957,337 results match your criteria Applied & translational genomics[Journal]


Oxygen Saturation and Perfusion Index-Based Enhanced Critical Congenital Heart Disease Screening.

Am J Perinatol 2019 Apr 19. Epub 2019 Apr 19.

Department of Pediatrics, University of California, Davis, Sacramento, California.

Objective:  To determine if addition of perfusion index (PIx) to oxygen saturation (SpO) screening improves detection of critical congenital heart disease (CCHD) with systemic outflow obstruction.

Study Design:  We determined screening thresholds for PIx and applied these to a cohort of newborns with and without congenital heart disease (CHD).

Results:  A total of 123 normal and 21 CHD newborns (including five with critical systemic outflow obstruction) were enrolled. Read More

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http://dx.doi.org/10.1055/s-0039-1685445DOI Listing

Arachnoid web of the spine: a systematic literature review.

J Neurosurg Spine 2019 Apr 19:1-10. Epub 2019 Apr 19.

2Department of Neurosurgery, Weill Cornell Brain and Spine Center, New York, New York.

OBJECTIVEAn arachnoid web of the spine (AWS) is a rare and oftentimes challenging lesion to diagnose, given its subtle radiographic findings. However, when left untreated, this lesion can have devastating effects on a patient's neurological function. To date, only limited case reports and series have been published on this topic. Read More

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http://dx.doi.org/10.3171/2019.1.SPINE181371DOI Listing

MicroRNA-181b-5p attenuates early postoperative cognitive dysfunction by suppressing hippocampal neuroinflammation in mice.

Cytokine 2019 Apr 16;120:41-53. Epub 2019 Apr 16.

Department of Anesthesiology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan, Hubei, China. Electronic address:

Background: Postoperative cognitive dysfunction (POCD) is a common complication after surgery and its occurrence is associated with increased morbidity and mortality. However, the pathophysiology of this complication remains largely unknown. Efforts to identify causes of POCD have focused on the hippocampal neuroinflammation. Read More

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http://dx.doi.org/10.1016/j.cyto.2019.04.005DOI Listing

Directional fast-marching and multi-model strategy to extract coronary artery centerlines.

Comput Biol Med 2019 Apr 5;108:67-77. Epub 2019 Apr 5.

School of Data Science, Fudan University, Shanghai, China. Electronic address:

Background: Computed tomography angiography (CTA) is a non-invasive technique to image coronary arteries and evaluate coronary artery diseases (CAD). The diagnosis of CAD requires modeling anatomical structures and analyzing the function and pathology of the coronary arteries. Therefore, a robust and automated method for extracting reliable coronary artery centerlines is valuable in clinical practice. Read More

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http://dx.doi.org/10.1016/j.compbiomed.2019.03.029DOI Listing

DeepClas4Bio: Connecting bioimaging tools with deep learning frameworks for image classification.

Comput Biol Med 2019 Apr 6;108:49-56. Epub 2019 Apr 6.

Department of Mathematics and Computer Science of University of La Rioja, Spain. Electronic address:

Background And Objective: Deep learning techniques have been successfully applied to tackle several image classification problems in bioimaging. However, the models created from deep learning frameworks cannot be easily accessed from bioimaging tools such as ImageJ or Icy; this means that life scientists are not able to take advantage of the results obtained with those models from their usual tools. In this paper, we aim to facilitate the interoperability of bioimaging tools with deep learning frameworks. Read More

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http://dx.doi.org/10.1016/j.compbiomed.2019.03.026DOI Listing

Influence of salbutamol on the anticonvulsant potency of the antiepileptic drugs in the maximal electroshock-induced seizures in mice.

Pharmacol Rep 2019 Feb 8;71(3):466-472. Epub 2019 Feb 8.

Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland.

Background: β-Adrenergic receptor agonists are widely used agents in the treatment of asthma or preterm labor. Since prevalence of asthma was shown to be higher in patients with epilepsy and modulation of noradrenergic system activity may modify epilepsy course, the aim of the present study was to examine the effect of salbutamol (SALB), one of the most commonly used β-adrenergic receptor agonist on the anticonvulsant potency of four classical antiepileptic drugs (AEDs): valproate (VPA), carbamazepine (CBZ), phenytoin (DPH) and phenobarbital (PB) in mice subjected to the maximal electroshock (MES)-induced seizures.

Methods: Seizures were caused by a current delivered through ear-clip electrodes. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.02.003DOI Listing
February 2019

Muscarinic activity in hippocampus and entorhinal cortex is crucial for spatial and fear memory retrieval.

Pharmacol Rep 2019 Feb 8;71(3):449-456. Epub 2019 Feb 8.

Neurobiology Laboratory, Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan. Electronic address:

Background: Hippocampus and entorhinal cortex are key players of learning and memory. Despite their established role in memory processes, the contribution of muscarinic receptor activity in these brain regions during memory retrieval remains elusive. This study was aimed to assess the role of hippocampal CA1 and medial entorhinal cortex muscarinic receptors in memory retrieval. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.02.004DOI Listing
February 2019

Methylglyoxal in combination with 5-Fluorouracil elicits improved chemosensitivity in breast cancer through apoptosis and cell cycle inhibition.

Biomed Pharmacother 2019 Apr 16;114:108855. Epub 2019 Apr 16.

Dept. of Biophysics, Bose Institute, P 1/12, CIT Scheme VII M, Kolkata, 700 054, WB, India; Dept. of Chemistry, Institute of Applied Science & Humanities GLA University Mathura, 17km Stone, NH-2, Mathura-Delhi Road, Mathura, 281 406, UP, India. Electronic address:

The anti-carcinogenic effect of Methylglyoxal (MG) is well established. It generally targets malignant cells by affecting glycolysis and mitochondrial respiration with minimum or no toxicity to normal cells. In an initial study we have reported that MG can synergistically act with 5-Fluorouracil (5-FU) to decreases the number of MCF-7 breast cancer cells (Ghosh S, Pal A, Ray M, 2017). Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108855DOI Listing

Habitual and acute exercise effects on salivary biomarkers in response to psychosocial stress.

Psychoneuroendocrinology 2019 Mar 25;106:216-225. Epub 2019 Mar 25.

Department of Sport Science, Albert-Ludwigs-University Freiburg, Schwarzwaldstraße 175, Freiburg 79117, Germany. Electronic address:

Objective: Previous research suggests beneficial effects of physical exercise on stress reactivity due to cross-stressor adaptions of physiological stress response systems. However, results remain inconclusive and it is unclear whether only regular engagement in exercise modulates these physiological adaptations or if acute bouts of exercise can elicit similar adaptations. Thus, the aim of the current study was to investigate and compare the effects of habitual and acute exercise on physiological stress responses. Read More

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http://dx.doi.org/10.1016/j.psyneuen.2019.03.015DOI Listing

Subjective unpleasantness of malodors induces a stress response.

Psychoneuroendocrinology 2019 Mar 22;106:206-215. Epub 2019 Mar 22.

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan; ERATO Touhara Chemosensory Signal Project, JST, The University of Tokyo, Tokyo, 113-8657, Japan; International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study, Tokyo, 113-0033 Japan. Electronic address:

Unpleasant odors impair our mood and may affect physical health, even when the odorants are not toxic. A possible cause for such negative effects is stress induced by odors; however, whether the unpleasantness itself elicited stress or not has not been clear. Thus, we examined whether unpleasantness of odors induced the stress responses of emotion, the hypothalamic-pituitary-adrenal (HPA) axis, and the sympathetic nervous system (SNS). Read More

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http://dx.doi.org/10.1016/j.psyneuen.2019.03.018DOI Listing

Determination of 20 perfluoroalkyl substances in greenhouse vegetables with a modified one-step pretreatment approach coupled with ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS-MS).

Chemosphere 2019 Apr 9;227:470-479. Epub 2019 Apr 9.

College of Food Science and Engineering, Shandong Agricultural University, Tai'an, 271001, PR China. Electronic address:

A new rapid modified one-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) approach was developed for the simultaneous determination of 20 perfluoroalkyl substances (PFASs) from samples of cucumber, lettuce, eggplant, tomato, and leek. Several parameters were optimized. Similar pretreatment approaches were employed for comparison; the results verified the satisfactory performance of this new method. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.034DOI Listing

Calibration of polydimethylsiloxane and polyurethane foam passive air samplers for measuring semi volatile organic compounds using a novel exposure chamber design.

Chemosphere 2019 Apr 8;227:435-443. Epub 2019 Apr 8.

Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail Toronto, Ontario, M1C 1A4, Canada; Department of Earth Sciences, 22 Russell Street, University of Toronto, Toronto, Ontario, M5S 3B1, Canada.

Passive air sampling is increasingly used for air quality monitoring and for personal sampling. In a novel experimental exposure chamber study, 3 types of polydimethylsiloxane (PDMS, including sheet and wristband) and 1 type of polyurethane foam (PUF) passive air samplers were tested for gas-phase uptake of 200 semi volatile organic compounds (SVOCs) during six months. For 155 SVOCs including PAH, PCB, phthalates, organophosphate esters, musk compounds, organochlorine- and other pesticides, a normalized generic uptake rate (Rs) of 7. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.043DOI Listing

Toxic, cytogenetic and antitumor evaluations of [6]-gingerol in non-clinical in vitro studies.

Biomed Pharmacother 2019 Apr 16;115:108873. Epub 2019 Apr 16.

Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Teresina, Piauí, 64049-550, Brazil; Postgraduate Program in Pharmaceutical Sciences, Laboratory of Genetic Toxicology, Federal University of Piauí, Teresina, Piauí, 64049-550, Brazil.

Gingerol - [6]-gingerol ((S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone; [6]-G) - is a phenolic compound with several pharmacological properties. Herein, the aim of the study was to evaluate the toxicogenic effects of [6]-G on Artemia salina nauplii, Allium cepa, HL-60 cell line and Sarcoma 180 (S-180) ascitic fluid cells.For toxic and genotoxic analysis, it was used [6]-G concentrations of 5, 10, 20 and 40 μg mL-1. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108873DOI Listing

Removal of 5-fluorouracil by solar-driven photoelectrocatalytic oxidation using Ti/TiO(NT) photoelectrodes.

Water Res 2019 Apr 9;157:610-620. Epub 2019 Apr 9.

Department of Environmental Technology, Faculty of Chemistry, University of Gdansk, 80-308, Gdansk, Poland. Electronic address:

The efficient and safe degradation of drugs present in wastewater requires the design of a new material possessing high activity for that process. In addition to other methods, photoelectrocatalysis (PEC) merges the strengths of both photocatalytic and electrochemical methods, and the efficiency could be enhanced by the type of photoelectrode material. To address this challenge, three Ti/TiO nanotube-based photoelectrodes, differing in their tube morphology, were prepared by anodic oxidation and employed for the degradation of the 5-fluorouracil (5-FU) drug by the PEC process. Read More

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http://dx.doi.org/10.1016/j.watres.2019.04.010DOI Listing

Masking procedures can influence priming effects besides their effects on conscious perception.

Conscious Cogn 2019 Apr 16;71:92-108. Epub 2019 Apr 16.

Georg-Elias-Müller Institute for Psychology, University of Göttingen, Germany. Electronic address:

Masked priming has been employed to study the role of consciousness for different levels of visual processing. However, masking procedures differ systematically between studies. To examine these procedural differences we contrasted priming effects with metacontrast masking, which is often applied in the context of perceptual priming, and priming effects with sandwich pattern masking, frequently used in studies on semantic priming. Read More

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http://dx.doi.org/10.1016/j.concog.2019.03.009DOI Listing

Shaped pulses for transient compensation in quantum-limited electron spin resonance spectroscopy.

J Magn Reson 2019 Apr 11;303:42-47. Epub 2019 Apr 11.

Quantronics Group, SPEC, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191, Gif-sur-Yvette Cedex, France. Electronic address:

In high sensitivity inductive electron spin resonance spectroscopy, superconducting microwave resonators with large quality factors are employed. While they enhance the sensitivity, they also distort considerably the shape of the applied rectangular microwave control pulses, which limits the degree of control over the spin ensemble. Here, we employ shaped microwave pulses compensating the signal distortion to drive the spins faster than the resonator bandwidth. Read More

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http://dx.doi.org/10.1016/j.jmr.2019.04.008DOI Listing

Associations between urinary organophosphate ester metabolites and measures of adiposity among U.S. children and adults: NHANES 2013-2014.

Environ Int 2019 Apr 16;127:754-763. Epub 2019 Apr 16.

Maryland Institute of Applied Environmental Health, School of Public Health, University of Maryland, College Park, MD, USA. Electronic address:

Background: Organophosphate esters (OPEs) are synthetic chemicals found in many consumer products, including furniture, electronics, processed foods, and building materials. Emerging in vitro and in vivo studies suggest that OPEs are metabolism disrupting compounds; however, epidemiologic studies investigating their associations with adiposity markers are sparse.

Objective: We examined cross-sectional associations between OPE biomarkers and adiposity measures among U. Read More

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http://dx.doi.org/10.1016/j.envint.2019.03.055DOI Listing

Stability of stochastic impulsive reaction-diffusion neural networks with S-type distributed delays and its application to image encryption.

Neural Netw 2019 Apr 4;116:35-45. Epub 2019 Apr 4.

School of Mathematical Sciences, Ocean University of China, Qingdao 266100, China. Electronic address:

In this paper, we study stochastic impulsive reaction-diffusion neural networks with S-type distributed delays, aiming to obtain the sufficient conditions for global exponential stability. First, an impulsive inequality involving infinite delay is introduced and the asymptotic behaviour of its solution is investigated by the truncation method. Then, global exponential stability in the mean-square sense of the stochastic impulsive reaction-diffusion system is studied by constructing a simple Lyapunov-Krasovskii functional where the S-type distributed delay is handled by the impulsive inequality. Read More

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http://dx.doi.org/10.1016/j.neunet.2019.03.016DOI Listing

The origin of hyperpolarization based on the directional conduction of action potential using a model nerve cell system.

Bioelectrochemistry 2019 Apr 4;128:155-164. Epub 2019 Apr 4.

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address:

In nerve cells, changes in local membrane potentials are generated and propagated along a nerve axon mainly by the function of K and Na channels. Generally, concurrent monitoring of multi-points on an axon is performed based on the voltage-clamp method. As the respective membrane potentials have been evaluated by considering the relations between the applied potential, the local current, and conductance, experimental values are not directly evaluated. Read More

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http://dx.doi.org/10.1016/j.bioelechem.2019.03.007DOI Listing

Synthesis, molecular properties and comparative docking and QSAR of new 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetic acid derivatives as possible anticancer agents.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Apr 7;218:248-262. Epub 2019 Apr 7.

Chemistry Department, Faculty of Science, Al-Azhar University (Boys Branch), Nasr City, Cairo, Egypt.

Novel coumarin amino acid derivatives were synthesized. The structure of synthesized compounds has established on basis of different spectral data. The optimization geometry, frontier molecular orbitals (FMOs), thermodynamic parameters and chemical reactivity, were discussed using DFT\B3LYP by 6-311G* basis set, to identify a clear view for inter and intramolecular interaction of tested compounds. Read More

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http://dx.doi.org/10.1016/j.saa.2019.02.074DOI Listing

Synthesis, biological activity and multiscale molecular modeling studies of bis-coumarins as selective carbonic anhydrase IX and XII inhibitors with effective cytotoxicity against hepatocellular carcinoma.

Bioorg Chem 2019 Mar 7;87:838-850. Epub 2019 Mar 7.

Sakarya University of Applied Sciences, Pamukova Vocational Highschool, Pamukova, Turkey.

A series of novel bis-coumarin derivatives containing triazole moiety as a linker between the alkyl chains was synthesized and their inhibitory activity against the human carbonic anhydrase (hCA) isoforms I, II, IX and XII were evaluated. In addition, cytotoxic effects of the synthesized compounds on renal adenocarcinoma (769P), hepatocellular carcinoma (HepG2) and breast adeno carcinoma (MDA-MB-231) cell lines were examined. While the hCA I and II isoforms were inhibited in the micromolar range, the tumor-associated isoform hCA IX and XII were inhibited in the high nanomolar range. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.003DOI Listing

Validation of gamma scanning method for optimizing NaI(Tl) detector model in Monte Carlo simulation.

Appl Radiat Isot 2019 Apr 10;149:1-8. Epub 2019 Apr 10.

Nuclear Technique Laboratory, University of Science, VNU-HCM, Viet Nam; Department of Nuclear Physics, Faculty of Physics and Engineering Physics, University of Science, VNU-HCM, Viet Nam. Electronic address:

The aim of this study is the validation of gamma scanning method for optimizing NaI(Tl) detector model in Monte Carlo simulation. The experimental procedure involved: scanning on front and lateral surfaces of the detector with collimated low-energy photon beam; calibrating the efficiency with energies between 31-1408 keV for point sources at distances of 0 cm and 30 cm from source to the detector. The Monte Carlo code used for the simulations was MCNP6. Read More

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http://dx.doi.org/10.1016/j.apradiso.2019.04.009DOI Listing

Pulmonary nodule detection in CT scans with equivariant CNNs.

Med Image Anal 2019 Mar 28;55:15-26. Epub 2019 Mar 28.

University of Amsterdam, Netherlands.

Convolutional Neural Networks (CNNs) require a large amount of annotated data to learn from, which is often difficult to obtain for medical imaging problems. In this work we show that the sample complexity of CNNs can be significantly improved by using 3D roto-translation group convolutions instead of standard translational convolutions. 3D CNNs with group convolutions (3D G-CNNs) were applied to the problem of false positive reduction for pulmonary nodule detection in CT scans, and proved to be substantially more effective in terms of accuracy, sensitivity to malignant nodules, and speed of convergence compared to a strong and comparable baseline architecture with regular convolutions, extensive data augmentation and a similar number of parameters. Read More

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http://dx.doi.org/10.1016/j.media.2019.03.010DOI Listing

Indigenous Doulas: A literature review exploring their role and practice in western maternity care.

Midwifery 2019 Apr 16;75:52-58. Epub 2019 Apr 16.

Charles Darwin University, Australia.

Objective: The purpose of this article is to establish a body of literature exploring the emergent topic of Indigenous doulas, in relation to Indigenous communities in remote locations, where women are routinely evacuated and no longer supported to give birth. In doing so the article will synthesise and critique key concepts in the literature and identify gaps for prioritisation in future research.

Design: The methodology is influenced by Indigenous, decolonising and feminist theoretical standpoints. Read More

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http://dx.doi.org/10.1016/j.midw.2019.04.005DOI Listing

Safety-Engineered Syringes: An Intervention to Decrease Hepatitis C Burden in Developing Countries-A Cost-Effectiveness Analysis from Egypt.

Value Health Reg Issues 2019 Apr 16;19:51-58. Epub 2019 Apr 16.

HTA Office, L.L.C., Cairo, Egypt; Pharmacy Practice Department, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt. Electronic address:

Introduction: To assess the cost-effectiveness of introducing the safety-engineered syringe (SES) to decrease hepatitis C burden resultant from unsafe injection practices in healthcare settings.

Methods: A Markov process model for a hypothetical study cohort was developed over a 30-year time horizon to compare the adoption of SES use with the current strategy, conventional syringes (CS), in the Egyptian healthcare settings. The national treatment program was applied in both groups. Read More

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http://dx.doi.org/10.1016/j.vhri.2018.11.009DOI Listing

A green deep eutectic solvents microextraction coupled with acid-base induction for extraction of trace phenolic compounds in large volume water samples.

Ecotoxicol Environ Saf 2019 Apr 16;178:130-136. Epub 2019 Apr 16.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province, 650500, China. Electronic address:

A simple, effective and convenient method for determination of phenolic compounds by acid-base induced deep eutectic solvents (DESs) microextraction was developed. The binary and ternary DESs were prepared by a range of fatty acids (C-C), which can act as hydrogen bond donors and hydrogen bond acceptors simultaneously. The gas-assisted mixing customization provides excellent mixing performance and concentration efficiency through the bubble adsorption mechanism for the handling of large-volume aqueous sample. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.021DOI Listing

Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: Characterization of four fatty acyl elongases and a novel desaturase capacity.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education of China, Ningbo, Zhejiang 315211, China. Electronic address:

As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.04.004DOI Listing

A machine learning approach to knee osteoarthritis phenotyping: Data from the FNIH Biomarkers Consortium.

Osteoarthritis Cartilage 2019 Apr 16. Epub 2019 Apr 16.

Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address:

Objective: Knee osteoarthritis (KOA) is a heterogeneous condition representing a variety of potentially distinct phenotypes. The purpose of this study was to apply innovative machine learning approaches to KOA phenotyping in order to define progression phenotypes that are potentially more responsive to interventions.

Design: We used publicly available data from the FNIH OA Biomarkers Consortium, where radiographic (medial joint space narrowing of ≥0. Read More

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http://dx.doi.org/10.1016/j.joca.2018.12.027DOI Listing

(-)-α-Bisabolol reduces nociception and trigeminal central sensitisation in acute orofacial neuropathic pain induced by infraorbital nerve injury.

Life Sci 2019 Apr 16. Epub 2019 Apr 16.

Faculty of Dentistry, University of Toronto, Canada; Deparment of Physiology, University of Toronto, Canada.

Neuropathic orofacial pain conditions represent a challenge to diagnose and treat. Natural substances are promising therapeutic options for the control of pain.

Aims: This study aimed to examine whether (-)-α-bisabolol (BISA), a natural terpene, can attenuate nociceptive behaviour and central sensitisation in a rodent model of trigeminal neuropathic pain. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.04.032DOI Listing

Nanocrystalline cellulose: Preparation, physicochemical properties, and applications in drug delivery systems.

Int J Biol Macromol 2019 Apr 16. Epub 2019 Apr 16.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Cancer is the leading cause of death all over the world and chemotherapy is an important approach to fight cancer, however, there are many obstacles against successful cancer chemotherapy such as development of multidrug resistance, poor solubility of chemotherapeutic agents and adverse side effects to healthy tissues. An important strategy to overcome these obstacles, is the use of nanotechnology. In recent years, natural polymers such as cellulose and its nanoform structure, nanocrystalline cellulose (NCC), have attracted the interest of researchers in the field of nanotechnology and specially drug delivery systems, due to biocompatibility and biodegradability of NCC. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.117DOI Listing

Competencies on environmental health and pedagogical approaches in the nursing curriculum: A systematic review of the literature.

Nurse Educ Pract 2019 Apr 5;37:1-8. Epub 2019 Apr 5.

Health Service Research, Faculty of Health and Human Sciences, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address:

It has been suggested that climate change is the biggest threat to public health for the 21st Century; increased demand on health services will impact on already overstretched resources and systems will need to be able to respond. However limited attention is given to climate change and sustainability in nursing education; there is no clear guidance on curricula content for nurses or recommendations regarding the skills and competencies that will be required. Literature published in Dutch, English, German, and Spanish was searched and 32 papers met the inclusion criteria for the review. Read More

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http://dx.doi.org/10.1016/j.nepr.2019.04.004DOI Listing

In- vitro-activity of additive application of hydrogen peroxide in antimicrobial photodynamic therapy using LED in the blue spectrum against bacteria and biofilm being associated with periodontal disease.

Photodiagnosis Photodyn Ther 2019 Apr 16. Epub 2019 Apr 16.

School of Dentistry, Department of Periodontology, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. Electronic address:

Background: Although antimicrobial photodynamic therapy (aPDT) has been shown to be efficient in killing planktonic periodontopathogenic bacteria, its activity on established biofilms is very limited. The aim of the present in-vitro study was to evaluate the potential effect of hydrogen peroxide as a pretreatment for aPDT.

Methods: aPDT consisting of riboflavin as photosensitizer and illumination by a LED lamp emitting in the blue spectrum for 30 s and 60 s (aPDT60) was combined with a pretreatment with 0. Read More

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http://dx.doi.org/10.1016/j.pdpdt.2019.04.015DOI Listing

Precise editing of plant genomes - prospects and challenges.

Semin Cell Dev Biol 2019 Apr 16. Epub 2019 Apr 16.

Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. Electronic address:

The past decade has witnessed unprecedented development in genome engineering, a process that enables targeted modification of genomes. The identification of sequence-specific nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas system, in particular, has led to precise and efficient introduction of genetic variations into genomes of various organisms. Since the CRISPR/Cas system is highly versatile, cost-effective and much superior to ZFNs and TALENs, its widespread adoption by the research community has been inevitable. Read More

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http://dx.doi.org/10.1016/j.semcdb.2019.04.010DOI Listing

Infrared spectroscopic study of the coil-helix transition of highly concentrated gelatin formulations.

Eur J Pharm Biopharm 2019 Apr 16. Epub 2019 Apr 16.

University of Heidelberg, IPMB, Department of Pharmaceutical Technology and Biopharmaceutics, Im Neuenheimer Feld 329, 69120 Heidelberg, Germany. Electronic address:

The aim of this study was to investigate the applicability of ATR-FTIR spectroscopy as an analytical tool to monitor the gel formation of highly concentrated gelatin formulations. Spectral changes induced by the coil-helix transition have been studied and related to the elasticity parameter G' obtained by oscillatory rheology in simultaneous measurements. A principal component analysis of the amide I band allowed the evaluation of triple helix formation kinetics. Read More

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http://dx.doi.org/10.1016/j.ejpb.2019.04.010DOI Listing

Corrigendum to Stage-specific metabolic features of differentiating neurons: Implications for toxicant sensitivity Toxicol Appl Pharmacol, 354 (2018) 64-80.

Toxicol Appl Pharmacol 2019 Apr 16. Epub 2019 Apr 16.

In Vitro Toxicology and Biomedicine, Dept Inaugurated by the Doerenkamp-Zbinden Foundation, University of Konstanz, 78457 Konstanz, Germany; CAAT-Europe, University of Konstanz, Konstanz 78457, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.taap.2019.04.010DOI Listing

The RAG2 gene of yellow catfish (Tachysurus fulvidraco) and its immune response against Edwardsiella ictaluri infection.

Dev Comp Immunol 2019 Apr 16. Epub 2019 Apr 16.

Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China. Electronic address:

Recombination-activating gene 2 (rag 2) allies with recombination-activating gene 1 (rag 1) and regulates the V(D)J recombination of immunoglobulin (Ig) and T-cell receptor (TCR) genes. Being a key player in the adaptive immune response of vertebrates, functional characterization of rag 2 from yellow catfish is beneficial for understanding the biological response towards the pathogens. In this report, we have cloned and characterized the rag 2 gene of yellow catfish, and a particular pattern of expression was analysed in the major tissues of yellow catfish. Read More

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http://dx.doi.org/10.1016/j.dci.2019.04.006DOI Listing

Molecular identification of GnIH and its potential role in reproductive physiology and male pregnancy of the lined seahorse (Hippocampus erectus).

Gen Comp Endocrinol 2019 Apr 16. Epub 2019 Apr 16.

CAS Key Laboratory ofTropical Marine Bio-resourcesand Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Institute of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

The gonadotropin-inhibitory hormone (GnIH) plays a negative role in the hypothalamic-pituitary-gonadal (HPG) axis by inhibiting gonadotropin secretion in vertebrates. Male pregnancy and ovoviviparous behavior are unique phenomena among vertebrates. To better understand the neuroendocrine regulatory mechanisms in ovoviviparous fish with male pregnancy, we identified the orthologous GnIH gene in the lined seahorse (Hippocampus erectus). Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.04.018DOI Listing

Vacuum Induced Surface Freezing for the Freeze-Drying of the Human Growth Hormone: How does Nucleation Control Affect Protein Stability?

J Pharm Sci 2019 Apr 16. Epub 2019 Apr 16.

Standardisation Science, National Institute for Biological Standards and Control, Blanche Lane, Medicines & Healthcare products Agency, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK. Electronic address:

In the present work, the effect of controlled nucleation on the stability of human growth hormone (hGH) during freeze-drying has been investigated. More specifically, the vacuum induced surface freezing technique has been compared to conventional freezing, both with and without an annealing step. Size exclusion chromatography and cell-based potency assays have been used to characterize the formation of soluble aggregates and the biological activity of hGH, respectively. Read More

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http://dx.doi.org/10.1016/j.xphs.2019.04.014DOI Listing

Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune competence.

J Med Virol 2019 Apr 19. Epub 2019 Apr 19.

Department of Translational Research, University of Pisa, Pisa, Italy.

Background: Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict post-transplant immune-related complications, and eventually to customize immunosuppression.

Methods: In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE® assay. Read More

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http://dx.doi.org/10.1002/jmv.25488DOI Listing

Single-cell correlations of mRNA and protein content in a human monocytic cell line after LPS stimulation.

PLoS One 2019 19;14(4):e0215602. Epub 2019 Apr 19.

Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America.

The heterogeneity of mRNA and protein expression at the single-cell level can reveal fundamental information about cellular response to external stimuli, including the sensitivity, timing, and regulatory interactions of genes. Here we describe a fully automated system to digitally count the intron, mRNA, and protein content of up to five genes of interest simultaneously in single-cells. Full system automation of 3D microscope scans and custom image analysis routines allows hundreds of individual cells to be automatically segmented and the mRNA-protein content to be digitally counted. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215602PLOS

Markerless motion tracking and correction for PET, MRI, and simultaneous PET/MRI.

PLoS One 2019 19;14(4):e0215524. Epub 2019 Apr 19.

Department of Applied Mathematics and Computer Science, Technical University of Denmark, Lyngby, Denmark.

Objective: We demonstrate and evaluate the first markerless motion tracker compatible with PET, MRI, and simultaneous PET/MRI systems for motion correction (MC) of brain imaging.

Methods: PET and MRI compatibility is achieved by careful positioning of in-bore vision extenders and by placing all electronic components out-of-bore. The motion tracker is demonstrated in a clinical setup during a pediatric PET/MRI study including 94 pediatric patient scans. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215524PLOS

Patterns of fish utilisation in a tropical Indo-Pacific mangrove-coral seascape, New Caledonia.

PLoS One 2019 19;14(4):e0207168. Epub 2019 Apr 19.

College of Science and Engineering, James Cook University, Townsville, Queensland, Australia.

Mangrove forests are important habitats for fish. However, their utilisation by fish, and the specific values they confer, are still not fully understood. This study describes how fish use mangrove forests in an Indo-Pacific mangrove-coral reef seascape. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207168PLOS

Evaluating and using existing models to map probable suitable habitat for rare plants to inform management of multiple-use public lands in the California desert.

PLoS One 2019 19;14(4):e0214099. Epub 2019 Apr 19.

California State Office, Bureau of Land Management, Sacramento, California, United States of America.

Multiple-use public lands require balancing diverse resource uses and values across landscapes. In the California desert, there is strong interest in renewable energy development and important conservation concerns. The Bureau of Land Management recently completed a land-use plan for the area that provides protection for modeled suitable habitat for multiple rare plants. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214099PLOS

Coupling Langmuir with Michaelis-Menten-A practical alternative to estimate Se content in rice?

PLoS One 2019 19;14(4):e0214219. Epub 2019 Apr 19.

Technical University of Berlin (TUB), Institute of Applied Geosciences, Berlin, Germany.

Selenium plays an important, but vastly neglected role in human nutrition with a narrow gap between dietary deficiency and toxicity. For a potential biofortification of food with Se, as well as for toxicity-risk assessment in sites contaminated by Se, modelling of local and global Se cycling is essential. As bioavailability of Se for rice plants depends on the speciation of Se and the resulting interactions with mineral surfaces as well as the interaction with Se uptake mechanisms in plants, resulting plant Se content is complex to model. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214219PLOS

In vitro chondroprotective potential of Senna alata and Senna tora in porcine cartilage explants and their species differentiation by DNA barcoding-high resolution melting (Bar-HRM) analysis.

PLoS One 2019 19;14(4):e0215664. Epub 2019 Apr 19.

Excellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, Thailand.

Senna species and anthraquinone derivatives generated by these organisms, rhein and aloe-emodin, exert anti-inflammatory effects. These species present a similar morphology but produce different ingredients when they are used as medicinal products. In this study, a DNA barcoding- (Bar-) high-resolution melting (HRM) technique was developed using internal transcribed sequence 2 (ITS2) to differentiate between Senna alata and Senna tora as a result of significant differences in their melting profiles. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215664PLOS

Phase transitions of the typical algorithmic complexity of the random satisfiability problem studied with linear programming.

PLoS One 2019 19;14(4):e0215309. Epub 2019 Apr 19.

Institut für Physik, Universität Oldenburg, Oldenburg, Germany.

Here we study linear programming applied to the random K-SAT problem, a fundamental problem in computational complexity. The K-SAT problem is to decide whether a Boolean formula with N variables and structured as a conjunction of M clauses, each being a disjunction of K variables or their negations is satisfiable or not. The ensemble of random K-SAT attracted considerable interest from physicists because for a specific ratio αs = M/N it undergoes in the limit of large N a sharp phase transition from a satisfiable to an unsatisfiable phase. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215309PLOS

CancerInSilico: An R/Bioconductor package for combining mathematical and statistical modeling to simulate time course bulk and single cell gene expression data in cancer.

PLoS Comput Biol 2019 Apr 19;14(4):e1006935. Epub 2019 Apr 19.

Department of Oncology, Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD United States of America.

Bioinformatics techniques to analyze time course bulk and single cell omics data are advancing. The absence of a known ground truth of the dynamics of molecular changes challenges benchmarking their performance on real data. Realistic simulated time-course datasets are essential to assess the performance of time course bioinformatics algorithms. Read More

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http://dx.doi.org/10.1371/journal.pcbi.1006935DOI Listing

Survival of Mycobacterium bovis BCG oral vaccine during transit through a dynamic in vitro model simulating the upper gastrointestinal tract of badgers.

PLoS One 2019 19;14(4):e0214859. Epub 2019 Apr 19.

Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, United Kingdom.

In developing an oral bait BCG vaccine against tuberculosis in badgers we wanted to understand the conditions of the gastrointestinal tract and their impact on vaccine viability. Conditions mimicking stomach and small-intestine caused substantial reduction in BCG viability. We performed in vivo experiments using a telemetric pH monitoring system and used the data to parameterise a dynamic in vitro system (TIM-1) of the stomach and small intestine. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214859PLOS

A fast two-stage active contour model for intensity inhomogeneous image segmentation.

PLoS One 2019 19;14(4):e0214851. Epub 2019 Apr 19.

Department of Applied Mathematics, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.

This paper presents a fast two-stage image segmentation method for intensity inhomogeneous image using an energy function based on a local region-based active contour model with exponential family. In the first stage, we preliminary segment the down-sampled images by the local correntropy-based K-means clustering model with exponential family, which can fast obtain a coarse result with low computational complexity. Subsequently, by taking the up-sampled contour of the first stage as initialization, we precisely segment the original images by the improved local correntropy-based K-means clustering model with exponential family in the second stage. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214851PLOS

Investigating the deregulation of metabolic tasks via Minimum Network Enrichment Analysis (MiNEA) as applied to nonalcoholic fatty liver disease using mouse and human omics data.

PLoS Comput Biol 2019 Apr 19;15(4):e1006760. Epub 2019 Apr 19.

Laboratory of Computational Systems Biotechnology, EPFL, Lausanne, Switzerland.

Nonalcoholic fatty liver disease (NAFLD) is associated with metabolic syndromes spanning a wide spectrum of diseases, from simple steatosis to the more complex nonalcoholic steatohepatitis. To identify the deregulation that occurs in metabolic processes at the molecular level that give rise to these various NAFLD phenotypes, algorithms such as pathway enrichment analysis (PEA) can be used. These analyses require the use of predefined pathway maps, which are composed of reactions describing metabolic processes/subsystems. Read More

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http://dx.doi.org/10.1371/journal.pcbi.1006760DOI Listing
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