1,894 results match your criteria Appl. Immunohistochem. Mol. Morphol.[Journal]


Composite Lymphoma Containing Mantle Cell and Peripheral T-cell Lymphoma, Not Otherwise Specified: A Report of 2 Cases Treated With Up-front Autologous Stem Cell Transplantation.

Appl Immunohistochem Mol Morphol 2019 Apr 9. Epub 2019 Apr 9.

Departments of Hematology.

We report 2 cases of composite lymphoma comprising mantle cell lymphoma and peripheral T-cell lymphoma, not otherwise specified, a rare association that has only been reported twice in the literature. In case 1, a 64-year-old woman presented with massive splenomegaly and lymphadenopathy. Immunohistochemical studies of the lymph node biopsy suggested the presence of 2 lymphomas, a predominant component of a peripheral T-cell lymphoma, not otherwise specified and an in situ mantle cell neoplasia. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000769DOI Listing
April 2019
1 Read

Immunohistochemistry: Growing Pains, From a Stain to an Assay.

Authors:
Clive R Taylor

Appl Immunohistochem Mol Morphol 2019 Apr 5. Epub 2019 Apr 5.

Keck School of Medicine, University of Southern California, Los Angeles, CA.

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http://dx.doi.org/10.1097/PAI.0000000000000770DOI Listing

IgG4-positive Cell Quantification Distinguishes Between Inflammatory and Noninflammatory Diseases of the Orbit.

Appl Immunohistochem Mol Morphol 2019 Apr 3. Epub 2019 Apr 3.

Departments of Ophthalmology.

IgG4-related ophthalmic disease (IgG4-ROD) is a rare inflammatory disorder often refractory to corticosteroids and prone to recurrence. IgG4-ROD may frequently lack the characteristic histopathological features seen in other organs. Thus, the criteria for diagnosis of IgG4-ROD relies on elevated IgG4 cells seen on biopsied tissue. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000767DOI Listing

Combined Plasmatic and Tissue Approach to Membranous Nephropathy-Proposal of a Diagnostic Algorithm Including Immunogold Labelling: Changing the Paradigm of a Serum-based Approach.

Appl Immunohistochem Mol Morphol 2019 Mar 28. Epub 2019 Mar 28.

Department of Medicine and Surgery, Pathology, San Gerardo Hospital.

Membranous nephropathy represents the most frequent cause of nephrotic syndrome in the adult, leading to end-stage renal disease in one third of all the patients. In the last years, the discovery of circulating autoantibodies against phospholipase A2 receptor 1 (PLA2R) and thrombospondin type-1 containing 7A domain (THSD7A), shed light on the pathogenesis of idiopathic forms, being responsible for 70% and 3% of all the cases, respectively. These identifications allowed the development of serological and histologic tests to detect autoantibodies and relative targets for diagnostic and prognostic purposes. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000753DOI Listing
March 2019
1 Read

Estrogen Receptor-α Quantification in Breast Cancer: Concordance Between Immunohistochemical Assays and mRNA-In Situ Hybridization for ESR1 Gene.

Appl Immunohistochem Mol Morphol 2019 Mar 27. Epub 2019 Mar 27.

Nordic Immunohistochemical Quality Control (NordiQC).

Immunohistochemical (IHC) quantification of estrogen receptor-α (ER) is used for assessment of treatment regimen in breast cancer. Different ER IHC assays may produce diverging results, because of different antibody clones, protocols, and stainer platforms. Objective tissue-based techniques to assess sensitivity and specificity of IHC assays are therefore needed. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000760DOI Listing

A Biotin Tagging Immunoelectron Microscopy for Paraffin-embedded Sections.

Appl Immunohistochem Mol Morphol 2019 Mar 27. Epub 2019 Mar 27.

Department of Diagnostic Pathology, Faculty of Medicine, Oita University, Yufu, Japan.

We herein introduce a novel method of biotin tagging immunoelectron microscopy for formalin-fixed, paraffin-embedded sections. This method was developed to utilize the antigenicity of biotin on epoxy-embedded ultrathin sections that could readily be recovered by a previously established antigen retrieval method as most monoclonal antibodies failed to recognize their targets by immunoelectron microscopy following antigen retrieval. The biotin tagging method was composed of preembedding immunostaining, epoxy-embedding and sectioning, and postembedding immunostaining steps. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000735DOI Listing

Screening for NTRK-rearranged Tumors Using Immunohistochemistry: Comparison of 2 Different pan-TRK Clones in Melanoma Samples.

Appl Immunohistochem Mol Morphol 2019 Mar 27. Epub 2019 Mar 27.

Department of Pathology, CHRU Brest, Brest.

NTRK-rearranged tumors could be treated using promising anti-TRK-targeted therapies in patients with advanced cancers including melanomas. Different targeted therapies are being developed together with different screening strategies including pan-TRK immunohistochemistry (IHC) as first-line screening strategies. In this technical study, we compared 2 pan-TRK IHC (using A7H6R and EPR17341 clones) in tumor samples of patients with advanced melanomas. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000708DOI Listing

Expression of the Receptor for Hyaluronic Acid-Mediated Motility (RHAMM) in Endometrial Cancer is Associated With Adverse Histologic Parameters and Tumor Progression.

Appl Immunohistochem Mol Morphol 2019 Mar 26. Epub 2019 Mar 26.

Departments of Pathology and Laboratory Medicine.

Endometrial cancer is one of the most common gynecologic malignancies worldwide. Only 2 agents have been approved by Food and Drug Administration for endometrial cancer since 1971. There is a need to identify molecular targets to treat advanced endometrial cancer. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000763DOI Listing
March 2019
1 Read

Gene Expression Profiling Revealed 2 Types of Bronchial Basal Cell Hyperplasia and Squamous Metaplasia With Different Progression Potentials.

Appl Immunohistochem Mol Morphol 2019 Mar 20. Epub 2019 Mar 20.

Tomsk National Research Medical Center.

The premalignant process preceding squamous cell lung cancer is not inevitable; it can stop at any of the bronchial lesions: basal cell hyperplasia (BCH), squamous metaplasia (SM), and dysplasia and then progress or regress. At present, the mechanisms underlying the progression of the bronchial lesions remain undefined. Previously, we hypothesized that bronchial lesions that presented individually or combined with each other in the bronchi of lung cancer patients mirror the different "scenarios" of the premalignant process: individual BCH-the stoppage at the stage of hyperplasia, BCH plus SM-the progression of hyperplasia to metaplasia, and SM plus dysplasia-the progression of metaplasia to dysplasia. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000762DOI Listing

Metastatic Renal Cell Carcinoma to the Brain: A Contemporary Clinicopathologic Analysis With Comparison of Immunohistochemical Profiles to Selected Primary Brain Tumors With Clear Cell Features.

Appl Immunohistochem Mol Morphol 2019 Mar 13. Epub 2019 Mar 13.

Departments of Pathology.

Brain metastases from renal cell carcinoma (RCC) are associated with significant morbidity and mortality. However, there are only few large series in the pathology literature specifically analyzing the clinicopathologic and immunohistochemical features in comparison with primary brain tumors with clear cell features. We identified 34 cases of metastatic RCC to the brain from the Urologic Pathology and Neuropathology files of 2 institutions between 2000 and 2018. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000759DOI Listing
March 2019
1 Read

Immunohistochemical SATB2 Expression in Breast Carcinomas: A Note of Caution.

Authors:
David Creytens

Appl Immunohistochem Mol Morphol 2019 Mar 6. Epub 2019 Mar 6.

Department of Pathology.

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http://dx.doi.org/10.1097/PAI.0000000000000757DOI Listing

The Immunohistochemical Pattern of Epithelial-Mesenchymal Transition Markers In Endometrial Carcinoma.

Appl Immunohistochem Mol Morphol 2019 Feb 28. Epub 2019 Feb 28.

Pathology.

The majority of endometrial carcinoma are diagnosed at an early stage and exhibit a favorable prognosis. However, 10% to 15% of ECs recur and the majority are type II tumors which are high-grade carcinomas. The epithelial-mesenchymal transition (EMT) has been considered as a fundamental step for the development of the invasive phenotype of cancer cells. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000754DOI Listing
February 2019
3 Reads

A Root Cause Analysis Into the High Error Rate in Clinical Immunohistochemistry.

Authors:
Steven A Bogen

Appl Immunohistochem Mol Morphol 2019 Feb 22. Epub 2019 Feb 22.

Department of Pathology & Laboratory Medicine, Tufts Medical Center and MDP LLC, Boston, MA.

The field of Clinical Immunohistochemistry (IHC) is beset with a high error rate, an order of magnitude higher than in other types of clinical laboratory testing. Despite the many improvements in the field, these errors have persisted over the last 2 decades. The improvements over the years include an extensive literature describing the potential causes of errors and how to avoid them. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000750DOI Listing
February 2019

Immunostaining Patterns of Posttransplant Liver Biopsies Using 2 Anti-C4d Antibodies.

Appl Immunohistochem Mol Morphol 2019 Feb 22. Epub 2019 Feb 22.

Department of Pathology and Laboratory Medicine, University of California at Los Angeles.

Histopathologic diagnosis of antibody-mediated rejection in posttransplant liver biopsies is challenging. The recently proposed diagnostic criteria by the Banff Working Group on Liver Allograft Pathology require positive C4d immunohistochemical staining to establish the diagnosis. However, the reported C4d staining patterns vary widely in different studies. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000723DOI Listing
February 2019
1 Read

Diagnostic Accuracy in Fit-for-Purpose PD-L1 Testing.

Appl Immunohistochem Mol Morphol 2019 Apr;27(4):251-257

Canadian Immunohistochemistry Quality Control.

PD-L1 testing by immunohistochemistry (IHC) has presented significant challenges not only for clinical laboratories, but also for external quality assurance (EQA) entities that provide proficiency testing (PT) for clinical laboratories. Canadian Immunohistochemistry Quality Control (CIQC) has used educational runs to explore approaches to sample design and analysis of results that would enhance patient safety. As PT for predictive biomarkers requires modeling at every level (design of the run, assessment of the run, and reporting of "pass" or "fail") based on "fit-for-purpose" principles, CIQC has applied those principles to PD-L1 PT runs. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000734DOI Listing
April 2019
4 Reads

Frequent CTNNB1 or PIK3CA Mutations Occurred in Endometrial Endometrioid Adenocarcinoma With High Levels of Microsatellite Instability and Loss of MSH2/MSH6 Expression.

Appl Immunohistochem Mol Morphol 2019 Feb 15. Epub 2019 Feb 15.

Department of Pathology, Graduate Institute of Pathology.

Background: DNA mismatch repair (MMR) proteins form 2 heterodimers-MutSα formed by MSH2 and MSH6, and MutLα by MLH1 and PMS2. In endometrial endometrioid adenocarcinomas, cases with MMR protein defect also usually harbor other recurrent genetic mutations of the neoplasm. However, it remains unknown whether defects of the 2 functionally different heterodimers are linked to mutations in different genes. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000749DOI Listing
February 2019
2 Reads

Primary Renal Synovial Sarcomas: PAX 8 Immunostaining and Unusual Molecular Findings.

Appl Immunohistochem Mol Morphol 2019 Feb 15. Epub 2019 Feb 15.

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN.

Synovial sarcoma (SS) is a high-grade sarcoma that rarely involves the kidney. Eleven renal SS cases were collected in our institution. Immunostaining features and molecular changes of renal SS were further elucidated in this study. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000736DOI Listing
February 2019
2 Reads

Incidental Finding of Gastrointestinal Stromal Tumor in the Appendix.

Appl Immunohistochem Mol Morphol 2019 Feb 11. Epub 2019 Feb 11.

Departments of Pathology and Radiology, Hôpital Saint-Antoine AP-HP, Paris, France.

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http://dx.doi.org/10.1097/PAI.0000000000000747DOI Listing
February 2019

Immunohistochemical Expression of CDX2 and SATB2 in Primary Breast Carcinomas.

Appl Immunohistochem Mol Morphol 2019 Feb 6. Epub 2019 Feb 6.

Department of Pathology, University of Florida, Gainesville, FL.

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http://dx.doi.org/10.1097/PAI.0000000000000748DOI Listing
February 2019

Impact of Pre-Analytical Conditions on the Antigenicity of Lung Markers: ALK and MET.

Appl Immunohistochem Mol Morphol 2019 Feb 4. Epub 2019 Feb 4.

Roche Tissue Diagnostics (Ventana Medical Systems Inc.), Tucson, AZ.

Diagnostic assays for molecular alterations highly correlated with prognosis, predictive efficacy or safety of therapeutics are valuable clinical tools and in some cases approved as companion diagnostics (CDx) by the Federal Food and Drug Administration. For example, assays that determine echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) translocation status have been approved as CDx assay for therapies that target this molecular alteration. Characterizing the parameters that may compromise diagnostic accuracy for molecular biomarkers is critical for optimal patient care. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000730DOI Listing
February 2019
4 Reads

Novel Interplay Between Sonic Hedgehog and Transforming Growth Factor-β1 in Human Nonalcoholic Steatohepatitis.

Appl Immunohistochem Mol Morphol 2019 Feb 1. Epub 2019 Feb 1.

Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology.

Nonalcoholic steatohepatitis (NASH) has the potential to progress to fibrosis, cirrhosis, and hepatocellular carcinoma. Upregulation of sonic hedgehog (Shh) has been documented in development of NASH through sustained cell stress. At the same time, transforming growth factor-β1 (TGF-β1), which is a central element in fibrogenic reactions in various diseases and sites, has been reported to be associated with hepatic inflammation and fibrotic reaction. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000724DOI Listing
February 2019
4 Reads

Validation and Proficiency Testing of Biomarker Immunohistochemistry: Diagnostic Accuracy "Fit for Purpose" in a 3D World.

Authors:
Paul E Swanson

Appl Immunohistochem Mol Morphol 2019 Apr;27(4):247-250

University of Washington School of Medicine, Seattle, WA.

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http://dx.doi.org/10.1097/PAI.0000000000000746DOI Listing

Molecular Testing for EGFR Mutations and ALK Rearrangements in the Cytological Specimens From the Patients With Non-Small Cell Lung Cancer.

Appl Immunohistochem Mol Morphol 2019 Feb;27(2):119-124

Department of Pathology, Indiana University School of Medicine, Indianapolis, IN.

Objective: The aim of this study was to investigate epidermal growth factor receptor (EGFR) gene mutations and anaplastic lymphoma kinase (ALK) gene rearrangements using cytological specimens from the patients with a diagnosis of primary or metastatic lung non-small cell carcinoma.

Materials And Methods: A total 307 cases were submitted for EGFR mutational analysis and 265 cases for ALK analysis. The cytological specimen sources included lung, lymph node, liver, bone, adrenal gland, mesentery mass, and body fluids/bronchial brushing. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000701DOI Listing
February 2019
7 Reads

Loss of Heterozygosity (LOH) at 17p13 and 22q13 are Shared by Breast and Thyroid Carcinomas for Metastasis.

Appl Immunohistochem Mol Morphol 2019 Feb;27(2):e16-e19

Department of Pathology, Allegheny General Hospital, Allegheny Health Network, Pittsburgh, PA.

Many genomic mutations have been identified to be related to the metastasis of malignancies from various primary sites. In this study, we attempted to identify the loss of heterozygosity (LOH) that might be involved in metastasis of breast ductal carcinoma (BDC) and papillary thyroid carcinoma (PTC). We retrieved 14 BDC cases with metastasis and 19 BDC cases without metastasis as well as 12 PTC cases with metastasis and 14 PTC cases without metastasis. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000630DOI Listing
February 2019
1 Read

Normal Expression of SRY-related HMG-BOX Gene 10 (SOX-10) in Recent and Old Cutaneous Scars is a Potential Mimicker of Desmoplastic Malignant Melanoma.

Appl Immunohistochem Mol Morphol 2019 Jan 21. Epub 2019 Jan 21.

Arkadi M. Rywlin M.D. Department of Pathology and Laboratory Medicine, Mount Sinai Medical Center, Miami Beach, FL.

Desmoplastic malignant melanoma (DMM) is an amelanotic spindle cell proliferation that can be mistaken for a cutaneous scar. The distinction can be difficult in reexcisions because DMM is negative for conventional melanoma markers such as HMB-45 and Melan-A, and scars may be positive for S-100 protein and SOX-10. We compare a total of 12 DMM cases with 8 reexcision and 35 old non-reexcision cutaneous scars using SOX-10 immunohistochemical stains. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000729DOI Listing
January 2019
12 Reads

Collision Tumor of Urothelial Cell Carcinoma and Clear Cell Renal Cell Carcinoma: A Case Report and Review of the Contemporary Literature.

Appl Immunohistochem Mol Morphol 2019 Jan 21. Epub 2019 Jan 21.

Department of Histopathology, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, United Kingdom.

We present a case of synchronous renal cell carcinoma and urothelial carcinoma. Our case was a true collision tumor and both components were high grade. Metastasis to the adrenal gland by the urothelial carcinoma was observed. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000728DOI Listing
January 2019
1 Read

The Application of FOXO1A Expression Predicts Aggressive Behavior and Poor Prognosis in Gliomas.

Appl Immunohistochem Mol Morphol 2019 Jan 19. Epub 2019 Jan 19.

Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.

Forkhead box, class O, 1A (FOXO1A) is an important factor related to some human malignancies. We tested the association between FOXO1A cytoplasmic expression and World Health Organization grading system in primary brain tumors (PBTs). First of all, Western blot analysis was also performed in normal brain tissue and U87MG, LN229, GBM8401, and U118MG glioma cell lines protein lysates. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000710DOI Listing
January 2019

A Comparison of E6H4 and G175-405 p16-specific Monoclonal Antibodies in Oropharyngeal Squamous Cell Carcinoma.

Appl Immunohistochem Mol Morphol 2019 Jan 19. Epub 2019 Jan 19.

Department of Surgery, Division of Otolaryngology-Head & Neck Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI.

The purpose of this investigation is to directly compare G175-405 and E6H4 p16-specific antibodies as immunomarkers of HPV-driven oropharyngeal carcinoma. The investigators designed a retrospective analysis using specimens from an archived tissue bank with known in situ hybridization and polymerase chain reaction status for HPV DNA. Fifty randomly selected oropharyngeal specimens were evaluated with both the G175-405 and E6H4 p16-specific monoclonal antibodies. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000741DOI Listing
January 2019
1 Read

STAT6 Expression in Solitary Fibrous Tumor and Histologic Mimics: a Single Institution Experience.

Appl Immunohistochem Mol Morphol 2019 Jan 19. Epub 2019 Jan 19.

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN.

STAT6 stain has proved to be a good surrogate marker for the genetic alteration (NAB2-STAT6 gene fusion) in solitary fibrous tumor (SFT). This study aims to validate the use of STAT6 rabbit monoclonal antibody in differentiating SFT from its histologic mimics. Forty-five cases of SFT and 110 cases from 9 other spindle cell tumors were collected for STAT6 immunostaining. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000745DOI Listing
January 2019
5 Reads

Annexin A10 and HES-1 Immunohistochemistry in Right-sided Traditional Serrated Adenomas Suggests an Origin From Sessile Serrated Adenoma.

Appl Immunohistochem Mol Morphol 2019 Jan 16. Epub 2019 Jan 16.

Department of Pathology and Laboratory Medicine, University of Calgary, Cumming School of Medicine and Calgary Laboratory Services, Calgary, AB, Canada.

There is increasing body of evidence to suggest that some colonic serrated polyps do not fit morphologically with any of the proposed categories for serrated polyps recommended by the World Health Organization. Most of these polyps have morphologic features of traditional serrated adenoma (TSA) admixed with areas resembling sessile serrated adenoma (SSA) or hyperplastic polyp (HP). Based on these findings it has been suggested that at least some TSAs may arise in association with precursor HP or SSA lesions, particularly those that develop in right colon. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000740DOI Listing
January 2019

NordiQC Assessments of Chromogranin A Immunoassays.

Appl Immunohistochem Mol Morphol 2019 Apr;27(4):258-262

Department of Medicine, Institute of Pathology, Aalborg University Hospital, and Aalborg University.

This paper is number 6 in a series developed through a partnership between ISIMM and Nordic IHC Quality Control with the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency testing program. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000743DOI Listing

Transferred-tissue Microarray for Fluorescence In Situ Hybridization Test for Human Epidermal Growth Factor Receptor 2 in Breast Cancer.

Appl Immunohistochem Mol Morphol 2019 Jan 16. Epub 2019 Jan 16.

Department of Pathology, Kaohsiung Medical University Hospital.

Context: Human epidermal growth factor receptor 2 (HER2) status of breast carcinomas is usually determined by immunohistochemical (IHC) staining and, if the IHC results are equivocal, in situ hybridization (ISH). Multiple ISH tests are sometimes required for multiple primary or metastatic tumors. A method for multiplex ISH test on tissues from multiple blocks is helpful in these situations. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000742DOI Listing
January 2019
8 Reads

Granuloma With an Underlying Lymphoma: A Diagnostic Challenge and a Wider Histologic Spectrum Including Adult T-Cell Leukemia/Lymphoma.

Appl Immunohistochem Mol Morphol 2019 Jan 16. Epub 2019 Jan 16.

Department of Pathology, School of Medicine, College of Medicine, Taipei Medical University, Taipei.

Granulomatous reaction is not uncommon in histopathology, with various etiologies in different organs and geographic regions. Lymphoma is one of the underlying causes of granuloma; and sometimes the neoplastic cells may be masked by the granulomatous reaction. In this report, we present our experience with 7 lymphoma cases of various histologic types with coexisting granuloma to show the diagnostic challenges. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000731DOI Listing
January 2019
2 Reads

BAP1 Loss is a Useful Adjunct to Distinguish Malignant Mesothelioma Including the Adenomatoid-like Variant From Benign Adenomatoid Tumors.

Appl Immunohistochem Mol Morphol 2019 Jan 11. Epub 2019 Jan 11.

Institute of Pathology, Comprehensive Cancer Center Erlangen-EMN, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen.

Malignant mesothelioma (MM) can show areas closely mimicking reactive mesothelial proliferations or recapitulating benign adenomatoid tumors (ATs) making distinction on occasion impossible on morphologic ground alone, particularly in limited biopsy material. Recently, loss of BAP1 by immunohistochemistry (IHC) has been suggested as a potential marker for identifying MM, but data is still limited. We studied 264 MM cases (257 using tissue microarrays; 7 on conventional slides) and 42 genital ATs for BAP1 immunohistochemical expression. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000700DOI Listing
January 2019
2 Reads

A Multi-Institutional Study to Evaluate Automated Whole Slide Scoring of Immunohistochemistry for Assessment of Programmed Death-Ligand 1 (PD-L1) Expression in Non-Small Cell Lung Cancer.

Appl Immunohistochem Mol Morphol 2019 Apr;27(4):263-269

Department of Pathology, Yale University School of Medicine, New Haven, CT.

Assessment of programmed death-ligand 1 (PD-L1) expression is a critical part of patient management for immunotherapy. However, studies have shown that pathologist-based analysis lacks reproducibility, especially for immune cell expression. The purpose of this study was to validate reproducibility of the automated machine-based Optra image analysis for PD-L1 immunohistochemistry for both tumor cells (TCs) and immune cells. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000737DOI Listing
April 2019
8 Reads

Myeloid Cell Nuclear Differentiation Antigen (MNDA) Positivity in Primary Follicles: Potential Pitfall in the Differential Diagnosis With Marginal Zone Lymphoma.

Appl Immunohistochem Mol Morphol 2019 Jan 11. Epub 2019 Jan 11.

Department of Pathology, Stanford University School of Medicine, Stanford, CA.

Myeloid cell nuclear differentiation antigen (MNDA) is an immunohistochemical marker that is used to distinguish marginal zone lymphomas (MZLs) from other small B-cell lymphomas. An index case that showed MNDA staining in primary follicles prompted the current study to evaluate whether MNDA expression is widespread in primary follicles and to address whether it poses a potential diagnostic pitfall. Of the 15 cases with primary follicles identified by a search of the laboratory information system, 7 had positive MNDA staining. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000738DOI Listing
January 2019

Immunohistochemistry Use by Diagnostic Category and Pathologist in 4477 Prostate Core Biopsy Sets Assessed at Two Hospitals.

Appl Immunohistochem Mol Morphol 2019 Jan 8. Epub 2019 Jan 8.

Surgery, Division of Urology, McMaster University.

Background: Immunohistochemistry (IHC) use in prostate cores is not routinely determined and its value assessed.

Methods: Pathology reports for cases accessioned 2011 to 2017 at two hospitals were retrieved. IHC orders by pathologist and hospital were extracted with a custom program and tabulated. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000726DOI Listing
January 2019
5 Reads

PTEN Loss in a Prostate Cancer Cohort From Jordan.

Appl Immunohistochem Mol Morphol 2019 Jan 4. Epub 2019 Jan 4.

Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, AB, Canada.

Deletion of phosphatase and tensin homolog (PTEN) in prostate cancer has been associated with early biochemical recurrence, increased metastatic potential, and androgen independence. We evaluated the status of PTEN loss in a cohort of prostate cancer patients from Jordan. We investigated 71 patients with prostate cancer and 52 control subjects with benign prostatic hyperplasia (BPH). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000732DOI Listing
January 2019

Epidermal Growth Factor Receptor Gene Mutation Status in Primary Lung Adenocarcinoma and Corresponding Bone Metastases.

Appl Immunohistochem Mol Morphol 2018 Dec 27. Epub 2018 Dec 27.

Department of Pathology, the First Affiliated Hospital and the College of Basic Medical Sciences of China Medical University, Shenyang, PR China.

Background: The aim of this study was to compare epidermal growth factor receptor (EGFR) mutations between primary tumors and corresponding bone metastases (BMs) in lung adenocarcinoma.

Materials And Methods: In total, 115 paired primary lung adenocarcinoma and corresponding BM tumors were analyzed for EGFR mutations by Amplification Refractory Mutation System.

Results: EGFR mutations were detected in 61 primary lung adenocarcinomas (53. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000725DOI Listing
December 2018
2 Reads

S100 Staining Adds to the Prognostic Significance of the Combination of Perineural Invasion and Lymphovascular Invasion in Colorectal Cancer.

Appl Immunohistochem Mol Morphol 2018 Dec 21. Epub 2018 Dec 21.

Departments of Medicine.

Studies have suggested that perineural invasion (PNI) and lymphovascular invasion (LVI) serve as independent prognostic factors in colorectal cancer (CRC). Currently, little is known regarding the combination of PNI and LVI as prognostic factors, independent of stage. We hypothesized that this combination was a better prognostic marker than either PNI or LVI alone, and that S100 staining would detect PNI not seen with hematoxylin and eosin (H&E). Read More

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http://dx.doi.org/10.1097/PAI.0000000000000733DOI Listing
December 2018

AQP1 and AQP3 Expression are Associated With Severe Symptoms and Poor-prognosis of the Pancreatic Ductal Adenocarcinoma.

Appl Immunohistochem Mol Morphol 2019 Jan;27(1):40-47

Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Approximately 80% of patients with pancreatic ductal adenocarcinoma (PDAC) have metastatic disease with poor prognosis, but clinically available biomarkers for the diagnosis, prediction of prognosis, and target therapy have not yet been identified.

Objective: To investigate the expression of aquaporin-1 (AQP1) and AQP3 protein and their clinicopathological significances in PDACs.

Materials And Method: AQP1 and AQP3 protein expression in 106 PDAC, 35 peritumoral tissues, 55 benign pancreatic lesions, and 13 normal pancreatic tissues was measured by immunohistochemistry. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000523DOI Listing
January 2019
1 Read

Immunoreactivity for Sox10 in Basaloid Neoplasms of The Skin.

Appl Immunohistochem Mol Morphol 2019 Feb;27(2):114-118

Section of Dermatopathology, Division of Surgical Pathology-Cytopathology, University of Virginia Medical Center, Charlottesville, VA.

Basaloid tumors of the skin pose a diagnostic challenge to pathologists, because the differential diagnosis is broad, sometimes with subtle differentiating features. We evaluated SOX10 expression in 120 primary cutaneous tumors with epidermal, sweat glandular, neuroendocrine/neuroectodermal, follicular, and sebaceous lineages. Our findings were compared with those of previous studies that evaluated SOX10 in tumors of the skin. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000721DOI Listing
February 2019
3 Reads

p53-Family Proteins in Odontogenic Cysts: An Immunohistochemical Study.

Appl Immunohistochem Mol Morphol 2018 Dec 4. Epub 2018 Dec 4.

Department of Clinical Specialistic and Dental Sciences.

The present study investigated the immunohistochemical expression of p53, p63, and p73 in different types of odontogenic cysts (OC), a group of common intraosseous jaw lesions, to provide a better understanding of p53-family functions in odontogenic lesions. We carried out immunohistochemical analysis to evaluate the expression of p53, p63, and p73 in 60 samples of OC, including dentigerous cysts, radicular cysts, orthokeratinized OC, and odontogenic keratocysts (OKC). The epithelial expression of p53-family members was evaluated both in the basal-parabasal and in the superficial layers, measuring the percentage of positive cells and the value of expression intensity. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000727DOI Listing
December 2018
3 Reads

On the Concept of Tumor Homogeneity.

Authors:
Louis Libbrecht

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology, University Hospital Saint-Luc, Brussels, Belgium.

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http://dx.doi.org/10.1097/PAI.0000000000000719DOI Listing
November 2018
1 Read

NordiQC Assessments of CD117 Immunoassays.

Appl Immunohistochem Mol Morphol 2019 Feb;27(2):87-91

Institute of Pathology, Aalborg University Hospital.

This paper is the number 5 in a series developed through a partnership between ISIMM and NordiQC for the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency-testing program. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000714DOI Listing
February 2019
11 Reads

Application of Chromosome Microarray Analysis for the Differential Diagnosis of Low-grade Renal Cell Carcinoma With Clear Cell and Papillary Features.

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology.

Clear cell renal cell carcinoma (ccRCC) and papillary renal cell carcinoma (pRCC) are the 2 most common RCCs. However, some RCCs can have both clear cell and papillary features, including clear cell papillary RCC (ccpRCC). They can be a diagnostic challenge in daily practice. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000704DOI Listing
November 2018
1 Read

Utilization Rate of Helicobacter pylori Immunohistochemistry Is Not Associated With the Diagnostic Rate of Helicobacter pylori Infection.

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology and Cell Biology.

Background: Utilization rates of immunohistochemistry (IHC) for the diagnosis of Helicobacter pylori infection may vary by laboratory and/or pathologists. IHC for H. pylori is not performed routinely in our practice. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000680DOI Listing
November 2018
2 Reads

Combining ALK Fluorescent In Situ Hybridization and Immunohistochemistry to Analyze Multiple Non-Small Cell Lung Carcinoma Samples per Patient Reveals Intermethod and Intersample-discrepant Results.

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology, CHRU Brest, Brest.

ALK inhibitors have improved the therapeutic management of patients with ALK-rearranged advanced non-small cell lung cancers (NSCLC). Several diagnostic methods, mainly fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC), can be used as single or combined tests to detect the so-called "ALK-positive" NSCLC. Intersample and intermethod discrepancies could cause issues with therapeutic consequences in ALK testing. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000675DOI Listing
November 2018

Consecutive Immunohistochemical Staining on a Single Slide is Effective in Confirming Melanocytic Derivation.

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Departments of Pathology.

Background: Metastatic melanoma in sentinel lymph nodes is often elusive to detect with morphology alone. Per American Joint Committee on Cancer staging guidelines, a single atypical melanocyte in lymph node qualifies as metastasis, whether identified by morphology or immunohistochemistry, but single cell staining must be convincing. We propose that the use of a second immunohistochemical run performed on a single slide will allow for more confident diagnosis of micrometastases. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000665DOI Listing
November 2018
15 Reads

Programmed Death-ligand 1 (PD-L1) Expression in Thymic Epithelial Tumors.

Appl Immunohistochem Mol Morphol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Thymic epithelial tumors (TETs) are uncommon neoplasms of the mediastinum. The gold standard treatment is complete surgical resection which can be followed by radio/chemotherapy in selected cases. Targeted tyrosine kinase inhibition can be considered in only a limited number of aggressive or metastatic tumors as EGFR, BRAF, or c-kit mutations are rare. Read More

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http://dx.doi.org/10.1097/PAI.0000000000000699DOI Listing
November 2018
14 Reads