Search our Database of Scientific Publications and Authors

I’m looking for a

    4585 results match your criteria Annals of clinical biochemistry[Journal]

    1 OF 92

    ANNALS EXPRESS: Development of a simple indocyanine green measurement method using an automated biochemical analyzer.
    Ann Clin Biochem 2017 Jan 1:4563217745895. Epub 2017 Jan 1.
    Chiba University.
    Background The indocyanine green (ICG) retention rate is important for assessing the severity of liver disorders. In the conventional method, blood needs to be collected twice. In the present study, we developed an automated ICG method that does not require blood sampling before intravenous ICG injections and is applicable to an automated biochemical analyzer. Read More

    ANNALS EXPRESS: Large abnormal peak on capillary zone electrophoresis due to contrast agent.
    Ann Clin Biochem 2017 Jan 1:4563217745896. Epub 2017 Jan 1.
    St. George's Hospital.
    Background: Some iodinated radio-contrast media absorb UV light and can therefore be detected by capillary zone electrophoresis (CZE). If seen, these peaks are typically small with "quantifications" of below 5g/L. Here we describe detection of a large peak on CZE that was due to the radio-contrast agent, Omnipaque(T)(M). Read More

    ANNALS EXPRESS: An ICP-MS Method For Relative Free Copper Determination And Generation of a Paediatric Reference Interval.
    Ann Clin Biochem 2017 Jan 1:4563217744809. Epub 2017 Jan 1.
    University Hospital Southampton NHS Foundation Trust.
    Background - Diagnosis of Wilson's disease is currently performed using caeruloplasmin as a first line screening test however this test has well described limitations. Monitoring of known Wilson's disease patients often uses 24 hour urine collection however this is inaccurate in children. Methods for directly measuring plasma free copper have been described but no reference interval data exist for a paediatric population. Read More

    ANNALS EXPRESS: Gestational age-specific reference intervals for 15 biochemical measurands during normal pregnancy in China.
    Ann Clin Biochem 2017 Jan 1:4563217738801. Epub 2017 Jan 1.
    Aims: Physiological changes that occur during pregnancy can influence biochemical parameters. Therefore, using reference intervals based on specimens from non-pregnant women to interpret laboratory results during pregnancy may be inappropriate. This study aimed to establish essential to establish reference intervals for a range of analytes during pregnancy. Read More

    Performance of autotaxin as a serum marker for liver fibrosis.
    Ann Clin Biochem 2017 Jan 1:4563217741509. Epub 2017 Jan 1.
    1 Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
    Background Because autotaxin reportedly has a better performance than hyaluronic acid as a marker for liver fibrosis for the prediction of cirrhosis caused by hepatitis C, we aimed to further evaluate the role of autotaxin in liver fibrosis of other aetiologies. Methods Autotaxin antigen was measured in serum samples from 108 patients with chronic hepatitis B and 128 patients with non-alcoholic fatty liver disease who had undergone a liver biopsy as well as healthy subjects and patients with chronic kidney disease, diabetes mellitus, rheumatoid arthritis and cardiac dysfunction. Results When evaluated using receiver operator characteristics curves, the performance of autotaxin for the prediction of significant fibrosis (F2-F4) in chronic hepatitis B patients was better than that of hyaluronic acid or type IV collagen 7S. Read More

    Dysregulation of serum microRNA-574-3p and its clinical significance in hepatocellular carcinoma.
    Ann Clin Biochem 2017 Jan 1:4563217741908. Epub 2017 Jan 1.
    1 Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong, JS, P. R. China.
    Objectives To explore microRNA-574-3p expression in serum of patients with hepatocellular carcinoma and investigate correlations between serum microRNA-574-3p expression and the development and prognosis of hepatocellular carcinoma. Design and methods Serum samples were collected from 70 patients with primary hepatocellular carcinoma, 40 patients with cirrhosis and 45 healthy controls. Serum microRNA-574-3p expression levels were detected by real-time quantitative polymerase chain reaction. Read More

    Effects of birth weight on profiles of dried blood amino-acids and acylcarnitines.
    Ann Clin Biochem 2017 Jan 1:4563216688038. Epub 2017 Jan 1.
    Department of Genetics and Metabolism, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
    Background Birth weight influences profiles of dried blood amino-acids and acylcarnitines in newborn screening. This study aimed to define a more appropriate cut-off value to reduce the false positive rate and the number of recalled patients in newborn screening. Methods All babies who underwent newborn screening in our center were included; they were divided into groups by birth weight: 2500-3999 g (comparator group), <1000 g (group 1), 1000-1499 g (group 2), 1500-2499 g (group 3), and >4000 g (group 4). Read More

    Biomarkers in mesothelioma.
    Ann Clin Biochem 2017 Jan 1:4563217741145. Epub 2017 Jan 1.
    Academic Respiratory Unit, School of Clinical Sciences, University of Bristol, Bristol, UK.
    Mesothelioma is an aggressive cancer of pleural and peritoneal cells that is difficult to diagnose and monitor. Numerous studies have attempted to identify a blood- or pleural fluid-based biomarker that could be used in the diagnostic pathway. More recently, there has been interest in the ability of serum/plasma biomarkers to monitor mesothelioma, given the development of newer treatments and limitations of radiological assessment. Read More

    Biochemistry of the menopause.
    Ann Clin Biochem 2017 Jan 1:4563217739930. Epub 2017 Jan 1.
    Institute of Women's Health, University College London, London, UK.
    The life of a human female is characterized from teenage years by monthly menstruation which ceases (the menopause) typically between the age of 40 and 60 years. The potential for reproduction declines and ceases as the ovaries become depleted of follicles. A transition period in mid-life, for 2 to 10 years, when menstruation is less regular is called the perimenopause. Read More

    Clinical evaluation and treatment of phaeochromocytoma.
    Ann Clin Biochem 2017 Jan 1:4563217739931. Epub 2017 Jan 1.
    4 Department of Endocrine Surgery, Royal Liverpool and Broadgreen University Hospitals Trust, Liverpool, UK.
    Phaeochromocytoma and extra adrenal paraganglioma are rare neuroendocrine tumours and have the potential to secrete adrenaline, noradrenaline and dopamine causing a myriad of clinical symptoms. Prompt diagnosis is essential for clinicians and requires a multidisciplinary specialist approach for the clinical and laboratory investigation, diagnosis, treatment and follow-up of patients. This paper is an integrated review of the clinical and laboratory evaluation and treatment of patients suspected to have phaeochromocytoma or paraganglioma, highlighting recent developments and best practices from recent published clinical guidelines. Read More

    Serum α-melanocyte-stimulating hormone may act as a protective biomarker for non-traumatic osteonecrosis of the femoral head.
    Ann Clin Biochem 2017 Jan 1:4563217738802. Epub 2017 Jan 1.
    4 Department of Orthopedics, Linyi People's Hospital, Linyi, Shandong Province, China.
    Background The α-melanocyte-stimulating hormone (α-MSH), an endogenous neuropeptide derived from proopiomelanocortin (POMC), has been identified to suppress inflammation and prevent osteoblast damage. Objective The present study was aimed to investigate the role of serum α-MSH in non-traumatic osteonecrosis of the femoral head (ONFH). Methods Seventy-nine patients diagnosed with non-traumatic ONFH and 79 sex- and age-matched healthy controls were enrolled in the study. Read More

    A combined liquid chromatography tandem mass spectrometry assay for the quantification of urinary oxalate and citrate in patients with nephrolithiasis.
    Ann Clin Biochem 2017 Jan 1:4563217739035. Epub 2017 Jan 1.
    Department of Clinical Biochemistry, 5295 Manchester University NHS Foundation Trust , Manchester, UK.
    Background Analysis of citrate and oxalate in a 24-h urine sample is important in the screening and monitoring of patients with nephrolithiasis. To streamline the analytical process, it was decided to combine oxalate and citrate and analyse them simultaneously in the same assay. Objective A highly sensitive and specific assay for analysis of urine citrate and oxalate was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a simple weak anion exchange solid phase extraction (WAX SPE) clean-up procedure. Read More

    Macrocomplexes and discordant high-sensitivity cardiac troponin concentrations.
    Ann Clin Biochem 2017 Jan 1:4563217734883. Epub 2017 Jan 1.
    4 Coronary Care Unit, Hamilton General Hospital, Hamilton Health Sciences, Hamilton, ON, Canada.
    Background Analytical comparisons between different high-sensitivity cardiac troponin (hs-cTn) assays are important for reassurance of results performed with different methodologies and to identify potential interferences or confounders to result interpretation. Our objective in the present study was to compare Beckman Coulter's latest hs-cTnI assay to Abbott's hs-cTnI assay and to assess agreement between results. Methods Two hundred ethylenediaminetetraacetic acid plasma samples that had clinically reported hs-cTnI results from the Abbott ARCHITECTi2000 that spanned the analytical range were stored (median = 4 h), re-centrifuged and retested for hs-cTnI on the Abbott ARCHITECTi1000 and Beckman Coulter Access2 analysers. Read More

    Association of subcutaneous and visceral fat with circulating microRNAs in a middle-aged Japanese population.
    Ann Clin Biochem 2017 Jan 1:4563217735124. Epub 2017 Jan 1.
    5 Department of Public Health, Fujita Health University School of Health Sciences, Toyoake, Japan.
    Purpose It has been demonstrated that circulating microRNA profiles are affected by physiological conditions. Several studies have demonstrated that microRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating microRNAs and obesity, which has become a major public health problem worldwide. Read More

    Evaluation of the revised Schwartz creatinine-based glomerular filtration rate estimating equation in Black African children in KwaZulu-Natal, South Africa.
    Ann Clin Biochem 2017 Jan 1:4563217734573. Epub 2017 Jan 1.
    1 Department of Chemical Pathology, Nelson R Mandela Medical School, 72753 University of KwaZulu-Natal , Durban, South Africa.
    Background The use of serum creatinine equations for estimating glomerular filtration rate is well known in adults and children. We evaluated the revised Schwartz creatinine-based estimated glomerular filtration rate prediction equation in Black African children in KwaZulu-Natal, South Africa. Methods Review of medical records of all Black African patients aged 2-18 years old who have had glomerular filtration rate determined by intravenous Technetium-99 m-diethylene-triamine-pentaacetate, for the period 1 January 2010 to 31 December 2014 at the Inkosi Albert Luthuli Central Hospital, Durban, South Africa was performed. Read More

    ANNALS EXPRESS: Serum high sensitive C reactive protein is associated with dietary intakes in diabetic patients with and without hypertension: a cross-sectional study.
    Ann Clin Biochem 2017 Jan 1:4563217733286. Epub 2017 Jan 1.
    Brighton and Sussex Medical School.
    Background: Serum high sensitive C-reactive protein (hs-CRP) concentrations independently predict the development of diabetes, metabolic syndrome and cardiovascular disease. However, the impact of dietary factors on serum hs-CRP concentrations in diabetic patients has received limited attention. We aimed to investigate the association between dietary factors and hs-CRP, among diabetic patients with and without hypertension and healthy subjects. Read More

    Development of a novel fluorescent activity assay for lecithin:cholesterol acyltransferase.
    Ann Clin Biochem 2017 Jan 1:4563217733285. Epub 2017 Jan 1.
    1 Lipoprotein Metabolism Section, Cardio-Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
    Background Lecithin:cholesterol acyltransferase (LCAT) is a plasma enzyme that esterifies cholesterol. Recombinant human LCAT (rhLCAT) is now being developed as an enzyme replacement therapy for familial LCAT deficiency and as a possible treatment for acute coronary syndrome. The current 'gold standard' assay for LCAT activity involves the use of radioisotopes, thus making it difficult for routine clinical use. Read More

    Increased serum concentrations of N(ɛ)-carboxymethyllysine are related to the presence and the severity of rheumatoid arthritis.
    Ann Clin Biochem 2017 Jan 1:4563217733500. Epub 2017 Jan 1.
    1 Laboratory of Biochemistry, LR12ES05, Faculty of Medicine, University of Monastir, Monastir, Tunisia.
    Background There are limited data regarding the contribution of advanced glycation end products in the presence of rheumatoid arthritis. We investigated whether serum N(ɛ)-carboxymethyllysine and pentosidine were related to the presence and the severity of rheumatoid arthritis. Methods Eighty patients with rheumatoid arthritis and 30 control subjects were included in a cross-sectional study. Read More

    Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays.
    Ann Clin Biochem 2017 Jan 1:4563217701777. Epub 2017 Jan 1.
    1 Melbourne Pathology, Collingwood, Australia.
    Background Biotin interference is a significant problem to which at-risk laboratories must now be attuned. We sought to systematically characterize the nature of this interference in Roche immunoassays. Methods Known concentrations of biotin were titrated into serum samples and the effects on competitive and sandwich immunoassays were analysed. Read More

    The development of an artificial stool usable for the surveillance of faecal haemoglobin testing.
    Ann Clin Biochem 2017 Jan 1:4563217732587. Epub 2017 Jan 1.
    3 1st Department of Integrated Medicine ,Saitama Medical Center, Jichi University, Saitama, Japan.
    Background Faecal occult blood testing is an important diagnostic tool for the detection of colorectal cancer. However, it has not been standardized due to the absence of suitable specimens for surveillance. Methods We developed a ready-to-use artificial stool made from rice flour. Read More

    Influence of cytochrome P450 genotype on the plasma disposition of prochlorperazine metabolites and their relationships with clinical responses in cancer patients.
    Ann Clin Biochem 2017 Jan 1:4563217731432. Epub 2017 Jan 1.
    1 Department of Hospital Pharmacy, 12793 Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
    Background Oral prochlorperazine, a dopamine D2 receptor antagonist, is largely metabolized to sulphoxide, 7-hydroxylate and N-desmethylate by cytochrome P450s (CYPs). This study evaluated the influence of CYP genotype on the plasma dispositions of prochlorperazine and its metabolites and their relationships with antiemetic efficacy and prolactin elevation in cancer patients. Methods Forty-eight cancer patients treated with oral prochlorperazine were enrolled. Read More

    Suboptimal rise in awakening-induced cortisol is an accurate marker of cortisol insufficiency in patients with normal renal function (eGFR >60 mL/min).
    Ann Clin Biochem 2017 Jan 1:4563217732361. Epub 2017 Jan 1.
    1 4616 King's College London , London, UK.
    Background The insulin tolerance test is the gold standard for diagnosis of cortisol insufficiency. However, it is cumbersome, invasive, requires supervised hospital facilities and has unpleasant side-effects. A non-invasive outpatient-based test will be useful. Read More

    ANNALS EXPRESS: Associations between glycaemic control and activation of the Renin Angiotensin Aldosterone System in participants with Type 2 Diabetes Mellitus and Hypertension.
    Ann Clin Biochem 2017 Jan 1:4563217728964. Epub 2017 Jan 1.
    Galway University Hospitals.
    Introduction: Hyperglycaemia increases succinate levels and succinate receptor (GPR91) activation in the kidney resulting in renin release. The aim of our study was to determine if there is an association between glycaemic control as evidenced by glycated haemoglobin (HbA1c) values and activation of the renin-angiotensin-aldosterone-system in patients with type 2 diabetes mellitus (T2DM) and hypertension.

    Methods: A cross-sectional study was conducted at Galway University Hospitals between December 2014 and March 2015. Read More

    Altered mean platelet volume in children with Henoch-Schonlein purpura and its association with disease activity.
    Ann Clin Biochem 2017 Jan 1:4563217727015. Epub 2017 Jan 1.
    Department of Clinical Laboratory, the First Affiliated Hospital of Guangxi Medical University, Guangxi, China.
    Background Henoch-Schonlein purpura is a systemic small-vessel vasculitis that occurs mainly in children. A review of the literature has suggested a correlation between mean platelet volume and several inflammatory disorders. However, to the best of our knowledge, any potential correlation between mean platelet volume and Henoch-Schonlein purpura has not been reported in the literature. Read More

    Prediction of haematocrit in dried blood spots from the measurement of haemoglobin using commercially available sodium lauryl sulphate.
    Ann Clin Biochem 2017 Jan 1:4563217726809. Epub 2017 Jan 1.
    Department of Clinical Biochemistry, University Hospital of South Manchester, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK.
    Background When preparing dried blood spots (DBSs), haematocrit (Hct) can affect the ability of the blood to spread through the filter paper, thus resulting in varying quantities of sample being measured when fixed subpunches of the DBSs are taken. It may be important to predict the sample Hct to correct volume differences. Methods Blood (10  µL) was applied to Perkin Elmer 226® paper. Read More

    Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.
    Ann Clin Biochem 2017 Jan 1:4563217726808. Epub 2017 Jan 1.
    3 School of Medicine, University of Zagreb, Zagreb, Croatia.
    Introduction Despite some new treatment possibilities, the improvement in survival rate for hepatocellular carcinoma (HCC) patients is still poor due to late diagnosis. The aim of this study was to investigate the diagnostic sensitivity and specificity of protein induced by vitamin K absence or antagonist-II (PIVKAII), Glypican-3 (GP3), Cystatin B (CSTB), squamous cell carcinoma antigen 1 (SCCA1) and hepatocyte growth factor (HGF) as potential tumour markers for HCC in patients with alcoholic liver cirrhosis (ALC) using imaging techniques (MSCT and MRI) as reference standards. Patients and methods Eighty-three participants were included: 20 healthy volunteers, 31 patients with ALC and 32 patients with HCC. Read More

    False-negative results in the immunoassay analysis of drugs of abuse: can adulterants be detected by sample check test?
    Ann Clin Biochem 2017 Jan 1:4563217725089. Epub 2017 Jan 1.
    2 Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, RWTH University Hospital Aachen, Aachen, Germany.
    Background The dilution or adulteration of urine is a serious problem in drugs of abuse testing. Tests to identify adulteration are currently available. This study investigated the ability of the CEDIA® sample check to detect adulteration. Read More

    20α- and 20β-dihydrocortisone may interfere in LC-MS/MS determination of cortisol in saliva and urine.
    Ann Clin Biochem 2017 Jan 1:4563217724178. Epub 2017 Jan 1.
    1 Clinical Chemistry, Institution for Medical Biosciences, Umeå University, Umeå, Sweden.
    Background LC-MS/MS methods offer high selectivity in cortisol determinations. However, endogenous steroid metabolites may still interfere and compromise the results, for example in the diagnosis of Cushing's syndrome. Erroneously elevated cortisol may, in particular, be misleading at the low concentrations found in salivary samples obtained at late night and after dexamethasone suppression. Read More

    Experience with newer central nervous system autoantibodies.
    Ann Clin Biochem 2017 Jan 1:4563217724818. Epub 2017 Jan 1.
    2 Queen Elizabeth Neurosciences Centre, 1732 University Hospital Birmingham NHS Foundation Trust , Birmingham, UK.
    In the last decade, a large number of neuronal cell-surface antibodies have been described which are responsible for a range of neuroimmunological central nervous system disorders. Unlike the paraneoplastic antibodies which target intracellular antigens, these antibodies appear to be pathogenic and hence identification and prompt treatment can make a substantial impact on clinical outcomes of these patients. We review the common antibodies against the ionotropic glutamate receptors (NMDAR, AMPAR), metabotropic glutamate receptors (mGluR1 and mGluR5), voltage-gated potassium channel-complex proteins (LGI1, CASPR2), and other antibodies targeted against glycine receptor, glutamic acid decarboxylase, gamma-amino butyric acid B, dopamine-2-receptor and dipeptidyl-peptidase-like protein 6. Read More

    Application of NICE guideline NG12 to the initial assessment of patients with lower gastrointestinal symptoms: not FIT for purpose?
    Ann Clin Biochem 2017 Jan 1:4563217707981. Epub 2017 Jan 1.
    1 Centre for Research into Cancer Prevention and Screening, University of Dundee, Dundee, UK.
    Background The National Institute for Health and Care Excellence (NICE) published NG12 in 2015. The referral criteria for suspected colorectal cancer (CRC) caused controversy, because tests for occult blood in faeces were recommended. Faecal immunochemical tests for haemoglobin (FIT), which estimate faecal haemoglobin concentrations (f-Hb), might more than fulfil the intentions. Read More

    Demand management by electronic gatekeeping of test requests does not influence requesting behaviour or save costs dramatically.
    Ann Clin Biochem 2017 Jan 1:4563217707980. Epub 2017 Jan 1.
    1 Department of Chemical Pathology, Faculty of Health Sciences, University of Pretoria and National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa.
    Background Healthcare budgets face constraints, and laboratories have developed strategies to adapt to the concomitant increase in workload. Some of the tests (7.4%) may be attributed to unnecessary repeat testing. Read More

    Diagnostic accuracy of the Coopscore(©) to predict liver fibrosis in human immunodeficiency virus/hepatitis B virus co-infection.
    Ann Clin Biochem 2017 Jan 1:4563217707979. Epub 2017 Jan 1.
    1 Pôle de Biologie Médicale et Pathologie, Hôpitaux Universitaire de l'Est Parisien, site Saint-Antoine, Paris, France.
    Background Non-invasive methods for assessing liver fibrosis are increasingly used as an alternative to liver biopsy. Recently, a score-based biochemical blood test (Coopscore(©)) was developed in a cohort of patients chronically infected with hepatitis C virus, showing higher diagnostic performances than Fibrometer®, Fibrotest®, Hepascore® and Fibroscan™. Here, we assess its performance in patients co-infected with the human immunodeficiency virus and hepatitis B virus. Read More

    Decreased serum glicentin concentration in patients with severe and morbid obesity.
    Ann Clin Biochem 2017 Jan 1:4563217700172. Epub 2017 Jan 1.
    1 Clinical Chemistry Laboratory, University Hospital of Nice, Nice, France.
    Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in individuals with adult obesity and to study its potential link with various metabolic parameters. Read More

    Urinary albumin: a risk marker under-requested in primary care in Spain.
    Ann Clin Biochem 2017 Jan 1:4563217716475. Epub 2017 Jan 1.
    4 Department of Radiology, University of Virginia, Charlottesville, VA, USA.
    Background To study the inter-practice regional variation in the request of urinary albumin by general practitioners and compare to guideline recommendations. Methods A cross-sectional study enrolled laboratories from different Spanish autonomous communities. Laboratories were invited to report the number of urinary albumin requested by general practitioners during 2012 and 2014. Read More

    Foetal haemoglobin concentration at postmenstrual age is unaffected by gestational age at birth.
    Ann Clin Biochem 2017 Jan 1:4563217721253. Epub 2017 Jan 1.
    2 Infection Control and Prevention, Kobe University Hospital, Kobe, Japan.
    Background Our aim was to determine whether the postnatal age or postmenstrual age is a more appropriate criterion for evaluating foetal haemoglobin concentrations. Methods Blood samples ( n = 1095) were obtained from 394 infants and were divided into two groups based on gestational age at birth: <37 weeks ( n = 491) and ≥37 weeks ( n = 604). (1) Foetal haemoglobin concentrations divided by one month at age after birth were compared between the groups. Read More

    ANNALS EXPRESS: High Sensitivity Troponin T and I reflect Left Atrial Function being assessed by Cardiac Magnetic Resonance Imaging (cMRI).
    Ann Clin Biochem 2017 Jan 1:4563217714004. Epub 2017 Jan 1.
    Clinic Nuremberg.
    Background: Left atrial function (LAF) plays an interactive role between pulmonary and systemic circulation. Cardiac biomarkers, such as amino-terminal pro-brain natriuretic peptide (NT-proBNP) and troponins, might reflect cardiac function. This study aims to evaluate the association between high sensitivity troponins (hsTn) and LAF in patients undergoing cMRI. Read More

    Quantitative measurement of a candidate serum biomarker peptide derived from α2-HS-glycoprotein, and a preliminary trial of multidimensional peptide analysis in females with pregnancy-induced hypertension.
    Ann Clin Biochem 2017 Jan 1:4563217717748. Epub 2017 Jan 1.
    1 Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan.
    Purpose We previously attempted to develop quantitative enzyme-linked immunosorbent assay (ELISA) systems for the PDA039/044/071 peptides, potential serum disease biomarkers (DBMs) of pregnancy-induced hypertension (PIH), primarily identified by a peptidomic approach (BLOTCHIP®-mass spectrometry (MS)). However, our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein341-367), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using liquid chromatography-multiple reaction monitoring (LC-MRM)/MS, to explore the potential utility of PDA071 as a DBM for PIH. Read More

    Newborn screening for sickle cell disorders using tandem mass spectrometry: three years' experience of using a protocol to detect only the disease states.
    Ann Clin Biochem 2017 Sep 17;54(5):601-611. Epub 2017 Jul 17.
    5 Screening Division, Public Health Wales, Cardiff, UK.
    Background Tandem mass spectrometry (MS/MS) has recently become an alternative method for the newborn screening of sickle cell disorders (SCD), as it is able to detect haemoglobin (Hb) peptides following digestion of bloodspots with trypsin. Using the SpOtOn Diagnostics Reagent Kit, we previously developed a screening protocol to detect only the disease states of SCD, using action values based on the ratio between the variant Hb peptide to wild-type peptide abundances for the HbS, C, D(Punjab), O(Arab), E and Lepore peptides. Methods Action values using the ratios between the wild type HbA (ßT1-3) peptides and the foetal Hb (γT2) peptide were developed to identify bloodspot samples from premature and transfused infants. Read More

    Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method.
    Ann Clin Biochem 2017 Jan 1:4563217716474. Epub 2017 Jan 1.
    1 Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.
    Background We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light. Read More

    Changes in the albumin glycation site, plasma pentosidine and esRAGE concentrations before and after intensive diabetic treatment in patients with abnormally high glycated albumin levels.
    Ann Clin Biochem 2017 Jan 1:4563216687787. Epub 2017 Jan 1.
    1 Division of Central Laboratory, Daisan Hospital, Jikei University School of Medicine, Tokyo, Japan.
    Background We have reported that the blood glucose normalization treatment reduced the albumin glycation sites and the intensity of albumin AGE fluorescence in patients with abnormally high glycated albumin levels. To clarify the relationship between glycaemic control status and levels of glycated proteins and related markers, we studied the change of the markers of the DM patients with and without fatty liver, liver cirrhosis and dialysis before and after the intensive diabetic treatment. Methods Eight diabetic patients with abnormally high glycated albumin levels (no complications: 2, fatty liver: 3, liver cirrhosis: 2, dialysis: 1) were recruited. Read More

    Mind the gap: Shortcomings of the osmotic gap and a possible solution.
    Ann Clin Biochem 2017 Jan 1:4563217694052. Epub 2017 Jan 1.
    1 Department of Chemical Pathology, The Royal Brisbane Women's and Children's Hospital, Pathology Queensland, Queensland, Australia.
    Background Measured (MO) and calculated osmotic concentrations (CO) and the osmotic gap (OG) are commonly used in the investigation of electrolyte and volume disturbances as well as in cases of suspected volatile ingestion. Methods We compared 38 published formulae for CO with MO on a large data-set ( n = 9466) and adjusted the CO with the result of a Passing-Bablok regression procedure. Validation of this adjustment was performed with a separate data-set ( n = 2082). Read More

    Age and sex differences in serum adiponectin and its association with lipoprotein fractions.
    Ann Clin Biochem 2017 Jan 1:4563217699233. Epub 2017 Jan 1.
    1 Internal Medicine of Metabolism and Nutrition, The Jikei University Graduate School of Medicine, Chiba, Japan.
    Objective The correlation of adiponectin with cholesterol concentration of fractionated lipoproteins has not been well investigated. Methods This study included 174 subjects (79 men and 95 women) without diabetes. The medical record data were investigated retrospectively. Read More

    1 OF 92