2,014 results match your criteria Appl. Immunohistochem. Mol. Morphol.[Journal]


Tissue Thickness Interferes With the Estimation of the Immunohistochemical Intensity: Introduction of a Control System for Managing Tissue Thickness.

Appl Immunohistochem Mol Morphol 2020 Apr 30. Epub 2020 Apr 30.

Strategic Planning and Molecular Diagnostics, Nichirei Biosciences Inc., Tokyo.

Context And Objective: The conversion of immunohistochemical (IHC) results from 3-dimensional tissue to a 2-dimensional visual image without considering tissue thickness poses a considerable risk of misleading IHC intensities. The present study aimed to clarify whether tissue thickness interferes with the estimation of IHC staining intensity and to introduce a control system to manage it.

Design: We prepared cell lines that are used as controls for human epidermal growth factor receptor 2 (HER2) IHC (MDA-MB-231, MDA-MB-175VII, MDA-MV-453, and SK-BR-3), a polyclonal antibody for HER2, an interferometry to measure the tissue thickness of formalin-fixed paraffin-embedded sections, a microscope with a Halogen or an LED light source, a complementary metal-oxide semiconductor camera in which the output signal can be corrected to γ=1, and a program to estimate color elements (hue, saturation, and luminance). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000859DOI Listing

Aberrant Expression of E-cadherin, N-cadherin, and P-cadherin in Clear Cell Renal Cell Carcinoma: Association With Adverse Clinicopathologic Factors and Poor Prognosis.

Appl Immunohistochem Mol Morphol 2020 Apr 24. Epub 2020 Apr 24.

Department of Pathology, Yeungnam University College of Medicine, Daegu, Republic of Korea.

Objective: Aberrant expression of cadherins is known to be associated with tumor aggression. However, their role in clear cell renal cell carcinoma (CCRCC) is not well elucidated. This study investigated the expression of epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), and placental cadherin (P-cadherin) in CCRCC, and assessed their prognostic significance and clinicopathologic association. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000861DOI Listing

Fatty Acid-binding Protein 4 Expression in Tumor Cells as a Potential Marker for Anaplastic Meningiomas.

Appl Immunohistochem Mol Morphol 2020 Apr 21. Epub 2020 Apr 21.

Department of Pediatric Newborn Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.

Meningiomas are highly vascularized tumors originating from arachnoid cap cells of the leptomeninges. The majority of meningiomas are classified as World Health Organization (WHO) grade I and display a benign clinical course with a low risk of recurrence. In contrast, WHO grade III meningiomas carry a high risk of recurrence and poor prognosis. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000848DOI Listing

Immunohistochemical Validation of Rare Tissues and Antigens With Low Frequency of Occurrence: Recommendations From The Anatomic Pathology Patient Interest Association (APPIA).

Appl Immunohistochem Mol Morphol 2020 Apr 21. Epub 2020 Apr 21.

Agilent Technologies, Santa Clara, CA.

Laboratories worldwide find it challenging to identify enough tissues and cases for verification and validation studies of low-incidence, rare antigens. These antigens have a low frequency of occurrence in the population, or have little or no expression in normal tissues. Validation studies are essential to assure testing standardization before introducing a new instrument, product, or test into the clinical laboratory. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000821DOI Listing

Histologic and Immunohistochemical Evaluation of Infiltrating Inflammatory Cells in Kawasaki Disease Arteritis Lesions.

Appl Immunohistochem Mol Morphol 2020 Apr 21. Epub 2020 Apr 21.

Department of Pathology, Shinshu University School of Medicine, Matsumoto.

Kawasaki disease (KD) is a systemic vasculitis of unknown etiology which predominantly affects medium- and small-sized muscular arteries. Histopathologic studies of KD vasculitis lesions have demonstrated characteristic T cell infiltration and an abundance of CD8 T cells; however, the contribution of cytotoxic lymphocytes to KD vasculitis lesions has not been identified. Here, we histopathologically and immunohistochemically examined infiltrating inflammatory cells, particularly cytotoxic protein-positive cells, such as granzyme B cells and TIA-1 cells, in KD vasculitis lesions. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000860DOI Listing

A Scoring Method for Immunohistochemical Staining on Ki67.

Appl Immunohistochem Mol Morphol 2020 Apr 13. Epub 2020 Apr 13.

Department of Pathology.

An accurate interpretation of immunohistochemistry (IHC) staining results is crucial for precise disease diagnosis. In this study, we present a novel scoring method for interpreting and reporting of IHC staining assay results for the nuclear-type molecule. On the basis of the histologic characteristics, the samples were subdivided into 3 basic structural units and tissue subtypes including covered, mosaic, and mesenchymal subtypes. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000853DOI Listing

Comparison of the Lymph2Cx Assay and Hans Algorithm in Determining the Cell-of-Origin of Diffuse Large B-Cell Lymphomas, Not Otherwise Specified.

Appl Immunohistochem Mol Morphol 2020 Apr 13. Epub 2020 Apr 13.

Department of Pathology and Translational Genomics, Samsung Medical Center.

In the era of precision medicine, accurate and reproducible assignment of cell-of-origin (COO) in diffuse large B-cell lymphoma patients has become important. The Lymph2Cx assay is accurately determining COO by analyzing RNA expression of 20 selected genes while the Hans algorithm based on immunohistochemistry is the most popular method for routine daily diagnosis. However, there are discrepancies between the 2 methods, which need to be evaluated for better correlation. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000843DOI Listing

Hepatocellular Carcinoma Score and Subclassification Into Aggressive Subtypes Using Immunohistochemical Expression of p53, β-Catenin, CD133, and Ki-67.

Appl Immunohistochem Mol Morphol 2020 Apr 13. Epub 2020 Apr 13.

Department of Pathology, Faculty of Medicine.

Hepatocellular carcinoma (HCC) is the most common primary hepatic malignancy in adults. Several studies have classified HCC into molecular subtypes aiming at detecting aggressive subtypes. The aim of the present study was to investigate the role of p53, β-catenin, CD133, and Ki-67 in subclassification of HCC into different aggressive subtypes and the correlation between those markers and the clinicopathologic characteristics of HCC patients. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000840DOI Listing

Problems With the Recommendations for PD-L1 Biomarker Testing.

Appl Immunohistochem Mol Morphol 2020 Apr 10. Epub 2020 Apr 10.

Department of Pathology and Laboratory Medicine, Royal University Hospital.

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http://dx.doi.org/10.1097/PAI.0000000000000856DOI Listing

NordiQC Assessments of Keratin 5 Immunoassays.

Appl Immunohistochem Mol Morphol 2020 Apr 13. Epub 2020 Apr 13.

Departments of Pathology, Aalborg University Hospital.

This paper is number 7 in a series developed through a partnership between ISIMM and NordiQC with the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency testing program. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000855DOI Listing

Expression of Secretory Phospholipase A2 Group IIa in Breast Cancer and Correlation to Prognosis in a Cohort of Advanced Breast Cancer Patients.

Appl Immunohistochem Mol Morphol 2020 Mar 24. Epub 2020 Mar 24.

Departments of Pathology.

Secreted phospholipase A2 group IIa (sPLA2-IIa) has been shown to promote tumor genesis and cell proliferation. The properties of this group of enzymes are utilized in liposomal drug delivery of chemotherapy. sPLA2-IIa is also under investigation as a possible treatment target in itself, and as a prognostic marker. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000854DOI Listing

Cost-effectiveness of a Dual (Immunohistochemistry and Fluorescence In Situ Hybridization) HER2/neu Testing Strategy on Invasive Breast Cancers.

Appl Immunohistochem Mol Morphol 2020 Mar 18. Epub 2020 Mar 18.

Department of Pathology, Anatomic Pathology Division, University of California San Diego Health.

The American Society of Clinical Oncology and the College of American Pathologists recommend that human epidermal growth factor receptor 2 (HER2)/neu status be determined for all invasive breast cancers. Although the most commonly used modalities to determine HER2/neu status, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), generally give concordant results, a small but consistent discordance rate between them has been demonstrated in prior studies. Most institutions in the United States use a "reflex testing strategy" for determining HER2/neu status. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000849DOI Listing

The Utility of Perilipin in Liposarcomas: PLIN1 Differentiates Round Cell Liposarcoma From Other Round Cell Sarcomas.

Appl Immunohistochem Mol Morphol 2020 Mar 18. Epub 2020 Mar 18.

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Liposarcoma is the most common soft tissue sarcoma in adults; however, accurate diagnosis often depends on the use of ancillary molecular testing which can be time consuming and expensive. Myxoid/round cell liposarcoma may be a diagnostic challenge due to the morphologic similarities with other nonadipocytic sarcomas with round cell morphology. Immunohistochemistry may be a helpful adjunct to appropriately triage cases for molecular testing. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000851DOI Listing

PD-L1 Expression Is a Favorable Prognostic Marker in Gastric Carcinoma.

Appl Immunohistochem Mol Morphol 2020 Mar 20. Epub 2020 Mar 20.

School of Medicine, University of Jordan, Amman, Jordan.

Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer-related mortality worldwide. Although multidisciplinary therapeutic strategies have improved treatment outcomes, the overall prognosis for patients with GC remains poor. Recently, immunotherapeutic agents targeting immunosuppressive proteins such as anti-programmed death-1 receptor and anti-programmed death ligand-1 (PD-L1) have emerged as effective treatment options for various cancers, including GC. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000834DOI Listing

The Prognostic Significance of APOBEC3B and PD-L1/PD-1 in Nasopharyngeal Carcinoma.

Appl Immunohistochem Mol Morphol 2020 Mar 20. Epub 2020 Mar 20.

Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.

Purpose: Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3B (APOBEC3B) is a recently discovered protein that is considered important in causing mutations in tumor cell genome bases. Whether APOBEC3B is expressed in nasopharyngeal carcinoma (NPC) still remains unknown. Studies have shown that programmed-cell-death receptor-1 ligand (PD-L1) is highly expressed in NPC, but its clinical significance has not been fully elucidated. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000852DOI Listing

Using Immunohistochemistry Without Linkers to Determine the Optimum Concentrations of Primary Antibodies for Immunofluorescence Staining of Formalin-fixed Paraffin-embedded Tissue Sections.

Appl Immunohistochem Mol Morphol 2020 Mar;28(3):249-257

Haemato-Oncology Group, Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, UK.

The use of immunofluorescence (IF) technique to detect and evaluate expression levels and localization of cellular proteins and other antigens of interest through the antibodies in their cellular or tissue context has become a standard approach among researchers. Optimizing primary antibody concentrations/dilutions is an essential step in the fluorescent antibody staining protocol. The steps in IF staining are similar to those of the immunohistochemistry (IHC) technique. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000718DOI Listing

Significance of Expression of Cancer Stem Cell Markers CD133 and Nestin in Pancreatic Intraepithelial Carcinoma-invasive Adenocarcinoma Sequence.

Appl Immunohistochem Mol Morphol 2020 Mar;28(3):205-212

Department of Pathology, Faculty of Medicine, Tanta University, Tanta, Egypt.

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive malignancies and one of the most leading causes of cancer related deaths. It has a very poor prognosis with high recurrence rate. We investigated the expression of CSC markers (CD133 and nestin) in 100 patients [30 pancreatic intraepithelial tumor cases (PanIN) and 70 PDAC cases] and correlate the expression levels of these markers with clinicopathological data with the aid of Ki67 expression. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000722DOI Listing

EPR17341 and A7H6R pan-TRK Immunohistochemistry Result in Highly Different Staining Patterns In a Series of Salivary Gland Tumors.

Appl Immunohistochem Mol Morphol 2020 Mar 16. Epub 2020 Mar 16.

CHRU Brest, Department of Pathology, Brest.

Patients with NTRK-rearranged tumors can be now treated using anti-TRK-targeted therapies making NTRK testing important for treatment choices in patients with advanced cancers. Pan-TRK immunohistochemistry (IHC) could be a valuable premolecular screening strategy in this field. The choice of 1 IHC method or another requires to investigate for intermethod comparison. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000825DOI Listing

Ki-67 Proliferation Index in Breast Cancer as a Function of Assessment Method: A NordiQC Experience.

Appl Immunohistochem Mol Morphol 2020 Mar 9. Epub 2020 Mar 9.

NordiQC, Institute of Pathology, Aalborg University Hospital.

Immunohistochemical staining for Ki-67 is used to calculate a Ki-67 proliferation index (PI) that carries prognostic and predictive information in various cancers including breast carcinomas. Studies have documented challenges for observers to reproducibly estimate the Ki-67 PI. At present, no international consensus exists concerning scoring method (eg, hotspots vs. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000846DOI Listing

Image Analyses Assessed Cell Lines as Potential Performance Controls of Ki-67 Immunostained Slides.

Appl Immunohistochem Mol Morphol 2020 Mar 9. Epub 2020 Mar 9.

Center for RNA Medicine, Aalborg University Copenhagen, Copenhagen SV, Denmark.

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http://dx.doi.org/10.1097/PAI.0000000000000845DOI Listing

Coexistent Dedifferentiated Endometrioid Carcinoma of the Uterus and Adenocarcinoma of the Bladder in Lynch Syndrome: Case Report and Review of the Literature.

Appl Immunohistochem Mol Morphol 2020 Mar;28(3):e26-e30

Obstetrics and Gynecology, University of Illinois Hospital & Health Sciences System.

Lynch syndrome is an autosomal dominant disorder, caused by an abnormality in DNA mismatch repair genes and characterized by the development of a variety of cancers. Upper urinary tract urothelial carcinoma is well characterized in Lynch syndrome; however, support for the inclusion of bladder urothelial carcinoma is limited, except for MSH2 mutation carriers. Urologic adenocarcinoma has not been documented in Lynch syndrome. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000553DOI Listing

Glycogen-rich Clear Cell Carcinoma of the Breast: A Comprehensive Review.

Appl Immunohistochem Mol Morphol 2020 Mar 11. Epub 2020 Mar 11.

Creighton University School of Medicine, Phoenix, AZ.

Glycogen-rich clear cell carcinoma (GRCC) is a very rare form of primary breast cancer (<0.1% of all breast cancers). It is characterized by the presence of neoplastic cells with a glycogen-abundant clear cytoplasm (the Periodic Acid Schiff-positive, diastase-sensitive). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000850DOI Listing

PTEN Loss and Cyclin A2 Upregulation Define a PI3K/AKT Pathway Activation in Helicobacter pylori-induced MALT and DLBCL Gastric Lymphoma With Features of MALT.

Appl Immunohistochem Mol Morphol 2020 Mar 3. Epub 2020 Mar 3.

Laboratory of Onco-hematology, Faculty of Medecine of Tunis, University of Tunis El Manar.

Helicobacter pylori infection is strongly associated with primary gastric diseases, such as extranodal mucosa-associated lymphoid tissue (MALT) lymphoma, diffuse large B-cell lymphoma (DLBCL) with histologic evidence of MALT origin, and gastric carcinoma. The cytotoxin-associated gene A (CagA) protein behaves as a bacterial oncoprotein, promoting tumorigenesis via dysregulation of the phosphatidylinositol 3-kinase/AKT pathway (PI3K/AKT). We investigated the molecular mechanisms of PI3K/AKT pathway dysregulation in H. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000839DOI Listing

The Immunohistochemical Expression of Programmed Death Ligand 1 (PD-L1) Is Affected by Sample Overfixation.

Appl Immunohistochem Mol Morphol 2020 Mar 3. Epub 2020 Mar 3.

Pathology Department, Germans Trias i Pujol Hospital and Institute, Faculty of Medicine and Health Sciences, Universitat Autònoma de Barcelona.

Humanized antibodies targeting programmed death receptor 1 (PD-1) or its ligand (PD-L1) have been approved for the treatment of different cancers. Some of these antibodies show a correlation between the tissue expression of PD-L1 and response. Evaluation of PD-L1 expression presents multiple challenges, but some preanalytical issues such as tissue fixation have been scarcely evaluated. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000847DOI Listing

HER2-positive Metastatic Melanoma: A Cautionary Tale!

Appl Immunohistochem Mol Morphol 2020 Feb 25. Epub 2020 Feb 25.

Department of Pathology and Laboratory Medicine, The Ohio State University Wexner Medical Center, Columbus, OH.

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http://dx.doi.org/10.1097/PAI.0000000000000836DOI Listing
February 2020

Prognostic and Predictive role of Excision Repair Cross-complementation Group 1 and Thymidylate Synthase in Colorectal Carcinoma Patients Received FOLFOX Chemotherapy: An Immunohistochemical Study.

Appl Immunohistochem Mol Morphol 2020 Mar 2. Epub 2020 Mar 2.

Pathology Department.

Background And Study Aims: We aim to determine the frequency of thymidylate synthase (TS) and excision repair cross-complementation group 1 (ERCC-1) immunohistochemical (IHC) expression and its relationship with clinicopathologic variables in colorectal carcinoma (CRC) patients. In addition, we aim to assess the correlation between TS and ERCC-1 expression and the response of these cases to oxaliplatin and 5-fluorouracil chemotherapy (FOLFOX).

Patients And Methods: Fifty-one CRC patients were prepared for IHC analysis of ERCC-1 and TS protein expression. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000841DOI Listing

Is CSF1R Expression Localization Crucial for its Prognostic Value in Colorectal Cancer?

Appl Immunohistochem Mol Morphol 2020 Mar 2. Epub 2020 Mar 2.

Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, Shenyang City, China.

The colony-stimulating factor 1 receptor (CSF1R) is commonly known as a transmembrane receptor on tumor-associated macrophages, which are essential in the tumor microenvironment. However, the expression pattern and prognosis of CSF1R are still unknown in colorectal cancer (CRC) and are still controversial among various cancers. To clarify the expression pattern and prognosis of CSF1R in CRC, we performed immunohistochemistry on 332 CRC tissue and 283 nontumorous adjacent tissues. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000844DOI Listing

Ki67 in Gleason Pattern 3 as a Marker of the Presence of Higher-Grade Prostate Cancer.

Appl Immunohistochem Mol Morphol 2020 Feb 26. Epub 2020 Feb 26.

Department of Medicine and Surgery, University of Salerno, Baronissi.

Purpose: Prostate biopsies may undergrade up to half of all prostate cancers (PCs), delaying definitive treatment by up to 3 years. One cause of undergrading is the partial sampling inherent in the technique. Because of this, a prostate biopsy that appears to be Gleason 3+3=6 may come either from a true 3+3=6 tumor or from a higher-grade tumor that has been sampled only partially. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000835DOI Listing
February 2020

Problems With the Recommendations for PD-L1 Biomarker Testing.

Authors:
Robert Wolber

Appl Immunohistochem Mol Morphol 2020 Feb 11. Epub 2020 Feb 11.

Lions Gate Hospital, North Vancouver BC, Canada.

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http://dx.doi.org/10.1097/PAI.0000000000000838DOI Listing
February 2020

Micropapillary Thyroid Carcinoma Arising in Mature Cystic Teratoma of the Ovary Without Evidence of Stroma Ovarii.

Appl Immunohistochem Mol Morphol 2020 Feb 11. Epub 2020 Feb 11.

Department of Anatomic Pathology Aurora Diagnostics/Sonic Healthcare Austin, TX.

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http://dx.doi.org/10.1097/PAI.0000000000000837DOI Listing
February 2020

Is Sclerosing Angiomatoid Nodular Transformation an IgG4-associated Sclerosing Disease?

Appl Immunohistochem Mol Morphol 2020 02;28(2):e18-e20

Department of Biliary Surgery.

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http://dx.doi.org/10.1097/PAI.0000000000000634DOI Listing
February 2020

Androgen Receptor is Mostly Not Expressed in Gastric Cancers.

Appl Immunohistochem Mol Morphol 2020 02;28(2):e17-e18

Department of Pathology, Ajou University School of Medicine, Suwon, Republic of Korea.

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http://dx.doi.org/10.1097/PAI.0000000000000559DOI Listing
February 2020

ACTH-Cell Pituitary Adenoma With Signet Ring Cells: A Rare Case Report and Review of The Literature.

Appl Immunohistochem Mol Morphol 2020 02;28(2):e13-e16

Neurosurgery, Kocaeli University School of Medicine, Kocaeli, Turkey.

Here, we present a case of pituitary adenoma producing adrenocorticotropic hormone (ACTH) in a 19-year-old woman. The patient was admitted to neurosurgery clinic because of a headache and decreased visual acuity. Transsphenoidal resection was performed. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000639DOI Listing
February 2020

Beneficial Effect of Heat-induced Antigen Retrieval in Immunocytochemical Detection of Intracellular Antigens in Alcohol-fixed Cell Samples.

Appl Immunohistochem Mol Morphol 2020 02;28(2):166-174

Departments of Histology and Embryology.

Immunohistochemistry and immunocytochemistry (ICC) play an irreplaceable role in research and diagnostics. It is well known that antigen retrieval (AR) can, as a technique, have beneficial outcomes on immunohistochemistry results when using formalin-fixed, paraffin-embedded tissue samples. The main purpose of AR is to break protein crosslinks which are formed during formalin fixation. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000689DOI Listing
February 2020

PD-L1 Copy Number Variation Does Not Correlate With PD-L1 Expression or Response to Anti-PD-1 Immunotherapy In Patients With Advanced Melanomas.

Appl Immunohistochem Mol Morphol 2020 02;28(2):161-165

Department of Pathology, CHRU Brest.

Predicting the response to PD-1/PD-L1 immune checkpoint blockade in patients with metastatic melanoma remains challenging. In this study, we have investigated for the relationships between PD-L1 expression, PD-L1 copy number variations, and the response to anti-PD-1 therapies. We studied the formalin-fixed paraffin-embedded tumor samples of 36 patients with metastatic melanoma using PD-L1 immunohistochemistry (IHC) and PD-L1/chromosome 9 fluorescent in situ hybridization (FISH). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000712DOI Listing
February 2020

Novel Interplay Between Sonic Hedgehog and Transforming Growth Factor-β1 in Human Nonalcoholic Steatohepatitis.

Appl Immunohistochem Mol Morphol 2020 02;28(2):154-160

Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology.

Nonalcoholic steatohepatitis (NASH) has the potential to progress to fibrosis, cirrhosis, and hepatocellular carcinoma. Upregulation of sonic hedgehog (Shh) has been documented in development of NASH through sustained cell stress. At the same time, transforming growth factor-β1 (TGF-β1), which is a central element in fibrogenic reactions in various diseases and sites, has been reported to be associated with hepatic inflammation and fibrotic reaction. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000724DOI Listing
February 2020

Immunostaining Patterns of Posttransplant Liver Biopsies Using 2 Anti-C4d Antibodies.

Appl Immunohistochem Mol Morphol 2020 02;28(2):146-153

Department of Pathology and Laboratory Medicine, University of California at Los Angeles.

Histopathologic diagnosis of antibody-mediated rejection in posttransplant liver biopsies is challenging. The recently proposed diagnostic criteria by the Banff Working Group on Liver Allograft Pathology require positive C4d immunohistochemical staining to establish the diagnosis. However, the reported C4d staining patterns vary widely in different studies. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000723DOI Listing
February 2020

Complement Component C4d Deposition in the Placenta of Preeclampsia Patients and Renal Glomeruli in 1 Postpartum Renal Biopsy.

Appl Immunohistochem Mol Morphol 2020 02;28(2):139-145

Departments of Pathology.

Background: The classic disorder of placental malperfusion is preeclampsia (PE), in which the kidney is also a target organ, leading to renal dysfunction. Although the precise pathogenesis of PE is unknown, increasing evidence suggests that PE is associated with complement dysregulation. The maternal immune response to an allogenic fetus and excessive activation of the complement system may both be involved in the pathogenesis of PE. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000755DOI Listing
February 2020

SERINE-910 Phosphorylated Focal Adhesion Kinase Expression Predicts Better Overall and Disease-free Survival in Melanoma.

Appl Immunohistochem Mol Morphol 2020 02;28(2):130-138

Department of Pathology, Albany Medical College, MC-81, Albany, NY.

Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase that mediates multiple cellular functions such as survival, invasion, and migration. FAK has been found to be over-expressed in various human cancers, including melanoma. FAK molecule has several tyrosine, serine, and threonine phosphorylation sites which have an important regulatory role. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000744DOI Listing
February 2020

Application of Chromosome Microarray Analysis for the Differential Diagnosis of Low-grade Renal Cell Carcinoma With Clear Cell and Papillary Features.

Appl Immunohistochem Mol Morphol 2020 02;28(2):123-129

Department of Pathology.

Clear cell renal cell carcinoma (ccRCC) and papillary renal cell carcinoma (pRCC) are the 2 most common RCCs. However, some RCCs can have both clear cell and papillary features, including clear cell papillary RCC (ccpRCC). They can be a diagnostic challenge in daily practice. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000704DOI Listing
February 2020

Differentially Expressed Genes in Matched Normal, Cancer, and Lymph Node Metastases Predict Clinical Outcomes in Patients With Breast Cancer.

Appl Immunohistochem Mol Morphol 2020 02;28(2):111-122

Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan, Republic of Korea.

Genome-wide screening of transcriptional changes among normal, cancer, and nodal metastases provides insights into the molecular basis of breast cancer (BC) progression and metastasis. To identify transcriptional changes and differentially expressed genes (DEGs) in the metastatic progression of BC and to determine the prognostic role of these DEGs in clinical outcome, we compared transcriptome profiling in matched normal, cancer, and lymph node metastatic tissues of 7 patients with estrogen receptor-positive, HER2-negative BC by using massive parallel RNA sequencing. The global profiles of gene expression in cancer and nodal metastases were highly correlated (r=0. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028469PMC
February 2020

Clinical Significance of CBS and CCL21 in Gallbladder Adenocarcinomas and Squamous Cell/Adenosquamous Carcinomas.

Appl Immunohistochem Mol Morphol 2020 02;28(2):103-110

Department of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

Gallbladder cancer (GBC) is a rare disease with high mortality. However, no biomarkers for the carcinogenesis, progression, prognosis, and early diagnosis are clinically available. This study investigated the expressions of cystathionine-β-synthase (CBS) and C-C chemokine receptor 7 (CCR7) protein and their clinical and pathologic significances in gallbladder squamous cell/adenosquamous carcinomas (SC/ASC) and adenocarcinomas (AC). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000705DOI Listing
February 2020

Immunohistochemistry in the Detection of Early Myocardial Infarction: Systematic Review and Analysis of Limitations Because of Autolysis and Putrefaction.

Appl Immunohistochem Mol Morphol 2020 02;28(2):95-102

Department of Legal and Forensic Medicine, University of Genova, Via De Toni 12, Genova, 16132, Italy.

The postmortem diagnosis of acute myocardial infarction is one of the main problems in forensic practice, especially in cases in which death occurs soon after (from minutes to a few hours) the onset of the ischemic damage. Several authors have highlighted the possibility to overcome the limits of conventional histology in this diagnosis by utilizing immunohistochemistry. In the present research, we examined over 30 scientific studies and picked out over 20 main immunohistochemical antigens analyzed with a view to enabling the rapid diagnosis of early myocardial infarction. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000688DOI Listing
February 2020

Milestones in Immunohistochemistry and Molecular Morphology.

Authors:
Clive R Taylor

Appl Immunohistochem Mol Morphol 2020 02;28(2):83-94

Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA.

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http://dx.doi.org/10.1097/PAI.0000000000000833DOI Listing
February 2020

Prognostic and Predictive Role of CXC Chemokine Receptor 4 in Metastatic Colorectal Cancer Patients.

Appl Immunohistochem Mol Morphol 2020 Jan 23. Epub 2020 Jan 23.

Department of Abdominal Oncology, SSD-Innovative Therapies for Abdominal Metastases.

Background: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide. About 30% of patients present with metastatic disease involving predominantly the liver and a similar percentage will develop distant metastases later after removal of the primary tumor. In metastatic CRC, chemotherapies and biological drugs have prolonged survival for up to 30 months. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000828DOI Listing
January 2020

Comparison of PD-L1 Expression Using 2 Validated PD-L1 IHC 22C3 pharmDx Methods in Non-Small Cell Lung Cancer in a Routine Hospital Setting.

Authors:
Birgit G Skov

Appl Immunohistochem Mol Morphol 2019 Dec 24. Epub 2019 Dec 24.

Department of Pathology, University Hospital, Rigshospitalet, Copenhagen, Denmark.

Concordance between 3 validated, commercial programmed cell death ligand 1 (PD-L1) assays and their associated platforms (PD-L1 IHC 22C3 pharmDx Autostainer Link 48, PD-L1 IHC 28-8 pharmDx Autostainer Link 48, and Ventana SP263) has been demonstrated in non-small cell lung cancer. No comparison studies exist for IHC 22C3 pharmDx on the Dako Omnis platform. PD-L1 scoring can be challenging and time-consuming, but no quantitative data exist. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000827DOI Listing
December 2019

Combined Application of Immunohistochemistry and Warthin-Starry Silver Stain on the Pathologic Diagnosis of Cat Scratch Disease.

Appl Immunohistochem Mol Morphol 2020 Jan 6. Epub 2020 Jan 6.

Department of Pathology, The Second Xiangya Hospital, Central South University.

Purpose: Cat scratch disease (CSD) is an infectious disorder caused primarily by the bacterium Bartonella henselae (B. henselae). Immunohistochemistry (IHC) and Warthin-Starry silver stain (WS) are considered to be indispensable to diagnose CSD in combination with morphologic characteristics. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000829DOI Listing
January 2020

Immunohistochemical Expression Analysis of EpCAM in Hepatoblastomas.

Appl Immunohistochem Mol Morphol 2019 Dec 4. Epub 2019 Dec 4.

Department of Pathology, UPMC.

The epithelial cell adhesion molecule (EpCAM) is involved in oncogenesis of hepatoblastomas (HBs). Prior genomic profiling studies showed higher EpCAM expression and worse prognosis in HBs containing primitive histotypes, however, this has not been fully addressed from an immunohistochemical perspective. Our goal is to characterize differential EpCAM immunohistochemistry (EpCAM-IHC) among HBs histotypes. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000826DOI Listing
December 2019

Round Robin Evaluation of MET Protein Expression in Lung Adenocarcinomas Improves Interobserver Concordance.

Appl Immunohistochem Mol Morphol 2019 Nov 22. Epub 2019 Nov 22.

Department of Medicine, Division of Medical Oncology.

Introduction: Overexpression of the mesenchymal-epithelial transition (MET) receptor, a receptor tyrosine kinase, can propel the growth of cancer cells and portends poor prognoses for patients with lung cancer. Evaluation of MET by immunohistochemistry is challenging, with MET protein overexpression varying from 20% to 80% between lung cancer cohorts. Clinical trials using MET protein expression to select patients have also reported a wide range of positivity rates and outcomes. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242128PMC
November 2019

Insulinoma-associated Protein 1 (INSM1) Expression in Small Cell Neuroendocrine Carcinoma of the Urinary Tract.

Appl Immunohistochem Mol Morphol 2019 Dec 23. Epub 2019 Dec 23.

Department of Pathology and Laboratory Medicine, Brown University/Lifespan, Providence, RI.

Clinical guidelines state that neoadjuvant chemotherapy should be administered before surgery in muscle invasive urinary bladder small cell neuroendocrine carcinoma. Recently described marker insulinoma-associated protein 1 (INSM1) has been reported to be sensitive and specific for neuroendocrine differentiation, however, its efficacy in urinary tract small cell carcinoma is not well established. This study examines immunohistochemical expression of INSM1 on whole tissue sections of urinary tract small cell neuroendocrine carcinoma and compares INSM1 expression with established neuroendocrine markers. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000824DOI Listing
December 2019