399,348 results match your criteria Apoptosis : an international journal on programmed cell death[Journal]


Fisetin and 5-Fluorouracil: Effective Combination for PIK3CA-Mutant Colorectal Cancer.

Int J Cancer 2019 Apr 24. Epub 2019 Apr 24.

Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA.

The normal colon epithelium is transformed into its neoplastic counterpart through a series of genetic alterations in driver genes including activating mutations in PIK3CA. Treatment often involves surgery followed by 5-fluorouracil (5-FU) based therapy, which has limited efficiency and serious side effects. We sought to determine whether fisetin, a dietary flavonoid, alone or in combination with 5-FU affected tumorigenesis in the mammalian intestine. Read More

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http://dx.doi.org/10.1002/ijc.32367DOI Listing

Side Effects of Frequently Used Antihypertensive Drugs on Wound Healing in vitro.

Skin Pharmacol Physiol 2019 Apr 24;32(3):162-172. Epub 2019 Apr 24.

Institute for Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Witten, Germany.

Background: The number of patients who has a daily intake of antihypertensive drugs is rising, due to an also rising prevalence of lifestyle diseases. Interestingly, knowledge about effects of these drugs in terms of wound healing is low.

Objective: Based on a few differing studies, the idea arose that antihypertensives may have side effects on wound healing. Read More

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http://dx.doi.org/10.1159/000499433DOI Listing
April 2019
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PLAC8 is overexpressed and regulates cell proliferation in low-grade human PanNET.

Neuroendocrinology 2019 Apr 25. Epub 2019 Apr 25.


Background/Aims: Many aspects of the biology of pancreatic neuroendocrine tumors (PanNETs), including determinants of proliferative, invasive and metastatic potential, remain poorly understood. Placenta-specific 8 (PLAC8), a gene with unknown molecular function, has been reported to have tumor-promoting roles in different human malignancies, including exocrine pancreatic cancer. Since preliminary data suggested deregulation of PLAC8 expression in PanNET, we have performed detailed analyses of PLAC8 expression and function in human PanNET. Read More

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http://dx.doi.org/10.1159/000500541DOI Listing

FA-NBs-IR780: Novel multifunctional nanobubbles as molecule-targeted ultrasound contrast agents for accurate diagnosis and photothermal therapy of cancer.

Cancer Lett 2019 Apr 21. Epub 2019 Apr 21.

Department of Ultrasound Medicine, Tang Du Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, China. Electronic address:

Early accurate diagnosis and targeted therapy for cancer are essential to improve the prognosis of patients. With the emergence of molecular imaging, molecule-targeted ultrasound imaging for the non-invasive and precise detection of cancer has attracted increased attention. The investigation of molecule-targeted ultrasound contrast agents (UCAs) with excellent performance is urgently needed. Read More

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http://dx.doi.org/10.1016/j.canlet.2019.04.023DOI Listing

Chemical composition and cytotoxic screening of Musa cavendish green peels extract: Antiproliferative activity by activation of different cellular death types.

Toxicol In Vitro 2019 Apr 21. Epub 2019 Apr 21.

Emergency Medicine Department, Medical School, University of São Paulo, Av. Dr. Arnaldo, 455 - Cerqueira César, São Paulo, São Paulo CEP 01246-903, Brazil.

Musa cavendish, commonly known as banana, is a fruit with nutritional and therapeutic properties. We investigated the chemical composition and in vitro cytotoxic effect of M. cavendish green peel extract (MHE) on cancer cells for the first time. Read More

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http://dx.doi.org/10.1016/j.tiv.2019.04.020DOI Listing

Glutamine deprivation induces metabolic adaptations associated with beta cell dysfunction and exacerbate lipotoxicity.

Mol Cell Endocrinol 2019 Apr 21. Epub 2019 Apr 21.

School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Perth, Western Australia, 6845, Australia. Electronic address:

Studies have reported that plasma glutamine is reduced in type 2 diabetes (T2D) patients. Glutamine supplementation improves glycaemic control, however the mechanisms are unclear. Here, we evaluated in vitro the pancreatic beta cell bioenergetic and insulin secretory responses to various levels of glutamine availability, or treatment in the presence of an inhibitor of intracellular glutamine metabolism. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.013DOI Listing

Development of a Cx46 Targeting Strategy for Cancer Stem Cells.

Cell Rep 2019 Apr;27(4):1062-1072.e5

Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH 44195, USA; Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address:

Gap-junction-mediated cell-cell communication enables tumor cells to synchronize complex processes. We previously found that glioblastoma cancer stem cells (CSCs) express higher levels of the gap junction protein Cx46 compared to non-stem tumor cells (non-CSCs) and that this was necessary and sufficient for CSC maintenance. To understand the mechanism underlying this requirement, we use point mutants to disrupt specific functions of Cx46 and find that Cx46-mediated gap-junction coupling is critical for CSCs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22111247193041
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http://dx.doi.org/10.1016/j.celrep.2019.03.079DOI Listing
April 2019
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Downregulation of microRNA-200c-3p reduces damage of hippocampal neurons in epileptic rats by upregulating expression of RECK and inactivating the AKT signaling pathway.

Chem Biol Interact 2019 Apr 21. Epub 2019 Apr 21.

Department of Pediatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, PR China.

Objective: The aim of this study is to investigate the role of mircoRNA-200c-3p (miR-200c-3p) on hippocampal neuron injury in epileptic rats through the regulation of the AKT signaling pathway by targeting RECK.

Methods: The epilepsy rat model was induced by intraperitoneal injection of lithium chloride-pilocarpine. Successful modeled rats were injected with miR-200c-3p inhibitors, inhibitors NC, siRNA-negative control (NC) and RECK-siRNA. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.04.027DOI Listing

Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264.7 cells.

IUBMB Life 2019 Apr 24. Epub 2019 Apr 24.

Department of Pulmonary, Anhui Geriatric Institute, the First Affiliated Hospital of Anhui Medical University, Hefei, China.

Melatonin is one of the main hormones that regulate biological rhythms and have immunomodulation, anti-inflammatory, and antioxidation functions. In this study, we aimed to explore the effect of melatonin on the autophagy, apoptosis, and inflammatory reaction of macrophages (RAW264.7 cells) stimulated by nanosilica. Read More

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http://dx.doi.org/10.1002/iub.2055DOI Listing

miR-146a-5p Enhances Hepatitis B Virus Replication Through Autophagy to Promote Aggravation of Chronic Hepatitis B.

IUBMB Life 2019 Apr 24. Epub 2019 Apr 24.

Department of Infectious Diseases, Key Laboratory of Hunan Viral Hepatitis, Xiangya Hospital, Central South University, Changsha, Hunan, China.

The objective of this study was to investigate the mechanism by which miR-146a-5p mediated autophagy and hepatitis B virus (HBV) replication. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the mRNA expression levels of miR-146a-5p and X-linked inhibitor of apoptosis (XIAP) and HBV DNA and RNA. The protein expression levels of XIAP, IκB-α, murine double minute 2 oncoprotein (MDM2) and p53, the phosphorylation of p65, and the conversion of light chain 3 (LC3)-I to LC3-II were detected by Western blotting. Read More

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http://dx.doi.org/10.1002/iub.2044DOI Listing

Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells.

PLoS One 2019 24;14(4):e0215905. Epub 2019 Apr 24.

Institute for Integrative Physiology, Center for Systems Biology of O2 Sensing, Biological Sciences Division, The University of Chicago, Chicago, Illinois, United States of America.

The alpha subunit of the voltage gated human ether-a-go-go-related (hERG) potassium channel regulates cell excitability in a broad range of cell lines. HERG channels are also expressed in a variety of cancer cells and control cell proliferation and apoptosis. Hypoxia, a common feature of tumors, alters gating properties of hERG currents in SH-SY5Y neuroblastoma cells. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215905PLOS
April 2019
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(De)glutamylation and cell death in Leishmania parasites.

PLoS Negl Trop Dis 2019 Apr 24;13(4):e0007264. Epub 2019 Apr 24.

Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.

Trypanosomatids are flagellated protozoan parasites that are very unusual in terms of cytoskeleton organization but also in terms of cell death. Most of the Trypanosomatid cytoskeleton consists of microtubules, forming different substructures including a subpellicular corset. Oddly, the actin network appears structurally and functionally different from other eukaryotic actins. Read More

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http://dx.doi.org/10.1371/journal.pntd.0007264DOI Listing

Autophagy, Apoptosis, and Mitochondria: Molecular Integration and Physiological Relevance in Skeletal Muscle.

Am J Physiol Cell Physiol 2019 Apr 24. Epub 2019 Apr 24.

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.

Apoptosis and autophagy are processes resulting from the integration of cellular stress and death signals. Their individual importance is highlighted by the lethality of various mouse models missing apoptosis or autophagy related genes. In addition to their independent roles, significant overlap exists with respect to the signals which stimulate these processes as well as their effector consequences. Read More

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http://dx.doi.org/10.1152/ajpcell.00261.2018DOI Listing

Upregulated MicroRNA-141 Suppresses Epithelial-Mesenchymal Transition and Lymph Node Metastasis via HOXC6-Dependent TGF-β Signaling Pathway in Laryngeal Cancer.

Am J Physiol Cell Physiol 2019 Apr 24. Epub 2019 Apr 24.

Department of Medicine Oncology, Linyi People's Hospital, Linyi 276003, P.R. China.

Laryngeal cancer ranks as the foremost head and neck malignant tumor. The abnormal expression of microRNAs (miRNAs) has been reported due to its role in cancer development with ability to regulate proliferation and apoptosis of cancer cells. This study investigated the roles of microRNA-141 (miR-141), Homeobox C6 (HOXC6), and the transforming growth factor-β (TGF-β) signaling pathway in epithelial-mesenchymal transition (EMT) and lymph node metastasis in laryngeal cancer. Read More

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http://dx.doi.org/10.1152/ajpcell.00291.2018DOI Listing

Investigation of the effect of P14 promoter aberrant methylation on the biological function of human lung cancer cells.

Thorac Cancer 2019 Apr 24. Epub 2019 Apr 24.

Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Background: This study was conducted to investigate the effect of P14 promoter aberrant methylation on the biological function of human lung adenocarcinoma cells.

Methods: We used nested methylation-specific PCR (NMSP) to detect the methylation status of the p14ARF promoter region in SPCA1 and BEAS2B cell lines. The experimental groups were treated with 5-aza-2'-deoxycytidine (5-Aza). Read More

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http://dx.doi.org/10.1111/1759-7714.13082DOI Listing

Localized RNA interference therapy to eliminate residual lung cancer after incomplete microwave ablation.

Thorac Cancer 2019 Apr 24. Epub 2019 Apr 24.

Department of Minimally Invasive Interventional Center, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: This study evaluated the safety and efficacy of localized injection of polyethylene glycol (PEG)-hyperbranched polyethyleneimine (PEI)-EGFR-small interfering RNA (siRNA) nanocomposites as a treatment for residual lung cancer after incomplete microwave ablation (MWA).

Methods: Human lung cancer cell lines with high and low EGFR expression were selected for the study. The effects of PEG-PEI-EGFR-siRNA nanocomposite transfection on the proliferation, migration, and apoptosis of lung cancer cells were verified. Read More

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http://dx.doi.org/10.1111/1759-7714.13079DOI Listing

miR-144 reverses cisplatin resistance in cervical cancer via targeting LHX2.

J Cell Biochem 2019 Apr 24. Epub 2019 Apr 24.

Department of Radiation Oncology, First Hospital of Xi'an Jiaotong Univesity, Xi'an, Shaanxi, China.

Mounting evidence showed that microRNAs involve in development and chemoresistance of various human cancers. We explored the roles and mechanisms of miR-144 in resistance to cisplatin (CDDP) of cervical cancer cells. miR-144 and LIM homeobox 2 (LHX2) expression in CDDP-resistant and the parental cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis, respectively. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28763
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http://dx.doi.org/10.1002/jcb.28763DOI Listing
April 2019
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Protective impacts of long noncoding RNA taurine-upregulated 1 against lipopolysaccharide-evoked injury in MRC-5 cells through inhibition of microRNA-127.

J Cell Biochem 2019 Apr 24. Epub 2019 Apr 24.

Department of Pediatrics, Jining No.1 People's Hospital, Jining, China.

Background: Pneumonia is a respiratory disease, which is triggered by pathogenic microorganisms or physical/chemical factors. Increasing evidence confirmed the vital impacts of long noncoding RNAs on various inflammatory diseases. Nonetheless, the influence of taurine-upregulated 1 (TUG1) in pneumonia remains vague. Read More

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http://dx.doi.org/10.1002/jcb.28755DOI Listing

microRNA-messenger RNA regulatory network of esophageal squamous cell carcinoma and the identification of miR-1 as a biomarker of patient survival.

J Cell Biochem 2019 Apr 24. Epub 2019 Apr 24.

Department of Pathology Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Emerging evidence indicates that microRNAs (miRNAs) play an important role in tumor carcinogenesis and progression by targeting gene expression. The goal of this study was to comprehensively analyze the vital functional miRNAs and their target genes in esophageal squamous cell carcinoma (ESCC) and to explore the clinical significance and mechanisms of miR-1 in ESCC. First, the miRNA and messenger RNA (mRNA) expression profiles of ESCC were determined with microarray technology. Read More

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http://dx.doi.org/10.1002/jcb.28166DOI Listing

Downregulation of miRNA-30a enhanced autophagy in osthole-alleviated myocardium ischemia/reperfusion injury.

J Cell Physiol 2019 Apr 24. Epub 2019 Apr 24.

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Osthole could alleviate myocardial ischemia/reperfusion (I/R) injury. However, the underlying mechanism remains unclear. In this study, we explored whether microRNA (miR)-30a and its target autophagy marker Beclin-1 involved in the osthole protective role in the rat and cells myocardial I/R injury models. Read More

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http://dx.doi.org/10.1002/jcp.28556DOI Listing

Neuroprotective and long term potentiation improving effects of vitamin E in juvenile hypothyroid rats.

Int J Vitam Nutr Res 2019 Apr 24:1-13. Epub 2019 Apr 24.

8 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Protective effects of vitamin E (Vit E) on long term potentiation (LTP) impairment, neuronal apoptosis and increase of nitric oxide (NO) metabolites in the hippocampus of juvenile rats were examined. The rats were grouped (n=13) as: (1) control; (2) hypothyroid (Hypo) and (3) Hypo-Vit E. Propylthiouracil (PTU) was given in drinking water (0. Read More

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http://dx.doi.org/10.1024/0300-9831/a000533DOI Listing

Camel Whey Protein Disrupts the Cross-Talk Between PI3K and BCL-2 Signals and Mediates Apoptosis in Primary Acute Myeloid Leukemia Cells.

Nutr Cancer 2019 Apr 24:1-15. Epub 2019 Apr 24.

f Deanship of Scientific Research , King Saud University , Riyadh , Saudi Arabia.

In the present study, we investigated the impact of camel whey protein (CWP) on the survival of primary acute myeloid leukemia (AML) cells that were isolated from 20 patients diagnosed with AML. We found that CWP induced apoptosis in the primary AML cells without affecting the normal PBMCs that were isolated from healthy individuals, as determined by PI/annexin V double staining followed by flow-cytometry analysis. Furthermore, we demonstrated that these primary AML cells exhibited aberrant phosphorylation of AKT, mTOR and STAT3. Read More

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http://dx.doi.org/10.1080/01635581.2019.1595054DOI Listing

Anti-miRNA21 and resveratrol-loaded polysaccharide-based mesoporous silica nanoparticle for synergistic activity in gastric carcinoma.

J Drug Target 2019 Apr 24:1-31. Epub 2019 Apr 24.

b Department of Endoscopy Center , China-Japan Union Hospital of Jilin University , Changchun , Jilin , China , 130033.

Anti-miR21 and resveratrol (RSV)-loaded mesoporous silica nanoparticles conjugated with hyaluronic acid (HA) were developed to enhance therapeutic efficacy in gastric carcinoma. The surface conjugation of HA, which acts as a targeting ligand to the overexpressed CD44 receptor on gastric cancer cells, was clearly identified by the presence of a greyish shell on the dark mesoporous silica nanoparticles. Confocal laser-scanning microscopy and flow cytometry analysis showed higher cellular internalization of HA/RSVmirNP compared to RSVmirNP. Read More

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http://dx.doi.org/10.1080/1061186X.2019.1610766DOI Listing

Evolving evidences of calreticulin as a pharmacological target in neurological disorders.

ACS Chem Neurosci 2019 Apr 24. Epub 2019 Apr 24.

Calreticulin (CALR), a lectin-like ER chaperone, was initially known only for its house-keeping function, but today it is recognized for many versatile roles in different compartments of a cell. Apart from canonical roles in protein folding and calcium homeostasis it performs a variety of non-canonical roles, majorly in CNS development. In past, studies have linked Calreticulin with various other biological components which are detrimental in deciding the fate of neurons. Read More

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http://pubs.acs.org/doi/10.1021/acschemneuro.9b00158
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http://dx.doi.org/10.1021/acschemneuro.9b00158DOI Listing
April 2019
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Radiation-induced sensitivity of tissue-resident mesenchymal stem cells in the head and neck region.

Head Neck 2019 Apr 24. Epub 2019 Apr 24.

Department of Otorhinolaryngology, Klinikum der Universitaet Muenchen, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany.

Background: Tissue-resident mesenchymal stem cells (MSCs) possess the ability to migrate to areas of inflammation and promote the regeneration of damaged tissue. However, it remains unclear how radiation influences this capacity of MSC in the head and neck region.

Methods: Two types of MSCs of the head and neck region (mucosa [mMSC] and parotid gland [pMSC]) were isolated, cultured and exposed to single radiation dosages of 2 Gy/day up to 10 days. Read More

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http://dx.doi.org/10.1002/hed.25768DOI Listing

DHA protects PC12 cells against oxidative stress and apoptotic signals through the activation of the NFE2L2/HO-1 axis.

Int J Mol Med 2019 Jun 23;43(6):2523-2531. Epub 2019 Apr 23.

A. Gemelli University Polyclinic Foundation, Italy IRCCS, I‑00168 Rome, Italy.

Docosahexaenoic acid (DHA) is an omega‑3 polyunsaturated fatty acid, derived mainly from fish oil. It is well known that DHA is present in high concentrations in nervous tissue and plays an important role in brain development and neuroprotection. However, the molecular mechanisms underlying its role remain to be fully elucidated. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4170DOI Listing

Global brain ischemia in rats is associated with mitochondrial release and downregulation of Mfn2 in the cerebral cortex, but not the hippocampus.

Int J Mol Med 2019 Jun 16;43(6):2420-2428. Epub 2019 Apr 16.

Biomedical Center and Institute of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, SK‑03601 Martin, Slovak Republic.

Mitochondria are crucial for neuronal cell survival and death through their functions in ATP production and the intrinsic pathway of apoptosis. Mitochondrial dysfunction is considered to play a central role in several serious human diseases, including neurodegenerative diseases, such as Parkinson's and Alzheimer's disease and ischemic neurodegeneration. The aim of the present study was to investigate the impact of transient global brain ischemia on the expression of selected proteins involved in mitochondrial dynamics and mitochondria‑associated membranes. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4168DOI Listing

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

Int J Mol Med 2019 Jun 12;43(6):2341-2351. Epub 2019 Apr 12.

Department of Cosmetic Dentistry, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.

Toll‑like receptor 4 (TLR4) is a transmembrane receptor responsible for the activation of a number of signal transduction pathways. Despite its involvement in inflammatory processes, the regulation of TLR4 signaling in human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions remains to be fully elucidated. The present study aimed to clarify the regulatory mechanisms of the TLR4 signaling pathway and its role in the differentiation of hPDLSCs under inflammatory conditions. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4165DOI Listing

Lycopene restores the effect of ischemic postconditioning on myocardial ischemia‑reperfusion injury in hypercholesterolemic rats.

Int J Mol Med 2019 Jun 15;43(6):2451-2461. Epub 2019 Apr 15.

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.

Ischemic postconditioning (IPoC) has been demonstrated to prevent myocardial ischemia‑reperfusion injury (MIRI), but its cardioprotective effect is abrogated by hypercholesterolemia. The aim of the present study was to determine whether lycopene (LP), a type of carotenoid, can restore the cardioprotective effect of IPoC in hypercholesterolemic rats. Male Wistar rats were fed a cholesterol‑enriched diet for 12 weeks to establish a hypercholesterolemic model. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4166DOI Listing

Regulation of Fn14 stability by SCF during septic acute kidney injury.

Am J Physiol Renal Physiol 2019 Apr 24. Epub 2019 Apr 24.

Intensive Care Unit, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, China.

Acute kidney injury (AKI) initiated by sepsis remains a thorny problem despite recent advancements in its clinical management. Having been found to be activated during AKI, Fn14 may be a potential therapeutic target due to its involvement in the molecular basis of injury. Here we report that LPS induces apoptosis of tubular MCT cells mediated by Fn14, for which simultaneous TLR4 activation is required. Read More

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https://www.physiology.org/doi/10.1152/ajprenal.00627.2018
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http://dx.doi.org/10.1152/ajprenal.00627.2018DOI Listing
April 2019
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Inhibition of aerobic glycolysis enhances the anti-tumor efficacy of Zoptarelin Doxorubicin in triple-negative breast cancer cells.

J Obstet Gynaecol Res 2019 Apr 23. Epub 2019 Apr 23.

Department of Gynecology and Obstetrics, Georg-August-University, Göttingen, Germany.

Aim: A characteristic of cancer cells including triple-negative breast cancers (TNBC) is an increased aerobic glycolysis for ATP production representing a selective therapeutic target. More than 70% of TNBC express gonadotropin-releasing hormone receptors (GnRH-R). These receptors can be used for targeted chemotherapy with cytotoxic GnRH agonists such as Zoptarelin Doxorubicin, in which doxorubicin is covalently linked to [D-Lys ]GnRH. Read More

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http://dx.doi.org/10.1111/jog.13980DOI Listing

Moderate activation of Wnt/β-catenin signaling promotes the survival of rat nucleus pulposus cells via regulating apoptosis, autophagy, and senescence.

J Cell Biochem 2019 Apr 23. Epub 2019 Apr 23.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

This study aimed to investigate the specific role of Wnt/β-catenin signaling in compression-induced apoptosis, autophagy, and senescence in rat nucleus pulposus (NP) cells. Initially, the cells underwent various periods of exposure to 1.0 MPa compression. Read More

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http://dx.doi.org/10.1002/jcb.28518DOI Listing

Growth-promoting effects of low-level butyl benzyl phthalate exposure on human neuroblastoma SH-SY5Y cells.

J Appl Toxicol 2019 Apr 23. Epub 2019 Apr 23.

Department of Health Toxicology, MOE Key Laboratory of Environmental and Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.

The purpose of present study was to investigate the impact of butyl benzyl phthalate (BBP) on SH-SY5Y neuroblastoma cells in vitro. The cell counting kit-8 was used to measure cell proliferation and flow cytometry was utilized to study cell cycle phases and apoptosis. Western blotting and quantitative real-time polymerase chain reaction were used to detect levels of aromatase, estrogen receptors (ERs) and some apoptosis and cell cycle-related genes. Read More

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http://dx.doi.org/10.1002/jat.3803DOI Listing

Caffeic Acid Phenethyl Ester (CAPE) Protects PC12 Cells Against Cisplatin-Induced Neurotoxicity by Activating the AMPK/SIRT1, MAPK/Erk, and PI3k/Akt Signaling Pathways.

Neurotox Res 2019 Apr 23. Epub 2019 Apr 23.

Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Peripheral sensory neuropathy (PSN) is a well-known side effect of cisplatin characterized by axonal damage. In the early stage of neurotoxicity, cisplatin affects proteins that modulate neurite outgrowth and neuroplasticity, without inducing mitochondrial damage or apoptosis. There are no preventive therapies for cisplatin-induced peripheral neuropathy; therefore, measures to improve axonal growth and connectivity would be beneficial. Read More

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http://dx.doi.org/10.1007/s12640-019-00042-wDOI Listing

Measuring Apoptosis and Necrosis in Cholestatic Liver Injury.

Methods Mol Biol 2019 ;1981:133-147

Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS, USA.

Cholestasis can be induced by obstruction of bile ducts or intrahepatic toxicity of drugs and chemicals. However, the mode of cell death during cholestasis, i.e. Read More

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http://dx.doi.org/10.1007/978-1-4939-9420-5_9DOI Listing
January 2019

The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis.

Methods Mol Biol 2019 ;1981:117-132

Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

The blockage of bile flow, cholestasis, could lead to serious clinical outcomes, including severe liver injury. Accumulation of the cytotoxic molecules, such as bile acids, during cholestasis, not only impairs liver function, but also affects other organs, including the kidneys. Although the precise mechanisms of cytotoxicity and organ injury in cholestasis are far from clear, oxidative stress and its subsequent events seem to play a central role in this complication. Read More

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http://dx.doi.org/10.1007/978-1-4939-9420-5_8DOI Listing
January 2019
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A 3D Multiscale Model to Explore the Role of EGFR Overexpression in Tumourigenesis.

Bull Math Biol 2019 Apr 23. Epub 2019 Apr 23.

Division of Scientific Computing, Department of Information Technology, Uppsala University, 75105, Uppsala, Sweden.

The epidermal growth factor receptor (EGFR) signalling cascade is one of the main pathways that regulate the survival and division of mammalian cells. It is also one of the most altered transduction pathways in cancer. Acquired mutations in the EGFR/ERK pathway can cause the overexpression of EGFR on the surface of the cell, while others downregulate the inactivation of switched on intracellular proteins such as Ras and Raf. Read More

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http://dx.doi.org/10.1007/s11538-019-00607-yDOI Listing

Identification of microRNA transcriptome reveals that miR-100 is involved in the renewal of porcine intestinal epithelial cells.

Sci China Life Sci 2019 Apr 23. Epub 2019 Apr 23.

Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life Sciences, Hunan Normal University, Changsha, 410081, China.

MicroRNAs play important roles in various cellular processes, including differentiation, proliferation and survival. Using a pig model, this study sought to identify the miRNAs responsible for crypt-villus axis renewal of the small intestinal epithelium. Compared to the villus upper cells, there were 15 up-regulated and 41 down-regulated miRNAs in the crypt cells of the jejunum. Read More

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http://link.springer.com/10.1007/s11427-018-9338-9
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http://dx.doi.org/10.1007/s11427-018-9338-9DOI Listing
April 2019
1 Read

ASB3 knockdown promotes mitochondrial apoptosis via activating the interdependent cleavage of Beclin1 and caspase-8 in hepatocellular carcinoma.

Sci China Life Sci 2019 Apr 23. Epub 2019 Apr 23.

Department of Digestive Diseases of Huashan Hospital, Fudan University, Shanghai, 200040, China.

Apoptosis and autophagy are distinct cellular processes that can be highly interconnected. The cross talk between the two processes is indispensable in determining the overall cell fate. Although the apoptosis-promoting effect of caspases has been demonstrated, the roles of autophagy-related proteins and even autophagy itself in regulating apoptosis remain poorly understood. Read More

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http://dx.doi.org/10.1007/s11427-018-9505-0DOI Listing
April 2019
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HDAC-selective Inhibitor Cay10603 Has Single Anti-tumour Effect in Burkitt's Lymphoma Cells by Impeding the Cell Cycle.

Curr Med Sci 2019 Apr 23;39(2):228-236. Epub 2019 Apr 23.

Medical Research Center, Shengjing Hospital, China Medical University, Shenyang, 110004, China.

Histone deacetylases (HDACs) inhibitors are novel in cancer therapy nowadays. HDAC6-selective inhibitors exert advantageous effects due to higher selectivity and less toxicity. We explored the anti-tumor effect and the molecular mechanism of cay10603, a potent HDAC6 inhibitor in Burkitt's lymphoma cells. Read More

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http://dx.doi.org/10.1007/s11596-019-2024-4DOI Listing

Notoginseng Saponin Rg1 Prevents Cognitive Impairment through Modulating APP Processing in Aβ-injected Rats.

Curr Med Sci 2019 Apr 23;39(2):196-203. Epub 2019 Apr 23.

Cell Molecular Biology Laboratory of Basic Medical College, Hubei University of Chinese Medicine, Wuhan, 430065, China.

With the intensification of the aging process of the world, Alzheimer's disease (AD), which is the main type of senile dementia, has become a primary problem in the present society. Lots of strategies have been used to prevent and treat AD in animal models and clinical trials, but most of them ended in failure. Panax notoginseng saponins (PNS) contain a variety of monomer compositions which have been separated and identified. Read More

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http://dx.doi.org/10.1007/s11596-019-2019-1DOI Listing

The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein, Induce Apoptosis in HEp-2 and Vero Cells.

Virol Sin 2019 Apr 23. Epub 2019 Apr 23.

Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.

Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of SAFV into HEp-2 and Vero cells to assess their ability to induce apoptosis, and found that the 2B and 3C proteins are proapoptotic. Read More

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http://dx.doi.org/10.1007/s12250-019-00116-1DOI Listing

Reduced ambient temperature exacerbates SIRS-induced cardiac autonomic dysregulation and myocardial dysfunction in mice.

Basic Res Cardiol 2019 Apr 23;114(3):26. Epub 2019 Apr 23.

Institute of Molecular Cell Biology, Jena University Hospital, Friedrich Schiller University, Hans-Knöll-Straße 2, 07745, Jena, Germany.

Sepsis-induced myocardial depression (SIMD) is an early and frequent consequence of the infection-induced systemic inflammatory response syndrome. In homiotherms, variations in ambient temperature (T) outside the thermoneutral zone induce thermoregulatory responses mainly driven by a gradually increased sympathetic activity, which may affect disease severity. We hypothesized that thermoregulatory responses upon reduced T exposition aggravate SIMD in mice. Read More

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http://dx.doi.org/10.1007/s00395-019-0734-1DOI Listing

Cellular responses of BRCA1-defective HCC1937 breast cancer cells induced by the antimetastasis ruthenium(II) arene compound RAPTA-T.

Apoptosis 2019 Apr 23. Epub 2019 Apr 23.

Laboratory of Pharmaceutical Biotechnology, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand.

An organometallic ruthenium(II) arene compound, Ru(η-toluene)(PTA)Cl (PTA = 1,3,5-triaza-7-phosphaadamantane), termed RAPTA-T, exerts promising antimetastatic properties. In this study, the effects of RAPTA-T on BRCA1-defective HCC1937 breast cancer cells have been investigated, and compared to its effects on BRCA1-competent MCF-7 breast cancer cells. RAPTA-T showed a very low cytotoxicity against both tested cells. Read More

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http://dx.doi.org/10.1007/s10495-019-01544-wDOI Listing
April 2019
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Enhanced Proapoptotic Effects of Water Dispersed Complexes of 4-Thiazolidinone-Based Chemotherapeutics with a PEG-Containing Polymeric Nanocarrier.

Nanoscale Res Lett 2019 Apr 23;14(1):140. Epub 2019 Apr 23.

International Research and Innovation in Medicine Program, Cedars-Sinai Medical Center, Los Angeles, CA, 90048-5502, USA.

Aim: To study whether water formulation of the complex of 4-thiazolidinone derivatives with a PEG-containing polymeric nanocarrier enhances their pro-apoptotic action towards rat glioma C6 cells.

Methods: Mechanisms of antineoplastic effects of 4-thiazolidinone derivatives were investigated in vitro with rat glioma C6 cells. Cell nativity, cell cycling pattern, and Annexin V expression were evaluated and DNA damage was estimated by DNA comet analysis. Read More

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http://dx.doi.org/10.1186/s11671-019-2945-7DOI Listing

Vitamin E and cancer: an update on the emerging role of γ and δ tocotrienols.

Eur J Nutr 2019 Apr 16. Epub 2019 Apr 16.

Department of Basic and Clinical Sciences, University of Nicosia Medical School, 21 Ilia Papakyriakou, 2414 Engomi, P.O. Box 24005, 1700, Nicosia, Cyprus.

Despite significant advances in the diagnosis and treatment of cancer, the latter still remains a fatal disease due to the lack of prevention, early diagnosis, and effective drugs. Radiotherapy, chemotherapy, and surgery are not only expensive but produce a number of side effects that are detrimental to the patients' quality of life. Therefore, there is a great need to discover anti-cancer therapies that are specific to cancer cells and affordable, safe, and well tolerated by the patients. Read More

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http://dx.doi.org/10.1007/s00394-019-01962-1DOI Listing

TPP1 OB-fold domain protein suppresses cell proliferation and induces cell apoptosis by inhibiting telomerase recruitment to telomeres in human lung cancer cells.

J Cancer Res Clin Oncol 2019 Apr 23. Epub 2019 Apr 23.

Department of Lung Cancer, Tianjin Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Huan-Hu-Xi Road, Ti-Yuan-Bei, He Xi District, Tianjin, 300060, China.

Purpose: Maintaining telomeres by recruiting telomerase-to-chromosome ends is essential for cancer cell survival. Inhibiting telomerase recruitment to telomeres represents a novel strategy for telomere-based lung cancer therapy. However, approaches for interrupting telomerase recruitment for cancer therapy still need to be explored. Read More

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http://dx.doi.org/10.1007/s00432-019-02921-3DOI Listing

Continuous cuprizone intoxication allows active experimental autoimmune encephalomyelitis induction in C57BL/6 mice.

Histochem Cell Biol 2019 Apr 23. Epub 2019 Apr 23.

Institute of Anatomy, Rostock University Medical Center, Gertrudenstrasse 9, 18057, Rostock, Germany.

Oligodendrocyte degeneration is a hallmark of multiple sclerosis pathology, and protecting oligodendrocytes and myelin is likely to be of clinical relevance. Traditionally, oligodendrocyte and myelin degeneration are viewed as a direct consequence of an inflammatory attack, but metabolic defects might be equally important. Appropriate animal models to study the interplay of inflammation and metabolic injury are, therefore, needed. Read More

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http://dx.doi.org/10.1007/s00418-019-01786-4DOI Listing

Connexin 43 hemichannels protect bone loss during estrogen deficiency.

Bone Res 2019 22;7:11. Epub 2019 Apr 22.

Department of Biochemistry and Structural Biology, UT Health San Antonio, San Antonio, TX 78229 USA.

Estrogen deficiency in postmenopausal women is a major cause of bone loss, resulting in osteopenia, osteoporosis, and a high risk for bone fracture. Connexin 43 (Cx43) hemichannels (HCs) in osteocytes play an important role in osteocyte viability, bone formation, and remodeling. We showed here that estrogen deficiency reduced Cx43 expression and HC function. Read More

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http://dx.doi.org/10.1038/s41413-019-0050-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476886PMC

miR-4521-FAM129A axial regulation on ccRCC progression through TIMP-1/MMP2/MMP9 and MDM2/p53/Bcl2/Bax pathways.

Cell Death Discov 2019 15;5:89. Epub 2019 Apr 15.

1Department of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, 116044 Dalian, China.

Clear cell renal cell carcinoma (ccRCC) is the most aggressive RCC subtype with high metastasis, chemotherapy and radiotherapy resistance, and poor prognosis. This study attempted to establish the deregulations of miR-4521 and FAM129A together with their correlation to and mechanism of regulation of ccRCC development and progression. FAM129A acted as tumor promotor and miR-4521 acted as a suppressor in ccRCC. Read More

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http://dx.doi.org/10.1038/s41420-019-0167-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465337PMC