6,477,813 results match your criteria Animals : an open access journal from MDPI[Journal]

  • Page 1 of 129557

Verapamil prevents the effect of calcium-sensing receptor activation on the blood glucose and insulin levels in rats.

Pharmacol Rep 2019 Jan 25;71(3):478-484. Epub 2019 Jan 25.

Department of Pathophysiology, Faculty of Pharmacy, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Background: The Ca triggered insulin exocytosis in β cells of the pancreatic islets may be the result of Ca influx through L-type voltage dependent calcium channels (VDCC) localized in the plasma membrane, as well as of liberation of Ca from intracellular storages, induced by activation of the calcium receptor (CaR) coupled with the PLC enzyme present in the pancreatic islets. The present study was designated to determine, in in vivo experiments, the effects of CaR activation by R-568 and inhibition of the receptor by NPS 2143 on the plasma glucose and insulin levels in the presence of verapamil, a calcium channel blocker.

Methods: Wistar rats, after fasting for 14 h before the experiment, were anesthetized with inactin and loaded ip with 1 g/kg glucose. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pharep.2019.01.004DOI Listing
January 2019

Influence of salbutamol on the anticonvulsant potency of the antiepileptic drugs in the maximal electroshock-induced seizures in mice.

Pharmacol Rep 2019 Feb 8;71(3):466-472. Epub 2019 Feb 8.

Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland.

Background: β-Adrenergic receptor agonists are widely used agents in the treatment of asthma or preterm labor. Since prevalence of asthma was shown to be higher in patients with epilepsy and modulation of noradrenergic system activity may modify epilepsy course, the aim of the present study was to examine the effect of salbutamol (SALB), one of the most commonly used β-adrenergic receptor agonist on the anticonvulsant potency of four classical antiepileptic drugs (AEDs): valproate (VPA), carbamazepine (CBZ), phenytoin (DPH) and phenobarbital (PB) in mice subjected to the maximal electroshock (MES)-induced seizures.

Methods: Seizures were caused by a current delivered through ear-clip electrodes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pharep.2019.02.003DOI Listing
February 2019

Gut microbiota: An underestimated and unintended recipient for pesticide-induced toxicity.

Chemosphere 2019 Apr 13;227:425-434. Epub 2019 Apr 13.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, PR China. Electronic address:

Pesticide pollution residues have become increasingly common health hazards over the last several decades because of the wide use of pesticides. The gastrointestinal tract is the first physical and biological barrier to contaminated food and is therefore the first exposure site. Interestingly, a number of studies have shown that the gut microbiota plays a key role in the toxicity of pesticides and has a profound relationship with environmental animal and human health. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00456535193074
Publisher Site
http://dx.doi.org/10.1016/j.chemosphere.2019.04.088DOI Listing
April 2019
1 Read

Bioaccessibility estimates by gastric SBRC method to determine relationships to bioavailability of nickel in ultramafic soils.

Sci Total Environ 2019 Apr 6;673:685-693. Epub 2019 Apr 6.

School of Environmental Sciences, University of Guelph, Guelph, ON, Canada.

Frameworks for human health risk assessment often include the opportunity to correct the estimate of exposure for bioavailability, which could be predicted from bioaccessibility. Lead and As are the only metallic elements for which bioavailability and bioaccessibility have been correlated across a spectrum of mineralogy and particle types. The objective of the present study is to correlate in vivo bioavailability with ex vivo bioaccessibility for elevated Ni in soils of ultramafic origin and explore attribution of any variation in this correlation to mineralogical characterization of the Ni. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.04.059DOI Listing

Characterization of the human central nucleus of the inferior colliculus.

Hear Res 2019 Apr 11;377:234-246. Epub 2019 Apr 11.

Department of Anatomy, Lake Erie College of Osteopathic Medicine, Erie, PA, United States. Electronic address:

The inferior colliculus (IC) is a major relay station for both ascending and descending auditory pathways. The IC is divided into three major regions, the external cortex (ECIC), the dorsal cortex (DCIC) and the central nucleus of the inferior colliculus (CNIC). While the ECIC and DCIC receive many non-auditory inputs, the CNIC receives predominantly auditory input ascending within the lateral lemniscus and descending input from the cerebral cortex. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S03785955183061
Publisher Site
http://dx.doi.org/10.1016/j.heares.2019.04.004DOI Listing
April 2019
1 Read

Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection.

Res Vet Sci 2019 Apr 11;124:263-269. Epub 2019 Apr 11.

Department of Veterinary Science, University of Parma, Strada del Taglio, 10, 43126 Parma, Italy.

Porcine reproductive and respiratory syndrome virus (PRRSV) is known to be clinically responsible for reproductive failure in sows and post-weaning respiratory disease in growing piglets. During the last years, highly pathogenic PRRSV isolates have been discovered. In Italy, a PRRSV-1 subtype 1 strain (namely PR40/2014) characterized by high pathogenicity was isolated and experimental infection was characterized in terms of virological/clinical features and immune modulation (Canelli et al. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00345288183159
Publisher Site
http://dx.doi.org/10.1016/j.rvsc.2019.04.007DOI Listing
April 2019
1 Read

(-)-α-Bisabolol reduces nociception and trigeminal central sensitisation in acute orofacial neuropathic pain induced by infraorbital nerve injury.

Life Sci 2019 Apr 16. Epub 2019 Apr 16.

Faculty of Dentistry, University of Toronto, Canada; Deparment of Physiology, University of Toronto, Canada.

Neuropathic orofacial pain conditions represent a challenge to diagnose and treat. Natural substances are promising therapeutic options for the control of pain.

Aims: This study aimed to examine whether (-)-α-bisabolol (BISA), a natural terpene, can attenuate nociceptive behaviour and central sensitisation in a rodent model of trigeminal neuropathic pain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2019.04.032DOI Listing

Guppies show rapid and lasting inhibition of foraging behaviour.

Behav Processes 2019 Apr 16. Epub 2019 Apr 16.

Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.

To cope with the variable environment, animals are continuously required to learn novel behaviours or, in certain cases, to inhibit automatic and previously learned behaviours. Traditionally, inhibition has been regarded as cognitively demanding and studied mostly in primates, other mammals and birds, using laboratory tasks, such as the cylinder task. Recent studies have also revealed that fish show high levels of inhibition in the cylinder task. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.beproc.2019.04.011DOI Listing

Long-term administration of Aspalathus linearis infusion affects spatial memory of adult Sprague-Dawley male rats as well as increases their striatal dopamine content.

J Ethnopharmacol 2019 Apr 16:111881. Epub 2019 Apr 16.

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1b, 02-097, Warsaw, Poland. Electronic address:

Ethnopharmacological Relevance: Everyday use of the herbal tea rooibos, produced from Aspalathus linearis (Brum.f) Dahlg. (Fabaceae) is customary in South Africa, a continuation of its historical use by indigenous people. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2019.111881DOI Listing

Optical coherence tomography angiography (OCT-A) in an animal model of laser-induced choroidal neovascularization.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Ophthalmology, University of Bonn, Bonn, Germany.

Aim of the study was to compare optical coherence tomography angiography (OCT-A) and conventional fluorescein angiography (FA) for quantitative analysis of the retinal and choroidal vasculature in the animal model of laser-induced choroidal neovascularization (CNV). Therefore, Dark Agouti rats underwent argon laser photocoagulation to induce CNV at D0. In vivo imaging using combined confocal scanner laser ophthalmoscopy (cSLO)-based FA and OCT-A (Heidelberg Engineering GmbH, Heidelberg, Germany) was performed before and immediately after laser treatment as well as at day 2, 7, 14 and 21. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exer.2019.04.002DOI Listing

Morin post-treatment confers neuroprotection in a novel rat model of mild repetitive traumatic brain injury by targeting dementia markers, APOE, autophagy and Wnt/β-catenin signaling pathway.

Brain Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacology & Toxicology, Cairo University, Cairo, Egypt. Electronic address:

Exposure to repetitive brain trauma has gained attention for its similarity to sport-related trauma. The traumatic brain injury (TBI) is strongly associated with neurodegenerative pathology that affects cognition, memory and behavior. The current study developed a novel mild repetitive traumatic brain injury (mRTBI) model to highlight some of the possible molecular pathological mechanisms compared to those of single trauma. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00068993193018
Publisher Site
http://dx.doi.org/10.1016/j.brainres.2019.04.003DOI Listing
April 2019
1 Read

Prevalence and Molecular Epidemiology of Johne's Disease in Egyptian Cattle.

Acta Trop 2019 Apr 16. Epub 2019 Apr 16.

Department of Reproductive Diseases, Animal Reproduction Research Institute, ARC, Giza, Egypt.

Johne's disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actatropica.2019.04.019DOI Listing
April 2019
1 Read

Aggression: Perspectives from social and systems neuroscience.

Horm Behav 2019 Apr 16. Epub 2019 Apr 16.

Department of Biology, Bowdoin College, Brunswick, ME 04011, USA.

Exhibiting behavioral plasticity in order to mount appropriate responses to dynamic and novel social environments is crucial to the survival of all animals. Thus, how animals regulate flexibility in the timing, duration, and intensity of specific behaviors is of great interest to biologists. In this review, we discuss how animals rapidly respond to social challenges, with a particular focus on aggression. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yhbeh.2019.04.010DOI Listing
April 2019
1 Read

Dopamine drives binge-like consumption of a palatable food in experimental Parkinsonism.

Mov Disord 2019 Apr 19. Epub 2019 Apr 19.

Laboratorio di Neurofisiologia, IRCCS Fondazione Santa Lucia, Rome, Italy.

Background: Prolonged dopaminergic replacement therapy in PD results in pulsatile dopamine receptors stimulation in both dorsal and ventral striatum causing wearing off, motor fluctuations, and nonmotor side effects such as behavioral addictions. Among impulse control disorders, binge eating can be easily modeled in laboratory animals.

Objectives: We hypothesize that manipulation of dopamine levels in a 6-hydroxydopamine-lesioned rats, as a model of PD characterized by a different extent of dopamine denervation between dorsal and ventral striatum, would influence both synaptic plasticity of the nucleus accumbens and binge-like eating behavior. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/mds.27683DOI Listing

A recombinant bovine herpesvirus-4 vectored vaccine delivered via intranasal nebulization elicits viral neutralizing antibody titers in cattle.

PLoS One 2019 19;14(4):e0215605. Epub 2019 Apr 19.

Department of Veterinary Microbiology & Pathology, Washington State University, Pullman, Washington, United States of America.

Recombinant herpesvirus vaccine vectors offer distinct advantages in next-generation vaccine development, primarily due to the ability to establish persistent infections to provide sustainable antigen responses in the host. Recombinant bovine herpesvirus-4 (BoHV-4) has been previously shown to elicit protective immunity in model laboratory animal species against a variety of pathogens. For the first time, we describe the induction of antigen-specific immune responses to two delivered antigens in the host species after intranasal nebulization of recombinant BoHV-4 expressing the chimeric peptide containing the bovine viral diarrhea virus (BVDV) glycoprotein E2 and the bovine herpesvirus 1 (BoHV-1) glycoprotein D (BoHV-4-A-CMV-IgK-gE2gD-TM). Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215605PLOS

Simulation-based validation of spatial capture-recapture models: A case study using mountain lions.

PLoS One 2019 19;14(4):e0215458. Epub 2019 Apr 19.

Department of Ecology, Montana State University, Bozeman, Montana, United States of America.

Spatial capture-recapture (SCR) models have improved the ability to estimate densities of rare and elusive animals. However, SCR models have seldom been validated even as model formulations diversify and expand to incorporate new sampling methods and/or additional sources of information on model parameters. Information on the relationship between encounter probabilities, sources of additional information, and the reliability of density estimates, is rare but crucial to assessing reliability of SCR-based estimates. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215458PLOS
April 2019
1 Read

Adjunctive dabigatran therapy improves outcome of experimental left-sided Staphylococcus aureus endocarditis.

PLoS One 2019 19;14(4):e0215333. Epub 2019 Apr 19.

Department of Clinical Microbiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

Background: Staphylococcus aureus is the most frequent and fatal cause of left-sided infective endocarditis (IE). New treatment strategies are needed to improve the outcome. S. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215333PLOS

Inhibition of Tityus serrulatus venom hyaluronidase affects venom biodistribution.

PLoS Negl Trop Dis 2019 Apr 19;13(4):e0007048. Epub 2019 Apr 19.

Mestrado Profissional em Biotecnologia e Gestão da Inovação, Centro Universitário de Sete Lagoas, Sete Lagoas, Minas Gerais, Brazil.

Background: The hyaluronidase enzyme is generally known as a spreading factor in animal venoms. Although its activity has been demonstrated in several organisms, a deeper knowledge about hyaluronidase and the venom spreading process from the bite/sting site until its elimination from the victim's body is still in need. Herein, we further pursued the goal of demonstrating the effects of inhibition of T. Read More

View Article

Download full-text PDF

Source
http://dx.plos.org/10.1371/journal.pntd.0007048
Publisher Site
http://dx.doi.org/10.1371/journal.pntd.0007048DOI Listing
April 2019
1 Read

How host genetics dictates successful viral zoonosis.

PLoS Biol 2019 Apr 19;17(4):e3000217. Epub 2019 Apr 19.

BioFrontiers Institute and Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, United States of America.

Viruses of wild and domestic animals can infect humans in a process called zoonosis, and these events can give rise to explosive epidemics such as those caused by the HIV and Ebola viruses. While humans are constantly exposed to animal viruses, those that can successfully infect and transmit between humans are exceedingly rare. The key event in zoonosis is when an animal virus begins to replicate (one virion making many) in the first human subject. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pbio.3000217DOI Listing

Task-related hemodynamic responses are modulated by reward and task engagement.

PLoS Biol 2019 Apr 19;17(4):e3000080. Epub 2019 Apr 19.

Department of Neuroscience, Columbia University, New York, New York, United States of America.

Hemodynamic recordings from visual cortex contain powerful endogenous task-related responses that may reflect task-related arousal, or "task engagement" distinct from attention. We tested this hypothesis with hemodynamic measurements (intrinsic-signal optical imaging) from monkey primary visual cortex (V1) while the animals' engagement in a periodic fixation task over several hours was varied through reward size and as animals took breaks. With higher rewards, animals appeared more task-engaged; task-related responses were more temporally precise at the task period (approximately 10-20 seconds) and modestly stronger. Read More

View Article

Download full-text PDF

Source
http://dx.plos.org/10.1371/journal.pbio.3000080
Publisher Site
http://dx.doi.org/10.1371/journal.pbio.3000080DOI Listing
April 2019
1 Read

Role of Aqueous Extract of the Wood Ear Mushroom, Auricularia polytricha (Agaricomycetes), in Avoidance of Haloperidol-lnduced Catalepsy via Oxidative Stress in Rats.

Int J Med Mushrooms 2019 ;21(4):323-330

School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, 302017, Jaipur, India.

Haloperidol-induced catalepsy is an animal model of a psychotic disorder that may be associated with neurodegeneration and free radical damage. Auricularia polytricha is effective in both prevention and treatment of numerous types of neurological disorders. In the present study, anticataleptic activity of aqueous extract of A polytricha (AEAP) at different doses (400 and 600 mg/kg, respectively, p. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1615/IntJMedMushrooms.2019030351DOI Listing
January 2019
1 Read

Predicting Habitat Choice after Rapid Environmental Change.

Am Nat 2019 May 13;193(5):619-632. Epub 2019 Mar 13.

Decisions made while searching for settlement sites (e.g., nesting, oviposition) often have major fitness implications. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1086/702590DOI Listing

The circadian clock gene is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina.

FASEB J 2019 Apr 19:fj201801832RR. Epub 2019 Apr 19.

Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA.

A single pool of multipotent retinal progenitor cells give rise to the diverse cell types within the mammalian retina. Such cellular diversity is due to precise control of various cellular processes like cell specification, proliferation, differentiation, and maturation. Circadian clock genes can control the expression of key regulators of cell cycle progression and therefore can synchronize the cell cycle state of a heterogeneous population of cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801832RRDOI Listing

Genetic diversity and zoonotic potential of rotavirus A strains in the southern Andean highlands, Peru.

Transbound Emerg Dis 2019 Apr 19. Epub 2019 Apr 19.

Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil, 21.941-902.

Interspecies transmission is an important mechanism of evolution and contributes to RVA diversity. In order to evaluate the detection frequency, genetic diversity, epidemiological characteristics, and zoonotic potential of rotavirus A (RVA) strains in fecal specimens from humans and animals cohabiting in the same environment in the department of Cusco, Peru, by molecular analysis, 265 fecal specimens were obtained from alpacas, llamas, sheep, and shepherd children, and tested for RVA by RT-PCR. Genotyping was performed by multiplex PCR and sequence analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/tbed.13207DOI Listing

Alar fold resection in 25 horses: Clinical findings and effect on racing performance and airway mechanics (1998-2013).

Vet Surg 2019 Apr 19. Epub 2019 Apr 19.

Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.

Objective: To report clinical findings and performance in horses in which alar fold collapse (AFC) had been diagnosed and surgically treated and to assess form and degree of respiratory obstruction.

Study Design: Retrospective case series.

Animals: Twenty-one standardbreds, 2 coldblooded trotter racehorses, 1 thoroughbred, and 1 Icelandic horse. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/vsu.13214DOI Listing

Dietary baker's yeast sensitizes Ehrlich mammary adenocarcinoma to paclitaxel in mice bearing tumor.

Oncol Rep 2019 Apr 10. Epub 2019 Apr 10.

Department of Zoology, Faculty of Science, University of Mansoura, Mansoura 35516, Egypt.

Baker's yeast, Saccharomyces cerevisiae, has been shown to sensitize a variety of breast cancer cell (BCC) lines to paclitaxel chemotherapy in vitro. The present study evaluated the ability of S. cerevisiae to sensitize BCCs to paclitaxel in animals bearing Ehrlich ascites carcinoma (EAC). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/or.2019.7107DOI Listing

The use of aminoglycosides in animals within the EU: development of resistance in animals and possible impact on human and animal health: a review.

J Antimicrob Chemother 2019 Apr 19. Epub 2019 Apr 19.

Veterinary Medicines Directorate, Addlestone, UK.

Aminoglycosides (AGs) are important antibacterial agents for the treatment of various infections in humans and animals. Following extensive use of AGs in humans, food-producing animals and companion animals, acquired resistance among human and animal pathogens and commensal bacteria has emerged. Acquired resistance occurs through several mechanisms, but enzymatic inactivation of AGs is the most common one. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/jac/dkz161DOI Listing

How do richness and composition of diet shape trace metal exposure in a free-living generalist rodent, Apodemus sylvaticus.

Environ Sci Technol 2019 Apr 19. Epub 2019 Apr 19.

Exposure of terrestrial mammals to chemical contaminants like trace metals (TMs) is considered to be mainly based on trophic transfer. Although relationships between TM transfer to animals and identity of contaminated food have been studied, the variation of the TM transfer with respect to diet diversity has been poorly documented. In this study, the oral exposure to TMs of wood mice Apodemus sylvaticus was investigated with respect to both the number of different items, i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.8b07194DOI Listing

Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe.

ACS Sens 2019 Apr 19. Epub 2019 Apr 19.

Oxygenation and tissue hypoxia play critical roles in mammalian biology and contribute to aggressive phenotypes in cancerous tumors, driving research to develop accurate and easy to implement methods for monitoring hypoxia in living cells and animal models. This study reports the chemiluminescent probe HyCL-4-AM, which contains a nitroaromatic sensing moiety and, importantly, an acetoxymethyl (AM) ester that dramatically improves operation in cells and animals. HyCL-4-AM provides a selective 60,000-fold increase in luminescence emission in the presence of rat liver microsomes (RLM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acssensors.9b00360DOI Listing

TRPC channels are not required for graded persistent activity in entorhinal cortex neurons.

Hippocampus 2019 Apr 19. Epub 2019 Apr 19.

Institute of Physiology and Pathophysiology, Heidelberg University, Heidelberg, Germany.

Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/hipo.23094DOI Listing

GLUT12 and adipose tissue: Expression, regulation and its relation with obesity in mice.

Acta Physiol (Oxf) 2019 Apr 19:e13283. Epub 2019 Apr 19.

University of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain.

Aim: The facilitative glucose transporter GLUT12 was isolated from the breast cancer cell line MCF-7 by its homology with GLUT4. GLUT12 is expressed in insulin sensitive tissues such as adipose tissue. The aim of this work was to investigate GLUT12 expression and hormonal regulation in 3T3-L1 adipocytes and in adipose tissue of lean and diet-induced obese mice. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.13283
Publisher Site
http://dx.doi.org/10.1111/apha.13283DOI Listing
April 2019
1 Read

Development and validation of a loop-mediated isothermal amplification (LAMP) assay - a rapid and sensitive detection tool for Mycobacterium avium subsp. paratuberculosis in small ruminants.

J Appl Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation Bünteweg 17, 30559, Hannover, Germany.

Aims: The aim of this study was to design an assay for the identification of Mycobacterium avium subsp. paratuberculosis (MAP) to be used in feces and milk samples of small ruminants with a loop-mediated isothermal amplification (LAMP) system, as a time saving and user-friendly method in contrast to real-time PCR.

Methods And Results: For the detection of MAP in milk and feces of small ruminants, we developed a set of primers, specific for the target gene ISMap02. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.14284
Publisher Site
http://dx.doi.org/10.1111/jam.14284DOI Listing
April 2019
1 Read

Micro- and macroparasite species richness in birds: the role of host life history and ecology.

J Anim Ecol 2019 Apr 19. Epub 2019 Apr 19.

NIOZ Royal Netherlands Institute for Sea Research, Department of Coastal Systems, and Utrecht University, P.O. Box 59, 1790 AB, Den Burg, Texel, Netherlands.

1.Identifying the factors shaping variation in parasite diversity among host species is crucial to understand wildlife diseases. Although micro- and macroparasites may exert different selective pressures on their hosts, studies investigating the determinants of parasite species richness in animals have rarely considered this divide. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.12
Publisher Site
http://dx.doi.org/10.1111/1365-2656.12998DOI Listing
April 2019
1 Read

Knockout of apolipoprotein A-I decreases parenchymal and vascular β-amyloid pathology in the Tg2576 mouse model of Alzheimer's disease.

Neuropathol Appl Neurobiol 2019 Apr 19. Epub 2019 Apr 19.

School of Life, Health and Chemical Sciences, STEM Faculty, The Open University, Milton Keynes, UK.

Aims: Apolipoprotein A-I (apoA-I), the principal apolipoprotein associated with high density lipoproteins (HDL) in the periphery, is also found at high concentrations in the cerebrospinal fluid. Previous studies have reported either no impact or vascular-specific effects of apoA-I knockout on β-amyloid (Aβ) pathology. However, the putative mechanism(s) by which apoA-I may influence Aβ deposition is unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/nan.12556DOI Listing

The effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injury.

Eur Rev Med Pharmacol Sci 2019 Apr;23(7):3088-3095

Department of Surgery, Istinye University, Faculty of Medicine, VM Mersin Medical Park Hospital, Mersin, Turkey.

Objective: Mammalian transient receptor potential melastatin (TRPM) channels are a form of calcium channels and they transport calcium and magnesium ions. TRPM has eight subclasses including TRPM1-8. TRPM2, TRPM6, TRPM7, TRPM8 are expressed especially in the liver cell. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.26355/eurrev_201904_17592DOI Listing

Feeding Behavior of Mangalarga Marchador Weanlings Fed Sorghum Silage Versus Grass Hay.

J Equine Vet Sci 2019 Apr 13;75:90-92. Epub 2019 Feb 13.

Departamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

Mangalarga Marchador foals in Minas Gerais, Brazil, are usually weaned during the dry season, when there is a scarcity of forage. Although sorghum silage has been used to feed weanlings, the effect on their feeding behavior in comparison with grass hay has not been reported. Twelve Mangalarga Marchador weanlings were randomly assigned to two groups of six animals: SS: sorghum silage; VH: Vaquero hay. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jevs.2019.02.001DOI Listing

Effect of Topical 1% Cyclopentolate Hydrochloride on Tear Production, Intraocular Pressure, and Pupil Size in Healthy Turkman Horses.

J Equine Vet Sci 2019 Apr 29;75:25-29. Epub 2019 Jan 29.

Member of Turkmen Horse Breeding & Consulting Co. (THBC), Jargalan, North Khorasan Province, Iran.

This study was performed to evaluate the effect of topical 1% cyclopentolate hydrochloride on tear production (STT), intraocular pressure (IOP), and vertical pupil diameter (VPD) in healthy Turkman horses. Forty eyes of 20 clinically normal horses were used for this study, which were randomly assigned to two groups. Before instillation of 1% cyclopentolate, the baseline STT, IOP, and VPD of each group were recorded. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jevs.2019.01.002DOI Listing

Interobserver Reliability of the Animal Welfare Indicators Welfare Assessment Protocol for Horses.

J Equine Vet Sci 2019 Apr 14;75:112-121. Epub 2019 Feb 14.

Institute of Animal Breeding and Husbandry, Kiel University, Kiel, Germany.

Objective tools for the assessment of animal welfare are needed. The present study analyzed the interobserver reliability of the Animal Welfare Indicators (AWIN) welfare assessment protocol for horses to further enhance knowledge concerning reliability. Therefore, two trained observers conducted 18 assessments on farm at the same time and on the same animals. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S07370806183070
Publisher Site
http://dx.doi.org/10.1016/j.jevs.2019.02.005DOI Listing
April 2019
1 Read

Lassa Virus Targeting of Anterior Uvea and Endothelium of Cornea and Conjunctiva in Eye of Guinea Pig Model.

Emerg Infect Dis 2019 May;25(5):865-874

Lassa virus (LASV), a hemorrhagic fever virus endemic to West Africa, causes conjunctivitis in patients with acute disease. To examine ocular manifestations of LASV, we histologically examined eyes from infected guinea pigs. In fatal disease, LASV immunostaining was most prominent in the anterior uvea, especially in the filtration angle, ciliary body, and iris and in and around vessels in the bulbar conjunctiva and peripheral cornea, where it co-localized with an endothelial marker (platelet endothelial cell adhesion molecule). Read More

View Article

Download full-text PDF

Source
http://wwwnc.cdc.gov/eid/article/25/5/18-1254_article.htm
Publisher Site
http://dx.doi.org/10.3201/eid2505.181254DOI Listing
May 2019
1 Read

In Vivo Immunological Effects of CD73 Deficiency.

Cell Physiol Biochem 2019 ;52(5):1192-1202

Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany.

Background/aims: The extracellular ecto-5'-nucleotidase (CD73) is involved in the production of immunosuppressive adenosin (Ado), which can influence different immune cells through the specific adenosine receptors. The main aim of this work was to characterize immune cell populations as well as serum cytokine level in healthy CD73-deficient mice compared to healthy wild-type animals.

Methods: Profound immnophenotyping of splenocytes from healthy CD73-deficient and wild-type mice was done using flow cytometry (FACS analysis). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000081DOI Listing
January 2019
2 Reads

[Advances in isopeptide bond-mediated molecular superglue].

Sheng Wu Gong Cheng Xue Bao 2019 Apr;35(4):607-615

College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.

Isopeptide bond-mediated molecular superglue is the irreversible covalent bond spontaneously formed by the side chains of lysine (Lys) and asparagine/aspartic acid (Asn/Asp) residues. The peptide-peptide interaction is specific, stable, and can be achieved quickly without any particular physicochemical factor. In the light of recent progress by domestic and foreign researchers, here we summarize the origin, assembly system and mechanism of isopeptide bond reaction, as well as the molecular cyclization and protein topological structure mediated by it. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.13345/j.cjb.180303DOI Listing

Esophageal feeding tube placement and the associated complications in 248 cats.

J Vet Intern Med 2019 Apr 18. Epub 2019 Apr 18.

Department of Internal Medicine, Royal (Dick) School of Veterinary Studies, Ringgold Standard Institution-Internal Medicine, University of Edinburgh, Easter Bush, United Kingdom.

Background: Esophageal feeding tubes are commonly used to provide enteral nutrition to cats, but their use is associated with adverse effects.

Objectives: To evaluate the complications associated with e-tube placement in cats and to identify factors predisposing to these complications.

Animals: Cats that had an esophageal feeding tube placed (n = 248). Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/jvim.15496
Publisher Site
http://dx.doi.org/10.1111/jvim.15496DOI Listing
April 2019
1 Read

2-Hydroxypyridine-N-Oxide (HOPO) is Not Genotoxic In Vivo.

Environ Mol Mutagen 2019 Apr 18. Epub 2019 Apr 18.

Pfizer Worldwide Research and Development, Drug Safety, Genetic Toxicology Center of Emphasis, Groton, CT 06340.

2-Hydroxypyridine N-oxide (HOPO) is an important coupling reagent used in pharmaceutical synthesis. Our laboratory previously reported HOPO as equivocal in the Ames assay following extensive testing of multiple lots of material. Given the lack of reproducibility between lots of material, and the weak increase in revertants observed, it was concluded that it would be highly unlikely that HOPO would pose a mutagenic risk in vivo. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/em.22294
Publisher Site
http://dx.doi.org/10.1002/em.22294DOI Listing
April 2019
1 Read

Cholecalciferol (Vitamin D) Reduces Rat Neuropathic Pain by Modulating Opioid Signaling.

Mol Neurobiol 2019 Apr 18. Epub 2019 Apr 18.

Institut de NeuroPhysioPathologie, CNRS UMR 7051, Aix-Marseille Université - Faculté de Médecine Nord, CS 811. 51 Bd P. Dramard, 13344, Marseille Cedex 15, France.

The impact of vitamin D on sensory function, including pain processing, has been receiving increasing attention. Indeed, vitamin D deficiency is associated with various chronic pain conditions, and several lines of evidence indicate that vitamin D supplementation may trigger pain relief. However, the underlying mechanisms of action remain poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-019-1582-6DOI Listing

Characterization of earthworm α-amylases for dietary supplement development and biomass utilization.

Environ Sci Pollut Res Int 2019 Apr 18. Epub 2019 Apr 18.

Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.

Earthworms are useful soil-decomposing animals that possess various saccharification enzymes such as cellulases and amylases. Earthworms have also been traditionally used as antipyretic agents and medicines for preventing thrombotic diseases such as brain infarction. We previously developed a novel earthworm dietary supplement with fibrinolytic, cellulase, and amylase activities using high-pressure technology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-019-05133-xDOI Listing

Behavioral and biochemical effects of the antifouler and antidandruff zinc pyrithione on the freshwater fish Gambusia holbrooki.

Fish Physiol Biochem 2019 Apr 18. Epub 2019 Apr 18.

Departamento de Biologia/CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.

The presence of pharmaceutical residues in the aquatic environment is receiving great attention since the levels of these substances have significantly increased in this compartment, potentially leading to adverse ecological effects. Zinc pyrithione (ZnPt) is a widely used organometallic biocide, which is incorporated into antifouling formulas, such as paints, to prevent the establishment of biofilms on surfaces exposed to the aquatic environment. It is also used in cosmetics, such as antidandruff shampoos and soaps. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s10695-019-00634-5
Publisher Site
http://dx.doi.org/10.1007/s10695-019-00634-5DOI Listing
April 2019
2 Reads

Safety assessment of the pharmacological excipient, diethylene glycol monoethyl ether (DEGEE), using in vitro and in vivo systems.

Daru 2019 Apr 18. Epub 2019 Apr 18.

Division of Toxicology and Experimental Medicine, CSIR- Central Drug Research Institute, Lucknow, Uttar Pradesh, 226031, India.

Background: Diethylene glycol monoethyl ether (DEGEE) is widely used as a solubilizer in cosmetics as well as in oral, topical, transdermal and injectable pharmaceutical formulations. Due to the unavailability of detailed toxicological studies on DEGEE, the Scientific Committee on Consumer Products (SCCP) found its toxicological reports to be unsatisfactory, comprising only summaries. Also, a few reports have raised concern on the use of DEGEE as it might cause damage to the kidneys. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40199-019-00264-5DOI Listing

Urine salts elucidate Early Neolithic animal management at Aşıklı Höyük, Turkey.

Sci Adv 2019 Apr 17;5(4):eaaw0038. Epub 2019 Apr 17.

Department of Prehistory, Istanbul University, Istanbul 34134, Turkey.

The process of sheep and goat (caprine) domestication began by 9000 to 8000 BCE in Southwest Asia. The early Neolithic site at Aşıklı Höyük in central Turkey preserves early archaeological evidence of this transformation, such as culling by age and sex and use of enclosures inside the settlement. People's strategies for managing caprines evolved at this site over a period of 1000 years, but changes in the scale of the practices are difficult to measure. Read More

View Article

Download full-text PDF

Source
http://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aaw
Publisher Site
http://dx.doi.org/10.1126/sciadv.aaw0038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469938PMC
April 2019
1 Read

Short-, Mid-, and Long-Term Effect of Granulocyte Colony-Stimulating Factor/Stem Cell Factor and Fms-Related Tyrosine Kinase 3 Ligand Evaluated in an In Vivo Model of Hypoxic-Hyperoxic Ischemic Neonatal Brain Injury.

Biomed Res Int 2019 13;2019:5935279. Epub 2019 Mar 13.

Department of Pediatrics II (Neonatology), Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.

Hematopoietic growth factors are considered to bear neuroprotective potential. We have previously shown that delayed treatment with granulocyte colony-stimulating factor (G-CSF)/stem cell factor (SCF) and Fms-related tyrosine kinase 3 ligand (FL) ameliorates excitotoxic neonatal brain injury. The effect of these substances in combined-stressor neonatal brain injury models more closely mimicking clinical conditions has not been investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/5935279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436372PMC

Congenital Transmission of After Experimental Reinfection With Brazilian Typical Strains in Chronically Infected Sheep.

Front Vet Sci 2019 2;6:93. Epub 2019 Apr 2.

Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo, São Paulo, Brazil.

is a cause of congenital diseases, miscarriages and stillbirths in production animals. In Brazil, non-archetypal genotypes of the parasite may be related to severe disease. Experimental infection with was studied in sheep to analyse congenital transmission-related parameters in reinfections with different Brazilian parasite strains. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2019.00093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454189PMC
  • Page 1 of 129557