115,936 results match your criteria Applicable Analysis And Discrete Mathematics[Journal]


Adaptation and Validation of the Health Belief Model Scale for Breast Self-Examination in Mexican Women.

Value Health Reg Issues 2020 May 31;23:30-36. Epub 2020 May 31.

Psychology School, Center for Research and Development in Health Sciences, Autonomous University of Nuevo Leon, Monterrey, Mexico.

Objectives: In Mexico, breast cancer is often not detected until it is already at an advanced stage. Many women know about breast self-examination (BSE), but few do it correctly. BSE can assist in the early detection of breast cancer, and a valid and reliable instrument to help determine the factors of the Health Belief Model that affect the practice of BSE in Mexican women would thus be advantageous. Read More

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http://dx.doi.org/10.1016/j.vhri.2019.11.006DOI Listing

Biomarker responses in terrestrial gastropods exposed to pollutants: A comprehensive review.

Chemosphere 2020 May 27;257:127218. Epub 2020 May 27.

Department of Animal Pests, Plant Protection Research Institute, Agricultural Research Center, Alexandria, Egypt.

The chemical contamination of terrestrial ecosystems is a great concern as these ecosystems are the target of most of the pollutants derived from anthropogenic activities such as pesticides, heavy metals, nanoparticles, and others. Terrestrial gastropods are considered to be excellent sentinel organisms for biological monitoring of environmental pollution, as they have the ability to accumulate chemicals in their tissues and exhibit a great potential to evaluate the ecological effects of pollutants in terrestrial ecosystems. The use of biomarkers as sensitive parameters to estimate the exposure or resulting effects of chemicals have received considerable attention. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2020.127218DOI Listing

Incorporating discrete choice experiments into policy decisions: Case of designing public long-term care insurance.

Soc Sci Med 2020 May 20;258:113044. Epub 2020 May 20.

Puey Ungphakorn Institute for Economic Research, Bank of Thailand, Thailand. Electronic address:

Discrete choice experiments (DCEs) have been widely used to elicit preferences in the health economics field but recent reviews found that DCE results are rarely incorporated into health policy decisions. We conjecture that one reason is most health policy practitioners only focus on estimating marginal willingness to pay, the measure that is not directly applicable for policy-related questions. We show that when designing a new program, translating preference information into the demand for packages and benefits of alternative schemes (the choices made available) can make the DCE results more policy relevant. Read More

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http://dx.doi.org/10.1016/j.socscimed.2020.113044DOI Listing

Determinants of Recurrent Falls After Stroke: A One-Year Follow-Up of The Fall Study of Gothenburg.

Arch Phys Med Rehabil 2020 Jun 1. Epub 2020 Jun 1.

Region Västra Götaland, Department of Physiotherapy, Sahlgrenska University Hospital/Östra, Gothenburg; Department of Rehabilitation, Clinical Science, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg.

Objective: To identify the occurrence of recurrent falls and the determinants in the acute phase post stroke that are associated with recurrent falls within the first year after stroke onset.

Design: Prospective follow-up study.

Setting: Stroke unit and community. Read More

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http://dx.doi.org/10.1016/j.apmr.2020.05.010DOI Listing

Phenotypic characteristics of patients with Chronic Obstructive Pulmonary Disease after stratification for the Short Physical Performance Battery summary score.

Arch Phys Med Rehabil 2020 Jun 1. Epub 2020 Jun 1.

Department of Research and Development, CIRO, Horn, the Netherlands; Reval Rehabilitation Research - Biomedical Research Institute, Faculty of Rehabilitation Sciences, Hasselt University, Diepenbeek, Belgium; Department of Respiratory Medicine, Maastricht University Medical Centre (MUMC+), NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht, The Netherlands.

Objective: To assess the phenotypic characteristics of patients with Chronic Obstructive Pulmonary Disease (COPD) after stratification for Short Physical Performance Battery (SPPB) summary scores and to determine phenotypic characteristics of the SPPB summary score at the start of pulmonary rehabilitation (PR).

Design: Retrospective, cross-sectional.

Setting: Baseline assessment for PR program. Read More

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http://dx.doi.org/10.1016/j.apmr.2020.05.011DOI Listing

The SMAC mimetic LCL161 is a direct ABCB1/MDR1-ATPase activity modulator and BIRC5/Survivin expression down-regulator in cancer cells.

Toxicol Appl Pharmacol 2020 Jun 1:115080. Epub 2020 Jun 1.

Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address:

Upregulation of ABCB1/MDR1 (P-gp) and BIRC5/Survivin promotes multidrug resistance in a variety of human cancers. LCL161 is an anti-cancer DIABLO/SMAC mimetic currently being tested in patients with solid tumors, but the molecular mechanism of action of LCL161 in cancer cells is still incompletely understood. It is still unclear whether LCL161 is therapeutically applicable for patients with ABCB1-overexpressing multidrug resistant tumors. Read More

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http://dx.doi.org/10.1016/j.taap.2020.115080DOI Listing

Lentiviral-Vector-Based Dendritic Cell Vaccine Synergizes with Checkpoint Blockade to Clear Chronic Viral Infection.

Mol Ther 2020 May 20. Epub 2020 May 20.

Department of Microbiology, New York University Langone Medical Center, New York, NY 10016, USA. Electronic address:

Dendritic cell vaccines are a promising strategy for the treatment of cancer and infectious diseases but have met with mixed success. We report on a lentiviral vector-based dendritic cell vaccine strategy that generates a cluster of differentiation 8 (CD8) T cell response that is much stronger than that achieved by standard peptide-pulsing approaches. The strategy was tested in the mouse lymphocytic choriomeningitis virus (LCMV) model. Read More

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http://dx.doi.org/10.1016/j.ymthe.2020.05.018DOI Listing

High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-Down.

Mol Cell 2020 May 29. Epub 2020 May 29.

Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

The identification of microRNA (miRNA) targets by Ago2 crosslinking-immunoprecipitation (CLIP) methods has provided major insights into the biology of this important class of non-coding RNAs. However, these methods are technically challenging and not easily applicable to an in vivo setting. To overcome these limitations and facilitate the investigation of miRNA functions in vivo, we have developed a method based on a genetically engineered mouse harboring a conditional Halo-Ago2 allele expressed from the endogenous Ago2 locus. Read More

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http://dx.doi.org/10.1016/j.molcel.2020.05.009DOI Listing

Design and High-Resolution Characterization of Silicon Wafer-like Omniphobic Liquid Layers Applicable to Any Substrate.

ACS Appl Mater Interfaces 2020 Jun 4. Epub 2020 Jun 4.

Liquid fouling can reduce the functionality of critical engineering surfaces. Recent studies have shown that minimizing contact angle hysteresis is a promising strategy for achieving omniphobic (all-liquid repellent) properties, thereby inhibiting fouling. Prior omniphobic films can repel a broad range of liquids, but the applicability of these coatings has always been limited to silicon wafers or smooth glass. Read More

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http://dx.doi.org/10.1021/acsami.0c06433DOI Listing

Critical appraisal of the International Prophylaxis Study Group magnetic resonance image scale for evaluating haemophilic arthropathy.

Haemophilia 2020 Jun 4. Epub 2020 Jun 4.

Department of Diagnostic Imaging, The Hospital for Sick Children, University of Toronto, Toronto, Canada.

A goal of the International Prophylaxis Study Group (IPSG) is to provide an accurate instrument to measure MRI-based disease severity of haemophilic arthropathy at various time points, so that longitudinal changes in disease severity can be identified to support decisions on treatment management. We review and discuss in this paper the evaluative purpose of the IPSG MRI scale in relation to its development and validation processes so far. We also critically appraise the validity, reliability and responsiveness of using the IPSG MRI scale in different clinical and research settings, and whenever applicable, compare these clinimetric properties of the IPSG MRI scale with those of its precursors, the compatible additive and progressive MRI scales. Read More

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http://dx.doi.org/10.1111/hae.14032DOI Listing

Patients with hip resurfacing arthroplasty are not physically more active than those with a stemmed total hip.

Acta Orthop 2020 Jun 4:1-5. Epub 2020 Jun 4.

Luxembourg Institute of Health, Human Motion, Orthopedics, Sports Medicine, Digital Methods (HOSD), Luxembourg, Luxembourg.

Background and purpose - Hip resurfacing arthroplasty (HRA) was designed for the highly active patient because of the various theoretical advantages compared with stemmed total hip arthroplasty (THA), but has shown high failure rates. Physical activity (PA) after arthroplasty is frequently determined with the use of questionnaires, which are known for their subjective nature, recall bias, and ceiling effect. These disadvantages are not applicable to physical activity monitoring (AM) using sensors. Read More

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http://dx.doi.org/10.1080/17453674.2020.1771652DOI Listing

Solvent-Dependent Mechanism and Stereochemistry of Mitsunobu Glycosylation with Unprotected Pyranoses.

Org Lett 2020 Jun 4. Epub 2020 Jun 4.

Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.

An S2 mechanism was proposed for highly stereoselective glycosylation of benzoic acid with unprotected α-d-glucose under Mitsunobu conditions in dioxane, while an S1 mechanism was indicated for nonstereoselective glycosylation in DMF. The S2-type stereoselective Mitsunobu glycosylation is generally applicable to various unprotected pyranoses as glycosyl donors in combination with a wide range of acidic glycosyl acceptors such as carboxylic acids, phenols, and imides, retaining its high stereoselectivity (33 examples). Glycosylation of a carboxylic acid with unprotected α-d-mannose proceeded also in an S2 manner to directly afford a usually less accessible 1,2--mannoside. Read More

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http://dx.doi.org/10.1021/acs.orglett.0c01549DOI Listing

High-precision Atomic Charge Prediction for Protein Systems Using Fragment Molecular Orbital Calculation and Machine Learning.

J Chem Inf Model 2020 Jun 4. Epub 2020 Jun 4.

Here, we have constructed neural network-based models that predict atomic partial charges with high accuracy at low computational cost. The models were trained by high-quality data acquired by quantum mechanics calculations using the fragment molecular orbital method. We have succeeded in obtaining highly accurate atomic partial charges for three representative molecular systems of proteins, including one large biomolecule (approx. Read More

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http://dx.doi.org/10.1021/acs.jcim.0c00273DOI Listing

Sensitive Untargeted Screening of Nerve Agents and Their Degradation Products Using Liquid Chromatography-High Resolution Mass Spectrometry.

Anal Chem 2020 Jun 4. Epub 2020 Jun 4.

Nerve agents (NAs) are notorious chemical warfare agents that pose a serious threat to national security and public health. The total number of theoretical chemicals of NAs and their degradation products (DPs) exceeds 410,000, according to 1.A. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01508DOI Listing

A multispecies assessment of wildlife impacts on local community livelihoods.

Conserv Biol 2020 Jun 4. Epub 2020 Jun 4.

Biological and Environmental Sciences, University of Stirling, Stirling, FK9 4LA, UK.

Conflicts between the interests of agriculture and wildlife conservation are a major threat to biodiversity and human wellbeing globally. Addressing such conflicts requires a thorough understanding of the impacts associated with living alongside protected wildlife. Despite this, most studies reporting on human-wildlife impacts and the strategies used to mitigate them focus on a single species, thus over-simplifying often complex systems of human-wildlife interactions. Read More

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http://dx.doi.org/10.1111/cobi.13565DOI Listing

Adaptation and Acceptability of a Digitally Delivered Intervention for Parents of Very Low Birth Weight Infants.

Nurs Res 2020 Jun 1. Epub 2020 Jun 1.

Department of Pediatrics, Rush University Medical Center, Chicago, IL Department of Pediatrics, Rush University Medical Center, Chicago, IL Rush University Medical Center, Chicago, IL Rush University Medical Center, Chicago, IL Rush University Medical Center, Chicago, IL.

Background: Very low birth weight infants (VLBW) are at elevated risk for behavioral problems as early as the second year of life. The purpose of this feasibility study was to evaluate the adaptation and acceptability of an existing digitally delivered behavioral parent training (BPT) program-the ezParent program, with the addition of weekly coaching calls-for parents of former VLBW infants in their second year of life.

Objectives: To assess the adaptation of ezParent for this population, we assessed parent access and use of ezParent and coaching calls; parent learning of strategies from ezParent; and changes in parenting practices or child behavior after using ezParent plus coaching calls. Read More

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http://dx.doi.org/10.1097/NNR.0000000000000445DOI Listing

Non-Uniform Sampling in NMR Spectroscopy and the Preservation of Spectral Knowledge in the Time and Frequency Domains.

J Phys Chem A 2020 Jun 4. Epub 2020 Jun 4.

The increased sensitivity under weighted non-uniform sampling (NUS) is demonstrated and quantified using Monte Carlo simulations of nuclear magnetic resonance time and frequency-domain signals. The concept of spectral knowledge is introduced and shown to be superior to the frequency-domain signal-to-noise ratio for assessing the quality of NMR data. Two methods for rigorously preserving spectral knowledge and the time-domain NUS knowledge enhancement upon transformation to the frequency domain are demonstrated, both theoretically and numerically. Read More

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http://dx.doi.org/10.1021/acs.jpca.0c02930DOI Listing

A simple method for production of hydrophilic, rigid, and sterilized multi-layer 3D integrated polydimethylsiloxane microfluidic chips.

Lab Chip 2020 Jun 4. Epub 2020 Jun 4.

Quantum Beam Science Research Directorate, National Institutes for Quantum and Radiological Science and Technology (QST), 1233 Watanuki-machi, Takasaki-shi, Gunma 370-1292, Japan.

Polydimethylsiloxane (PDMS) has many desirable features for microfluidics applications, particularly in diagnostics and pharmaceuticals, but its hydrophobicity and the lack of a practical method for bonding PDMS layers limit its use. Moreover, the flexibility of PDMS causes unwanted deformation during use in some applications. Here, we report a simple method for solving these problems simultaneously using an electron beam (EB) or γ-rays, which are commonly used for sterilizing medical products. Read More

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http://dx.doi.org/10.1039/d0lc00316fDOI Listing

[Biological characteristics of wild Angelica sinensis seed].

Zhongguo Zhong Yao Za Zhi 2020 May;45(10):2368-2373

Tianjin University of Traditional Chinese Medicine Tianjin 301617, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

Wild Angelica sinensis is almost endangered, studying the biological characteristics of wild A. sinensis seeds is helpful for varietal improvement and its conservation. This paper systematically studied the morphological structure, thousand seed weight, viability, storage and other basic biological characteristics of wild A. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200302.114DOI Listing

[Expert consensus on Shujin Jianyao Pills in clinical practice].

Zhongguo Zhong Yao Za Zhi 2020 May;45(10):2300-2303

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China.

Shujin Jianyao Pills is a Chinese patent medicine, with anti-inflammation, analgesic and anti-osteoporotic effects. Based on a questionnaire investigation of clinicians and a systematic review of study literatures on Shujin Jianyao Pills, the international clinical practice guidelines development method was adopted to analyze the optimal available evidences and expert experiences in the "evidence-based, consensus-based and experience-based" principles. Expert consensus on Shujin Jianyao Pills in clinical practice(GS/CACM267-2019) was developed by more than 30 multidisciplinary experts nationwide with the aim to guide and standardize the rational use of Shujin Jianyao Pills among clinicians and improve the clinical efficacy and safety. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200218.501DOI Listing

Pelvic lymph node distribution and metastases of prostate and bladder cancer: a systematic literature review and template proposal.

World J Urol 2020 Jun 3. Epub 2020 Jun 3.

Department of Urology, The Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX, Amsterdam, Netherlands.

Purpose: To systematically review the relevant literature that evaluates the LN topographical distribution and propose a uniform template.

Methods: A bibliographic search of PubMed/Medline, Embase and SCOPUS was performed for studies reporting data of LN imaging and/or nodal resection.

Results: 101 and 26 articles met the inclusion criteria for PCa and BCa, respectively. Read More

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http://dx.doi.org/10.1007/s00345-020-03281-1DOI Listing

Towards screening Barrett's oesophagus: current guidelines, imaging modalities and future developments.

Clin J Gastroenterol 2020 Jun 3. Epub 2020 Jun 3.

Biocel Ltd, Hull, HU10 7TS, UK.

Barrett's oesophagus is the only known precursor to oesophageal adenocarcinoma (OAC). Although guidelines on the screening and surveillance exist in Barrett's oesophagus, the current strategies are inadequate. Oesophagogastroduodenoscopy (OGD) is the gold standard method in screening for Barrett's oesophagus. Read More

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http://dx.doi.org/10.1007/s12328-020-01135-2DOI Listing

The Impact of Racially Motivated Housing Discrimination on Allostatic Load among Indigenous University Students.

J Urban Health 2020 Jun 3. Epub 2020 Jun 3.

Department of Kinesiology and Physical Education, Faculty of Arts & Science, University of Lethbridge, 4401 University Drive, Lethbridge, AB, T1K3M4, Canada.

Allostatic load (AL) is an aggregate measure of wear and tear on the body due to the chronic activation of the stress response system. The goal of this study was to examine the association between racially motivated housing discrimination (HD) and AL score within a sample of Indigenous university students. Data for this cross-sectional study were collected from Indigenous adults attending university in a small city in western Canada between 2015 and 2017 (N = 104; mean age = 27. Read More

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http://dx.doi.org/10.1007/s11524-020-00446-6DOI Listing

Establishment of reference intervals of biochemical analytes for healthy Chinese volunteers during the screening process in clinical pharmacology trials.

Eur J Clin Pharmacol 2020 Jun 3. Epub 2020 Jun 3.

BE and Phase I Clinical Trial Center, The First Affiliated Hospital of Xiamen University, NO.11 Hongdi Road, Xiamen, Fujian, 361022, People's Republic of China.

Purpose: Laboratory reference intervals (RIs) play a key role in clinical pharmacology trials, both in the screening process and in evaluating drug safety. However, RIs tend to be confined to the general population, and data about RIs for the trial population are limited. The purpose of this study was to determine appropriate RIs for use when screening a defined special subgroup of a healthy Chinese population in clinical pharmacology trials. Read More

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http://dx.doi.org/10.1007/s00228-020-02912-1DOI Listing

The development of a new accountability measurement framework and tool for global health initiatives.

Health Policy Plan 2020 Jun 3. Epub 2020 Jun 3.

Options Consultancy Services Ltd, St Magnus House, 3 Lower Thames Street, London EC3R 6HD, UK.

The Global Strategy for Women's Children's and Adolescents' Health emphasizes accountability as essential to ensure that decision-makers have the information required to meet the health needs of their populations and stresses the importance of tracking resources, results, and rights to see 'what works, what needs improvement and what requires increased attention'. However, results from accountability initiatives are mixed and there is a lack of broadly applicable, validated tools for planning, monitoring and evaluating accountability interventions. This article documents an effort to transform accountability markers-including political will, leadership and the monitor-review-act cycle-into a measurement tool that can be used prospectively or retrospectively to plan, monitor and evaluate accountability initiatives. Read More

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http://dx.doi.org/10.1093/heapol/czz170DOI Listing

Observation of generalized Kibble-Zurek mechanism across a first-order quantum phase transition in a spinor condensate.

Sci Adv 2020 May 22;6(21):eaba7292. Epub 2020 May 22.

Center for Quantum Information, IIIS, Tsinghua University, Beijing 100084, P.R. China.

The Kibble-Zurek mechanism provides a unified theory to describe the universal scaling laws in the dynamics when a system is driven through a second-order quantum phase transition. However, for first-order quantum phase transitions, the Kibble-Zurek mechanism is usually not applicable. Here, we experimentally demonstrate and theoretically analyze a power-law scaling in the dynamics of a spin-1 condensate across a first-order quantum phase transition when a system is slowly driven from a polar phase to an antiferromagnetic phase. Read More

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http://dx.doi.org/10.1126/sciadv.aba7292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244309PMC

Nanofibrillar networks enable universal assembly of superstructured particle constructs.

Sci Adv 2020 May 8;6(19):eaaz7328. Epub 2020 May 8.

Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076 Espoo, Finland.

Superstructured colloidal materials exploit the synergies between components to develop new or enhanced functions. Cohesion is a primary requirement for scaling up these assemblies into bulk materials, and it has only been fulfilled in case-specific bases. Here, we demonstrate that the topology of nanonetworks formed from cellulose nanofibrils (CNFs) enables robust superstructuring with virtually any particle. Read More

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http://dx.doi.org/10.1126/sciadv.aaz7328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209999PMC

Leveraging elastic instabilities for amplified performance: Spine-inspired high-speed and high-force soft robots.

Sci Adv 2020 May 8;6(19):eaaz6912. Epub 2020 May 8.

Department of Mechanical Engineering, Temple University, 1947 North 12th Street, Philadelphia, PA 19122, USA.

Soft machines typically exhibit slow locomotion speed and low manipulation strength because of intrinsic limitations of soft materials. Here, we present a generic design principle that harnesses mechanical instability for a variety of spine-inspired fast and strong soft machines. Unlike most current soft robots that are designed as inherently and unimodally stable, our design leverages tunable snap-through bistability to fully explore the ability of soft robots to rapidly store and release energy within tens of milliseconds. Read More

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http://dx.doi.org/10.1126/sciadv.aaz6912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209986PMC

Targeting therapeutic vulnerabilities with PARP inhibition and radiation in IDH-mutant gliomas and cholangiocarcinomas.

Sci Adv 2020 Apr 22;6(17):eaaz3221. Epub 2020 Apr 22.

Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Mutations in isocitrate dehydrogenase (IDH) genes occur in multiple cancer types, lead to global changes in the epigenome, and drive tumorigenesis. Yet, effective strategies targeting solid tumors harboring IDH mutations remain elusive. Here, we demonstrate that IDH-mutant gliomas and cholangiocarcinomas display elevated DNA damage. Read More

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http://dx.doi.org/10.1126/sciadv.aaz3221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176409PMC

A novel landscape of nuclear human CDK2 substrates revealed by in situ phosphorylation.

Sci Adv 2020 Apr 17;6(16):eaaz9899. Epub 2020 Apr 17.

Divisions of Clinical Research and Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N. Seattle, WA 98109, USA.

Cyclin-dependent kinase 2 (CDK2) controls cell division and is central to oncogenic signaling. We used an "in situ" approach to identify CDK2 substrates within nuclei isolated from cells expressing CDK2 engineered to use adenosine 5'-triphosphate analogs. We identified 117 candidate substrates, ~40% of which are known CDK substrates. Read More

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http://dx.doi.org/10.1126/sciadv.aaz9899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164936PMC

Negative friction coefficient in microscale graphite/mica layered heterojunctions.

Sci Adv 2020 Apr 17;6(16):eaaz6787. Epub 2020 Apr 17.

State Key Laboratory of Tribology, Tsinghua University, Beijing 100084, China.

The friction of a solid contact typically shows a positive dependence on normal load according to classic friction laws. A few exceptions were recently observed for nanoscale single-asperity contacts. Here, we report the experimental observation of negative friction coefficient in microscale monocrystalline heterojunctions at different temperatures. Read More

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http://dx.doi.org/10.1126/sciadv.aaz6787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164938PMC

A highly sensitive and selective nanosensor for near-infrared potassium imaging.

Sci Adv 2020 Apr 17;6(16):eaax9757. Epub 2020 Apr 17.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.

Potassium ion (K) concentration fluctuates in various biological processes. A number of K probes have been developed to monitor such fluctuations through optical imaging. However, the currently available K probes are far from being sensitive enough in detecting physiological fluctuations in living animals. Read More

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http://dx.doi.org/10.1126/sciadv.aax9757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164935PMC

Self-testing nonprojective quantum measurements in prepare-and-measure experiments.

Sci Adv 2020 Apr 17;6(16):eaaw6664. Epub 2020 Apr 17.

Department of Physics, Stockholm University, S-10691 Stockholm, Sweden.

Self-testing represents the strongest form of certification of a quantum system. Here, we theoretically and experimentally investigate self-testing of nonprojective quantum measurements. That is, how can one certify, from observed data only, that an uncharacterized measurement device implements a desired nonprojective positive-operator valued measure (POVM). Read More

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http://dx.doi.org/10.1126/sciadv.aaw6664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164945PMC

Super-resolution nanoscopy by coherent control on nanoparticle emission.

Sci Adv 2020 Apr 17;6(16):eaaw6579. Epub 2020 Apr 17.

State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871, China.

Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ~50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. Read More

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http://dx.doi.org/10.1126/sciadv.aaw6579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164939PMC

The challenge of ionisation chamber dosimetry in ultra-short pulsed high dose-rate Very High Energy Electron beams.

Sci Rep 2020 Jun 3;10(1):9089. Epub 2020 Jun 3.

Medical Radiation Science, National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 0LW, United Kingdom.

High dose-rate radiotherapy, known as FLASH, has been shown to increase the differential response between healthy and tumour tissue. Moreover, Very High Energy Electrons (VHEEs) provide more favourable dose distributions than conventional radiotherapy electron and photon beams. Plane-parallel ionisation chambers are the recommended secondary standard systems for clinical reference dosimetry of electrons, therefore chamber response to these high energy and high dose-per-pulse beams must be well understood. Read More

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http://dx.doi.org/10.1038/s41598-020-65819-yDOI Listing

Lack of toxicological influences by microsampling (50 µL) from jugular vein of rats in a collaborative 28-day study.

J Toxicol Sci 2020 ;45(6):319-325

National Institute of Health Sciences.

Due to finalization of the ICH S3A Q&A focusing on microsampling, application of microsampling technique to regular non-clinical animal studies is expected for non-clinical safety assessment of pharmaceuticals. In Europe, microsampling from the tail vein or saphenous vein has often been used, whereas sampling from the jugular vein is thought to be more common for non-clinical studies in Japan. Therefore, we assessed the toxicological effects of serial microsampling from the jugular vein of SD rats in a common 28-day study at 4 independent organizations. Read More

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http://dx.doi.org/10.2131/jts.45.319DOI Listing
January 2020

N,P-Doped Carbon Nanodots for Food-Matrix Decontamination, Anticancer Potential, and Cellular Bio-Imaging Applications.

J Biomed Nanotechnol 2020 Mar;16(3):283-303

We report a facile one-step thermal treatment method for the synthesis of biocompatible, fluorescent nitrogen-phosphorus-doped carbon nanodots (NPCDs) as multifunctional agents for the food matrix decontamination, cancer targeting, and cellular bio-imaging. NPCDs exhibit high toxicity towards , as illustrated by fluorescent live-dead cell counting, disruption of membrane permeability/potential, changes in the levels of cellular ions, genetic materials, and proteins, as well as intracellular production of reactive oxygen species. The tryptophan and protein peaks released in NPCDs treated cells contributed to indole ring breathing and correlated with induced cell death. Read More

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http://dx.doi.org/10.1166/jbn.2020.2899DOI Listing

The lipid phosphatase Synaptojanin 1 undergoes a significant alteration in expression and solubility and is associated with brain lesions in Alzheimer's disease.

Acta Neuropathol Commun 2020 Jun 3;8(1):79. Epub 2020 Jun 3.

Laboratory of Histology, Neuroanatomy and Neuropathology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), 808, route de Lennik (Bldg G), 1070, Brussels, Belgium.

Synaptojanin 1 (SYNJ1) is a brain-enriched lipid phosphatase critically involved in autophagosomal/endosomal trafficking, synaptic vesicle recycling and metabolism of phosphoinositides. Previous studies suggest that SYNJ1 polymorphisms have significant impact on the age of onset of Alzheimer's disease (AD) and that SYNJ1 is involved in amyloid-induced toxicity. Yet SYNJ1 protein level and cellular localization in post-mortem human AD brain tissues have remained elusive. Read More

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http://dx.doi.org/10.1186/s40478-020-00954-1DOI Listing

CASS (CyanoAcrylate closure versus Surgical Stripping for incompetent saphenous veins) study: a randomized controlled trial comparing clinical outcomes after cyanoacrylate closure and surgical stripping for the treatment of incompetent saphenous veins.

Trials 2020 Jun 3;21(1):460. Epub 2020 Jun 3.

Department of Surgery, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul National University College of Medicine, 07061 20 Boramae-ro, Dongjak-gu, Seoul, South Korea.

Background: Several modalities are used for the treatment of varicose veins. Open surgical treatment with ligation and stripping of the saphenous vein has been the standard of care for many years. Endovenous thermal ablation has been shown to be a safe and effective alternative with high, long-term, target-vein closure rates. Read More

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http://dx.doi.org/10.1186/s13063-020-04393-0DOI Listing

Eating occasion situational factors and sugar-sweetened beverage consumption in young adults.

Int J Behav Nutr Phys Act 2020 Jun 3;17(1):71. Epub 2020 Jun 3.

Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Australia.

Background: Young adulthood represents an influential transitional period marked by poor dietary habits and excess weight gain. Sugar-sweetened beverages (SSB) are a major source of excess caloric intake among young adults, yet little is known about the correlates of SSB consumption. This study examines the individual and situational correlates of SSB consumption, using real-time assessment of Australian young adults' eating occasions. Read More

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http://dx.doi.org/10.1186/s12966-020-00975-yDOI Listing

Laparoscopic roux-en-Y gastric bypass versus sleeve gastrectomy for teenagers with severe obesity - TEEN-BEST: study protocol of a multicenter randomized controlled trial.

BMC Surg 2020 Jun 3;20(1):117. Epub 2020 Jun 3.

Obesity Center Máxima - Máxima Medical Center, Eindhoven/Veldhoven, The Netherlands.

Background: Recent data support the use of bariatric surgery in adolescents with severe obesity following unsuccessful non-surgical treatments. Sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) have demonstrated reasonably similar weight loss and reduction of obesity related comorbidities in randomized trials in adults. SG has internationally become the most commonly used procedure in adolescents, yet long-term outcome data are lacking. Read More

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http://dx.doi.org/10.1186/s12893-020-00778-9DOI Listing

Decommissioning in a local healthcare system in Sweden: responses to fiscal stress.

BMC Health Serv Res 2020 Jun 3;20(1):501. Epub 2020 Jun 3.

Department of Public Health and Caring Sciences, Health Services Research, Uppsala University, Box 564, 751 22 Uppsala, Sweden.

Background: Drawing on the literature on cutback management, this article deals with healthcare decommissioning in times of austerity. Politicians and decision-makers are typically reluctant to decommission healthcare, and if they do, the public generally reacts strongly towards reductions in service supply. Despite this, comprehensive decommissioning does take place, though empirical knowledge about its effects and economic sustainability is limited. Read More

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http://dx.doi.org/10.1186/s12913-020-05328-wDOI Listing

Total DNA methylation as a biomarker of DNA damage and tumor malignancy in intracranial meningiomas.

BMC Cancer 2020 Jun 3;20(1):509. Epub 2020 Jun 3.

Intraoperative Imaging Unit, Chair and Department of Neurosurgery and Neurotraumatology, Karol Marcinkowski University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.

Background: Meningiomas are the most common primary intracranial tumors in adults. They are initially detected with neuroimaging techniques, but definite histological diagnosis requires tumor surgery to collect tumor tissue. Gross total resection is an optimal and final treatment for the majority of patients, followed by radiotherapy in malignant or refractory cases. Read More

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http://dx.doi.org/10.1186/s12885-020-06982-3DOI Listing

PCR-based screening, isolation, and partial characterization of motile lactobacilli from various animal feces.

BMC Microbiol 2020 Jun 3;20(1):142. Epub 2020 Jun 3.

Department of Agricultural Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.

Background: Most lactobacilli found in animal intestines are generally non-motile, but there are few exceptions. Our previous work showed that Lactobacillus agilis BKN88, which is a highly motile strain originating from a chicken, takes advantage of motility in gut colonization in murine models, and thus motile lactobacilli likely have unique ecological characteristics conferred by motility. However, the ecology and habitat of gut-derived motile lactobacilli are still rarely understood. Read More

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http://dx.doi.org/10.1186/s12866-020-01830-7DOI Listing

Mentalization-based treatments with children and families: A systematic review of the literature.

Clin Child Psychol Psychiatry 2020 Jun 3:1359104520920689. Epub 2020 Jun 3.

Primary Care Psychology, Health Service Executive, Ireland.

Objectives: Over the past decade, mentalization-based treatment (MBT) approaches have been developed specifically for children, adolescents and families. This study provides a systematic review of MBT applicable to both children and families.

Method: Five databases were searched to identify reports of MBT studies published up to February 2020. Read More

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http://dx.doi.org/10.1177/1359104520920689DOI Listing

Orthotopic T-Cell Receptor Replacement-An "Enabler" for TCR-Based Therapies.

Cells 2020 Jun 1;9(6). Epub 2020 Jun 1.

Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), 81675 Munich, Germany.

Natural adaptive immunity co-evolved with pathogens over millions of years, and adoptive transfer of non-engineered T cells to fight infections or cancer so far exhibits an exceptionally safe and functional therapeutic profile in clinical trials. However, the personalized nature of therapies using virus-specific T cells, donor lymphocyte infusion, or tumor-infiltrating lymphocytes makes implementation in routine clinical care difficult. In principle, genetic engineering can be used to make T-cell therapies more broadly applicable, but so far it significantly alters the physiology of cells. Read More

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http://dx.doi.org/10.3390/cells9061367DOI Listing

A Retrosynthetic Analysis of α-Alkenyl-β-Diketones: Regio- and Stereoselective Two-step Synthesis of Highly Arylated Representatives from Acetylenes, Ketones and Acyl Chlorides.

J Org Chem 2020 Jun 3. Epub 2020 Jun 3.

Highly arylated α-alkenyl-β-diketones are synthesized via a two-step sequence consisting of (i) potassium tert-butoxide/DMSO-catalyzed (E)-stereoselective C-H functionalization of ketones with acetylenes followed by (ii) magnesium bromide etherate/DIPEA-soft enolization of the formed β,γ-unsaturated ketones and regioselective acylation with acyl chlorides. The method is compatible with a broad range of substrates and shown to be applicable as an intermediate stage in the construction of arylated heterocycles. Read More

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http://dx.doi.org/10.1021/acs.joc.0c00768DOI Listing

Heme Determination and Quantification Methods and Their Suita-bility for Practical Applications and Every-Day-Use.

Anal Chem 2020 Jun 3. Epub 2020 Jun 3.

Many research institutions, clinical diagnostic laboratories, and blood banks are desperately searching for a possibility to identify and quantify heme in different physiological and pathological settings as well as various research applications. The reasons for this are the toxicity of heme and the fact that it acts as hemolytic and pro-inflammatory molecule. Heme only exerts these severe and undesired effects, when it is not incorporated in hemoproteins. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c00415DOI Listing

Conjugating Micropatches to Living Cells Through Membrane Intercalation.

ACS Appl Mater Interfaces 2020 Jun 3. Epub 2020 Jun 3.

Existing clinical cell therapies, which rely on the use of biological functionalities of living cells, can be further enhanced by conjugating functional particles to the cells to form cell-particle complexes. Disk-shaped microparticles produced by the top-down microfabrication approach possess unique advantages for this application. However, none of current mechanisms for conjugating the microfabricated microparticles to the cells is principally applicable to all types of cells with therapeutic potentials. Read More

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http://dx.doi.org/10.1021/acsami.0c08503DOI Listing

How to catch the N - An inter-species exchange with the right chemistry.

Mol Syst Biol 2020 Jun;16(6):e9514

Max Planck Institute for Plant Breeding Research, Cologne, Germany.

While classical breeding traits have focussed on above-ground tissues, it is becoming clear that underground aspects of plant life are a hidden treasure of tools applicable for resilient crop production. Plants of the legume family develop specialized organs, called nodules, which serve as hosts for Rhizobium bacteroids. A highly specialized symbiotic relationship exists deep inside the nodules. Read More

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http://dx.doi.org/10.15252/msb.20209514DOI Listing