2,968 results match your criteria Annals of clinical and laboratory science[Journal]


The 139th Meeting of the Association of Clinical Scientists, May 15-18, 2019, Hershey, PA.

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Ann Clin Lab Sci 2019 Jan;49(1):151

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January 2019

The Association between Platelet Distribution Width and Stroke in Atrial Fibrillation Patients.

Ann Clin Lab Sci 2019 Jan;49(1):143-147

Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Hypertension Research Laboratory, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China

Aims: To explore the association between platelet distribution width (PDW) and stroke in atrial fibrillation (AF) patients.

Methods: This study included 150 AF patients who were divided into stroke and non-stroke groups. Multivariate logistic regression analysis and area under the receiver operating characteristic curve were performed. Read More

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January 2019

Vitamin D and Urinary Tract Infection: A Systematic Review and Meta-Analysis.

Ann Clin Lab Sci 2019 Jan;49(1):134-142

The Second Department of Urology, Anhui Provincial Children's Hospital, Hefei, China

Whether or not Vitamin D deficiency is associated with urinary tract infections (UTI) remains controversial. We retrieved relevant articles from the PubMed, Google Scholar, the Cochrane Library, Med-line and Embase databases up to Mach 1, 2018 for studies investigating the association between Vitamin D and UTI. The meta-analysis of 9 studies included 1921 participants, of which 580 were diagnosed with UTI. Read More

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January 2019

Automated Versus Conventional Microscopic Interpretation of Antinuclear Antibody Indirect Immunofluorescence Test.

Ann Clin Lab Sci 2019 Jan;49(1):127-133

Department of Laboratory Medicine, Chungnam National University Hospital, Daejeon, Republic of Korea

Background: The detection of antinuclear antibodies (ANAs) is crucial for the diagnosis of systemic autoimmune rheumatic diseases. Here, we evaluated the performance of an automated ANA image analyzer, EUROPattern Suite.

Methods: We compared the automated and manually revised interpretations of EUROPattern Suite with conventional microscopic interpretations of indirect immunofluorescence (IIF) by calculating the analytical sensitivity and specificity for ANA detection and evaluating the concordance between these approaches for ANA detection, pattern recognition, and titer determination. Read More

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January 2019

Proteomics Analysis of FLT3-ITD Mutation in Acute Myeloid Leukemia Using Deep Learning Neural Network.

Ann Clin Lab Sci 2019 Jan;49(1):119-126

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center McGovern Medical School, Houston, TX, USA

Deep Learning can significantly benefit cancer proteomics and genomics. In this study, we attempted to determine a set of critical proteins that were associated with the FLT3-ITD mutation in newly-diagnosed acute myeloid leukemia patients. A Deep Learning network consisting of autoencoders formed a hierarchical model from which high-level features were extracted without labeled training data. Read More

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January 2019
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Significant Association of Promoter Hypomethylation with Stroke in a Chinese Population with Primary Hypertension.

Ann Clin Lab Sci 2019 Jan;49(1):112-118

College of Traditional Chinese Medicine of Jinan University, the First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China

Objective: Hypertension and hyperhomocysteinemia are both stroke risk factors. plays a vital role in one-carbon metabolism via encoding a protein with three distinct enzymatic activities (methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase, and formate-tetrahydrofolate ligase). We aimed to elucidate the association between promoter methylation and stroke in a Chinese population with primary hypertension. Read More

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January 2019
3 Reads

Simvastatin and Simvastatin-Ezetimibe Improve the Neurological Function and Attenuate the Endothelial Inflammatory Response after Spinal Cord Injury in Rat.

Ann Clin Lab Sci 2019 Jan;49(1):105-111

Department of Neurosurgery, E-Da Cancer Hospital, I-Shou University, Taiwan

During a spinal cord injury (SCI), mechanical trauma rapidly leads to a blood-spinal cord barrier (BSB) disruption, neural cell damage, axonal damage, and demyelination, followed by a cascade of secondary inflammatory reactions. These inflammatory responses spread the damage to the neural cells and impair the recovery of neurological functions. In the present study, we evaluated the efficacy of simvastatin and a simvastatin-ezetimibe combination therapy in managing the endothelial inflammatory response in an SCI rat model. Read More

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January 2019
7 Reads

Dioscin Attenuates Myocardial Damages in Diabetic Rats maybe by Regulating NO-sGC-cGMP-PKG Pathway.

Ann Clin Lab Sci 2019 Jan;49(1):97-104

Department of Emergency, Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China

Purpose: The objective of this research was to explore the effect of dioscin on myocardium in streptozotocin (STZ)-induced diabetic rats and the underlying mechanisms.

Methods: Diabetic rat model was established by a single intravenous injection of streptozocin (STZ). The rats were divided into 5 groups: control group, control+dioscin group, model group (diabetes), DDL group (diabetic rats treated with 100 μg/kg/day dioscin) and DDH group (diabetic rats treated with 200 μg/kg/day dioscin). Read More

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January 2019

Fatal Intracranial Hemorrhage in a Young Pregnant Patient with Acute Promyelocytic Leukemia.

Ann Clin Lab Sci 2019 Jan;49(1):94-96

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.

Acute promyelocytic leukemia during pregnancy is associated with a high risk of fetal and obstetrical complications. Although the prognosis of acute promyelocytic leukemia is favorable due to disease-specific drugs, such as all-trans retinoic acid, early death due to fatal intracranial hemorrhage has been observed in some cases. In this study, we present a case of catastrophic intracranial hemorrhage in a young pregnant patient with the finding of leukemic involvement of the placenta. Read More

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January 2019

Role of Red Blood Cell Distribution Width in Predicting Cardiac Syndrome X in Women.

Ann Clin Lab Sci 2019 Jan;49(1):89-93

Department of Cardiology, Tianjin Nankai Hospital, Tianjin, People's Republic of China.

Objective: To investigate whether the increased red blood cell distribution width (RDW) is a biomarker for predicting the presence of cardiac syndrome X (CSX) in women.

Methods: 110 CSX women patients and 98 normal controls were prospectively enrolled in this study. The CSX group consisted of patients with anginal chest pain, ischemia on noninvasive stress test and a normal coronary angiography. Read More

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January 2019

Complement Component 3 as a Surrogate Hallmark for Metabolic Abnormalities in Patients with Chronic Hepatitis C.

Ann Clin Lab Sci 2019 Jan;49(1):79-88

Department of Gastroenterology and Neurology, Kagawa University School of Medicine, Kagawa, Japan

Objective: We investigated the correlation between serum complement component 3 (C3) levels and metabolic and histological abnormalities in patients with chronic hepatitis C (CH-C).

Methods: Obesity and insulin resistance were estimated by calculating body mass index (BMI) and the values of the homeostasis model for assessment of insulin resistance (HOMA-IR), respectively. Severity of hepatic steatosis and fibrosis were evaluated by New Inuyama Classification and the classification proposed by Brunt and colleagues, respectively. Read More

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January 2019
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Influence of Docosahexaenoic Acid on Proliferation and Apoptosis in Human HepG2 Cell Line.

Ann Clin Lab Sci 2019 Jan;49(1):72-78

Department of General Surgery, Ningbo NO. 2 Hospital, Ningbo, China.

Objective: This study aims to investigate the effect of different concentrations of docosahexaenoic acid (DHA) on proliferation and apoptosis in HepG2 cell lines, and to research the possible molecular mechanisms.

Methods: DHA concentration was 0 g/mL in the negative control group, and 15, 30, 45, 60 and 75 ug/mL, respectively, in the experimental groups. CCK-8 and flow cytometry methods were used to observe the growth inhibition and apoptosis rates of HepG2 cells cultured , which were treated with different concentrations of DHA. Read More

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January 2019

Dioscin Attenuates Myocardial Damages in Diabetic Rats maybe by Regulating NO-sGC-cGMP-PKG Pathway.

Ann Clin Lab Sci 2019 Jan;49(1):63-104

Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Chinese Ministry of Public Health and Key Laboratory of Medical Cell Biology, Chinese Ministry of Education, China Medical University, China

This study aimed to evaluate the use of Insulin-Transferrin-Selenium (ITS) medium in place of fetal bovine serum (FBS) to culture human amnion mesenchymal stem cells (hAMSCs). Cell morphology, ultrastructure, proliferation, migration and MSC related markers were assessed accordingly. The hAMSCs were induced to osteocyte, chondrocyte, adipocyte and keratinocyte by culturing in appropriate induction medium. Read More

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January 2019
3 Reads

Serum 14-3-3η Could Improve the Diagnostic Rate of Rheumatoid Arthritis and Correlates to Disease Activity.

Ann Clin Lab Sci 2019 Jan;49(1):57-62

School of Medical Laboratory, Tianjin Medical University, Tianjin, China

Objectives: Serum 14-3-3η is a novel joint-derived proinflammatory mediator associated with rheumatoid arthritis (RA). This study aimed to evaluate the diagnostic capacity of serum 14-3-3η and its correlation with clinical variables in patients with RA.

Methods: A total of 94 patients with RA and 80 age- and sex-matched controls, including 40 healthy subjects, were included. Read More

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January 2019
1 Read
0.839 Impact Factor

Epitope Analysis Aids in Transplant Decision Making by Determining the Clinical Relevance of Apparent Pre-Transplant Donor Specific Antibodies (DSA).

Ann Clin Lab Sci 2019 Jan;49(1):50-56

Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

Pre-transplantation work-up on a patient with end stage renal disease using Single Antigen Bead (SAB) testing showed significant anti-HLA-B*44:02 (>5,000 MFI) and anti-HLA-B*44:03 (>1,000 MFI) antibodies, with persistence on quarterly testing. No significant Class II anti-HLA antibodies were present. The patient received a potential offer from a living unrelated-donor expressing HLA-B*44:02. Read More

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January 2019
4 Reads

Reproductive Systemic Toxicity and Mechanism of Glucosides of Hook. F. (GTW).

Ann Clin Lab Sci 2019 Jan;49(1):36-49

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China

Background: To study the mechanism of reproductive systemic toxicity of on SD rats.

Methods: The SD rats were randomly divided into groups and allocated three different treatments: control, 37.8 mg/kg Tripterygium glycosides, and 94. Read More

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January 2019
4 Reads

Dexmedetomidine Attenuates Renal and Myocardial Ischemia/Reperfusion Injury in a Dose-Dependent Manner by Inhibiting Inflammatory Response.

Ann Clin Lab Sci 2019 Jan;49(1):31-35

Department of Critical Care Medicine, Mianyang Central Hospital, Mianyang, China.

Objective: To investigate the effect of dexmedetomidine (DEX) against renal and myocardial ischemia/reperfusion (I/R) injury induced by renal I/R and explore its potential mechanism.

Methods: Male Wistar rats were randomly allocated to sham, I/R, D1, D2 and D3 group. The sham group received laparotomy without a renal ischemia. Read More

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January 2019
3 Reads

The Effect of Triggering Receptor Expressed by Myeloid Cells 2 Modified Bone Marrow Mesenchymal Stem Cells on Alzheimer's Disease-Mouse Model.

Ann Clin Lab Sci 2019 Jan;49(1):23-30

Department of Neurology, Jiangxi Provincial People's Hospital, Affiliated to Nanchang University, Nanchang, China

Objective: This study aims to explore the effect of TREM2 modified BMSCs on hippocampus of AD mice.

Methods: Mouse bone marrow mesenchymal stem cells were isolated and identified. APP/PS1 double transgenic mice were confirmed to be AD model and divided into 4 groups: control group, MSCs group, MSCs+vector group and MSCs+pEGFP-TREM2 group. Read More

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January 2019

Effects of the Modified Bazhen Decoction in the Treatment of Premature Ovarian Failure in Rats.

Ann Clin Lab Sci 2019 Jan;49(1):16-22

Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objective: This study aims to investigate the role of X-linked inhibitor of apoptosis protein (XIAP) in the pathogenesis of premature ovarian failure (POF) and the effects of the Modified Bazhen Decoction (MBD) in the treatment of POF.

Materials And Methods: Twenty-four eight-week-old Sprague Dawley (SD) rats were randomly divided into four groups: control group, POF group, MBD treatment group, and Fufang Ejiao Syrup (FES) treatment group. After adaptive feeding for one week, 18 SD rats in the POF, MBD and FES groups were subcutaneously injected with D-galactose (dissolved in saline) at the back of neck for eight weeks (150 mg/kg/day) to establish the POF model. Read More

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January 2019

Therapeutic Effect of the Quinolone Levofloxacin on Inflammatory Fibroblast-like Synoviocytes.

Ann Clin Lab Sci 2019 Jan;49(1):9-15

General Hospital of North China Petroleum Administration Bureau, Renqiu, China.

Objective: This study aims to analyze the effects of quinolones on synovium.

Methods: The present study explored the effects of levofloxacin on fibroblast-like synoviocyte (FLS) . After incubation with 10 ng/mL of interleukin-1β (IL-1β) for 24 hours, rabbit FLS were treated with serial concentrations of levofloxacin (0,14,28,56,112 and 224 μM) for 24 hours. Read More

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January 2019

IGF-1 Promotes Endocytosis of Alveolar Epithelial Cells through PI3K Signaling.

Ann Clin Lab Sci 2019 Jan;49(1):3-8

Department of Immunology, Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, China

Airway inflammation can be mitigated when apoptotic cells are engulfed by pulmonary epithelial cells. Insulin-like growth factor 1 (IGF-1), a single chain polypeptide growth factor, is the main mediator of growth hormone activity in vivo. IGF-1 has many biological activities, such as the regulation of cell survival, proliferation, differentiation and metabolism. Read More

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January 2019
2 Reads

Validation of a Screening Method for Dynamic Mutations in the Gene.

Ann Clin Lab Sci 2018 Nov;48(6):810-813

Faculty of Dental Medicine and Health, J. J. Strossmayer University of Osijek, Croatia.

The objectives of this study were to validate the direct triplet-primed PCR method (dTP-PCR) for determination of dynamic mutations in the gene, and to compare the results of the dTP-PCR method and Southern blot analysis. The number of CGG repeats in the gene was determined by the direct triplet-primed PCR method and by melting curve analysis. The cut-off temperature between normal and permutations of the CGG repeats was determined using control samples with a known number of CGG repeats. Read More

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November 2018
10 Reads

Whole-exome Sequencing Identified a Novel Frameshift Mutation of in a Chinese Family with Neurofibromatosis Type 1.

Ann Clin Lab Sci 2018 11;48(6):808-809

Department of Respiratory Medicine, Diagnosis and Treatment Center of Respiratory Disease, Diagnosis, and Treatment Center of Respiratory Disease, the Second Xiangya Hospital of Central South University, Changsha, Hunan, China

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November 2018

Atypical Acute Monocytic Leukemia: a Lymphoma Presentation and Association with Complex Karyotype.

Ann Clin Lab Sci 2018 Nov;48(6):805-807

Department of Science and Education, Affiliated Hospital of Engineering University of Hebei, Hebei Province, China

A 71-year-old woman presented with acute monocytic leukemia with lymphoma-like morphologic features and an unusual complex karyotype. Bone marrow (BM) aspiration revealed acute monocytic leukemia with lymphoma-like morphologic features. Thus, we called the condition a lymphoma presentation. Read More

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November 2018
2 Reads

Association of the Body Mass Index with the Presence of Gastroesophageal Varices in Compensated Cirrhotic Patients with Hepatitis C Viral Infection.

Ann Clin Lab Sci 2018 Nov;48(6):801-804

Division of Hepatobiliary and Pancreatic Disease, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan.

Obesity is regarded as a risk factor for various benign and malignant diseases. We evaluated whether or not the body mass index (BMI) was associated with the presence of gastroesophageal varices in asymptomatic hepatitis C virus (HCV)-related compensated cirrhosis (Child-Pugh grade A status). Among a total of 794 patients of HCV-related chronic liver disease, 90 had histologically-proven cirrhosis, and 63 were classified as having compensated cirrhosis (30 had varices, and the remaining 33 did not). Read More

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November 2018

A Case of Suspected Streptococcus Pneumoniae Hemolytic Uremic Syndrome (pHUS) with Utilization of Minor Crossmatching for Platelet Blood Products Lead to a Diagnosis of Atypical Hemolytic Uremic Syndrome (aHUS).

Ann Clin Lab Sci 2018 Nov;48(6):797-800

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center McGovern Medical School, Houston, TX, USA.

Background: The action of bacterial neuraminidase of (SPN) results in exposure of the normally "hidden" Thomsen-Freidenreich antigen (T-antigen) found on erythrocytes and other tissues. This may lead to SPN-induced hemolytic uremic syndrome (pHUS) with subsequent hemolysis and end organ damage. pHUS can be identified by minor crossmatch incompatibility. Read More

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November 2018
29 Reads

Synchronous Diagnosis of Acute Myeloid Leukemia with inv(16)(p13q22) and Chronic Lymphocytic Leukemia: A Case Report and Review of the Literature.

Ann Clin Lab Sci 2018 Nov;48(6):790-796

Department of Pathology and Laboratory Medicine, Western Connecticut Health Network, Danbury, CT, USA.

The occurrence of acute myeloid leukemia (AML) with chronic lymphocytic leukemia (CLL) is rare. Most cases of hematologic malignancies such as AML occurring in patients with pre-existing CLL are therapy-related. In this report, we describe a 65-year-old male with no past history of a hematolymphoid malignancy, who presented with abdominal pain. Read More

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November 2018
6 Reads

Rare Association of Mucolipidosis III alpha/beta with Dilated Cardiomyopathy.

Ann Clin Lab Sci 2018 Nov;48(6):785-789

Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea

Mucolipidosis III alpha/beta (ML III alpha/beta) is an autosomal recessive lysosomal storage disorder caused by N-acetylglucosamine-1-phosphotransferase (GlcNAc-phosphotransferase) deficiency. It is characterized by coarse facial features, developmental delay, short stature, and skeletal deformities. Its cardiovascular symptoms include valvular thickening or hypertrophic cardiomyopathy. Read More

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November 2018
13 Reads

A Phenotypically Unusual Hemoglobin H Disease Resulting from a Rare α-Globin Genotype (-/-α).

Ann Clin Lab Sci 2018 Nov;48(6):782-784

Department of Laboratory Medicine, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, China

Hemoglobin H (Hb H) disease is usually characterized by the existence of Hb H, which influences the degree of functional anemia. We here report a patient with a rare Hb H disease genotype (-/-α), who was observed to paradoxically have no detectable Hb H fraction on electrophoresis. To date, the reason why the quantity of Hb H component and the clinical presentation in Hb H disease vary widely is still incompletely understood. Read More

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November 2018
3 Reads

Oculodentodigital Dysplasia with a Novel Mutation in Diagnosed by Targeted Gene Panel Sequencing: A Case Report and Literature Review.

Ann Clin Lab Sci 2018 11;48(6):776-781

Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Oculodentodigital dysplasia (ODDD; MIM #164200), a rare genetic disorder characterized by abnormal craniofacial, dental, ocular, and digital features, is caused by mutations in the gap junction alpha-1 () gene. We report a case of a 6-year-old male who presented with dysmorphic facial features (short palpebral fissure, thin nose with hypoplastic alae nasi, and flat face), bilateral syndactyly, abnormal dentition, and proportionate short stature with growth hormone deficiency. A novel de novo heterozygous missense mutation (c. Read More

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November 2018
2 Reads

Periductal Stromal Tumor of the Breast: Case Report and Literature Review.

Ann Clin Lab Sci 2018 Nov;48(6):770-775

Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center McGovern Medical School, Houston, TX, USA.

Periductal stromal hyperplasia is an exceedingly rare biphasic breast tumor with benign ductal elements and spindle-cell stromal proliferation lacking a phyllodes architecture. It is unclear whether this tumor belongs to the spectrum of phyllodes tumor or is a separate entity. We present a 57-year-old woman with a 3. Read More

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November 2018
1 Read

hsa-miR-7 Is a Potential Biomarker for Idiopathic Inflammatory Myopathies with Interstitial Lung Disease in Humans.

Ann Clin Lab Sci 2018 Nov;48(6):764-769

Department of Neurology and VIP Ward, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objectives: microRNAs (miRNAs) have been identified as biomarkers for various diseases. However, the significance of circulating miRNAs for the diagnosis of idiopathic inflammatory myopathies (IIM) is still unknown. In this study, we aim to investigate the significance of miRNAs as potential biomarkers for predicting the patients with IIM and interstitial lung disease (ILD) METHODS: Total RNA was isolated from plasma of 43 IIM patients and 43 healthy people. Read More

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November 2018
0.839 Impact Factor

The Relationship between Serum Total Bilirubin and Carotid Intima-Media Thickness in Patients with Prehypertension.

Ann Clin Lab Sci 2018 Nov;48(6):757-763

Hypertension Research Laboratory of Guangdong, Cardiovascular Institute, Guangzhou, China

Aim: It has been found that serum total bilirubin has a vital role in anti-inflammatory and antioxidant progress. Prehypertension, the intermediate stage between normal blood pressure and hypertension, is linked with subclinical atherosclerosis and target-organ damage.

Methods: This study was designed to evaluate the relationship between total bilirubin and carotid intima-media thickness (cIMT), a marker of subclinical atherosclerosis, in patients with prehypertension. Read More

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November 2018
1 Read

A Clinical Trial on Oral Infection of Preschool Children.

Ann Clin Lab Sci 2018 Nov;48(6):751-756

Research Lab of Oral H. pylori, Everett, WA, USA

Goals: Over the past twenty years, the existence of oral () infection has been controversial and is still disputed. It proposes that living H. pylori do not exist in the oral cavity. Read More

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November 2018
5 Reads

Role of Bone Marrow Mesenchymal Stromal Cells in Attenuating Inflammatory Reaction in Lipopolysaccaride-induced Acute Kidney Injury of Rats Associated with TLR4-NF-κB Signaling Pathway Inhibition.

Ann Clin Lab Sci 2018 Nov;48(6):743-750

Department of the Intensive Care Unit, The Fourth Affiliated Hospital of Kunming Medical University (The Second People's Hospital of Yunnan Province), China

Bone marrow mesenchymal stromal cells (BMSCs) have positive therapeutic effects on inflammation associated diseases. However, the underlying mechanism is largely unknown. This study was conducted to investigate whether BMSCs could alleviate the inflammation reaction in lipopolysaccaride (LPS)-induced acute kidney injury (septic-AKI) of rats via inhibition of toll-like receptors (TLR4)-nuclear factor-kappa B (NF-κB) signaling pathway. Read More

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November 2018
1 Read

3-Iodothyronamine Acting through an Anti-Apoptotic Mechanism Is Neuroprotective Against Spinal Cord Injury in Rats.

Ann Clin Lab Sci 2018 Nov;48(6):736-742

Orthopedics Department, The Affiliated Hospital of Guizhou Medical University, Guiyang, China

This study aims to show how 3-iodothyronamine (T1AM) protects against spinal cord injury (SCI) in rats. We randomly divided adult female Sprague-Dawley rats (N=54) into three equal groups: (1) untreated control (n=18) (2) T1AM (n=18) (3) T1AM+EPPTB (n=18). The clamp method was used to produce SCI at the T10 segment, and the following data were collected 3, 5, and 7 days after the injury plus treatment. Read More

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November 2018
2 Reads
0.839 Impact Factor

Algorithm to Estimate the Extended Turnaround Time Including Outpatient Waiting Time for Blood Specimen Collection when a Stand-alone Queue Ticket System not Connectable to Laboratory Information System Is Used.

Ann Clin Lab Sci 2018 Nov;48(6):726-735

Department of Laboratory Medicine, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Bucheon, Korea

Background: A queue ticket system (QTS) used in an outpatient phlebotomy clinic was unable to be directly integrated with the laboratory information system (LIS). To monitor patient's waiting time and extended turnaround time (TAT) as patient-centered quality indicators for outpatient laboratory services, we developed an algorithm to integrate data between the QTS and the LIS.

Methods: Between June 1 to September 30, 2017, data files were exported from a QSYS-8000 (HION Tech, Seoul, Korea). Read More

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November 2018
1 Read

Downregulation of miR-196b Promotes Glioma Cell Sensitivity to Temozolomide Chemotherapy and Radiotherapy.

Ann Clin Lab Sci 2018 Nov;48(6):719-725

Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Human glioma is the most common type of primary brain tumor. The survival rate of people with a malignant glioma is extremely low, primarily due to a lack of effective treatments. We previously reported that miR-196b expression is upregulated in glioblastoma tissues and overexpression of miR-196b is associated with poor prognosis. Read More

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November 2018

Upregulated Expression of Serum Exosomal microRNAs as Diagnostic Biomarkers of Lung Adenocarcinoma.

Ann Clin Lab Sci 2018 Nov;48(6):712-718

Stem Cell Clinical Research Center, National Joint Engineering Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China

Lung cancer is the leading cause of cancer-related death worldwide. Lung adenocarcinoma (AC) cases account for approximately 40% of lung cancers. Early diagnosis can reduce mortality, improve prognosis, prolong survival, and improve quality of life. Read More

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November 2018
2 Reads

Increased Expression of Lncrna RP11-397A15.4 in Gastric Cancer and Its Clinical Significance.

Ann Clin Lab Sci 2018 Nov;48(6):707-711

Department of Gastroenterology, People's Hospital of Taizhou, Taizhou, Jiangsu, China

Background: Long noncoding RNAs (lncRNAs) have been demonstrated to have potential diagnostic and prognostic values for many types of cancers. However, their clinical value in patients with gastric cancer (GC) remains unknown.

Methods: The expression levels of lncRNA RP11-397A15. Read More

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November 2018
2 Reads

Prognostic Significance of Categorizing Gastric Carcinoma by PD-L1 Expression and Tumor Infiltrating Lymphocytes.

Ann Clin Lab Sci 2018 Nov;48(6):695-706

Department of Pathology, University of Ulsan College of Medicine, Gangneung, Korea

Goals: We categorized gastric cancers into four types of tumor immune microenvironment based on PD-L1 expression and tumor-infiltrating lymphocytes (TILs) to analyze clinicopathologic characteristics and recommend an immunotherapy-targetable subgroup.

Procedures: Formalin-fixed, paraffin-embedded tissue samples of randomly selected gastric adenocarcinoma (n=479) were obtained and arrayed using a tissue-arraying instrument. Immunohistochemical stains for PD-L1, PD-1, and CD8 were performed and evaluated in addition to microsatellite instability, EBV infection, and HER-2 analyses. Read More

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November 2018
8 Reads

Retraction.

Authors:

Ann Clin Lab Sci 2018 09;48(5):688

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September 2018

Chemical Pathology of Homocysteine VI. Aging, Cellular Senescence, and Mitochondrial Dysfunction.

Authors:
Kilmer S McCully

Ann Clin Lab Sci 2018 Sep;48(5):677-687

Pathology and Laboratory Medicine Service, Boston Veterans Affairs Medical Center, and Department of Pathology, Harvard Medical School, Boston, MA, USA

Following the discovery that caloric restriction extends the lifespan of many species of animals, the free radical theory of aging attributes the occurrence of oxidized nucleic acids, proteins, and lipids to reactive oxygen radical species originating from the metabolism of foods and the diminished efficacy of oxidative metabolism. Because of the decline of many critical neuro-hormones in aging, the neuroendocrine theory of aging attributes these changes to reduced feedback control of hormone production by the hypothalamus. Several rare genetic diseases attribute accelerated aging to changes in deoxyribonucleic acid (DNA) repair, depletion of the coenzyme nicotinamide adenine dinucleotide (NAD), and altered methionine and homocysteine metabolism. Read More

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September 2018
18 Reads

PCR Based Fecal Pathogen Panel Testing Should Be Interpreted with Caution at Diagnosis of Pediatric Inflammatory Bowel Diseases.

Ann Clin Lab Sci 2018 Sep;48(5):674-676

Section of Pediatric Gastroenterology, Texas Children's Hospital, Baylor College of Medicine, Houston TX, USA

Pediatric inflammatory bowel disease (IBD) is in the differential diagnosis for children presenting with chronic diarrhea. During evaluation, stool studies are typically obtained to evaluate for an infectious source, and stool culture has been the conventional testing modality for bacterial pathogens. Recently, there has been increased utilization of molecular testing for enteric pathogens, including multiplex PCR. Read More

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September 2018
20 Reads

Bone Marrow Necrosis in Sickle Cell-Beta Thalassemia Patient Mimicking Thrombotic Thrombocytopenic Purpura.

Ann Clin Lab Sci 2018 Sep;48(5):670-673

Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX, USA

We describe a case of bone marrow necrosis (BMN) with subsequent fat embolism precipitated by high altitudes in a young healthy man with previously undiagnosed sickle cell-beta thalassemia. This case report adds to the growing literature on the complexity of diagnosing bone marrow necrosis given that the clinical and laboratory features mimic thrombotic thrombocytopenic purpura. This BMN with fat embolism syndrome (FES) is novel in that it was precipitated by high altitudes and the patient was treated successfully with a series of therapeutic plasma exchanges and red blood cell transfusions alone, without any adverse long-term complications to the patient. Read More

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September 2018
1 Read

Cutaneous Skeletal Hypophosphatemia Syndrome in Association with a Mosaic Mutation.

Ann Clin Lab Sci 2018 Sep;48(5):665-669

Department of Pediatrics, Seoul National University College of Medicine, Seoul National University Children's Hospital, Seoul, Korea

Recent molecular genetic studies have revealed that Schimmelpenning-Feuerstein-Mims syndrome (SFMS), which presents as sebaceous nevi, is a mosaic RASopathy caused by postzygotic somatic activating mutations in , , or Some patients with SFMS also have hypophosphatemic rickets, called cutaneous skeletal hypophosphatemia syndrome (CSHS). We here report a pediatric case of biopsy-proven CSHS with mosaic mutation in the gene. A girl who showed extensive nevus sebaceous since birth had suffered progressive lower extremity deformity since the age of 5 years. Read More

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September 2018
21 Reads

Morphoproteomics and Biomedical Analytics Identify the c-Myc, EZH2, Sirt1 and CXCR4 Pathways as Potential Blocks to Differentiation Leading to Proliferation and Metastatic Potential in Sinonasal Undifferentiated Carcinoma (SNUC) and Provide Therapeutic Options: A Case Study.

Ann Clin Lab Sci 2018 Sep;48(5):659-664

Biomedical Analytics, Houston, TX, USA.

Sinonasal undifferentiated carcinoma (SNUC) is a highly malignant tumor in the nasal cavity or paranasal sinuses. Morphoproteomics has defined its biology to some degree, allowing the identification of targeted therapeutic options with clinical efficacy [1]. This study's objective was to identify putative SNUC pathways that are known to pose a block in differentiation both in early embryogenesis and in tumorigenesis or that might promote metastasis and recurrent disease. Read More

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September 2018
44 Reads

L-asparaginase: A Novel Bacterial Antigen that May Contribute to Infection Detection.

Ann Clin Lab Sci 2018 Sep;48(5):654-658

Department of Molecular Medicine, Unit of Immunology and General Pathology, University of Pavia, Pavia, Italy

is responsible for gastric inflammation and for an increased risk of cancer development in humans. Several bacterial antigens contribute to stimulate the immune system, but their relative role has not yet been defined. (strain CCUG) type II L-asparaginase (L-ASNase) induces an immune response in mice. Read More

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September 2018
11 Reads

Use of MALDI-TOF MS for Rapid Identification of Clinical Bacterial Strains that Are Uncommon or Difficult to Identify.

Ann Clin Lab Sci 2018 Sep;48(5):646-653

Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, Anhui, China

The purpose of this investigation was to evaluate the analytical and practical performance of MALDI-TOF MS for the identification of clinical uncommon or difficult-to-identify bacteria. A total of 180 clinical isolates collected from January 2014 to December 2016, were identified by MALDI-TOF MS and Vitek2 Compact using 16S rRNA gene sequence analysis as the gold method. For MALDI-TOF MS, 152(84. Read More

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September 2018
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High Mean Platelet Volume Is Associated with Worse Outcomes in Patients with HBV-Related Decompensated Cirrhosis.

Ann Clin Lab Sci 2018 Sep;48(5):639-645

Department of Gastroenterological Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang

Background: Mean platelet volume (MPV) is an inexpensive, routinely measured clinical parameter, and its elevation shows an increase in the size and activity of platelets. It has been shown that MPV can be used as a prognostic biomarker in certain chronic diseases. In the present study, MPV in patients with hepatitis B virus-related decompensated cirrhosis (HBV-DeCi) was investigated to evaluate whether MPV may have utility as a new predictive marker for mortality in HBV-DeCi patients. Read More

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September 2018
11 Reads