4,470 results match your criteria Apmis[Journal]

Biomarkers for iron metabolism among patients hospitalized with community-acquired pneumonia caused by infection with SARS-CoV-2, bacteria, and influenza.

APMIS 2022 Jun 25. Epub 2022 Jun 25.

Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.

Background: Ferritin, the central iron storage protein, has attracted attention as a biomarker of severe COVID-19. Few studies have investigated regulators of iron metabolism in the context of COVID-19. The aim was to evaluate biomarkers for iron metabolism in the acute phase response to community-acquired pneumonia (CAP) caused by SARS-CoV-2 compared to CAP caused by bacteria or influenza virus in hospitalized patients. Read More

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A novel fully human recombinant antibody neutralizing the α-hemolysin of Staphylococcus aureus.

APMIS 2022 Jun 25. Epub 2022 Jun 25.

Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China.

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to almost all β-lactam antibiotics. Hence, new ways to control MRSA infection, such as antibacterial antibodies, need to be explored. α-hemolysin is the most important virulence factor widely expressed in S. Read More

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Is there a role of epigenetics in gut microbiome and neurological disorders?

APMIS 2022 Jun 22. Epub 2022 Jun 22.

Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah, UAE.

Cross-talk between gut microbiota and the nervous system in the pathophysiology of neurological diseases has become a recent focus of interest. The underlying mechanisms are complex, and we suggest that they might involve gut bacterial metabolite-induced epigenetic modulations of neurodegenerative disorders. Advances in epigenetic techniques could provide the key to understanding the epigenetics of the gut-brain axis. Read More

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Differences in gene expression despite identical histomorphology in sinonasal intestinal-type adenocarcinoma and metastases from colorectal adenocarcinoma.

APMIS 2022 Jun 3. Epub 2022 Jun 3.

Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Copenhagen University Hospital, Copenhagen, Denmark.

Sinonasal intestinal-type adenocarcinoma (sITAC) is histomorphologically indistinguishable from colorectal adenocarcinoma (CRC) leading to diagnostic challenges. Metastases from CRCs to the sinonasal tract have been reported. The aim of the study was to identify a biomarker making it possible to distinguish between sITAC and metastases of colorectal origin. Read More

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Mutation of KRAS in colorectal adenocarcinoma in Greenland.

APMIS 2022 Jun 2. Epub 2022 Jun 2.

Department of Pathology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

KRAS mutation is one of the most frequent driver mutations in colorectal cancer (CRC) and is also a prognostic biomarker. The aim of the present study was to determine the frequency of KRAS mutations over time in the Greenlandic population diagnosed with CRC. In total, 578 patients with the diagnosis of adenocarcinoma between 1988 and 2017 were identified. Read More

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Inter-observer agreement of tumor infiltrating lymphocytes in primary HER2-positive breast cancer and correlation between tissue microarray and full tumor-sections.

APMIS 2022 May 31. Epub 2022 May 31.

Department of Surgical Pathology, Zealand University Hospital, Roskilde, Denmark.

Tumor infiltrating lymphocytes (TILs) have predictive and prognostic potential in HER2-positive breast cancer (HER2 + BC). Due to tumor heterogeneity, guidelines recommend evaluation on full tumor-sections over biopsies, but aren't clear regarding tissue microarrays (TMAs). Herein, we investigate the inter-observer agreement of TILs assessment in HER2 + BC on full-sections and TMAs using a standardized method. Read More

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Increased sputum lactate during oral glucose tolerance test in cystic fibrosis.

APMIS 2022 May 30. Epub 2022 May 30.

Cystic Fibrosis Centre Copenhagen, Rigshospitalet, Copenhagen, Denmark.

Blood glucose levels exceeding 8 mM are shown to increase glucose levels in airway surface in cystic fibrosis (CF). Moreover, high levels of endobronchial glucose are proposed to increase the growth of common CF bacteria and feed the neutrophil-driven inflammation. In the infected airways, glucose may be metabolized by glycolysis to lactate by both bacteria and neutrophils. Read More

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Short communication: dog bite and back pain.

APMIS 2022 May 27. Epub 2022 May 27.

University Hospital of Lille (CHU Lille), Lille, France.

Capnocytophaga canimorsus is a Gram-negative rod commensal of oral cavity of dogs and cats. It can cause sepsis, septic shock, endocarditis, and meningitis associated with bites or licking wounds, especially in immunocompromised patients. Herein, we report a case of C. Read More

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PD-L1 expression and FGFR-mutations among Danish patients diagnosed with metastatic urothelial carcinoma: A retrospective and descriptive study.

APMIS 2022 May 26. Epub 2022 May 26.

Department of Oncology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

Checkpoint inhibitors have changed the treatment landscape of advanced urothelial carcinoma (mUC), and recently, a fibroblast-growth-factor-receptor (FGFR) inhibitor has been introduced. This study aimed at estimating programmed death-ligand 1 (PD-L1) expression in primary tumors (PTs) and the PD-L1 expression concordance between PTs and paired metastases in 100 patients with UC managed in the real-world setting. Further, the aim was to investigate FGFR1-3 aberrations and the correlation between FGFR1-3 aberrations and PD-L1 expression. Read More

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Transcriptomic fingerprint of bacterial infection in lower extremity ulcers.

APMIS 2022 May 14. Epub 2022 May 14.

Department of Immunology and Microbiology, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark.

Clinicians and researchers utilize subjective, clinical classification systems to stratify lower extremity ulcer infections for treatment and research. The purpose of this study was to examine whether these clinical classifications are reflected in the ulcer's transcriptome. RNA sequencing (RNA-seq) was performed on biopsies from clinically infected lower extremity ulcers (n = 44). Read More

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Performance of PCR-based syndromic testing compared to bacterial culture in patients with suspected pneumonia applying microscopy for quality assessment.

APMIS 2022 Jul 22;130(7):417-426. Epub 2022 May 22.

Department of Clinical Microbiology, Copenhagen University Hospital - Amager and Hvidovre, Copenhagen, Denmark.

Syndromic testing for lower respiratory tract infections with BioFire® FilmArray® Pneumonia Panel Plus (BF) detects 27 pathogens with a turn-around-time of one hour. We compared the performance of BF with culture. Samples from 298 hospitalized patients with suspected pneumonia routinely sent for culture were also analyzed using BF. Read More

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Injection site microflora in persons with diabetes: why needle reuse is not associated with increased infections?

APMIS 2022 Jul 17;130(7):404-416. Epub 2022 May 17.

Department of Immunology and Microbiology, Copenhagen University, Copenhagen, Denmark.

Needle reuse is a common practice and primary cause of customer compliance issues such as pain, bruising, clogging, injection site reactions (ISR), and associated lipodystrophy. This study aimed to characterize skin microflora at injection sites and establish microbial contamination of used pen injectors and needles. The second objective was to evaluate the risk of infections during typical and repeated subcutaneous injections. Read More

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Dynamics of a Staphylococcus aureus infective endocarditis simulation model.

APMIS 2022 Apr 23. Epub 2022 Apr 23.

Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.

Infective endocarditis (IE) is a serious infection of the inner surface of heart, resulting from minor lesions in the endocardium. The damage induces a healing reaction, which leads to recruitment of fibrin and immune cells. This sterile healing vegetation can be colonized during temporary bacteremia, inducing IE. Read More

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Aspergilloma complicating previous COVID-19 pneumonitis - a case report.

APMIS 2022 Jul 16;130(7):397-403. Epub 2022 May 16.

Department of Internal Medicine, Respiratory Medicine Section, Herlev and Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.

Aspergillomas are found in pre-existing cavities in pulmonary parenchyma. To the best of our knowledge, aspergilloma has not previously been reported in COVID-19-associated pulmonary architecture distortion combined with barotrauma from invasive mechanical ventilation therapy. We present a case of a 67-year-old woman, who suffered from severe COVID-19 in the summer of 2020 with no suspicion of infection with Aspergillus in the acute phase. Read More

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Murine burn lesion model for studying acute and chronic wound infections.

APMIS 2022 Jul 16;130(7):477-490. Epub 2022 May 16.

Department of Clinical Microbiology, Copenhagen University Hospital, Copenhagen, Denmark.

Acute wounds, such as thermal injury, and chronic wounds are challenging for patients and the healthcare system around the world. Thermal injury of considerable size induces immunosuppression, which renders the patient susceptible to wound infections, but also in other foci like the airways and urinary tract. Infected thermal lesions can progress to chronic wounds with biofilm making them more difficult to treat. Read More

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Acinetobacter baumannii: insights towards a comprehensive approach for the prevention of outbreaks in health-care facilities.

APMIS 2022 Jun 25;130(6):330-337. Epub 2022 Apr 25.

Division of Microbiology, Department of Pathology, Faculty of Medicine, University of Porto, Porto, Portugal.

Acinetobacter baumannii is known to be an opportunistic pathogen frequently responsible for outbreaks in health-care facilities, particularly in Intensive Care Units (ICU). It can easily survive in the hospital setting for long periods and can be transmitted throughout the hospital in a variety of ways, explored in this review. It can also easily acquire antibiotic resistance determinants rendering several antibiotic drugs useless. Read More

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A metatranscriptomic approach to explore longitudinal tissue specimens from non-healing diabetes related foot ulcers.

APMIS 2022 Jul 10;130(7):383-396. Epub 2022 May 10.

South West Sydney Limb Preservation and Wound Research, South Western Sydney LHD, Sydney, NSW, Australia.

Cellular mechanisms and/or microbiological interactions which contribute to chronic diabetes related foot ulcers (DRFUs) were explored using serially collected tissue specimens from chronic DRFUs and control healthy foot skin. Total RNA was isolated for next-generation sequencing. We found differentially expressed genes (DEGs) and enriched hallmark gene ontology biological processes upregulated in chronic DRFUs which primarily functioned in the host immune response including: (i) Inflammatory response; (ii) TNF signalling via NFKB; (iii) IL6 JAK-STAT3 signalling; (iv) IL2 STAT5 signalling and (v) Reactive oxygen species. Read More

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The human microbiome in disease and pathology.

Jim Manos

APMIS 2022 Apr 8. Epub 2022 Apr 8.

Infection, Immunity and Inflammation, School of Medical Sciences, Faculty of Medicine and Health, The Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.

This narrative review seeks to examine the relationships between bacterial microbiomes and infectious disease. This is achieved by detailing how different human host microbiomes develop and function, from the earliest infant acquisitions of maternal and environmental species through to the full development of microbiomes by adulthood. Communication between bacterial species or communities of species within and outside of the microbiome is a factor in both maintenance of homeostasis and management of threats from the external environment. Read More

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Endotracheal lactate reflects lower respiratory tract infections and inflammation in intubated patients.

APMIS 2022 Mar 29. Epub 2022 Mar 29.

Department of Anesthesiology and Intensive Care, Nordsjaellands Hospital, Copenhagen University Hospital, Hillerød, Denmark.

The aim of this study was to assess L-lactate and D-lactate in endotracheal aspirate from intubated patients hospitalized at the intensive care unit and explore their use as diagnostic biomarkers for inflammation and lower respiratory tract infections (LRTI). Tracheal aspirates from 91 intubated patients were obtained at time of intubation and sent for microbiological analyses, neutrophil count, and colorimetric lactate measurements. We compared the concentration of lactate from patients with microbiological verified LRTI or clinical/radiological suspicion of LRTI with a control group. Read More

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Expression of EPHA5 in lung adenocarcinoma is associated with lymph node metastasis and EGFR mutation.

APMIS 2022 Jun 4;130(6):338-345. Epub 2022 Apr 4.

Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.

EPHA5 is a member of the Eph family of tyrosine kinase receptors, which affect carcinogenesis. The expression level of the EPHA5 receptor in a set of lung adenocarcinoma tissue samples was checked using immunohistochemistry. The relationship between EPHA5 expression and clinicopathological parameters, and epidermal growth factor receptor (EGFR) and Braf mutations were analyzed. Read More

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PD-L1 immunohistochemistry: Clones, cutoffs, and controversies.

APMIS 2022 Jun 3;130(6):295-313. Epub 2022 Apr 3.

James Homer Wright Pathology Laboratories, Massachusetts General Hospital, Boston, MA, USA.

Cancer immunotherapy has become a major component of oncologic treatment for a growing number of malignancies. Of particular interest to pathology has been monoclonal antibody therapy targeting immune checkpoints, notably programmed cell death (PD-1) and programmed cell death ligand (PD-L1). Targeting of these checkpoints attempt to overcome tumor evasion of the immune system. Read More

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Role of IL-22 in homeostasis and diseases of the skin.

APMIS 2022 Jun 6;130(6):314-322. Epub 2022 Apr 6.

The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Interleukin-22 (IL-22) is a cytokine mainly produced by T cells and innate lymphoid cells (ILC). IL-22 primarily targets non-hematopoietic cells such as epithelial cells and fibroblasts. In the skin, IL-22 promotes the proliferation of keratinocytes and dermal fibroblasts. Read More

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Peritumoral histopathologic findings in patients with chronic viral hepatitis-associated hepatocellular carcinoma.

APMIS 2022 Jun 18;130(6):346-356. Epub 2022 Apr 18.

Department of Molecular Pathology, Graduate School of Health Sciences, Dokuz Eylul University, Izmir, Turkey.

Data on peritumoral histopathologic findings in patients with hepatocellular carcinoma (HCC) is limited. In this retrospective study, we evaluated the peritumoral histopathologic changes in patients with chronic viral hepatitis (CVH)-associated HCC (CVH-HCC) and their prognostic value. 61 consecutive cirrhotic patients who underwent liver transplantation due to CVH-HCC were included. Read More

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BUB1 predicts poor prognosis and immune status in liver hepatocellular carcinoma.

APMIS 2022 Jul 17;130(7):371-382. Epub 2022 May 17.

Department of Oncology Surgery, Lanzhou University Second Hospital, Lanzhou, China.

Accurate assessment of the tumour immune microenvironment promotes individualized immunotherapy regimens and screens dominant populations suitable for immunotherapy. Therefore, potential molecular markers were investigated to make an overall assessment of the immune microenvironment status of liver hepatocellular carcinoma (LIHC). In this study, a total of 121 differentially expressed genes (DEGs) were identified, and DEGs were enriched in the epithelial-mesenchymal transition, hypoxia, myogenesis, and p53 pathways. Read More

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The use of core genome multilocus sequence typing to determine the duration of vancomycin-resistant Enterococcus faecium outbreaks.

APMIS 2022 Jun 31;130(6):323-329. Epub 2022 Mar 31.

Department of Clinical Microbiology, Copenhagen University Hospital - Amager and Hvidovre, Copenhagen, Denmark.

The prevalence of vancomycin-resistant Enterococcus faecium has increased rapidly, and in Denmark, we are facing an endemic outbreak situation in hospitals. The aim of this study was to use whole-genome sequencing (WGS) and core genome multilocus sequencing typing (cgMLST) to determine the duration of VREfm outbreaks and thereby evaluate the effect of our infection control strategies. We included all VREfm isolates from six hospitals in the Capital Region of Denmark that were sequenced between 2012 and 2020. Read More

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PD-L1 and PD-1 expression in thyroid follicular epithelial dysplasia: Hashimoto thyroiditis related atypia and potential papillary carcinoma precursor.

APMIS 2022 May 13;130(5):276-283. Epub 2022 Mar 13.

Pathology, Fimlab Laboratories, Tampere, Finland.

Programmed cell death ligand (PD-L1)/PD-1 expression has been studied in a variety of cancers and blockage of PD-L1/PD-1 pathway is a cornerstone of immunotherapy. We studied PD-L1/PD-1 immunohistochemical expression in 47 thyroid gland specimens in groups of (1) Hashimoto thyroiditis (HT) only; (2) HT and follicular epithelial dysplasia (FED); and (3) HT, FED, and papillary thyroid carcinoma (PTC). PD-1 positivity was found in immune cells, namely in lymphocytes, macrophages, and plasma cells with mean values for lymphocytes and macrophages 9% in HT group, 4% in FED group, and 4% in PTC group. Read More

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Native aortic valve Staphylococcus warneri endocarditis after COVID-19 infection: a case report and a review of literature.

APMIS 2022 May 11;130(5):270-275. Epub 2022 Mar 11.

Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

We report a case of Staphylococcus warneri native valve endocarditis in an immunocompetent healthy adult, without known risk factors for infective endocarditis, two months following COVID-19 infection, who recovered with conservative treatment. Additionally, we reviewed previous cases of native valve endocarditis caused by Staphylococcus warneri and summarized the main clinical implications. Read More

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Study of humoral and cellular immunity in vaccinated with mRNA-1273.

APMIS 2022 May 3;130(5):261-269. Epub 2022 Mar 3.

Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain.

The new vaccines against SARS-CoV-2 have raised a lot of expectations about their ability to induce immunity and the duration of this. This is the case of mRNA vaccines such as Moderna's mRNA-1273. Therefore, it is necessary to study the humoral and cellular immunity generated by these vaccines. Read More

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Association of sickle cell trait with β-cell dysfunction and physical activity in adults living with and without HIV in Tanzania.

APMIS 2022 Apr 1;130(4):230-239. Epub 2022 Mar 1.

Mwanza Research Centre, National Institute for Medical Research, Mwanza, Tanzania.

This study aimed to investigate sickle cell trait (SCT) associations with physical activity, markers of insulin secretion and resistance, and glucose among people living with HIV infection (PLWH), both antiretroviral therapy (ART) naive and experienced, and HIV-uninfected adults. This was a cross-sectional study conducted in Mwanza, Northwestern Tanzania. We used data of 668 participants attained from two sub-studies of CICADA study. Read More

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APMIS 2022 Mar 9;130(3):193. Epub 2022 Feb 9.

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