393,668 results match your criteria Apoptosis : an international journal on programmed cell death[Journal]


The toxic potentials and focus of disinfection byproducts based on the human embryonic kidney (HEK293) cell model.

Sci Total Environ 2019 Jan 29;664:948-957. Epub 2019 Jan 29.

Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Disinfection byproducts (DBPs) are inevitably generated during drinking water disinfection processes, and their hazards have not been well characterized. Because they plausibly cause toxicological and pathological damage to human kidney, we selected the human embryonic kidney (HEK293) cell, instead of the commonly used CHO cell, as a model to investigate the toxic potential and target of 10 DBPs, including 3 haloacetamides, 2 trihaloacetaldehydes and 5 iodomethanes. Based on the chronic toxicity parameter EC of the cell viability test, we obtained a toxic rank of the tested DBPs different from previous studies and calculated their risk quotients by combining their actual concentrations in drinking water systems. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.01.361DOI Listing
January 2019

Thioredoxin system as a gatekeeper in caspase-6 activation and nuclear lamina integrity: Implications for Alzheimer's disease.

Free Radic Biol Med 2019 Feb 12. Epub 2019 Feb 12.

Regenerative Medicine Program and Spinal Cord Research Centre, Canada; Dept. Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada. Electronic address:

Recent reports in pathophysiology of neurodegenerative diseases (ND) have linked nuclear lamina degradation/deficits to neuronal cell death. Lamin-B1 damage is specifically involved in this process leading to nuclear envelope invagination and heterochromatin rearrangement. The underlying mechanisms involved in these events are not yet defined. Read More

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http://dx.doi.org/10.1016/j.freeradbiomed.2019.02.010DOI Listing
February 2019

Lipoic Acid-derived Cross-linked Liposomes for Reduction-responsive Delivery of Anticancer Drug.

Int J Pharm 2019 Feb 12. Epub 2019 Feb 12.

School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China. Electronic address:

Liposomes have emerged as a fascinating nanocarriers for the delivery of cancer therapeutics. However, their efficacy for cancer therapy is reduced because of the serum-instability and incomplete drug release. In this study, a novel disulfide cross-linked liposomes (CLs) assembled from dimeric lipoic acid-derived glycerophosphorylcholine (di-LA-PC) conjugate was developed. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.02.007DOI Listing
February 2019

Edaravone reduces Aβ-induced oxidative damage in SH-SY5Y cells by activating the Nrf2/ARE signaling pathway.

Life Sci 2019 Feb 12. Epub 2019 Feb 12.

Department of Neurology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China. Electronic address:

Aims: Edaravone potentially alleviates cognitive deficits in a mouse model of Alzheimer's disease (AD). However, the mechanism of edaravone in suppressing AD progression remains unclear. We aim to investigate the mechanism of edaravone in suppressing oxidative stress-mediated AD progression in vitro. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.025DOI Listing
February 2019

Concurrent neprilysin inhibition and renin-angiotensin system modulations prevented diabetic nephropathy.

Life Sci 2019 Feb 12. Epub 2019 Feb 12.

Laboratory of Molecular Pharmacology, Department of Pharmacy, Birla Institute of Technology and Science Pilani, Pilani Campus, Rajasthan 333031, India. Electronic address:

Aims: Renin-angiotensin system (RAS) and natriuretic peptides system (NPS) perturbations govern the development of diabetic nephropathy (DN). Hence, in search of a novel therapy against DN, present study targeted both, NPS and RAS simultaneously using a neprilysin inhibitor (NEPi) in combination with either angiotensin receptor blocker (ARB) or angiotensin-converting enzyme 2 (ACE2) activator.

Methods: We induced diabetes in male Wistar rats by a single dose of streptozotocin (55 mg/kg, i. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.027DOI Listing
February 2019

PRSS23 knockdown inhibits gastric tumorigenesis through EIF2 signaling.

Pharmacol Res 2019 Feb 12. Epub 2019 Feb 12.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Key Laboratory of Digestive Diseases of Jiangxi Province, Nanchang, China. Electronic address:

PRSS23 is a newly discovered serine protease that has been associated with tumor progression in various types of cancers. Our previous study showed PRSS23 is down-regulated obviously in Hedgehog pathway blocked gastric cancer cells. However, the correlation between PRSS23 and tumor progression of gastric cancer remains unclear. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.008DOI Listing
February 2019

γ-Tocotrienol inhibits oxidative phosphorylation and triggers apoptosis by inhibiting mitochondrial complex I subunit NDUFB8 and complex II subunit SDHB.

Toxicology 2019 Feb 12. Epub 2019 Feb 12.

Department of Clinical Nutrition, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, China; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, 200030, China. Electronic address:

Tocotrienols (T3s) are a subgroup of vitamin E and they have been widely tested to inhibit cell growth in various tumor types. Previous studies have shown that T3s inhibit cancer cell growth by targeting multiple signaling transduction and cellular processes. However, the role of T3s in the regulation of cellular bioenergetic processes remains unclear. Read More

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http://dx.doi.org/10.1016/j.tox.2019.01.018DOI Listing
February 2019

The protective effects of the antioxidant N-acetylcysteine (NAC) against oxidative stress-associated apoptosis evoked by the organophosphorus insecticide malathion in normal human astrocytes.

Toxicology 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacy, Tajen University, Pingtung, 90741, Taiwan, ROC. Electronic address:

Malathion is one of the most widely used organophosphorus insecticides in agriculture. However, malathion may be involved in the etiology of human brain dysfunction. Induction of ROS has been proposed as a mechanism of malathion-induced poisoning cases, but there are few data regarding the effects of malathion on oxidative stress-associated neurotoxicity in human glial cells. Read More

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http://dx.doi.org/10.1016/j.tox.2019.02.004DOI Listing
February 2019

Mangiferin alleviates arsenic induced oxidative lung injury via upregulation of the Nrf2-HO1 axis.

Food Chem Toxicol 2019 Feb 12. Epub 2019 Feb 12.

Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, 700054, India. Electronic address:

Arsenic contaminated drinking water consumption is a serious health issue around the world. Chronic inorganic arsenic exposure has been associated with respiratory dysfunctions. It exerts various detrimental effects, disrupting normal cellular homeostasis and turning on severe pulmonary complications. Read More

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http://dx.doi.org/10.1016/j.fct.2019.02.022DOI Listing
February 2019

Stem extract of Tabebuia chrysantha induces apoptosis by targeting sEGFR in Ehrlich Ascites Carcinoma.

J Ethnopharmacol 2019 Feb 12. Epub 2019 Feb 12.

Pharmaceutical Analysis Laboratory, SIMS College of Pharmacy, Guntur, India. Electronic address:

Ethnopharmacological Relevance: The plant Tabebuia chrysantha (Jaq.) Nicholson (Bignoniaceae) is commonly known as "Golden Goddess" in the Southern part of India and "Golden Trumpet Tree " in Central America. Stems of this plant have been traditionally used for antioxidant, anti-inflammatory, antimicrobial and anticancer actions. Read More

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http://dx.doi.org/10.1016/j.jep.2019.02.023DOI Listing
February 2019

Forsythiaside prevents β-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-κB pathway.

Neurochem Int 2019 Feb 12. Epub 2019 Feb 12.

Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China. Electronic address:

In the study, the neuroprotectivities of forsythiaside, a main constituent of Forsythia suspensa (Thunb.) Vahl (F. suspensa, Lianqiao in Chinese), were investigated in the hippocampal slices. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.008DOI Listing
February 2019

Vorinostat synergizes with Antioxidant therapy to target Myeloproliferative Neoplasms.

Exp Hematol 2019 Feb 12. Epub 2019 Feb 12.

Unidade de Investigação em Patobiologia Molecular, Instituto Português de Oncologia de Lisboa Francisco Gentil, E.P.E, Lisboa, Portugal; Centro de Estudos de Doenças Crónicas, CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisboa, Portugal; Hospital da Luz, Lisboa, Portugal. Electronic address:

The BCR-ABL-negative myeloproliferative neoplasms (MPN) are driven by JAK-STAT pathway activation but epigenetic alterations also play an important pathophysiological role. These can be pharmacologically manipulated with histone deacetylase inhibitors (HDACis), which have proven to be clinically effective in the treatment of MPN but exhibiting dose-limiting toxicities. The treatment of primary MPN cells with Vorinostat modulates the expression of genes associated with apoptosis, cell cycle, inflammation and signalling. Read More

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http://dx.doi.org/10.1016/j.exphem.2019.02.002DOI Listing
February 2019

Effects of norfloxacin exposure on neurodevelopment of zebrafish (Danio rerio) embryos.

Neurotoxicology 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 185 Donghu Road, Wuhan, Hubei 430071, China. Electronic address:

In view of the wide application of fluoroquinolones (FQs), a group of broad-spectrum synthetic antibacterial agents, and their large ingress into the environment, the toxic effects on non-target organisms caused by FQs have received great attention. In this study, we used zebrafish embryo as a model, measured the general toxic effects of norfloxacin, a commonly used FQs, and investigated the effects of norfloxacin on the neurodevelopment of zebrafish embryos. Our data showed that norfloxacin significantly inhibited the hatching rate of zebrafish embryos, and increased the mortality and malformation rate of the embryos. Read More

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http://dx.doi.org/10.1016/j.neuro.2019.02.007DOI Listing
February 2019

Identification of microRNA-mRNA networks involved in cisplatin-induced renal tubular epithelial cells injury.

Eur J Pharmacol 2019 Feb 12. Epub 2019 Feb 12.

Department of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, 100730, China. Electronic address:

Cisplatin is a widely used chemotherapeutic drug that often causes acute kidney injury (AKI) in cancer patients. The contribution of miRNAs to the cisplatin-induced renal tubular epithelial cell injury remains largely unknown. Here we performed an integrative network analysis of miRNA and mRNA expression profiles to shed light into the underlying mechanism of cisplatin-induced renal tubular epithelial cell injury. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.02.015DOI Listing
February 2019

Anti-tumor activity of antibody drug conjugate targeting aspartate-β-hydroxylase in pancreatic ductal adenocarcinoma.

Cancer Lett 2019 Feb 12. Epub 2019 Feb 12.

Liver Research Center, Rhode Island Hospital, Warren Alpert Medical School, Brown University, Providence, Rhode Island. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive malignancy with very limited treatment options. Antibody drug conjugates (ADCs) are promising cytotoxic agents capable of highly selective delivery. Aspartate-β-hydroxylase (ASPH) is a type II transmembrane protein highly expressed in PDACs (97. Read More

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http://dx.doi.org/10.1016/j.canlet.2019.02.006DOI Listing
February 2019

Cytoophidia respond to nutrient stress in Drosophila.

Exp Cell Res 2019 Feb 12. Epub 2019 Feb 12.

School of Life Science and Technology, ShanghaiTech University, 230 Haike Road, 201210, Shanghai, China; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3PT, United Kingdom. Electronic address:

CTP synthase (CTPsyn) is a metabolic enzyme essential for the de novo synthesis of CTP the nucleotide. CTPsyn can be compartmented into filamentous structures named cytoophidia. Cytoophidia are conserved in a wide range of species and are highly abundant in Drosophila ovaries. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.003DOI Listing
February 2019

Long Non-Coding RNA TFAP2A-AS1 Inhibits Cell Proliferation and Invasion in Breast Cancer via miR-933/SMAD2.

Med Sci Monit 2019 Feb 15;25:1242-1253. Epub 2019 Feb 15.

Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).

BACKGROUND It is well documented that long non-coding RNAs (lncRNAs) are involved in the progression of multiple human tumors by sponging microRNAs (miRNAs). However, whether lncRNA TFAP2A-AS1 plays a role in the tumorigenesis of breast cancer (BC) remains undetermined. MATERIAL AND METHODS Real-time PCR (qRT-PCR) assay was performed to detect the relative mRNA expression of TFAP2A-AS1 and miR-933. Read More

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http://dx.doi.org/10.12659/MSM.912421DOI Listing
February 2019

Dexamethasone during pregnancy impairs maternal pancreatic β-cell renewal during lactation.

Endocr Connect 2019 Feb;8(2):120-131

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.

Pancreatic islets from pregnant rats develop a transitory increase in the pancreatic β-cell proliferation rate and mass. Increased apoptosis during early lactation contributes to the rapid reversal of those morphological changes. Exposure to synthetic glucocorticoids during pregnancy has been previously reported to impair insulin secretion, but its impacts on pancreatic islet morphological changes during pregnancy and lactation have not been described. Read More

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http://dx.doi.org/10.1530/EC-18-0505DOI Listing
February 2019

RCC2, a regulator of the RalA signaling pathway, is identified as a novel therapeutic target in cisplatin-resistant ovarian cancer.

FASEB J 2019 Feb 15:fj201801529RR. Epub 2019 Feb 15.

Department of Gynecology, People's Hospital of Huadu District, Guangzhou, China.

Currently, cisplatin (DDP) is the first-line chemotherapeutic agent used for treatment of ovarian cancer, but gradually acquired drug resistance minimizes its therapeutic outcomes. We aimed to identify crucial genes associated with DDP resistance in ovarian cancer and uncover potential mechanisms. Two sets of gene expression data were downloaded from Gene Expression Omnibus, and bioinformatics analysis was conducted. Read More

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http://dx.doi.org/10.1096/fj.201801529RRDOI Listing
February 2019

Convenient access to 5-membered cyclic iminium ions: evidence for a stepwise [4 + 2] cycloaddition mechanism.

Org Biomol Chem 2019 Feb 15. Epub 2019 Feb 15.

School of Chemical Sciences, The University of Auckland, Symonds Street, Auckland 1010, New Zealand. and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Symonds Street, Auckland 1010, New Zealand.

In situ generation and reaction of novel 5-membered N-tosyl cyclic α,β-unsaturated iminium ions from readily prepared stable precursors is demonstrated. Formal iminium Diels-Alder cycloaddition proceeded in good yield via a stepwise rather than concerted cycloaddition process, confirmed through the isolation of a Mukaiyama-Michael type intermediate. Relative stereochemistry was determined upon subsequent intramolecular cyclisation under Lewis acid catalysis to afford formal endo 5,6-spirobicyclic adducts, as confirmed by crystallography. Read More

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http://dx.doi.org/10.1039/c9ob00262fDOI Listing
February 2019

IL-35 prevent bone loss through promotion of bone formation and angiogenesis in rheumatoid arthritis.

Clin Exp Rheumatol 2019 Feb 11. Epub 2019 Feb 11.

Department of Rheumatology and Immunology, the First Affiliated Hospital of China Medical University, Shenyang, China.

Objectives: Angiogenesis in bone and osteogenesis appear to be closely linked, suggesting the existence of molecular crosstalk between pro-angiogenic molecules and osteoblasts. The pro-angiogenic molecules vascular endothelial growth factor (VEGF) with its receptors Flt-1, Flk-1 and fibroblast growth factor (FGF)-2 play a crucial role in born formation, an early and critical event in the pathogenesis of rheumatoid arthritis (RA). Interleukin (IL)-35 is demonstrated to be an anti-inflammatory cytokine in RA. Read More

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February 2019

Group A -Mediated Host Cell Signaling.

Authors:
Vijay Pancholi

Microbiol Spectr 2019 Jan;7(1)

Department of Pathology, The Ohio State University College of Medicine, Columbus, OH 43210.

In the past decade, the field of the cellular microbiology of group A () infection has made tremendous advances and touched upon several important aspects of pathogenesis, including receptor biology, invasive and evasive phenomena, inflammasome activation, strain-specific autophagic bacterial killing, and virulence factor-mediated programmed cell death. The noteworthy aspect of -mediated cell signaling is the recognition of the role of M protein in a variety of signaling events, starting with the targeting of specific receptors on the cell surface and on through the induction and evasion of NETosis, inflammasome, and autophagy/xenophagy to pyroptosis and apoptosis. Variations in reports on -mediated signaling events highlight the complex mechanism of pathogenesis and underscore the importance of the host cell and strain specificity, as well as / experimental parameters. Read More

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http://dx.doi.org/10.1128/microbiolspec.GPP3-0021-2018DOI Listing
January 2019

Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes.

Zygote 2019 Feb 15:1-6. Epub 2019 Feb 15.

1Laboratory of Physiology and Biotechnology of Animal Reproduction,Federal University of San Francisco Valley,Petrolina,Brazil.

SummaryThe present study aimed to investigate the effect of quercetin as an alternative antioxidant to cysteamine on in vitro maturation. Oocytes were collected from goat ovaries, destined for in vitro maturation and distributed into three groups: CIS group, oocytes were immersed in MIV base medium; in Groups Q4 and Q8, oocytes were immersed in the medium of the CIS group, adding 4 μM or 8 μM of quercetin, respectively, and cultured for 24 h at 38.5°C with 5% CO2. Read More

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http://dx.doi.org/10.1017/S0967199418000485DOI Listing
February 2019

Tumor necrosis factor-related apoptosis inducing ligand induces apoptosis in canine hemangiosarcoma cells in vitro.

Vet Comp Oncol 2019 Feb 15. Epub 2019 Feb 15.

Laboratory of Veterinary Pathology, Gifu University, Gifu, Japan.

Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is an apoptosis-inducing cytokine that shows potential therapeutic value for human neoplasms, and is effective in some canine tumors; however, its potential for killing canine hemangiosarcoma (HSA) cells is unknown. Thus, we evaluated the proapoptotic effect of TRAIL in nine canine HSA cell lines. Cells (JuA1, JuB2, JuB2-1, JuB4, Re11, Re12, Re21, Ud2, and Ud6) were cultured with three recombinant human TRAILs (rhTRAILs): TRAIL-TEC derived from Escherichia coli, TRAIL-TL derived from mammalian cells, and izTRAIL containing an isoleucine-zippered structure that facilitates trimerization. Read More

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http://dx.doi.org/10.1111/vco.12471DOI Listing
February 2019

The selective cyclooxygenase-2 inhibitor mavacoxib (Trocoxcil™) exerts anti-tumour effects in-vitro independent of cyclooxygenase-2 expression levels.

Vet Comp Oncol 2019 Feb 15. Epub 2019 Feb 15.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian.

The inducible inflammatory enzyme cyclooxygenase-2 (COX-2) and its product prostaglandin E2 (PGE ) are prominent tumour promoters, and expression of COX-2 is elevated in a number of tumours of both humans and canines. Targeting COX-2 in cancer is an attractive option due to readily available non-steroidal anti-inflammatory drugs (NSAIDs), and there is a clear epidemiological link between NSAID use and cancer risk. In this study we aim to establish the anti-tumourigenic effects of the selective, long-acting COX-2 inhibitor mavacoxib. Read More

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http://dx.doi.org/10.1111/vco.12470DOI Listing
February 2019

Upregulation of long noncoding RNA SNHG20 promotes cell growth and metastasis in esophageal squamous cell carcinoma via modulating ATM-JAK-PD-L1 pathway.

J Cell Biochem 2019 Feb 14. Epub 2019 Feb 14.

Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu, China.

Increasing evidence have proved that long noncoding RNAs (lncRNAs) play significant roles in tumorigenesis and development of various cancers. However, the effect of small nucleolar RNA host gene 20 (SNHG20) on the progression of esophageal squamous cell carcinoma (ESCC) remains to be discovered. Herein, we aim to find out the function and the possible mechanism of SNHG20 in ESCC progression. Read More

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http://dx.doi.org/10.1002/jcb.28444DOI Listing
February 2019

MFGE8 protects against CCl -induced liver injury by reducing apoptosis and promoting proliferation of hepatocytes.

J Cell Physiol 2019 Feb 14. Epub 2019 Feb 14.

Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, China.

Milk fat globule-EGF factor 8 (MFGE8) has been reported to play various roles in acute injury and inflammation response. However, the role of MFGE8 in liver injury is poorly investigated. The present research was designed to clarify the expression and function of MFGE8 in carbon tetrachloride (CCl )-induced liver injury. Read More

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http://dx.doi.org/10.1002/jcp.28314DOI Listing
February 2019

Modafinil protects hippocampal neurons by suppressing excessive autophagy and apoptosis in mice with sleep deprivation.

Br J Pharmacol 2019 Feb 14. Epub 2019 Feb 14.

Shanghai Key Laboratory of Compound Chinese Medicines, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China.

Background And Purpose: Sleep deprivation (SD) compromises learning and memory in both humans and animals, which can be reversed by administration of modafinil, a wake-promoting drug. Dysfunctional autophagy promotes the activation of apoptotic cascades, ultimately leading to an increased neuronal death, which can be alleviated by autophagy inhibitors. This study is aimed to investigate the alleviative effect and mechanism of modafinil on the excessive autophagy in hippocampus of mice with deficiency of learning and memory induced by SD. Read More

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http://dx.doi.org/10.1111/bph.14626DOI Listing
February 2019

Protection of miR-19b in hypoxia/reoxygenation-induced injury by targeting PTEN.

J Cell Physiol 2019 Feb 14. Epub 2019 Feb 14.

Department of Neurology, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Objective: To study the role and mechanism of microRNA 19b (miR-19b) in hypoxia/reoxygenation (H/R)-induced injury by targeting PTEN.

Methods: PC12 and BV2 cells induced by H/R were treated with miR-19b mimics/inhibitors or small interfering PTEN (si-PTEN), respectively. Lactate dehydrogenase (LDH) level, malondialdehyde (MDA), and superoxide dismutase (SOD) content was detected. Read More

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http://dx.doi.org/10.1002/jcp.28286DOI Listing
February 2019

Autophagy inhibition with chloroquine reverts paclitaxel resistance and attenuates metastatic potential in human nonsmall lung adenocarcinoma A549 cells via ROS mediated modulation of β-catenin pathway.

Apoptosis 2019 Feb 14. Epub 2019 Feb 14.

Department of Biotechnology and Dr. B. C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Ballygunge, Kolkata, West Bengal, 700 019, India.

Paclitaxel is one of the most commonly used drugs for the treatment of nonsmall cell lung cancer (NSCLC). However acquired resistance to paclitaxel, epithelial to mesenchymal transition and cancer stem cell formation are the major obstacles for successful chemotherapy with this drug. Some of the major reasons behind chemoresistance development include increased ability of the cancer cells to survive under stress conditions by autophagy, increased expression of drug efflux pumps, tubulin mutations etc. Read More

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http://dx.doi.org/10.1007/s10495-019-01526-yDOI Listing
February 2019

The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent.

Pflugers Arch 2019 Feb 15. Epub 2019 Feb 15.

Department of Clinical Science, SUS, Division of Islet Cell Physiology, University of Lund, Jan Waldenströmsgata 35, Building 91, Floor 11, SE-205 02, Malmö, Sweden.

We have recently shown that the G protein-coupled receptor 142 (GPR142) is expressed in both rodent and human pancreatic β-cells. Herein, we investigated the cellular distribution of GPR142 within islets and the effects of selective agonists of GPR142 on glucose-stimulated insulin secretion (GSIS) in the mouse islets and INS-1832/13 cells. Double-immunostaining revealed that GPR142 immunoreactivity in islets mainly occurs in insulin-positive cells. Read More

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http://dx.doi.org/10.1007/s00424-019-02262-7DOI Listing
February 2019

An Inhibitor of Arginine-Glycine-Aspartate-Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis.

Hepatol Commun 2019 Feb 27;3(2):246-261. Epub 2018 Dec 27.

Division of Gastroenterology and Hepatology Saint Louis University St. Louis MO.

The presence and stage of liver fibrosis in patients with nonalcoholic steatohepatitis (NASH) is strongly associated with mortality. Thus, both preventing and reversing fibrosis are critically important approaches to prevent death or the need for liver transplantation from NASH. Recently, fibrosis in several mouse models of organ injury was shown to be prevented and reversed with the potent small molecule, arginine-glycine-aspartic acid tripeptide (RGD)-binding, integrin antagonist (3S)-3-(3-bromo-5-(tert-butyl)phenyl)-3-(2-(3-hydroxy-5-((5-hydroxy-1,4,5,6-tetrahydropyrimidin-2-yl)amino)benzamido)acetamido)propanoic acid (Center for World Health and Medicine [CWHM]-12). Read More

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http://dx.doi.org/10.1002/hep4.1298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357833PMC
February 2019

Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines .

Biomed Res Int 2019 15;2019:6146972. Epub 2019 Jan 15.

Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, SA, 5052, Australia.

The combination of doxorubicin and cyclophosphamide commonly used to treat breast cancer can cause premature ovarian failure and infertility. -Tocopherol is a potent antioxidant whereas -tocopherol causes apoptosis in a variety of cancer models including breast cancer. We hypothesised that the combination of doxorubicin (Dox) and 4-hydroperoxycyclophosphamide (4-Cyc) would be more cytotoxic than each agent alone, and that -tocopherol would reduce and -tocopherol would augment the cytotoxicity of the combined chemotherapeutics. Read More

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http://dx.doi.org/10.1155/2019/6146972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350544PMC
January 2019

A Novel Small Molecule p53 Stabilizer for Brain Cell Differentiation.

Front Chem 2019 31;7:15. Epub 2019 Jan 31.

Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal.

Brain tumor, as any type of cancer, is assumed to be sustained by a small subpopulation of stem-like cells with distinctive properties that allow them to survive conventional therapies and drive tumor recurrence. Thus, the identification of new molecules capable of controlling stemness properties may be key in developing effective therapeutic strategies for cancer by inducing stem-like cells differentiation. Spiropyrazoline oxindoles have previously been shown to induce apoptosis and cell cycle arrest, as well as upregulate p53 steady-state levels, while decreasing its main inhibitor MDM2 in the HCT116 human colorectal carcinoma cell line. Read More

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http://dx.doi.org/10.3389/fchem.2019.00015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365904PMC
January 2019

Exploration of the chondrosarcoma metabolome; the mTOR pathway as an important pro-survival pathway.

J Bone Oncol 2019 Apr 29;15:100222. Epub 2019 Jan 29.

Department of Pathology, Leiden University Medical Centre, Leiden, the Netherlands.

Background: Chondrosarcomas are malignant cartilage-producing tumors showing mutations and changes in gene expression in metabolism related genes. In this study, we aimed to explore the metabolome and identify targetable metabolic vulnerabilities in chondrosarcoma.

Methods: A custom-designed metabolic compound screen containing 39 compounds targeting different metabolic pathways was performed in chondrosarcoma cell lines JJ012, SW1353 and CH2879. Read More

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http://dx.doi.org/10.1016/j.jbo.2019.100222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360255PMC

Novel fluorescent probes of 10-hydroxyevodiamine: autophagy and apoptosis-inducing anticancer mechanisms.

Acta Pharm Sin B 2019 Jan 20;9(1):144-156. Epub 2018 Aug 20.

School of Pharmacy, Second Military Medical University, Shanghai 200433, China.

Natural product evodiamine and its derivatives represent a promising class of multi-target antitumor agents. However, the clinical development of these compounds has been hampered by a poor understanding of their antitumor mechanisms. To tackle this obstacle, herein, novel fluorescent probes were designed to elucidate the antitumor mode of action of 10-hydroxyevodiamine. Read More

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http://dx.doi.org/10.1016/j.apsb.2018.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361730PMC
January 2019

Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway.

Cancer Biol Med 2018 Nov;15(4):400-414

State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.

Objective: Small cell lung carcinoma (SCLC) is considered one of the most aggressive types of lung cancer due to its rapid growth and early metastasis. No tumor markers or therapeutic targets have been demonstrated to be specific or effective in SCLC to date. This study aims to evaluate the potential of Flotillin1 (Flot1) as a target of SCLC treatment. Read More

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http://dx.doi.org/10.20892/j.issn.2095-3941.2018.0053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372910PMC
November 2018

Targeting the BIR Domains of Inhibitor of Apoptosis (IAP) Proteins in Cancer Treatment.

Comput Struct Biotechnol J 2019 25;17:142-150. Epub 2019 Jan 25.

Department of Research, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo 42, 20133 Milan, Italy.

Inhibitor of apoptosis (IAP) proteins are characterized by the presence of the conserved baculoviral IAP repeat (BIR) domain that is involved in protein-protein interactions. IAPs were initially thought to be mainly responsible for caspase inhibition, acting as negative regulators of apoptosis, but later works have shown that IAPs also control a plethora of other different cellular pathways. As X-linked IAP (XIAP), and other IAP, levels are often deregulated in cancer cells and have been shown to correlate with patients' prognosis, several approaches have been pursued to inhibit their activity in order to restore apoptosis. Read More

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http://dx.doi.org/10.1016/j.csbj.2019.01.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360406PMC
January 2019

Zinc Oxide Nanoparticles Exhibit Both Cyclooxygenase- and Lipoxygenase-Mediated Apoptosis in Human Bone Marrow-Derived Mesenchymal Stem Cells.

Toxicol Res 2019 Jan 15;35(1):83-91. Epub 2018 Jan 15.

Department of Veterinary Toxicology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea.

Nanoparticles (NPs) have been recognized as both useful tools and potentially toxic materials in various industrial and medicinal fields. Previously, we found that zinc oxide (ZnO) NPs that are neurotoxic to human dopaminergic neuroblastoma SH-SY5Y cells are mediated by lipoxygenase (LOX), not cyclooxygenase-2 (COX-2). Here, we examined whether human bone marrow-derived mesenchymal stem cells (MSCs), which are different from neuroblastoma cells, might exhibit COX-2- and/or LOX-dependent cytotoxicity of ZnO NPs. Read More

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http://dx.doi.org/10.5487/TR.2019.35.1.083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354944PMC
January 2019

Corrigendum: Seabuckthorn Pulp Oil Protects against Myocardial Ischemia-Reperfusion Injury in Rats through Activation of Akt/eNOS.

Front Pharmacol 2018 31;9:1557. Epub 2019 Jan 31.

Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Abu Dhabi, UAE.

[This corrects the article DOI: 10.3389/fphar.2016. Read More

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http://dx.doi.org/10.3389/fphar.2018.01557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365674PMC
January 2019

Corrigendum: HAX-1 Protects Glioblastoma Cells From Apoptosis Through the Akt1 Pathway.

Front Cell Neurosci 2019 31;13:13. Epub 2019 Jan 31.

Department of Neuro-interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

[This corrects the article DOI: 10.3389/fncel.2017. Read More

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http://dx.doi.org/10.3389/fncel.2019.00013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366153PMC
January 2019

Epigenetic drugs and their molecular targets in testicular germ cell tumours.

Nat Rev Urol 2019 Feb 14. Epub 2019 Feb 14.

Institute of Pathology, Department of Developmental Pathology, University of Bonn Medical School, Bonn, Germany.

Current treatment regimens for type II testicular germ cell tumours (TGCTs) achieve cure rates of ≥95%; however, 1-5% of TGCTs develop resistance to standard platinum-based chemotherapy. Patients with recurrent TGCT typically receive high-dose chemotherapy, but this treatment results in severe adverse effects and cytotoxicity. Thus, alternative treatment options should be considered to improve patient well-being and quality of life. Read More

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http://dx.doi.org/10.1038/s41585-019-0154-xDOI Listing
February 2019

TYRO3 as a molecular target for growth inhibition and apoptosis induction in bladder cancer.

Br J Cancer 2019 Feb 15. Epub 2019 Feb 15.

Institut Curie, PSL Research University, CNRS, UMR144, Equipe Labellisée Ligue contre le Cancer, 75005, Paris, France.

Background: Muscle-invasive bladder cancer (MIBC) is an aggressive neoplasm with poor prognosis, lacking effective therapeutic targets. Oncogenic dependency on members of the TAM tyrosine kinase receptor family (TYRO3, AXL, MERTK) has been reported in several cancer types, but their role in bladder cancer has never been explored.

Methods: TAM receptor expression was evaluated in two series of human bladder tumours by gene expression (TCGA and CIT series), immunohistochemistry and western blotting analyses (CIT series). Read More

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http://dx.doi.org/10.1038/s41416-019-0397-6DOI Listing
February 2019

Inhibition of G9a by a small molecule inhibitor, UNC0642, induces apoptosis of human bladder cancer cells.

Acta Pharmacol Sin 2019 Feb 14. Epub 2019 Feb 14.

Department of Urology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, 210008, China.

Urinary bladder cancer (UBC) is characterized by frequent recurrence and metastasis despite the standard chemotherapy with gemcitabine and cisplatin combination. Histone modifiers are often dysregulated in cancer development, thus they can serve as an excellent drug targets for cancer therapy. Here, we investigated whether G9a, one of the histone H3 methyltransferases, was associated with UBC development. Read More

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http://dx.doi.org/10.1038/s41401-018-0205-5DOI Listing
February 2019

Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida.

Sci Rep 2019 Feb 14;9(1):2101. Epub 2019 Feb 14.

Dalian Ocean University, Dalian, P. R. China.

Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. Read More

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http://dx.doi.org/10.1038/s41598-019-38890-3DOI Listing
February 2019

Rosiglitazone blocks first trimester in-vitro placental injury caused by NF-κB-mediated inflammation.

Sci Rep 2019 Feb 14;9(1):2018. Epub 2019 Feb 14.

Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, Grand Rapids, MI, USA.

Increased inflammation and abnormal placentation are common features of a wide spectrum of pregnancy-related disorders such as intra uterine growth restriction, preeclampsia and preterm birth. The inflammatory response of the human placenta has been mostly investigated in relation to cytokine release, but the direct molecular consequences on trophoblast differentiation have not been investigated. This study measured the general effects of LPS on both extravillous and villous trophoblast physiology, and the involvement of the transcription factors PPARγ and NF-κB, specifically using 1 trimester explants and HTR-8/ SVneo cell line models. Read More

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http://dx.doi.org/10.1038/s41598-018-38336-2DOI Listing
February 2019

miR-302a inhibits human HepG2 and SMMC-7721 cells proliferation and promotes apoptosis by targeting MAP3K2 and PBX3.

Sci Rep 2019 Feb 14;9(1):2032. Epub 2019 Feb 14.

Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, 71 Xinmin Street, Changchun, 130021, Jilin, China.

Hepatocellular carcinoma (HCC) is the most common liver cancer and has a poor prognosis. miR-302a is an important regulator of tumor occurrence and deterioration, while MAP3K2 and PBX3 genes are involved in cancer cell proliferation and apoptosis. In this study, the expression of miR-302a and MAP3K2/PBX3 were evaluated by qPCR in liver cancer cell lines. Read More

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http://dx.doi.org/10.1038/s41598-018-38435-0DOI Listing
February 2019

Indispensable role of β-arrestin2 in the protection of remifentanil preconditioning against hepatic ischemic reperfusion injury.

Sci Rep 2019 Feb 14;9(1):2087. Epub 2019 Feb 14.

Department of Anesthesiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Our previous study demonstrated that remifentanil, an opioid agonist, conferred profound liver protection during hepatic ischemia reperfusion injury (HIRI), in which Toll-like receptors (TLRs) played a crucial role in mediating the inflammatory responses. β-arrestin2, a well-known mu opioid receptor desensitizer, is also a negatively regulator of Toll-like receptor 4 (TLR4)-mediated inflammatory reactions in a mitogen-activated protein kinase (MAPK)-dependent manner. Using the rodent models of hepatic ischemia reperfusion injury both in wild type and TLR4 knockout (TLR4 KO) mice, we found that remifentanil preconditioning could inhibit the expression of TLR4 and reduce the inflammatory response induced by HIRI in wild type but not in TLR4 KO mice. Read More

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http://dx.doi.org/10.1038/s41598-018-38456-9DOI Listing
February 2019

Resveratrol Reduces Oxidative Stress and Apoptosis in Podocytes via Sir2-Related Enzymes, Sirtuins1 (SIRT1)/Peroxisome Proliferator-Activated Receptor γ Co-Activator 1α (PGC-1α) Axis.

Med Sci Monit 2019 Feb 15;25:1220-1231. Epub 2019 Feb 15.

Department of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, China (mainland).

BACKGROUND PGC-1α can be activated by deacetylation reactions catalyzed by SIRT1. Resveratrol is currently known as a potent activator of SIRT1. However, it is unknown whether the renal-protective effect of resveratrol is further related to activation of the podocyte SIRT1/PGC-1α pathway. Read More

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http://dx.doi.org/10.12659/MSM.911714DOI Listing
February 2019

Disruption of endolysosomal RAB5/7 efficiently eliminates colorectal cancer stem cells.

Cancer Res 2019 Feb 14. Epub 2019 Feb 14.

Gastroenterological Surgery, Graduate School of Medicine, Osaka University

Given that cancer stem cells (CSC) play a key role in drug resistance and relapse, targeting CSC remains a promising in cancer therapy. Here we show that RAB5/7, which are involved in the endolysosomal pathway, play key roles in the maintenance of CSC survival via regulation of the mitophagic pathway. Inhibition of RAB5/7 efficiently eliminated colorectal CSC and disrupted cancer foci. Read More

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http://dx.doi.org/10.1158/0008-5472.CAN-18-2192DOI Listing
February 2019