8 results match your criteria Applied Biochemistry And Microbiology[Journal]

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Synthesis of a Bioactive Composition of Chitosan-Selenium Nanoparticles.

Appl Biochem Microbiol 2022 24;58(2):126-131. Epub 2022 Mar 24.

National Research Lobachevsky State University of Nizhny Novgorod, 603022 Nizhny Novgorod, Russia.

A biologically active composition of chitosan-selenium nanoparticles has been developed. Selenium nanoparticles are characterized by a clear bimodal size distribution: 2-3 and ~37 nm. The main active centers of complexation with nanoparticles are the amino and hydroxyl groups of chitosan. Read More

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VKM Aс-2618D-an Effective Producer of Tacrolimus.

Appl Biochem Microbiol 2021 14;57(9):939-948. Epub 2021 Dec 14.

Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow oblast Russia.

The sp. VKM Ac-2618D strain has been identified, and its morphological and physiological features have been studied in relation to the production of the immunosuppressant tacrolimus. The phenotypic variability of the strain was analyzed, and a dissociant with a high level of tacrolimus production was selected. Read More

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December 2021

Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics.

Appl Biochem Microbiol 2021 14;57(8):845-850. Epub 2021 Dec 14.

Filatov City Clinical Hospital no. 15, Department of Health of Moscow, 111539 Moscow, Russia.

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. Read More

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December 2021

CRISPR Interference of Adenylate Cyclases from .

Appl Biochem Microbiol 2021 27;57(4):421-425. Epub 2021 Jul 27.

Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071 Moscow, Russia.

This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use of genes of adenylate cyclases. Read More

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Use of Mathematical Methods for the Biosafety Assessment of Agricultural Crops.

Appl Biochem Microbiol 2021 12;57(2):271-279. Epub 2021 Mar 12.

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.

In Russia and around the world, there are important questions regarding the potential threats to national and biological safety created by genetic technologies and the need to improve or introduce new, justified, and adequate measures for their control, regulation, and prevention. The article shows that a significant volume of the global market is occupied by five major transgenic crops, and producers are ready to switch to crops with an edited genome that has been approved in the United States, Argentina, and other countries. We propose a qualitatively new approach to the risk assessment of edited plants, "Safe Design," and we have also developed an extremely important, fundamentally new approach to the development of methods that combine next-generation sequencing (NGS) and Bioinformatics for the assessment of the crop import biosafety. Read More

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Vaccines Against Tuberculosis: Problems and Prospects (Review).

Appl Biochem Microbiol 2020 22;56(5):497-504. Epub 2020 Sep 22.

Bach Institute of Biochemistry, Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.

Despite the efforts of the global medical and scientific community, tuberculosis remains the leading cause of death from infectious diseases. The expectation of success associated with the development of new anti-TB drugs was not justified, and the attention of researchers was largely drawn to the creation of new mycobacterial strains for vaccination against tuberculosis. The proposed review contains current information on the existing vaccine strains and the development of new, genetically engineered strains for the prevention of tuberculosis and the prevention and treatment of other diseases. Read More

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September 2020

Plant Based Bioreactors of Recombinant Cytokines (Review).

Appl Biochem Microbiol 2016 31;52(2):121-137. Epub 2016 Mar 31.

Biology Faculty, St. Petersburg State University, St. Petersburg, 199034 Russia.

Cytokines are a family of signaling polypeptides involved in intercellular interactions in the process of the immune response, as well as in the regulation of a number of normal physiological functions. Cytokines are used in medicine for the treatment of cancer, immune disorders, viral infections, and other socially significant diseases, but the extent of their use is limited by the high production cost of the active agent. The development of this area of pharmacology is associated with the success of genetic engineering, which allows the production of significant amounts of protein by transgenic organisms. Read More

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Progress in the development of pandemic influenza vaccines and their production technologies.

O I Kiselev

Appl Biochem Microbiol 2010 12;46(9):815-830. Epub 2010 Nov 12.

Research Institute of Influenza, North Western Division, Russian Academy of Medical Sciences (RAMS), St. Petersburg, 197376 Russia.

This article analyzes the current situation in the field of construction and production of pandemic influenza vaccines. The main task of protecting the population against influenza pandemics requires state-of-the-art approaches to the construction of influenza vaccines to be based on reassortment and genetic engineering techniques, including the analysis of primary structures of influenza viral genes, synthesis and cloning of the main viral genes, reverse genetics techniques, and banks of plasmids bearing basic viral genes. Reassortant technologies are now giving way to new approaches for objective reasons. Read More

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November 2010
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