3,111 results match your criteria Annals of clinical and laboratory science[Journal]


The Biology of Pulmonary Coronavirus Infection Underscores Host-Directed Therapies as an Option.

Ann Clin Lab Sci 2020 Mar;50(2):287-288

Fellow, Association of Clinical Scientists, Professor in Pathology and Laboratory Medicine, UT Health McGovern Medical School at Houston, TX, USA

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Evaluation of the Automated Indirect Immunofluorescence Test for Antinuclear Antibodies.

Ann Clin Lab Sci 2020 Mar;50(2):282-286

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

The performance of the automated indirect immunofluorescence system was compared with the manual method for detection of antinuclear antibodies (ANA) from 354 clinical serum samples. We compared the results (negative or positive), ANA patterns, and titers for the two methods. The coincidence rates for ANA positive and negative samples were 93. Read More

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Analytical Performance Evaluation of Aptima Herpes Simplex Virus 1 and 2 Assay Using Hologic Panther Instrument.

Ann Clin Lab Sci 2020 Mar;50(2):278-281

Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston, Houston, TX, USA

Objective: Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are responsible for a variety of human diseases. Therefore, rapid detection of these viruses in clinical laboratory is important. Recently Aptima Herpes Simplex 1 and 2 assay has been approved by the FDA. Read More

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Clinical Utility of HIV-1/2 Ab Immunochromatographic Assay as an Additive Test for HIV Screen-Positive Patients.

Authors:
Soo Jin Yoo

Ann Clin Lab Sci 2020 Mar;50(2):274-277

Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea

Objective: Frequent false-positive results of current HIV screening occur due to its high sensitivity. These false-positives may delay subsequent clinical decision making. In this study, we aim to determine the usefulness of additional immunochromatographic assay (ICA) in hospitals using fourth-generation HIV-1/2 Ag/Ab assays followed by western blot (WB) confirmation. Read More

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Cryptic Acute Promyelocytic Leukemia (APL) Presenting as Seizures in an Adolescent.

Ann Clin Lab Sci 2020 Mar;50(2):270-273

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.

There are approximately 800 new cases of acute promyelocytic leukemia (APL) in the United States every year. APL is rarely observed in pediatric populations, and accounts for less than 5-10% of all pediatric cases of acute myeloid leukemia (AML). APL typically presents with symptoms related to the pancytopenia such as fatigue due to anemia, bleeding and bruising secondary to thrombocytopenia, and infections attributed to a lack of functioning leukocytes. Read More

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Morphoproteomics Identifies CXCR4 in Undifferentiated Colorectal Cancer: A Case Study with Therapeutic Implications.

Ann Clin Lab Sci 2020 Mar;50(2):266-269

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.

Undifferentiated colorectal cancer (UCRC) has a well-documented CD44 cancer stem cell (CSC) component. CXCR4 (C-X-C motif receptor 4) also is a CSC marker and with its ligand, stromal-derived factor (SDF)-1 alpha has been shown to act in concert with CD44 to promote cancer cell invasion. To date, CXCR4 has not been reported in the MEDLINE (PubMed) literature with respect to UCRC. Read More

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March 2020
0.839 Impact Factor

Correlation of Fentanyl Positive Drug Screens with Other Medications in Patients from Pain, Rehabilitation and Behavioral Programs.

Ann Clin Lab Sci 2020 Mar;50(2):260-265

Precision Diagnostics, San Diego, CA, USA

Fentanyl has been associated with many drug overdose deaths; its presence in many street drugs has been postulated to be increasing. We examined 1.3 million urine drug tests from April 2016 to April 2019 for fentanyl and other drugs. Read More

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Red Blood Cell Distribution Width Is Increased in Patients with Pemphigus: A Case-Control Study.

Ann Clin Lab Sci 2020 03;50(2):253-259

Department of Dermatology, Rambam Health Care Campus, Haifa, Israel

Background: Red blood cell distribution width (RDW) has recently emerged as an inflammatory marker in several inflammatory diseases but has not been investigated in patients with pemphigus.

Objective: We aimed to examine RDW percentage in patients with pemphigus relative to control subjects and to assess the association between this biomarker and the morphological characteristics of the disease.

Methods: This case-control study included 183 pemphigus patients and 915 age- and sex-matched control subjects. Read More

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Associations of Anti-MHC Class I-related Chain A (MICA) Antibodies with Renal Allograft Outcomes.

Ann Clin Lab Sci 2020 Mar;50(2):247-252

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea

The association of anti-MHC class I-related chain A (MICA) and kidney allograft rejection has been reported. However, the associations of antibodies specific to donor MICA (dsMICA) and dsMICA levels with allograft outcome have not been elucidated. From November 2009 to June 2017, 125 sera samples from renal transplantation recipients with no HLA antibodies were screened for MICA antibodies. Read More

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Distribution of Carbapenemases and Efflux Pump in Carbapenem-resistance .

Ann Clin Lab Sci 2020 Mar;50(2):241-246

Department of Clinical Laboratory, Lianshui County People's Hospital, Lianshui, China

has emerged as an important pathogen related to serious infections and nosocomial outbreaks around the world. In this study, 100 isolates of carbapenem-resistance in (CRAB) were collected from clinical specimens. Agar dilution was conducted to determine the minimum inhibitory concentrations (MICs) for 15 kinds of antibiotics. Read More

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The Role of Serum Calcium in Assessing Metabolic Syndrome in Colorectal Cancer.

Ann Clin Lab Sci 2020 Mar;50(2):233-240

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

Objective: The association of serum calcium with metabolic syndrome in colorectal cancer (CRC) is largely unknown. This study investigated the relationship between serum calcium and patients' clinical characteristics, as well as metabolic parameters in CRC patients.

Methods: CRC patients in Peking University People's Hospital from June 2015 to December 2018 were included; serum calcium, HDL (high-density lipoprotein), LDL (low-density lipoprotein), TC (total cholesterol), TG (triglyceride), FPG (fasting plasma glucose) and albumin were detected. Read More

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Efficacy of Serum Total Bilirubin in Predicting the Severity of Ulcerative Colitis: A Cross-Sectional Study.

Ann Clin Lab Sci 2020 Mar;50(2):228-232

Department of Gastroenterology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu Province, China

Objective: Several serum parameters can be used to assess ulcerative colitis (UC) disease activity. However, the value of these parameters for predicting UC severity has not been studied in depth. Therefore, we sought to investigate the value of serum total bilirubin (TB) as a predictor of UC severity. Read More

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Circulating miRNA-199a and miRNA-122 Levels as Potential Diagnostic and Prognostic Biomarkers for Hepatocellular Carcinoma.

Ann Clin Lab Sci 2020 Mar;50(2):219-227

Department of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Fujian, China

Objective: MicroRNAs (miRNAs) are associated with hepatocellular carcinoma (HCC) progression and metastasis. However, it is unclear whether they could act as biomarkers for HCC diagnosis and prognosis.

Methods: In this study, the miR-122 and miR-199a expression levels were determined by quantitative real-time PCR (qRT-PCR). Read More

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Plasmablastic Lymphomas: Characterization of Tumor Microenvironment Using CD163 and PD-1 Immunohistochemistry.

Ann Clin Lab Sci 2020 Mar;50(2):213-218

Department of Pathology, UT Southwestern Medical Center, Dallas, TX, USA

This study aims to characterize the tumor microenvironment of plasmablastic lymphoma (PBL) in regard to the quantities of CD163(+) tumor associated macrophages (TAM) and PD1(+) tumor infiltrating lymphocytes (TIL). This article also reviews the existing knowledge of the role of PD-1/PD-L1 pathway in the tumor microenvironment of hematopoietic neoplasms, discusses potential mechanisms to explain our findings, and outlines areas for future studies. We performed CD163 and PD1 immunohistochemical studies in 11 cases classified as plasmablastic lymphoma, and recorded the percentages of positive TAMs and TILs. Read More

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Taxotere-induced WNT16 Expression in Carcinoma-Associated Fibroblasts Might Associate with Progression and Chemoresistance of Breast Cancer.

Ann Clin Lab Sci 2020 Mar;50(2):205-212

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China

Objective: The objective of this study is to investigate the relationship between WNT16 expression in carcinoma-associated fibroblasts and Taxotere treatments in breast cancer.

Procedures: WNT16 expression in primarily cultured carcinoma-associated fibroblasts was detected via Real-Time PCR and Western blot. A total of 106 breast cancer cases were prepared for immunohistochemistry. Read More

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Ki-67 and Clinical Correlations in Patients with Resistant Prolactinomas.

Ann Clin Lab Sci 2020 Mar;50(2):199-204

Department of Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Objective: Drug resistance is the main limitation for dopamine agonist therapy with prolactinoma. Transsphenoidal surgery is an additional operation that circumvents this limitation. To characterize distinctive clinical features of resistant prolactinoma in different levels of Ki-67, a retrospective analysis of 423 patients who were diagnosed and surgically treated at Shandong Provincial Hospital Affiliated to Shandong University from January 2007 to June 2017 was performed. Read More

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Effects of Oxysterols on Chondrogenesis of Human Adipose-derived Stem Cells.

Ann Clin Lab Sci 2020 Mar;50(2):190-198

Korean Medical Research Center for Healthy Aging and School of Korean Medicine, Busan, Korea

Human adipose-derived stem cells (hADSCs) have been implicated as a high potency source of chondrocytes in cell therapy for cartilage defects. However, appropriate stimulators for the chondrogenesis of hADSCs are needed. Oxysterols have the potential to act as a stimulator. Read More

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MiR-1247-5p Functions as a Tumor Suppressor in Human Astroglioma Cells by Targeting .

Ann Clin Lab Sci 2020 Mar;50(2):182-189

Department of Neurosurgery, The Second Clinical Medical College, Yangtze University, China

Malignant glioma is one of the most common primary tumors of the central nervous system. MicroRNAs (miRNAs) play an important role in the occurrence of gliomas, and have a potential value in their treatment. In this study, we investigated the effect of miR-1247-5p on glioma progression. Read More

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Computer-assisted Diagnosis of Breast Cancer by Cell Network Matrix Extraction and Multilayer Perceptron Analysis.

Ann Clin Lab Sci 2020 Mar;50(2):175-181

Department of Pathology, Kings County Hospital Center, Brooklyn, New York, USA

Objective: Diagnosis of breast cancer is based on identification of various morphologic features by histopathologic examination of the specimen. Ancillary immunohistochemical and molecular analyses provide additional information that is prognostic and guides therapy. Because of subjectivity in this approach, we sought to develop a computer model which could assist in differentiating normal or benign tissue from malignant breast carcinoma. Read More

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Anti-TNF-α Biologics Do Not Reverse CDX2 Downregulation in Patients with Crohn's Disease.

Ann Clin Lab Sci 2020 Mar;50(2):172-174

Department of Medicine, Section of Gastroenterology, Hepatology and Nutrition and the Ertan Digestive Diseases Center of Excellence, The University of Texas Health Science Center at Houston McGovern Medical School, and Memorial-Hermann Hospital-TMC, Houston, TX, USA.

Objective: The caudal-related homeobox transcription factor 2 (CDX2) plays an important role in intestinal epithelial differentiation, proliferation, migration, and adhesion. It has been previously reported that TNF-α reduces CDX2 expression in cultured colon epithelial cells in a dose-dependent manner, and that this effect was reduced by adding the anti-TNF-α drug infliximab to the culture medium. The aim of this study was to determine whether CDX2 expression is reduced in biopsies from patients with Crohn's disease (CD), and whether treatment with anti-TNF-α drugs reverses CDX2 downregulation in these patients. Read More

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C-reactive Protein Is a Predictor of Prognosis of Prostate Cancer: A Systematic Review and Meta-Analysis.

Ann Clin Lab Sci 2020 Mar;50(2):161-171

Department of Urology, West China Hospital, West China Medical School, Sichuan University, Chengdu, China

Increasing evidence suggested that inflammation is associated with the pathogenesis and progression of several solid tumors. This study was conducted to explore the association between C-reactive protein (CRP) and survival of prostate cancer (PCa). An electronic search was completed on the basis of PubMed, Embase and Cochrane Central Register of Controlled Trials. Read More

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Herbert Derman, MD (1921-2019).

Ann Clin Lab Sci 2020 Mar;50(2):159-160

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Distortions but not Definitive Peaks by Capillary Serum Protein Electrophoresis Indicate Monoclonal Gammopathy of Undetermined Significance rather than Multiple Myeloma.

Ann Clin Lab Sci 2020 Jan;50(1):151-152

Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School, Houston, TX, USA

Objective: Capillary electrophoresis of serum proteins demonstrates occasional distortions. Distortions or peaks in the gamma, beta, and alpha-2 zones may represent monoclonal gammopathy. In this study, we investigated if such distortions are associated with monoclonal gammopathy of undetermined significance (MGUS) or multiple myeloma. Read More

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January 2020

Myelodysplastic Syndrome with Complex Chromosomal Abnormalities: A Case Report and Literature Review.

Ann Clin Lab Sci 2020 Jan;50(1):146-150

Department of Hematology, The First Hospital of Shanxi Medical University, Taiyuan, Chian, China

Objective: Karyotype is the most important diagnostic and prognostic parameter in myelodys-plastic syndrome (MDS). Here, we describe a novel case of MDS with complex chromosomal abnormalities.

Case Presentation: A 55-year-old Chinese female was admitted to the hospital for facial edema and a loss of appetite. Read More

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January 2020

First Korean Case of Coffin-Siris Syndrome with a Novel Frameshift Mutation.

Ann Clin Lab Sci 2020 Jan;50(1):140-145

Department of Pediatrics, Busan Paik Hospital, Inje University College of Medicine, Busan, Korea

Coffin-Siris Syndrome (CSS) is a rare neurodevelopmental disorder characterized by intellectual disability, coarse facial features, hypoplastic digits/nails, and hypertrichosis. The genes causative of CSS mainly encode the SWI/SNF complex, which contributes to chromatin remodeling and regulates the access of transcriptional factors to specific gene sites. While mutations account for a third of all CSS cases, the condition's phenotypic features vary widely. Read More

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January 2020

A Rare Episode of Extensive Bone Marrow Necrosis in a Chemotherapy-naïve Patient with Multiple Myeloma Exhibiting and Mutations.

Ann Clin Lab Sci 2020 Jan;50(1):136-139

Department of Laboratory Medicine, Hallym University Sacred Heart Hospital, Anyang, Korea.

Bone marrow necrosis (BMN) is a rare life-threatening condition in which the marrow is replaced by necrotic material. Half of BMN occurrences are attributed to chemotherapy or granulocyte-colony stimulating factor treatment in patients with hematolymphoid malignancies. However, we present a patient diagnosed with both multiple myeloma and extensive BMN despite being treatment-naïve. Read More

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January 2020

Infection in a Child with Phenylketonuria.

Ann Clin Lab Sci 2020 Jan;50(1):134-135

Department of Pediatric Gastroenterology, University of Alberta, Stollery Children's Hospital, Edmonton, AB, Canada.

Disabled individuals may be at risk for common and rare infections. We report on a 13-year-old female who had a diagnosis of phenylketonuria (PKU). The child received a percutaneous endoscopic gastrostomy (PEG) feeding tube at five years of age for the supplementation of her specialized formula. Read More

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January 2020

Usefulness of Plasma Glucose Adjusted #C (labile HbA1c fraction) Levels for the Screening of Variant Hemoglobin with Diabetes Mellitus.

Ann Clin Lab Sci 2020 Jan;50(1):128-133

Department of Clinical Laboratory Medicine, Hyogo College of Medicine, Hyogo, Japan.

Labile HbA1c migrates in the #C fraction with modified hemoglobin (Hb) (such as carbamylated Hb, acetaldehyde Hb, and acetylated Hb) when HbA1c is measured by Arkray's high-performance liquid chromatography (HPLC). We previously reported the usefulness of #C levels for the screening of variant Hb without diabetes mellitus. Because the #C levels are affected by plasma glucose levels, we investigated the usefulness of plasma glucose adjusted #C (PGa#C) for the screening of variant Hb complicated with diabetes mellitus. Read More

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January 2020

Comparison of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS) and the Vitek 2 System for Routine Identification of Clinically Relevant Bacteria and Yeast.

Ann Clin Lab Sci 2020 Jan;50(1):119-127

Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital Civil de Guadalajara "Fray Antonio Alcalde" e Instituto de Patología Infecciosa y Experimental, Guadalajara, Mexico

Objective: In this study, we compared the observed agreement and correlation of the Vitek 2 system with the biomarker-based MALDI-TOF MS identification results of bacteria and yeast on a routine basis.

Methods: Clinical isolates collected from two years were included. Isolates were identified using the Vitek 2 system and MALDI-TOF MS. Read More

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January 2020

GeneReader NGS System Is a Useful Sequencing Platform for Clinical Testing of and .

Ann Clin Lab Sci 2020 Jan;50(1):107-118

Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.

Although next-generation sequencing (NGS) is widely used for sequencing analysis, it involves a fragmented workflow along with complex bioinformatic analysis and interpretation. In this study, the performance characteristics and workflow of the GeneReader NGS System (QIAGEN), including sequencing, were evaluated. For genetic testing, we conducted library preparation, emulsion PCR, and sequencing. Read More

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January 2020

Dynamic Variations in Gut Microbiota in Ankylosing Spondylitis Patients Treated with Anti-TNF-α for Six Months.

Ann Clin Lab Sci 2020 Jan;50(1):99-106

Department of Gastroenterology/Endoscopy Center, Changchun, Jilin, China

Ankylosing spondylitis (AS) is known as a microbiome-driven disease; however, the current understanding of microbiota dynamics in AS is limited. In the present study, we conducted a 16S rDNA sequence-based microbiota survey of 97 fecal samples from healthy subjects and AS patients at baseline, 1, 3 and 6 months after anti-TNF-α treatment to demonstrate the dynamic characteristic variations of gut microbiota in AS patients. The goal of this experiment is to explore the values of gut microbiota as biomarkers of disease activity and therapeutic responses to anti-TNF-α. Read More

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January 2020

Designed Artificial Transcription Factors Inhibit Hepatitis B Virus Transcription in HepG2.2.15 Cells.

Ann Clin Lab Sci 2020 Jan;50(1):92-98

Department of Oncology, the People's Hospital of Chongqing Hechuan, Hechuan District, China

Hepatitis B virus (HBV) causes serious health issues worldwide. Despite this, current treatment options for HBV have many drawbacks. Strategies to safely and specifically target HBV replication and survival at the transcriptional level within host cells are needed to combat current drawbacks in treatment. Read More

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January 2020
0.839 Impact Factor

Effects of Cyclopamine on the Viability of Articular Chondrocytes in Rats with Adjuvant Arthritis .

Ann Clin Lab Sci 2020 Jan;50(1):85-91

Microscopic Morphological Center Laboratory, Anhui Medical University, China

The aim of the present study is to investigate the effect of cyclopamine, a hedgehog signaling pathway inhibitor, on adjuvant arthritis (AA), rat articular chondrocyte viability, and part mechanisms In this study, an AA rat model was established by Freund's complete adjuvant (FCA). The arthritis index (AI), secondary paw swelling degree, and HE staining were used to evaluate whether the model was successfully established. Chondrocytes of the ankle joint of AA rats were cultured and identified. Read More

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January 2020

Relationship of Polymorphism of Adhesion Molecules VCAM-1 and ICAM-1 with Preeclampsia.

Ann Clin Lab Sci 2020 Jan;50(1):79-84

Department of Obstetrics and Gynecology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.

Objective: To investigate the relationship of polymorphism in vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) with preeclampsia (PE).

Methods: The present study included 252 patients with PE and 200 healthy pregnant women as control admitted to our hospital from February 2012 to December 2016. Allelic discrimination of the rs5498 polymorphisms from the ICAM-1 gene and rs3181092 from the VCAM-1 gene was assessed using the TaqMan assay. Read More

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January 2020

Clinical Application of Folate Receptor-Mediated Staining Solution Detection in Cervical Cancer Screening.

Ann Clin Lab Sci 2020 Jan;50(1):73-78

Obstetrics and Gynecology Department, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, China.

Objective: Cervical cancer is the fourth most deadly women's cancer worldwide, and regular screening is essential to lower mortality rates. The folate receptor-mediated staining solution detection (FRD) has been suggested to be a rapid and cost-effective screening method. We aim to evaluate the validity of FRD testing in cervical cancer screening. Read More

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January 2020

Melatonin Inhibits TE-1 Esophageal Cancer Cells Metastasis by Suppressing the NF-κB Signaling Pathway and Decreasing MMP-9.

Ann Clin Lab Sci 2020 Jan;50(1):65-72

Department of Pharmacy, Affiliated Cancer Hospital of Nantong University, Nantong, Jiangsu, China

Melatonin is an amine hormone produced by mammals and the human pineal gland. Modern biomedical research has shown that melatonin has antitumor effects. However, the underlying mechanisms of these effects remain unclear. Read More

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January 2020
0.839 Impact Factor

Neuroprotective Effects of Umbilical Cord-Derived Mesenchymal Stem Cells on Radiation-Induced Brain Injury in Mice.

Ann Clin Lab Sci 2020 Jan;50(1):57-64

Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Weihui, China

Objective: To investigate the neuroprotective effects of umbilical cord-derived mesenchymal stem cells (UC-MSCs) on radiation-induced brain injury (RIBI).

Methods: Thirty female C57BL/6 mice were randomly divided into three groups: control (CON), whole brain irradiation (WBI), and the cell therapy (MSC) group. Mice in the WBI and MSC groups received a single, whole brain irradiation treatment with 15 Gy of Co. Read More

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January 2020

EZH2 Downregulation Augments the Effect of Irradiation in Reducing Pancreatic Cancer Cell Proliferation .

Ann Clin Lab Sci 2020 Jan;50(1):45-56

Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn, New York, NY, USA

Objective: Pancreatic ductal carcinoma has a 5-year survival rate of 9%. This makes it the 4th leading cause of cancer-related death in the United States. Advanced-stage diagnosis and limited treatment options contribute to poor prognosis. Read More

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January 2020

Yeast Extract Induces Apoptosis and Cell Cycle Arrest via Activating p38 Signal Pathway in Colorectal Cancer Cells.

Ann Clin Lab Sci 2020 Jan;50(1):31-44

Department of Anatomy, Jeju National University, Jeju-Do, Korea

The microbiome has recently become a key interest for cancer research. Anti-tumor effects of reinforced clostridium media (RCM) were investigated for all ingredients of RCM, which showed that yeast extract could be a candidate for this phenomenon. MTT assay, cell counting, cell death analysis, cell cycle analysis, and Western blotting were done on colorectal cancer cells with or without 5-fluorouracil resistance (SNU-C5 and SNU-C5/5-FUR). Read More

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January 2020
0.839 Impact Factor

CAPN6 Regulates Uterine Leiomyoma Cell Proliferation and Apoptosis through the Rac1-Dependent Signaling Pathway.

Ann Clin Lab Sci 2020 Jan;50(1):24-30

Department of Obstetrics and Gynecology, Affiliated Jiangyin Hospital of South-East University, Jiangyin, China

Objective: Calpain 6 (CAPN6) is one of the calcium-dependent intracellular nonlysosomal proteases that are dysregulated in uterine leiomyomas (UtLMs). However, its function and mechanism in UtLMs is still unknown.

Methods: The correlation between CAPN6 expression and UtLMs was analyzed by the Gene Expression Omnibus (GEO). Read More

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January 2020

FZD8 Indicates a Poor Prognosis and Promotes Gastric Cancer Invasion and Metastasis via B-Catenin Signaling Pathway.

Ann Clin Lab Sci 2020 Jan;50(1):13-23

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China

FZD8, a G protein-coupled receptor protein belonging to the Frizzled family, is considered to play an important role in cancer invasion and metastasis. However, the function of FZD8 in the invasion and metastasis of gastric cancer (GC) has not been elucidated. In this study, we first confirm that FZD8 protein expression was significantly upregulated in gastric cancer tissue and has a potential to be an independent predictor of poor prognosis for patients with GC. Read More

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January 2020

The Effects of Follistatin on the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Neurons-Like Cells.

Ann Clin Lab Sci 2020 Jan;50(1):3-12

Department of Liaoning Provincial Key Laboratory of Follicle Development and Reproductive Health, the Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.

Objective: The present study was designed to evaluate the effects of Follistatin (FST) on the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into neuron-like cells.

Materials And Methods: hBMSCs were isolated and characterized by cell surface markers including CD29, CD44, CD166, CD34, CD14, and CD45. Subsequently, 0. Read More

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January 2020
0.839 Impact Factor

Whole Exome Sequencing Identified a 13 Base Pair Deletion-mutation in a Patient with Restrictive Cardiomyopathy and Left Ventricle Hypertrophy.

Ann Clin Lab Sci 2019 Nov;49(6):838-840

The Second Xiangya Hospital of Central South University, Changsha, China

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November 2019

Indications for RBC Exchange Transfusion in Patients with Sickle Cell Disease: Revisited.

Authors:
Samir K Ballas

Ann Clin Lab Sci 2019 Nov;49(6):836-837

Cardeza Foundation for Hematologic Research, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA

Clinically, sickle cell disease (SCD) is characterized by chronic hemolytic anemia, recurrent acute vaso-occlusive crises, and progressive multi-organ failure. The management for SCD varies greatly among patients, and in the same patient longitudinally. Some patients may benefit from minimal therapy and infrequent transfusions, while others are transfusion dependent. Read More

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November 2019

The Intent and Process of Immunohistochemistry Scoring Algorithm Development in a Predictive Medicine Era.

Ann Clin Lab Sci 2019 Nov;49(6):835

Department of Companion Diagnostic Pathology, Roche Molecular Solutions, Tucson, AZ, USA.

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November 2019

Continuous Hemofiltration Plus Plasma Exchange in Patient with Kawasaki Disease Shock Syndrome: A Case Report and Literature Review.

Ann Clin Lab Sci 2019 Nov;49(6):829-834

Department of Critical Care Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China

A 3-year-old girl was diagnosed with Kawasaki disease shock syndrome (KDSS) and presented with multiple organ dysfunction. She was treated with bundle therapy from IVIG, aspirin, methylprednisolone, and continuous hemofiltration for fluid overload and acid-base imbalance. This process was followed by plasma exchange therapy twice. Read More

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November 2019

Hematolymphoid Malignancies Presenting with Spinal Epidural Mass and Spinal Cord Compression: A Case Series with Rare Entities.

Ann Clin Lab Sci 2019 Nov;49(6):818-828

Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR, USA

The epidural space is an uncommon site for involvement by hematolymphoid malignancies, and may present unexpectedly with neurological symptoms related to spinal cord compression. Our objective was to review the clinical and pathologic features of cases with initial presentations of cord compression, subsequently diagnosed as a hematolymphoid malignancy after pathologic examination. Review of the Department of Pathology's archives revealed 15 patients who presented with spinal cord compression due to epidural hematolymphoid malignancies between 2008-2019. Read More

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November 2019

Immunoexpression of 5-methylcytosine (5mc) in Bone Marrow Haematopoietic Cells in Patients with Myelodysplastic Syndromes.

Ann Clin Lab Sci 2019 Nov;49(6):810-817

Division of Haematology, Clinical Department of Internal Medicine, Clinical Hospital "Sveti Duh", Zagreb, Croatia.

Myelodysplastic syndromes (MDS) are biologically and clinically heterogenous groups of clonal haematopoietic stem cell diseases characterized by ineffective haematopoiesis and peripheral blood cytopenia, with a variable tendency to transform within acute leukaemia (AL). DNA hypermethylation and hypo-methylation are associated with cancer. Thus, the hypermethylation of DNA is essential for the molecular pathophysiology of MDS by inactivating genes involved in cell growth, differentiation and apoptosis. Read More

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November 2019

Determination of Normal HbA1C Levels in Non-Diabetic Patients with Hemoglobin E.

Ann Clin Lab Sci 2019 Nov;49(6):804-809

Division of Hematology, Department of Internal Medicine, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand.

Objective: Hemoglobin E (Hb E) possesses an influence on HbA1c levels. Careful interpretation of HbA1c is needed in areas with a high prevalence of Hb E, including Thailand. Therefore, the normal levels of HbA1c in non-diabetic Hb E subjects were collected and assessed. Read More

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November 2019

Knockdown of PDRG1 Could Inhibit the Wnt Signaling Pathway in Esophageal Cancer Cells.

Ann Clin Lab Sci 2019 Nov;49(6):794-803

Department of Thoracic Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui, China

Objective: PDRG1, a short p53 and DNA damage-regulated gene, is overexpressed in many human tumors. This gene can promote proliferation and inhibit apoptosis in lung cancer. The aim of this study is to evaluate the effects of the PDRG1 gene on the Wnt signaling pathway in esophageal cancer cells. Read More

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November 2019