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Ann Clin Biochem 2022 May 14:45632221102767. Epub 2022 May 14.

Department of Biochemistry, 410730All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.

Ann Clin Biochem 2022 May 12:45632221103805. Epub 2022 May 12.

71068Sun Yat-sen University First Affiliated Hospital.

Background: Acute respiratory distress syndrome (ARDS) is a severe disease with high mortality, and its primary cause is sepsis. The aim of this study was to detect and evaluate the role of Human epididymis protein 4 (HE4) in sepsis-related ARDS.

Methods: One hundred and twenty-three critically ill sepsis patients with/without ARDS and 102 healthy controls were enrolled in this study. Read More

Ann Clin Biochem 2022 May 15:45632221100347. Epub 2022 May 15.

4595Liverpool University Hospital NHS Foundation Trust, Liverpool, UK.

The introduction of high sensitivity measurement of cardiac troponin T (hs cTnT) and cardiac troponin I (hs cTnI) has given the laboratory the ability to measure very low levels of cardiac troponin. The limit of detection of these assays is well below the 99th percentile. These low levels can also be measured with small values of imprecision. Read More

Ann Clin Biochem 2022 May 7:45632221098870. Epub 2022 May 7.

Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, Toyoake, Japan.

Objectives: High concentrations of low-density lipoprotein cholesterol (LDL-C) are a risk factor for cardiovascular disease. We validated the efficacy of the Martin method is useful in the estimation of LDL-C concentrations was validated in Japanese populations and derived a modified Martin method for easy laboratory information system applications.

Methods: We created 3 subject groups, including 2664 health check-up participants registered with the Resource Center for Health Science, 29,806 clinical patients (A) in the Gifu University Hospital, and 113,716 clinical patients (B) in the Fujita Health University Hospital. Read More

Ann Clin Biochem 2022 May 5:45632221098628. Epub 2022 May 5.

Department of Clinical Biochemistry, Kelvin Laboratory, Royal Victoria Hospital, 1602Belfast Health and Social Care Trust (BHSCT), Belfast, UK.

Background: The widely automated method using indirect ion specific electrodes (ISE) potentiometry for determination of sodium concentration is prone to interference from lipaemia. Manufacturer-specified lipaemic (L)-index cut offs may underestimate the effects of endogenous lipaemia.

Methods: We assessed the interference on sodium concentration caused by endogenous lipaemia in 32 residual samples (from 13 patients) using indirect ISE (Cobas 8000 modular analyser with c702 module, Roche diagnostics) and direct ISE (GEM 4000 premier, Werfen) potentiometric methods. Read More

Ann Clin Biochem 2022 May 5:45632221096036. Epub 2022 May 5.

Department of Clinical Biochemistry, Royal Surrey County Hospital, Berkshire and Surrey Pathology Services, Guildford, UK.

Background: Faecal Immunochemical tests (FITs) in the assessment of patients presenting with symptoms have generally used a single sample. Little evidence pertains to the use of replicate, where a number of tests are done prior to decision-making or repeat FIT, where additional FIT are performed following clinical decision-making. Overwhelmingly, research has focussed on FIT to help identify colorectal cancer (CRC). Read More

Ann Clin Biochem 2022 May 5:45632221095490. Epub 2022 May 5.

Central Diagnostic Laboratory, 8124University Medical Center Utrecht, Utrecht, The Netherlands.

Background: The peptide hormone hepcidin-25 plays an important role in iron metabolism. Low or high levels of hepcidin-25 are associated with various iron disorders; therefore, hepcidin-25 is an important biomarker. This study describes an easy and fast analytical assay for the quantification of hepcidin-25 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Read More

Ann Clin Biochem 2022 Apr 8:45632221090896. Epub 2022 Apr 8.

Department of Clinical Biochemistry, 8948King's College Hospital NHS Foundation Trust, London, UK.

Ann Clin Biochem 2022 Apr 25:45632221087132. Epub 2022 Apr 25.

Department of Clinical Chemistry and Haematology, 3573Groene Hart Ziekenhuis, Gouda, the Netherlands.

Determination of plasma vitamin B12 (B12) is a frequently requested laboratory analysis, mainly employed to establish B12 deficiency. However, an increased level of B12 is a common unexpected finding that may be related to an increased concentration of one of the B12 binding proteins, haptocorrin or transcobalamin. This paper describes the extensive laboratory evaluation of a patient with an elevated level of plasma B12 with various well-established assays. Read More

Ann Clin Biochem 2022 Apr 14:45632221090837. Epub 2022 Apr 14.

Department of Clinical Biochemistry, 4593Alder Hey Children's NHS Foundation Trust, Liverpool, UK.

Ann Clin Biochem 2022 Mar 25:45632221090410. Epub 2022 Mar 25.

98540St James's University Hospital, Leeds, UK.

Ann Clin Biochem 2022 Mar 2:45632221080897. Epub 2022 Mar 2.

Centre for Research Into Cancer Prevention and Screening, 85326University of Dundee School of Medicine, Dundee, UK.

Background: Polypectomy may be performed at colonoscopy and then subsequent surveillance undertaken. It is thought that faecal haemoglobin concentration (f-Hb), estimated by quantitative faecal immunochemical tests (FIT), might be a useful tumour marker.

Methods: Consecutive patients enrolled in colonoscopy surveillance were approached at two hospitals. Read More

Ann Clin Biochem 2022 May 27;59(3):159-161. Epub 2022 Feb 27.

Department of Clinical Biochemistry, University Hospitals Dorset & Bournemouth University, Dorset, UK.

Ann Clin Biochem 2022 Feb 27:45632221077865. Epub 2022 Feb 27.

Department of Endocrinology, Faculty of Medicine, 36925Saint-Joseph University, Beirut, Lebanon.

Background: For a better assessment of thyroid function, each laboratory should establish its own reference intervals (RI). In Lebanon, no previous study has been conducted to establish the reference values for thyroid function tests.

Methods: This cross-sectional study included 301 volunteers aged between 18 and 65 years (65. Read More

Ann Clin Biochem 2022 Feb 27:45632221080680. Epub 2022 Feb 27.

Nuffield Department of Primary Care Health Sciences, University of Oxford, Oxford, UK.

Ann Clin Biochem 2022 Feb 24:45632221077183. Epub 2022 Feb 24.

Department of Pharmacy, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China.

Background: Vancomycin, norvancomycin, methotrexate, paclitaxel, and imatinib are five commonly used drugs which are all recommended to therapeutic drug monitoring in clinical settings. However, the blood concentration monitoring of these drugs and the interpretations of the test results are limited to some extent due to the differences of testing instruments and testing methods.

Methods: We established an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method for simultaneous quantification of vancomycin, norvancomycin, methotrexate, paclitaxel, and imatinib in human plasma. Read More

Ann Clin Biochem 2022 May 3:45632221086468. Epub 2022 May 3.

Department of Laboratory Medicine, West China Hospital, 34753Sichuan University, China.

Background: Risk management strategies have been proposed for applications in clinical laboratories to reduce patient risks; however, effective and visual risk-monitoring tools are currently lacking in medical laboratories. In this study, we constructed a risk quality control (QC) chart based on risk management strategies.

Methods: We calculated the risk levels of QC materials based on Bayes' theorem by combining the total allowable error, QC results, and the maximum number of unacceptable errors in the laboratory. Read More

Ann Clin Biochem 2022 Mar;59(2):152

Ann Clin Biochem 2022 May 21;59(3):157-158. Epub 2022 Feb 21.

Division of Community and Family Medicine, Center for Community Medicine, 12838Jichi Medical University, Tochigi, Japan.

Ann Clin Biochem 2022 May 8;59(3):205-210. Epub 2022 Feb 8.

Department of Clinical Blood Sciences, South West London Pathology, 4968St George's University Hospitals NHS Foundation Trust, London, UK.

Although enzymatic creatinine methods are subject to fewer interferences than traditional Jaffe creatinine methods, every method in clinical chemistry has limitations. We report, for the first time in the literature, a case of an immunoglobulin M (IgM) paraproteinaemia causing an undetectably low creatinine result on the Roche enzymatic assay. This interference did not occur with other enzymatic creatinine methods produced by Abbott and Siemens or the Roche Jaffe, VITROS dry slide and liquid chromatography with tandem mass spectrometry (LC-MS/MS) creatinine methods. Read More

Ann Clin Biochem 2022 May 30;59(3):211-212. Epub 2022 Jan 30.

Clinical Biochemistry, 2241East Kent Hospitals University NHS Foundation Trust, Kent, UK.

Ann Clin Biochem 2022 May 28;59(3):212-213. Epub 2022 Jan 28.

Cancer Molecular Diagnostics, St James's Hospital, Dublin, Ireland.

Ann Clin Biochem 2022 03 21;59(2):97-100. Epub 2022 Jan 21.

Department of Clinical Biochemistry and NHS Bowel Cancer Screening South of England Hub, Royal Surrey County Hospital, Berkshire and Surrey Pathology Services, Guildford, Surrey, UK.

Ann Clin Biochem 2022 Mar 11:45632221076349. Epub 2022 Mar 11.

Department of Rheumatology, Royal Prince Alfred Hospital and School of Medicine, The University of Sydney, Australia.

Background: The Royal College of Pathologists of Australasia Quality Assurance Programs runs a Quality Assurance Program for the assessment of synovial fluid crystals. It provides aliquots of synovial fluid to various laboratories. The quality of specimens can deteriorate prior to being examined. Read More

Ann Clin Biochem 2022 May 2;59(3):199-204. Epub 2022 Mar 2.

Department of Clinical Chemistry and Hematology, 8185Máxima MC, Veldhoven, The Netherlands.

Background: During normal pregnancy, the glomerular filtration rate (GFR) increases dramatically. Failure to obtain this physiological increase is an important risk factor for morbidity and mortality for both mother and child. The estimated GFR (eGFR) using serum creatinine levels is unsuitable for accurate measurement of renal function during pregnancy. Read More

Ann Clin Biochem 2022 Mar 3:45632221076771. Epub 2022 Mar 3.

Academic Unit of Surgery, School of Medicine, 3526University of Glasgow, Glasgow, UK.

Background: The faecal immunochemical test (FIT) has proven utility for colorectal cancer detection in symptomatic patients. However, most patients with a raised faecal haemoglobin (f-Hb) do not have colorectal cancer. We investigated alternative diagnoses and demographics associated with a raised f-Hb in symptomatic patients. Read More

Ann Clin Biochem 2022 May 4;59(3):183-192. Epub 2022 Feb 4.

Division of Chemical Pathology, Department of Pathology, Stellenbosch University and National Health Laboratory Service, Tygerberg Hospital, Cape Town, South Africa.

Background: Hormone therapy in transgender individuals may impact processes that lead to changes in biochemical analytes, and therefore reference intervals. Currently, few reference interval studies are available for the transgender population. We determined biochemical reference intervals for transgender individuals receiving hormone therapy. Read More

Ann Clin Biochem 2022 May 8;59(3):193-198. Epub 2022 Feb 8.

Department of Clinical Laboratory, 73444Kitasato University Hospital, Sagamihara, Japan.

Background: Accumulating evidence shows that contamination of blood samples by atmospheric ammonia affects blood ammonia test levels; however, reports on the effect of ammonia contamination of assay reagents are limited. Here, we aimed to clarify the effect of ammonia contamination of assay reagents, particularly the therapeutic drug monitoring (TDM) reagents, on the detection levels of blood ammonia using enzymatic assays.

Methods: Ammonia gas was measured in the refrigerator compartment of the automatic analyser and the reaction tank water, probe wash water and drain outlets connected to the automatic analyser. Read More

Ann Clin Biochem 2022 Feb 21:45632211066334. Epub 2022 Feb 21.

Department of Insured Medical Care Management, Graduate School of Medical and Dental Science, 92190Tokyo Medical and Dental University (TMDU), Tokyo, Japan.

Background: Small dense low-density lipoprotein a smaller and denser subfraction among whole low density lipoprotein particles is known to be highly atherogenic. The reference interval (RI) is not strictly defined for serum concentration of sdLDL-cholesterol (sdLDL-C) in Japan. The purpose of this study is to set the reference interval for sdLDL-C in healthy subjects. Read More

Ann Clin Biochem 2022 May 6;59(3):178-182. Epub 2022 Jan 6.

Clinical Biochemistry, New Cross Hospital, 592016Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK.

Background: In samples from patients administered rasburicase, uricolysis leads to spuriously low uric acid results. The manufacturer's recommendation of storing the sample in ice-water until analysis, however, does not fully arrest uricolysis. Since uricase activity is affected by pH and metal chelators, we assessed uricolysis inhibition in sodium fluoride-ethylenediaminetetraacetic acid (EDTA)-citrate sample tube (FC Mix tube, Greiner) used primarily for plasma glucose. Read More