4,633 results match your criteria Annals of clinical biochemistry[Journal]


ANNALS EXPRESS: Does thyrotoxic periodic paralysis have a genetic predisposition? A case report.

Ann Clin Biochem 2018 Jan 1:4563218785395. Epub 2018 Jan 1.

AMNCH Tallaght Hospital.

Thyrotoxic periodic paralysis (TPP) is a rare complication of hyperthyroidism where increased influx of potassium into skeletal muscle cells leads to profound hypokalaemia and paralysis. Most cases arise sporadically in Asians however it is being increasingly reported in Caucasians. TPP is regarded as a channelopathy where a genetic and/or acquired defect in the sodium-potassium (Na+/K+-ATPase) pump renders it more sensitive to excess thyroid hormone in susceptible individuals. Read More

View Article
January 2018
2 Reads

Improved testing for vitamin B deficiency: correcting MMA for eGFR reduces the number of patients classified as vitamin B deficient.

Ann Clin Biochem 2018 Jan 1:4563218778300. Epub 2018 Jan 1.

1 Department of Clinical Chemistry, Catharina Hospital, Eindhoven, The Netherlands.

Background Methylmalonic acid (MMA) can detect functional vitamin B deficiencies as it accumulates early when intracellular deficits arise. However, impaired clearance of MMA from blood due to decreased glomerular filtration rate (eGFR) also results in elevated plasma MMA concentrations. Alternative to clinical trials, a data mining approach was chosen to quantify and compensate for the effect of decreased eGFR on MMA concentration. Read More

View Article
January 2018

ANNALS EXPRESS: Hypomagnesaemia is absent in children with autosomal dominant polycystic kidney disease.

Ann Clin Biochem 2018 Jan 1:4563218785190. Epub 2018 Jan 1.

Background: Hypomagnesaemia is present in 40-50% of children with autosomal dominant renal cysts and diabetes syndrome (RCAD). On contrary, the prevalence of hypomagnesaemia in children with autosomal dominant polycystic kidney disease (ADPKD) has never been examined. We aimed to investigate whether hypomagnesaemia is present in children with polycystic kidney diseases. Read More

View Article
January 2018

Challenges and opportunities from the revised international consensus on ANCA testing.

Authors:
Stephen Holding

Ann Clin Biochem 2018 Jan 1:4563218778303. Epub 2018 Jan 1.

1 Immunology Department, Hull Royal Infirmary, Hull, UK.

View Article
January 2018

Potential interference in the Abbott Architect 2nd generation total testosterone assay.

Ann Clin Biochem 2018 Jan 1:4563218780888. Epub 2018 Jan 1.

Clinical Biochemistry, East Kent Hospitals University NHS Foundation Trust, Ashford, UK.

View Article
January 2018

ANNALS EXPRESS: Clinically inappropriate post-hoc exclusion of study participants from test accuracy calculations: The ROMA score, an example from a recent NICE diagnostic assessment.

Ann Clin Biochem 2018 Jan 1:4563218782722. Epub 2018 Jan 1.

Kleijnen Systematic Reviews Ltd.

Objective To explore how the definition of the target condition and post- hoc exclusion of participants can limit the usefulness of diagnostic accuracy studies. Methods We used data from a systematic review, conducted for a NICE diagnostic assessment of risk scores to inform secondary care decisions about specialist referral for women with suspected ovarian cancer, to explore how the definition of the target condition and post- hoc exclusion of participants can limit the usefulness of diagnostic accuracy studies to inform clinical practice. Results Fourteen of the studies evaluated the ROMA score, nine used Abbott ARCHITECT tumour marker assays, five used Roche Elecsys. Read More

View Article
January 2018

ANNALS EXPRESS: HPLC methods for the analysis of endogenous cortisol and cortisone in human urine: comparison of mass spectrometry and fluorescence detection.

Ann Clin Biochem 2018 Jan 1:4563218783789. Epub 2018 Jan 1.

Uniwersytet Medyczny imienia Karola Marcinkowskiego w Poznaniu.

Background: The analysis of steroids in biological matrices is challenging. One can apply immunoassay as well as gas and liquid chromatography with various types of detection, depending on the available equipment and the experience of the analyst. The question is how the methods are interchangeable between themselves. Read More

View Article
January 2018
2 Reads

Too much of a good thing - the over requesting of cardiac troponin.

Authors:
Paul Collinson

Ann Clin Biochem 2018 Jan 1:4563218776027. Epub 2018 Jan 1.

1 Department of Clinical Blood Sciences, St George's University Hospitals NHS Foundation Trust and St George's University of London, Cranmer Terrace, London, UK.

View Article
January 2018

Therapeutic drug monitoring of tumour necrosis factor inhibitors in the management of chronic inflammatory diseases.

Ann Clin Biochem 2018 Jan 1:4563218782286. Epub 2018 Jan 1.

1 Pharmacy Service, University Hospital of Salamanca, Salamanca, Spain.

Tumour necrosis factor inhibitor therapy has drastically changed the management of chronic inflammatory diseases. Some important drawbacks that can cause loss of response during treatment with these drugs are related to their large individual variability, the disease burden and the formation of antidrug antibodies that increase its clearance. Therapeutic drug monitoring of these drugs is not yet recommended by all scientific societies, and if so, only in patients with inflammatory symptoms. Read More

View Article
January 2018

A novel high-throughput assay for the measurement of salivary progesterone by liquid chromatography tandem mass spectrometry.

Ann Clin Biochem 2018 Jan 1:4563218780904. Epub 2018 Jan 1.

3 Biochemistry Department, Wythenshawe Hospital, Manchester University NHS Trust, Manchester Academic Health Sciences Centre, Manchester, UK.

Background Liquid chromatography tandem mass spectrometry (LC-MS/MS) enables specific and sensitive quantification of steroids with a high throughput. Saliva sampling is advantageous for multisample profiling over longer periods of time, as it is non-invasive, cheap, can be carried out at home and does not require the attendance of clinical personnel. We developed a rapid LC-MS/MS for the measurement of salivary progesterone, frequently assessed as ovulation marker in patients desiring fertility. Read More

View Article
January 2018

Phenotyping and relative quantification of vitamin D binding protein in a paediatric population by using liquid chromatography-tandem mass spectrometry.

Ann Clin Biochem 2018 Jan 1:4563218780135. Epub 2018 Jan 1.

1 Laboratory of Medicine, Department of Clinical Biochemistry, Hospital Universitario Central de Asturias, Oviedo, Spain.

Background Adequate concentrations of vitamin D are required to ensure bone health and minimize the incidence of multiple extraskeletal diseases. Although total 25-hydroxyvitamin D (25OHD) remains the recommended biomarker for assessing vitamin D status, it has been speculated that free 25OHD correlates better with clinical outcomes. The calculation of free 25OHD depends on the concentrations of vitamin D binding protein (DBP), the determination of which involves different immunoassays and has led to varying results and conclusions. Read More

View Article
January 2018

Fast screening of N-glycosylation disorders by sialotransferrin profiling with capillary zone electrophoresis.

Ann Clin Biochem 2018 Jan 1:4563218779609. Epub 2018 Jan 1.

Laboratory of Metabolic Diseases, Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Background Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focusing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. Read More

View Article
January 2018
5 Reads

Renal stones.

Ann Clin Biochem 2018 Jan 1:4563218781672. Epub 2018 Jan 1.

2 Department of Urology, Lister Hospital, Stevenage, UK.

Renal stone disease is a worldwide problem which carries significant morbidity. It frequently requires specialist urology intervention. Patients with recurrent disease and those at high risk require specialist investigations and review. Read More

View Article
January 2018

An assessment of the effect of haemoglobin variants on detection by faecal immunochemical tests.

Ann Clin Biochem 2018 Jan 1:4563218778716. Epub 2018 Jan 1.

1 NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK.

Background Faecal immunochemical tests (FIT) for haemoglobin (Hb) are being used in the investigation of colorectal cancer. These tests use antibodies raised to the globin moiety of human Hb. Where the globin structure is abnormal or reduced, it is possible that antibody binding, and thus Hb-detection may be affected. Read More

View Article
January 2018

Does the mass of sample loaded affect faecal haemoglobin concentration using the faecal immunochemical test?

Ann Clin Biochem 2018 Jan 1:4563218778701. Epub 2018 Jan 1.

1 NHS Bowel Cancer Screening Programme, Guildford, UK.

Background Quantitative faecal immunochemical tests (FIT) for haemoglobin are being used for colorectal cancer (CRC) screening for asymptomatic populations and are being indicated as a suitable test to rule out CRC in symptomatic populations. Faecal samples are typically collected by patients using a probe attached to the cap of a device which is inserted into a collection device into the preservative buffer, passing through a collar to remove excess sample: this process has potential for pre-analytical error. This study investigates whether faecal haemoglobin concentration (f-Hb) results are affected by the mass and method of sample collection. Read More

View Article
January 2018

Establishment of a simpler method for measuring HDL-microRNAs.

Ann Clin Biochem 2018 Jan 1:4563218775770. Epub 2018 Jan 1.

3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.

Background MicroRNAs are present not only in exosomes but also in high-density lipoprotein (HDL) and have the potential as biomarkers for various diseases. Various purification methods have been developed to quantify HDL-miRNAs; however, they are unsuitable for clinical applications. Therefore, we aimed to establish a simpler analytical method to quantify HDL-miRNAs for clinical applications. Read More

View Article
January 2018
1 Read

The assessment of iodine status - populations, individuals and limitations.

Ann Clin Biochem 2018 Jan 1:4563218774816. Epub 2018 Jan 1.

1 Chemical Pathology and Metabolic Medicine, Department of Laboratory Medicine, University Hospital Southampton, Southampton, UK.

Iodine deficiency is a significant global health concern, and the single greatest cause of preventable cognitive impairment. It is also a growing public health concern in the UK particularly among pregnant women. Biomarkers such as urinary iodine concentration have clear utility in epidemiological studies to investigate population-level iodine status, but determination of iodine status in individuals is much more problematic with current assays. Read More

View Article
January 2018
1 Read

Troponin, delta change and the evolution of cardiac biomarkers - back to the future (again).

Authors:
Paul Collinson

Ann Clin Biochem 2018 Jan 1:4563218774809. Epub 2018 Jan 1.

Clinical Blood Sciences1 and Cardiology2, St George's University Hospitals NHS Foundation Trust, St George's University of London, London, UK.

View Article
January 2018
1 Read

The challenge of improving the diagnostic yield from metanephrine testing in suspected phaeochromocytoma and paraganglioma.

Ann Clin Biochem 2018 Jan 1:4563218774590. Epub 2018 Jan 1.

1 Department of Clinical Biochemistry, PathWest Fiona Stanley Hospital Network, Murdoch, Western Australia, Australia.

Background Plasma-free metanephrines (PFM) or urinary fractionated metanephrines (UFM) are the preferred biochemical tests for the diagnosis of phaeochromocytoma and paraganglioma (PPGL). Borderline increased results should be followed up to either exclude or confirm diagnosis. Methods We extracted all PFM and UFM results reported by our laboratory over a six-month period from the laboratory information system. Read More

View Article
January 2018
4 Reads

That '70%' claim again ….

Authors:
Mike J Hallworth

Ann Clin Biochem 2018 Jul 30;55(4):517-518. Epub 2018 Apr 30.

Department of Clinical Biochemistry, Royal Shrewsbury Hospital, Shropshire, UK.

View Article
July 2018
2 Reads

A case of hereditary coproporphyria with posterior reversible encephalopathy and novel coproporphyrinogen oxidase gene mutation c.863T>G (p.Leu288Trp).

Ann Clin Biochem 2018 Jan 1:4563218774597. Epub 2018 Jan 1.

1 Department of General Medicine, 8493 Wellington Hospital , Wellington, New Zealand.

A 21-year-old female had recurrent presentations to the emergency department with myalgia, vomiting, abdominal pain and subsequently developed generalized seizures. She was volume depleted with a plasma sodium of 125 mmol/L (reference interval: 135-145) and she had fluctuating hypertension. Acute porphyria was suspected and confirmed with raised urine porphobilinogen/creatinine ratio of 12:4 μmol/mmoL (reference interval < 1:5) and she was treated with intravenous haem arginate. Read More

View Article
January 2018
3 Reads

Postfilter ionized calcium measurements in citrate anticoagulation for continuous renal replacement therapy: How often should Ca-membrane in blood gas analyser ABL800 be replaced?

Ann Clin Biochem 2018 Jan 1:4563218773415. Epub 2018 Jan 1.

3 Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus N, Denmark.

View Article
January 2018
6 Reads

Analytical evidence to show letters impregnated with novel psychoactive substances are a means of getting drugs to inmates within the UK prison service.

Ann Clin Biochem 2018 Jan 1:4563218767462. Epub 2018 Jan 1.

SWBH NHS Trust, Birmingham, West Midlands, UK.

Introduction Novel psychotropic substances also known as legal highs are a major concern in UK prisons, fuelling violence and putting a strain on resources for inmates requiring medical treatment for adverse effects. We provide a clinical toxicology service including routine screening for novel psychoactive substances. In 2015, we were approached by Her Majesty Prison Service search dog training team to advise on which novel psychoactive substances to target, and again in 2016 to further provide analytical support to test five letters which the dogs positively identified for novel psychotropic substances during routine searches of prison mail rooms. Read More

View Article
January 2018
5 Reads

Evaluation of sample stability for a quantitative faecal immunochemical test and comparison of two sample collection approaches.

Ann Clin Biochem 2018 Jan 1:4563218766393. Epub 2018 Jan 1.

1 Department of Clinical Biochemistry, 8749 York Teaching Hospital NHS Foundation Trust , York, UK.

Background Faecal immunochemical testing is increasingly being used to triage symptomatic patients for suspected colorectal cancer. However, there are limited data on the effect of preanalytical factors on faecal haemoglobin when measured by faecal immunochemical testing. The aim of this work was to evaluate the stability of faecal haemoglobin in faeces and to compare two methods of faecal haemoglobin sampling for faecal immunochemical testing. Read More

View Article
January 2018
5 Reads

Identification of a rare haemoglobin variant with a newly reported interference in HbA1c analysis.

Ann Clin Biochem 2018 Jul 29;55(4):516-517. Epub 2018 Mar 29.

1 Department of Medical Biochemistry and Immunology, University Hospital of Wales, Cardiff, UK.

View Article
July 2018
4 Reads

Surveillance evaluation of the standardization of assay values for serum total 25-hydroxyvitamin D concentration in Japan.

Ann Clin Biochem 2018 Jan 1:4563218765570. Epub 2018 Jan 1.

8 Faculty of Health Sciences, University of Human Arts and Sciences, Saitama, Japan.

Background To assess the vitamin D nutritional status, serum total 25-hydroxyvitamin D (25(OH)D) concentration is measured. We used six automated 25(OH)D immunoassays (AIAs) available in Japan and certified by the Vitamin D Standardization Program (VDSP) at the U.S. Read More

View Article
January 2018
5 Reads

Development of a rapid liquid chromatography tandem mass spectrometry method for the quantitation of serum dexamethasone and its clinical verification.

Ann Clin Biochem 2018 Jan 1:4563218766566. Epub 2018 Jan 1.

1 Department of Clinical Biochemistry, 159812 Wythenshawe Hospital , Manchester NHS Foundation Trust, Manchester, UK.

Background Measurement of serum dexamethasone during the overnight dexamethasone-suppression test has been recommended to reduce false-positive results when investigating Cushing's syndrome or increasingly commonly found adrenal incidentalomas. Despite this, there remains a paucity of well-validated dexamethasone methods currently available. Here, we describe the development of a rapid and sensitive liquid chromatography tandem mass spectrometry serum dexamethasone assay and verify its utility in a cohort of postmenopausal females. Read More

View Article
January 2018
3 Reads

Comparison of HbA1c and glycated albumin as a control marker for newborn complications in diabetic women in a multicentre study in Japan (Japan glycated albumin study group: study 2).

Ann Clin Biochem 2018 Jan 1:4563218763695. Epub 2018 Jan 1.

4 Aiiku Maternal and Child Health Center, Imperial Gift Foundation, Tokyo, Japan.

Background To clarify the relationship between glycated haemoglobin and glycated albumin concentrations during pregnancy with neonatal outcomes, a multicentre study was conducted by the Japanese Society of Diabetes and Pregnancy. Methods A total of 136 patients (type 1: n = 47, type 2: n = 89) who enrolled in the study were diagnosed based on the Japanese Diabetes Society diagnostic criteria for diabetes mellitus. Thresholds for glycated haemoglobin and glycated albumin were set at 5. Read More

View Article
January 2018
2 Reads

Validation of an automated ultraperformance liquid chromatography IgG N-glycan analytical method applicable to classical galactosaemia.

Ann Clin Biochem 2018 Jan 1:4563218762957. Epub 2018 Jan 1.

2 National Centre for Inherited Metabolic Diseases, The Mater Misericordiae University Hospital, Dublin, Ireland.

Background Classical galactosaemia (OMIM #230400) is a rare disorder of carbohydrate metabolism caused by deficiency of the galactose-1-phosphate uridyltransferase enzyme. The pathophysiology of the long-term complications, mainly cognitive, neurological and female fertility problems, remains poorly understood. Current clinical methods of biochemical monitoring lack precision and individualization with an identified need for improved biomarkers for this condition. Read More

View Article
January 2018
4 Reads

Comment on: Standardization of steroid tests and implications for the endocrine community.

Ann Clin Biochem 2018 May 1;55(3):408. Epub 2018 Mar 1.

Weqas, Cardiff and Vale University Health Board, Cardiff, Wales, UK.

View Article
May 2018
5 Reads

Comment on: Standardization of steroid tests and implications for the endocrine community.

Ann Clin Biochem 2018 May 1;55(3):406-407. Epub 2018 Mar 1.

Retired Private Individual, Birmingham, UK.

View Article
May 2018
3 Reads

Standardization of steroid tests and implications for the endocrine community: Author's reply.

Authors:
John W Honour

Ann Clin Biochem 2018 May 1;55(3):410. Epub 2018 Mar 1.

Institute of Women's Health, UCL, London, UK.

View Article
May 2018
3 Reads

Liquid chromatography tandem mass spectrometry: challenges in introducing published methods into the clinical laboratory.

Ann Clin Biochem 2018 May 10;55(3):404-405. Epub 2018 Feb 10.

Department of Clinical Biochemistry and Metabolic Medicine, Liverpool Clinical Laboratories, Royal Liverpool University and Broadgreen University Hospitals Trust, Liverpool, UK.

View Article
May 2018
2 Reads

A letter in response to 'Liquid chromatography tandem mass spectrometry: challenges in introducing published methods into the clinical laboratory' by Khedr et al.

Ann Clin Biochem 2018 May 10;55(3):405-406. Epub 2018 Feb 10.

Department of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester, UK.

View Article
May 2018
3 Reads

Improved technical success and radiation safety of adrenal vein sampling using rapid, semi-quantitative point-of-care cortisol measurement.

Ann Clin Biochem 2018 Jan 1:4563218760352. Epub 2018 Jan 1.

1 Department of Clinical Biochemistry, PathWest 418838 Fiona Stanley Hospital Network, Murdoch, Western Australia, Australia.

Objective Primary aldosteronism is a curable cause of hypertension which can be treated surgically or medically depending on the findings of adrenal vein sampling studies. Adrenal vein sampling studies are technically demanding with a high failure rate in many centres. The use of intraprocedural cortisol measurement could improve the success rates of adrenal vein sampling but may be impracticable due to cost and effects on procedural duration. Read More

View Article
January 2018
6 Reads
2.08 Impact Factor

Post hoc analysis of plasma amino acid profiles: towards a specific pattern in autism spectrum disorder and intellectual disability.

Ann Clin Biochem 2018 Jan 1:4563218760351. Epub 2018 Jan 1.

1 26928 CHRU de Tours , Laboratoire de Biochimie et Biologie Moléculaire, Tours, France.

Objectives Autism spectrum disorders and intellectual disability present a challenge for therapeutic and dietary management. We performed a re-analysis of plasma amino acid chromatography of children with autism spectrum disorders ( n = 22) or intellectual disability ( n = 29) to search for a metabolic signature that can distinguish individuals with these disorders from controls ( n = 30). Methods We performed univariate and multivariate analyses using different machine learning strategies, from the raw data of the amino acid chromatography. Read More

View Article
January 2018
6 Reads

The bone remodelling cycle.

Ann Clin Biochem 2018 May 4;55(3):308-327. Epub 2018 Mar 4.

2 Molecular Endocrinology Laboratory, Department of Medicine, Imperial College London, London, UK.

The bone remodelling cycle replaces old and damaged bone and is a highly regulated, lifelong process essential for preserving bone integrity and maintaining mineral homeostasis. During the bone remodelling cycle, osteoclastic resorption is tightly coupled to osteoblastic bone formation. The remodelling cycle occurs within the basic multicellular unit and comprises five co-ordinated steps; activation, resorption, reversal, formation and termination. Read More

View Article
May 2018
3 Reads

Falsely elevated plasma metanephrine in patients taking midodrine.

Ann Clin Biochem 2018 Jul 21;55(4):509-515. Epub 2018 Feb 21.

1 Department of Endocrinology, Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK.

Plasma metanephrines have become the biochemical test of choice for suspected phaeochromocytomas and paragangliomas in many institutions. We encountered two separate cases of significantly elevated plasma metanephrines in patients taking midodrine, a sympathomimetic drug used in the treatment of severe postural hypotension, in the absence of a diagnosis of phaeochromocytomas and paragangliomas. Upon stopping midodrine treatment, plasma metanephrine concentrations returned to normal in both patients. Read More

View Article
July 2018
4 Reads

Diagnostic and prognostic potential of serum miR-132/212 cluster in patients with hepatocellular carcinoma.

Ann Clin Biochem 2018 Jan 1:4563218755815. Epub 2018 Jan 1.

2 Prince of Wales Clinical School, University of New South Wales, Sydney, Australia.

Background It has been reported that both of the miR-132/212 (micro-RNA) cluster members, miR-132 and miR-212, are downregulated in hepatocellular carcinoma. Nevertheless, the expression pattern and clinical utility of serum miR-132/212 in hepatocellular carcinoma are still unknown. Methods In this study, serum concentrations of miR-132 and miR-212 were measured in 80 hepatocellular carcinoma patients, 51 controls with chronic liver diseases and 42 healthy volunteers by using quantitative real-time polymerase chain reaction. Read More

View Article
January 2018
9 Reads

Difference of seasonal variation between glycated albumin and glycated haemoglobin.

Ann Clin Biochem 2018 Jan 1:4563218755816. Epub 2018 Jan 1.

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Background Glycated albumin reflects 2-3-week glycaemic controls, and in addition to glycated haemoglobin, it has been used as a glycaemic control indicator. We presumed that glycated albumin also has seasonal variations and is related to temperature, similar to glycated haemoglobin. Methods The subjects were diabetic outpatients from April 2007 to March 2013. Read More

View Article
January 2018
3 Reads

Valid analytical performance specifications for combined analytical bias and imprecision for the use of common reference intervals.

Ann Clin Biochem 2018 Jan 1:4563217752963. Epub 2018 Jan 1.

6 Department of Research, Nordsjællands Hospital, University of Copenhagen, Hillerød, Denmark.

Background Many clinical decisions are based on comparison of patient results with reference intervals. Therefore, an estimation of the analytical performance specifications for the quality that would be required to allow sharing common reference intervals is needed. The International Federation of Clinical Chemistry (IFCC) recommended a minimum of 120 reference individuals to establish reference intervals. Read More

View Article
January 2018
4 Reads

Predictive role of tear protein expression in the early diagnosis of Sjögren's syndrome.

Ann Clin Biochem 2018 Jan 1:4563217750679. Epub 2018 Jan 1.

1 Ophthalmology Unit, DIMES, University of Bologna, Bologna, Italy.

Background The contribution of tear protein expression in patients with presumed diagnosis of Sjögren syndrome is underestimated. We aimed to evaluate the role of tear proteins in the Sjögren syndrome early diagnosis. Methods Charts from 110 patients suspected of Sjögren syndrome were analysed and the subsequent diagnosis retrieved. Read More

View Article
January 2018
3 Reads

Unwanted road to anaemia in transthyretin familial amyloid polyneuropathy may continue irrespective of tafamidis treatment.

Ann Clin Biochem 2018 Jan 1:4563218754587. Epub 2018 Jan 1.

2 Department of Neurology, Kumamoto University, Graduate School of Medical Sciences, Kumamoto, Japan.

Background This retrospective longitudinal study was performed to determine whether tafamidis treatment leads to improvements in commonly used blood data for transthyretin familial amyloid polyneuropathy (TTR-FAP). Methods Commonly used blood data (complete blood count [including a haemogram], total protein, albumin, blood urea nitrogen, creatinine, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, γ-glutamyl transpeptidase, total bilirubin [T-Bil], creatine kinase, choline esterase, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, estimated glomerular filtration rate [eGFR], serum amyloid A protein, TTR, haemoglobin A1c, free triiodothyronine [FT3] and free thyroxine [FT4]) were investigated in 33 TTR-FAP patients. These values included longitudinal data at three time points: six months before or after tafamidis treatment and one year after tafamidis treatment. Read More

View Article
January 2018
6 Reads

Evaluation of cellulose pads as a method to detect cytomegalovirus DNA in neonatal urine.

Ann Clin Biochem 2018 Jan 1:4563217750183. Epub 2018 Jan 1.

1 Department of Neonatology and Pediatric Critical Care Medicine, University Children's Hospital Bonn, Bonn, Germany.

Background Several approaches exist to screen neonates for congenital cytomegalovirus infection. We here describe a new method using cellulose pads for urine collection and its evaluation in an experimental and a clinical setting. Methods We systematically tested the effect of storage duration of the pads after exposure to cytomegalovirus-positive urine, meconium contamination and specimen handling on the cytomegalovirus load and the detection rate. Read More

View Article
January 2018
5 Reads

Assessing the cost-effectiveness of a routine versus an extensive laboratory work-up in the diagnosis of anaemia in Dutch general practice.

Ann Clin Biochem 2018 Jan 1:4563217748984. Epub 2018 Jan 1.

1 Department of Health Technology and Services Research, MIRA Institute for Biomedical Technology and Technical Medicine, 3230 University of Twente , Enschede, the Netherlands.

Background Establishing the underlying cause of anaemia in general practice is a diagnostic challenge. Currently, general practitioners individually determine which laboratory tests to request (routine work-up) in order to diagnose the underlying cause. However, an extensive work-up (consisting of 14 tests) increases the proportion of patients correctly diagnosed. Read More

View Article
January 2018
7 Reads

The effectiveness of a routine versus an extensive laboratory analysis in the diagnosis of anaemia in general practice.

Ann Clin Biochem 2018 Jan 1:4563217748680. Epub 2018 Jan 1.

1 Department of Internal Medicine, 2998 Albert Schweitzer Hospital , Dordrecht, the Netherlands.

Background We investigated the percentage of patients diagnosed with the correct underlying cause of anaemia by general practitioners when using an extensive versus a routine laboratory work-up. Methods An online survey was distributed among 836 general practitioners. The survey consisted of six cases, selected from an existing cohort of anaemia patients ( n = 3325). Read More

View Article
January 2018
6 Reads

A study of high-, middle- and low-molecular weight adiponectin in urine as a surrogate marker for early diabetic nephropathy using ultrasensitive immune complex transfer enzyme immunoassay.

Ann Clin Biochem 2018 Jan 1:4563217748681. Epub 2018 Jan 1.

2 Life Style Diseases, Institute for Health Sciences, Tokushima Bunri University, Tokushima, Japan.

Background For the early identification of patients at risk of developing diabetic nephropathy, we have developed an ultrasensitive immune complex transfer enzyme immunoassay to measure adiponectin in urine. Methods We developed immune complex transfer enzyme immunoassay for adiponectin and measured urinary adiponectin from 70 healthy subjects, 35 obese non-diabetic subjects and 20 patients with diabetes. Results The urinary adiponectin concentrations in patients with diabetes (3. Read More

View Article
January 2018
5 Reads

Assessing biomarker status of vitamin B in the laboratory: no simple solution.

Ann Clin Biochem 2018 Mar 21;55(2):188-189. Epub 2017 Dec 21.

Nutrition Innovation Centre for Food and Health, School of Biomedical Science, Ulster University, Coleraine, UK.

View Article
March 2018
6 Reads

High-sensitivity cardiac troponin concentrations at emergency department presentation in females and males with an acute cardiac outcome.

Ann Clin Biochem 2017 Jan 1:4563217743997. Epub 2017 Jan 1.

5 Division of Cardiology, and Population Health Research Institute, McMaster University, Hamilton, ON, Canada.

Background There is interest in utilizing different cut-offs per sex for high-sensitivity cardiac troponin I (hs-cTnI) but less so for high-sensitivity cardiac troponin T (hs-cTnT) for patient management in the acute setting. Our objective was to assess if differences in hs-cTn concentrations exist between males and females for an acute cardiac outcome following the presentation measurement in the emergency department. Methods An observational emergency department population with hs-cTn measurements (Roche Diagnostics and Abbott Diagnostics) at presentation with seven-day outcomes for a composite acute cardiac outcome (i. Read More

View Article
January 2017
3 Reads