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Ann Biol Clin (Paris) 2022 Feb;80(1):97-102

Ann Biol Clin (Paris) 2022 Feb;80(1):81-83

Faculty of Sciences and Techniques, University My Slimane, Beni Mellal, Morocco.

Ligneous conjunctivitis (LC) is a rare form of pseudomembranous chronic conjunctivitis caused by a deficiency in plasminogen activity. Due to its rarity, little is known about this disorder. We hereby report a case of ligneous conjunctivitis, describing the clinical findings, the biological diagnosis and the treatment of this rare disease. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):15-27

Service d'endocrinologie, de diabétologie et de nutrition, CHU de Bordeaux, France, UMR1215, CNRS-Université de Bordeaux, France.

Covid-19 infection is a potentially serious disease. Overweight, obesity, and diabetes are comorbidities frequently found in the severe form of the disease. Appropriate nutritional management of the patient is an integral part of care. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):103-104

Ann Biol Clin (Paris) 2022 Feb;80(1):69-73

Service de médecine interne, Hôpital Avicenne GHUPSSD, Assistance Publique-Hôpitaux de Paris, Bobigny, France.

We report a case of acquired von Willebrand syndrome relapse in association with Crohn's disease, in a context of non-compliance in a 85-year-old woman suffering from epistaxis and melena. The acquired von Willebrand syndrome is a rare bleeding disorder. This case underlines the importance of maintaining the corticosteroid therapy in order to prevent the reappearance of autoantibodies and the recurrence of this syndrome. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1)

Ann Biol Clin (Paris) 2022 Feb;80(1):85-90

Laboratoire de dépistage périnatal, Centre de Biologie Pathologie Génétique, CHU, Lille, France, Association ABA, Hôpital Robert-Debré, AP-HP, Paris, France.

Screening for fetal Down's syndrome has the peculiarity of combining the biochemical assay of 2 or 3 serum markers with the risk associated with maternal age. If the accuracy of measurement of each parameter is known by the biologist, the uncertainty of the ultimate risk to the patient is not. Indeed, the means of risk calculation involve numerous multi-parameter equations which are not practical for daily use. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):7-14

C2VN (AMU - Inserm1263 - Inrae 1260), Aix-Marseille Université - Campus Santé Timone - Faculté de Pharmacie, Marseille, France, Laboratory of Biochemistry, Hôpital de La Timone, Marseille, France.

Homocysteine (Hcy) is a sulfhydryl-containing amino acid, which is not acquired through the diet, but rather synthesized as an intermediate metabolite in the methionine cycle. Hcy is present in plasma, with normal levels between 5 and 15 μmol/L, a slightly elevated level between 15 to 30 μmol/L, moderate from 30 to 100 μmol/L and a value > 100 μmol/L classified as severe hyperhomocysteinemia (HHcy). HHcy has been associated with inflammation and atherosclerosis and is considered an independent risk factor for cardiovascular diseases (CVD). Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):29-46

Équipe de bioénergétique et métabolisme intermédiaire, LBPO, FSB, Université des Sciences et de la technologie Houari Boumediene, Alger, Algérie.

The 25-hydroxyvitamin D (25OHD) deficiency in Crohn's disease (CD) is associated with the immune system dysfunction and redox status alteration. These two events affect intestinal mucosal function through macrophages cells infiltration and to lead a pro-inflammatory cytokines storm and ROS (reactive oxygen species) overproduction. The objective of this study was to investigate the immunomodulatory and antioxidant effects of vitamin D supplementation (DS) in clinical active phase. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):65-68

CHU Lille, Service d'hormonologie, métabolisme, nutrition, oncologie, Lille, France.

Management of triglyceride (TG) levels is essential in intensive care units (ICU), especially to manage the risk of pancreatitis induced by propofol. However, some therapeutics in ICU such as intravenous ascorbic acid protocol, especially used in the context of Covid-19 could lead to false decrease of triglycerides by analytical disruption of Trinder reaction. We report here the case of a sample with unmeasurable triglyceride levels partly due to high plasma ascorbic acid levels. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):47-54

Service d'hématologie biologique, CHRU de Nancy, France, Plateforme de cytométrie en flux, CHRU de Nancy, France.

Large granular lymphocytic leukemia (LGLL) is a rare clonal lymphoproliferative disorder from T or NK origin.

Purpose: to report on the diagnostic and therapeutic management of LGLL investigated in the university hospital at Nancy, France.

Methods: retrospective (7 years) collection of clinical and biological data and patients' cohort analysis. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):61-64

Pôle de biologie Santé Site CHSR - CHU Réunion, Laboratoire d'hématologie, Saint-Pierre, La Réunion.

An 88-year-old man is sent to hospital because of a rapid worthening of his general state. A myelogram is obtained following the discovery of hypercalcemia associated with a diminished glomerular filtration rate and the discovery of osteolytic lesions, showing a massive proliferation of plasma cells. The lack of plasma and urine detection of both monoclonal antibodies and immunoglobulin light chains, as well as an immature plasma cell phenotype, suggest the diagnosis of non-secretory multiple myeloma. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):75-79

Laboratoire GCS HospiBIO, Centre hospitalier d'Armentières, France, Faculté de pharmacie, Université de Lille, France.

Heavy chain diseases are rare gammopathies characterized by the production of a truncated heavy chain monoclonal immunoglobulin without associated light chain. These hemopathies, which are frequently associated with immunological or hematological disorders, are clinically closer to lymphomas than to myelomas. The case that we report illustrates the difficulty to characterize a heavy chain gammopathy discovered in an elderly woman admitted in the department of internal medicine for alteration of general condition. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):91-95

Laboratoire de biochimie, DMU biologie et génomique médicales, AP-HP Sorbonne Université, Hôpital Saint-Antoine, Paris.

The first step to complete in a method validation of a biological analysis is the study of precision intra-assay. In the absence or insufficient quantity of control material, this study may be difficult to perform. This article proposes a methodology for preparing its own control samples for the radioimmunological assay of the amino-terminal peptide of procollagene type III (PIIIP). Read More

Ann Biol Clin (Paris) 2022 Feb;80(1):55-60

Laboratoire de biochimie clinique, CHU Mohammed-VI - Marrakech, Maroc.

Capillary serum protein electrophoresis (CPE) is a high-resolution technique. Thus, it is highly sensitive to the detection of qualitatives dysalbuminemia. By the present study, we aim to describe modifications of albumin fraction within the electrophoretogramm and to expose the underlying diseases. Read More

Ann Biol Clin (Paris) 2022 Feb;80(1)

Ann Biol Clin (Paris) 2021 Dec;79(6):613-614

Laboratoire d'Hématologie, AP-HP, Hôpital Bichat Claude-Bernard, Paris, France.

Ann Biol Clin (Paris) 2021 Dec;79(6):603-611

Laboratoire d'hématologie et d'immunologie, Centre Hospitalier Intercommunal Aix-Pertuis, Aix en Provence, France.

Argatroban is indicated in the management of patients with suspicion of Heparin-Induced Thrombocytopenia. Biological monitoring of argatroban can be carried out using the activated partial thromboplastin time (APTT) or the measurement of the anti-IIa activity. However, APTT is susceptible to many interferences making its use unsuitable. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):597-602

Laboratoire d'hématologie, Centre hospitalier universitaire IBN Rochd, Casablanca, Maroc, Université Hassan II, faculté de médecine et de pharmacie, Casablanca, Maroc.

Large granular lymphocyte leukemia (LGL) constitutes a heterogeneous entity with very different immunophenotypic, clonal and evolutionary characteristics. The most common LGL-T are CD3 +, CD8 +, CD16 +, CD57 +, CD56-. The majority of patients have a chronic disease, systemic signs are rare, and symptoms mainly result from neutropenia or associated autoimmune diseases. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):567-578

Service de biochimie, Hôpital universitaire Necker-Enfants malades, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France, Unité de technologies chimiques et biologiques pour la santé (UTCBS), CNRS UMR8258- U1022, Faculté de Pharmacie Paris-Descartes, Université de Paris, France.

An increase of pyroglutamic acid, or 5-oxoproline plasmatic concentration was reported in metabolic acidosis observed after chronic intake of some drugs, as acetaminophen. We developed a simple, fast and reproducible method by capillary zone electrophoresis using a commercial Anion Analysis Kit to quantify pyroglutamic acid, in plasma after acetonitrile precipitation, and after simple dilution in urines. Fumaric acid was used as internal standard in both. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):551-565

Service de médecine génomique des maladies de système et d'organe, Hôpital Cochin, APHP, Centre université de Paris, France.

Palmoplantar keratodermas (PPK) comprise a heterogenous group of acquired and hereditary disorders marked by excessive thickening of the epidermis of palms and soles. Hereditary PPKs can be classified into 3 groups: 1) isolated non-syndromic PPKs; 2) complex non-syndromic PPKs associated with other ectodermal defects; and 3) syndromic PPKs associated with extracutaneous manifestations. All types of inheritance have been observed: autosomal dominant, autosomal recessive, X-linked recessive, and mitochondrial. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):535-549

CHU de Bordeaux, UMR5536 CNRS-université de Bordeaux, laboratoire de biochimie, France.

During the first wave of Covid-19 in France, in spring 2020, healthcare institution's laboratory had to adapt itself quickly to the growing demand for emergency biology, in particular by reorganizing their POCT analyzers: redeployment of analyzers and/or new installations. In order to analyze this management, a subgroup of 15 hospital biologists from the SFBC Working Group "Biochemical markers of Covid-19" sent, in fall 2020, an on-line survey to French hospital laboratories using POCT. Answers analysis (n = 86) shows a territorial disparity related to the severity of the first wave: increased activity essentially in red zones, management of unexpected situations, training of additional nursing staff for 40 % of the laboratories. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):505-532

Laboratoire d'hématologie cellulaire, GHS/Hospices civils de Lyon, Pierre Bénite, France, Équipe Lymphoma Immuno-Biology, Faculté de médecine Lyon-Sud, Pierre-Bénite, France, Groupe CytHem-SLP.

Ann Biol Clin (Paris) 2021 Dec;79(6):579-586

His excellency the Minister of Public Health in the Lebanese government, Ministry of Public Health (MOPH), Bir Hassan, Jnah, Beirut, Lebanon.

Automated immunoassays have been introduced to complement real time PCR (RT-PCR) in the battle against SARS-CoV-2. This study compares four serological automated immunoassays by different manufacturers (Abbott, Euroimmun, Roche and Snibe) that are available in Lebanon with regards to specificities, sensitivities, inter-agreement and positive/negative distinction abilities. One hundred and fifty-seven samples (73 with a positive RT-PCR and 84 controls) were analyzed. Read More

Ann Biol Clin (Paris) 2021 Dec;79(6):587-596

Laboratoire de biologie médicale, Groupe hospitalier Bretagne-Sud, Lorient.

Albumin is a main biomarker of denutrition severity. Since 2019, the French National Authority of Health (HAS) recommends an immunologic technique as a reference for measuring albuminemia. In a context of partial recovery by the laboratory of GHBS (using colorimetric method with bromcresol purple) of the biological activity of the dialysis center, until then carried out by the Ouest biology - Biolor laboratory (using colorimetric method with bromcresol green) and in order to standardize practices in the healthcare sector, we compared 3 albumin assay techniques: immunoturbidimetric (Diagam), BCG (Siemens), BCP (Siemens) on Siemens Atellica PLCs. Read More

Ann Biol Clin (Paris) 2021 Oct;79(5):472-486

Ann Biol Clin (Paris) 2021 Oct;79(5):461-469

Hospices civils de Lyon, Centre hospitalier Lyon-Sud, Centre de biologie Sud, Service de biochimie et biologie moléculaire, Pierre-Bénite, France.

Eosinophiluria corresponds to search eosinophils in urine. These are normally absent in urine, their presence could be associated with different clinical conditions affecting kidney or urinary tract. This analysis of medical biology laboratory listed in the French Nomenclature of acts of medical biology has to be validated according to norm NF EN ISO 15189. Read More

Ann Biol Clin (Paris) 2021 Oct;79(5):456-459

Service de microbiologie clinique, Groupe hospitalier Paris Saint-Joseph, Paris, France.

Babesiosis is a tick-borne infectious disease, caused by an intraerythrocytic parasite of the genus Babesia. It has clinical, biological and microbiological similarities with Plasmodium related infections. In rare cases, babesiosis may be complicated by hemophagocytic lymphohistiocytosis, which occurs preferentially in the immunodeficient patient. Read More

Ann Biol Clin (Paris) 2021 Oct;79(5):445-451

Service de biochimie, CHU de Bordeaux, France, UMR5536, CNRS-Université de Bordeaux, France.

Potential discrepancies between laboratory and estimated (from Continuous Glucose Monitoring (CGM)) glycated hemoglobin (HbA1c) have been reported by diabetologists. CGM devices produce an eA1c derived from average glucose and correlated with Time-in-Range (TIR, %) which is the relative time spent in a range of normal glycaemia. Through a case report, we studied the potential causes for these discrepancies. Read More

Ann Biol Clin (Paris) 2021 Oct;79(5):427-438

Service de biologie de la reproduction, Centre d'AMP du CHU de la Réunion, Saint-Pierre, France.

The aim of this study was to evaluate the ART outcomes for infertile males carrying a balanced structural chromosomal rearrangement or a Y-chromosome microdeletion, and to compare the results with a control group. The primary outcome was the clinical pregnancy rate. A retrospective case-control study has been carried out in the ART departments of the university hospitals of Bordeaux and la Réunion. Read More