4,705 results match your criteria Animal Reproduction Science[Journal]


Chemosynthetic ethanolamine plasmalogen stimulates gonadotropin secretion from bovine gonadotrophs by acting as a potential GPR61 agonist.

Anim Reprod Sci 2022 May 13;241:106992. Epub 2022 May 13.

Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Transborder Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.

Brain ethanolamine plasmalogens (EPls) are unique alkenylacyl-glycerophospholipids and the only recognized ligands of G-protein-coupled receptor 61 (GPR61), a newly identified receptor that colocalizes with GnRH receptors on gonadotrophs. As the chemical synthesis of EPl is challenging, only one chemosynthetic EPl, 1-(1Z-octadecenyl)- 2-oleoyl-sn-glycero-3-phosphoethanolamine (PLAPE; C18:0-C18:1), is commercially available. Therefore, we tested the hypothesis that PLAPE stimulates gonadotropin secretion from bovine gonadotrophs. Read More

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Effect of roscovitine pretreatment for increased utilization of small follicle-derived oocytes on developmental competence of somatic cell nuclear transfer embryos in pigs.

Anim Reprod Sci 2022 May 4;241:106987. Epub 2022 May 4.

Department of Animal Cloning, Institute of Animal Genetic Technology, Branch of Biotechnology, State Academy of Sciences, Pyongyang, the Democratic People's Republic of Korea; College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

The objective of this study was to evaluate the effect of roscovitine pretreatment on the number of matured oocytes per ovary available for somatic cell nuclear transfer (SCNT) and their developmental competence. Irrespective of reproduction status (prepuberty/puberty), the average number of small follicles per ovary (19.3/17. Read More

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Motility and oxidative stress of common carp Cyprinus carpio sperm during short-term storage.

Anim Reprod Sci 2022 May 10;241:106991. Epub 2022 May 10.

CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.

Short-term storage of semen is a simple and inexpensive procedure to deal with logistics of large-scale hatchery operations but can lead to oxidative stress and a significant decrease in sperm motility and velocity. To better understand the mechanisms responsible for the association of poor sperm quality with oxidative stress, in the present study we investigated the effect of refrigerated storage for 0, 24, 48, 72, and 144 h on sperm motility and curvilinear velocity and oxidant/antioxidant balance of common carp Cyprinus carpio. Percentage of motile sperm was significantly (p < 0. Read More

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The influence of prorenin/(pro)renin receptor on progesterone secretion by the bovine corpus luteum.

Anim Reprod Sci 2022 Apr 29;241:106985. Epub 2022 Apr 29.

Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, RS, Brazil. Electronic address:

The aim of this study was to evaluate the role of prorenin/(pro)renin receptor activation on luteal progesterone (P4) secretion. Our hypothesis was that the nonproteolytic activation of (pro)renin receptor [P(RR)] is part of the regulatory mechanism responsible for corpus luteum (CL) function. In the first three experiments, prorenin was found to stimulate the production of P4, which is not inhibited by an angiotensin receptor antagonist (saralasin), but rather by a renin/prorenin inhibitor (aliskiren), a MAPK1/3 inhibitor (PD325901) or an EGFR inhibitor (AG1478), which are evidence of nonproteolytic activation of prorenin. Read More

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TBBPA downregulates thyroid receptor and estrogen receptor mRNA levels in goldfish gonadal tissue.

Anim Reprod Sci 2022 May 6;240:106990. Epub 2022 May 6.

Department of Microbiology and Immunology, School of Medicine, University of Nevada, Reno, NV, USA. Electronic address:

Tetrabromobisphenol A (TBBPA) is a brominated flame retardant that has been shown to be a potential thyroid disrupting chemical. Currently, TBBPA is not included in the UN's list of endocrine disruptors and adverse effects of TBBPA in mammals has not been fully investigated. However, there is clear evidence that TBBPA exerts adverse health effects on reproduction of aquatic species. Read More

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Expression patterns of genes in steroidogenic, cholesterol uptake, and liver x receptor-mediated cholesterol efflux pathway regulating cholesterol homeostasis in natural and PGF2α induced luteolysis as well as early pregnancy in ovine corpus luteum.

Anim Reprod Sci 2022 May 5;240:106988. Epub 2022 May 5.

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey.

The aim was to evaluate the expression of genes of steroidogenic, cholesterol uptake, and liver X receptor (LXR) mediated cholesterol efflux pathway in ovine corpus luteum (CL) during natural and prostaglandin F2α (PGF2α) induced luteolysis and early pregnancy. For this study, two experiments were carried out 1); ewes were grouped into two sub-groups as cyclic 12 (C12, n = 4) and 16 (C16, n = 4) and pregnant 12 (P12, n = 4), 16 (P16, n = 4), and 22 (P22, n = 4). Additionally, 2) ewes were grouped into four groups following treatment of PGF2α, the duration of PGF2α challenge at 1 (PG1, n = 4), 4 (PG4, n = 4), and 16 (PG16, n = 4) hours on day 12 of the cycle was compared with 0 h. Read More

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Development of a letrozole-based synchronization protocol for fixed-time artificial insemination in beef cattle.

Anim Reprod Sci 2022 May 11;240:106975. Epub 2022 Apr 11.

Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address:

Experiments were done to determine ovulation synchrony following a 4-day letrozole treatment (Exp 1), compare the efficacy of a letrozole-based protocol with other commonly used synchronization protocols for FTAI (Exp 2), and test a new intravaginal letrozole-releasing device (LRD) with and without pre-synchronization (Exp 3). In Exp 1, heifers and lactating cows at random stages of the estrous cycle were given an LRD for 4 days, PGF at LRD removal, and GnRH at 48 or 60 h after PGF, or no GnRH. In Exp 2, heifers and lactating cows were assigned to three FTAI groups: i) LRD, ii) estradiol+progesterone, iii) 5-d Co-synch+PRID. Read More

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Intrauterine infusion of blood serum of dromedary camel improves the uterine health and fertility in high producing dairy cows with subclinical endometritis.

Anim Reprod Sci 2022 May 29;240:106973. Epub 2022 Mar 29.

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address:

The blood serum of dromedary camels contains a unique type of antibodies with a high potency to neutralize toxins and to identify and inactivate some bacterial pathogens. The present study was designed to examine changes in the endometrial histology of cows with no subclinical endometritis (SE) (experiment 1) and changes in the uterine cytology and endometrial mRNA expression of COX2, IL-1β, IL-8, and iNOS following intrauterine administration of DCBS in cows with SE as compared to different common treatments (experiment 2). In addition, the effects of the intrauterine administration of DCBS were examined on the pregnancy rate in dairy cows with SE (experiment 3). Read More

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Seasonal semen quality of a local and commercial taurine cattle breeds, raised in a subtropical climate: Relationship between external morphology of the animals and climate.

Anim Reprod Sci 2022 May 4;240:106974. Epub 2022 Apr 4.

Embrapa Genetic Resources and Biotechnology, Brasília 70770-917, Brazil. Electronic address:

Livestock production is considered an important factor influencing global warming; however, climate change has also been considered one of the major factors affecting livestock production and reproduction. The present study was carried out in the Southern region of Brazil and aimed to assess the seasonal influence of climatic factors and external morphology of the animals on the semen quality. Three ejaculates were obtained from each bull (Crioulo Lageano and Angus) in summer and winter seasons. Read More

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Identification, expression and functional analysis of activin type I receptor in common Chinese Cuttlefish, Sepiella japonica.

Anim Reprod Sci 2022 May 11;240:106976. Epub 2022 Apr 11.

Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo, China. Electronic address:

Activin, a member of the transforming growth factor-β(TGF-β) superfamily, exerts its actions by binding to specific transmembrane serine/threonine kinase receptors, known as types II and I receptors. In this study, a full-length cDNA encoding for activin type Ⅰ receptor (SjActRⅠ) was cloned, characterized, and functionally studied in S.japonica. Read More

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Basic and applied features in the cryopreservation progress of bovine embryos.

Anim Reprod Sci 2022 Apr 23;239:106970. Epub 2022 Mar 23.

Center for Natural and Human Sciences, Federal University of ABC, Santo Andre, SP, Brazil; Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, SP, Brazil. Electronic address:

The expansion of the use of in vitro production techniques has revolutionized the bovine embryo market. In the last decade, we have seen the number of in vitro produced (IVP) embryos surpass the number of in vivo-derived (IVD) embryos obtained worldwide. Concomitantly, other biotechnologies were also improved, following the global trend. Read More

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Dose related inhibitor effect of enrofloxacin on in vitro feline spontaneous myometrial contractility.

Anim Reprod Sci 2022 Apr 24;239:106972. Epub 2022 Mar 24.

Depatment of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Kyrgyz-Turkish Manas University, Bishkek 720038, Kyrgyzstan.

Enrofloxacin is one of the most widely used antibacterial drugs in feline medicine. This study investigated the effects of enrofloxacin on in vitro feline spontaneous myometrial contractility at different sexual stages. Uterine samples of the 20 queen cats at different sexual periods were placed in a tissue bath, and in vitro spontaneous stretch-induced myometrial contractions were recorded for 10 min. Read More

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Effect of metritis on in-vitro uterine contractility in cows during the puerperium.

Anim Reprod Sci 2022 Apr 23;239:106971. Epub 2022 Mar 23.

Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Germany.

Aim of this study was to determine the effect of metritis on in-vitro uterine contractility. Uteri obtained from 16 euthanized Holstein-Friesian cows were divided into two groups depending on whether metritis was absent (M-, n = 6) or present (M+, n = 10). Four longitudinal and four circular myometrial strips of all uteri were incubated in an organ bath. Read More

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Evaluation of an on-farm method to assess colostrum IgG content in hyperprolific sows.

Anim Reprod Sci 2022 Apr 18;239:106958. Epub 2022 Mar 18.

Laboratory of Epidemiology, Biostatistics and Animal Health Economics, Faculty of Veterinary Medicine, School of Health Sciences, University of Thessaly, Karditsa 43100, Greece.

The colostrum quality (immunoglobulin content) is one of the most challenging aspects for hyperprolific sows in the modern pig industry. The objective of this study was to evaluate relationships among sow body condition scores, litter characteristics, and colostrum IgG content in hyperprolific sows, using a digital Brix refractometer to measure IgG. Samples (5 ml) were collected from two hundred and eighty-seven sows within the first hour postpartum. Read More

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Reproductive efficiency of treated Karakul ewes with short-term progesterone and hCG injections during the non-breeding and breeding seasons.

Anim Reprod Sci 2022 Apr 15;239:106969. Epub 2022 Mar 15.

Department of Animal Science Research, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.

The aim of this study was to evaluate reproductive efficiency of ewes following a short-term treatment with progesterone (P4) plus human chorionic gonadotropin (hCG) injections. In Exp 1, ewes (n = 65) were used during the non-breeding season and received P4, three times every other day (n = 22, 3PHCG, IM) or a single dose of P4 (n = 21, PHCG). We injected hCG in all ewes 24 h after the P4 treatment, and distilled water in control group (n = 22). Read More

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Greater numbers of antral follicles in the ovary are associated with increased concentrations of glucose in uterine luminal fluid of beef heifers.

Anim Reprod Sci 2022 Apr 15;239:106968. Epub 2022 Mar 15.

USDA, Agricultural Research Service US Meat Animal Research Center, Clay Center, NE, United States.

Increased antral follicles are associated with greater fertility and a uterine environment that is more supportive of early embryonic development in beef heifers. Glucose is a primary energy source for embryos, and glucose concentrations are elevated in uterine luminal fluid (ULF) of pregnant heifers. We hypothesized that ULF glucose concentrations and endometrial transcript abundance for glucose transporters on d16 after insemination would be greater in heifers with increased numbers of antral follicles. Read More

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Dietary carob fruit (Ceratonia siliqua L.) supplementation improves spermatogenesis, semen quality and embryonic death via antioxidant effect in aging broiler breeder roosters.

Anim Reprod Sci 2022 Apr 12;239:106967. Epub 2022 Mar 12.

Department of Animal Science, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Iran.

Rooster semen contains high content of long-chain polyunsaturated fatty acids; therefore, spermatozoa are very susceptible to lipid peroxidation. Carob fruit (CF) contains abundant amounts of flavonoid known as natural antioxidants and semen enhancers. The purpose of the study was to investigate the effect of dietary supplementation of CF on semen characters, blood parameters, immune response, testicular histology, and reproductive performance of aging broiler breeder roosters. Read More

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Advances in captive breeding and seed rearing of striped murrel Channa striata, a high value food fish of Asia.

Anim Reprod Sci 2022 Mar 25;238:106957. Epub 2022 Feb 25.

Aquaculture Production and Environment Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar 751002, India.

Striped murrel (Channa striata) is a high-value food fish in South and Southeast Asian countries with substantial medicinal properties. High growth rate, consumer preference and year-round market demand insist its candidacy in aquaculture. In addition, striped murrel is suitable for high-density intensive farming systems due to its air-breathing ability. Read More

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Success of artificial insemination based on expression of estrus and the addition of GnRH to an estradiol/progesterone-based protocol on pregnancy rates in lactating dairy cows.

Anim Reprod Sci 2022 Mar 23;238:106954. Epub 2022 Feb 23.

Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina; Instituto A.P. de Ciencias Básicas y Aplicadas, Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, Córdoba, Argentina. Electronic address:

Three experiments evaluated the effects of insemination based on estrus expression or treatment with GnRH, on the time of ovulation and pregnancy rates to AI (P/AI) in dairy cows. All cows were treated with a P4 device for 8 d, estradiol benzoate at device insertion and prostaglandin F, estradiol cypionate and eCG at device removal. In Experiment 1, P/AI was less (P < 0. Read More

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Effects of neonatal methoxychlor exposure on the ovarian transcriptome in piglets.

Anim Reprod Sci 2022 Mar 24;238:106956. Epub 2022 Feb 24.

Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address:

Methoxychlor (MXC) is a man-made organochlorine insecticide capable of disrupting endocrine functions due to its mixed steroidal properties (estrogenic, anti-estrogenic and/or anti-androgenic). Retarded follicle development was recently reported in neonatal pigs treated with MXC. The goal of the current study was to better understand the mechanism of MXC action in the ovary of newborn piglets. Read More

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Effects of maternal feed intake restriction on the blood parameters, fatty acid profile and lipogenetic genes expression of perirenal fat in offspring kids.

Anim Reprod Sci 2022 Mar 23;238:106955. Epub 2022 Feb 23.

Animal Science Department of Agriculture and Animal Husbandry College, Qinghai University, Xining, PR China. Electronic address:

Maternal nutritional restriction impacted lipogenic gene expression in adipose tissue of offspring, but the association of this programming with DNA methylation is not yet clear. Therefore, this experiment aimed to investigate the effects of maternal feed intake restriction on offsprings' blood indexes, lipid metabolism of perirenal adipose tissue (PAT) and DNA methylation of lipogenic genes. Blood and PAT were collected from the fetuses (at d 100 of gestation) and kids (at d 90 after birth). Read More

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An efficient protocol for chromosome isolation from sterlet (A. ruthenus) embryos and larvae.

Anim Reprod Sci 2022 Mar 22;238:106953. Epub 2022 Feb 22.

Department of Marine Biology and Ecology, Institute of Oceanography, University of Gdansk, Al. M. Piłsudskiego 46, 81-378 Gdynia, Poland.

In the present manuscript an efficient and feasible protocol for chromosome preparation from sterlet (A. ruthenus) embryos and larvae was developed that can be used as routine molecular diagnostic tool of the species genome manipulation procedures verification. For this end, a multi-dimensional experimental system was conceived that enabled for the establishment of chromosome preparation protocol, which characterized the mean efficiency of chromosome extraction ranged from 70% to 100% and the average number of recorded metaphases per slide ranged between 9 and 15. Read More

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What is the relevance of seminal plasma from a functional and preservation perspective?

Anim Reprod Sci 2022 Feb 17:106946. Epub 2022 Feb 17.

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil. Electronic address:

When preserving sperm in the liquid or cryopreserved state, seminal plasma (SP) components within ejaculates can alter fertilizing capacity of these gametes. Depending on the species or how semen is collected, volume and concentration of SP components varies considerably. The SP contains substances essential for maintenance of sperm viability and fertility; however, these components can be deleterious depending on quantity, or duration of time before there is removal of SP from sperm in semen processing. Read More

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February 2022

Alginate encapsulation of stallion sperm for increasing storage stability.

Anim Reprod Sci 2022 Mar 12;238:106945. Epub 2022 Feb 12.

Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany.

The aim of this study was to establish an alginate encapsulation procedure for stallion sperm, and investigate if sperm encapsulation enhances longevity during cold storage and survival after cryopreservation. First, biocompatibility of the compounds needed for encapsulation was tested and factors determining capsule structure were identified. Sperm encapsulation was realized either by depositing droplets (20 µL) of sperm solution supplemented with barium or calcium chloride (10 mM) in alginate solution (0. Read More

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Effect of hydrated C fullerene on lipid, vitamin and amino acid composition in frozen-thawed ram semen.

Anim Reprod Sci 2022 Mar 2;238:106939. Epub 2022 Feb 2.

Fırat University, Faculty of Science, Department of Chemistry, Elazığ, Turkey.

This study was conducted to investigate the effect of different doses of hydrated C fullerene (CHyFn) on freeze-thawing process-induced changes in lipid, vitamin and amino acid composition and also in motility, kinematic, sperm quality and oxidative stress parameters in ram semen. Semen was collected from seven rams twice a week for 3 weeks, so six repetitions were performed. The semen collected in each repetition was pooled. Read More

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How can mating systems inform future biobanking strategies? An illustration using two Indonesian bovids, banteng (Bos javanicus) and lowland anoa (Bubalus depressicornis).

Anim Reprod Sci 2022 Mar 9;238:106943. Epub 2022 Feb 9.

South-East Zoo Alliance for Reproduction & Conservation, 581705 White Oak Road, Yulee, FL 32097, USA.

Storing cryopreserved spermatozoa in a genome resource bank safeguards against the loss of heterozygosity in endangered species and provides opportunities to reincorporate genes into populations through the application of assisted reproductive technologies. The aim of this study was to demonstrate the effect of breeding strategy on ejaculate characteristics to illustrate how this information may be used to select appropriate methods for the storage and use of cryopreserved sperm. In the present study, ejaculates from a polygynous bovid, banteng (Bos javanicus), were characterized (motility 72. Read More

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Artificial reproduction of Caspian roach, Rutilus caspicus following stimulating ovulation with Ovaprim, Ovopel, and their combinations under controlled conditions.

Anim Reprod Sci 2022 Mar 21;238:106932. Epub 2022 Jan 21.

Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.

The aim of this study was to evaluate the efficiency of different hormonal agents and their combinations for spawning induction of females Caspian roach, Rutilus caspicus. Five groups of fish were injected intraperitoneally as follows: two injections of Ovaprim (T1), two injections of Ovopel (T2), a priming dose of Ovopel with a resolving dose of Ovaprim (T3), a priming dose of Ovaprim with a resolving dose of Ovopel (T4), and two injections of sterile 0.9% NaCl solution as a control group. Read More

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Identification of vaginal Leptospira in cervical-vaginal mucus of slaughtered pigs in the amazon region.

Anim Reprod Sci 2022 Mar 19;238:106930. Epub 2022 Jan 19.

Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Prof. Hernani Mello St., 101, São Domingos, 24210-130 Niterói, Rio de Janeiro, Brazil.

Swine genital leptospirosis is an infectious disease that leads to economic losses due to abortions, stillbirths, and reproductive failures. Considering the scarcity of studies regarding this condition, the objective of the present study was to identify and analyse leptospires infecting the reproductive tract of female pigs slaughtered in the Amazon region. Cervical-vaginal mucus (CVM) from 150 non-pregnant females were collected and submitted to molecular analysis. Read More

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Exposure to non-esterified fatty acids in vitro results in changes in the ovarian and follicular environment in cattle.

Anim Reprod Sci 2022 Mar 4;238:106937. Epub 2022 Feb 4.

Department of Animal Science, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA. Electronic address:

Negative energy balance (NEB) in the postpartum period of dairy cows is associated with reduced fertility to insemination later in lactation. We hypothesized that elevated non-esterified fatty acids (NEFA) levels that occur during NEB result in accumulation of fatty acids within the ovarian tissue and preantral follicles, causing changes in ovarian gene expression that would indicate a response to injury. We performed ovarian cortex culture and oocyte maturation in medium containing a combination of palmitic, oleic and stearic acid (NEFA). Read More

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Past, present and future of ICSI in livestock species.

Anim Reprod Sci 2022 Jan 19:106925. Epub 2022 Jan 19.

Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Buenos Aires, Laboratorio Biotecnología Animal (LabBA), Av. San Martin 4453, Ciudad Autónoma de, Buenos Aires 1417, Argentina; CONICET-Universidad de Buenos Aires, Instituto de Investigaciones en Producción Animal (INPA), Buenos Aires, Argentina. Electronic address:

During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine technique for clinical applications in humans. The widespread use among domestic species, however, has been limited to horses. In horses, ICSI is used to reproduce elite individuals and, as well as in humans, to mitigate or even circumvent reproductive barriers. Read More

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January 2022