4,667 results match your criteria Annals of clinical biochemistry[Journal]


ANNALS EXPRESS: The association of serum and vitreous adropin concentrations with diabetic retinopathy.

Ann Clin Biochem 2018 Dec 4:4563218820359. Epub 2018 Dec 4.

Qilu Hospital of Shandong University (Qingdao).

Objective: Adropin, a newly identified regulatory protein encoded by Enho gene, is correlated with insulin sensitivity and diabetes. The aim of this study is to determine whether serum and vitreous adropin concentrations are correlated with the presence of diabetic retinopathy (DR).

Methods: A population of 165 patients with type 2 diabetes mellitus (T2DM) [52 without DR, 69 with non-proliferative diabetic retinopathy (NPDR), and 44 patients with proliferative diabetic retinopathy (PDR)] was enrolled in this study. Read More

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http://dx.doi.org/10.1177/0004563218820359DOI Listing
December 2018
2 Reads

ANNALS EXPRESS: Hematopoietic alterations in neonates with congenital diaphragmatic hernia receiving extracorporeal membrane oxygenation support.

Ann Clin Biochem 2018 Dec 4:4563218820052. Epub 2018 Dec 4.

Department of Neonatology and Pediatric Critical Care Medicine, University of Bonn Children's Hospital.

Background Extracorporeal membrane oxygenation procedure (ECMO) has been established in the therapy of respiratory insufficient infants with congenital diaphragmatic hernia (CDH). In CDH newborns, a delay in transfer to an ECMO center is associated with a sharp increase in mortality. Predictive factors for ECMO support are urgently needed. Read More

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http://dx.doi.org/10.1177/0004563218820052DOI Listing
December 2018
3 Reads

ANNALS EXPRESS: Autotaxin and sIL-2R levels in cerebrospinal fluids are useful for the diagnosis of central nervous system invasion caused by hematological malignancies.

Ann Clin Biochem 2018 Dec 4:4563218818197. Epub 2018 Dec 4.

The University of Tokyo.

Background: Invasion of the central nervous system (CNS) by hematological malignancies is diagnosed by cytological analyses of cerebrospinal fluid (CSF) or diagnostic imaging, while quantitative biomarkers for CNS invasion are not available and needed to be developed. Methods; In this study, we measured the levels of autotaxin (ATX) and soluble IL-2 receptor (sIL-2R) in cerebrospinal fluid (CSF) and evaluated their usefulness as biomarkers for CNS invasion.

Results: We observed that both the ATX and sIL-2R levels in CSF were higher in subjects with CNS invasion than in those without, and the CSF levels were independent from the serum levels of these biomarkers. Read More

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http://dx.doi.org/10.1177/0004563218818197DOI Listing
December 2018
1 Read

ANNALS EXPRESS: A retrospective pilot study to examine the potential of aspartate aminotransferase to alanine aminotransferase ratio as a predictor of post-operative acute kidney injury in patients with hepatocellular carcinoma.

Ann Clin Biochem 2018 Nov 19:4563218817797. Epub 2018 Nov 19.

Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology.

Background: The aspartate aminotransferase (AST) / alanine aminotransferase (ALT) ratio(AST/ALT) is an independent predictor of hepatic disease.

Objectives: To evaluate the association between pre-operative AST/ALT and post-operative acute kidney injury(AKI) in hepatocellular carcinoma (HCC) patients.

Methods: A total of 422 hepatitis B- or C- virus associated HCC patients, who underwent hepatectomy between September 2012 and April 2018, were enrolled this retrospective study. Read More

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http://dx.doi.org/10.1177/0004563218817797DOI Listing
November 2018
6 Reads
2.080 Impact Factor

ANNALS EXPRESS: Bile acids and the metabolic syndrome.

Ann Clin Biochem 2018 Nov 19:4563218817798. Epub 2018 Nov 19.

Imperial College London.

Bile acids have important roles in the regulation of lipid, glucose and energy metabolism. Metabolic diseases linked to obesity, including type 2 diabetes mellitus and non-alcoholic fatty liver disease, are associated with dysregulation of bile acid homeostasis. Here basic chemistry and regulation of bile acids will be reviewed, as well as their metabolic effects. Read More

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http://journals.sagepub.com/doi/10.1177/0004563218817798
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http://dx.doi.org/10.1177/0004563218817798DOI Listing
November 2018
3 Reads

ANNALS EXPRESS: Retrospective evaluation of a local protocol used to enhance laboratory savings through minimising the performance of alkaline phosphatase isoenzyme analysis.

Ann Clin Biochem 2018 Nov 17:4563218817571. Epub 2018 Nov 17.

Norfolk and Norwich University Hospitals NHS Foundation Trust.

Background: Alkaline phosphatase (ALP) isoenzyme analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally-derived screening algorithm for ALP isoenzyme requests on the number of ALP isoenzyme analyses performed, laboratory cost, and patient care.

Method: 110 ALP isoenzyme analysis requests from the year 2015 were reviewed and subsequent ALP concentrations were monitored over a 2-year period, to determine if the decision of performing/not performing ALP isoenzyme analysis, based on the algorithm, had an impact on patient care and laboratory cost. Read More

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http://dx.doi.org/10.1177/0004563218817571DOI Listing
November 2018
4 Reads

ANNALS EXPRESS: Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation.

Ann Clin Biochem 2018 Nov 14:4563218816341. Epub 2018 Nov 14.

PhyMedExp, Université de Montpellier, INSERM, CNRS, CHU de Montpellier.

Background: The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction.

Methods: CysC in urine was measured by a particle enhanced nephelometric immunoassay (PENIA) using Siemens reagents and by a particle enhanced turbidimetric immunoassay (PENIA) using DiaSys reagents. Read More

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http://dx.doi.org/10.1177/0004563218816341DOI Listing
November 2018
6 Reads

U6 as a microRNA normalizer in serum of patients with hepatocellular carcinoma.

Ann Clin Biochem 2018 Nov 8:4563218808820. Epub 2018 Nov 8.

School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

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http://dx.doi.org/10.1177/0004563218808820DOI Listing
November 2018
1 Read

ANNALS EXPRESS: A national survey of specimen contamination in the UK.

Ann Clin Biochem 2018 Oct 29:4563218812500. Epub 2018 Oct 29.

Central Manchester University Hospitals NHS Foundation Trust.

Background: Specimen contamination that goes unnoticed can have many adverse consequences for patients including inappropriate investigations or treatment decisions based on erroneous results. Little is known about UK laboratory practices relating to specimen contamination therefore this national survey aimed to gather valuable baseline data.

Methods: An electronic survey consisting of 26 questions was designed to obtain key information relating to specimen contamination including its frequency, how it is indentified by laboratories, and actions taken in event of confirmed contamination. Read More

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http://dx.doi.org/10.1177/0004563218812500DOI Listing
October 2018
2 Reads

Rapid liquid chromatography tandem mass-spectrometry screening method for urinary metabolites of primary hyperoxaluria.

Ann Clin Biochem 2018 Nov 14:4563218811365. Epub 2018 Nov 14.

1 Department of Manual Biochemistry, Health Services Laboratories, London, UK.

Background: The primary hyperoxalurias are inherited disorders of glyoxylate metabolism that lead to overproduction of oxalate, urolithiasis and renal failure. Delays in diagnosis can be costly in terms of preserving renal function. Here we present a rapid liquid chromatography tandem mass-spectrometry screening method for the analysis of metabolites (primary hyperoxaluria metabolites) produced in excess by primary hyperoxaluria patients that include glycolate, glycerate and 2,4-dihydroxyglutarate. Read More

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http://dx.doi.org/10.1177/0004563218811365DOI Listing
November 2018
3 Reads

Peritoneal equilibration test: Does glucose interfere with enzymatic creatinine measurement?

Ann Clin Biochem 2018 Oct 29:4563218806559. Epub 2018 Oct 29.

Blood Sciences, New Cross Hospital, Wolverhampton, UK.

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http://dx.doi.org/10.1177/0004563218806559DOI Listing
October 2018
1 Read

Clinical utility of chromogranin A for the surveillance of succinate dehydrogenase B- and succinate dehydrogenase D-related paraganglioma.

Ann Clin Biochem 2018 Nov 14:4563218811865. Epub 2018 Nov 14.

Department of Diabetes and Endocrinology, Royal Hobart Hospital, School of Medicine, University of Tasmania, Tasmania, Australia.

Background: Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a population-based SDHx sample. Read More

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http://dx.doi.org/10.1177/0004563218811865DOI Listing
November 2018
2 Reads

A rapid and sensitive fluorescence method for detecting urine formaldehyde in patients with Alzheimer's disease.

Ann Clin Biochem 2018 Nov 19:4563218812986. Epub 2018 Nov 19.

1 Laboratory of Alzheimer's Optoelectric Therapy, Alzheimer's Disease Center, Beijing Institute of Brain Disorders, Center for Brain Disorders Research, Capital Medical University, Beijing, China.

Background: Morning urine formaldehyde concentrations could predict the severe degree of dementia in patients with post-stroke dementia and Alzheimer's disease. However, the routinely available technique of high-performance liquid chromatography (HPLC) for detecting urine formaldehyde requires expensive and sophisticated equipment.

Methods: We established a fluorescence spectrophotometric method by using a formaldehyde-specific fluorescent probe-NaFA (λex/em = 430/543 nm). Read More

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http://dx.doi.org/10.1177/0004563218812986DOI Listing
November 2018
3 Reads

Establishment of glycated albumin unit conversion equation from the standardized value (mmol/mol) to the routinely used value (%).

Ann Clin Biochem 2018 Nov 17:4563218808325. Epub 2018 Nov 17.

2 Central Clinical Laboratory, Tokyo Women's Medical University Hospital, Tokyo, Japan.

Background: To promote glycated albumin standardization, the Committee on Diabetes Mellitus Indices, Japan Society of Clinical Chemistry published the recommended reference method and is supplying the reference standard material (JCCRM 611). In this study, we evaluated the basic performance of 'standardized enzymatic method' that is traceable to JCCRM 611 and established a unit conversion formula from standardized glycated albumin value (mmol/mol) to the routinely used glycated albumin value (%).

Methods: To evaluate the accuracy and within-run reproducibility of the 'standardized enzymatic method', JCCRM 611 was measured 20 times. Read More

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http://dx.doi.org/10.1177/0004563218808325DOI Listing
November 2018
3 Reads

Using the variance function to generalize Bland-Altman analysis.

Authors:
William A Sadler

Ann Clin Biochem 2018 Oct 29:4563218806560. Epub 2018 Oct 29.

Retired, formerly at: Nuclear Medicine Department, Christchurch Hospital, Christchurch, New Zealand.

Background: Bland-Altman analysis is a popular and widely used method for assessing the level of agreement between two analytical methods. An important assumption is that paired method differences exhibit approximately constant (homogeneous) scatter when plotted against pair means. This allows estimation of limits of agreement which retain validity across the entire range of mean values. Read More

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http://dx.doi.org/10.1177/0004563218806560DOI Listing
October 2018
2 Reads

Big data in clinical biochemistry.

Ann Clin Biochem 2018 Sep 12:4563218800735. Epub 2018 Sep 12.

2 Leeds General Infirmary, Leeds, UK.

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http://dx.doi.org/10.1177/0004563218800735DOI Listing
September 2018

Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry.

Ann Clin Biochem 2018 Sep 23:4563218801393. Epub 2018 Sep 23.

1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Background Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. Methods Blood samples from human subjects ( n = 547, male/female = 246/301, age 58. Read More

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http://dx.doi.org/10.1177/0004563218801393DOI Listing
September 2018
1 Read
2.080 Impact Factor

Circulating tumour DNA as a cancer biomarker.

Authors:
Michael J Duffy

Ann Clin Biochem 2018 Sep 5:4563218798401. Epub 2018 Sep 5.

1 UCD School of Medicine, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.

Measurement of genetically altered DNA shed from tumours into the circulation can potentially provide a new generation of blood-based cancer biomarkers. Compared with tissue DNA biomarkers which require surgery or biopsy, samples for circulating tumour DNA assays can be obtained with minimal inconvenience and at lower cost. Furthermore, in contrast to tissue, the use of circulating tumour DNA allows serial monitoring, faster delivery of results and potentially provides an integrative representation of genetic alterations across all tumour sites within a patient. Read More

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http://dx.doi.org/10.1177/0004563218798401DOI Listing
September 2018
14 Reads

Relationship between serum thyrotropin and urine albumin excretion in euthyroid subjects with diabetes.

Ann Clin Biochem 2019 Jan 11;56(1):155-162. Epub 2018 Sep 11.

1 Department of Endocrinology, Prince Charles Hospital, Cwm Taf University Health Board, Merthyr Tydfil, UK.

Background: Microalbuminuria represents vascular and endothelial dysfunction. Thyroid hormones can influence urine albumin excretion as it exerts crucial effects on the kidney and on the vascular system. This study explores the relationship between serum thyrotropin and urine albumin excretion in euthyroid patients with diabetes. Read More

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http://dx.doi.org/10.1177/0004563218797979DOI Listing
January 2019
10 Reads

Carnosine supplementation does not affect serum concentrations of advanced glycation and precursors of lipoxidation end products in autism: a randomized controlled clinical trial.

Ann Clin Biochem 2018 Sep 6:4563218796860. Epub 2018 Sep 6.

Department of Nutrition, Tabriz University of Medical Sciences, Tabriz, I. R. Iran.

Background Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipeptide with antiglycation effects. Read More

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http://dx.doi.org/10.1177/0004563218796860DOI Listing
September 2018
2 Reads

Elevated serum cytokeratin-18 concentration in patients with type 2 diabetes mellitus and non-alcoholic fatty liver disease.

Ann Clin Biochem 2019 Jan 27;56(1):141-147. Epub 2018 Aug 27.

1 Lee's Endocrinology Clinic, Pingtung, Taiwan.

Background: Serum cytokeratin-18 is believed to be a marker of hepatic cell damage. However, few studies have discussed about the serum cytokeratin-18 concentration in type 2 diabetes mellitus patients and investigated its association with non-alcoholic fatty liver disease as well as metabolic biomarkers.

Methods: Healthy participants and type 2 diabetes mellitus patients were enrolled. Read More

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http://dx.doi.org/10.1177/0004563218796259DOI Listing
January 2019
6 Reads

Roche serum folate assay restandardization: an estimate of the new reference interval.

Ann Clin Biochem 2018 Aug 8:4563218793159. Epub 2018 Aug 8.

2 Clinical Biochemistry, West Suffolk NHS Foundation Trust, Bury St Edmunds, UK.

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http://dx.doi.org/10.1177/0004563218793159DOI Listing
August 2018
3 Reads

25-Hydroxyvitamin D - Should labs be measuring it?

Ann Clin Biochem 2018 Aug 27:4563218796858. Epub 2018 Aug 27.

2 Department of Medicine, University of Auckland, Auckland, New Zealand.

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http://dx.doi.org/10.1177/0004563218796858DOI Listing
August 2018
2 Reads

Quantification of urinary sodium concentrations in term infants.

Ann Clin Biochem 2018 Aug 21:4563218796592. Epub 2018 Aug 21.

1 Department of Surgical Paediatrics, Royal Hospital for Children, Glasgow, UK.

Background Infants with gastrointestinal conditions and poor weight gain are administered sodium supplementation based on urinary sodium concentrations. However, the reference range of urinary electrolytes is unknown. The aim of this study was to ascertain the normal values of urinary electrolytes in healthy, term infants. Read More

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http://journals.sagepub.com/doi/10.1177/0004563218796592
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http://dx.doi.org/10.1177/0004563218796592DOI Listing
August 2018
5 Reads

A two-step homogeneous assay for apolipoprotein E-containing high-density lipoprotein-cholesterol.

Ann Clin Biochem 2018 Aug 16:4563218795212. Epub 2018 Aug 16.

1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Background Apolipoprotein E-containing high-density lipoprotein shows antiatherogenic properties in vitro. There is a need for a homogeneous assay to determine the concentration of apolipoprotein E-containing high-density lipoprotein for in vivo studies. Methods In the proposed homogeneous assay, lipoproteins other than apolipoprotein E-containing high-density lipoprotein were eliminated in the first step. Read More

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http://dx.doi.org/10.1177/0004563218795212DOI Listing
August 2018
11 Reads

Estimation of the prevalence of cholesteryl ester storage disorder in a cohort of patients with clinical features of familial hypercholesterolaemia.

Ann Clin Biochem 2018 Aug 8:4563218793165. Epub 2018 Aug 8.

1 FH Wales Research Team, Cardiff University, Wales Heart Research Institute, Cardiff, UK.

Background and aim Familial hypercholesterolaemia is caused by variants in the low-density lipoprotein cholesterol metabolic pathway involving LDLR, APOB and PCSK9 genes. A national genetic testing service in Wales, UK has observed that no familial hypercholesterolaemia variant is found in almost 80% patients with the familial hypercholesterolaemia phenotype. It has recently been suggested that some adult patients with a familial hypercholesterolaemia phenotype may have cholesteryl ester storage disease which can also present as a mixed hyperlipidaemia. Read More

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http://dx.doi.org/10.1177/0004563218793165DOI Listing
August 2018
3 Reads

Method for measurement of serum copper, zinc and selenium using total reflection X-ray fluorescence spectroscopy on the PICOFOX analyser: Validation and comparison with atomic absorption spectroscopy and inductively coupled plasma mass spectrometry.

Ann Clin Biochem 2018 Aug 16:4563218793163. Epub 2018 Aug 16.

1 Derriford Combined Laboratory, Derriford Hospital, Plymouth, UK.

Background Total reflection X-ray fluorescence is a comparably new method for the measurement of trace elements in biological samples. Methods Results obtained by total reflection X-ray fluorescence were compared to atomic absorption spectroscopy for Cu and Zn and inductively coupled plasma mass spectrometry for Cu, Zn and Se in patient serum. The total reflection X-ray fluorescence assay was characterized for accuracy; recovery; intra- and inter-assay imprecision (using patients' samples, external quality assurance and quality control materials); limit of blank; limit of detection; linearity; interference and stability of prepared samples. Read More

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http://dx.doi.org/10.1177/0004563218793163DOI Listing
August 2018
2 Reads

Reflective molecular testing for myeloproliferative neoplasms in patients with elevated serum vitamin B.

Ann Clin Biochem 2018 Nov 30;55(6):717-718. Epub 2018 Jul 30.

Cancer Molecular Diagnostics, St. James's Hospital, Dublin, Ireland.

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http://dx.doi.org/10.1177/0004563218790986DOI Listing
November 2018
2 Reads

The combination of cerebrospinal fluid procalcitonin, lactate, interleukin-8 and interleukin-10 concentrations for the diagnosis of postneurosurgical bacterial meningitis: A prospective study.

Ann Clin Biochem 2018 Aug 27:4563218794729. Epub 2018 Aug 27.

3 Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

Background The differential diagnosis between postneurosurgical bacterial meningitis and aseptic meningitis remains challenging both for the clinician and the laboratory. Combinations of markers, as opposed to single ones, may improve diagnosis and thereby survival. Methods This prospective cohort study included patients with suspected bacterial meningitis after neurosurgery. Read More

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http://dx.doi.org/10.1177/0004563218794729DOI Listing
August 2018
11 Reads

Falsely decreased ferritin concentrations in two patients with haemophagocytic lymphohistiocytosis: A case report.

Ann Clin Biochem 2018 Aug 9:4563218793166. Epub 2018 Aug 9.

1 Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, The Netherlands.

The high-dose hook effect, or prozone effect, can lead to negative or falsely lowered plasma ferritin results. Here, cases of a 16-year-old boy and a 70-year-old woman with haemophagocytic lymphohystiocytosis with extremely high concentrations of plasma ferritin (387,000  μg/L and 138,000  μg/L, respectively) are presented. In both cases, falsely lowered ferritin results were reported without any analyser flag. Read More

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http://dx.doi.org/10.1177/0004563218793166DOI Listing
August 2018
3 Reads

The concentration of total sialic acid in chronic hepatitis B and C.

Ann Clin Biochem 2018 Aug 16:4563218792292. Epub 2018 Aug 16.

1 Department of Biochemical Diagnostics, Medical University of Bialystok, Bialystok, Poland.

Background The synthesis and glycosylation of glycoproteins and glycolipids take place in the liver. Thus, liver diseases may affect serum concentrations of some carbohydrate derivatives, especially the concentration of sialic acid which is attached to the end of oligosaccharide chains. The aim of this study was to measure and compare the serum concentration of total sialic acid in chronic hepatitis B and C. Read More

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http://journals.sagepub.com/doi/10.1177/0004563218792292
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http://dx.doi.org/10.1177/0004563218792292DOI Listing
August 2018
18 Reads

Prospective analysis of phoned potassium results and what general practitioners do with them: a pilot study.

Ann Clin Biochem 2018 Nov 2;55(6):710-712. Epub 2018 Aug 2.

Department of Biochemical Medicine, Ninewells Hospital & Medical School, Dundee, UK.

Background Communicating abnormal results to requesting clinicians is an essential part of clinical authorisation. Guidance from the Royal College of Pathologists on communication of critical/unexpected results is issued as 'advice to pathologists'. The 2017 guidelines advise rapid communication of serum potassium results ≤2. Read More

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http://dx.doi.org/10.1177/0004563218790298DOI Listing
November 2018
3 Reads

Elevated serum YKL-40 correlates with clinical characteristics in patients with polymyositis or dermatomyositis.

Ann Clin Biochem 2018 Jan 1:4563218786979. Epub 2018 Jan 1.

3 Department of Clinical Immunology, Institution for Laboratory Medicine, Changshu, China.

Background Serum YKL-40 has been proved to be a promising biomarker for estimating the disease activity of several autoimmune diseases. However, its utility in polymyositis or dermatomyositis has not been established. The aim of this study was to investigate the utility of YKL-40 in patients with polymyositis/dermatomyositis. Read More

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http://dx.doi.org/10.1177/0004563218786979DOI Listing
January 2018
5 Reads

The effects of cigarette smoking and smoking cessation on high-density lipoprotein functions: implications for coronary artery disease.

Ann Clin Biochem 2018 Aug 1:4563218788386. Epub 2018 Aug 1.

Department of Cardiology, Peking Union Medical College Hospital (PUMCH), Beijing, P. R. China.

Background Smoking cessation was associated with improved prognosis of coronary artery disease. This study was designed to investigate the effect of smoking cessation on high-density lipoprotein functionality in coronary artery disease patients. Methods In this prospective, randomized and parallel controlled study, coronary artery disease smokers ( n = 28) and healthy smokers ( n = 30) were divided into smoking cessation group and continuous smoking group, respectively. Read More

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http://dx.doi.org/10.1177/0004563218788386DOI Listing
August 2018
15 Reads

Does thyrotoxic periodic paralysis have a genetic predisposition? A case report.

Ann Clin Biochem 2018 Nov 20;55(6):713-716. Epub 2018 Jul 20.

1 Department of Clinical Chemistry, Tallaght University Hospital, Tallaght, Dublin, Ireland.

Thyrotoxic periodic paralysis is a rare complication of hyperthyroidism where increased influx of potassium into skeletal muscle cells leads to profound hypokalaemia and paralysis. Most cases arise sporadically in Asians; however, it is being increasingly reported in Caucasians. It is regarded as a channelopathy where a genetic and/or acquired defect in the sodium-potassium (Na/K-ATPase) pump renders it more sensitive to excess thyroid hormone in susceptible individuals. Read More

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http://dx.doi.org/10.1177/0004563218785395DOI Listing
November 2018
27 Reads

Improved testing for vitamin B deficiency: correcting MMA for eGFR reduces the number of patients classified as vitamin B deficient.

Ann Clin Biochem 2018 Nov 6;55(6):685-692. Epub 2018 Jun 6.

1 Department of Clinical Chemistry, Catharina Hospital, Eindhoven, The Netherlands.

Background Methylmalonic acid (MMA) can detect functional vitamin B deficiencies as it accumulates early when intracellular deficits arise. However, impaired clearance of MMA from blood due to decreased glomerular filtration rate (eGFR) also results in elevated plasma MMA concentrations. Alternative to clinical trials, a data mining approach was chosen to quantify and compensate for the effect of decreased eGFR on MMA concentration. Read More

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http://dx.doi.org/10.1177/0004563218778300DOI Listing
November 2018
4 Reads

Hypomagnesaemia is absent in children with autosomal dominant polycystic kidney disease.

Ann Clin Biochem 2018 Jan 1:4563218785190. Epub 2018 Jan 1.

3 Department of Paediatrics, Friedrich-Schiller-University Jena, Jena, Germany.

Background Hypomagnesaemia is present in 40-50% of children with autosomal dominant renal cysts and diabetes syndrome (RCAD). On the contrary, the prevalence of hypomagnesaemia in children with autosomal dominant polycystic kidney disease (ADPKD) has never been examined. We aimed to investigate whether hypomagnesaemia is present in children with polycystic kidney diseases. Read More

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http://dx.doi.org/10.1177/0004563218785190DOI Listing
January 2018
4 Reads

Challenges and opportunities from the revised international consensus on ANCA testing.

Authors:
Stephen Holding

Ann Clin Biochem 2019 Jan 6;56(1):4-6. Epub 2018 Jun 6.

1 Immunology Department, Hull Royal Infirmary, Hull, UK.

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http://dx.doi.org/10.1177/0004563218778303DOI Listing
January 2019
5 Reads

Potential interference in the Abbott Architect 2nd generation total testosterone assay.

Ann Clin Biochem 2018 Sep 6;55(5):621-622. Epub 2018 Jun 6.

Clinical Biochemistry, East Kent Hospitals University NHS Foundation Trust, Ashford, UK.

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http://dx.doi.org/10.1177/0004563218780888DOI Listing
September 2018
23 Reads

Clinically inappropriate post hoc exclusion of study participants from test accuracy calculations: the ROMA score, an example from a recent NICE diagnostic assessment.

Ann Clin Biochem 2018 Jan 1:4563218782722. Epub 2018 Jan 1.

1 Kleijnen Systematic Reviews Ltd, York, UK.

Objective To explore how the definition of the target condition and post hoc exclusion of participants can limit the usefulness of diagnostic accuracy studies. Methods We used data from a systematic review, conducted for a NICE diagnostic assessment of risk scores to inform secondary care decisions about specialist referral for women with suspected ovarian cancer, to explore how the definition of the target condition and post hoc exclusion of participants can limit the usefulness of diagnostic accuracy studies to inform clinical practice. Results Fourteen of the studies evaluated the ROMA score, nine used Abbott ARCHITECT tumour marker assays, five used Roche Elecsys. Read More

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http://journals.sagepub.com/doi/10.1177/0004563218782722
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http://dx.doi.org/10.1177/0004563218782722DOI Listing
January 2018
12 Reads

High-performance liquid chromatography methods for the analysis of endogenous cortisol and cortisone in human urine: comparison of mass spectrometry and fluorescence detection.

Ann Clin Biochem 2018 Jan 1:4563218783789. Epub 2018 Jan 1.

1 Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, Poznań, Poland.

Background The analysis of steroids in biological matrices is challenging. One can apply immunoassay as well as gas and liquid chromatography with various types of detection, depending on the available equipment and the experience of the analyst. The question is how the methods are interchangeable between themselves. Read More

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http://dx.doi.org/10.1177/0004563218783789DOI Listing
January 2018
12 Reads

Too much of a good thing - the over requesting of cardiac troponin.

Authors:
Paul Collinson

Ann Clin Biochem 2018 Sep 30;55(5):522-524. Epub 2018 May 30.

1 Department of Clinical Blood Sciences, St George's University Hospitals NHS Foundation Trust and St George's University of London, Cranmer Terrace, London, UK.

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http://dx.doi.org/10.1177/0004563218776027DOI Listing
September 2018
3 Reads

Therapeutic drug monitoring of tumour necrosis factor inhibitors in the management of chronic inflammatory diseases.

Ann Clin Biochem 2018 Jan 1:4563218782286. Epub 2018 Jan 1.

1 Pharmacy Service, University Hospital of Salamanca, Salamanca, Spain.

Tumour necrosis factor inhibitor therapy has drastically changed the management of chronic inflammatory diseases. Some important drawbacks that can cause loss of response during treatment with these drugs are related to their large individual variability, the disease burden and the formation of antidrug antibodies that increase its clearance. Therapeutic drug monitoring of these drugs is not yet recommended by all scientific societies, and if so, only in patients with inflammatory symptoms. Read More

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http://dx.doi.org/10.1177/0004563218782286DOI Listing
January 2018
2 Reads

A novel high-throughput assay for the measurement of salivary progesterone by liquid chromatography tandem mass spectrometry.

Ann Clin Biochem 2018 Jan 1:4563218780904. Epub 2018 Jan 1.

3 Biochemistry Department, Wythenshawe Hospital, Manchester University NHS Trust, Manchester Academic Health Sciences Centre, Manchester, UK.

Background Liquid chromatography tandem mass spectrometry (LC-MS/MS) enables specific and sensitive quantification of steroids with a high throughput. Saliva sampling is advantageous for multisample profiling over longer periods of time, as it is non-invasive, cheap, can be carried out at home and does not require the attendance of clinical personnel. We developed a rapid LC-MS/MS for the measurement of salivary progesterone, frequently assessed as ovulation marker in patients desiring fertility. Read More

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http://dx.doi.org/10.1177/0004563218780904DOI Listing
January 2018
9 Reads

Phenotyping and relative quantification of vitamin D binding protein in a paediatric population by using liquid chromatography-tandem mass spectrometry.

Ann Clin Biochem 2019 Jan 17;56(1):56-63. Epub 2018 Jun 17.

1 Laboratory of Medicine, Department of Clinical Biochemistry, Hospital Universitario Central de Asturias, Oviedo, Spain.

Background: Adequate concentrations of vitamin D are required to ensure bone health and minimize the incidence of multiple extraskeletal diseases. Although total 25-hydroxyvitamin D (25OHD) remains the recommended biomarker for assessing vitamin D status, it has been speculated that free 25OHD correlates better with clinical outcomes. The calculation of free 25OHD depends on the concentrations of vitamin D binding protein (DBP), the determination of which involves different immunoassays and has led to varying results and conclusions. Read More

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http://dx.doi.org/10.1177/0004563218780135DOI Listing
January 2019
2 Reads

Fast screening of N-glycosylation disorders by sialotransferrin profiling with capillary zone electrophoresis.

Ann Clin Biochem 2018 Nov 11;55(6):693-701. Epub 2018 Jun 11.

Laboratory of Metabolic Diseases, Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Background Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focusing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. Read More

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http://dx.doi.org/10.1177/0004563218779609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196592PMC
November 2018
28 Reads

Renal stones.

Ann Clin Biochem 2019 Jan 13;56(1):15-27. Epub 2018 Jun 13.

2 Department of Urology, Lister Hospital, Stevenage, UK.

Renal stone disease is a worldwide problem which carries significant morbidity. It frequently requires specialist urology intervention. Patients with recurrent disease and those at high risk require specialist investigations and review. Read More

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http://dx.doi.org/10.1177/0004563218781672DOI Listing
January 2019
4 Reads

An assessment of the effect of haemoglobin variants on detection by faecal immunochemical tests.

Ann Clin Biochem 2018 Nov 7;55(6):706-709. Epub 2018 Jun 7.

1 NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK.

Background Faecal immunochemical tests (FIT) for haemoglobin (Hb) are being used in the investigation of colorectal cancer. These tests use antibodies raised to the globin moiety of human Hb. Where the globin structure is abnormal or reduced, it is possible that antibody binding, and thus Hb-detection may be affected. Read More

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http://dx.doi.org/10.1177/0004563218778716DOI Listing
November 2018
4 Reads

Does the mass of sample loaded affect faecal haemoglobin concentration using the faecal immunochemical test?

Ann Clin Biochem 2018 Nov 6;55(6):702-705. Epub 2018 Jun 6.

1 NHS Bowel Cancer Screening Programme, Guildford, UK.

Background Quantitative faecal immunochemical tests (FIT) for haemoglobin are being used for colorectal cancer (CRC) screening for asymptomatic populations and are being indicated as a suitable test to rule out CRC in symptomatic populations. Faecal samples are typically collected by patients using a probe attached to the cap of a device which is inserted into a collection device into the preservative buffer, passing through a collar to remove excess sample: this process has potential for pre-analytical error. This study investigates whether faecal haemoglobin concentration (f-Hb) results are affected by the mass and method of sample collection. Read More

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http://dx.doi.org/10.1177/0004563218778701DOI Listing
November 2018
4 Reads

Establishment of a simpler method for measuring HDL-microRNAs.

Ann Clin Biochem 2018 Jan 1:4563218775770. Epub 2018 Jan 1.

3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.

Background MicroRNAs are present not only in exosomes but also in high-density lipoprotein (HDL) and have the potential as biomarkers for various diseases. Various purification methods have been developed to quantify HDL-miRNAs; however, they are unsuitable for clinical applications. Therefore, we aimed to establish a simpler analytical method to quantify HDL-miRNAs for clinical applications. Read More

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http://dx.doi.org/10.1177/0004563218775770DOI Listing
January 2018
6 Reads