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Ann Clin Lab Sci 2022 03;52(2):339-343

Department of Biochemistry, Division of Cardiology, Seoul St. Mary's Hospital, Seoul, Korea

Ann Clin Lab Sci 2022 Mar;52(2):336-338

Laboratory Services, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA.

Objective: Ashwagandha, an Indian Ayurvedic medicine is indicated to prevent COVID-19 infection. Because IL-6 and C-reactive protein are widely measured to determine risk of cytokine storm in hospitalized COVID-19 patients, we studied potential interference of ashwagandha on these two assays. Previous studies indicated that ashwagandha may interfere with digoxin assay, so we also studied potential interference with digoxin assay. Read More

Ann Clin Lab Sci 2022 Mar;52(2):332-335

Department of Laboratory Medicine, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

Objective: Although real-time reverse transcription-PCR (RT-PCR) is the gold standard for diagnosing coronavirus disease 2019 (COVID-19), simpler and faster antibody detection tests can be complementary for diagnosis of COVID-19. To manage the COVID-19 pandemic, the need for serologic testing has increased. In this report, the newly developed antibody detection assays ACCEL ELISA COVID-19 (ACCEL) and Elecsys anti-SARS-CoV-2 (Elecsys) were evaluated. Read More

Ann Clin Lab Sci 2022 Mar;52(2):323-331

Department of Pathology, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, Korea

Objective: Pancreatic ductal adenocarcinoma (PDAC) and extrahepatic bile duct carcinoma (EBDC) are distinct entities with different clinicopathological implications. Therefore, research to differentiate between the two diseases is compulsory. In this study, four biomarkers were selected (Hippocalcin-like 1 (HPCAL1); annexin A10 (ANXA10); MUC5AC; sodium/potassium-transporting ATPase subunit beta-1 (ATP1B1)) and focus was placed on clarifying the diagnostic performance of each biomarker and pioneering novel-combined biomarker panels to discriminate between PDAC and EBDC. Read More

Ann Clin Lab Sci 2022 Mar;52(2):314-322

Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

Objective: The aim of this study was to investigate the correlation between instrumental and morphological cell differential methods in body fluid (BF) samples.

Methods: Forty ascitic (AF) and forty cerebrospinal (CSF) fluid samples were measured with Sysmex XN1000 and XN2000 instruments in BF mode. Flow cytometry (FC) was carried out with FACS Canto II. Read More

Ann Clin Lab Sci 2022 Mar;52(2):301-313

Graduate School, Hunan University of Chinese Medicine.

Objective: Genkwanin is a biologically active O-methylated flavone extracted from . An increasing number of studies have described the modulatory effects of genkwanin on human diseases, including antitumor, anti-inflammatory, and antioxidant activities. However, little is known about whether genkwanin might be a therapeutic agent for inflammatory bowel disease or its possible underlying mechanisms. Read More

Ann Clin Lab Sci 2022 Mar;52(2):292-300

Department of General Surgery, Chinese PLA General Hospital, Beijing, China

Objective: Aberrantly expressed lncRNAs have been detected in gastric cancer (GC). LncRNA is involved in numerous types of human malignant tumor. In this project, we demonstrated the relationship between and and tested the function of and hsa-miR-30a-3p in the tumorigenesis of GC. Read More

Ann Clin Lab Sci 2022 Mar;52(2):278-291

Department of Obstetrics, Sanmen People's Hospital of Zhejiang Province, Sanmen, China

Objective: We investigated the role and mechanism of GATA-binding factor 2/Fibroblast growth factor 21 (GATA2/FGF21) axis in high glucose (HG)-induced injury in human umbilical vein endothelial cells (HUVEC).

Methods: After HG treatment and transfection, the viability and apoptosis of HUVECs were determined via Cell Counting Kit-8 and Hoechst 33258 staining methods, and the content of lactate dehydrogenase (LDH) and reactive oxygen species (ROS) was measured via colorimetric assay and DCFH-DA staining. The potential transcription factor of FGF21 was predicted with bioinformatic analysis and confirmed via dual-luciferase reporter assay and chromatin immunoprecipitation. Read More

Ann Clin Lab Sci 2022 Mar;52(2):269-277

Department of Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China.

Objective: We aimed to explore the association between manganese (Mn) and the progression of invasive adenocarcinoma of the cervix (IAC), together with the corresponding mechanisms.

Materials: Venous blood was collected from 65 IAC patients and 65 normal controls (female, participated in physical examination) in the fasting state. The levels of Mn and of inflammatory factors in the venous blood were measured using graphite furnace atomic absorption spectrometry (GAS) and enzyme-linked immunosorbent assay (ELISA), respectively. Read More

Ann Clin Lab Sci 2022 Mar;52(2):260-268

Department of Urology, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China

Objective: Bladder cancer (BC) is the most common cancer in urinary system. Recently, the function of family with sequence similarity 107 member A (FAM107A) has been reported in several carcinomas. This study aimed to reveal the potential role of FAM107A in bladder cancer. Read More

Ann Clin Lab Sci 2022 Mar;52(2):249-259

Department of Gastrointestinal Surgery, Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China

Objective: To study the role of long non-coding RNA (lncRNA) CRYM-AS1 in human gastric cancer.

Methods: Expression levels of CRYM-AS1 in cell lines and clinical tissues were examined by RT-qPCR. The association between CRYM-AS1 levels and clinicopathological parameters/survival rates of gastric cancer patients was analyzed. Read More

Ann Clin Lab Sci 2022 Mar;52(2):240-248

Department of Blood Transfusion, Hunan Cancer Hospital, Changsha, China

Objective: Esophageal cancer is a common gastrointestinal tumor, with high incidence in our country. Histone demethylase 4 plays an important role in chromosome structural modification and gene expression regulation, becoming a new target for tumor treatment. GASC1 is an important member of the KDM4 family, closely related to the malignancy of tumors. Read More

Ann Clin Lab Sci 2022 Mar;52(2):230-239

Department of Pathology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, Fujian, China.

Objective: Thymosin b10 (TMSB10), a member of the thymosin family, is mainly located in cells and participates in the assembly and occurrence of cytoskeleton. We aimed to investigate the regulatory mechanism of TMSB10 in ccRCC.

Methods: In this study, Xiantao Academic Tools were taken to perform the pan-cancer expression and immune infiltration analysis of TMSB10. Read More

Ann Clin Lab Sci 2022 Mar;52(2):222-229

Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Mexico, USA

Objective: It has been demonstrated that acetylsalicylic acid (ASA) treatment halves hepatitis C virus (HCV) expression in hepatocarcinoma cells. However, the signaling pathway that promotes this ASA-induced antiviral effect has not yet been identified.

Aim: The aim of this work was to identify alterations in the transcriptional profile of Huh-7-HCV-subgenomic replicon cells with vs. Read More

Ann Clin Lab Sci 2022 Mar;52(2):213-221

Department of Radiotherapy, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Objective: This study aims to investigate how miR-561-5p regulated the expression of RAC1 and whether its effects on RAC1 was associated with the proliferation, migration, invasion, and apoptosis in pancreatic ductal adenocarcinoma (PDAC) cells.

Materials And Methods: RT-qPCR was performed to assess miR-561-5p expression in human PDAC tissues. A series of experiments including cell counting Kit-8, colony formation, cell migration and invasion, and apoptosis assays were used to assess the PDAC cell biological behaviors. Read More

Ann Clin Lab Sci 2022 Mar;52(2):202-212

Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin, China

Objective: To uncover the expression of Lamins B2 (LMNB2) in tumor tissues and the effects on the progression of esophageal cancer.

Materials And Methods: IHC assays were performed to detect the expression of LMNB2 in esophageal cancer tissues. Kaplan-Meier survival analysis was performed to confirm its effects on patients' prognosis. Read More

Ann Clin Lab Sci 2022 Mar;52(2):196-201

Department of Molecular Biology and Genetics, Dicle University, Sur/Diyarbakir, Turkey

Objective: Prostate cancer is one of the most frequently diagnosed cancer in men and ranks as the second most common cause of cancer-related deaths in developed countries. HN1 is a highly expressed gene in prostate cancer and controls the levels of several cell cycle regulatory proteins including Cyclin B1. Cyclin B1 is a cell cycle control protein but is also involved in Docetaxel and 2-Methoxyestradiol induced apoptosis. Read More

Ann Clin Lab Sci 2022 Mar;52(2):185-195

Department of Gastrointestinal Surgery, Shaoxing Second Hospital, Shaoxing, Zhejiang, China

Objective: This study was designed to visualize the pan-cancer prognostic significance of PReferentially expressed Antigen in Melanoma (PRAME) and investigate the relationship between PRAME expression and tumor immunity.

Material And Methods: We explored the expression patterns and prognostic value of PRAME across multiple cancers using data from the Cancer Genome Atlas, Genotype-Tissue Expression, and Cancer Cell Line Encyclopedia databases. Spearman's correlation test was used to evaluate correlations between PRAME expression and the tumor immune microenvironment, mutation indicators, and DNA methylation. Read More

Ann Clin Lab Sci 2022 Mar;52(2):179-184

Department of Pathology, Division of Clinical Laboratories and Molecular Medicine, Massachusetts General Hospital, Boston, MA, USA

Objective: In patients with early Lyme disease (ELD), cardiac involvement is known to occur in approximately 1% of patients. We measured high-sensitivity troponin T (hsTnT) in patients with early Lyme disease to evaluate the possibility of subclinical cardiac involvement.

Methods: We measured hsTnT in 41 patients with well characterized ELD. Read More

Ann Clin Lab Sci 2022 Mar;52(2):170-178

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.

Objective: Found in Inflammatory Zone 1 (FIZZ1) protein plays an important enhancive role in inflammation and angiogenesis. This study aims to explore the effects of FIZZ1 on murine atherosclerosis.

Methods: The murine aortic endothelial cells were treated with an adenoviral vector encoding FIZZ1 short hairpin RNA (Ad-shFIZZ1). Read More

Ann Clin Lab Sci 2022 Jan;52(1):166

Ann Clin Lab Sci 2022 Jan;52(1):161-163

Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston, USA.

Objective: Interleukin -6 (IL-6) is an important diagnostic test in COVID-19 patients to determine whether to initiate tocilizumab therapy or mechanical ventilation. We investigated potential interference of biotin in Roche IL-6 assay which utilizes biotinylated antibody.

Methods: We prepared three serum pools from left-over specimens which showed IL-6 values over 40 pg/mL. Read More

Ann Clin Lab Sci 2022 Jan;52(1):154-160

Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China

Objective: Adenosine deaminase (ADA) plays a major role in maintaining metabolic homeostasis via catalysis of hydrolytic deamination of adenosine to inosine. The ADA1 isoenzyme of ADA is an analyte tested in clinical laboratories; however, lack of quality control (QC) material in terms of enzyme homogeneity, stability, and coverage of the clinically relevant analytical measurement range (AMR), poses a challenge for adequate monitoring of this analyte. The aim of this study was to address the need for manufacture of QC material through recombinant expression of catalytically active ADA1 in eukaryotic cells ( GS115). Read More

Ann Clin Lab Sci 2022 Jan;52(1):141-153

Department of Rheumatology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Hainan, China

Objective: This study aims to explore key candidate genes and predict potential therapeutic agents for rheumatoid arthritis (RA).

Methods: Differentially expressed genes (DEGs) of synovial tissue in patients with RA compared with normal donors are identified by analyzing four expression profiles. Coexpressed DEGs are confirmed by Venn diagrams. Read More

Ann Clin Lab Sci 2022 Jan;52(1):133-139

Division I of in vitro Diagnostics for Infectious Diseases, Institute for in vitro Diagnostics Control, National Institutes for Food and Drug Control, Beijing, China

Objective: We compared the prognostic factors and clinical characteristics of chronic hepatitis B (CHB) patients who received nucleos(t)ide analogues (NAs) therapy to those who did not.

Method: A total of 315 CHB patients were enrolled in this study and were divided into NA (n=144) and non-NA (n=171) groups based on their therapy.

Results: The risk of hepatocellular carcinoma (HCC) development and mortality in the NA group were significantly lower than those in the non-NA group. Read More

Ann Clin Lab Sci 2022 Jan;52(1):126-132

Department of Laboratory Medicine, Myongji Hospital, Hanyang University Medical Center, Goyang, Korea

Objective: The ROP-ESAT6-CFP10 antigen (Changzhou Niujin Shisong Biotech [CBI], China) was recently developed using recombinant overlapping peptide (ROP) technology. We used ROP-ESAT6-CFP10 as a tuberculosis (TB)-specific antigen and compared it with existing interferon-gamma release assays (IGRAs).

Methods: Healthy volunteers and patients who were diagnosed with TB within a one-year period were enrolled. Read More

Ann Clin Lab Sci 2022 Jan;52(1):117-125

Department of Urology, Fuyang People's Hospital, Fuyang, China.

Objective: Previous research showed that ALG3 was associated with several cancers, but the function of ALG3 in bladder cancer (BC) was yet unknown. The purpose of this study was to investigate the relative expression of ALG3 in BC tissues and corresponding normal tissues and the relationship between the relative expression of ALG3 and clinical outcome in bladder cancer patients.

Methods: In this study, the expression of ALG3 in bladder cancer was detected by immunochemistry. Read More

Ann Clin Lab Sci 2022 Jan;52(1):109-116

Graduate School of Jiamusi University, Jiamusi City, Heilongjiang Province, China.

Objective: To investigate the function and mechanism of circular RNA circ HIPK2 on non-small cell lung cancer cell proliferation and progression.

Methods: Patients with non-small cell lung cancer (n=40) were collected from May 2015 to August 2016.

Results: Circular RNA circ HIPK2 expressions were up-regulated in patients with non-small cell lung cancer, compared with paracancerous tissue. Read More

Ann Clin Lab Sci 2022 Jan;52(1):101-108

Department of Physiology, Basic Medical College, Chongqing Medical University, Chongqing, China

Objective: To investigate the effect of dichloroacetate (DCA) on Wilms' tumor (WT) G401 cells.

Methods: CCK-8 assay was used to detect the influence of DCA on G401 cells viability and 10 mmol/L DCA was selected for subsequent experiments. The expression of glycolysis-related enzymes, such as hexokinase 2 (HK2), pyruvate kinase M2 (PKM2), lactic acid dehydrogenase A (LDHA), pyruvate dehydrogenase kinase 1 (PDK1), and pyruvate dehydrogenase (PDH), were detected by qRT-PCR and western blot. Read More

Ann Clin Lab Sci 2022 Jan;52(1):95-100

Department of General Surgery, Jiujiang University Clinical Medical College, Jiujiang University Hospital, Jiujiang City, Jiangxi Province, China

Objective: To investigate the diagnostic value of tumor markers in peritoneal lavage fluid in the diagnosis of peritoneal metastasis from colorectal cancer.

Methods: One hundred eighty-six patients with colorectal cancer and 15 patients with benign disease who underwent surgical treatment were included. The abdominal cavity and pelvis of the patients were lavaged with 200 ml of normal saline immediately after abdominal cavity incision or pneumoperitoneum establishment. Read More