4,216 results match your criteria Apmis[Journal]


Introduction.

APMIS 2019 Apr 15. Epub 2019 Apr 15.

Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.

Personalized medicine is the tailoring of medical treatment to a specific subset of patients who are usually identified by genetic markers or other molecular profiling strategies. In addition, personalized medicine-or precision medicine-can be based on biomarkers identified using non-molecular techniques or additional factors related to a specific patient, still individualizing the treatment. The field of personalized medicine has developed extremely rapidly during the last 10 to 15 years alongside the impressive technical developments within the field of molecular biology and awareness of the need to include additional parameters in treatment planning. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12952DOI Listing

Antibiotic therapy as personalized medicine - general considerations and complicating factors.

APMIS 2019 Apr 14. Epub 2019 Apr 14.

Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.

The discovery of antibiotic drugs is considered one of the previous century's most important medical discoveries (1). Appropriate use of antibiotics saves millions of lives each year and prevents infectious complications for numerous people. Still, infections kill unacceptable many people around the world, even in developed countries with easy access to most antibiotic drugs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12951DOI Listing
April 2019
1 Read

Cytopathological Features of Secretory Carcinoma of Salivary Glands and Ancillary Techniques in Its Diagnostics: Impact of New Milan System for Reporting Salivary Gland Cytopathology.

APMIS 2019 Apr 3. Epub 2019 Apr 3.

Pathology, Fimlab Laboratories, Arvo Ylpön katu 4, 33520, Tampere, Finland.

Cytopathological Features of Secretory Carcinoma of Salivary Glands and Ancillary Techniques in Its Diagnostics: Impact of a New Milan System for Reporting Salivary Gland Cytopathology Secretory carcinoma (SC) of salivary glands is a newly described low-grade malignancy characterized by the presence of ETV6 rearrangement. Only a few cases and very small series with cytomorphology were reported so far. Six cases of fine-needle aspirations (FNA) from afterward histologically, immunohistochemically and genetically confirmed SCs were retrieved from the archives of the authors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12950DOI Listing
April 2019
3 Reads

Measurable residual disease testing for personalized treatment of acute myeloid leukemia.

APMIS 2019 Mar 28. Epub 2019 Mar 28.

Department of Pathology, Halland Hospital Halmstad, Region Halland, Halmstad, Sweden.

This review summarizes - with the practicing hematologist in mind - the methods used to determine measurable residual disease (MRD) in everyday practice with some future perspectives, and the current knowledge about the prognostic impact of MRD on outcome in acute myeloid leukemia (AML), excluding acute promyelocytic leukemia. Possible implications for choice of MRD method, timing of MRD monitoring, and guidance of therapy are discussed in general and in some detail for certain types of leukemia with specific molecular markers to monitor, including core binding factor (CBF)-leukemias and NPM1-mutated leukemias. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12926DOI Listing

The synthetic antimicrobial peptide LTX21 induces inflammatory responses in a human whole blood model and a murine peritoneum model.

APMIS 2019 Mar 27. Epub 2019 Mar 27.

Paediatric Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway.

The global spread of antimicrobial resistance and the increasing number of immune-compromised patients are major challenges in modern medicine. Targeting bacterial virulence or the human host immune system to increase host defence are important strategies in the search for novel antimicrobial drugs. We investigated the inflammatory response of the synthetic short antimicrobial peptide LTX21 in two model systems: a human whole blood ex vivo model and in a murine in vivo peritoneum model - both reflecting early innate immune response. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12946DOI Listing

Characterization of CRISPR-Cas systems in Leptospira reveals potential application of CRISPR in genotyping of Leptospira interrogans.

APMIS 2019 Apr;127(4):202-216

Department of Medical Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou, China.

Leptospirosis is a zoonotic disease caused by pathogenic Leptospira. However, understanding of the pathogenic mechanism of Leptospira is still elusive due to the limited number of genetic tools available for this microorganism. Currently, the reason for the genetic inaccessibility of Leptospira is still unknown. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/apm.12935
Publisher Site
http://dx.doi.org/10.1111/apm.12935DOI Listing
April 2019
7 Reads
1.922 Impact Factor

Outbreak of Methicillin-resistant Staphylococcus aureus in a Hospital Center for Children's and Women's Health in a Swedish County.

APMIS 2019 Apr;127(4):181-186

Department of Infection Control and Hygiene, Linköping University Hospital, Linköping, Sweden.

The objective of this study was to investigate a sudden increase in methicillin-resistant Staphylococcus aureus (MRSA) cases primarily in one maternity ward at the Center for Children's and Women's Health at Linköping University Hospital, Sweden. Approximately 300 individuals including patients, their family members, and healthcare workers were screened for MRSA. The antibiotic susceptibility was tested and isolates polymerase chain reaction (PCR)-positive for the mecA gene were spa typed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12929DOI Listing
April 2019
4 Reads

Managing costs in primary immunodeficiency: minimal immunophenotyping and three national references.

APMIS 2019 Apr;127(4):228-235

Pediatric Allergy and Immunology/Center of Investigation in Pediatrics (CIPED), Faculty of Medical Sciences, State University of Campinas - Unicamp, Sao Paulo, Brazil.

Our aim was to evaluate the cost-effectiveness of a minimal lymphocyte subset quantification (LSQ) by flow cytometry as the first screening in children with clinically suspected primary immunodeficiency (PID). Two hundred sixty-eight Brazilian patients (0-21 years old) were studied. They were divided by clinical and phenotypical features into those fulfilling criteria for PID (PID phenotype) according to the 2017 International Union of Immunological Societies (IUIS) classification and those not fulfilling these criteria (non-PID phenotype). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12932DOI Listing

Antimicrobial susceptibility of Neisseria gonorrhoeae isolates and treatment of gonorrhoea patients in Ternopil and Dnipropetrovsk regions of Ukraine, 2013-2018.

APMIS 2019 Mar 23. Epub 2019 Mar 23.

WHO Collaborating Centre for Gonorrhoea and other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a major public health concern globally. However, recent gonococcal AMR data from Eastern Europe are extremely limited and no AMR data for strains spreading in Ukraine has ever been internationally published. We investigated the AMR of N. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12948DOI Listing
March 2019
2 Reads

Phyllodes tumor with a benign heterologous osseous component: a diagnostic challenge.

APMIS 2019 Mar 22. Epub 2019 Mar 22.

Department of Pathology, Copenhagen University Hospital, Rigshospitalet, København Ø, Denmark.

Phyllodes tumors (PTs) are rare fibroepithelial neoplasms of the breast and constitute 0.3-1% of all primary breast tumors. They should be characterized in to a benign, borderline or malignant category based on a combination of histological features. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12949DOI Listing
March 2019
2 Reads

Evaluation of two tuberculosis PCR assays for routine use in a clinical setting of low population and low tuberculosis prevalence.

APMIS 2019 Mar 22. Epub 2019 Mar 22.

Department of Clinical Microbiology, Fimlab Laboratories Ltd., Tampere, Finland.

Today, there are numerous different molecular diagnostic assays for the detection of tuberculosis (TB), allowing the optimization of rapid detection of TB according to the clinical need. In this study, two high-throughput TB PCR assays with combined antimicrobial resistance detection, Anyplex™ II MTB/MDR (Seegene) and RealTime MTB + RealTime MTB RIF/INH Resistance (Abbott Molecular), were evaluated for routine use in a clinical setting of low population and low TB prevalence in Finland. The RealTime MTB assay was 100% concordant (22/22 positive, n = 169) with the reference methods (culture and Xpert MTB/RIF PCR assay, Cepheid). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12947DOI Listing
March 2019
4 Reads

Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease.

APMIS 2019 Apr 12;127(4):187-195. Epub 2019 Mar 12.

Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences & Research Centre, Belgavi, Karnataka, India.

Periodontal disease is an oral inflammatory disease that destroys the tooth supporting periodontal tissues resulting in tooth loss. Porphyromonas gingivalis is a keystone pathogen that plays a significant role in periodontitis. In previous studies, resveratrol has shown significant results by targeting inflammatory and adhesive markers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12930DOI Listing
April 2019
2 Reads

Epigenetic therapy in hematological cancers.

APMIS 2019 Mar 12. Epub 2019 Mar 12.

Department of Hematology, Rigshospitalet, University Hospital Copenhagen, Copenhagen, Denmark.

The increasing depth of knowledge about cancer biology throughout the last decades, has underlined the importance of not only genetic aberrations, but also epigenetic abnormalities in cancer cells. The extensive exploration of the cancer epigenome has provided insights into key pathways involved in tumorigenesis, as well as has allowed the development of novel epigenetic therapies. In this review, we will present the important role of epigenetic alterations in hematological diseases, with special focus on epigenetically-based targeting of hematological malignancies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12906DOI Listing

Direct detection and characterization of Clostridium difficile from a novel collection device to improve laboratory workflow.

APMIS 2019 Mar 4. Epub 2019 Mar 4.

Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.

Emergence of Clostridium difficile strains with increased virulence emphasizes the importance of early diagnosis and surveillance of C. difficile infection (CDI). In this study, the new FecalSwab™ collection and transport system was evaluated to improve C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12942DOI Listing

Role of HPV DNA, HPV mRNA and cytology in the follow-up of women treated for cervical dysplasia.

APMIS 2019 Apr 27;127(4):196-201. Epub 2019 Feb 27.

Department of Molecular and Translational Medicine, Institute of Microbiology, University of Brescia-Spedali Civili, Brescia, Italy.

The aim of this study was to assess the role of cytology, human papilloma virus (HPV) DNA and human papilloma virus messenger RNA (HPV mRNA) assays in detecting cervical intraepithelial neoplasia grade 2+ (CNi 2+) (recurrences/persistence) during the follow-up of women after treatment of cervical intraepithelial lesion. This cross-sectional study was performed among 43 women treated for cervical intraepithelial neoplasia (CIN) between January 2014 and January 2017 at the Department of Obstetrics and Gynecology of Spedali Civili's Hospital, Brescia, Italy. Pap smear and cervical samples for HPV tests were collected during the follow-up visit. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12931DOI Listing
April 2019
1 Read

An update on head and neck cancer: new entities and their histopathology, molecular background, treatment, and outcome.

APMIS 2019 Feb 27. Epub 2019 Feb 27.

Department of Otorhinolaryngology Head and Neck Surgery and Audiology and, Rigshospitalet, Copenhagen, Denmark.

The head and neck region harbor numerous specialized tissues of all lineages giving rise to a plethora of different malignancies. In recent years, new types and subtypes of cancer has been described here due to the recognition of their histological and molecular characteristics. Some have been formally accepted in the most recent classifications from the World Health Organization (WHO) and American Joint Committee on Cancer (AJCC) as distinct diseases due to characteristics in clinical presentation, outcome, and treatment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12901DOI Listing
February 2019
1 Read
1.922 Impact Factor

Downregulation of the cytochrome P450 4B1 protein confers a poor prognostic factor in patients with urothelial carcinomas of upper urinary tracts and urinary bladder.

APMIS 2019 Apr;127(4):170-180

Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.

The objective of this study was to examine the expression level of cytochrome P450 4B1 (CYP4B1) protein and its clinical significance in specimens from patients with urothelial carcinomas (UC) including upper tract urothelial carcinoma (UTUC, n = 340) and urinary bladder urothelial carcinoma (UBUC, n = 295). Data mining on public domains identified five potential candidate transcripts which were downregulated in advanced UBUCs, indicating that it might implicate in UC progression. Immunohistochemistry was performed to analyze the CYP4B1 protein levels on 635 tissues from UC patients retrospectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12939DOI Listing

Rapid identification of Candida sp. by MALDI-TOF mass spectrometry subsequent to short-term incubation on a solid medium.

APMIS 2019 Apr;127(4):217-221

Parasitology Mycology Department, Jean Minjoz University Hospital, Besancon, France.

Rapid identification of Candida species is important for appropriate antifungal therapy of fungemia. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system is a useful tool to identify bacteria and yeasts. In this study, we evaluated the feasibility of identifying yeasts after a short-term incubation on a solid medium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12936DOI Listing
April 2019
1 Read

Molecular typing of Clostridioides difficile isolates from clinical and non-clinical samples in Iran.

APMIS 2019 Apr;127(4):222-227

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Clostridioides difficile is a major cause of nosocomial infectious diarrhea in hospitalized patients throughout the world. We aimed to characterize C. difficile isolates among hospitalized patients, hospital staffs, and hospital environment samples obtained in three tertiary care hospitals of Iran with regard to their molecular types between June 2016 and November 2017. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/apm.12937
Publisher Site
http://dx.doi.org/10.1111/apm.12937DOI Listing
April 2019
3 Reads

PTEN immunohistochemistry in endometrial hyperplasia: which are the optimal criteria for the diagnosis of precancer?

APMIS 2019 Apr;127(4):161-169

Gynecology and Obstetrics Unit, Department of Neuroscience, Reproductive Sciences and Dentistry, School of Medicine, University of Naples Federico II, Naples, Italy.

Guidelines recommend protein phosphatase and tensin homolog (PTEN) immunohistochemistry for differentiating between benign endometrial hyperplasia (BEH) and atypical endometrial hyperplasia/endometrioid intraepithelial neoplasia (AEH/EIN). However, it is unclear when PTEN expression should be defined as 'lost' and thus suggestive of AEH/EIN. We aimed to determine the optimal immunohistochemical criteria to define PTEN loss in endometrial hyperplasia, through a systematic review and meta-analysis of diagnostic accuracy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12938DOI Listing
April 2019
1.922 Impact Factor

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells.

APMIS 2019 Feb 25. Epub 2019 Feb 25.

Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.

It is known that earthworm coelomic fluid (CF) can affect not only cancer but also normal cells. The study demonstrated that the CF of the earthworm Dendrobaena veneta exhibited cytotoxicity against A549 lung cancer cells but did not toward the bronchial epithelial cell line BEAS-2B. The selective effect on the tumor cells was achieved after a short-term CF heat pre-treatment at 70 °C. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12941
Publisher Site
http://dx.doi.org/10.1111/apm.12941DOI Listing
February 2019
2 Reads

Targeting anaplastic lymphoma kinase in neuroblastoma.

APMIS 2019 Feb 25. Epub 2019 Feb 25.

Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Over the last decade, anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase (RTK), has been identified as a fusion partner in a diverse variety of translocation events resulting in oncogenic signaling in many different cancer types. In tumors where the full-length ALK RTK itself is mutated, such as neuroblastoma, the picture regarding the role of ALK as an oncogenic driver is less clear. Neuroblastoma is a complex and heterogeneous tumor that arises from the neural crest derived peripheral nervous system. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12940DOI Listing
February 2019

Antimicrobial resistance, prevalence of resistance genes, and molecular characterization in intestinal Bacteroides fragilis group isolates.

APMIS 2019 Feb 25. Epub 2019 Feb 25.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Since the level of antimicrobial resistance in Bacteroides fragilis has increased, monitoring the antimicrobial susceptibility could be necessary. The objectives of this study were to (i) investigate the prevalence of species, the occurrence of reduced antimicrobial susceptibility (E-test method), and antibiotic resistance genes in the B. fragilis group and (ii) evaluate the prevalence of enterotoxigenic B. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12943DOI Listing
February 2019
3 Reads

Complexity of glandular architecture should be reconsidered in the classification and management of endometrial hyperplasia.

APMIS 2019 Feb 25. Epub 2019 Feb 25.

Gynecology and Obstetrics Unit, Department of Neuroscience, Reproductive Sciences and Dentistry, School of Medicine, University of Naples Federico II, Naples, Italy.

The 2014 World Health Organization (WHO) classification of endometrial hyperplasia (EH) defines premalignant EH based on only cytologic atypia, disregarding architecture complexity. We aimed to assess the impact of architecture complexity on the risk of cancer in non-atypical EH. A systematic review and meta-analysis was performed by searching electronic databases form their inception to October 2018. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12945DOI Listing
February 2019
1.922 Impact Factor

IL-2/IL-6 ratio correlates with liver function and recovery in acute liver injury patients.

APMIS 2019 Feb 25. Epub 2019 Feb 25.

Translational Medicine Research Center, Affiliated Jiangning Hospital, and Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, China.

Acute liver injury can result from a number of different diseases. Inflammatory cytokines are known to be involved in the development of this condition; however, their precise roles and effects on liver function remain unclear. The goal of this study was to determine the relationship between serum cytokine levels and both the severity of liver damage and recovery in acute liver injury. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12944
Publisher Site
http://dx.doi.org/10.1111/apm.12944DOI Listing
February 2019
23 Reads
1.922 Impact Factor

Toward liquid biopsies in cancer treatment: application of circulating tumor DNA.

APMIS 2019 Feb 19. Epub 2019 Feb 19.

Center for Genomic Medicine, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.

Circulating tumor DNA (ctDNA) refers to the fraction of cell-free DNA in a patient's blood originating from tumor cells. Increased knowledge about tumor genomics, improvements in targeted therapies, and accompanying advances in DNA-sequencing technologies have increased the interest in using ctDNA as a minimally invasive tool in cancer diagnostics and treatment. Especially, early tumor detection including identification of minimal residual disease and stratification of adjuvant therapy are promising approaches. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12912DOI Listing
February 2019

Cystic fibrosis - an example of personalized and precision medicine.

APMIS 2019 Feb 14. Epub 2019 Feb 14.

Cystic Fibrosis Center, Rigshospitalet, Copenhagen, Denmark.

Cystic fibrosis (CF) is a severe, monogenic, autosomal recessive disease caused by mutations in the CFTR (cystic fibrosis transmembrane regulator) gene, where disturbed chloride and bicarbonate transportation in epithelial cells results in a multiorgan disease with primarily pulmonary infections and pancreatic insufficiency. In 1968, the Copenhagen CF Center was established, and centralized care of CF patients with monthly control was introduced. Close monitoring and treatment of Pseudomonas lung infection as well as segregation of patients with different infection status improved the clinical outcome as well as survival. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12915DOI Listing
February 2019
1 Read

Immunogenicity of HPV and HBV vaccines: adjuvanticity of synthetic analogs of monophosphoryl lipid A combined with aluminum hydroxide.

APMIS 2019 Mar;127(3):150-157

Department of Cell Therapy and Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Monophosphoryl lipid A (MPL), a purified and detoxified product of lipopolysaccharide (LPS) of Salmonella minnesota R595, has been used as an adjuvant in different vaccines. In this study, the efficacy of human papillomaviruses (HPV) and hepatitis B virus (HBV) vaccines formulated with aluminum hydroxide combined with two different synthetic MPLs, 3D-(6-acyl)-PHAD or 3D-PHAD, or aluminum hydroxide combined with the mixtures of such MPLs, has been assessed. The immunogenicity in female BALB/c mice was verified by two intramuscular injections of differently formulated HPV and HBV vaccines and the total immunoglobulin G (IgG) antibody response was considered to compare the employed adjuvants. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12927
Publisher Site
http://dx.doi.org/10.1111/apm.12927DOI Listing
March 2019
11 Reads

Chlamydia pneumoniae enhances Interleukin 8 (IL-8) production with reduced azithromycin sensitivity under hypoxia.

APMIS 2019 Mar;127(3):131-138

Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Obligate intracellular bacterium Chlamydia pneumoniae causes respiratory tract infections such as community-acquired pneumonia. During infection, C. pneumoniae induces inflammatory responses in host cells and the oxygen concentration at the infection sites subsequently decreases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12924DOI Listing
March 2019
3 Reads

Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori-infected patients.

APMIS 2019 Mar;127(3):139-149

Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.

Helicobacter pylori (Hp) may initiate autoimmunity as a result of molecular mimicry. The aim of this study was to compare the level of IgG antibodies to a specific epitope (P1 peptide) of human heat shock protein (Hsp)60 homologous to Hp Hsp60 (HspB) in the sera of healthy donors (HD), patients with Hp-related gastritis or coronary heart disease (CHD), uninfected or with Hp infection confirmed by rapid urease test, histological examination (dyspeptic patients) the C urea breath test ( C UBT), and anti-Hp antibodies (healthy donors, CHD patients). The Anti-P1 IgG induction by Hp was verified by adsorption of sera with these bacteria and by experimental immunization of Caviae porcellus with Hp. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12925
Publisher Site
http://dx.doi.org/10.1111/apm.12925DOI Listing
March 2019
4 Reads

Biomarkers in tumors of the central nervous system - a review.

APMIS 2019 Feb 10. Epub 2019 Feb 10.

Department of Genetics and Pathology, Laboratory Medicine, Lund, Sweden.

Until recently, diagnostics of brain tumors were almost solely based on morphology and immunohistochemical stainings for relatively unspecific lineage markers. Although certain molecular markers have been known for longer than a decade (combined loss of chromosome 1p and 19q in oligodendrogliomas), molecular biomarkers were not included in the WHO scheme until 2016. Now, the classification of diffuse gliomas rests on an integration of morphology and molecular results. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12916DOI Listing
February 2019

The role of Tenascin-C and Twist1 in gastric cancer: cancer progression and prognosis.

APMIS 2019 Feb;127(2):64-71

Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, China.

The aim of the present study was to identify the relation between Tenascin-C (TNC) and Twist1 and determine their clinical significance in gastric cancer (GC). We analyzed the expression of TNC and Twist1 in 159 GC samples and in 91 non-tumor samples using immunohistochemistry. In this study, TNC expression in stromal fibroblasts of GC was remarkably higher than non-tumor gastric lesions. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12919
Publisher Site
http://dx.doi.org/10.1111/apm.12919DOI Listing
February 2019
6 Reads

Carbamoyl phosphate synthetase 1 (CPS1) as a prognostic marker in chronic hepatitis C infection.

APMIS 2019 Feb;127(2):93-105

Hepatogastroenterology Department, Theodor Bilharz Research Institute, Giza, Egypt.

This study aims to assess the value of carbamoyl phosphate synthetase 1 (CPS1), as a non-invasive serum marker, for the evolution of chronic HCV infection and hepatic fibrosis. Seventy-two patients with HCV positive serum RNA and 15 health volunteers were enrolled in this study. Out of 72 patients, 10 patients had decompensated liver with ascites. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12917
Publisher Site
http://dx.doi.org/10.1111/apm.12917DOI Listing
February 2019
14 Reads

Establishment and effects of allograft and synthetic bone graft substitute treatment of a critical size metaphyseal bone defect model in the sheep femur.

APMIS 2019 Feb;127(2):53-63

BONESUPPORT AB, Lund, Sweden.

Assessment of bone graft material efficacy is difficult in humans, since invasive methods like staged CT scans or biopsies are ethically unjustifiable. Therefore, we developed a novel large animal model for the verification of a potential transformation of synthetic bone graft substitutes into vital bone. The model combines multiple imaging methods with corresponding histology in standardized critical sized cancellous bone defect. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12918
Publisher Site
http://dx.doi.org/10.1111/apm.12918DOI Listing
February 2019
6 Reads

Homologous role of CovRS two-component regulatory system in NAD -glycohydrolase activity in Streptococcus dysgalactiae subsp. equisimilis as in Streptococcus pyogenes.

APMIS 2019 Feb;127(2):87-92

Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Streptococcal toxic shock syndrome (STSS) is primarily caused by Streptococcus pyogenes, but it may also be caused by Streptococcus dysgalactiae subsp. equisimilis (SDSE). The analyses of S. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12914DOI Listing
February 2019
4 Reads

GB virus type C cross-reactivity in clinical samples with a low hepatitis C virus antibody positive response.

APMIS 2019 02;127(2):109-111

Lab of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" IRCCS, Rome.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12921DOI Listing
February 2019
1 Read

Whole genome sequencing of breast cancer.

APMIS 2019 Jan 28. Epub 2019 Jan 28.

Centre for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Breast cancer was the first to take advantage of targeted therapy using endocrine therapy, and for up to 20% of all breast cancer patients a further significant improvement has been obtained by HER2-targeted therapy. Greater insight in precision medicine is to some extent driven by technical and computational progress, with the first wave of a true technical advancement being the application of transcriptomic analysis. Molecular subtyping further improved our understanding of breast cancer biology and has through a new tumor classification enabled allocation of personalized treatment regimens. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12920
Publisher Site
http://dx.doi.org/10.1111/apm.12920DOI Listing
January 2019
11 Reads

Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance.

APMIS 2019 Mar 27;127(3):115-122. Epub 2019 Jan 27.

Faculty of Medicine, Microbiology Section, Bam University of Medical Sciences, Bam, Iran.

In this study, clinical ampicillin-resistant Enterococcus faecium isolates with minimum inhibitory concentrations (MICs) for ampicillin in the ranges from 128 to ˃512 μg/mL (n = 17) and two ampicillin-susceptible isolates (MIC 1 μg/mL) were investigated. No β-lactamase production was detected in these isolates. Alterations in the C-terminal part of pbp5 and levels of pbp5 mRNA expression were investigated by sequencing and quantitative real-time qRT-PCR, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12922DOI Listing

Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method.

APMIS 2019 Mar 27;127(3):123-130. Epub 2019 Jan 27.

Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.

While the detrimental effects of periprosthetic joint infections (PJIs) are well known, the process of biofilm formation on orthopaedic hardware is unclear. Previous work has shown that encasement of explant hardware in agar can aid in identifying biofilms. This study tested the utility of agar 'candle dip' method in detecting and mapping the location of biofilm on infected orthopedic components. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12923
Publisher Site
http://dx.doi.org/10.1111/apm.12923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372321PMC
March 2019
10 Reads
1.922 Impact Factor

The inflamed sputum in lower respiratory tract infection: l-lactate levels are correlated to neutrophil accumulation.

APMIS 2019 Feb 6;127(2):72-79. Epub 2019 Jan 6.

Department of Pulmonary and Infectious Diseases, Nordsjaellands Hospital, Hillerød, Denmark.

Lower respiratory tract infections (LRTI) are common, but little is known about the response of biomarkers of inflammation in the lungs. Therefore, our primary aim was to compare the concentration of l-lactate to the concentration of neutrophils in sputum and systemic markers of infection. Because it is difficult to differentiate viral and bacterial infection on the basis of clinical presentation in LRTI, our secondary aim was to evaluate if l- and d-lactate may serve as markers of local inflammation as representatives of neutrophils and bacteria, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12913DOI Listing
February 2019
1 Read

Mycobacterium brisbanense lung infection facilitated by steroid induced adrenal insufficiency.

APMIS 2019 Feb 25;127(2):106-108. Epub 2018 Dec 25.

Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.

Mycobacterium brisbanense is rapid-growing nontuberculous mycobacteria. It was first described in 2004 as a human pathogen and only one case report has previously been published. We report a case of M. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12911DOI Listing
February 2019
1 Read

Accurate prediction of anti-phagocytic activity of Vibrio vulnificus by measurement of bacterial adherence to hydrocarbonsPrediction of Anti-Phagocytic Activity.

APMIS 2019 Feb 21;127(2):80-86. Epub 2018 Dec 21.

Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.

Vibrio vulnificus can cause necrotizing soft tissue infection via exposure through an open wound, and the incubation period in cases of wound infection is only about 16 h. These facts strongly suggest that mechanisms to evade innate immune cell phagocytosis are essential for its pathogenicity. Hydrophobic interaction is one of the binding mechanisms between bacteria and phagocytes. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12910
Publisher Site
http://dx.doi.org/10.1111/apm.12910DOI Listing
February 2019
10 Reads

Primary signet-ring stromal tumour of the testis: case report with literature review.

APMIS 2019 Jan;127(1):45-49

Institute of Pathology, University of Göttingen, Göttingen, Germany.

Primary signet-ring stromal tumour of the testis (PSRSTT) is a very rare type of primary testicular tumour, which is not actually mentioned in the current WHO categorization of tumours of the male genital organs. In this case we report about histologically and immunohistochemically features of this rare tumour. In the present case, PSRTT was found in the orchiectomy preparation of a 69-year-old man with painless swelling of his left testicle. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12907DOI Listing
January 2019
4 Reads

The diabetic phenotype is preserved in myotubes established from type 2 diabetic subjects: a critical appraisal.

Authors:
Michael Gaster

APMIS 2019 Jan;127(1):3-26

Laboratory for Molecular Physiology, Department of Pathology and Department of Endocrinology, Odense University Hospital, Odense, Denmark.

Cultured human myotubes offer a unique model to distinguish between primary and environmental factors in the aetiology of insulin resistance in human skeletal muscle. The objective of this review was to summarize our and other group studies on insulin resistance in human myotubes established from lean, obese and type 2 diabetes (T2D) subjects. Overall, studies of human myotubes established from lean, obese and T2D subjects clearly show that part of the diabetic phenotype observed in vivo is preserved in diabetic myotubes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12908DOI Listing
January 2019
2 Reads

Subtyping of intraductal papillary mucinous neoplasms - pitfalls of MUC1 immunohistochemistry.

APMIS 2019 Jan 22;127(1):27-32. Epub 2018 Nov 22.

Department of Pathology, Copenhagen University Hospital Rigshospitalet, København, Denmark.

Intraductal papillary mucinous neoplasms (IPMNs) are precursor lesions of pancreatic ductal adenocarcinoma (PDAC). Current edition of WHO Classification of Tumors of the Digestive System recognizes four different subtypes (gastric, intestinal, pancreatobiliary, and oncocytic) and recommends analysis of mucin expression (MUC1, MUC2, MUC5AC, MUC6) as well as evaluation of architectural and cell differentiation patterns for correct classification. However, there is no consensus on MUC1 expression of IPMN-lesions in the literature. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12900DOI Listing
January 2019
4 Reads

Comparison of methods for measuring antibiotic consumption in an intensive care unit.

APMIS 2019 Jan;127(1):33-40

Department of Neuroanaesthesiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

Hospitals worldwide are working on minimizing unnecessary use of antimicrobials. To assess actual changes of antimicrobial usage, correct and precise measurements are necessary. This study aimed to compare data on the purchase of antibiotics from the pharmacy and the administration of antibiotics to patients, respectively, in an intensive care unit (ICU). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12909DOI Listing
January 2019
1 Read

Human native endocarditis caused by Streptococcus canis-a case report.

APMIS 2019 Jan;127(1):41-44

Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.

We report a very rare case of Streptococcus canis native infective endocarditis in a 73-year-old woman living in close contact with her dog. Her echocardiography showed large calcifications in the mitral annulus, massive regurgitation below the posterior leaflet, and adjacent vegetation. Blood culture was positive for Streptococcus Lancefield group G. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12905DOI Listing
January 2019
3 Reads

WHO laboratory validation of Xpert CT/NG and Xpert TV on the GeneXpert system verifies high performances.

APMIS 2018 Dec;126(12):907-912

WHO Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Effective tests for diagnosis of sexually transmitted infections (STIs), used point of care to inform treatment and management decisions, are urgently needed. We evaluated the analytical sensitivity and specificity of the Xpert CT/NG and Xpert TV tests, examining 339 samples spiked with phenotypically and/or genetically diverse strains of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis, and other related species that may cross-react. The APTIMA Combo 2 test and APTIMA TV test were used as reference tests. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12902
Publisher Site
http://dx.doi.org/10.1111/apm.12902DOI Listing
December 2018
8 Reads

The importance of early diagnosis of Mycobacterium abscessus complex in patients with cystic fibrosis.

APMIS 2018 Dec;126(12):885-891

Cystic Fibrosis Center Copenhagen, Rigshospitalet, Copenhagen, Denmark.

Mycobacterium abscessus complex can cause severe lung infections and has proven to be a serious threat to patients with cystic fibrosis and a challenge for clinicians due to difficulties in timely diagnosis and complex multidrug treatment regimes. Mycobacterial culture is the gold standard for diagnosis, but in most cystic fibrosis centers it is performed less frequently than culture for other pathogens. Consensus today recommends just one annual mycobacterial culture for asymptomatic patients with cystic fibrosis, a strategy likely to lead to diagnostic delays. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/apm.12903DOI Listing
December 2018
11 Reads

Autotaxin and vascular endothelial growth factor receptor-2 and -3 are related to vascular development during the progression of chronic viral hepatitis C.

APMIS 2018 Dec;126(12):913-921

Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan.

Vascular endothelial growth factor (VEGF) and autotaxin (ATX) play important roles in embryonic vasculogenesis and cancer progression. This study examines whether these two angiogenic factors cooperate in the mechanism that regulates vascular development during the progression of chronic viral hepatitis C (CVH-C) (Inuyama classification, F1-F4). First, surgical wedge biopsy specimens and needle biopsy specimens were obtained. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/apm.12904
Publisher Site
http://dx.doi.org/10.1111/apm.12904DOI Listing
December 2018
10 Reads