388,343 results match your criteria Apoptosis : an international journal on programmed cell death[Journal]


Advances in Delayed-Onset Muscle Soreness (DOMS): Part I: Pathogenesis and Diagnostics.

Sportverletz Sportschaden 2018 Dec 11;32(4):243-250. Epub 2018 Dec 11.

Muscle Research Center Erlangen, Interdisciplinary Center for Muscle Research, Friedrich-Alexander-University Erlangen-Nuremberg, Germany.

Delayed-onset muscle soreness (DOMS) is a type of ultrastructural muscle injury. The manifestation of DOMS is caused by eccentric or unfamiliar forms of exercise. Clinical signs include reduced force capacities, increased painful restriction of movement, stiffness, swelling, and dysfunction of adjacent joints. Read More

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December 2018

Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway.

Cell Physiol Biochem 2018 7;51(5):2359-2376. Epub 2018 Dec 7.

Background/aims: The aim of our study is to investigate the molecular mechanism by which mammalian STE20-like kinase 1 (Mst1) participates in renal I/R injury through modifying mitophagy and the AMPK-YAP signalling pathway.

Methods: WT mice and Mst1-knockout mice were subjected to renal ischaemia-reperfusion (I/R) in vivo. In vitro, the hypoxia-reoxygenation model was used with renal tubular epithelial cells to mimic renal I/R injury. Read More

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December 2018

Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation.

Cell Physiol Biochem 2018 7;51(5):2341-2358. Epub 2018 Dec 7.

Key Laboratory of the Model Animal Research, Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing,

Background/aims: This study investigated the effect of consecutive superovulation on the ovaries and established a premature ovarian failure (POF) model in mice.

Methods: The mouse POF model was induced by 5-15 consecutive superovulation treatments with pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) and prostaglandin F2α (PGF2α). Normal adult mice were compared with mice displaying natural ovarian aging. Read More

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December 2018

Circular RNA circPVT1 Promotes Proliferation and Invasion Through Sponging miR-125b and Activating E2F2 Signaling in Non-Small Cell Lung Cancer.

Cell Physiol Biochem 2018 7;51(5):2324-2340. Epub 2018 Dec 7.

Department of Prevention Medicine, School of Public Health, Mudanjiang Medical College, Mudanjiang City,

Background/aims: Circular RNAs (circRNAs) are key regulators in the development and progression of human cancers, however its role in non-small cell lung cancer (NSCLC) tumorigenesis is not well understood. The aim of this study is to identify the expression level of circPVT1 in NSCLC and further investigated its functional relevance with NSCLC progression both in vitro and in vivo.

Methods: Quantative real-time PCR was used for the measurement of circPVT1 in NSCLC specimens and cell lines. Read More

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December 2018

The Transient Receptor Potential Channel, Vanilloid 5, Induces Chondrocyte Apoptosis via Ca2+ CaMKII-Dependent MAPK and Akt/ mTOR Pathways in a Rat Osteoarthritis Model.

Cell Physiol Biochem 2018 7;51(5):2309-2323. Epub 2018 Dec 7.

Department of Orthopedic Surgery, ShengJing Hospital, China Medical University, ShenYang,

Background/aims: Chondrocyte apoptosis is a central pathological feature of cartilage in osteoarthritis (OA). Accumulating evidence suggests that calcium ions (Ca2+) are an important regulator of apoptosis. Previously, we reported that the transient receptor potential channel vanilloid (TRPV5) is upregulated in monoiodoacetic acid (MIA)-induced OA articular cartilage. Read More

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December 2018
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MicroRNA-128 Protects Dopamine Neurons from Apoptosis and Upregulates the Expression of Excitatory Amino Acid Transporter 4 in Parkinson's Disease by Binding to AXIN1.

Cell Physiol Biochem 2018 7;51(5):2275-2289. Epub 2018 Dec 7.

Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming,

Background/aims: Parkinson's disease (PD) is a frequently occurring condition that resulted from the loss of midbrain neurons, which synthesize the neurotransmitter dopamine. In this study, we established mouse models of PD to investigate the expression of microRNA-128 (miR-128) and mechanism through which it affects apoptosis of dopamine (DA) neurons and the expression of excitatory amino acid transporter 4 (EAAT4) via binding to axis inhibition protein 1 (AXIN1).

Methods: Gene expression microarray analysis was performed to screen differentially expressed miRNAs that are associated with PD. Read More

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December 2018
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circZMYM2 Competed Endogenously with miR-335-5p to Regulate JMJD2C in Pancreatic Cancer.

Cell Physiol Biochem 2018 7;51(5):2224-2236. Epub 2018 Dec 7.

Departments of Urology, the Third Affiliated Hospital of SooChow University, Changzhou,

Background/aims: We aimed to study the involvement of circZMYM2 (hsa_circ_0099999) in pancreatic cancer (PC) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circZMYM2 regulating miR-335-5p and JMJD2C.

Methods: CircRNA differential expressions in twenty PC samples and paired normal tissue samples were analyzed using Arraystar Human CircRNA microarray V1. CircZMYM2 expression level was determined via qRT-PCR. Read More

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December 2018

miR-494 Sensitizes Gastric Cancer Cells to TRAIL Treatment Through Downregulation of Survivin.

Cell Physiol Biochem 2018 7;51(5):2212-2223. Epub 2018 Dec 7.

Department of Internal Medicine, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou,

Background/aims: TNF-related apoptosis-inducing ligand (TRAIL) is a novel and low-toxic anti-tumor drug used for various cancers. However, cancer cells usually develop mechanisms to acquire the resistance against TRAIL. Among these changes, dysregulation of microRNAs (miRNAs) usually occurs in cancer cells and is responsible for induction of drug resistance. Read More

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December 2018

Deficiency of VCP-Interacting Membrane Selenoprotein (VIMP) Leads to G1 Cell Cycle Arrest and Cell Death in MIN6 Insulinoma Cells.

Cell Physiol Biochem 2018 7;51(5):2185-2197. Epub 2018 Dec 7.

Department of Medicine, The University of Chicago, Chicago, Illinois,

Background/aims: VCP-interacting membrane selenoprotein (VIMP), an ER resident selenoprotein, is highly expressed in β-cells, however, the role of VIMP in β-cells has not been characterized. In this study, we studied the relationship between VIMP deficiency and β-cell survival in MIN6 insulinoma cells.

Methods: To determine the role of VIMP in β-cells, lentiviral VIMP shRNAs were used to knock down (KD) expression of VIMP in MIN6 cells. Read More

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December 2018

Dexamethasone induces osteoblast apoptosis through ROS-PI3K/AKT/GSK3β signaling pathway.

Biomed Pharmacother 2018 Dec 8;110:602-608. Epub 2018 Dec 8.

Department of Orthopedics, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, Hubei, 430060, China. Electronic address:

Background: Osteoblasts play important roles in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone (Dex), a type of glucocorticoids (GCs), induces apoptosis of osteoblasts and leads to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of phosphatidylinositol 3-kinase/Protein kinase 3 (PI3K/AKT) and glycogen synthase kinase 3β (GSK3β) on Dex-induced osteoblasts apoptosis. Read More

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December 2018

Cumulus-oocyte interactions and programmed cell death in bovine embryos produced in vitro.

Theriogenology 2018 Nov 26;126:81-87. Epub 2018 Nov 26.

Laboratory of Molecular Biology, Faculty of Animal Science, University of Sao Paulo, São Paulo, Brazil.

This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage. Read More

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November 2018

Cardiovascular toxicity and anxiety-like behavior induced by deltamethrin in zebrafish (Danio rerio) larvae.

Chemosphere 2018 Dec 5;219:155-164. Epub 2018 Dec 5.

State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China. Electronic address:

Deltamethrin is widely used because of its low toxicity and high efficiency. Although its potential toxicity has been reported, its effects on cardiovascular system and motor behavior and its underlying mechanisms have remained unclear. In this study, the effects of deltamethrin on the development, cardiovascular system and motor behavior of zebrafish larvae and their possible mechanisms were evaluated using the transgenic zebrafish Tg (kdrl:mCherry) and Tg(myl7:GFP). Read More

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December 2018

Cadmium induces apoptotic program imbalance and cell cycle inhibitor expression in cultured human astrocytes.

Environ Toxicol Pharmacol 2018 Dec 3;65:53-59. Epub 2018 Dec 3.

Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand; Translational Medicine Graduate Program, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. Electronic address:

Cadmium is a highly neurotoxic heavy metal impairing neurogenesis and induces neurodegenerative disorders. Toxic concentrations of cadmium induce astrocytic apoptosis by depleting intracellular glutathione levels, elevating intracellular calcium levels, altering mitochondria membrane potentials, and activating JNK and PI3K/Akt signaling pathways. Cadmium suppresses cell proliferation in kidney epithelial cells, lung fibroblasts, and primary myelocytes; however, cadmium's effects on proteins regulating oxidative stress, apoptosis, and cell proliferation in astrocytes are less known. Read More

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December 2018

Lymphocyte-Specific Chromatin Accessibility Pre-determines Glucocorticoid Resistance in Acute Lymphoblastic Leukemia.

Cancer Cell 2018 Dec;34(6):906-921.e8

Children's Cancer Institute, Lowy Cancer Research Centre, UNSW, Sydney, NSW 2052, Australia. Electronic address:

Glucocorticoids play a critical role in the treatment of lymphoid malignancies. While glucocorticoid efficacy can be largely attributed to lymphocyte-specific apoptosis, its molecular basis remains elusive. Here, we studied genome-wide lymphocyte-specific open chromatin domains (LSOs), and integrated LSOs with glucocorticoid-induced RNA transcription and chromatin modulation using an in vivo patient-derived xenograft model of acute lymphoblastic leukemia (ALL). Read More

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December 2018

BH3-Mimetic Drugs: Blazing the Trail for New Cancer Medicines.

Cancer Cell 2018 Dec;34(6):879-891

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia. Electronic address:

Defects in apoptotic cell death can promote cancer and impair responses of malignant cells to anti-cancer therapy. Pro-survival BCL-2 proteins prevent apoptosis by keeping the cell death effectors, BAX and BAK, in check. The BH3-only proteins initiate apoptosis by neutralizing the pro-survival BCL-2 proteins. Read More

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December 2018

The microRNA miR-181c enhances chemosensitivity and reduces chemoresistance in breast cancer cells via down-regulating osteopontin.

Int J Biol Macromol 2018 Dec 8. Epub 2018 Dec 8.

Department of Internal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150081, China.

Acquired resistance to chemotherapy is a frequent challenge in cancer care and one of the leading causes for failing breast cancer therapies. There is accumulative clinical and experimental evidence indicating that microRNAs (miRNAs) play a crucial role in developing therapeutic resistance in cancer cells. We aimed to explore key miRNAs and associated mechanisms by which breast cancer develops chemoresistance. Read More

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December 2018
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Pep-1&Borneol-bifunctionalized carmustin-loaded micelles enhance anti-glioma efficacy through tumor targeting and BBB penetratings.

J Pharm Sci 2018 Dec 8. Epub 2018 Dec 8.

Department of Neurosurgery, School of Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, China. Electronic address:

Tumor-targeting and blood-brain barrier (BBB)-penetrating are highly desirable for the treatment of glioma. In this study, we developed Pep-1&borneol-bifunctionalized carmustin-loaded micelles (Pep-1/Bor/CMS-M) capable of targeting to IL-13 receptor-overexpressed glioma and penetrating the brain microvascular endothelial cells-associated physiologic barriers. Pep-1/Bor/CMS-M were nearly spherical particles with a diameter of 32. Read More

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December 2018
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A new human pyridinium metabolite of furosemide, inhibitor of mitochondrial complex I, is a candidate inducer of neurodegeneration.

Biochem Pharmacol 2018 Dec 8. Epub 2018 Dec 8.

Université Paris-Est, Laboratoire CRRET ERL-CNRS 9215, UPEC, F-94010, Créteil, France. Electronic address:

Pharmaceuticals and their by-products are increasingly a matter of concern, because of their unknown impacts on human health and ecosystems. The lack of information on these transformation products, which toxicity may exceed that of their parent molecules, makes their detection and toxicological evaluation impossible. Recently we characterized the Pyridinium of furosemide (PoF), a new transformation product of furosemide, the most widely used diuretic and an emerging pollutant. Read More

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December 2018

Protein S-glutathionylation: The linchpin for the transmission of regulatory information on redox buffering capacity in mitochondria.

Chem Biol Interact 2018 Dec 8. Epub 2018 Dec 8.

Protein S-glutathionylation reactions are a ubiquitous oxidative modification required to control protein function in response to changes in redox buffering capacity. These reactions are rapid and reversible and are, for the most part, enzymatically mediated by glutaredoxins (GRX) and glutathione S-transferases (GST). Protein S-glutathionylation has been found to control a range of cell functions in response to different physiological cues. Read More

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December 2018

Leptospira species promote a pro-inflammatory phenotype in human neutrophils.

Cell Microbiol 2018 Dec 10:e12990. Epub 2018 Dec 10.

Laboratory of Animal Viruses, Institute of Biotechnology and Molecular Biology, CONICET-UNLP, La Plata, Argentina.

Leptospirosis is a global zoonosis caused by pathogenic Leptospira. Neutrophils are key cells against bacterial pathogens but can also contribute to tissue damage. Since the information regarding the role of human neutrophils in leptospirosis is scant, we comparatively analyzed the human neutrophil's response to saprophytic Leptospira biflexa serovar Patoc (Patoc) and the pathogenic Leptospira interrogans serovar Copenhageni (LIC). Read More

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December 2018

Downregulation of miR-146a promotes cell migration in Helicobacter pylori-negative gastric cancer.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

microRNAs (miRs) are short noncoding RNAs that post-transcriptionally suppress gene expression. miR-146a acts as an oncogene or a tumor suppressor in various cancers, including gastric cancer, but it is unclear what determines whether miR-146a is oncogenic or tumor suppressive and the molecular mechanisms are still largely unknown. The aim of this study was to investigate the role of miR-146a in gastric cancer, by focusing on its expression in patients who were negative for Helicobacter pylori and its reduced and increased expression effect in vitro. Read More

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December 2018

Ginseng metabolite protopanaxadiol interferes with lipid metabolism and induces endoplasmic reticulum stress and p53 activation to promote cancer cell death.

Phytother Res 2018 Dec 9. Epub 2018 Dec 9.

The Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, USA.

Protopanaxadiol (PPD), a ginseng metabolite generated by the gut bacteria, was shown to induce colorectal cancer cell death and enhance the anticancer effect of chemotherapeutic agent 5-FU. However, the mechanism by which PPD promotes cancer cell death is not clear. In this manuscript, we showed that PPD activated p53 and endoplasmic reticulum (ER) stress and induced expression of BH3-only proteins Puma and Noxa to promote cell death. Read More

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December 2018

Salvianolic acid B inhibits the development of diabetic peripheral neuropathy by suppressing autophagy and apoptosis.

J Pharm Pharmacol 2018 Dec 7. Epub 2018 Dec 7.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objectives: The aim of this study was to evaluate the neuroprotective effects of SalB on high glucose (HG)-induced excessive autophagy and apoptosis in vitro.

Methods: The proliferation and apoptosis of RSC96 cells were determined using the MTT assay and flow cytometry, respectively. Western blot analysis was performed to examine the expression of autophagy and apoptosis-related proteins. Read More

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December 2018

Role of miR-486-5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF-β-activated kinase 1.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

The Department of Oncology, Hebei Chest Hospital, Shijiazhuang, Hebei, China.

Renal cell carcinoma (RCC) is a common kidney tumor in adults. The role of miR-486-5p in RCC is unknown. The aim of our study was to identify new targets regulated by miR-486-5p in RCC, to obtain a deeper insight into the network and to better understand the role of these microRNAs and their targets in carcinogenesis of RCC. Read More

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December 2018

Combined treatment with systemic resveratrol and resveratrol preconditioned mesenchymal stem cells, maximizes antifibrotic action in diabetic cardiomyopathy.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Physiology, Faculty of Medicine, Cairo University, Giza, Egypt.

Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-catenin signaling pathway in streptozotocin-induced DCM, and to evaluate effects of RSV preconditioning on MSCs therapeutic potential. The rats were divided into control (C, n = 8), diabetic (DM, n = 8), diabetic treated with systemic RSV (DM-RSV, n = 8), diabetic treated with RSV and nonconditioned MSCs (DM-RSV-MSCs, n = 8), diabetic treated with RSV and RSV-incubated with MSCs (DM-RSV-MSCc, n = 8) and diabetic treated with RSV-conditioned MSCs (DM-MSCc, n = 8). Echocardiography (Echo) showed significant improvement of cardiac functions in all groups treated with RSV either systemic or added in culture media. Read More

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December 2018

Activating Mas receptor protects human pulmonary microvascular endothelial cells against LPS-induced apoptosis via the NF-kB p65/P53 feedback pathways.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Critical Care Medicine, Shanghai Jiaotong University affiliated to The Sixth People's Hospital, Shanghai, China.

The balance between Ang II/AT1R and Ang-(1-7)/Mas plays a pivotal role in the development of lipopolysaccharides (LPS)-induced acute respiratory distress syndrome. However, the mechanisms underlying the balancing process still remain unclear. Here we investigated the roles of nuclear factor (NF)-κB and p53 in regulating AT1R and Mas expression. Read More

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December 2018

Simvastatin alleviates bone resorption in apical periodontitis possibly by inhibition of mitophagy-related osteoblast apoptosis.

Int Endod J 2018 Dec 11. Epub 2018 Dec 11.

Department of Dentistry, School of Dentistry, National Taiwan University, Taipei.

Aim: To assess the connection between mitophagy and hypoxia-induced apoptosis in osteoblasts and whether simvastatin alleviates bone resorption in apical periodontitis through modulation of mitophagy-related apoptosis.

Methodology: Hypoxia-induced generation of reactive oxygen species in mitochondria and changes in mitochondrial membrane potential were evaluated respectively by MitoSOX and JC-1 fluorescence dye signaling. Accumulation of mitophagy markers PTEN-induced putative kinase 1 (PINK1) and Parkin in mitochondria was examined by Western blotting and immunofluorescence microscopy. Read More

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December 2018

Downregulation of microRNA-23b protects against ischemia-reperfusion injury via p53 signaling pathway by upregulating MDM4 in rats.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Anhui Key Laboratory of Tissue Transplantation, Bengbu, China.

Total knee arthroplasty is a commonly performed safe procedure and typically executed in severe knee arthritis, but it also triggers ischemia-reperfusion injury (IRI). More recently, microRNAs (miRs) have been reported to play a contributory role in IRI through the key signaling pathway. Hence, the current study aimed to investigate the effect and specific mechanism of microRNA-23b (miR-23b), murine double minute 4 (MDM4), and the p53 signaling pathway in IRI rat models. Read More

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December 2018

miR-133a-3p suppresses cell proliferation, migration, and invasion and promotes apoptosis in esophageal squamous cell carcinoma.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Oncology, Linyi People's Hospital, Linyi, Shandong, China.

Objective: This study aimed to investigate the expression levels of miR-133a-3p and collagen type I α 1 (COL1A1) in esophageal squamous cell carcinoma (ESCC) to find out the relationship between miR-133a-3p and COL1A1 and their influence on ESCC propagation, migration, invasion, and apoptosis.

Methods: The messenger RNA expression levels of miR-133a-3p and COL1A1 in ESCC were detected by quantitative reverse-transcription polymerase chain reaction. The expression of COL1A1 protein was examined via western blot analysis and immunohistochemistry assay. Read More

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December 2018

Astrocytic insulin-like growth factor-1 prevents excitotoxic downregulation of adenosine deaminase acting on RNA in calcium dynamics.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Neurosurgery, Shanghai Pudong New Area People's Hospital Shanghai, China.

Background: A-to-I RNA editing catalyzed by the adenosine deaminase acting on RNA (ADAR) 1 represents an RNA-based recoding mechanism implicated in a variety of cellular processes like intracellular homeostasis. Previous studies have demonstrated that the expression of ADAR1 is increased in glioma, and ADAR1 short hairpin RNA affects regulated cellular exocytosis. This indicates that ADAR1 might be involved in cellular proliferation and/or apoptosis. Read More

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December 2018

PML/RARA inhibits expression of HSP90 and its target AKT.

Br J Haematol 2018 Dec 9. Epub 2018 Dec 9.

Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy.

Essential for cell survival, the 90 kD Heat Shock Proteins (HSP90) are molecular chaperons required for conformational stabilization and trafficking of numerous client proteins. Functional HSP90 is required for the stability of AKT, a serine-threonine kinase phosphorylated in response to growth factor stimulation. AKT plays a crucial regulatory role in differentiation, cell cycle, transcription, translation, metabolism and apoptosis. Read More

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December 2018

Activation of TGF-β1/Smad3 signaling pathway inhibits the development of ovarian follicle in polycystic ovary syndrome by promoting apoptosis of granulosa cells.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China.

Objective: To investigate the role of the transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway in development of ovarian follicle by promoting apoptosis of granulosa cells in polycystic ovary syndrome (PCOS).

Methods: A total of 54 female rats were obtained and randomly assigned into the PCOS (n = 27) and control groups ( n = 27). PCOS rat models were constructed using the dehydroepi-androsterone method to observe ovarian morphology and ultrastructure. Read More

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December 2018

Downregulation of long noncoding RNA H19 rescues hippocampal neurons from apoptosis and oxidative stress by inhibiting IGF2 methylation in mice with streptozotocin-induced diabetes mellitus.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, China.

The diabetes mellitus (DM)-induced reduction of neurogenesis in the hippocampus is consequently accompanied by cognitive decline. The present study set out to define the critical role played by long noncoding RNA H19 (lncRNA H19) in the apoptosis of hippocampal neurons, as well as oxidative stress (OS) in streptozotocin (STZ)-induced DM mice through regulation of insulin-like growth factor 2 (IGF2) methylation. The expression of lncRNA H19 in the hippocampal neurons and surviving neurons were detected. Read More

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December 2018

Diacylglycerol kinase α-selective inhibitors induce apoptosis and reduce viability of melanoma and several other cancer cell lines.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan.

Diacylglycerol (DG) kinase (DGK), which phosphorylates DG to generate phosphatidic acid (PA), consists of ten isozymes (α-к). Recently, we identified a novel small molecule inhibitor, CU-3, that selectively inhibits the activity of the α isozyme. In addition, we newly obtained Compound A, which selectively and strongly inhibits type I DGKs (α, β, and γ). Read More

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December 2018

The methanolic extract of Guibourtia tessmannii (caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement of TRPV1 channels.

Andrologia 2018 Dec 10:e13216. Epub 2018 Dec 10.

Department of Physiology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia.

This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca ] ), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL-97 (R2C) tumour Leydig cells. The cells were divided into nine groups and treated as follows: (a)-Control, (b)-Capsazepine (CPZ, 0.1 mM, a TRPV1 channel blocker), (c)-Capsaicin (CAP, 0. Read More

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December 2018

Long noncoding RNA ATB participates in the development of renal cell carcinoma by downregulating p53 via binding to DNMT1.

J Cell Physiol 2018 Dec 8. Epub 2018 Dec 8.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.

Long noncoding RNA (lncRNA) exerts an essential role in the pathological processes of many diseases. Our previous study found that lncRNA ATB was highly expressed in renal cell carcinoma (RCC). Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and migration-related assays were conducted to access the regulatory effects of lncRNA ATB on proliferative and migratory capacities of RCC cells. Read More

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December 2018

Protective effects of plant-derived natural products on renal complications.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Diabetic nephropathy is the leading cause of renal failure worldwide. This debilitating disorder has several underlying pathophysiologic mechanisms, and therefore a variety of pharmacologic agents have been developed to prevent or treat diabetic nephropathy; however, synthetic drugs may possess unfavorable side effects. In response to this, the global use of herbal-based pharmacologic agents is increasing among diabetic patients. Read More

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December 2018

Astragaloside IV inhibits cell proliferation in vulvar squamous cell carcinoma through the TGF-β/Smad signaling pathway.

Dermatol Ther 2018 Dec 7:e12802. Epub 2018 Dec 7.

From the Department of Gynecology, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning, P.R. China.

Objective: To explore the inhibition of the proliferation of vulvar squamous cell carcinoma (VSCC) by astragaloside IV.

Methods: MTT examined the cell proliferation of VSCC. Flow cytometry analyzed cell cycle and apoptosis. Read More

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December 2018

Cyclin-dependent kinase 9 promotes cervical cancer development via AKT2/p53 pathway.

IUBMB Life 2018 Dec 11. Epub 2018 Dec 11.

Department of Gynecologic Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Aberrant activation of cyclin-dependent kinase 9 (CDK9) is widespread in human cancers. However, the underlying mechanisms of CDK9 activation and the therapeutic potential of CDK9 inhibition in cervical cancer remain largely unknown. Here, we report that CDK9 is gradually upregulated during cervical lesion progression and regulated by HPV16 E6. Read More

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December 2018

Impact of Oxidative Metabolism on the Cytotoxic and Genotoxic Potential of Genistein in Human Colon Cancer Cells.

Mol Nutr Food Res 2018 Nov 22:e1800635. Epub 2018 Nov 22.

Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria.

Scope: Genistein (GEN) is known to be genotoxic via targeting topoisomerase-II (TOPII). Oxidative metabolism of GEN is shown to generate hydroxylated metabolites with catecholic structures. The present study focuses on the impact of oxidative metabolism of GEN, exemplified for 3'-hydroxygenistein (3'-OH-GEN) and 6-hydroxygenistein (6-OH-GEN), on topoisomerase interference and the resulting genotoxic potential in HT-29 human colon carcinoma cells. Read More

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November 2018

NPAS2 regulates proliferation of acute myeloid leukemia cells via CDC25A-mediated cell cycle progression and apoptosis.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Promoted proliferation and associated suppression of apoptosis at various stages of myeloid differentiation are well-known features of acute myeloid leukemia (AML), but understanding of the molecular processes involved remains limited. As a crucial circadian agent, neuronal PAS domain protein 2 (NPAS2) is widely recognized as a promising predictor of clinical outcome in various malignancies. Nevertheless, the understanding of its influence on AML is insufficient. Read More

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December 2018

LncRNA MALAT1 protects cardiomyocytes from isoproterenol-induced apoptosis through sponging miR-558 to enhance ULK1-mediated protective autophagy.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Henan Province People's Hospital, People's Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital Zhengzhou, Henan, China.

Investigating the molecular mechanisms of myocardial infarction (MI) and subsequent heart failure have gained considerable attention worldwide. Long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been previously demonstrated to regulate the proliferation and metastasis of several tumors. However, little is known about the effects of MALAT1 in MI and in regulating the cell date after MI. Read More

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December 2018

Influence of colonic dialysis using Gubenxiezhuo on the distribution of gut microflora in uremia rats.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Nephrology, Minhang Hospital,Fudan University, Shanghai, China.

Objective: This study aimed to explore the underlying function of Gubenxiezhuo dialysis on the distribution of gut microflora uremia.

Methods: A uremia rat model was constructed, and the morphology of renal tissue was determined using the hematoxylin-eosin (H&E) staining. Moreover, the blood samples were collected and the expression of IL-1β, IL-6, and CRP was determined using enzyme-linked immunosorbent assay. Read More

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December 2018

Engagement of Fas differentially regulates the production of LPS-induced pro-inflammatory cytokines and type I Interferons.

FEBS J 2018 Dec 11. Epub 2018 Dec 11.

Departments of Pathology, University College Cork, Cork, Ireland.

Fas (CD95) signalling is best known for its role in apoptosis, however, recent reports have shown it to be involved in other cellular responses as well, including inflammation. Fas and its adaptor protein FADD are known to negatively regulate LPS-induced pro-inflammatory responses, but their role in LPS-induced type I Interferon production is unknown. Here, we demonstrate that Fas engagement on macrophages, using an agonistic Fas antibody CH11, augments LPS-induced NF-κB responses, causing increased production of TNFα, IL-8, IL-6 and IL-12. Read More

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December 2018

Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs.

Cell Biol Int 2018 Dec 11. Epub 2018 Dec 11.

Department of Cardiology, Yantai Affiliated Hospital of Binzhou Medical University, yantai, Shandong 264100, P. R. China.

Long noncoding RNAs (lncRNAs) have been found to play important roles in nearly all biological processes. However, the functions of the majority of LncRNAs are not fully clear. Here we evaluated the function of lncRNA OGFRP1, which has not been previously annotated, in human coronary artery endothelial cells (HCAECs). Read More

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December 2018

Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Geriatric Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Oxidized low-density lipoprotein (Ox-LDL)-induced endothelial cell injury plays a crucial role in the pathogenesis of atherosclerosis (AS). Plasma galectin-3 (Gal-3) is elevated inside and drives diverse systemic inflammatory disorders, including cardiovascular diseases. However, the exact role of Gal-3 in ox-LDL-mediated endothelial injury remains unclear. Read More

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December 2018

Plumbagin induces autophagy and apoptosis of SMMC-7721 cells in vitro and in vivo.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Physiology, Guangxi University of Chinese Medicine, Nanning, Guangxi, China.

Plumbagin (PL), an active naphthoquinone compound, has been demonstrated to be a potential anticancer agent. However, the underlying anticancer mechanism is not fully understood. In this study, the human hepatocellular carcinoma (HCC) SMMC-7721 cell line was studied in an in vitro model. Read More

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December 2018

Gossypin inhibits gastric cancer growth by direct targeting of AURKA and RSK2.

Phytother Res 2018 Dec 10. Epub 2018 Dec 10.

China-US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China.

Gossypin is a flavone extracted from Hibiscus vitifolius, which has been reported to exhibit anti-inflammatory, antioxidant, and anticancer activities. However, the anticancer properties of gossypin and its molecular mechanism of action against gastric cancer have not been fully investigated. In the present study, we report that gossypin is an Aurora kinase A (AURKA) and RSK2 inhibitor that suppresses gastric cancer growth. Read More

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December 2018
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In vitro modulatory effect of dehydroepiandrosterone sulfate on apoptosis and expression of apoptosis-related genes in patients with systemic lupus erythematosus.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Rheumatology Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Objectives: Systemic lupus erythematosus‌ (SLE) is an autoimmune disease characterized by hyperactive B cells that produce various autoantibodies. Sex hormones have been documented to influence the development of SLE, in which women with SLE have low plasma level of dehydroepiandrosterone sulfate (DHEAS). A strong conclusion about the effect of DHEAS on apoptosis in SLE patients has not been provided. Read More

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December 2018

Loss of Lgr4 inhibits differentiation, migration and apoptosis, and promotes proliferation in bone mesenchymal stem cells.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Shanghai Fengxian District Central Hospital and East China Normal University Joint Center for Translational Medicine, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.

The key signaling networks regulating bone marrow mesenchymal stem cells (BMSCs) are poorly defined. Lgr4, which belongs to the leucine-rich repeat-containing G protein-coupled receptor (LGR) family, is widely expressed in multiple tissues from early embryogenesis to adulthood. We investigated whether Lgr4 functions in BMSCs and in osteogenesis, adipogenesis, and skeletal myoblasts, using mice with a β-geo gene trap inserted into the Lgr4 gene. Read More

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December 2018