.1177/0300891619839461DOI Listing
April 2019
1 Read

Tumor-associated macrophages modulate resistance to oxaliplatin via inducing autophagy in hepatocellular carcinoma.

Cancer Cell Int 2019 25;19:71. Epub 2019 Mar 25.

1Department of Liver Surgery & Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032 China.

Background: Oxaliplatin-based chemotherapy is widely used to treat hepatocellular carcinoma (HCC). Recent studies suggested that therapeutic resistance of tumors was affected by tumor microenvironment (TME). As a major component of TME, the role of tumor-associated macrophages (TAMs) on drug resistance in HCC is largely unknown. Read More

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http://dx.doi.org/10.1186/s12935-019-0771-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434873PMC

MiR-9 promotes synovial sarcoma cell migration and invasion by directly targeting CDH1.

Int J Biochem Cell Biol 2019 Apr 5. Epub 2019 Apr 5.

Department of Orthopedics, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China. Electronic address:

Background: Invasion and metastasis of synovial sarcoma is the leading cause of death in patients. Epithelial mesenchymal transition (EMT) accelerates tumor cell invasion and metastasis. MiR-9 promotes tumor metastasis by inducing EMT. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.04.001DOI Listing

High expression of FMNL3 associates with cancer cell migration,invasion and unfavorable prognosis in tongue squamous cell carcinoma.

J Oral Pathol Med 2019 Apr 7. Epub 2019 Apr 7.

Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road 2, Guangzhou, Guangdong, China.

Background: The aim of this study was to ascertain the expression and role of FMNL3 in tongue squamous cell carcinoma (TSCC) tissues.

Materials And Methods: Immunohistochemistry, qRT-PCR and western blot were used to study the expression of FMNL3 in TSCC tissues and its adjacent normal tissues. The relationship between FMNL3 overexpression and various clinicopathological parameters and patients' survival were further assessed. Read More

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http://dx.doi.org/10.1111/jop.12857DOI Listing

Urine exosomal ceruloplasmin: a potential early biomarker of underlying kidney disease.

Clin Exp Nephrol 2019 Apr 6. Epub 2019 Apr 6.

Division of Nephrology, John H. Stroger, Jr. Hospital of Cook County, 1950 West Polk Street, Suites 5-56, Professional Building, Chicago, IL, 60612, USA.

Background: Previously we found that kidney tissue and urinary exosomes from patients of diabetic kidney disease showed high levels of ceruloplasmin (CP). Because CP is an acute-phase protein of kidney origin, it could be an early marker of many other kidney diseases. To investigate this hypothesis, we first measured urine exosomal and kidney expression of CP in non-diabetic chronic kidney disease (CKD) patients (membranous nephropathy, focal segmental glomerulosclerosis, lupus nephritis and IgA nephropathy) followed by a longitudinal study in rat passive Heymann nephritis (PHN), a model of human membranous nephropathy. Read More

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http://dx.doi.org/10.1007/s10157-019-01734-5DOI Listing
April 2019
1 Read

Effects of photobiomodulation therapy on the extracellular matrix of human dental pulp cell sheets.

J Photochem Photobiol B 2019 Apr 1;194:149-157. Epub 2019 Apr 1.

Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, Brazil. Electronic address:

Photobiomodulation therapy (PBMT) and the cell sheet (CS) technology improve processes relevant to tissue regeneration. The aim of this study was to investigate the effects of different PBMT parameters on the architecture (histology), protein composition (Western blotting and immunohistochemistry) and ultrastructure [scanning electron microscopy (SEM) and transmission electron microscopy (TEM)] of the extracellular matrix (ECM) synthesized by CSs composed by human dental pulp stem cells (hDPSCs).

Methods: Thawed cells were recharacterized by the expression profile of the surface molecules of mesenchymal stem cells (MSCs) using flow cytometry. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.017DOI Listing

Proteomic analysis and white spot syndrome virus interaction of mud crab (Scylla olivacea) revealed responsive roles of the hemocytes.

Fish Shellfish Immunol 2019 Apr 4. Epub 2019 Apr 4.

Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom, Thailand. Electronic address:

White spot disease (WSD) is a highly virulent viral disease in shrimps. Clinical signs and high mortality of WSD is generally observed after a few days of infection by White Spot Syndrome virus (WSSV). Mud crabs are the major carrier and persistent host for the WSSV. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193022
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http://dx.doi.org/10.1016/j.fsi.2019.03.070DOI Listing
April 2019
2 Reads

HER2 Positivity Predicts Unresponsiveness to EGFR-Targeted Treatment in Metastatic Colorectal Cancer.

Oncologist 2019 Apr 5. Epub 2019 Apr 5.

Niguarda Cancer Center, Grande Ospedale Metropolitano Niguarda, Milan, Italy

Background: HER2 amplification is detected in 3% of patients with colorectal cancer (CRC), making tumors in the metastatic setting vulnerable to double pharmacological HER2 blockade. Preclinical findings show that it also might impair response to anti-epidermal growth factor receptor (EGFR) treatment.

Subjects And Methods: Patients with exon 2 wild-type metastatic CRC underwent molecular screening of HER2 positivity by HERACLES criteria (immunohistochemistry 3+ or 2+ in ≥50% of cells, confirmed by fluorescence in situ hybridization). Read More

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http://dx.doi.org/10.1634/theoncologist.2018-0785DOI Listing
April 2019
2 Reads

IL6 Shapes an Inflammatory Microenvironment and Triggers the Development of Unique Types of Cancer in End-stage Kidney.

Anticancer Res 2019 Apr;39(4):1869-1874

Department of Urology, Medical School, University of Pecs, Pecs, Hungary

Background/aim: Chronic inflammation in end-stage kidney is associated with the development of pre-neoplastic lesions and renal cell tumors. The aim of this study was to clarify the role of the inflammatory microenvironment in this process.

Materials And Methods: We used representative microscopic slides from 11 end stage-kidneys containing pre-neoplastic lesions and tumors and applied immunohistochemistry to detect IL-6, SAA1 and LBP expression. Read More

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http://dx.doi.org/10.21873/anticanres.13294DOI Listing

Improvement in neurogenesis and memory function by administration of Passiflora incarnata L. extract applied to sleep disorder in rodent models.

J Chem Neuroanat 2019 Apr 2;98:27-40. Epub 2019 Apr 2.

Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan, 31538, Republic of Korea; Korea Mouse Phenotyping Center, Seoul, 08826, Republic of Korea. Electronic address:

Recently, there have been reports that chronic insomnia acts as an insult in the brain, causing memory loss through the production of ROS, inflammation, and, Alzheimer's disease if persistent. Insomnia remains the leading cause of sleep disturbance and as such has serious implications for public health. Patients with Alzheimer's disease are also known to suffer from severe sleep disturbance. Read More

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http://dx.doi.org/10.1016/j.jchemneu.2019.03.005DOI Listing
April 2019
6 Reads
2.520 Impact Factor

Our 2018 Cancer Cytopathology Young Investigator.

Authors:
William C Faquin

Cancer Cytopathol 2019 Apr;127(4):218-221

Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

Background: The Paris System for Urine Cytopathology (the Paris System) has succeeded in making the analysis of liquid-based urine preparations more reproducible. Any algorithm seeking to automate this system must accurately estimate the nuclear-to-cytoplasmic (N:C) ratio and produce a qualitative "atypia score." The authors propose a hybrid deep-learning and morphometric model that reliably automates the Paris System. Read More

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http://dx.doi.org/10.1002/cncy.22116DOI Listing

Promoting therapeutic angiogenesis of focal cerebral ischemia using thrombospondin-4 (TSP4) gene-modified bone marrow stromal cells (BMSCs) in a rat model.

J Transl Med 2019 Apr 4;17(1):111. Epub 2019 Apr 4.

Department of Biotherapy and Oncology, Shenzhen Luohu People's Hospital, Shenzhen, 518001, Guangdong, People's Republic of China.

Background: A stroke caused by angiostenosis always has a poor prognosis. Bone marrow stromal cells (BMSC) are widely applied in vascular regeneration. Recently, thrombospondin-4 (TSP4) was reported to promote the regeneration of blood vessels and enhance the function of endothelial cells in angiogenesis. Read More

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http://dx.doi.org/10.1186/s12967-019-1845-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449913PMC
April 2019
2 Reads
3.930 Impact Factor

[Observation of electroacupuncture intervention on cell apoptosis and Bax and Bcl-2 expression in cerebral and myocardial tissues in cerebral ischemia rats based on "heart-brain correlation" theory].

Zhen Ci Yan Jiu 2019 Feb;44(2):107-12

The First Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China.

Objective: To compare the effect of electroacupuncture (EA) of "Neiguan" (PC6) of the Pericardium Meridian, "Shenmen" (HT7) of the Heart Meridian,"Shuigou" (GV26) of the Governor Vessel and "Zhaohai" (KI6) of the Kidney Meridian on myocardial and cerebral cell apoptosis in cerebral ischemia (CI) rats, so as to explore its mechanism underlying improvement of CI based on the theory of "Heart-brain Correlation".

Methods: Forty-eight SD rats (half male and half female) were randomly divided into normal control, model, PC6, HT7, GV26 and KI6 groups (=8 in each one). The CI model was established by occlusion of the middle cerebral artery (MCAO). Read More

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http://dx.doi.org/10.13702/j.1000-0607.170332DOI Listing
February 2019
1 Read

Comparison of Intravoxel incoherent motion imaging and multiecho dynamic contrast-based MRI in rectal cancer.

J Magn Reson Imaging 2019 Apr 4. Epub 2019 Apr 4.

Department of Physics, University of Oslo, Oslo, Norway.

Background: Dynamic contrast-based MRI and intravoxel incoherent motion imaging (IVIM) MRI are both methods showing promise as diagnostic and prognostic tools in rectal cancer. Both methods aim at measuring perfusion-related parameters, but the relationship between them is unclear.

Purpose: To investigate the relationship between perfusion- and permeability-related parameters obtained by IVIM-MRI, T -weighted dynamic contrast-enhanced (DCE)-MRI and T *-weighted dynamic susceptibility contrast (DSC)-MRI. Read More

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http://dx.doi.org/10.1002/jmri.26740DOI Listing

Mus musculus deficient for secretory antibodies show delayed growth with an altered urinary metabolome.

Mol Med 2019 04 3;25(1):12. Epub 2019 Apr 3.

The Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Background: The polymeric immunoglobulin receptor (pIgR) maintains the integrity of epithelial barriers by transporting polymeric antibodies and antigens through the epithelial mucosa into the lumen. In this study, we examined the role of pIgR in maintaining gut barrier integrity, which is important for the normal development in mice.

Methods: Cohorts of pIgR mice and their wildtype controls were housed under Specific Pathogen Free (SPF) conditions and monitored for weight gain as an indicator of development over time. Read More

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http://dx.doi.org/10.1186/s10020-019-0077-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446318PMC

Qishen Yiqi Dropping Pills Ameliorates Doxorubicin-induced Cardiotoxicity in Mice via Enhancement of Cardiac Angiogenesis.

Med Sci Monit 2019 Apr 3;25:2435-2444. Epub 2019 Apr 3.

Department of Physical Examination Center, The Puren Hospital, Wuhan University of Science and Technology, Wuhan, Hubei, China (mainland).

BACKGROUND Qishen Yiqi Dropping Pills (QYDP) is a Chinese traditional medicine that has been applied to treat coronary heart disease and ischemic heart failure in China. However, few studies have explored whether QYDP exerted an effect on doxorubicin (Doxo)-induced cardiotoxicity. Hence, in this study we investigated the effect of QYDP on cardiotoxicity induced by doxorubicin (Doxo) and its potential mechanism. Read More

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http://dx.doi.org/10.12659/MSM.915194DOI Listing
April 2019
4 Reads

Cyclin D1 and p57 expression in relation to clinicopathological characteristics and overall survival in patients with renal cell carcinoma.

J BUON 2019 Jan-Feb;24(1):301-309

Institute of Pathology, School of Medicine, University of Belgrade, Belgrade, Serbia.

Purpose: There is a need for identifying molecular prognostic biomarkers to better predict clinical outcomes in patients with renal cell carcinoma (RCC). This study investigated the pattern of cyclin D1 and p57 expression in RCC patients and evaluated their relation with clinicopathological characteristics and overall survival (OS).

Methods: Immunohistochemistry was applied to paraffin-embedded tissue sections of 74 RCC patients. Read More

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April 2019
1 Read
0.706 Impact Factor

Utility of ankyrin 3 as a prognostic marker in androgen-receptor-positive breast cancer.

Breast Cancer Res Treat 2019 Apr 2. Epub 2019 Apr 2.

Division of Cancer and Stem Cells, Nottingham Breast Cancer Research Centre, School of Medicine, University of Nottingham, Nottingham, UK.

Purpose: Androgen receptor (AR) and AR signaling pathways are thought to play a role in breast cancer (BC) and are potentially related to treatment responses and outcomes. Ankyrin 3 (ANK3) is associated with AR stability in cancer cells. In the present study, we investigated the clinicopathological utility of ANK3 expression with emphasis on AR and its associated signalling pathway at transcriptomic and proteomic phases. Read More

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http://dx.doi.org/10.1007/s10549-019-05216-wDOI Listing
April 2019
5 Reads
3.940 Impact Factor

Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis.

PLoS One 2019 2;14(4):e0214709. Epub 2019 Apr 2.

Department for Companion Animals and Horses, University Equine Hospital, Equine Surgery, University of Veterinary Medicine, Vienna, Austria.

The purpose of the current study was to establish an in vitro model for osteoarthritis (OA) by co-culture of osteochondral and synovial membrane explants. Osteochondral explants were cultured alone (control-1) or in co-culture with synovial membrane explants (control-2) in standard culture medium or with interleukin-1β (IL1β) and tumor necrosis factor (TNFα) added to the culture medium (OA-model-1 = osteochondral explant; OA-model-2 = osteochondroal-synovial explant). In addition, in OA-model groups a 2-mm partial-thickness defect was created in the centre of the cartilage explant. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214709PLOS

A novel optical tracer for VMAT2 applied to live cell measurements of vesicle maturation in cultured human β-cells.

Sci Rep 2019 Apr 1;9(1):5403. Epub 2019 Apr 1.

Division of Endocrinology, Department of Medicine and Naomi Berrie Diabetes Center, Columbia University Medical Centre, New York, New York, USA.

The islet β-cells integrate external signals to modulate insulin secretion to better regulate blood glucose levels during periods of changing metabolic demand. The vesicular monoamine transporter type 2 (VMAT2), an important regulator of CNS neurotransmission, has an analogous role in the endocrine pancreas as a key control point of insulin secretion, with additional roles in regulating β-cell differentiation and proliferation. Here we report on the synthesis and biological characterisation of a fluorescent ligand for VMAT2 suitable for live cell imaging. Read More

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http://dx.doi.org/10.1038/s41598-019-41891-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443945PMC
April 2019
5.078 Impact Factor

[Therapeutic effect of dihydroartemisinin on pulmonary fibrosis in rats with dust].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2019 Feb;37(2):96-103

School of Medicine and Life Science, Universicy of Jinan-Shandong Academy of Medicine Sciences, Jinan 250062, China; Shandong Academy of Medical Sciences Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China.

To observe the therapeutic effect of dihydroartemisinin on pulmonary fibrosis in rats exposed to dust, and compare the therapeutic effects of dihydroartemisinin and tetrandrine. Sixty male Sprague-Dawley rats were randomly divided into control group, model group, treatment group 1 and treatment group 2, with 15 rats in each group. The model group and the treatment group were stained with disposable non-exposure silica tracheal instillation method. Read More

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http://dx.doi.org/10.3760/cma.j.issn.1001-9391.2019.02.003DOI Listing
February 2019

Review of ependymomas: assessment of consensus in pathological diagnosis and correlations with genetic profiles and outcome.

Brain Tumor Pathol 2019 Mar 30. Epub 2019 Mar 30.

Department of Neuro-Oncology/Neurosurgery, Saitama Medical University International Medical Center, Saitama, Japan.

We focused on histological and immunohistochemical characteristics of ependymoma (EPN) with molecular profiles to develop more reproducible criteria of the diagnosis. Three expert neuropathologists reviewed the pathology of 130 samples from the Japan Pediatric Molecular Neuro-Oncology Group study. Confirmed cases were assessed for histology, surrogate markers, molecular subgrouping, and survival data. Read More

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http://dx.doi.org/10.1007/s10014-019-00338-xDOI Listing
March 2019
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Immunohistochemistry for Anti-diabetes Drug, Alogliptin Using a Newly Prepared Monoclonal Antibody: Its Precise Localization in Rat Small Intestine.

Acta Histochem Cytochem 2019 Feb 23;52(1):27-34. Epub 2019 Feb 23.

Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.

Knowledge of time sequence of localization of drugs in cells and tissues of animals may help in developing a better understanding of the actual overall pharmacokinetics of the drugs. We produced monoclonal antibody (mAb) against alogliptin (AG), a dipeptidyl peptidase-4 (DPP-4) inhibitor, conjugated to BSA with -(γ-maleimidobutyryloxy)-succinimide. The mAb was specific for AG and did not cross-react with sitagliptin, vancomycin or amoxicillin. Read More

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http://dx.doi.org/10.1267/ahc.18036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434317PMC
February 2019

Parafibromin immunostainings of parathyroid tumors in clinical routine: a near-decade experience from a tertiary center.

Mod Pathol 2019 Mar 28. Epub 2019 Mar 28.

Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.

The cell division cycle 73 gene is mutated in familial and sporadic forms of primary hyperparathyroidism, and the corresponding protein product parafibromin has been proposed as an adjunct immunohistochemical marker for the identification of cell division cycle 73 mutations and parathyroid carcinoma. Here, we present data from our experiences using parafibromin immunohistochemistry in parathyroid tumors since the marker was implemented in clinical routine in 2010. A total of 2019 parathyroid adenomas, atypical adenomas, and carcinomas were diagnosed in our department, and parafibromin staining was ordered for 297 cases with an initial suspicion of malignant potential to avoid excessive numbers of false positives. Read More

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http://dx.doi.org/10.1038/s41379-019-0252-6DOI Listing
March 2019
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The role of podocyte damage in the etiology of ischemia-reperfusion acute kidney injury and post-injury fibrosis.

BMC Nephrol 2019 Mar 28;20(1):106. Epub 2019 Mar 28.

Department of Nephrology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

Background: To establish a model of chronic renal fibrosis following acute kidney injury (AKI) in BALB/c mice and to observe the effect of AKI on podocyte injury and chronic fibrosis of the kidney. Additional aims included using the model to explore the role of podocyte injury in AKI and post-injury fibrosis.

Methods: Fifty BALB/C mice were randomly divided into control group (Ctr), sham group (sham), AKI 20 group (renal ischemia, 20 min reperfusion), AKI 30 group (renal ischemia, 30 min reperfusion) and AKI 40 group (renal ischemia, 40 min reperfusion). Read More

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http://dx.doi.org/10.1186/s12882-019-1298-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438002PMC
March 2019
1 Read

Tuberculosis Surveillance in Taiwan Forensic Autopsy Cases: A Retrospective Analysis of 71 Cases From 2012 to 2017.

Am J Forensic Med Pathol 2019 Mar 23. Epub 2019 Mar 23.

Tuberculosis (TB) is one of the most important public health issues worldwide, and global efforts have altered the TB epidemic. This study analyzed 71 cases of TB at autopsy notified via Taiwan Medical Examiner Surveillance for Lethal Infectious Disease (Taiwan Med-X) between 2012 and 2017 and applied immunohistochemistry to formalin-fixed lung tissue. Tuberculosis was present in 0. Read More

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http://dx.doi.org/10.1097/PAF.0000000000000477DOI Listing
March 2019
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Role of SIRT-3, p-mTOR and HIF-1α in Hepatocellular Carcinoma Patients Affected by Metabolic Dysfunctions and in Chronic Treatment with Metformin.

Int J Mol Sci 2019 Mar 26;20(6). Epub 2019 Mar 26.

Division of Medical Oncology, Department of Medical and Surgical Sciences for Children and Adults, University Hospital of Modena, 41122 Modena, Italy.

The incidence of hepatocellular carcinoma deriving from metabolic dysfunctions has increased in the last years. Sirtuin- (SIRT-3), phospho-mammalian target of rapamycin (p-mTOR) and hypoxia-inducible factor- (HIF-1α) are involved in metabolism and cancer. However, their role in hepatocellular carcinoma (HCC) metabolism, drug resistance and progression remains unclear. Read More

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http://dx.doi.org/10.3390/ijms20061503DOI Listing

TRIM2 is a novel promoter of human colorectal cancer.

Scand J Gastroenterol 2019 Mar 27:1-9. Epub 2019 Mar 27.

d Precise Genome Engineering Center, School of Life Sciences , Guangzhou University , Guangzhou , China.

Objectives: The incidence of colorectal cancer (CRC) is increasing year by year and appears to be younger, due to the low early diagnosis rate and metastasis. It is difficult to remedy by conventional treatment. Here, we reported that tripartite motif containing protein 2 (TRIM2) could promote tumor growth, invasion and metastasis of CRC via a mechanism that involved EMT both in vitro and in vivo. Read More

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http://dx.doi.org/10.1080/00365521.2019.1575463DOI Listing

A Mediterranean Diet Mix Has Chemopreventive Effects in a Murine Model of Colorectal Cancer Modulating Apoptosis and the Gut Microbiota.

Front Oncol 2019 12;9:140. Epub 2019 Mar 12.

Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.

Unhealthy dietary patterns have been associated with colorectal cancer (CRC) onset while Mediterranean Diet (MD) has been proposed for CRC prevention. This study evaluated the effect of a Mediterranean Diet Mix (MD-MIX) on colonic tumors development in A/J mice fed a low-fat (LFD) or a high-fat western diet (HFWD), and injected with the procarcinogen azoxymethane (AOM). Forty A/J male mice were randomly assigned into four feeding arms (10 mice/arm; LFD, LFD-MD-MIX, HFWD, HFWD-MD-MIX) to be treated with AOM. Read More

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http://dx.doi.org/10.3389/fonc.2019.00140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423077PMC

Transcription regulators are transiently expressed during the prostate gland adaptation to the hypoandrogenic environment.

Histol Histopathol 2019 Mar 26:18105. Epub 2019 Mar 26.

Department of Structural and Functional Biology, Institute of Biology, Institute of Physics Gleb Wataghin, State University of Campinas (UNICAMP), Campinas SP, Brazil.

The high incidence of prostatic diseases, including malignant tumors, makes the understanding of prostate biology very important. Androgen deprivation, blockade by orchiectomy, or chemical castration causes prostate and tumor shrinkage. The gene networks involved in a cell type-specific fashion are rather unknown. Read More

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http://dx.doi.org/10.14670/HH-18-105DOI Listing