4,388 results match your criteria Apmis[Journal]


Animal models of chronic and recurrent Pseudomonas aeruginosa lung infection - significance of macrolide treatment.

APMIS 2021 Jun 12. Epub 2021 Jun 12.

Department of Clinical Microbiology, Rigshospitalet, Copenhagen University Hospital. Copenhagen, Copenhagen, Denmark.

Animal models of human diseases are invaluable and inevitable elements in identifying and testing novel treatments of serious diseases, including severe infections. Planning and conducting investigator initiated human trials are generally accepted as being enormously challenging. In contrast, it is often underestimated how much planning, including background and modifying experiments, there is needed to establish a relevant infectious disease animal model. Read More

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The Effect of Gamma and Microwave Radiation Sterilization on Periodontological Grafts for Microbiological Evaluation.

APMIS 2021 Jun 12. Epub 2021 Jun 12.

Hacettepe University, Faculty of Dentistry, Department of Periodontology, 06100, Sıhhiye Ankara, Turkey.

Purpose: Periodontological grafts are materials used in dentistry to regenerate lost gingival soft tissues or bone parts. In the case of direct contact with blood, the possibility of disease transmission from the source to the patient is high. This source can be an animal or a human. Read More

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Reliability of Ki67 visual scoring app compared to eyeball estimate and digital image analysis and its prognostic significance in hormone receptor positive breast cancer.

APMIS 2021 May 30. Epub 2021 May 30.

University of British Columbia, Vancouver, British Columbia, Canada.

Background: We analysed the reproducibility of Ki67 labelling index (LI) between two scorers using the International Ki67 Working Group (IKWG) global methods on an Android application (APP), correlated the APP and eyeball estimate (EBE) with digital image analysis (DIA) scores and determined the prognostic significance of Ki67LI.

Methods: Global weighted (GW) and global unweighted (GUW)) Ki67 app scores of hormone receptor positive and HER2 (human epidermal growth factor receptor 2) negative breast cancer patients were obtained. Reproducibility of Ki67LI between 2 scorers and correlation of APP and EBE scores with DIA scores was performed. Read More

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Status: nosocomial transmission and prevention of SARS-CoV-2 in a Danish context.

APMIS 2021 May 29. Epub 2021 May 29.

Department of Clinical Microbiology, Hvidovre Hospital, Copenhagen University, Copenhagen, Denmark.

The unexpected pandemic with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has challenged the health care sector as regards preventing and controlling the virus from spreading between patients and hospital personnel. The massive spread of the pandemic has led State authorities to introduce restrictions on society and public behavior unprecedented in modern times. First, we describe the Danish effort regarding standard precautions, personal protective equipment, and disinfection in the health care setting with Denmark as an example. Read More

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Malignant Peripheral Nerve Sheath Tumors Arising from Schwannomas: Case Series and Literature Review.

APMIS 2021 May 29. Epub 2021 May 29.

Departments of Orthopaedics, Massachusetts General Hospital, Boston, USA.

Background: A malignant peripheral nerve sheath tumor (MPNST) arising from a schwannoma is extremely rare, with limited literature on its clinicopathologic features. Here, we present a case series and literature review on patients with MPNSTs arising from schwannomas.

Methods: We performed a retrospective review of patients from our institution's records to identify those with MPNSTs arising from schwannomas. Read More

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Sclerosing Epithelioid Fibrosarcoma: In-depth review of a genetically heterogeneous tumor.

APMIS 2021 May 28. Epub 2021 May 28.

Department of Laboratory Medicine & Pathology, Hamad Medical Corporation, Doha, State of Qatar.

First described in 1995 by Meis-Kindbloom et al as a variant of fibrosarcoma simulating carcinoma, sclerosing epithelioid fibrosarcoma (SEF) is a malignant soft tissue sarcoma characterized by epithelioid cells in dense sclerotic stroma, frequent immunoreactivity for MUC4 and heterogeneous genetic profile with recurrent EWSR1 gene rearrangement. It typically affects middle age adults with a predilection for the lower extremity. It is believed that SEF is closely related to low-grade fibromyxoid sarcoma (LGFMS), both tumors show overlapping features in morphology, immunophenotype and molecular profile. Read More

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Vitamin A deficiency in K14E7HPV expressing transgenic mice facilitates the formation of malignant cervical lesions.

APMIS 2021 May 28. Epub 2021 May 28.

Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, Mexico.

Rodolfo Ocadiz-Delgado, Nicolás Serafin-Higuera, Elizabeth Alvarez-Rios, Enrique García-Villa, Manuel Tinajero-Rodríguez, Genaro Rodríguez-Uribe, Derly Constanza Escobar-Wilches, Marta Estela Albino-Sánchez, Alejandro Ramírez-Rosas, Adolfo Sierra-Santoyo, Rogelio Hernández-Pando, Paul Lambert and Patricio Gariglio. Vitamin A deficiency in K14E7HPV expressing transgenic mice facilitates the formation of malignant cervical lesions. Infection with high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer (CC), but viral infection alone does not guarantee the development of this malignancy. Read More

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Antizyme inhibitor 2 (AZIN2) associates with better prognosis of head and neck minor salivary gland adenoid cystic carcinoma.

APMIS 2021 May 28. Epub 2021 May 28.

Research Programs Unit, Translational Cancer Biology Program, University of Helsinki, Helsinki, Finland.

Aim: The key regulator of the polyamine biosynthetic pathway is ornithine decarboxylase (ODC). ODC is activated by antizyme inhibitor 1 (AZIN1) and 2 (AZIN2). AZIN1 and recently AZIN2 have been related to cancer, however, their functions in adenoid cystic carcinoma (ACC) have not been studied. Read More

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Extensive microbiological respiratory tract specimen characterization in critically ill COVID-19 patients.

APMIS 2021 May 5. Epub 2021 May 5.

The Regional Department of Clinical Microbiology, Zealand University Hospital, Roskilde, Denmark.

Microbial co-infections may contribute to the pulmonary deterioration in COVID-19 patients needing intensive care treatment. The present study portrays the extent of co-infections in COVID-19 ICU patients. Conventional culture, molecular detections for atypical aetiologies, QiaStat-Dx® respiratory panel V2 detecting 21 respiratory pathogens and ribosomal DNA genes 16S/18S amplicon-based microbiome analyses were performed on respiratory samples from 34 COVID-19 patients admitted to the ICU. Read More

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Characterization of a lytic EBP bacteriophage with large size genome against Enterobacter cloacae.

APMIS 2021 May 5. Epub 2021 May 5.

Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan, 54590, Pakistan.

Enterobacter cloacae (E. cloacae) is an emerging nosocomial pathogen that had acquired antibiotic resistance against multiple classes of antibiotics. The current study was aimed to isolate and characterize lytic bacteriophage against E. Read More

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Toll-like receptor 5 and 8 in hepatocellular carcinoma.

APMIS 2021 May 5. Epub 2021 May 5.

Surgery Research Unit, Medical Research Center Oulu, Cancer and Translational Medicine Research Unit, University of Oulu and Oulu University Hospital, Oulu, Finland.

Toll-like receptors (TLRs) are components of innate immunity, but also have a role in carcinogenesis. The prognostic value of TLR5 and TLR8 tumor expression was examined in contrast with known risk markers Ki67 and p53. All HCC patients from Oulu University Hospital with available representative tumor sample were included in this study (n = 182). Read More

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Electronic reporting of diagnostic laboratory test results from all healthcare sectors is a cornerstone of national preparedness and control of COVID-19 in Denmark.

APMIS 2021 May 4. Epub 2021 May 4.

Data Integration and Analysis, Division of Infection Preparedness, Statens Serum Institut, Copenhagen S, Denmark.

The COVID-19 pandemic has led to an unprecedented demand for real-time surveillance data in order to inform critical decision makers regarding the management of the pandemic. The aim of this review was to describe how the Danish national microbiology database, MiBa, served as a cornerstone for providing data to the real-time surveillance system by linkage to other nationwide health registries. The surveillance system was established on an existing IT health infrastructure and a close network between clinical microbiologists, information technology experts, and public health officials. Read More

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Surgically Induced Ischemia has no Impact on Protein Expression Levels of HIF-1α and Related Biomarkers in Renal Cell Carcinoma.

APMIS 2021 May 4. Epub 2021 May 4.

Department of Pathology, Odense University Hospital, Odense, Denmark.

Background And Purpose: The increasing demands for personalized targeted therapy directed against Renal Cell Carcinoma has driven a search for predictive markers. Novel therapies targeting HIF-1α in Renal Cell Carcinoma have been developed and HIF-1α has been suggested as a novel predictive marker of response to therapy. The surgical resection of a kidney tumor induces tissue ischemia and HIF-1α is an oxygen-sensitive transcription factor, which is known to be up-regulated during hypoxia. Read More

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The COVID-19 pandemic: Key considerations for the epidemic and its control.

APMIS 2021 May 1. Epub 2021 May 1.

Department of science and environment, Roskilde University, Roskilde, Denmark.

The response to the ongoing COVID-19 pandemic has been characterized by draconian measures and far too many important unknowns, such as the true mortality risk, the role of children as transmitters and the development and duration of immunity in the population. More than a year into the pandemic much has been learned and insights into this novel type of pandemic and options for control are shaping up. Using a historical lens, we review what we know and still do not know about the ongoing COVID-19 pandemic. Read More

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Corrigendum.

Authors:

APMIS 2021 May 25;129(5):280. Epub 2021 Mar 25.

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Pentraxin-3 detected in human saliva shows limited correlation with biomarkers associated with systemic inflammation.

APMIS 2021 Jun 7;129(6):304-313. Epub 2021 May 7.

Division of Inflammation and Infection, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.

Pentraxin-3 (PTX3) is a conserved protein of the innate immune system which has been less studied than the pentraxin C-reactive protein (CRP), but it is of relevance in, for example, vascular pathology and pregnancy morbidities. Since the interest in salivary biomarkers in general is increasing, we asked whether PTX3 could be detected in saliva and if any substantial diurnal variation occurs. In addition, we evaluated association with biomarkers of systemic inflammation (interleukin (IL)-1β, IL-6, and IL-8 and CRP), body mass index (BMI), smoking, and age. Read More

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Beta-hemolytic streptococci A, C, and G are susceptible to cloxacillin.

APMIS 2021 Jun 29;129(6):314-316. Epub 2021 Apr 29.

Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark.

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Epidemics before microbiology: stories from the plague in 1711 and cholera in 1853 in Copenhagen.

Authors:
Adam Bencard

APMIS 2021 Apr 2. Epub 2021 Apr 2.

Medical Museion and NNF Center for Basic Metabolic Research, University of Copenhagen, København, Denmark.

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Reduced frequency of T helper 17 and T helper 1 cells and their association with critical coronavirus disease 2019.

APMIS 2021 May 31;129(5):271-279. Epub 2021 Mar 31.

Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

There is very little knowledge about the immune responses, particularly cellular immunity to coronavirus disease 2019 (COVID-19). The main objective of this study was to evaluate the frequency of T helper (Th) cell subtypes, including Th1, Th17, and Treg cells, in moderate-to-severe and critical COVID-19 patients compared to healthy controls. Twenty-nine moderate-to-severe and 13 critical patients confirmed for COVID-19, and 15 healthy subjects were included in this study. Read More

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Stanford parameters stratify the risk of recurrence in gynecologic smooth muscle tumors of uncertain malignant potential.

APMIS 2021 Jun 13;129(6):283-290. Epub 2021 Apr 13.

Anatomic Pathology Unit, Department of Advanced Biomedical Sciences, School of Medicine, University of Naples Federico II, Naples, Italy.

Smooth muscle tumor of uncertain malignant potential (STUMP) is an ill-defined category of neoplasms, which represent a diagnostic challenge. We aimed to assess whether the Stanford parameters, that is, high mitotic index (≥10/10HPF), significant atypia (moderate-to-severe), and coagulative tumor cell necrosis (CTCN), even when focal or ambiguous, may be used to stratify the risk of recurrence in gynecological smooth muscle tumor of uncertain malignant potential (STUMP). Electronic databases were searched from their inception to October 2019. Read More

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The Hammer vs Mitigation-A comparative retrospective register study of the Swedish and Danish national responses to the COVID-19 pandemic in 2020.

APMIS 2021 Mar 23. Epub 2021 Mar 23.

Department of Infectious Diseases, The Heartcentre, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

In the efforts to dampen the COVID-19 pandemic, governments are compelled to outweigh disease control efforts to the possible negative consequences of closing large parts of society. Although Denmark and Sweden are alike in political organization and health care, national responses to the 2020 COVID-19 epidemic differed noticeably. Denmark initiated a hard lock down followed by an outbreak control strategy (the so-called "hammer and dance" strategy), while Sweden's strategy was based on advising on social distancing, while keeping society open (a so-called mitigative strategy). Read More

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Nuclear overexpression levels of MAGE-A3 predict poor prognosis in patients with prostate cancer.

APMIS 2021 Jun 13;129(6):291-303. Epub 2021 Apr 13.

Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.

Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. In this study, for the first time, we performed immunohistochemical analysis to evaluate the expression of MAGE-A3 in 153 prostate tissue samples including prostate cancer (PCa), benign prostatic hyperplasia (BPH), and high-grade prostatic intraepithelial neoplasia (HPIN). Increased both nuclear and cytoplasmic expression of MAGE-A3 was significantly found in PCa tissues compared with both HPIN and BPH tissues (nuclear expression at p = 0. Read More

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A simple, safe and sensitive method for SARS-CoV-2 inactivation and RNA extraction for RT-qPCR.

APMIS 2021 Mar 17. Epub 2021 Mar 17.

Division of Clinical Neurology, Nuffield Department of Clinical Neurosciences, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.

The SARS-CoV-2 pandemic has created an urgent need for diagnostic tests to detect viral RNA. Commercial RNA extraction kits are often expensive, in limited supply, and do not always fully inactivate the virus. Together, this calls for the development of safer methods for SARS-CoV-2 extraction that utilize readily available reagents and equipment present in most standard laboratories. Read More

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Regulation of the expression of human endogenous retroviruses: elements in fetal development and a possible role in the development of cancer and neurological diseases.

APMIS 2021 May 5;129(5):241-253. Epub 2021 Apr 5.

Department of Biomedicine, Aarhus University, Aarhus C, Denmark.

Human endogenous retroviruses (HERVs) are remnants of ancient retroviral germline infections. Most HERV sequences are silenced in somatic cells, but interest is emerging on the involvement of HERV derived transcripts and proteins in human physiology and disease. A HERV-W encoded protein, syncytin-1, has been co-opted into fetal physiology, where it plays a role in trophoblast formation. Read More

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Epidemics before microbiology: Stories from the plague in 1711 and cholera in 1853 in Copenhagen.

Authors:
Adam Bencard

APMIS 2021 Mar 7. Epub 2021 Mar 7.

Medical Museion and NNF Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

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Historical and contemporary views on cholera transmission: are we repeating past discussions? Can lessons learned from cholera be applied to COVID-19?

APMIS 2021 Mar 1. Epub 2021 Mar 1.

Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.

Cholera, a devastating diarrheal disease that caused several global pandemics in the last centuries, may share some similarities with the new COVID-19. Cholera has affected many populations in history and still remains a significant burden in developing countries. The main transmission route was thought to be predominantly through contaminated drinking water. Read More

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Autopsies in pandemics - a perspective on barriers and benefits. Is it time for a revival?

APMIS 2021 Mar 1. Epub 2021 Mar 1.

Department of Forensic Medicine, Section of Forensic Pathology, University of Copenhagen, Copenhagen, Denmark.

Influenza virus and coronavirus pandemics regularly sweep the globe, at great cost of health and economy. Our aim was to conduct a PubMed search for autopsy studies on influenza and coronavirus to investigate the contribution of autopsies during pandemics, focussing on autopsy methods and procedures and the role of autopsy findings in pandemics. The retrieved autopsy studies generally relied on microscopy, polymerase chain reaction (PCR), immunostaining and electron microscopy. Read More

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A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study.

APMIS 2021 Mar 1. Epub 2021 Mar 1.

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.

In this descriptive pilot study, we aim to establish a porcine Staphylococcus aureus skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 S. Read More

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Animal models of invasive mycoses.

APMIS 2021 Mar 1. Epub 2021 Mar 1.

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.

Animal models of invasive fungal infections have been developed and are applied in a huge number of different animal species for a number of research purposes, for example, the study of pathogenesis, defense mechanisms, and therapeutic strategies. From the different models, which in most cases are based on the same fungal species and often the same strain, as in spontaneous human infections, fundamental results and knowledge of the diagnosis, progression, prophylaxis, and therapy have been achieved. However, in all models, one should be critical with respect to mimicking the disease entity of humans, which is often the focus of the research. Read More

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On the history of human coronaviruses.

APMIS 2021 Feb 24. Epub 2021 Feb 24.

Lund University, Lund, Sweden.

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February 2021