4,373 results match your criteria Animal Reproduction Science[Journal]


On-farm lambing outcomes after transfer of vitrified and slow frozen embryos.

Anim Reprod Sci 2020 May 18;216:106467. Epub 2020 Apr 18.

Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Thailand. Electronic address:

The aim of the present study was to determine the most suitable embryonic stage and embryo freezing technique for commercial implementation of frozen embryo trading by small-scale sheep producers. There was a 2 × 2 factorial design utilized for conducting the study consisting of two embryo stages (2-8 cells or morula/blastocyst) and two cryopreservation protocols (vitrification or slow-freezing). For the in vivo produced embryos, there were treatments of crossbred donor ewes to induce superovulation. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106467DOI Listing

Can accelerometer ear tags identify behavioural changes in sheep associated with parturition?

Anim Reprod Sci 2020 May 4;216:106345. Epub 2020 Apr 4.

Institute for Future Farming Systems, CQ University, Rockhampton QLD 4701 Australia.

On-animal sensor systems provide an opportunity to monitor ewes during parturition, potentially reducing ewe and lamb mortality risk. This study investigated the capacity of machine learning (ML) behaviour classification to monitor changes in sheep behaviour around the time of lambing using ear-borne accelerometers. Accelerometers were attached to 27 ewes grazing a 4. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106345DOI Listing

Fetal programming in dairy cows: Effect of heat stress on progeny fertility and associations with the hypothalamic-pituitary-adrenal axis functions.

Anim Reprod Sci 2020 May 4;216:106348. Epub 2020 Apr 4.

Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL)/Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina. Electronic address:

Ambient temperatures that result in body temperatures beyond those of the thermo-neutral zone for dairy cattle can lead to reduced reproductive efficiencies that have negative effects on economic and productive efficiencies of dairy farms. In addition, in pregnant cows, ambient temperature-induced heat stress leads to modifications in the epigenome of the developing embryo, which, in turn, could lead to phenotypic variations in the sexually mature animal and its offspring. In the mammalian response to stress, adrenocorticotropic hormone stimulates the synthesis and secretion of glucocorticoids, which may have detrimental effects on the hypothalamic-pituitary-gonadal axis and the female estrous cycle. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106348DOI Listing

Vincristine and ivermectin combination chemotherapy in dogs with natural transmissible venereal tumor of different cyto-morphological patterns: A prospective outcome evaluation.

Anim Reprod Sci 2020 May 6;216:106358. Epub 2020 Apr 6.

Teaching Hospital of Veterinary Medicine, Federal University of Bahia (UFBA), Salvador, Bahia, 40170-110, Brazil; Department of Veterinary Anatomy, Pathology and Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, Bahia, 40170-110, Brazil. Electronic address:

Vincristine is the first-line drug for the chemotherapy of canine transmissible venereal tumor (CTVT). Drug resistance is related to tumor cyto-morphological patterns of CTVT. There are anti-cancer properties of ivermectin, thus, a combination of ivermectin and vincristine could be an effective chemo-therapeutic treatment regimen for CTVT. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106358DOI Listing

17β-estradiol regulates prostaglandin E and F synthesis and function in endometrial explants of cattle.

Anim Reprod Sci 2020 May 18;216:106466. Epub 2020 Apr 18.

Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China. Electronic address:

Prostaglandins (PG) have primary functions in the reproductive tract, however, the mechanism of regulation of PG secretion in the endometrium is unclear. Estrogen as a predominant regulator of uterine functions during the mammalian estrous cycle and effects of estrogen on synthesis of PG and function in uterine tissues of cattle are not fully understood. In this study, there was evaluation of the concentration- and time-effects of 17β-estradiol on PG synthesis in endometrial explants of cattle, focusing on the secretion of prostaglandin E (PGE) and prostaglandin F (PGF) as well as relative abundance of mRNA transcript and protein for both the enzymes responsible for PGE and PGF synthesis, including prostaglandin-endoperoxide synthase 1 and 2 (PTGS1, PTGS2), PGE synthase (PGES), PGF synthase (PGFS), and carbonyl reductase (CBR1), and the receptors responsible for downstream PGE (PTGER2, PTGER4) and PGF (PTGFR) signaling. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106466DOI Listing

Effect of relaxin on semen quality variables of cryopreserved stallion semen.

Anim Reprod Sci 2020 May 2;216:106351. Epub 2020 Apr 2.

Department of Veterinary Science, University of Torino, Grugliasco, Italy.

The aim of the study was to ascertain effects of different concentrations of relaxin added to extender medium during the pre-freezing incubation periods on quality variables of stallion frozen-thawed spermatozoa. Semen samples collected from three stallions were filtered, diluted with skim milk, and centrifuged at 600g for 10 min. Sperm pellets were suspended in BotuCrio freezing medium to a final concentration of 50 × 10 sperm/mL. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106351DOI Listing

Growth of the conceptus from days 33 to 45 of pregnancy is similar for heifers and lactating cows and not associated with circulating glucose, insulin, IGF1 or progesterone concentrations.

Anim Reprod Sci 2020 May 18;216:106463. Epub 2020 Apr 18.

Division of Animal Sciences, University of Missouri, 160 Animal Science Research Center, 920 East Campus Drive, Columbia, MO 65211, United States. Electronic address:

Less than optimal circulating progesterone as well as glucose, insulin, and IGF1 concentrations in lactating cows may contribute to embryonic loss if placental development is dependent on concentrations of these hormones. There was comparison of fetal growth in lactating dairy cows with a contemporary group of nulliparous heifers (negative control for lactation). There was also assessment of the association between conceptus development and circulating hormone and metabolite concentrations and the response to an intravenous glucose administration. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106463DOI Listing

Treatment of cows that fail to respond to pre-synchronization treatments with a CIDR-Ovsynch regimen improves the overall pregnancy percentage after a double Ovsynch treatment regimen.

Anim Reprod Sci 2020 May 6;216:106356. Epub 2020 Apr 6.

Department of Surgery, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Al Sharquiya Governorate, Egypt.

In this study, there was evaluation of pregnancy per AI (P/AI) as a result of the first postpartum AI following four ovulation-synchronization treatments. Treatment regimens were Ovsynch-56 (OVS, n = 875; GnRH1-7d-PGF-56h-GnRH2-16h-FTAI), CIDR-OVS (n = 1001; OVS plus CIDR inserts between GnRH1 and PGF), Double-Ovsynch (DOVS, n = 663; imposing Pre-OVS followed by breeding-OVS 7 days later), and Modified-DOVS (M-DOVS, n = 1397; imposing Pre-OVS followed either by breeding-OVS or CIDR-OVS based upon the ovarian structure at GnRH1). Cows with a corpus luteum were assigned to a breeding-OVS treatment regimen and those that did not have a corpus luteum were assigned to the CIDR-OVS treatment regimen. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106356DOI Listing

Genetic disorders of sex development in cats: An update.

Anim Reprod Sci 2020 May 5;216:106353. Epub 2020 Apr 5.

Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland. Electronic address:

Disorders of sex development (DSD) are rarely reported in cats, but this does not mean these occurrences are an insignificant reproductive and health problem in this species. The DSD condition affects reproduction and can be associated with an increased risk of gonadal tumorigenesis. In this review, an overview of findings since 2012 are presented that focus on cytogenetic and molecular genetic studies of cats with abnormal external genitalia. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106353DOI Listing

Effect of oral urea supplementation on the endometrial transcriptome of mares.

Anim Reprod Sci 2020 May 18;216:106464. Epub 2020 Apr 18.

Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, 40546, USA. Electronic address:

An intravenous large dose of protein led to an increased blood urea nitrogen (BUN), resulting in a lesser uterine pH and altered uterine gene expression in mares. The objective of the present study was to evaluate effects of a more physiological methodology to increase BUN on the endometrium of mares. Mares were fed hay and a treatment or control diet (n = 11 mares/treatment) in a crossover design starting at time of ovulation detection (D0) and continuing until D7. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106464DOI Listing

Reproductive performance in three Slovenian sheep breeds with different alleles for the MTNR1A gene.

Anim Reprod Sci 2020 May 4;216:106352. Epub 2020 Apr 4.

Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. Electronic address:

The aim of the research was to describe the polymorphisms of the MTNR1A gene in three Slovenian sheep breeds, and determine whether these polymorphisms affect reproductive performance. A total of 100 Bovška, 110 Istrian Pramenka, and 108 Jezersko-Solčavska ewes, were used. The rams were placed with the ewes on 10 October (Bovška), and 20 September (Istrian Pramenka). Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106352DOI Listing

Long-term storage of boar seminal doses contaminated with Proteus vulgaris: A dose-dependent effect on sperm motility and sperm-bacteria interaction.

Anim Reprod Sci 2020 May 9;216:106349. Epub 2020 Apr 9.

Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, C/Maria Aurèlia Campany, 69, Campus de Montilivi, 17003, Girona, Spain. Electronic address:

This study evaluated how Proteus vulgaris affects sperm quality and sperm-bacteria interaction in stored semen samples. A strain of P. vulgaris resistant to streptomycin, penicillin, lincomycin and spectinomycin was added to boar semen in doses of 10, 10, 10, 10 and 10 CFU/mL. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106349DOI Listing

Physiologic responses to feeding rumen-protected glucose to lactating dairy cows.

Anim Reprod Sci 2020 May 4;216:106346. Epub 2020 Apr 4.

Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506-0201, United States. Electronic address:

It was hypothesized that rumen-protected glucose (RPG) in diets of dairy cows increases concentrations of insulin resulting in greater blood progesterone concentrations because elevated insulin decreases activity of liver enzymes inactivating steroid hormones. Timing of ovulation was synchronized among 64 postpartum Holstein cows using GnRH and PGF (Day 0 = ovulation). Cows were milked thrice daily and assigned randomly a basal diet supplemented with 0, 1, 2, or 4 kg of an RPG product in place of corn grain, top-dressed in the diet beginning on Day -3. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106346DOI Listing

Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa.

Anim Reprod Sci 2020 May 2;216:106344. Epub 2020 Apr 2.

Nucleus of Research in Food Production, Faculty of Natural Resources, Catholic University of Temuco, Temuco, Chile. Electronic address:

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (ΔΨMit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into three groups: Control (C), T1 (45/90 % Percoll®) and T2 (45/60 % Percoll®). Sperm motility was evaluated using CASA (Computer-Assisted Sperm Analysis), ΔΨMit using flow cytometry, and fertility evaluating whether cleavage of fertilised eggs had occurred after 16 h of incubation at 10 °C. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106344DOI Listing

Association of fertility with group mating behavior in ewes.

Anim Reprod Sci 2020 May 1;216:106359. Epub 2020 Apr 1.

Reproduction, Animal Science, Invermay Agricultural Centre, AgResearch Ltd, Mosgiel, 9092, New Zealand.

It was hypothesized that values for fertility variables would vary with number of rams ewes mated with in a group mating management system. In Experiment 1, for adult ewes (n = 872) joined with rams for 35-38 days, 19.0 ± 2. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106359DOI Listing

Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep.

Anim Reprod Sci 2020 May 2;216:106347. Epub 2020 Apr 2.

Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey. Electronic address:

The aim of this study was to investigate relative abundance and localization of ISG15 mRNA transcript in intra-uterine (trophoblast, endometrium) and extra-uterine (hypothalamus, anterior pituitary, corpus luteum) tissues before and during the period of conceptus implantation. Multiparous ewes (n = 16) were randomly allotted into four groups: pregnant or estrous cyclic on days of 12 and 16 (n = 4 per group) following estrus. Relative abundances of ISG15 mRNA transcript were determined in the endometrium, corpus luteum, hypothalamus, and anterior-pituitary using real time quantitative PCR. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106347DOI Listing

Persian onager (Equus hemionus onager) endometrial explant cryopreservation and in vitro culture.

Anim Reprod Sci 2020 Jun 25;217:106459. Epub 2020 Apr 25.

Smithsonian Conservation Biology Institute, Center for Species Survival, 1500 Remount Road, Front Royal, VA 22630, USA. Electronic address:

Assisted reproduction of endangered equids, such as Persian onagers (Equus hemionus onager), is vital for species conservation. Little is known about Persian onager reproductive functions, including functions of the uterine endometrium. Recently, successful cryopreservation of the domestic mare endometrium was reported, but there is no information on cryo-sensitivity or in vitro culture of endometrial tissues of any non-domestic equid. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106459DOI Listing

GnRHa treatments of Atlantic Salmon broodstock suppresses effects of endocrine disruptors, benefitting offspring quality.

Anim Reprod Sci 2020 Jun 21;217:106470. Epub 2020 Apr 21.

Facultad de Ingeniería y Ciencias, Departamento de Ingeniería Química, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.

The use of synthetic hormones to regulate sexual maturity in captive fish is a common practice. With aquaculture practices, fish production is desired throughout the year, necessitating the maintenance of quality standards, mainly regarding the characteristics of the fish produced. Embryonic development may be affected by toxins in the environment and by a variety of pathologies. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106470DOI Listing

Repeated trans-cervical embryo recoveries in Santa inês ewes subjected to short- or long-term superovulatory treatment regimens.

Anim Reprod Sci 2020 Jun 20;217:106469. Epub 2020 Apr 20.

Embrapa Goats and Sheep Research Center, Estrada Sobral/Groaíras, Km 04, CP 145, CEP 62010-970, Sobral, CE, Brazil.

Outcomes of short- (6.5 days) and long-term (14.5 days) estrous synchronization for 6. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106469DOI Listing

Reduction of spermatophore melanization in Litopenaeus vannamei shrimp fed Ulva clathrata during a commercial hatchery production.

Anim Reprod Sci 2020 Jun 20;217:106468. Epub 2020 Apr 20.

Programa de Acuicultura, Centro de Investigaciones Biológicas del Noroeste, La Paz, Baja California Sur, 23000, Mexico. Electronic address:

Male broodstock (Litopenaeus vannamei, 36 ± 7 g, n = 600) reproductive performance, spermatophores and reproductive tract melanization, prostaglandin concentrations and biochemical composition were evaluated after including 3.8% Ulva clathrata meal in the diet (dry base) of a commercial hatchery during 45 days. Males fed Ulva had less melanized spermatophores (120 compared with 233, P < 0. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106468DOI Listing

Soy lecithin as a potential alternative to powdered egg yolk for buck sperm cryopreservation does not protect them from mitochondrial damage.

Anim Reprod Sci 2020 Jun 23;217:106473. Epub 2020 Apr 23.

Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Spain. Electronic address:

The aim of this study was to address whether soy lecithin (SL) was an effective non-penetrating cryoprotectant for buck sperm cryopreservation in the presence of seminal plasma. There was also an attempt to determine the optimal concentration of BHT as an antioxidant in powdered egg yolk (PEY) or in SL based media. Two ejaculates were collected from six bucks and mixed ejaculates were aliquoted into washed, using centrifugation procedures, and unwashed samples. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106473DOI Listing

Effect of abortion on milk production, health, and reproductive performance of Holstein dairy cattle.

Anim Reprod Sci 2020 Jun 27;217:106458. Epub 2020 Apr 27.

AbacusBio Ltd., Ground Floor, Public Trust Building, 442 Moray Place, P.O. Box 5585, Dunedin, New Zealand.

The objective of this study was to identify the effect of abortion on performance of Iranian Holstein cows that calved between 2005-2014. Abortion records were categorized in two groups: 1) new abortion (NLA), and 2) rebreeding abortion (RA). Production data (305-d milk, fat, and protein yields) were analyzed using PROC MIXED, number of inseminations per lactation period (INS), postpartum health disorders (PPHD), and culling records using PROC GLIMMIX, and calving to conception records using PROC PHREG of SAS software (SAS Institute, 2013). Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106458DOI Listing

Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cows.

Anim Reprod Sci 2020 Jun 28;217:106455. Epub 2020 Apr 28.

Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan. Electronic address:

Changes in the C-reactive protein (CRP) concentration, a non-specific diagnostic biomarker, in uterine lavage fluid and associations with cytological findings were examined following parturition in dairy cows. In postpartum Holstein dairy cows (n = 8), uterine lavage was performed at 3 and 6 weeks postpartum, and polymorphonuclear leukocyte (PMN) ratios to total cells (PMN:ALL) and to lymphocytes (PMN:LYM) and CRP concentrations were determined. Blood samples were collected to monitor the metabolic variables, and plasma CRP concentrations were quantified using samples collected at the same time as the uterine lavage occurred. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106455DOI Listing

Effects of vulvar width and antral follicle count on oocyte quality, in vitro embryo production and pregnancy rate in Bos taurus taurus and Bos taurus indicus cows.

Anim Reprod Sci 2020 Jun 27;217:106357. Epub 2020 Apr 27.

Animal Science Department, Federal University of Lavras, Lavras, MG, Brazil. Electronic address:

It was hypothesized that Brahman cows have greater in vitro embryo production than Simmental cows, and that regardless of breed, antral follicle count (AFC) and vulvar width (VW) are positively associated with oocyte quality, embryo number and pregnancy rate. Brahman (Bos taurus indicus, n = 184) and Simmental (Bos taurus taurus, n = 99) cows were classified as having a large AFC (Brahman: ≥50, Simmental: ≥25); intermediate AFC (Brahman: >30 and ≤49; Simmental: >16 and <24) and small AFC (Brahman: <30, Simmental: ≤15). The VW was considered to be large (Brahman: >123 mm, Simmental: >80 mm); intermediate (Brahman: >102 mm and ≤123 mm; Simmental: >65 mm and ≤80 mm) and small (Brahman: ≤102 mm and Simmental: ≤65 mm). Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106357DOI Listing

Functions and effects of reactive oxygen species in male fertility.

Anim Reprod Sci 2020 Apr 26:106456. Epub 2020 Apr 26.

Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia.

Reactive oxygen species (ROS) are implicated in all aspects of cellular functions. While the importance of ROS as signalling molecules is well described, ROS are also associated with stress pathologies. Within the reproduction field, there are associations with reduced fertility as a result of lipid peroxidation, protein dysfunction, premature cell death and DNA damage which readily occur in spermatozoa. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106456DOI Listing

Letter from the Guest Editors.

Anim Reprod Sci 2020 Apr 10:106462. Epub 2020 Apr 10.

Department of Production Animal Health, University of Calgary, Faculty of Veterinary Medicine, Calgary, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.anireprosci.2020.106462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194949PMC

Computer Aided Sperm Analysis (CASA) in domestic animals: Current status, three D tracking and flagellar analysis.

Anim Reprod Sci 2020 Apr 4:106350. Epub 2020 Apr 4.

Department of Medical Bioscience, Comparative Spermatology Laboratory, University of the Western Cape, Robert Sobukwe Rd., P/B X17, Bellville, 7535, South Africa. Electronic address:

Computer Aided Sperm Analysis is currently well established in domestic animals. Apart from sperm concentration and sperm motility assessment (percentage groupings, kinematics groupings) sperm morphology, sperm viability, sperm fragmentation and the acrosome reaction are automated as part of modern CASA systems. This review cum new original research paper focuses on providing baseline data on sperm concentration and motility in common domestic species of animals of proven fertility including bull, boar, horse, ram, goat, dog, donkey, chicken. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106350DOI Listing

Metabolomes of sperm and seminal plasma associated with bull fertility.

Anim Reprod Sci 2020 Apr 1:106355. Epub 2020 Apr 1.

Department of Reproduction and Artificial Insemination, Selcuk University, Turkey.

Bull fertility is crucial for efficient, profitable, and sustainable agriculture of cattle. Despite the fact that the uses of sperm from low fertility bulls cause millions of dollars economic losses, conventional methods available to accurately predict bull fertility are still of limited use. Although breeding bulls produce billions of sperm mostly with normal motility and morphology, some bulls are afflicted with poor fertility due to molecular or cellular defects in the sperm. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106355DOI Listing

Effects of abundances of OCT-4 mRNA transcript on goat pre-implantation embryonic development.

Anim Reprod Sci 2020 Apr 1;215:106286. Epub 2020 Feb 1.

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address:

Unlike in mice, the function of pluripotent markers in early embryonic development of domestic animals remains to be elucidated and this may account for the failure to establish embryonic stem cell lines for these species. To study the functions of the OCT-4 protein which has important actions in maintenance of pluripotent and self-renewal processes during early embryonic development, there was induced reduction in relative abundance of OCT-4 mRNA transcript during goat early embryonic development by using RNA interference techniques. The injection of OCT-4 siRNA into goat IVF presumptive zygotes resulted in a decrease in the relative abundance of OCT-4 mRNA transcript; however, there was development of these embryos to the blastocyst stage at the same rate as there was in the control group. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106286DOI Listing

Molecular characteristics and abundance of insulin-like androgenic gland hormone and effects of RNA interference in Eriocheir sinensis.

Anim Reprod Sci 2020 Apr 25;215:106332. Epub 2020 Feb 25.

Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang, 262700, China.

Insulin-like androgenic gland hormone (IAG) has an important function in sexual differentiation and somatic growth in crustaceans. In this study, there was cloning of the full-length sequence of IAG from Eriocheir sinensis (Es-IAG). The full-length Es-IAG gene was 1392 base pairs long and encoded a protein of 151 amino acid residues. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106332DOI Listing

Estradiol-17β regulates proliferation and apoptosis of sheep endometrial epithelial cells by regulating the relative abundance of YAP1.

Anim Reprod Sci 2020 Apr 21;215:106328. Epub 2020 Feb 21.

Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China; Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Yes-associated protein 1 (YAP1) transcription regulator of the Hippo protein kinase pathway, serves as a key regulator of tissue growth and organ size by regulating cell proliferation and apoptosis. Effects of YAP1 on proliferation and apoptosis of sheep endometrial epithelial cells (EEC) as a result of estradiol-17β (E) treatment, however, remain unclear. In the present study, the abundance of YAP1 protein in the uterine horn was greater than that in the uterine body or cervix. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106328DOI Listing

Optimization of sterlet (Acipenser ruthenus) egg incubation.

Anim Reprod Sci 2020 Apr 26;215:106334. Epub 2020 Feb 26.

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic. Electronic address:

Sterlet Acipenser ruthenus was used to assess egg and embryo development when incubated at 17 °C in Petri dishes placed in a hatchery tank (300 L recirculating dechlorinated water) with incubation occurring in a static tabletop system in an air-conditioned laboratory, or in a 700 L Q-cell incubator. Eggs in each dish were placed in a plastic box with 300 mL dechlorinated water. Separated eggs from three individual females were fertilized using pooled sperm from four males with there being four replicates. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106334DOI Listing

Effects of intra-uterine infusion of proteolytic enzymes on selected cytokine concentrations, uterine inflammation, and fertility in postpartum water buffalo cows with subclinical endometritis.

Anim Reprod Sci 2020 Apr 27;215:106335. Epub 2020 Feb 27.

Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

The study was conducted to evaluate effects of intrauterine administration of proteolytic enzymes on endometrial inflammation and reproductive performance in postpartum water buffalo cows with subclinical endometritis (SCE). Cows (n = 38) with SCE (≥ 18 % PMN i.e. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106335DOI Listing
April 2020
1.511 Impact Factor

Cortisol inhibits the Escherichia coli-induced endometrial inflammatory response through NF-κB and MAPK pathways in postpartum goats.

Anim Reprod Sci 2020 Apr 25;215:106333. Epub 2020 Feb 25.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Rd., Yangzhou, Jiangsu, 225009, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, 12 East Wenhui Rd., Jiangsu, 225009, China. Electronic address:

Glucocorticoids have been widely used as anti-inflammatory therapies. The mechanisms of cortisol action in goat does with endometritis, however, have not been reported. The aim of this study was to investigate the mechanism of cortisol in modulation of effects of E. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106333DOI Listing
April 2020
1.511 Impact Factor

Heterotopic ovarian allotransplantation in goats: Preantral follicle viability and tissue remodeling.

Anim Reprod Sci 2020 Apr 5;215:106310. Epub 2020 Feb 5.

Laboratory of Diagnostic Imaging Applied to Animal Reproduction, State University of Ceará, Fortaleza, CE, Brazil. Electronic address:

An appropriate implantation site favors angiogenesis and avoids ovarian tissue damage after tissue grafting. The objective of this study was to evaluate the effects of intramuscular (IM) and subcutaneous (SC) sites for ovarian grafts in goats by evaluating follicular morphology and activation, preantral follicle and stromal cell densities, tissue DNA fragmentation, collagen types I and III depositions, and graft revascularizations. Ovarian cortical tissue was transplanted in IM or SC sites and recovered 7 or 15 days post-transplantation. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106310DOI Listing

Motile sperm subpopulations in bull semen using different clustering approaches - Associations with flow cytometric sperm characteristics and fertility.

Anim Reprod Sci 2020 Apr 21;215:106329. Epub 2020 Feb 21.

Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland.

There are sperm subpopulations (SPs) with different kinematic characteristics in various species, however, biological relevance of these SPs is still uncertain. The objective of the present study was to investigate associations of motile sperm SPs with sperm characteristics determined by evaluations with flow cytometry and assessment of bull fertility, using multiple approaches for sperm clustering. Semen from 24 bulls was evaluated concomitantly using computer-assisted sperm analysis (CASA) and flow cytometry before freezing and after thawing. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106329DOI Listing

Effects of selenium on the proliferation and apoptosis of sheep spermatogonial stem cells in vitro.

Anim Reprod Sci 2020 Apr 22;215:106330. Epub 2020 Feb 22.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, PR China; Laboratory of Animal Reproductive Biotechnology, Shanxi Agricultural University, Taigu, 030801, PR China. Electronic address:

The objective of this study was to investigate effects of selenium (Se) on proliferation and apoptosis of sheep spermatogonial stem cells (SSC) in vitro. The SSC were assigned to five treatment groups (0, 2.0, 4. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106330DOI Listing

Effect of supplementation with organic and inorganic minerals on the performance, egg and sperm quality and, hatching characteristics of laying breeder hens.

Anim Reprod Sci 2020 Apr 4;215:106309. Epub 2020 Feb 4.

Poultry Science Laboratory, Department of Animal Science, Federal University of Santa Maria, Avenida Roraima Nº 1000, ZIP Code: 97105-900, Santa Maria, RS, Brazil.

This experiment was conducted to evaluate the effects of dietary supplementation with organic micro-minerals on egg production, egg quality, sperm quality and hatching of eggs of laying breeder hens. A total of 144 White Plymouth Rock hens and 36 Red Rhode Island roosters were used. For each treatment, birds were assigned to eight replicates with six hens and 12 replicates with one rooster. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106309DOI Listing

Diagnostic evaluation of subclinical endometritis in dromedary camels.

Anim Reprod Sci 2020 Apr 28;215:106327. Epub 2020 Feb 28.

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Egypt; Department of Public Health, Microbiology, College of Public Health, Qassim University, Qassim, Saudi Arabia.

The objective of the present study was to evaluate the efficacy of various diagnostic methods and to estimate the prevalence of bacterial pathogens associated with subclinical endometritis (SCE) in dromedary camels. During two consecutive breeding seasons, a total of 2122 infertile female dromedaries were assigned to this study and suspected cases of SCE were identified using the established criteria which included failure to conceive after three or more consecutive matings with a fertile male, a clinically healthy genital system, no observable vaginal discharge, and normal sexual behavior. Manual vaginal examination, Metricheck, bacteriological examination using endometrial swabbing, and hemogram assessments were conducted and there were comparisons of results to when there was cytological examination using the Cytobrush technique as the gold standard. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106327DOI Listing
April 2020
1.511 Impact Factor

In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen.

Anim Reprod Sci 2020 Apr 27;215:106315. Epub 2020 Feb 27.

Department of Animal Medicine and Surgery, Autonomous University of Barcelona, E-08193, Cerdanyola Del Vallès, Spain. Electronic address:

The study was conducted to compare the effect of four commercially available extenders (Triladyl®- egg yolk-based; Andromed® and Bioxcell®-plant based and Optixcell®-liposome-based) on post-thaw sperm quality and functionality variables evaluated using computer-assisted sperm analysis and flow cytometry. A total of 30 ejaculates from five bulls were analysed. With use of Triladyl®, sperm had a greater post-thaw total motility than with use of Bioxell® and Optixcell® but there was no difference as compared with use of Andromed® with the greatest (P < 0. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106315DOI Listing
April 2020
1.511 Impact Factor

Can maintenance intake reduce production costs without compromising the reproductive performance in bucks?

Anim Reprod Sci 2020 Apr 22;215:106331. Epub 2020 Feb 22.

Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brazil.

Excessive intake of nutrients can result in decreases in the reproductive efficiency of goat bucks and increase production costs in goat e nterprises. In this study, the aim was to compare the reproductive features of young bucks fed diets for maintenance of growth or were fed ad libitum. Ten bucks were used (n = 5/group), with a completely randomized design. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106331DOI Listing

Environmental epigenetics and epigenetic inheritance in domestic farm animals.

Anim Reprod Sci 2020 Feb 18:106316. Epub 2020 Feb 18.

Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, USA. Electronic address:

Epigenetics refers to molecular factors and processes around DNA that can affect genome activity and gene expression independent of DNA sequence. Epigenetic mechanisms drive developmental processes and have also been shown to be tied to disease development. Many epigenetic studies have been done using plants, rodent, and human models, but fewer have focused on domestic livestock species. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106316DOI Listing
February 2020

Adverse effects of in vitro manipulation of spermatozoa.

Anim Reprod Sci 2020 Feb 14:106314. Epub 2020 Feb 14.

Departments of Experimental and Clinical Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy. Electronic address:

Development of in vitro reproduction techniques has not only offered some infertile couples the possibility to have a child, it also revolutionized animal reproduction. Although in vitro reproduction techniques for humans or domestic and non-domestic animals have been designed to mimic in vivo conditions, modifications due to environmental effects or in vitro manipulation of gametes and embryos are unavoidable. For male gametes, in vitro manipulations include techniques to select spermatozoa, cryopreservation and other incubation procedures, during which spermatozoa may be exposed to oxidative stress and other insults that may damage their functions and DNA. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106314DOI Listing
February 2020

Effect of FSH treatment on cumulus oocyte complex recovery by ovum pick up and in vitro embryo production in beef donor cows.

Anim Reprod Sci 2020 Mar 8;214:106274. Epub 2020 Jan 8.

Instituto de Reproducción Animal Córdoba (IRAC), General Paz, PC.5145, Córdoba, Argentina; Instituto de Ciencias Básicas, Carrera de Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, PC.5963, Córdoba, Argentina. Electronic address:

This study was conducted to evaluate oocyte recovery and in-vitro blastocyst production of donor cows superstimulated for ovarian follicular development with FSH administered as twice-daily injections in saline or a single injection diluted in 0.5 % hyaluronan before oocyte aspiration. In Experiment 1, cows were treated with 160 mg of Folltropin-Vdiluted in saline, administered in four twice-daily i. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106274DOI Listing

Rearing and maturation of Lophiosilurus alexandri (Steindachner, 1876) in controlled conditions: First reports and future perspective.

Anim Reprod Sci 2020 Mar 6;214:106311. Epub 2020 Feb 6.

Universidade Federal de Minas Gerais, Escola de veterinária, Departamento de Zootecnia, Laboratório de Aquacultura, Avenida Antônio Carlos, 6627, CEP 30161-970, Brazil. Electronic address:

Lophiosilurus alexandri is a freshwater carnivorous fish for which there is fishing pressure in its natural environment, yet the species has potential for captive rearing. Information on growth and sexual development for the species is scarce, and age and size at first maturation have yet to be determined. A total of 400 fish, therefore, were reared from hatching to 968 days after hatching (DAH). Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106311DOI Listing

Effects of Theileria orientalis Ikeda type infection on libido and semen quality of bulls.

Anim Reprod Sci 2020 Mar 6;214:106312. Epub 2020 Feb 6.

School of Veterinary Science, Massey University, Palmerston North, New Zealand. Electronic address:

There is an epidemic in New Zealand of infectious bovine anaemia associated with Theileria orientalis Ikeda type, an obligate intracellular protozoan parasite. To establish whether T. orientalis Ikeda type infection adversely affects fertility of bulls used for natural mating, a randomised controlled experimental study was conducted. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106312DOI Listing

Effects of estrous expression and intensity of behavioral estrous symptoms on variables associated with fertility in beef cows treated for fixed-time artificial insemination.

Anim Reprod Sci 2020 Mar 4;214:106308. Epub 2020 Feb 4.

Department of Animal Science, Texas A&M University, College Station, TX, 77843-2471, United States.

This experiment was conducted to evaluate effects of estrous expression and intensity of behavioral estrous expression, assessed by evaluating physical activity, on variables associated with fertility in beef cows. The following treatment regimen was imposed on lactating, multiparous cows (n = 273): 100-μg injection of GnRH and an intravaginal progesterone (P4) releasing device (CIDR) administered on d -10, 25-mg injection of prostaglandin F at CIDR removal on d -3, and another GnRH injection + fixed-time artificial insemination (TAI) 60-66 h following CIDR removal (d 0). Cows were fitted with a pedometer on d -10, and an estrous detection patch on the tail-head on d -3. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106308DOI Listing

Characteristics of seasonal spermatogenesis in the soft-shelled turtle.

Anim Reprod Sci 2020 Mar 31;214:106307. Epub 2020 Jan 31.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China. Electronic address:

Spermatogenesis in reptiles is a seasonally dependent physiological process that is not temporally associated with male mating behavior. Characteristics of seasonal spermatogenesis in reptiles, however, remain largely unknown. In this review, there is a coverage of the characteristics of soft-shelled turtle, Pelodiscus sinensis, during seasonal spermatogenesis that provides insights into spermatogenesis of testudines. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106307DOI Listing
March 2020
1.511 Impact Factor

Decreased abundance of GDNF mRNA transcript in the immature Sertoli cells of cattle in response to protein kinase inhibitor staurosporine.

Anim Reprod Sci 2020 Mar 28;214:106303. Epub 2020 Jan 28.

College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Sertoli cells (SC) have important functions in spermatogenesis by regulating development of spermatogenic cells. Glial cell line-derived neurotrophic factor (GDNF) are produced by SC. Although the effects of GDNF on spermatogenesis have been well studied, the understanding of how GDNF is synthesized is still limited, especially in food animal producing species. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106303DOI Listing
March 2020
1.511 Impact Factor