58 results match your criteria Animal Reproduction[Journal]


Inflammation: friend or foe of bovine reproduction?

Anim Reprod 2019 Oct 24;16(3):539-547. Epub 2019 Oct 24.

Université de Tours, UMR85 Physiologie de la Reproduction et des Comportements, Centre INRA Val-de-Loire, Nouzilly, France.

Inflammation is not only the first line of defense of the organism but is also required in many reproductive processes such as ovulation, corpus luteum development, luteolysis, uterine clearance after insemination and post partum. Nevertheless, if excessive or persistent, inflammation can switch from a positive mechanism to a deleterious process, impairing oocyte quality and embryo development. Not only uterine but also non genital inflammatory sites can depreciate reproductive performances, with a carry over effect of 2 to 4 months. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234060PMC
October 2019

Developments of reproductive management and biotechnology in the pig.

Anim Reprod 2019 Oct 23;16(3):524-538. Epub 2019 Oct 23.

Department Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Finland.

This review aims to describe changes in production environment, management tools and technology to alleviate problems seen with the present hyperprolific sow model. Successful parturition in the pig includes the possibility to express adequate maternal behaviour, rapid expulsion of piglets, complete expulsion of placenta, elimination of uterine contamination and debris, neonatal activity and colostrum intake. We focus on management of large litters, including maternal behaviour, ease of parturition, colostrum production, piglet quality parameters and intermittent suckling. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234181PMC
October 2019

Oocytes, embryos and pluripotent stem cells from a biomedical perspective.

Anim Reprod 2019 Oct 23;16(3):508-523. Epub 2019 Oct 23.

Department of Veterinary and Animal Sciences, University of Copenhagen, Denmark.

The veterinary and animal science professions are rapidly developing and their inherent and historical connection to agriculture is challenged by more biomedical and medical directions of research. While some consider this development as a risk of losing identity, it may also be seen as an opportunity for developing further and more sophisticated competences that may ultimately feed back to veterinary and animal science in a synergistic way. The present review describes how agriculture-related studies on bovine embryo production through studies of putative bovine and porcine embryonic stem cells led the way to more sophisticated studies of human induced pluripotent stem cells (iPSCs) using e. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234146PMC
October 2019

Reproductive physiology of the heat-stressed dairy cow: implications for fertility and assisted reproduction.

Authors:
Peter J Hansen

Anim Reprod 2019 Oct 23;16(3):497-507. Epub 2019 Oct 23.

Department of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida, USA.

Heat stress causes a large decline in pregnancy success per insemination during warm times of the year. Improvements in fertility are possible by exploiting knowledge about how heat stress affects the reproductive process. The oocyte can be damaged by heat stress at the earliest stages of folliculogenesis and remains sensitive to heat stress in the peri-ovulatory period. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234026PMC
October 2019

Intrafollicular barriers and cellular interactions during ovarian follicle development.

Anim Reprod 2019 Oct 23;16(3):485-496. Epub 2019 Oct 23.

Faculty of Animal Sciences and Food Engineering, Department of Veterinary Medicine, University of São Paulo, Pirassununga, São Paulo, Brazil.

Follicles are composed of different interdependent cell types including oocytes, cumulus, granulosa, and theca cells. Follicular cells and oocytes exchange signaling molecules from the beginning of the development of the primordial follicles until the moment of ovulation. The follicular structure transforms during folliculogenesis; barriers form between the germ and the somatic follicular cells, and between the somatic follicular cells. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234062PMC
October 2019

New tools for cell reprogramming and conversion: Possible applications to livestock.

Anim Reprod 2019 Oct 23;16(3):475-484. Epub 2019 Oct 23.

Department of Health, Animal Science and Food Safety, University of Milan, Italy.

Somatic cell nuclear transfer and iPS are both forms of radical cell reprogramming able to transform a fully differentiated cell type into a totipotent or pluripotent cell. Both processes, however, are hampered by low efficiency and, in the case of iPS, the application to livestock species is uncertain. Epigenetic manipulation has recently emerged as an efficient and robust alternative method for cell reprogramming. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234139PMC
October 2019

DNA methylation, environmental exposures and early embryo development.

Anim Reprod 2019 Oct 23;16(3):465-474. Epub 2019 Oct 23.

Department of Biochemistry and Molecular Medicine, Université de Montréal, Research Center of the CHU Sainte-Justine. Montreal, Canada.

The first crucial step in the developmental program occurs during pre-implantation, the time after the oocyte has been fertilized and before the embryo implants in the uterus. This period represents a vulnerable window as the epigenome undergoes dynamic changes in DNA methylation profiles. Alterations in the early embryonic reprogramming wave can impair DNA methylation patterns and induce permanent changes to the developmental program, leading to the onset of adverse health outcomes in offspring. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234019PMC
October 2019

Stress, strain, and pregnancy outcome in postpartum cows.

Authors:
Matthew C Lucy

Anim Reprod 2019 Oct 23;16(3):455-464. Epub 2019 Oct 23.

Division of Animal Sciences, University of Missouri, Animal Science Research Center, Columbia, MO, USA.

Stress affects the productivity and fertility of cattle. Stress causes strain and individual animals experience different amounts of strain in response to the same amount of stress. The amount of strain determines the impact of stress on fertility. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234163PMC
October 2019

Folliculogenesis and acquisition of oocyte competence in cows.

Anim Reprod 2019 Oct 23;16(3):449-454. Epub 2019 Oct 23.

Département des Sciences Animales, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, Québec, Canada.

IVF success depends on hundreds of factors and details but the oocyte quality remains the most important and problematic issue. All antral follicles contain oocytes and all of them have that have reached their full size, can be aspirated, can mature and can be fertilized in vitro. But only a few will make it to embryo unless harvested at a very specific time/status. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234107PMC
October 2019

Contribution of the immune system to follicle differentiation, ovulation and early corpus luteum formation.

Anim Reprod 2019 Oct 23;16(3):440-448. Epub 2019 Oct 23.

School of Agriculture & Food Sciences, University College Dublin, Belfield, Dublin 4, Ireland.

Much of what we know about the involvement of the immune system in periovulatory follicle differentiation, ovulation and subsequent formation of the corpus luteum in cattle is drawn from the findings of studies in several mammalian livestock species. By integrating published histological data from cattle, sheep and pigs and referring back to the more comprehensive knowledge bank that exists for mouse and humans we can sketch out the key cells of the immune system and the cytokines and growth factors that they produce that are involved in follicle differentiation and luteinization, ovulation and early follicle development. These contributions are reviewed and the key findings, discussed. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234072PMC
October 2019

Embryo competence and cryosurvival: Molecular and cellular features.

Anim Reprod 2019 Oct 23;16(3):423-439. Epub 2019 Oct 23.

Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brasil.

Global cattle genetic market is experiencing a change of strategy, large genetic companies, traditionally recognized in the artificial insemination field, have also begun to operate in the embryo market. Consequently, the demand for produced (IVP) embryos has grown. However, the overall efficiency of the biotechnology process remains low. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234140PMC
October 2019

Strategies to increment and embryo production and transfer in cattle.

Anim Reprod 2019 Oct 23;16(3):411-422. Epub 2019 Oct 23.

Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.

Knowledge of follicular wave dynamics obtained through the use of real-time ultrasonography and the development of the means by which follicular wave dynamics can be controlled have provided practical approaches for the and production and transfer of embryos in cattle. The elective control of follicular wave emergence and ovulation has had a great impact on the application of on-farm embryo transfer, especially when large groups of donors need to be superstimulated at the same time. Although estradiol and progestins have been used for many years, practitioners in countries where estradiol cannot be used have turned to alternative treatments, such as mechanical follicle ablation or the administration of GnRH for the synchronization of follicle wave emergence. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234104PMC
October 2019

Pre-TAI protocol strategies to increase reproductive efficiency in beef and dairy cows.

Anim Reprod 2019 Oct 23;16(3):402-410. Epub 2019 Oct 23.

Ouro Fino Saúde Animal, Ribeirão Preto, SP, Brazil.

Ovulation synchronization protocols are well established in beef and dairy cows. However, the protocol response rate is around 70-90%. In beef cows, factors such as inadequate nutrition and calf presence negatively impact the response of progesterone (P4)/estradiol-based ovulation synchronization protocols by interfering with GnRH release and consequently reducing LH pulsatility and final follicular development. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234102PMC
October 2019

Intensive use of IVF by large-scale dairy programs.

Anim Reprod 2019 Oct 23;16(3):394-401. Epub 2019 Oct 23.

Universidade Estadual de Londrina, Laboratório de Reprodução Animal, DCV-CCA-UEL, Londrina, Parana, Brazil.

The number of embryos produced by fertilization (IVF) has grown exponentially in recent years. Recently, for the first time, the number of embryos produced and transferred was significantly higher than the number developed worldwide. In this context, a particular boost occurred with ovum pick-up (OPU) and embryos produced in North America, and this technology is becoming more prominent for commercial dairy farms. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234020PMC
October 2019

Sanitary program to reduce embryonic mortality associated with infectious diseases in cattle.

Anim Reprod 2019 Oct 22;16(3):386-393. Epub 2019 Oct 22.

National Institute of Science and Technology of Dairy Production Chain (INCT-Leite), Universidade Estadual de Londrina, Paraná, Brazil.

Among reproductive disorders in dairy and beef cattle worldwide, embryonic mortalities stand out as one of the most frequent. Because of the multifactorial etiology, the clinical and laboratory diagnoses of embryonic mortality causes in cattle are quite complex. Often, infectious causes may account for up to 50% of bovine embryonic mortality rates after 30 days of conception. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234040PMC
October 2019

Aspects of embryo-maternal communication in establishment of pregnancy in cattle.

Anim Reprod 2019 Oct 22;16(3):376-385. Epub 2019 Oct 22.

School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.

Establishment of pregnancy in mammals requires reciprocal molecular communication between the conceptus and endometrium that modifies the endometrial transcriptome and uterine luminal milieu to support pregnancy. Due to the small size of the early embryo and elongating conceptus relative to the volume of the uterine lumen, collection of endometrium adjacent to the developing conceptus is difficult following conventional uterine flushing methods in cattle. Use of endometrial explants in culture can overcome this challenge and reveal information about the dialogue between the developing embryo and the uterus. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234086PMC
October 2019

Challenges to increase the AI and ET markets in Brazil.

Anim Reprod 2019 Oct 22;16(3):364-375. Epub 2019 Oct 22.

Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.

Artificial insemination (AI) and embryo transfer (ET) are the most widely used biotechnologies in the world with the goal of increasing genetic gain and improving reproductive efficiency of beef and dairy herds. The protocols for ovulation synchronization for timed AI (TAI) or ET (TET) are tools that allow artificial insemination or transfer of a high number of embryos in a pre-established moment and without the necessity of estrous detection. Currently, 86% of inseminations in Brazil are performed using TAI (13. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7234119PMC
October 2019

Lectin spatial immunolocalization during capacitation in spermatozoa.

Anim Reprod 2020 Feb 5;17(1):e20190083. Epub 2020 Feb 5.

Departamento de Biotecnología, Universidad de Alicante, Alicante, España.

Spermatozoa interactions with the female reproductive tract and oocyte are regulated by surface molecules such as glycocalyx. The capacitation process comprises molecular and structural modifications which increase binding affinity. Lectins allowed us to describe glycocalyx changes during maturation, capacitation and acrosome reaction. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216573PMC
February 2020

Current and alternative trends in antibacterial agents used in mammalian semen technology.

Anim Reprod 2020 Mar 17;17(1):e20190111. Epub 2020 Mar 17.

Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.

The use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212743PMC

Change in plasma sirtuin 1 level by injection into uterus of resveratrol in Korean cattle.

Anim Reprod 2020 Mar 17;17(1):e20190090. Epub 2020 Mar 17.

Department of Biotechnology, College of Agriculture & Life Science, Hankyong National University, Gyeonggi, Republic of Korea.

Bovine embryonic development is closely associated with mitochondrial biogenesis, which is regulated by the sirtuin 1 (SIRT1). Resveratrol, which is a type of natural phenol produced by several plants and used as a dietary supplement, is the activator of SIRT1. Although it has been reported that resveratrol increased SIRT1 level in bovine blastocysts, there are no reports on the change in the plasma SIRT1 level in cows. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212746PMC

Effects of the cryopreservation process on dog sperm integrity.

Anim Reprod 2020 Mar 24;17(1):e20190081. Epub 2020 Mar 24.

Departamento de Cirurgia Veterinária e Reprodução Animal, Universidade Estadual Paulista "Julio de Mesquita Filho", Botucatu, SP, Brasil.

Sperm cryopreservation has become an indispensable tool in reproductive biology. However, frozen/thawed semen has a short lifespan due to loss of sperm cell integrity. To better understand which sperm cell structures are compromised by the cryopreservation process and apoptosis markers, the sperm of five healthy mature dogs was analyzed in this study. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212748PMC

Wild induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract.

Anim Reprod 2020 Feb 20;17(1):e20190078. Epub 2020 Feb 20.

Centro de Aquicultura, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.

Breeding technology is of utmost importance for reproduction of wild fish in captivity for the reintroduction and selective breeding programs purposes. The main challenge is that when applied to wild undomesticated specimens, conventional protocols often cause breeders and/or embryo mortality and spawning failure. In this study, we evaluated the reproductive performance of wild , a great importance fish for subsistence fishing in South American rivers, applying conventional and lower-dose hormonal therapies by means of two consecutive experiments. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212741PMC
February 2020

Effect of temperature - humidity index on the onset of post- partum ovarian activity and reproductive behavior in cows.

Anim Reprod 2020 Feb 20;17(1):e20190074. Epub 2020 Feb 20.

Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México.

The effect of climatic factors on ovarian activity and reproductive behavior (RB) was evaluated in 46 cows kept under grazing conditions. Temperature-humidity index (THI) was used as an indicator of stress and divided in alert, damage and emergency levels. Fat thickness (FAT) was taken during the last trimester of gestation (LTG) to approximately 90d postpartum (PP). Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212737PMC
February 2020

The gynecological examination in Pantaneiro mares.

Anim Reprod 2020 Mar 17;17(1):e20190031. Epub 2020 Mar 17.

Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.

Gynecological examination is essential to assess the reproductive tract of mares and can provide important information about the uterine environment. It includes physical, vaginal, and rectal examination, ultrasound, cytology, culture, and endometrial biopsy. The present study aimed to perform gynecological examination and fertility to assess the fertility prognosis of Pantaneiro mares that have not been reproductively active and to determine their reproductive ability. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212739PMC

Reproductive performance of sows inseminated with semen doses stored for up to seven days in long-term extender in a field condition.

Anim Reprod 2020 Feb 11;17(1):e20190121. Epub 2020 Feb 11.

Magapor S.L., Zaragoza, España.

This study aimed to evaluate the reproductive performance of sows inseminated with semen doses preserved at 15-18 °C for up to seven days in long-term extender (Duragen). Parity one (PO1) to PO7 sows were randomly assigned to the following groups: AI1-3 (n=190), insemination with semen doses stored between one and three days; and AI5-7 (n=124), insemination with semen doses stored between five and seven days. Sows were submitted to estrus detection twice a day. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189442PMC
February 2020

Testicular development induced by GnRH-IS in budgerigar ().

Anim Reprod 2020 Feb 11;17(1):e20190103. Epub 2020 Feb 11.

Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana Unidad Xochimilco, México.

Nowadays, the third part of parrots in the world is endangered or vulnerable; an alternative for their preservation is assisted reproduction in captivity through hormonal manipulation. In birds, GnRH is the main hormone which controls reproductive physiology, it is known there are three types: GnRH-I, GnRH-II and GnRH-III, involved in the release or inhibition of luteinizing hormone and follicle stimulant hormone to control gonadal and gametic development. The objective of this study was, to evaluate the effect of administrating synthetic GnRH-I in the testicular development of . Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189523PMC
February 2020

Effect of supplementation of extracts in cold storage media and cryopreservation of domestic cat epididymal spermatozoa.

Anim Reprod 2020 Jan 30;17(1):e20190067. Epub 2020 Jan 30.

Universidade Federal Rural do Semi-Árido - UFERSA - Mossoró, RN, Brasil.

This study evaluated the effect of the extract of at concentrations of 10% and 20% on the cryopreservation of sperm from the epididymis of domestic cats. Epididymal spermatozoa were recovered using the flotation technique and used in the treatments: control (TRIS-egg yolk at 20%), T10% (TRIS plus 10% of extract), and T20% (TRIS plus 20% of extract). The spermatozoa were subjected to 4ºC for 60 minutes, followed by 20 minutes in nitrogen vapors, and stored in a cryogenic cylinder. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189537PMC
January 2020

Subclinical endometritis and postpartum ovarian resumption in respect to TNF-α, IL-8 and CRP in Egyptian buffaloes.

Anim Reprod 2020 Jan 27;17(1):e20190027. Epub 2020 Jan 27.

Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

The study was carried out to find the relation between subclinical endometritis (SCE) and postpartum (pp.) ovarian resumption as well as to evaluate serum and endometrial TNF-α, IL-8 and serum CRP in buffaloes with and without SCE. Thirty-nine pluriparous buffaloes at the 3 (W3), 5 (W5) and 7 (W7) week pp. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190313PMC
January 2020

Relationship between Body Mass Index (BMI) and testicular and hormonal parameters of sexually active male greater cane rats ().

Anim Reprod 2020 Feb 5;17(1):e20190026. Epub 2020 Feb 5.

Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.

The current upsurge in intensive farming practices of greater cane rat has not only lead to higher growth rate but is accompanied by increased fat deposition especially in the males. This study attempts to characterize one of the most commonly used fat estimation parameter, the body mass index (BMI) as well as evaluates its relationship with testicular and hormonal parameters in seventy-two sexually active male cane rats over a period of one year. Six animals, kidded and raised in a farm, with known ages were used each month. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189494PMC
February 2020

Chlorogenic acid improves the quality of boar semen processed in Percoll.

Anim Reprod 2020 Jan 24;17(1):e20190021. Epub 2020 Jan 24.

Departamento de Medicina Veterinária, Universidade Federal de Lavras, Lavras, MG, Brasil.

This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control - vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189441PMC
January 2020

Inhibition of Na, K -ATPase with ouabain is detrimental to equine blastocysts.

Anim Reprod 2020 Jan 22;17(1). Epub 2020 Jan 22.

Departamento de Medicina Veterinária, Laboratório de Reprodução Animal, Universidade Federal de Alagoas, Viçosa, AL, Brasil.

Although equine blastocysts ≤ 300 µm in diameter can be successfully vitrified, larger equine blastocysts are not good candidates for cryopreservation. As Na, K-ATPase is involved in maintaining blastocyst expansion, perhaps inhibition of this enzyme would be a viable method of reducing blastocyst diameter prior to cryopreservation. Objectives were to evaluate effects of ouabain-induced inhibition of Na, K-ATPase in equine blastocysts. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189547PMC
January 2020

Association of L-arginine with heparin on the sperm capacitation improves embryo production in bovine.

Anim Reprod 2019 Nov 18;16(4):938-944. Epub 2019 Nov 18.

Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Laboratório de Engenharia Agrícola, Campos dos Goytacazes, RJ, Brasil.

We aimed to evaluate the effects of L-arginine (L-arg) in the quality of heparin-induced capacitation of cryopreserved bovine spermatozoa and its effects on IVP. The experimental groups were: Control 0 hour without pre-capacitation, and groups of sperm capacitated for 30 min in the absence of COC with heparin (Control 30 min), with 1 mM L-arg and with 1 mM L-arg + heparin. The capacitation pattern was evaluated by chlortetracycline assay and the integrity of the plasma membrane (PM) and acrosome membrane (AM) by the association of Hoescht 33342 and propidium iodide. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189474PMC
November 2019

Detection of subsp. in reproductive tissue and semen of naturally infected rams.

Anim Reprod 2019 Nov 18;16(4):930-937. Epub 2019 Nov 18.

Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.

subsp. (MAP) is the causative agent of paratuberculosis (PTB), disease that causes a syndrome of bad nutrient absorption, weight loss and eventually death. The intestine is the main target organ where the infection develops; however, there is evidence of infection by MAP in extra-intestine sites of sheep, including mesenteric nodes and semen. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189526PMC
November 2019

Relationship between fetal biometric assessment by ultrasonography and neonatal lamb vitality, birth weight and growth.

Anim Reprod 2019 Nov 18;16(4):923-929. Epub 2019 Nov 18.

Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP, Brasil.

Ultrasonographic examination of pregnant ewes can enable the identification of perinatal abnormalities and establish prenatal assistance responsible for minimizing morbidity and perinatal mortality. Therefore, we aimed to evaluate the feasibility of a fetal biometric analysis by ultrasonography to predict neonatal vitality and lamb growth during the first month of life. A longitudinal study was conducted with 13 healthy ewes, subjected to ultrasonographic examination every 15 days from 60 day of pregnancy until lambing, evaluating thoracic diameter, abdominal diameter, biparietal diameter, humerus, femur and placentome length. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189444PMC
November 2019

Effect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cows.

Anim Reprod 2019 Nov 18;16(4):914-922. Epub 2019 Nov 18.

Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.

The aim of this study was to evaluate the long-term effects of recombinant bovine somatotropin (rbST) on follicle population and ovulatory follicle development in non-lactating dairy cows. Twenty-one Jersey cows were allocated in rbST (n=11) or control (n=10) groups. On day -60, cows in rbST group received 500 mg of somatotropin (s. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189501PMC
November 2019

Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( ).

Anim Reprod 2019 Nov 18;16(4):902-913. Epub 2019 Nov 18.

Universidade Estadual Vale do Acaraú Laboratorio de Biologia Molecular Sobral Brasil Universidade Estadual Vale do Acaraú, Laboratorio de Biologia Molecular, Sobral, Brasil.

The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed's genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189512PMC
November 2019

Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal.

Anim Reprod 2019 Nov 18;16(4):895-901. Epub 2019 Nov 18.

Universidade Federal Rural de Pernambuco Laboratório de Andrologia Departamento de Medicina Veterinária RecifePE Brasil Universidade Federal Rural de Pernambuco, Laboratório de Andrologia, Departamento de Medicina Veterinária, Recife, PE, Brasil.

The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189563PMC
November 2019

Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C.

Anim Reprod 2019 Nov 14;16(4):887-894. Epub 2019 Nov 14.

Universidad de Zaragoza, Facultad de Veterinaria, Área de Obstetricia y Reproducción Animal, Departamento de Patología Animal, España.

Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl-2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4ºC and 16ºC. Sperm samples were diluted with INRA 96 (Extender A), INRA 96 with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4ºC and 16ºC. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189548PMC
November 2019

Testicular needle aspiration: Is it a safe method for breeding soundness evaluation in bulls?

Anim Reprod 2019 Nov 18;16(4):880-886. Epub 2019 Nov 18.

Universidade Santo Amaro, Programa de Pós-graduação em Medicina Veterinária e Bem-Estar Animal, São Paulo, SP, Brasil.

The aim of this study was to evaluate the impact of successive bovine testicular punctures using different needle sizes. Fifteen bulls were submitted to testicular needle aspiration (TNA) in the left and right testis using 18-gauge (40×12mm) or 22-gauge (25×7mm) needles, respectively, once every 30 days. Animals were randomly divided into three groups, which were submitted to bilateral orchiectomy two days after the last puncture. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189565PMC
November 2019

Effect of bacterial endotoxin lipopolysaccharide treatment on duck Leydig cells.

Anim Reprod 2019 Nov 18;16(4):871-879. Epub 2019 Nov 18.

Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, China.

This study aimed to investigate the effects of bacterial endotoxin lipopolysaccharide (LPS) on hormone production and gene expression in duck Leydig cells and its underlying mechanisms. Leydig cells were collected from 200-day-old mallard ducks and divided into five treatment groups (0, 50, 100, 200, and 400 ng/mL LPS). After treatment with LPS for 6, 12, 24, and 48 h, testosterone, activin, and inhibin levels in the cell supernatants were determined using enzyme-linked immunosorbent assay (ELISA) kits. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189506PMC
November 2019

Storage of boar semen at 16-18 °C in the long-term commercial extender prepared with deionized water or nanowater.

Anim Reprod 2019 Nov 18;16(4):864-870. Epub 2019 Nov 18.

University of Guelph, Ontario Veterinary College, Department of Biomedical Sciences, Guelph, Canada.

The aim of this study was to assess the usefulness of nanowater (NW; water declusterized using cold plasma treatment) as a diluent for a commercial boar semen extender during the 15-day storage (Days 1 to 15) at 16-18 °C. Ejaculates collected from 8 boars were subjected to the standard evaluation and then diluted in the extender prepared with deionized water (DW) or NW to a final concentration of 3×10 spermatozoa/ml. The proportion of defective spermatozoa increased (P<0. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189532PMC
November 2019

Effect of live body weight and method of synchronization on ovulation, pregnancy rate and embryo and fetal loss in buffalo heifers.

Anim Reprod 2019 Nov 18;16(4):859-863. Epub 2019 Nov 18.

Università degli Studi di Napoli "Federico II", Dipartimento di Medicina Veterinaria e Produzioni Animali, Napoli, Italy.

This study aimed to assess the influence of live body weight (LBW) and age on reproductive performance in buffalo heifers synchronized by different treatments. The study was carried out on 146 Mediterranean buffalo heifers (mean age 25.3±13. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189557PMC
November 2019

Echotextural characteristics of the mammary gland during early lactation in two breeds of sheep varying in milk yields.

Anim Reprod 2019 Nov 18;16(4):853-858. Epub 2019 Nov 18.

University of Guelph Ontario Veterinary College Department of Biomedical Sciences Guelph Canada University of Guelph, Ontario Veterinary College, Department of Biomedical Sciences, Guelph, Canada.

The main goal of this preliminary study was to determine and compare ultrasonographic characteristics of the mammary gland in two genotypes of ewes varying in milk productivity at 2, 3 and 4 weeks after lambing. Ultrasonographic images of the udder were obtained using the 5.0- and 7. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189527PMC
November 2019

Association between gestation length and lactation performance, lactation curve, calf birth weight and dystocia in Holstein dairy cows in Iran.

Anim Reprod 2019 Nov 18;16(4):846-852. Epub 2019 Nov 18.

Shiraz University School of Veterinary Medicine Department of Clinical Science Shiraz Iran Shiraz University, School of Veterinary Medicine, Department of Clinical Science, Shiraz, Iran.

In this study, 252,798 lactations on 108,077 cows in 433 herds were used to determine the association between gestation length (GL) and lactation performance, lactation curve, calf birth weight and dystocia in Holstein dairy cows in Iran. The GL averaged 278.1 ± 5. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189531PMC
November 2019

Powdered coconut water (ACP 406®) as an alternative base culture medium for culture of goat preantral follicles enclosed in ovarian tissue.

Anim Reprod 2019 Nov 18;16(4):838-845. Epub 2019 Nov 18.

Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos Inclusos em Folículos Ovarianos Pré-antrais, Fortaleza, CE, Brasil.

This study evaluated a powdered coconut water solution (ACP 406®) as a base culture medium on the survival and development of goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 100 μg/mL penicillin and 100 μg/mL streptomycin) were evaluated: α-MEM (α-MEM alone, without additional supplementation); α-MEM+ (supplemented α-MEM); ACP (ACP®406 alone); or ACP+ (supplemented ACP®406). Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189440PMC
November 2019

Transforming growth factor-beta family members are regulated during induced luteolysis in cattle.

Anim Reprod 2019 Nov 18;16(4):829-837. Epub 2019 Nov 18.

Universidade Federal de Pelotas, Departamento de Patologia Animal, Capão do Leão, RS, Brasil.

The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis . Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189511PMC
November 2019

Supplemented powdered coconut water (ACP-406) promotes growth of goat secondary follicles and oocyte meiotic resumption.

Anim Reprod 2019 Nov 18;16(4):819-828. Epub 2019 Nov 18.

Universidade Federal do Vale do São Francisco, Núcleo de Biotecnologia Aplicada ao Desenvolvimento de Folículos Ovarianos, Petrolina, PE, Brasil.

The objective of this study was to test the efficiency of powdered coconut water (ACP-406) base-medium without or with the addition of supplements on culture of isolated goat secondary follicles. Follicles were cultured for 18 days in α-MEM or in ACP-406, both without supplements (referred to as α-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as α-MEM and ACP). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after maturation were evaluated. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2019-0008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189492PMC
November 2019

Effect of metronidazole on placental and fetal development in albino rats.

Anim Reprod 2019 Nov 18;16(4):810-818. Epub 2019 Nov 18.

Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, PE, Brasil.

Metronidazole is an antiprotozoal and antibacterial used in gynecology and obstetrics for the treatment of parasitic infections. However, despite having clinical use for more than three decades, questions about the safety of its use during pregnancy is not well understood. Thus, the present study evaluated the effect of metronidazole on placental and fetal development in pregnant rats. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189510PMC
November 2019

Technical recommendations for artificial insemination in sheep.

Anim Reprod 2019 Nov 18;16(4):803-809. Epub 2019 Nov 18.

Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Bariloche, Grupo de Reproducción en Rumiantes Menores, Bariloche, Argentina.

Artificial insemination (AI) was the first important biotechnology applied to improve the genetics of farm animals. It allows the rapid and massive diffusion of desirable characteristics of males with high productive potential. We describe the different types of estrus induction and synchronization techniques and the use of the AI with fresh, chilled or frozen semen. Read More

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http://dx.doi.org/10.21451/1984-3143-AR2018-0129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189475PMC
November 2019

Regulation of germ line stem cell homeostasis.

Anim Reprod 2015 Jan-Mar;12(1):35-45

Department of Endocrine Neoplasia and Hormonal Disorders, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Mammalian spermatogenesis is a complex process in which spermatogonial stem cells of the testis (SSCs) develop to ultimately form spermatozoa. In the seminiferous epithelium, SSCs self-renew to maintain the pool of stem cells throughout life, or they differentiate to generate a large number of germ cells. A balance between SSC self-renewal and differentiation is therefore essential to maintain normal spermatogenesis and fertility. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341791PMC
March 2017
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