4,692 results match your criteria Annals of clinical biochemistry[Journal]


Setting clinical performance specifications to develop and evaluate biomarkers for clinical use.

Ann Clin Biochem 2019 Apr 16:4563219842265. Epub 2019 Apr 16.

14 Department of Clinical Chemistry & Endocrinology, Prince of Wales Hospital, New South Wales Health Pathology and School of Medical Sciences, University of New South Wales, Randwick, Australia.

Background: Biomarker discovery studies often claim 'promising' findings, motivating further studies and marketing as medical tests. Unfortunately, the patient benefits promised are often inadequately explained to guide further evaluation, and few biomarkers have translated to improved patient care. We present a practical guide for setting minimum clinical performance specifications to strengthen clinical performance study design and interpretation. Read More

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April 2019
6 Reads

The pathway for introducing novel examination procedures in routine practice in accordance with ISO 15189:2012: 17-Hydroxy progesterone, dehydroepiandrosterone sulphate and vitamin D as examples.

Ann Clin Biochem 2019 Apr 11:4563219835582. Epub 2019 Apr 11.

1 Department of Medicine - DIMED, University of Padova, Padova, Italy.

Background: In a medical laboratory, changes may be made to the analytical phase of diagnostic testing whenever a new test or the issue of a 'new generation' kit or new diagnostic system is required. In such cases, ISO 15189:2012 accreditation can assist laboratory professionals. The aim of the present study was to propose a working pathway for introducing new examination procedures into clinical practice in accordance with the ISO 15189:2012 standard, through the exemplars of 17-hydroxy progesterone, dehydroepiandrosterone sulphate and vitamin D. Read More

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http://dx.doi.org/10.1177/0004563219835582DOI Listing

High concentration of plasma methoxytyramine: dopamine-producing tumour or Parkinson's disease therapy?

Ann Clin Biochem 2019 Apr 11:4563219835263. Epub 2019 Apr 11.

1 Service of Clinical Pharmacology, University Hospital of Lausanne (CHUV), Lausanne, Switzerland.

Background: Levodopa (L-DOPA) provided to patients with Parkinson's disease causes an increase in dopamine and methoxytyramine blood concentration which may lead to erroneous diagnosis of dopamine-producing tumours based on a plasma fractionated metanephrines and methoxytyramine assay. Considering that oral L-DOPA is mainly transformed in the gut wall into dopamine and methoxytyramine, we hypothesize that patients treated with L-DOPA produce predominantly sulphated methoxytyramine, whereas dopamine-producing tumours, devoid of sulfotransferase, will secrete free methoxytyramine. These metabolic differences may allow for discrimination between the two groups of patients through methoxytyramine plasma concentration. Read More

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http://dx.doi.org/10.1177/0004563219835263DOI Listing
April 2019
1 Read

Comparison of the International Federation of Clinical Chemistry and Japan Diabetes Society reference methods for conversion to the National Glycohemoglobin Standardization Program values.

Ann Clin Biochem 2019 Apr 11:4563219834965. Epub 2019 Apr 11.

2 Clinical Research Center, Hidaka Hospital, Takasaki, Japan.

Background: The national programmes for the harmonization of haemoglobin A1c measurement in the US and Japan are based on differently designated comparison methods. The future basis for international standardization is expected to be the reference system developed by the International Federation of Clinical Chemistry (IFCC) Working Group on haemoglobin A1c Standardization. The aim of the present study is to compare the relationship between the IFCC reference method (RM) and Japanese Diabetes Society (JDS) RM used for the conversion to the National Glycohemoglobin Standardization Program (NGSP) values. Read More

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Changes of iron metabolism during pregnancy and the establishment of reference intervals for pregnant Chinese women.

Ann Clin Biochem 2019 Apr 11:4563219835853. Epub 2019 Apr 11.

1 Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Abnormalities of iron metabolism in pregnancy pose risks for maternal and fetal health. Robust reference intervals for iron metabolism indices have not been established in a pregnant Chinese population. The purpose of this study was to derive reference intervals for indices of iron metabolism during pregnancy in a Chinese population. Read More

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http://dx.doi.org/10.1177/0004563219835853DOI Listing

Test evaluation: major challengesahead - Opportunities abound.

Ann Clin Biochem 2019 Apr 11:4563219837301. Epub 2019 Apr 11.

3 Department of Clinical Epidemiology, Biostatistics & Bioinformatics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

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http://dx.doi.org/10.1177/0004563219837301DOI Listing

A novel assay for triglycerides using glycerol dehydrogenase and a water-soluble formazan dye, WST-8.

Ann Clin Biochem 2019 Apr 11:4563219830715. Epub 2019 Apr 11.

3 Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.

Background: The glycerol-3-phosphate (GPO)-peroxidase (POD) chromogenic method is one of the most widely used methods to assay triglycerides. However, it is well known that peroxidase is affected by reducing agents, and recently, it has been reported that some materials affect its activity. Moreover, there is a high possibility of non-specific reaction, as the method uses many enzymes. Read More

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http://dx.doi.org/10.1177/0004563219830715DOI Listing

Functional iron deficiency markers are absent during pregnancy despite evidence of low iron stores.

Ann Clin Biochem 2019 Apr 11:4563219837290. Epub 2019 Apr 11.

1 Department of Clinical Chemistry and Hematology, Gelre Hospital, Apeldoorn, Netherlands.

Introduction: Functional iron deficiency parameters predict iron-restricted erythropoiesis more precisely than ferritin. Ferritin and erythropoiesis can be affected by inflammation and hormonal alterations. We hypothesize that the association between low ferritin concentrations and iron-restricted erythropoiesis is not comparable between pregnant and non-pregnant women. Read More

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April 2019
7 Reads

The simultaneous quantification of candidate serum biomarker peptides for hypertensive disorders of pregnancy.

Ann Clin Biochem 2019 Apr 11:4563219839084. Epub 2019 Apr 11.

1 Institute for Environmental and Gender-specific Medicine, Graduate School of Medicine, Juntendo University, Chiba, Japan.

Background: Hypertensive disorders of pregnancy are defined as syndromes characterized by high blood pressure that develop after 20 weeks of pregnancy. The pathogenesis of hypertensive disorders of pregnancy has not been fully elucidated, and the most effective treatment is the termination of pregnancy. Therefore, methods for an early predictive diagnosis should be developed to rescue both the mother and the child. Read More

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http://dx.doi.org/10.1177/0004563219839084DOI Listing
April 2019
1 Read

Liquid biopsy for cancer diagnosis and screening - The promise and challenges.

Authors:
Mangesh A Thorat

Ann Clin Biochem 2019 Apr 11:4563219837592. Epub 2019 Apr 11.

1 Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary University of London, London, UK.

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http://dx.doi.org/10.1177/0004563219837592DOI Listing

Apolipoprotein profiling as a personalized approach to the diagnosis and treatment of dyslipidaemia.

Ann Clin Biochem 2019 Mar 19:4563219827620. Epub 2019 Mar 19.

1 Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands.

An elevated low-density lipoprotein cholesterol concentration is a classical risk factor for cardiovascular disease. This has led to pharmacotherapy in patients with atherosclerotic heart disease or high heart disease risk with statins to reduce serum low-density lipoprotein cholesterol. Even in patients in whom the target levels of low-density lipoprotein cholesterol are reached, there remains a significant residual cardiovascular risk; this is due, in part, to a focus on low-density lipoprotein cholesterol alone and neglect of other important aspects of lipoprotein metabolism. Read More

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http://dx.doi.org/10.1177/0004563219827620DOI Listing
March 2019
1 Read

Paediatric reference intervals for plasma anti-Müllerian hormone: comparison of data from the Roche Elecsys assay and the Beckman Coulter Access assay using the same cohort of samples.

Ann Clin Biochem 2019 Mar 19:4563219830733. Epub 2019 Mar 19.

1 Department of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester, UK.

Background: Autoanalyser methods for the measurement of anti-Müllerian hormone have been introduced into clinical laboratories but few reports of paediatric reference intervals using these new assays have been published.

Methods: After prior evaluation of the Roche Elecsys anti-Müllerian hormone assay against the Beckman Coulter modified second generation anti-Müllerian Hormone enzyme-linked immunosorbent assay using samples from adult females, a cohort of paediatric samples which had previously been assessed using the Beckman Coulter Access anti-Müllerian hormone assay was analysed using the Roche Elecsys anti-Müllerian hormone assay.

Results: The Roche Elecsys anti-Müllerian hormone assay measured significantly lower than the Beckman Coulter modified second generation anti-Müllerian Hormone enzyme-linked immunosorbent assay. Read More

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http://dx.doi.org/10.1177/0004563219830733DOI Listing

Validated liquid chromatography coupled to tandem mass spectrometry method for simultaneous quantitation of tolvaptan and its five major metabolites in human plasma.

Ann Clin Biochem 2019 Mar 4:4563219827045. Epub 2019 Mar 4.

1 Department of Hospital Pharmacy, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Background: Tolvaptan is converted to major metabolites including three monohydroxylates (DM-4110, DM-4111 and DM-4119), an oxidate (MOP-21826) and a carboxylate (DM-4103) in humans. This study developed a simultaneous quantitative method for tolvaptan and its five major metabolites in human plasma using liquid chromatography coupled to tandem mass spectrometry.

Methods: Deproteinized plasma specimens using acetonitrile were separated using a 3- μm particle size octadecylsilyl column with 250 mm length and a simple linear gradient program at a flow rate of 0. Read More

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http://dx.doi.org/10.1177/0004563219827045DOI Listing
March 2019
1 Read

High-throughput quantification of carboxymethyl lysine in serum and plasma using high-resolution accurate mass Orbitrap mass spectrometry.

Ann Clin Biochem 2019 Mar 4:4563219830432. Epub 2019 Mar 4.

1 Institute of Cardiovascular and Medical Sciences (ICAMS), BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK.

Background: Carboxymethyl lysine is an advanced glycation end product of interest as a potential biomarker of cardiovascular and other diseases. Available methods involve ELISA, with potential interference, or isotope dilution mass spectrometry (IDMS), with low-throughput sample preparation.

Methods: A high-throughput sample preparation method based on 96-well plates was developed. Read More

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http://dx.doi.org/10.1177/0004563219830432DOI Listing
March 2019
3 Reads

Symmetric dimethylarginine is a stronger predictor of mortality risk than asymmetric dimethylarginine among older people with kidney disease.

Ann Clin Biochem 2019 Feb 27:4563218822655. Epub 2019 Feb 27.

1 East Kent Hospitals University NHS Foundation Trust, Canterbury, Kent, UK.

Background: Circulating asymmetric dimethylarginine and symmetric dimethylarginine are increased in patients with kidney disease. Symmetric dimethylarginine is considered a good marker of glomerular filtration rate, while asymmetric dimethylarginine is a marker of cardiovascular risk. However, a link between symmetric dimethylarginine and all-cause mortality has been reported. Read More

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http://dx.doi.org/10.1177/0004563218822655DOI Listing
February 2019
2 Reads

Acute pseudogout - Measure serum magnesium.

Ann Clin Biochem 2019 Feb 27:4563219826169. Epub 2019 Feb 27.

4 Blood Sciences, The Royal Wolverhampton NHS Trust, Wolverhampton, UK.

We report a 49-year-old woman with an acute swollen left knee due to acute pseudogout with chondrocalcinosis as a presenting feature of Gitelman syndrome due a novel homozygous mutation of the SLC12A3 gene. This report highlights the under-recognized importance of excluding metabolic disease, including Gitelman syndrome, in younger patients whose sole presenting feature may be chondrocalcinosis with or without pseudogout, as this may impact on management and risk of further episodes. We also suggest that chondrocalcinosis and hypomagnesaemia with or without hypokalaemia are diagnostic of Gitelman syndrome. Read More

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http://dx.doi.org/10.1177/0004563219826169DOI Listing
February 2019
4 Reads

Evaluation of the efficacy of various reagents in improving microRNA extraction.

Ann Clin Biochem 2019 Feb 27:4563219828405. Epub 2019 Feb 27.

2 Department of Surgery, Daiyukai General Hospital, Ichinomiya, Aichi, Japan.

Background: MicroRNA has received considerable attention in the clinical context, and attempts are being made to use microRNA in clinical diagnosis. However, adequate quantities of microRNA required for analysis are challenging to isolate. We tested the effect of various reagents in improving microRNA extraction and compared their efficacy to that of a commercially available extraction kit (HighPure miRNA isolation kit, Roche). Read More

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http://dx.doi.org/10.1177/0004563219828405DOI Listing
February 2019
1 Read

A short report: Reflective testing in the diagnosis of hereditary haemochromatosis: Results of a short retrospective study.

Ann Clin Biochem 2019 Feb 27:4563219827614. Epub 2019 Feb 27.

York Teaching Hospitals NHS Foundation Trust, York, UK.

Background: Reflective addition of iron studies to elevated ferritin results can be a useful first step towards making a diagnosis of haemochromatosis; however, the criteria for doing so are poorly defined and the efficiency of different stages of this process are not well documented. We studied the efficiency of current practice at York Teaching Hospitals NHS Foundation Trust with the aim to identify areas for improvement.

Methods: Data were gathered from the laboratory database on the number of iron studies and subsequent interpretive comments reflectively added by laboratory staff during an eight-month period. Read More

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http://dx.doi.org/10.1177/0004563219827614DOI Listing
February 2019
2 Reads

Falsely elevated plasma 3-methoxytyramine in a patient receiving midodrine therapy.

Ann Clin Biochem 2019 Feb 27:4563218818199. Epub 2019 Feb 27.

Department of Clinical Biochemistry (Viapath), King's College Hospital NHS Foundation Trust, London, UK.

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http://dx.doi.org/10.1177/0004563218818199DOI Listing
February 2019

Biological variation of intact fibroblast growth factor 23 measured on a fully automated chemiluminescent platform.

Ann Clin Biochem 2019 Feb 27:4563219826161. Epub 2019 Feb 27.

1 Department of Laboratory Methods, Institute for Clinical and Experimental Medicine, Praha, Czech Republic.

Background: Fibroblast growth factor 23 (FGF23), a potent regulator of phosphate and vitamin D metabolism, is a new biomarker of kidney, bone and cardiovascular disorders. The aim of this study was to assess the biological variation of intact fibroblast growth factor 23 (iFGF23).

Methods: The within-subject (CV) and between-subject (CV) biological variations were assessed in 14 healthy volunteers in a six-week protocol (seven samples). Read More

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February 2019
4 Reads

Laboratory tests commonly used in complementary and alternative medicine: A review of the evidence.

Ann Clin Biochem 2019 Feb 27:4563218824622. Epub 2019 Feb 27.

3 Department of Clinical Biochemistry, Poole Hospital NHS Foundation Trust, UK.

It is increasingly easy for the general public to access a wide range of laboratory tests. Tests can be ordered online with little or no input from a health professional. The complementary and alternative medicine (CAM) community promote and sell a wide range of tests, many of which are of dubious clinical significance. Read More

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http://dx.doi.org/10.1177/0004563218824622DOI Listing
February 2019

A dynamic reference change value model applied to ongoing assessment of the steady state of a biomarker using more than two serial results.

Ann Clin Biochem 2019 Mar 25;56(2):283-294. Epub 2019 Feb 25.

3 Centre for Research into Cancer Prevention and Screening, University of Dundee, Dundee, Scotland.

Background: Reference change values are used to assess the significance of a difference in two consecutive results from an individual. Reference change value calculations provide the limits for significant differences between two results due to analytical and inherent biological variations. Often more than two serial results are available. Read More

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http://dx.doi.org/10.1177/0004563219826168DOI Listing

Clinical interpretation of prostate-specific antigen values: Type of applied cut-off value exceeds methods bias as the major source of variation.

Ann Clin Biochem 2019 Mar 24;56(2):259-265. Epub 2019 Feb 24.

1 Clinical Laboratory, Catharina Hospital, Eindhoven, the Netherlands.

Background: Prostate-specific antigen is the biochemical gold standard for the (early) detection and monitoring of prostate cancer. Interpretation of prostate-specific antigen is both dependent on the method and cut-off. The aim of this study was to examine the effect of method-specific differences and cut-off values in a national external quality assessment scheme (EQAS). Read More

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http://dx.doi.org/10.1177/0004563218822665DOI Listing

Comparing glomerular filtration rate equations and the impact of different creatinine assays on the assessment of renal function in cancer patients.

Ann Clin Biochem 2019 Mar 21;56(2):266-274. Epub 2019 Feb 21.

1 Department of Blood Sciences, Leeds General Infirmary, Old Medical School, Leeds, UK.

Background: Equations to estimate glomerular filtration rate based on serum creatinine are commonly used in cancer patients to assess renal function. However, there is uncertainty regarding which equation is most appropriate for this population and the impact of different creatinine assays.

Methods: Measured isotopic glomerular filtration rate results from 120 oncology patients were used to evaluate and compare all four versions of the Wright equation, Cockcroft and Gault, Modification of Diet in Renal Disease (MDRD), Chronic Kidney Disease Epidemiology Collaboration and the Janowitz and Williams formula; using eight different creatinine assays (five Jaffe, three enzymatic). Read More

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http://dx.doi.org/10.1177/0004563218822667DOI Listing

A survey of clinical laboratory instrument verification in the UK and New Zealand.

Ann Clin Biochem 2019 Mar 21;56(2):275-282. Epub 2019 Feb 21.

1 Sidra Medicine, Doha, Qatar.

Background: Clinical laboratory instrument verification testing is often an accreditation requirement. However, it is not known what verification procedures are in routine use or how often the process identifies problems which need addressing prior to testing clinical samples.

Objective: To investigate which standards are currently being used for laboratory verification in UK and New Zealand (NZ) clinical laboratories and to help establish if the activity justifies the effort required. Read More

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http://dx.doi.org/10.1177/0004563218823804DOI Listing
March 2019
2 Reads

The association of serum and vitreous adropin concentrations with diabetic retinopathy.

Ann Clin Biochem 2019 Mar 24;56(2):253-258. Epub 2019 Feb 24.

5 Department of Emergency, Qilu Hospital of Shandong University (Qingdao), Qingdao, China.

Objective: Adropin, a newly identified regulatory protein encoded by Enho gene, is correlated with insulin sensitivity and diabetes. The aim of this study is to determine whether serum and vitreous adropin concentrations are correlated with the presence of diabetic retinopathy.

Methods: A population of 165 patients with type 2 diabetes mellitus (52 without diabetic retinopathy, 69 with non-proliferative diabetic retinopathy and 44 patients with proliferative diabetic retinopathy) was enrolled in this study. Read More

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http://dx.doi.org/10.1177/0004563218820359DOI Listing
March 2019
7 Reads

Haematopoietic alterations in neonates with congenital diaphragmatic hernia receiving extracorporeal membrane oxygenation support.

Ann Clin Biochem 2019 Mar 24;56(2):247-252. Epub 2019 Feb 24.

2 Department of Neonatology and Pediatric Critical Care Medicine, University of Bonn Children's Hospital, Bonn, Germany.

Background: Extracorporeal membrane oxygenation procedure (ECMO) has been established in the therapy of respiratory insufficient infants with congenital diaphragmatic hernia. In congenital diaphragmatic hernia newborns, a delay in transfer to an ECMO centre is associated with a sharp increase in mortality. Predictive factors for ECMO support are urgently needed. Read More

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http://dx.doi.org/10.1177/0004563218820052DOI Listing
March 2019
8 Reads

Autotaxin and soluble IL-2 receptor concentrations in cerebrospinal fluids are useful for the diagnosis of central nervous system invasion caused by haematological malignancies.

Ann Clin Biochem 2019 Mar 24;56(2):240-246. Epub 2019 Feb 24.

1 Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.

Background: Invasion of the central nervous system by haematological malignancies is diagnosed by cytological analyses of cerebrospinal fluid or diagnostic imaging, while quantitative biomarkers for central nervous system invasion are not available and needed to be developed.

Methods: In this study, we measured the concentrations of autotaxin and soluble IL-2 receptor in cerebrospinal fluid and evaluated their usefulness as biomarkers for central nervous system invasion.

Results: We observed that both the autotaxin and soluble IL-2 receptor concentrations in cerebrospinal fluid were higher in subjects with central nervous system invasion than in those without, and the cerebrospinal fluid concentrations were independent from the serum concentrations of these biomarkers. Read More

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http://dx.doi.org/10.1177/0004563218818197DOI Listing
March 2019
5 Reads

A retrospective pilot study to examine the potential of aspartate aminotransferase to alanine aminotransferase ratio as a predictor of postoperative acute kidney injury in patients with hepatocellular carcinoma.

Ann Clin Biochem 2019 Feb 27:4563218817797. Epub 2019 Feb 27.

1 Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio (AST/ALT) is an independent predictor of hepatic disease.

Objectives: To evaluate the association between preoperative AST/ALT and postoperative acute kidney injury in hepatocellular carcinoma patients.

Methods: A total of 422 hepatitis B- or C- virus-associated hepatocellular carcinoma patients, who underwent hepatectomy between September 2012 and April 2018, were enrolled this retrospective study. Read More

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http://dx.doi.org/10.1177/0004563218817797DOI Listing
February 2019
20 Reads
2.080 Impact Factor

Bile acids and the metabolic syndrome.

Ann Clin Biochem 2019 Mar 4:4563218817798. Epub 2019 Mar 4.

Division of Diabetes, Endocrinology and Metabolism, Imperial College London, London, UK.

Bile acids have important roles in the regulation of lipid, glucose and energy metabolism. Metabolic diseases linked to obesity, including type 2 diabetes mellitus and non-alcoholic fatty liver disease, are associated with dysregulation of bile acid homeostasis. Here, the basic chemistry and regulation of bile acids as well as their metabolic effects will be reviewed. Read More

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http://dx.doi.org/10.1177/0004563218817798DOI Listing
March 2019
14 Reads

Retrospective evaluation of a local protocol used to enhance laboratory savings through minimizing the performance of alkaline phosphatase isoenzyme analysis.

Ann Clin Biochem 2019 Mar 13;56(2):298-301. Epub 2018 Dec 13.

2 Department of Clinical Biochemistry and Immunology, Laboratory Medicine, Norfolk and Norwich University Hospitals, Norwich, UK.

Background: Alkaline phosphatase isoenzyme analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally derived screening algorithm for alkaline phosphatase isoenzyme requests on the number of alkaline phosphatase isoenzyme analyses performed, laboratory cost and patient care.

Method: A total of 110 alkaline phosphatase isoenzyme analysis requests from the year 2015 were reviewed and subsequent alkaline phosphatase concentrations were monitored over a two-year period, to determine if the decision of performing/not performing alkaline phosphatase isoenzyme analysis, based on the algorithm, had an impact on patient care and laboratory cost. Read More

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http://dx.doi.org/10.1177/0004563218817571DOI Listing
March 2019
13 Reads

Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation.

Ann Clin Biochem 2019 Mar 13;56(2):228-231. Epub 2018 Dec 13.

1 Laboratoire de Biochimie, PhyMedExp, Université de Montpellier, INSERM, CNRS, CHU de Montpellier, Montpellier, France.

Background: The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction.

Methods: CysC in urine was measured by a particle-enhanced nephelometric immunoassay using Siemens reagents and by a particle-enhanced turbidimetric immunoassay using DiaSys reagents. Read More

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http://dx.doi.org/10.1177/0004563218816341DOI Listing
March 2019
10 Reads

U6 as a microRNA normalizer in serum of patients with hepatocellular carcinoma.

Ann Clin Biochem 2018 Nov 8:4563218808820. Epub 2018 Nov 8.

School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

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http://dx.doi.org/10.1177/0004563218808820DOI Listing
November 2018
2 Reads

A national survey of specimen contamination in the UK.

Ann Clin Biochem 2019 Mar 4;56(2):219-227. Epub 2018 Dec 4.

2 Department of Clinical Biochemistry, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK.

Background: Specimen contamination that goes unnoticed can have many adverse consequences for patients including inappropriate investigations or treatment decisions based on erroneous results. Little is known about UK laboratory practices relating to specimen contamination; therefore, this national survey aimed to gather valuable baseline data.

Methods: An electronic survey consisting of 26 questions was designed to obtain key information relating to specimen contamination including its frequency, how it is identified by laboratories and actions taken in event of confirmed contamination. Read More

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http://dx.doi.org/10.1177/0004563218812500DOI Listing
March 2019
7 Reads

Rapid liquid chromatography tandem mass-spectrometry screening method for urinary metabolites of primary hyperoxaluria.

Ann Clin Biochem 2019 Mar 14;56(2):232-239. Epub 2018 Nov 14.

1 Department of Manual Biochemistry, Health Services Laboratories, London, UK.

Background: The primary hyperoxalurias are inherited disorders of glyoxylate metabolism that lead to overproduction of oxalate, urolithiasis and renal failure. Delays in diagnosis can be costly in terms of preserving renal function. Here we present a rapid liquid chromatography tandem mass-spectrometry screening method for the analysis of metabolites (primary hyperoxaluria metabolites) produced in excess by primary hyperoxaluria patients that include glycolate, glycerate and 2,4-dihydroxyglutarate. Read More

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http://dx.doi.org/10.1177/0004563218811365DOI Listing
March 2019
5 Reads

Peritoneal equilibration test: Does glucose interfere with enzymatic creatinine measurement?

Ann Clin Biochem 2019 Mar 29;56(2):302-303. Epub 2018 Oct 29.

Blood Sciences, New Cross Hospital, Wolverhampton, UK.

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http://journals.sagepub.com/doi/10.1177/0004563218806559
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http://dx.doi.org/10.1177/0004563218806559DOI Listing
March 2019
29 Reads

Clinical utility of chromogranin A for the surveillance of succinate dehydrogenase B- and succinate dehydrogenase D-related paraganglioma.

Ann Clin Biochem 2019 Jan 14;56(1):163-169. Epub 2018 Nov 14.

Department of Diabetes and Endocrinology, Royal Hobart Hospital, School of Medicine, University of Tasmania, Tasmania, Australia.

Background: Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a population-based SDHx sample. Read More

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http://dx.doi.org/10.1177/0004563218811865DOI Listing
January 2019
3 Reads

A rapid and sensitive fluorescence method for detecting urine formaldehyde in patients with Alzheimer's disease.

Ann Clin Biochem 2019 Mar 19;56(2):210-218. Epub 2018 Nov 19.

1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.

Background: Morning urine formaldehyde concentrations could predict the severe degree of dementia in patients with post-stroke dementia and Alzheimer's disease. However, the routinely available technique of high-performance liquid chromatography (HPLC) for detecting urine formaldehyde requires expensive and sophisticated equipment.

Methods: We established a fluorescence spectrophotometric method by using a formaldehyde-specific fluorescent probe-NaFA (λex/em = 430/543 nm). Read More

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http://dx.doi.org/10.1177/0004563218812986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415487PMC
March 2019
7 Reads

Establishment of glycated albumin unit conversion equation from the standardized value (mmol/mol) to the routinely used value (%).

Ann Clin Biochem 2019 Mar 17;56(2):204-209. Epub 2018 Nov 17.

2 Central Clinical Laboratory, Tokyo Women's Medical University Hospital, Tokyo, Japan.

Background: To promote glycated albumin standardization, the Committee on Diabetes Mellitus Indices, Japan Society of Clinical Chemistry published the recommended reference method and is supplying the reference standard material (JCCRM 611). In this study, we evaluated the basic performance of 'standardized enzymatic method' that is traceable to JCCRM 611 and established a unit conversion formula from standardized glycated albumin value (mmol/mol) to the routinely used glycated albumin value (%).

Methods: To evaluate the accuracy and within-run reproducibility of the 'standardized enzymatic method', JCCRM 611 was measured 20 times. Read More

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http://dx.doi.org/10.1177/0004563218808325DOI Listing
March 2019
7 Reads

Using the variance function to generalize Bland-Altman analysis.

Authors:
William A Sadler

Ann Clin Biochem 2019 Mar 29;56(2):198-203. Epub 2018 Oct 29.

Retired, formerly at: Nuclear Medicine Department, Christchurch Hospital, Christchurch, New Zealand.

Background: Bland-Altman analysis is a popular and widely used method for assessing the level of agreement between two analytical methods. An important assumption is that paired method differences exhibit approximately constant (homogeneous) scatter when plotted against pair means. This allows estimation of limits of agreement which retain validity across the entire range of mean values. Read More

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http://dx.doi.org/10.1177/0004563218806560DOI Listing
March 2019
3 Reads

Big data in clinical biochemistry.

Ann Clin Biochem 2018 Sep 12:4563218800735. Epub 2018 Sep 12.

2 Leeds General Infirmary, Leeds, UK.

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http://dx.doi.org/10.1177/0004563218800735DOI Listing
September 2018
2 Reads

Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry.

Ann Clin Biochem 2019 Mar 23;56(2):190-197. Epub 2018 Sep 23.

1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Background: Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited.

Methods: Blood samples from human subjects ( n = 547, male/female = 246/301, age 58. Read More

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http://dx.doi.org/10.1177/0004563218801393DOI Listing
March 2019
3 Reads
2.080 Impact Factor

Circulating tumour DNA as a cancer biomarker.

Authors:
Michael J Duffy

Ann Clin Biochem 2019 Jan 5;56(1):42-48. Epub 2018 Sep 5.

1 UCD School of Medicine, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.

Measurement of genetically altered DNA shed from tumours into the circulation can potentially provide a new generation of blood-based cancer biomarkers. Compared with tissue DNA biomarkers which require surgery or biopsy, samples for circulating tumour DNA assays can be obtained with minimal inconvenience and at lower cost. Furthermore, in contrast to tissue, the use of circulating tumour DNA allows serial monitoring, faster delivery of results and potentially provides an integrative representation of genetic alterations across all tumour sites within a patient. Read More

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http://dx.doi.org/10.1177/0004563218798401DOI Listing
January 2019
15 Reads

Relationship between serum thyrotropin and urine albumin excretion in euthyroid subjects with diabetes.

Ann Clin Biochem 2019 Jan 11;56(1):155-162. Epub 2018 Sep 11.

1 Department of Endocrinology, Prince Charles Hospital, Cwm Taf University Health Board, Merthyr Tydfil, UK.

Background: Microalbuminuria represents vascular and endothelial dysfunction. Thyroid hormones can influence urine albumin excretion as it exerts crucial effects on the kidney and on the vascular system. This study explores the relationship between serum thyrotropin and urine albumin excretion in euthyroid patients with diabetes. Read More

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http://dx.doi.org/10.1177/0004563218797979DOI Listing
January 2019
18 Reads

Carnosine supplementation does not affect serum concentrations of advanced glycation and precursors of lipoxidation end products in autism: a randomized controlled clinical trial.

Ann Clin Biochem 2019 Jan 6;56(1):148-154. Epub 2018 Sep 6.

Department of Nutrition, Tabriz University of Medical Sciences, Tabriz, I. R. Iran.

Background: Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipeptide with antiglycation effects. Read More

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http://dx.doi.org/10.1177/0004563218796860DOI Listing
January 2019
3 Reads

Elevated serum cytokeratin-18 concentration in patients with type 2 diabetes mellitus and non-alcoholic fatty liver disease.

Ann Clin Biochem 2019 Jan 27;56(1):141-147. Epub 2018 Aug 27.

1 Lee's Endocrinology Clinic, Pingtung, Taiwan.

Background: Serum cytokeratin-18 is believed to be a marker of hepatic cell damage. However, few studies have discussed about the serum cytokeratin-18 concentration in type 2 diabetes mellitus patients and investigated its association with non-alcoholic fatty liver disease as well as metabolic biomarkers.

Methods: Healthy participants and type 2 diabetes mellitus patients were enrolled. Read More

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http://dx.doi.org/10.1177/0004563218796259DOI Listing
January 2019
8 Reads

Roche serum folate assay restandardization: an estimate of the new reference interval.

Ann Clin Biochem 2019 Jan 8;56(1):183-184. Epub 2018 Aug 8.

2 Clinical Biochemistry, West Suffolk NHS Foundation Trust, Bury St Edmunds, UK.

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http://dx.doi.org/10.1177/0004563218793159DOI Listing
January 2019
4 Reads

25-Hydroxyvitamin D - Should labs be measuring it?

Ann Clin Biochem 2019 Mar 27;56(2):188-189. Epub 2018 Aug 27.

2 Department of Medicine, University of Auckland, Auckland, New Zealand.

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http://dx.doi.org/10.1177/0004563218796858DOI Listing
March 2019
3 Reads

Quantification of urinary sodium concentrations in term infants.

Ann Clin Biochem 2019 Mar 21;56(2):295-297. Epub 2018 Aug 21.

1 Department of Surgical Paediatrics, Royal Hospital for Children, Glasgow, UK.

Background: Infants with gastrointestinal conditions and poor weight gain are administered sodium supplementation based on urinary sodium concentrations. However, the reference range of urinary electrolytes is unknown. The aim of this study was to ascertain the normal values of urinary electrolytes in healthy, term infants. Read More

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http://journals.sagepub.com/doi/10.1177/0004563218796592
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http://dx.doi.org/10.1177/0004563218796592DOI Listing
March 2019
7 Reads

A two-step homogeneous assay for apolipoprotein E-containing high-density lipoprotein-cholesterol.

Ann Clin Biochem 2019 Jan 16;56(1):123-132. Epub 2018 Aug 16.

1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Background: Apolipoprotein E-containing high-density lipoprotein shows antiatherogenic properties in vitro. There is a need for a homogeneous assay to determine the concentration of apolipoprotein E-containing high-density lipoprotein for in vivo studies.

Methods: In the proposed homogeneous assay, lipoproteins other than apolipoprotein E-containing high-density lipoprotein were eliminated in the first step. Read More

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http://dx.doi.org/10.1177/0004563218795212DOI Listing
January 2019
16 Reads