809 results match your criteria Annals of laboratory medicine[Journal]


Correspondence: Response to "Evaluating the Cumulative Impact of Ionizing Radiation Exposure With Diagnostic Genetics".

Ann Lab Med 2019 Jul;39(4):419-420

Department of Laboratory Medicine and Genetics, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.4.419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400718PMC
July 2019
2 Reads

Letter: Evaluating the Cumulative Impact of Ionizing Radiation Exposure With Diagnostic Genetics.

Authors:
Hai Qiang Wang

Ann Lab Med 2019 Jul;39(4):417-418

Institute of Integrative Medicine, Shaanxi University of Chinese Medicine, Xi'an, China.

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http://dx.doi.org/10.3343/alm.2019.39.4.417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400715PMC

Multicenter Comparison of Four Automated Immunoassay Analyzers for Prostate Specific Antigen.

Ann Lab Med 2019 Jul;39(4):406-410

Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.4.406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400726PMC

A Rare Case of Acute Myeloid Leukemia With Fusion and Massive Hyperdiploidy.

Ann Lab Med 2019 Jul;39(4):403-405

Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.4.403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400712PMC
July 2019
1 Read

Comparative Performance of the Reverse Algorithm Using Architect Syphilis TP Versus the Traditional Algorithm Using Rapid Plasma Reagin in Florida's Public Health Testing Population.

Ann Lab Med 2019 Jul;39(4):396-399

Bureau of Public Health Laboratories, Florida Department of Health, Jacksonville, FL, USA.

In Florida, where syphilis is a reportable disease, the number of primary and secondary (P&S) syphilis cases has increased from 3,266 in 2008-2010 to 5,340 in 2013-2015, a 63% increase. The objective of this study was to compare the performance and sensitivity of the syphilis reverse algorithm with the traditional algorithm for detecting P&S (infectious) syphilis cases. Clinical specimens from individuals who self-referred for syphilis testing at public health clinics were processed using the traditional algorithm (non-treponemal rapid plasma reagin (RPR) test followed by a confirmatory treponemal (EIA) test) and then further tested with the Architect Syphilis TP (ASTP) immunoassay (Abbott Diagnostics, Chicago, IL, USA) or by RPR confirmation, if needed (reverse algorithm). Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400719PMC
July 2019
2 Reads

Enumeration of CD34-positive Stem Cells Using the ADAMII Image-based Fluorescence Cell Counter.

Ann Lab Med 2019 Jul;39(4):388-395

Laboratory Development and Evaluation Center, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: It is very important to accurately enumerate CD34-positive (CD34+) cells for successful hematopoietic stem cell transplantation (HSCT). We evaluated the ability of the newly developed image based-immunofluorescence cell counter ADAMII (NanoEntek, Seoul, Korea) to enumerate CD34+ cells, which was improved through simultaneous CD45 analysis.

Methods: We enumerated CD34+ cells with ADAMII using 19 peripheral blood (PB) and 91 leukapheresis samples from HSCT donors. Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400716PMC
July 2019
2 Reads

Comparison of Six Automated Immunoassays With Isotope-Diluted Liquid Chromatography-Tandem Mass Spectrometry for Total Thyroxine Measurement.

Ann Lab Med 2019 Jul;39(4):381-387

Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: Accurate serum total thyroxine (TT4) measurement is important for thyroid disorder diagnosis and management. We compared the performance of six automated immunoassays with that of isotope-diluted liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) as the reference method. We also evaluated the correlation of thyroid stimulating hormone (TSH) with TT4 measured by ID-LC-MS/MS and immunoassays. Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400722PMC
July 2019
1 Read

Development and Evaluation of an Immunoglobulin Y-Based ELISA for Measuring Prostate Specific Antigen in Human Serum.

Ann Lab Med 2019 Jul;39(4):373-380

Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wrocław University of Science and Technology, Wrocław, Poland.

Background: Measurement of serum prostate specific antigen (PSA) concentrations remains one of the leading methods for diagnosing prostate cancer. We developed and evaluated an immunoglobulin Y (IgY)-based ELISA to measure total PSA (tPSA) concentrations in human serum that could be used as an alternative to commercially available diagnostic assays that rely on mouse monoclonal IgG.

Methods: A sandwich ELISA based on an anti-PSA IgY antibody was developed. Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400723PMC

Molecular Epidemiology of Human Astrovirus in Stool Samples From Patients With Acute Gastroenteritis in Korea, 2013-2017.

Ann Lab Med 2019 Jul;39(4):367-372

Department of Laboratory Medicine, Hallym Univerisity College of Medicine, Chuncheon, Korea.

Background: Human astroviruses (HAstVs) are one of the major causes of acute gastroenteritis. Classic HAstVs can be classified into eight genotypes. We investigated the positive rate of HAstVs and the distribution of HAstV genotypes in strains isolated from patients with acute gastroenteritis in Hwaseong, Korea, in 2013-2017. Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400717PMC
July 2019
1 Read

The Incidence and Immunophenotypic and Genetic Features of JL1 Expressing Cells and the Therapeutic Potential of an Anti-JL1 Antibody in Pediatric Acute Leukemias.

Ann Lab Med 2019 Jul;39(4):358-366

Department of Pediatrics, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.

Background: JL1 is a newly identified CD43 epitope that specifically recognizes leukemic cells. We analyzed the incidence of JL1 expression and compared the clinical, immunophenotypic, and genetic characteristics of pediatric acute leukemia patients with respect to JL1 expression status to determine the therapeutic potential of an anti-JL1 antibody.

Methods: Seventy-eight patients with pediatric acute leukemia (52 with ALL, 26 with AML) diagnosed between December 2014 and January 2016 were enrolled prospectively. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.4.358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400720PMC
July 2019
3 Reads

The Platelet-to-Lymphocyte Ratio as an Inflammatory Marker in Rheumatic Diseases.

Ann Lab Med 2019 Jul;39(4):345-357

Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, West Midlands, UK.

The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combined blood cell counts and their ratios. Several large observational studies have demonstrated the value of shifts in PLR in evaluating the severity of systemic inflammation and predicting infections and other comorbidities, in inflammatory rheumatic diseases. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.4.345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400713PMC
July 2019
5 Reads

Expanded Impacts of Platelet Functions: Beyond Hemostasis and Thrombosis.

Ann Lab Med 2019 Jul;39(4):343-344

Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.4.343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400714PMC

Septicemia Caused by Secondary to Pneumonia.

Ann Lab Med 2019 05;39(3):340-342

Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.3.340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340845PMC
May 2019
1 Read

Association Between Prolonged Closure Time on the Platelet Function Analyzer-200 and Risk of Perioperative Blood Transfusion.

Ann Lab Med 2019 May;39(3):330-332

Department of Laboratory Medicine, Hallym University College of Medicine, Hallym University Sacred Heart Hospital, Anyang, Korea.

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340840PMC
May 2019
9 Reads
1.481 Impact Factor

Age-Specific Cutoffs of the Sysmex UF-1000i Automated Urine Analyzer for Rapid Screening of Urinary Tract Infections in Outpatients.

Ann Lab Med 2019 May;39(3):322-326

Department of Laboratory Medicine, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Korea.

We investigated the usefulness of age-specific cutoffs for screening of urinary tract infections (UTIs) in Korean outpatients, using the automated urine analyzer UF-1000i (Sysmex, Kobe, Japan). We retrospectively reviewed outpatient medical records. Urine samples of 7,443 outpatients from January 2010 to December 2017 were analyzed using urine culture and UF-1000i. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340846PMC
May 2019
23 Reads

Laboratory Diagnosis of Infection in Korea: The First National Survey.

Ann Lab Med 2019 May;39(3):317-321

Department of Laboratory Medicine, Sanggye Paik Hospital, School of Medicine, Inje University, Seoul, Korea.

In May 2015, we conducted a voluntary online survey on laboratory diagnostic assays for infection (CDI) across clinical microbiology laboratories in Korea. Responses were obtained from 66 laboratories, including 61 hospitals and five commercial laboratories. Among them, nine laboratories reported having not conducted CDI assays. Read More

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http://dx.doi.org/10.3343/alm.2019.39.3.317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340851PMC

Reclassification of Acute Myeloid Leukemia According to the 2016 WHO Classification.

Ann Lab Med 2019 May;39(3):311-316

Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

We reviewed our leukemia database to reclassify 610 patients previously diagnosed as having acute myeloid leukemia (AML) according to the updated 2016 WHO classification. Nine patients were categorized as having myelodysplastic syndrome and myeloid neoplasms with germline predisposition. AML with recurrent genetic abnormalities accounted for 57. Read More

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http://dx.doi.org/10.3343/alm.2019.39.3.311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340847PMC
May 2019
5 Reads
1.481 Impact Factor

Chromosomal Microarray Analysis as a First-Tier Clinical Diagnostic Test in Patients With Developmental Delay/Intellectual Disability, Autism Spectrum Disorders, and Multiple Congenital Anomalies: A Prospective Multicenter Study in Korea.

Ann Lab Med 2019 May;39(3):299-310

Department of Rehabilitation Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: To validate the clinical application of chromosomal microarray analysis (CMA) as a first-tier clinical diagnostic test and to determine the impact of CMA results on patient clinical management, we conducted a multicenter prospective study in Korean patients diagnosed as having developmental delay/intellectual disability (DD/ID), autism spectrum disorders (ASD), and multiple congenital anomalies (MCA).

Methods: We performed both CMA and G-banding cytogenetics as the first-tier tests in 617 patients. To determine whether the CMA results directly influenced treatment recommendations, the referring clinicians were asked to complete a 39-item questionnaire for each patient separately after receiving the CMA results. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340852PMC
May 2019
12 Reads
1.481 Impact Factor

Detection of Autoantibodies Against Nucleoporin p62 in Sera of Patients With Primary Biliary Cholangitis.

Ann Lab Med 2019 May;39(3):291-298

Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland.

Background: Primary biliary cholangitis (PBC) is an autoimmune liver disease characterized by specific autoantibodies. We evaluated the prevalence of autoantibodies against nucleoporin p62 (anti-p62) in PBC patients' sera to determine whether it can be a marker for PBC, in comparison with other immunological and biochemical parameters. We validated the performance of our in-house ELISA technique. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340841PMC
May 2019
11 Reads

Neopterin Levels and Indoleamine 2,3-Dioxygenase Activity as Biomarkers of Immune System Activation and Childhood Allergic Diseases.

Ann Lab Med 2019 May;39(3):284-290

Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey.

Background: Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated neopterin, tryptophan, and kynurenine levels as biomarkersof the Th1 immune system activation and changes in IDO-1 activityin children with asthma, allergic rhinitis, and atopic dermatitis, as well as the relationship between these biomarkers and the total IgE level, age, and disease severity.

Methods: We divided 205 children (80 girls and 125 boys, four months to 17 years old) into four groups: controls, patients with asthma, patients with allergic rhinitis, and patients with atopic dermatitis. Read More

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http://dx.doi.org/10.3343/alm.2019.39.3.284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340854PMC
May 2019
3 Reads

Small Red Blood Cell Fraction on the UF-1000i Urine Analyzer as a Screening Tool to Detect Dysmorphic Red Blood Cells for Diagnosing Glomerulonephritis.

Ann Lab Med 2019 05;39(3):271-277

Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: Dysmorphic red blood cells (dRBCs) are first-line biomarkers for detecting glomerulonephritis (GN) in patients with hematuria. The UF-1000i system (Sysmex, Kobe, Japan), based on flow cytometry, provides small red blood cell (RBC) values (UF-1000i [UF]-%sRBCs). We evaluated the clinical application of UF-%sRBCs for detecting %dRBCs and GN. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340839PMC
May 2019
2 Reads

Dried Blood Spot Multiplexed Steroid Profiling Using Liquid Chromatography Tandem Mass Spectrometry in Korean Neonates.

Ann Lab Med 2019 05;39(3):263-270

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Seoul, Korea.

Background: Screening for congenital adrenal hyperplasia (CAH) using immunoassays for 17α-hydroxyprogesterone generates many false-positive results. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous quantification of nine steroid hormones in dried blood spot (DBS) samples, and established reference intervals for these hormones.

Methods: We examined our method for linearity, precision, accuracy, extraction recovery, and matrix effects and determined the reference intervals of cortisol, 17α-hydroxyproges-terone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, corticosterone, 11-deoxycorticosterone, testosterone, and progesterone in 1,146 DBS samples (from 272 preterm and 874 full-term neonates). Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340850PMC
May 2019
8 Reads

Diagnostic Accuracy of the Risk of Ovarian Malignancy Algorithm in Clinical Practice at a Single Hospital in Korea.

Ann Lab Med 2019 05;39(3):252-262

Department of Obstetrics and Gynecology, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: The risk of ovarian malignancy algorithm (ROMA) is used for assessing ovarian cancer risk in women with a pelvic mass. Its diagnostic accuracy is variable. We investigated whether the clinically acceptable minimal sensitivity of >80. Read More

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http://dx.doi.org/10.3343/alm.2019.39.3.252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340842PMC
May 2019
4 Reads

Analytical and Clinical Performance of the Nanopia Krebs von den Lungen 6 Assay in Korean Patients With Interstitial Lung Diseases.

Ann Lab Med 2019 05;39(3):245-251

Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

Background: Krebs von den Lungen 6 (KL-6) is a sensitive marker for diagnosing, monitoring, and predicting the prognoses of interstitial lung diseases (ILDs). This study aimed to evaluate the performance of the Nanopia KL-6 assay (Sekisui Medical, Tokyo, Japan) and to test the relationship between KL-6 concentrations and clinical results.

Methods: In total, 230 patients diagnosed as having ILDs were enrolled. Read More

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http://dx.doi.org/10.3343/alm.2019.39.3.245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340849PMC
May 2019
1.481 Impact Factor

Accurate and Rapid Measurement of Glycated Hemoglobin Using HLC-723 G11 Variant Mode.

Ann Lab Med 2019 05;39(3):237-244

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Background: Type II diabetes mellitus causes many complications, and its prevalence continues to increase in Korea. Accurate measurement of glycated Hb (HbA1c) is important because of its usefulness in diagnosis, follow-up, and prediction of prognosis. We tested the analytical performance of the HLC-723 G11 Variant Mode (G11vr; Tosoh Bioscience, Inc. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340843PMC
May 2019
9 Reads

Comparison of Clinical Performance Between BacT/Alert Virtuo and BacT/Alert 3D Blood Culture Systems.

Ann Lab Med 2019 05;39(3):278-283

Division of Nursing, Gwangju Health University, Gwangju, Korea.

Background: BacT/Alert Virtuo (BioMérieux, Durham, NC, USA) is a recently developed blood culture system that includes functions of automatic registration, loading, and unloading of the blood culture bottles, as well as measurement of blood volume. We compared the performances between the BacT/Alert Virtuo and 3D (BioMérieux) blood culture systems.

Methods: A total of 952 patients (1,904 sets) visiting an university-affiliated hospital in Korea for blood cultures were enrolled. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.3.278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340844PMC
May 2019
12 Reads

Erratum: Novel 4-bp Intronic Deletion (c.1560+3_1560+6del) in LEMD3 in a Korean Patient With Osteopoikilosis.

Authors:

Ann Lab Med 2019 03;39(2):235-236

This corrects the article on p. 540 in vol. 37, PMID: 28840995. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240525PMC

Advantages of Array Comparative Genomic Hybridization Using Buccal Swab DNA for Detecting Pallister-Killian Syndrome.

Ann Lab Med 2019 Mar;39(2):232-234

Department of Medicine of Laboratory, Section of Cytogenetics, Azienda Ospedaliero Universitaria Pisana, Pisa, Italy.

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http://dx.doi.org/10.3343/alm.2019.39.2.232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240520PMC
March 2019
1 Read

The First Case of Congenital Prekallikrein Deficiency in Korea With a Novel Pathogenic Variant (c.1198G>T).

Ann Lab Med 2019 Mar;39(2):229-231

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.2.229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240513PMC

Centromere Protein-F-like Pattern in a Patient With Rheumatoid Arthritis.

Ann Lab Med 2019 Mar;39(2):227-228

Department of Laboratory Medicine, College of Medicine, Hanyang University, Seoul, Korea.

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http://dx.doi.org/10.3343/alm.2019.39.2.227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240516PMC
March 2019
1 Read

Red Blood Cell Alloimmunization in Korean Patients With Myelodysplastic Syndrome and Liver Cirrhosis.

Ann Lab Med 2019 Mar;39(2):218-222

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Red blood cell (RBC) alloimmunization varies across human populations and ethnic groups. We evaluated the characteristics of RBC alloimmunization and compared the risk of alloimmunization in Korean patients with myelodysplastic syndrome (MDS) and liver cirrhosis (LC), two representative diseases in which chronic transfusion is required. In total, 115 MDS patients and 202 LC patients transfused with RBCs between 2013 and 2015 were retrospectively included. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240531PMC
March 2019
14 Reads

Simultaneous Detection of Glutamate Dehydrogenase and Toxin A/B: Comparison of the C. DIFF QUIK CHEK COMPLETE and RIDASCREEN Assays.

Ann Lab Med 2019 Mar;39(2):214-217

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Various commercial assays have recently been developed for detecting glutamate dehydrogenase (GDH) and/or toxin A/B to diagnose infection (CDI). We compared the performance of two assays for the simultaneous detection of GDH and toxin A/B, using 150 stool samples: C. DIFF QUIK CHEK COMPLETE (QCC; TechLab, Blacksburg, VA, USA) and RIDASCREEN GDH (RC-GDH) and Toxin A/B (RC-Toxin A/B; R-Biopharm, Darmstadt, Germany). Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240517PMC

Hemoglobin A1c Levels Are Slightly but Significantly Lower in Normoglycemic Subjects With the Hemoglobin E Phenotype.

Ann Lab Med 2019 Mar;39(2):209-213

Department of Laboratory Medicine, National University Hospital, Singapore.

Hb mutations can alter the structure, behavior, stability, or quantity of the globin chain produced. Some Hb variants shorten the erythrocyte life span, resulting in physiologically lower hemoglobin A1c (HbA1c) levels. The hemoglobin E (HbE) phenotype involves a single-nucleotide polymorphism that reduces β-globin chain synthesis. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240528PMC
March 2019
5 Reads

Estimated Glomerular Filtration Rates Show Minor but Significant Differences Between the Single and Subgroup Creatinine-Based Chronic Kidney Disease Epidemiology Collaboration Equations.

Ann Lab Med 2019 Mar;39(2):205-208

Department of Laboratory Medicine, College of Medicine, Ewha Womans University, Seoul, Korea.

The creatinine-based Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation can be calculated according to race, sex, and creatinine concentration (subgroup equation) or in the form expressed by one equation (single equation). Minor differences in the constants used in the CKD-EPI equations (subgroup vs single equations) could result in a significant difference in the estimated glomerular filtration rate (eGFR). We evaluated the impact of this difference in 79,709 Korean patients. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240521PMC
March 2019
11 Reads

Cluster Containing More Than 20 CD3-Positive Cells in Bone Marrow Biopsy Is a Candidate Prognostic Indicator in Peripheral T-Cell Lymphoma, Not Otherwise Specified.

Ann Lab Med 2019 Mar;39(2):200-204

Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.

Assessment of bone marrow (BM) involvement in peripheral T-cell lymphoma, not otherwise specified (PTCL) is straightforward in cases of extensive involvement but difficult in cases of minimal to partial involvement. We evaluated the usefulness of CD3 as an immunohistochemical marker for assessing BM involvement in PTCL patients. BM biopsies of 92 PTCL patients were immunohistochemically stained for CD3, CD4, CD8, CD20, and CD56, and evaluated by two hematopathologists. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240518PMC

Antimicrobial Susceptibility Patterns of Anaerobic Bacterial Clinical Isolates From 2014 to 2016, Including Recently Named or Renamed Species.

Ann Lab Med 2019 Mar;39(2):190-199

Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

Background: Anaerobic bacterial resistance trends may vary across regions or institutions. Regional susceptibility patterns are pivotal in the empirical treatment of anaerobic infections. We determined the antimicrobial resistance patterns of clinically important anaerobic bacteria, including recently named or renamed anaerobes. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240532PMC
March 2019
2 Reads

An Excel Macro for Determining Allelic and Sequence Types of Bacterial Clones in Multilocus Sequence Typing.

Ann Lab Med 2019 Mar;39(2):183-189

Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.

Background: Multilocus sequence typing (MLST) was designed to overcome the low discriminatory power and poor reproducibility of previous molecular typing schemes, and it is useful for inter-laboratory, inter-regional, and inter-national comparison of pathogenic clones. MLST includes labor-intensive sequencing processes and meticulous allelic/sequence type (ST) determination processes, often prone to error. We developed a free automated MLST determination program (MLST typer) based on the Visual Basic for Applications macro, which runs on Microsoft Excel. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240524PMC
March 2019
8 Reads

Comparative Evaluation Between the RealStar PCR Kit and the AmpliSens ()-FRT PCR Kit for Detecting in Non-HIV Immunocompromised Patients.

Ann Lab Med 2019 Mar;39(2):176-182

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Background: Real-time PCR is more sensitive than microscopic examination for detecting . We compared the performance of two assays for detecting DNA: the RealStar PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens ()-FRT PCR kit (InterLabService Ltd. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240529PMC
March 2019
23 Reads

Identification and Characterization of NDM-1-producing Hypervirulent (Hypermucoviscous) in China.

Ann Lab Med 2019 Mar;39(2):167-175

Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Background: Carbapenem-resistant hypervirulent (hypermucoviscous) (CR-HMKP) poses a significant public health challenge. We investigated its epidemiology and molecular characteristics in a tertiary care hospital in eastern China.

Methods: CR-HMKP were identified among 106 non-duplicated carbapenem-resistant isolates (from June 2013 to September 2017) using the string test. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240523PMC
March 2019
4 Reads

Impact of Community-Onset Methicillin-Resistant on Bacteremia in a Central Korea Veterans Health Service Hospital.

Ann Lab Med 2019 Mar;39(2):158-166

Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.

Background: No study has examined the epidemiology of methicillin-resistant (MRSA) bacteremia in Korean veterans' hospitals. We investigated the microbiological and clinical epidemiology of bacteremia at the central Veterans Health Services (VHS) hospital in Korea.

Methods: Patients with bacteremia were consecutively enrolled from February to August 2015. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240515PMC
March 2019
14 Reads

Comparison of High Sensitivity and Conventional Flow Cytometry for Diagnosing Overt Paroxysmal Nocturnal Hemoglobinuria and Detecting Minor Paroxysmal Nocturnal Hemoglobinuria Clones.

Ann Lab Med 2019 Mar;39(2):150-157

Department of Laboratory Medicine, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea.

Background: High sensitivity flow cytometry (HS-FCM) was recently developed for diagnosing paroxysmal nocturnal hemoglobinuria (PNH). We compared its performance with conventional flow cytometry (C-FCM) for diagnosing overt PNH and detecting minor (0.1-1%) PNH clones in aplastic anemia (AA)/low-grade myelodysplastic syndrome (MDS) patients. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240522PMC
March 2019
11 Reads

Development of a Novel Flow Cytometry-Based System for White Blood Cell Differential Counts: 10-color LeukoDiff.

Ann Lab Med 2019 Mar;39(2):141-149

Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: Flow cytometry (FCM) is commonly used to identify many cell populations. We developed a white blood cell (WBC) differential counting system for detecting abnormal cells using FCM incorporating 10 colors and 11 antibodies in a single tube, called "10-color LeukoDiff," and evaluated its performance.

Methods: Ninety-one EDTA-anti-coagulated peripheral blood samples from 76 patients were analyzed using 10-color LeukoDiff. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240530PMC
March 2019
11 Reads

Increased Prevalence of Thalassemia in Young People in Korea: Impact of Increasing Immigration.

Ann Lab Med 2019 Mar;39(2):133-140

Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.

Background: Thalassemia is highly prevalent in Southeast Asia but is rare in Korea; however, Southeast Asian immigrant population is recently rising in Korea. We investigated the prevalence of thalassemia in Korea in the context of increasing immigration.

Methods: This prospective, observational, multicenter study was conducted between September 2015 and August 2017. Read More

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http://dx.doi.org/10.3343/alm.2019.39.2.133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240526PMC
March 2019
13 Reads

T-Cell Receptor Rearrangements Determined Using Fragment Analysis in Patients With T-Acute Lymphoblastic Leukemia.

Ann Lab Med 2019 Mar;39(2):125-132

Hematologic Oncology Clinic, Center for Specific Organs Cancer Research Institute & Hospital, National Cancer Center, Goyang, Korea.

Background: Chromosomal abnormalities and common genetic rearrangements related to T-acute lymphoblastic leukemia (T-ALL) are not clear. We investigated T-cell receptor () rearrangement in Korean T-ALL patients by fragment analysis, examining frequency, association between clinicopathologic characteristics and clonality, and feasibility for detecting minimal residual disease (MRD).

Methods: In 51 Korean patients diagnosed as having T-ALL, rearrangement was analyzed using the IdentiClone gene clonality assay (InVivoScribe Technologies, San Diego, CA, USA) from archived bone marrow specimens. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39
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http://dx.doi.org/10.3343/alm.2019.39.2.125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240512PMC
March 2019
34 Reads