1,289,207 results match your criteria Applied Immunohistochemistry & Molecular Morphology[Journal]


Research ethics and public trust in vaccines: the case of COVID-19 challenge trials.

Authors:
Nir Eyal

J Med Ethics 2022 May 20. Epub 2022 May 20.

Center for Population-Level Bioethics, Department of Philosophy (SAS) and Department of HBSP (SPH), Rutgers University, New Brunswick, New Jersey, USA

Despite their clearly demonstrated safety and effectiveness, approved vaccines against COVID-19 are commonly mistrusted. Nations should find and implement effective ways to boost vaccine confidence. But the implications for ethical vaccine development are less straightforward than some have assumed. Read More

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[Physiotherapy in German Neonatal Intensive Care Units - Indication and Clinical Application of the General Movements Assessments].

Z Geburtshilfe Neonatol 2022 May 20. Epub 2022 May 20.

Klinik für Kinderheilkunde I, Neonatologie, Pädiatrische Intensivmedizin, Pädiatrische Neurologie, Universitätsmedizin Essen, Essen, Deutschland.

Background: Standardized recommendations for inpatient and outpatient physiotherapy for preterm infants are lacking in Germany. The aim of this study was to investigate the prescription practice in German neonatal intensive care units. Are standardized assessments used to determine indications? How important is the General Movements Assessment in postnatal diagnostics?

Methods: We conducted an online survey in German neonatal intensive care units asking 21 questions on indication and prescription practices for inpatient and outpatient physiotherapy in preterm infants' care as well as on General Movements Assessment and its clinical application via Lime Survey. Read More

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Optimization of Sporulation and Purification Methods for Sporicidal Efficacy Assessment on Bacillus Spores.

J Ind Microbiol Biotechnol 2022 May 20. Epub 2022 May 20.

Division of Microbiology, National Center for Toxicological Research, U.S. FDA, Jefferson, AR, USA.

Validating the efficacy of sporicidal agents is a critical step in current good manufacturing practices for disinfection requirements. A limitation is that the poor quality of spores can lead to false positive sporicidal results. The aim of this study was to explore optimal sporulation and purification methods in Bacillus spores. Read More

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[Documentation, Application and Efforts to Reduce Involuntary Admissions and Coercive Measures - Results from a Nationwide Study on Acute Psychiatric Hospitals in Germany].

Psychiatr Prax 2022 May 20. Epub 2022 May 20.

LWL-Universitätsklinikum Bochum der Ruhr-Universität Bochum, Klinik für Psychiatrie, Psychotherapie und Präventivmedizin.

Objective: This nationwide full census survey investigated the documentation status regarding involuntary admissions and coercive measures in psychiatric hospitals in Germany.

Methods: As part of the ZIPHER study, a questionnaire survey on the documentation, application and reduction of coercion was conducted ( = 147, response rate = 34.4 %). Read More

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The three-year evolution of overactive bladder syndrome in community-dwelling female residents aged 40 years and above.

Taiwan J Obstet Gynecol 2022 May;61(3):479-484

Department of Obstetrics and Gynecology, St. Joseph's Hospital, Yunlin County, Taiwan. Electronic address:

Objective: In this 3-year longitudinal cohort study, we aimed to evaluate the evolution of overactive bladder in female community residents aged 40 years and above in central Taiwan and identify its risk factors.

Materials And Methods: Female community residents aged 40 years and above were invited to participate in this study and fill out a yearly Overactive Bladder Symptom Score (OABSS) questionnaire over a 3-year period. A woman was defined to have OAB if the total OABSS was ≧4 and urgency score was ≧2. Read More

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Relationship between extreme values of first trimester maternal pregnancy associated plasma Protein-A, free-β-human chorionic gonadotropin, nuchal translucency and adverse pregnancy outcomes.

Taiwan J Obstet Gynecol 2022 May;61(3):433-440

Obstetrics and Gynecology Department Okmeydanı Training and Research Hospital Istanbul, Turkey (Prof. Dr. Cemil Tascıoglu City Hospital), Darulaceze Cad. No:25, Okmeydani, Sisli, 34384, Istanbul, Turkey. Electronic address:

Objective: The aim of our study was to investigate the relationship between extreme values of first trimester screening markers and adverse obstetric outcomes.

Materials And Methods: Our study was conducted by examining the prenatal and postnatal perinatal records of 786 singleton gestations between the ages of 18-40, who applied to Prof. Dr. Read More

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Towards the creation of personalized bakery products using 3D food printing.

Adv Food Nutr Res 2022 28;99:1-35. Epub 2021 Dec 28.

Equipment for Additive Manufacturing Department, Netherlands Organisation for Applied Scientific Research (TNO), Eindhoven, The Netherlands.

Bakery products with interesting color, shape and texture have been created using 3D food printing. Current research focuses on the development of new formulations and the optimization of the printing and post-printing treatment processes, in order to obtain high-quality 3D-printed bakery products. Knowledge about food rheology is useful for the development of dough formulations with good 3D-printability. Read More

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December 2021

Antifouling ionic liquid doped molecularly imprinted polymer-based ratiometric electrochemical sensor for highly stable and selective detection of zearalenone.

Anal Chim Acta 2022 Jun 30;1210:339884. Epub 2022 Apr 30.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China. Electronic address:

Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin, and its accurate detection in complex biological samples is still a challenge. Herein, an antifouling ratiometric electrochemical sensor has been developed for its detection. In this system, black phosphorus-graphene oxide is used as substrate to amplify the signal; ionic liquid doped molecularly imprinted polymer (MIP) endows the sensing surface not only high recognition ability but also high capability to resist nonspecific adsorption. Read More

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pH-guided fluorescent sensing probe for the discriminative detection of Cl and Br in human serum.

Anal Chim Acta 2022 Jun 27;1210:339879. Epub 2022 Apr 27.

Department of Chemistry, Gwangju Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, Republic of Korea. Electronic address:

The fluctuation of the halide ion concentration in human serum is of a high clinical implication. The measurement of Cl in human serum samples is important because Cl is a major constituent of human serum and certain Cl levels are indicators of many acute diseases. Real-time monitoring of Br levels in human serum samples from patients ingesting Br salt-based antiepileptic drugs is important to regulate potential adverse drug effects. Read More

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A novel UHPLC-ESI-MS/MS method and automatic calculation software for regiospecific analysis of triacylglycerols in natural fats and oils.

Anal Chim Acta 2022 Jun 1;1210:339887. Epub 2022 May 1.

Food Chemistry and Food Development, Department of Life Technologies, FI-20500, University of Turku, Turku, Finland. Electronic address:

Regioisomeric analysis of triacylglycerols (TAGs) in natural oils and fats is a highly challenging task in analytical chemistry. Here we present a software (TAG Analyzer) for automatic calculation of regioisomeric composition of TAGs based on the mass spectral data from recently reported ultra-high performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method for analyzing TAG regioisomers. The software enables fast and accurate processing of complex product ion spectra containing structurally informative diacylglycerol [M+NH-RCOH-NH] and fatty acid ketene [RCO] fragment ions. Read More

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Trapped ion mobility spectrometry-mass spectrometry improves the coverage and accuracy of four-dimensional untargeted lipidomics.

Anal Chim Acta 2022 Jun 2;1210:339886. Epub 2022 May 2.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, PR China. Electronic address:

Lipids play vital roles in many physiological and pathological processes in living organisms. Due to the high structural diversity and the numerous isomers and isobars of lipids, high-coverage and high-accuracy lipidomic analysis of complex biological samples remain the bottleneck to investigate lipid metabolism. Here, we developed the trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) based four-dimensional untargeted lipidomics to support accurate lipid identification and quantification in biological samples. Read More

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Renewable sorbent dispersive solid phase extraction automated by Lab-In-Syringe using magnetite-functionalized hydrophilic-lipophilic balanced sorbent coupled online to HPLC for determination of surface water contaminants.

Anal Chim Acta 2022 Jun 26;1210:339874. Epub 2022 Apr 26.

Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Hradec Králové, 500 05, Czech Republic. Electronic address:

An automated methodology for magnetic dispersive solid phase microextraction integrating bead injection approach for renewable sorbent introduction is presented for the first time and was successfully applied to the enrichment of water contaminants. For this purpose, a simple procedure was developed for the functionalization of commercial SupelTM-Select HLB (Hydrophilic modified styrene polymer) sorbent beads that allowed embedding magnetite nanoparticles (FeO). The sorbent was then used in a dispersive solid phase extraction procedure that was carried out entirely inside the void of an automatic syringe pump following the flow-batch concept of Lab-In-Syringe including automated renewal of the sorbent for each analysis. Read More

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A scoping review of distributed ledger technology in genomics: thematic analysis and directions for future research.

J Am Med Inform Assoc 2022 May 20. Epub 2022 May 20.

Department of Economics and Management, Karlsruhe Institute of Technology, Karlsruhe, Germany.

Objective: Rising interests in distributed ledger technology (DLT) and genomics have sparked various interdisciplinary research streams with a proliferating number of scattered publications investigating the application of DLT in genomics. This review aims to uncover the current state of research on DLT in genomics, in terms of focal research themes and directions for future research.

Materials And Methods: We conducted a scoping review and thematic analysis. Read More

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3D electron diffraction for structure determination of small-molecule nanocrystals: A possible breakthrough for the pharmaceutical industry.

Wiley Interdiscip Rev Nanomed Nanobiotechnol 2022 May 20:e1810. Epub 2022 May 20.

Center for Materials Interfaces, Electron Crystallography, Istituto Italiano di Tecnologia, Pontedera.

Nanomedicine is among the most fascinating areas of research. Most of the newly discovered pharmaceutical polymorphs, as well as many new synthesized or isolated natural products, appear only in form of nanocrystals. The development of techniques that allow investigating the atomic structure of nanocrystalline materials is therefore one of the most important frontiers of crystallography. Read More

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In silico-in vitro estimation of lipophilicity and permeability association for succinimide derivatives using chromatographic anisotropic systems and parallel artificial membrane permeability assay (PAMPA).

Biomed Chromatogr 2022 May 20. Epub 2022 May 20.

University of Novi Sad, Faculty of Medicine, Department of Pharmacy, Novi Sad, Serbia.

Passive permeability is one of the key features that determines absorbability and one of the most studied properties in the early phases of drug development. Newly synthesized succinimide derivatives from two different series (1-aryl-3-methylsuccinimides and 1-aryl-3-ethyl-3-methylsuccinimides) with high biological potential have been subjected to estimation of their passive permeability and its association with i) experimentally obtained anisotropic lipophilicity, ii) in silico calculated lipophilicity and iii) in silico predicted permeability and absorbability. Non-cellular based parallel artificial membrane permeability assay (PAMPA) was applied for quantifying their passive permeation, expressed as logPapp. Read More

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A chromosome-level genome assembly of the pollinating fig wasp Valisia javana.

DNA Res 2022 May 20. Epub 2022 May 20.

Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China.

Fig wasp has always been thought the species-specific pollinator for their host fig (Moraceae, Ficus) and constitute a model system with its host to study co-evolution and co-speciation. The availability of a high-quality genome will help to further reveal the mechanisms underlying these characteristics. Here we present a high-quality chromosome-level genome for V. Read More

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Aboveground Plant-to-Plant Electrical Signaling Mediates Network Acquired Acclimation.

Plant Cell 2022 May 20. Epub 2022 May 20.

Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.

Systemic acquired acclimation and wound signaling require the transmission of electrical, calcium and reactive oxygen species (ROS) signals between local and systemic tissues of the same plant. However, whether such signals can be transmitted between two different plants is largely unknown. Here, we reveal a new type of plant-to-plant aboveground direct communication involving electrical signaling detected at the surface of leaves, ROS and photosystem networks. Read More

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Marsdenia tenacissima (Roxb.) Moon injection exerts a potential anti-tumor effect in prostate cancer through inhibiting ErbB2-GSK3β-HIF1α signaling axis.

J Ethnopharmacol 2022 May 17:115381. Epub 2022 May 17.

Guangxi Key Laboratory for Bioactive Molecules Research and Evaluation & College of Pharmacy, Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, China; Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education & Center for Translational Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, China. Electronic address:

Ethnopharmacological Relevance: Marsdenia tenacissima injection (MTE), a traditional Chinese medical injection extracted from the rattan of Marsdenia tenacissima (Roxb.) Moon, has been approved for clinical use in China as an adjuvant therapeutic agent in multiple cancers, including esophageal cancer, gastric cancer, lung cancer, and liver cancer. However, the activity and mechanism of MTE on prostate cancer (PCa) remain to be defined. Read More

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Impact of "Defensive Medicine" On the Costs of Diabetes and Associated Conditions.

Ann Vasc Surg 2022 May 17. Epub 2022 May 17.

Department of Vascular Surgery, Mayo Clinic Arizona, 5777 E Mayo Blvd Phoenix, AZ 85054.

Objectives: Geographic variation in healthcare spending is typically attributed to differences in patient health status and provider practice patterns. While medicolegal considerations (i.e. Read More

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Association between carotid atherosclerosis and brain activation patterns during the Stroop task in older adults: an fNIRS investigation.

Neuroimage 2022 May 17:119302. Epub 2022 May 17.

MRC Unit for Lifelong Health and Ageing at University College London, Department of Population Science and Experimental Medicine, Institute of Cardiovascular Science, 1-19 Torrington Place, London, WC1E 7HB, United Kingdom. Electronic address:

There is an increasing body of evidence suggesting that vascular disease could contribute to cognitive decline and overt dementia. Of particular interest is atherosclerosis, as it is not only associated with dementia, but could be a potential mechanism through which cardiovascular disease directly impacts brain health. In this work, we evaluated the differences in functional near infrared spectroscopy (fNIRS)-based measures of brain activation, task performance, and the change in central hemodynamics (mean arterial pressure (MAP) and heart rate (HR)) during a Stroop color-word task in individuals with atherosclerosis, defined as bilateral carotid plaques (n = 33) and healthy age-matched controls (n = 33). Read More

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Living wearables: Bacterial reactive glove.

Biosystems 2022 May 17:104691. Epub 2022 May 17.

Unconventional Computing Laboratory, University of the West England, Bristol, UK.

A reactive bacterial glove is a cotton glove colonised by Acetobacter aceti, an example of biofabrication of a living electronic sensing device. The bacterial colony, supported by a cellulose-based hydrogel, forms a several millimetres-thick living coating on the surface of the glove. This paper proposes a novel method for analysing the complex electrical activity of trains of spikes generated by a living colony. Read More

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Z-scan optical method complements the Thioflavin T assay for investigation of anti-Alzheimer's impact of polyphenols.

Photodiagnosis Photodyn Ther 2022 May 17:102914. Epub 2022 May 17.

Biophotonics Lab, Faculty of Physics, Kharazmi University, Karaj 31979-37551, Iran; Applied Science Research Center, Kharazmi University, Karaj 31979-37551, Iran.

Polyphenols are tremendously effective in eliminating the amyloid-beta aggregations, the main hallmark of Alzheimer's disease. In recent years various nano drugs and biomaterials based on polyphenolic compounds have been synthetized to treat or prevent Alzheimer's disease, and the main in-vitro approach to investigate the anti-Alzheimer's properties of materials, is Thioflavin T assay. In spite of being very helpful, it has some drawbacks and cannot guarantee the accuracy of data, specifically in case of polyphenolic compounds; thus, rendering accurate results requires utilizing other assays along with Thioflavin T. Read More

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What have we learned from genome-wide association studies (GWAS) in Parkinson disease?

Ageing Res Rev 2022 May 17:101648. Epub 2022 May 17.

Centre for Genetic Epidemiology, Institute for Clinical Epidemiology and Applied Biometry, University of Tübingen, DE-72076 Tübingen, Germany. Electronic address:

After fifteen years of genome-wide association studies (GWAS) in Parkinson's disease (PD), what have we learned? Addressing this question will help catalogue the progress made towards elucidating disease mechanisms, improving the clinical utility of the identified loci, and envisioning how we can harness the strides to develop translational GWAS strategies. Here we review the advances of PD GWAS made to date while critically addressing the challenges and opportunities for next-generation GWAS. Thus, deciphering the missing heritability in underrepresented populations is currently at the reach of hand for a truly comprehensive understanding of the genetics of PD across the different ethnicities. Read More

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Micelle Incorporated Liquid-Chromatography in the Light of Green Chemistry: an Application for the Quality Control Analysis of anti-Platelet Fixed-Dose Combinations.

J AOAC Int 2022 May 20. Epub 2022 May 20.

Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, P.C.44519 Zagazig, Egypt.

Background: Oral anti-platelet agents are the cornerstone of treatment of multiple cardiovascular diseases and in the long-term prevention of their recurrence.

Objective: In the present work, we report a method based on micellar liquid chromatography coupled with ultraviolet detection (MLC/UV), for the simultaneous quantification of combined anti-platelet therapy namely, clopidogrel bisulphate (CPS), aspirin (ASP), together with salicylic acid (SA), in their pharmaceutical dosage form.

Methods: The incorporation of 0. Read More

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Structural changes in aptamers are essential for synthetic riboswitch engineering.

J Mol Biol 2022 May 17:167631. Epub 2022 May 17.

Department of Biology, Technical University of Darmstadt, Schnittspahnstraße 10, D-64287 Darmstadt, Germany; Center for Synthetic Biology, Technical University of Darmstadt. Electronic address:

Synthetic riboswitches are powerful tools in synthetic biology in which sensing and execution are consolidated in a single RNA molecule. By using SELEX to select aptamers in vitro, synthetic riboswitches can in theory be engineered against any ligand of choice. Surprisingly, very few in vitro selected aptamers have been used for the engineering of synthetic riboswitches. Read More

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Removal of antibiotics from wastewaters by membrane technology: Limitations, successes, and future improvements.

Sci Total Environ 2022 May 16:156010. Epub 2022 May 16.

Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran; Department of Environmental Engineering, Gebze Technical University, 41400 Gebze, Turkey.

Antibiotics and related pharmaceuticals are applied to enhance public health and life quality. A major environmental concern is wastewaters from pharmaceutical industries, which contain significant amounts of antibiotics. Pharmaceutical industries apply conventional processes (biological, filtration, coagulation, flocculation, and sedimentation) for wastewater treatment, but these approaches cannot remove antibiotics completely. Read More

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