Search Our Scientific Publications & Authors

J Am Assoc Lab Anim Sci 2022 Jun 24. Epub 2022 Jun 24.

Charles River Laboratories, Research Models and Services, Wilmington, Massachusetts.

Molecular-based methods have shown potential for improving pathogen detection and reducing animal use. While increasing evidence supports rodent-free environmental health PCR pathogen detection, limited information is available regarding efficacy for disposable individually ventilated caging systems. In such systems, testing of plenum exhaust air dust is ineffective, and the use of collection media is optimal. Read More

Air Med J 2022 Jul-Aug;41(4):411-412. Epub 2022 Apr 11.

Life Flight, Memorial Hermann Hospital-Texas Medical Center, Houston, TX; Department of Surgery, University of Texas Health Science Center at Houston, Houston, TX.

This short communication highlights the development and implementation of the first civilian helicopter air ambulance canine response program in the United States. Read More

Chin J Nat Med 2022 Jun;20(6):458-472

School of Traditional Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. It is known that aucubin (AU) exerts anti-inflammatory activity, but its effects and mechanisms in RA are unclear. This study investigated the anti-inflammatory effects and mechanisms of AU in vivo and in vitro. Read More

Int Rev Neurobiol 2022 18;163:285-310. Epub 2022 Mar 18.

Icahn School of Medicine at Mount Sinai, New York City, NY, United States. Electronic address:

Although essential tremor is common, its underlying pathophysiology remains uncertain, and several hypotheses seek to explain the tremor mechanism. The GABA hypothesis states that disinhibition of deep cerebellar neurons due to reduced GABAergic input from Purkinje cells results in increased pacemaker activity, leading to rhythmic output to the thalamo-cortical circuit and resulting in tremor. However, some neuroimaging, spectroscopy, and pathology studies have not shown a clear or consistent GABA deficiency in essential tremor, and animal models have indicated that large reductions of Purkinje cell inhibition may improve tremor. Read More

Int Rev Neurobiol 2022 21;163:259-284. Epub 2022 Mar 21.

Movement Disorders Unit, Department of Neurology, Sant Pau Hospital, Autonomous University of Barcelona, Catalonia, Spain. Electronic address:

Dysfunction in gamma-aminobutyric acid (GABA) neurotransmission has emerged as a prime suspect for the underlying neurochemical dysfunction in essential tremor (ET). This dysfunction has been termed the GABA hypothesis. We review findings to date supporting the 4 steps in this hypothesis in studies of cerebrospinal fluid, pathology, genetics, animal models, imaging, computational models, and human drugs, while not overlooking the evidence of negative studies and controversies. Read More

J Immunol 2022 Jun 24. Epub 2022 Jun 24.

Department of Neurology, The University of North Carolina at Chapel Hill, Chapel Hill, NC;

The objective of this study is to determine the mechanism of action of anti-CD52 mAb treatment in patients with relapsing-remitting multiple sclerosis (RRMS). Experimental autoimmune encephalomyelitis (EAE), an animal model of the disease, was used to address the role of T regulatory cells (Tregs) in the anti-CD52 mAb-induced suppression of the disease. In vitro studies on PBMCs from RRMS patients and matched healthy controls determined the effect of IL-7 on the expansion of CD4CD25CD127 Tregs and induction of their suppressive phenotype. Read More

Environ Pollut 2022 Jun 21:119658. Epub 2022 Jun 21.

College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shannxi, 712100, China. Electronic address:

Biological invasions and continued salinization of freshwater are two global issues with largely serious ecological consequences. Increasing salinity in freshwater systems, as an environmental stressor, may negatively affect normal life activities in fish. It has been documented that salinity limits the invasive success of alien species by mediating physiological and life-history performances, however, there are few studies on how salinity affects its invasive process via altered behaviors. Read More

Transl Res 2022 Jun 21. Epub 2022 Jun 21.

Nephrology Division, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute (HSCI), Cambridge, MA, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. Electronic address:

The kidney is a vital organ that regulates the bodily fluid and electrolyte homeostasis via tailored urinary excretion. Kidney injuries that cause severe or progressive chronic kidney disease have driven the growing population of patients with end-stage kidney disease, leading to substantial patient morbidity and mortality. This irreversible kidney damage has also created a huge socioeconomical burden on the healthcare system, highlighting the need for novel translational research models for progressive kidney diseases. Read More

Metabolism 2022 Jun 21:155242. Epub 2022 Jun 21.

Dept of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy. Electronic address:

Introduction And Methods: Skeletal muscle mitochondrial dysfunction may cause tissue oxidative stress and consequent catabolism in chronic kidney disease (CKD), contributing to patient mortality. We investigated in 5/6-nephrectomized (Nx) rats the impact of n3-polyunsaturated fatty-acids (n3-PUFA) isocaloric partial dietary replacement on gastrocnemius muscle (Gm) mitochondrial master-regulators, ATP production, ROS generation and related muscle-catabolic derangements.

Results: Nx had low Gm mitochondrial nuclear respiratory factor-2 and peroxisome proliferator-activated receptor gamma coactivator-1alpha, low ATP production and higher mitochondrial fission-fusion protein ratio with ROS overproduction. Read More

Chemosphere 2022 Jun 21:135449. Epub 2022 Jun 21.

UCIBIO-REQUIMTE - Applied Molecular Biosciences Unit, Department of Drug Sciences, Pharmacology Lab, Faculty of Pharmacy, University of Porto, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy, Department of Drug Sciences, Pharmacology Lab, Faculty of Pharmacy, University of Porto, Portugal. Electronic address:

Behavioural disruptions are sensitive indicators of alterations to normal animal physiology and can be used for toxicity assessment. The small vertebrate zebrafish is a leading model organism for toxicological studies. The ability to continuously monitor the toxicity of drugs, pollutants, or environmental changes over several days in zebrafish can have high practical application. Read More

Life Sci 2022 Jun 21:120726. Epub 2022 Jun 21.

Institute of Neuroanatomy, Uniklinik RWTH Aachen, Aachen, Germany. Electronic address:

Aims: Spinal cord injury (SCI) is a debilitating neurological condition often associated with chronic neuroinflammation and redox imbalance. Oxidative stress is one of the main hallmark of secondary injury of SCI which is tightly regulated by nuclear factor E2-related factor 2/antioxidant response element (Nrf2/ARE) signaling. In this study, we aimed at investigating the interplay between inflammation-related miRNAs and the Nrf2 pathway in animal model of SCI. Read More

Neurobiol Dis 2022 Jun 21:105798. Epub 2022 Jun 21.

Department of Neurology, University Hospital of Würzburg, Josef-Schneider-Straße 11, 97080 Würzburg, Germany. Electronic address:

The pathogenesis of Parkinson's disease (PD) is closely interwoven with the process of aging. Moreover, increasing evidence from human postmortem studies and from animal models for PD point towards inflammation as an additional factor in disease development. We here assessed the impact of aging and inflammation on dopaminergic neurodegeneration in the hmα-SYN-39 mouse model of PD that carries the human, A30P/A53T double-mutated α-synuclein gene. Read More

Virus Res 2022 Jun 21:198850. Epub 2022 Jun 21.

Multiuser Biomolecular Innovation Laboratory, Department of Physics, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University "Júlio de Mesquita Filho" (UNESP), São José do Rio Preto, SP, Brazil. Electronic address:

The human Respiratory Syncytial Virus (hRSV) is the main causative agent of acute respiratory infections (ARI), such as pneumonia and bronchiolitis. One of the factors that lead to success in viral replication is the interaction of the M2-2 protein with the ribosomal complex. This interaction is responsible for the phase change of viral activity, acting as an inhibitor or inducer of viral replication, according to the concentration of mRNA. Read More

Fish Shellfish Immunol 2022 Jun 21. Epub 2022 Jun 21.

College of Marine Sciences, South China Agricultural University, Guangzhou, China; Guangdong Province Engineering Research Centre of Aquatic Immunization and Aquaculture Health Techniques, South China Agricultural University, Guangzhou, China; University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, China. Electronic address:

Aeromonas hydrophila was a common bacterial pathogen in aquaculture resulting in considerable losses to the striped catfish aquaculture industry. As an emergent antimicrobial peptide (AMP), NK-lysin (NKL) had activity against various microorganisms. However, the antibacterial activity of NKL from striped catfish (Pangasianodon hypophthalmus) both in vitro and vivo remains unclear. Read More

J Ethnopharmacol 2022 Jun 21:115496. Epub 2022 Jun 21.

Hospital of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, 210042, China. Electronic address:

Ethnopharmacological Relevance: Tripterygium wilfordii polyglycosides (TWP) are extracted from Tripterygium wilfordii Hook. f., which has the significant effects of anti-inflammation and immunosuppression and has been widely used to treat autoimmune diseases in traditional Chinese medicine. Read More

Biol Psychol 2022 Jun 21:108383. Epub 2022 Jun 21.

Southern Methodist University, Dallas, Texas, USA. Electronic address:

Food Chem Toxicol 2022 Jun 21:113251. Epub 2022 Jun 21.

Centro de Química Estrutural, Institute of Molecular Sciences, Departamento de Engenharia Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049 001, Lisboa, Portugal. Electronic address:

Acrylamide and furan are environmental and food contaminants that are metabolized by cytochrome P450 2E1 (CYP2E1), giving rise to glycidamide and cis-2-butene-1,4-dial (BDA) metabolites, respectively. Both glycidamide and BDA are electrophilic species that react with nucleophilic groups, being able to introduce mutations in DNA and perform epigenetic remodeling. However, whereas these carcinogens are primarily metabolized in the liver, the carcinogenic potential of acrylamide and furan in this organ is still controversial, based on findings from experimental animal studies. Read More

Lancet Microbe 2022 Jun 21. Epub 2022 Jun 21.

Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA. Electronic address:

Background: The emergence of increasingly antimicrobial-resistant Salmonella enterica serovar Typhi (S Typhi) threatens to undermine effective treatment and control. Understanding where antimicrobial resistance in S Typhi is emerging and spreading is crucial towards formulating effective control strategies.

Methods: In this genomic epidemiology study, we sequenced the genomes of 3489 S Typhi strains isolated from prospective enteric fever surveillance studies in Nepal, Bangladesh, Pakistan, and India (between 2014 and 2019), and combined these with a global collection of 4169 S Typhi genome sequences isolated between 1905 and 2018 to investigate the temporal and geographical patterns of emergence and spread of antimicrobial-resistant S Typhi. Read More

Lancet Microbe 2022 Jun 21. Epub 2022 Jun 21.

Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; National Avian Influenza Para-reference Laboratory, Guangzhou, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, National Development and Reform Commission of the People's Republic of China, Guangzhou, China; Key Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Guangzhou, China; Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, Guangzhou, China. Electronic address:

Immunity 2022 Jun 8. Epub 2022 Jun 8.

Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3000, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, VIC 3000, Australia; Department of General Medicine, Royal Children's Hospital Melbourne, Melbourne, VIC 3000, Australia.

As the establishment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell memory in children remains largely unexplored, we recruited convalescent COVID-19 children and adults to define their circulating memory SARS-CoV-2-specific CD4 and CD8 T cells prior to vaccination. We analyzed epitope-specific T cells directly ex vivo using seven HLA class I and class II tetramers presenting SARS-CoV-2 epitopes, together with Spike-specific B cells. Unvaccinated children who seroconverted had comparable Spike-specific but lower ORF1a- and N-specific memory T cell responses compared with adults. Read More

Domest Anim Endocrinol 2022 Jun 2;81:106746. Epub 2022 Jun 2.

UMR Physiologie de la Reproduction et des Comportements, INRAE, CNRS, IFCE, Université de Tours, Agreenium, 37380 Nouzilly, France.

Male goats rendered sexually active by exposure to a photoperiodic treatment are more efficient than untreated goats in stimulating LH secretion and ovulation in seasonally anestrous goats. This phenomenon is called the "male effect." Here, we determined whether sexually active bucks are able to stimulate the endocrine and sexual activities of other bucks in seasonal sexual rest through the phenomenon that we called the "buck-to-buck effect. Read More

Phys Med 2022 Jun 21;100:64-71. Epub 2022 Jun 21.

Institut Curie, Université Paris-Saclay, CNRS, UMR3347, Inserm U1021, Signalisation Radiobiologie et Cancer, 91400 Orsay, France. Electronic address:

Proton minibeam radiation therapy (pMBRT) is a novel therapeutic strategy that combines the normal tissue sparing of sub-millimetric, spatially fractionated beams with the improved ballistics of protons. This may allow a safe dose escalation in the tumour and has already proven to provide a remarkable increase of the therapeutic index for high-grade gliomas in animal experiments. One of the main challenges in pMBRT concerns the generation of minibeams and the implementation in a clinical environment. Read More

Environ Int 2022 Jun 18;166:107362. Epub 2022 Jun 18.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

The adverse effects of plastic on adult animal and human health have been receiving increasing attention. However, its potential toxicity to fetuses has not been fully elucidated. Herein, biodistribution of polystyrene (PS) particles was determined after the maternal mice were orally given PS micro- and/or nano-particles with and without surface modifications during gestational days 1 to 17. Read More

Anim Reprod Sci 2022 Jun 16;243:107025. Epub 2022 Jun 16.

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Egypt.

Pikeperch is one of the major new fish species in freshwater aquaculture. Artificial reproduction is difficult in this species, and many aspects require further study. These include synchronization of female maturation, latency time, gamete quality and spawner survival rate. Read More

Talanta 2022 Jun 20;249:123706. Epub 2022 Jun 20.

Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China. Electronic address:

In this paper, a portable fluorescence-based functional hydrogel loaded with β-d-glucose pentaacetate (β-D-GP) is designed for high-sensitive quantification of amine vapor and visual monitoring of freshness of shrimp. We found for the first time that amine vapor can mediate β-D-GP to generate photoluminescent polymer-carbon nanodots (PCNDs) with good optical properties. On this basis, a functional hydrogel sensing platform is simply formed by solidifying β-D-GP in agarose hydrogels. Read More

J Hazard Mater 2022 Jun 2;437:129266. Epub 2022 Jun 2.

Department of Civil and Environmental Engineering, University of California, Irvine, Irvine, CA 92697-2175, USA. Electronic address:

Aquaculture generates significant amount of processing wastes (more than 500 million pounds annually in the United States), the bulk of which ends up in the environment or is used in animal feed. Proper utilization of shrimp waste can increase their economic value and divert them from landfills. In this study, shrimp waste was converted to a porous carbon (named SPC) via direct pyrolysis and activation. Read More

Food Chem 2022 Jun 18;394:133517. Epub 2022 Jun 18.

Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address:

Malachite green (MG) is an organic dye compound that is frequently used as a fungicide and antiseptic in aquaculture. However, human or animal exposure to MG causes carcinogenic, teratogenic and mutagenic effects. Herein, a novel fluorescent assay was designed for the detection of MG using manganese dioxide nanosheets (MnO NS) as an energy acceptor to quench the fluorescence of branched poly(ethylenimine) carbon dots (BPEI-CDs) via Förster resonance energy transfer. Read More

Vet Parasitol 2022 Jun 13;309:109756. Epub 2022 Jun 13.

Brazilian Agricultural Research Corporation (EMBRAPA), Southeast Livestock, Rodovia Washington Luiz, Km 234s/n, Fazenda Canchim, PO Box 339, 13560-970, São Carlos, SP, Brazil. Electronic address:

Nanocarriers of acaricidal compounds improve the bioavailability, absorption, and tissue distribution of active ingredients, releasing them in a slow, targeted way and protecting them against premature degradation. Thus, this study aimed to develop formulations from solid lipid nanoparticles (SLN), or nanostructured lipid carriers (NLC) associated with cypermethrin (cip) + chlorpyrifos (chlo) and vegetable compounds (citral, menthol, or limonene). Particles were then characterised, and their efficacy was verified on R. Read More

Phytomedicine 2022 Jun 15;104:154241. Epub 2022 Jun 15.

Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Background: Non-alcoholic steatohepatitis (NASH) has replaced viral hepatitis as the main driver of the rising morbidity and mortality associated with cirrhosis and liver cancer worldwide, while no FDA-approved therapies are currently known. Kinsenoside (KD), naturally isolated from Anoectochilus roxburghii, possesses multiple biological activities, including lipolysis, anti-inflammation, and hepatoprotection. However, the effects of KD on NASH remain unclear. Read More

Anim Biotechnol 2022 Jun 24:1-7. Epub 2022 Jun 24.

Department of Poultry Production, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.

The present study was conducted to interpret the effect of magnetic field on water pH and calcium form, and evaluate semen quality, fertility and hatchability rates of Fayoumi chickens receiving magnetized drinking water (MDW). A total of 180 Fayoumi chickens (20 roosters + 160 hens), 54 weeks of age, were divided into two groups. The first group served as control, which received regular drinking water, and the second group was treated with MDW. Read More