7,339,547 results match your criteria Animal Nutrition and Feed Technology[Journal]

Immune determinants of endometrial receptivity: a biological perspective.

Fertil Steril 2022 Jun;117(6):1107-1120

Robinson Research Institute and Adelaide Medical School, The University of Adelaide, Adelaide, South Australia, Australia.

Immune cells are essential for endometrial receptivity to embryo implantation and early placental development. They exert tissue-remodeling and immune regulatory roles-acting to promote epithelial attachment competence, regulate the differentiation of decidual cells, remodel the uterine vasculature, control and resolve inflammatory activation, and suppress destructive immunity to paternally inherited alloantigens. From a biological perspective, the endometrial immune response exerts a form of "quality control"-it promotes implantation success when conditions are favorable but constrains receptivity when physiological circumstances are not ideal. Read More

View Article and Full-Text PDF

Disruption of Circadian Rhythms by Shift Work Exacerbates Reperfusion Injury in Myocardial Infarction.

J Am Coll Cardiol 2022 May;79(21):2097-2115

State Key Laboratory for Oncogenes and Related Genes, Division of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Cancer Institute, Shanghai, China. Electronic address:

Background: Shift work is associated with increased risk of acute myocardial infarction (AMI) and worsened prognosis. However, the mechanisms linking shift work and worsened prognosis in AMI remain unclear.

Objectives: This study sought to investigate the impact of shift work on reperfusion injury, a major determinant of clinical outcomes in AMI. Read More

View Article and Full-Text PDF

A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis.

J Vet Sci 2022 May;23(3):e50

College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.

Background: There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity. Read More

View Article and Full-Text PDF

Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular small colony variants.

J Vet Sci 2022 May;23(3):e48

Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control, College of Animal Science, Tarim University, Alar 843300, China.

Background: The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by small colony variants (SASCVs).

Objectives: In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.

Methods: Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes. Read More

View Article and Full-Text PDF

Impact of inland waters on highly pathogenic avian influenza outbreaks in neighboring poultry farms in South Korea.

J Vet Sci 2022 May;23(3):e36

Department of Veterinary Internal Medicine, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Korea.

Background: Since 2003, the H5 highly pathogenic avian influenza (HPAI) subtype has caused massive economic losses in the poultry industry in South Korea. The role of inland water bodies in avian influenza (AI) outbreaks has not been investigated. Identifying water bodies that facilitate risk pathways leading to the incursion of the HPAI virus (HPAIV) into poultry farms is essential for implementing specific precautionary measures to prevent viral transmission. Read More

View Article and Full-Text PDF

Transcriptome analysis of CRISPR/Cas9-mediated GPAM in bovine mammary epithelial cell-line unravelled the effects of GPAM gene on lipid metabolism.

Gene 2022 May 23:146574. Epub 2022 May 23.

College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, P.R.China. Electronic address:

Glycerol-3-phosphate acyltransferase mitochondrial (GPAM) is an enzyme in animal lipid metabolism pathways that catalyzes the initial and most committed step of glycerolipid biosynthesis. The present study mainly focused on exploring the relationship between the GPAM gene and the lipid metabolism of mammary epithelial cells and the effect of GPAM on the related pathways of lipid metabolism. The GPAM gene was knocked out entirely in bovine mammary epithelial cells(BMECs) using CRISPR/Cas9 technology, and the mechanism by which the GPAM gene regulates lipid metabolism in BMECs was confirmed. Read More

View Article and Full-Text PDF

Using comparative genomics to detect mutations regulating plumage variations in graylag (A. anser) and swan geese (A. cygnoides).

Gene 2022 May 23:146612. Epub 2022 May 23.

Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, P.R. China. Electronic address:

Although graylag geese (A. anser) showed similar plumages of white, grey, and white with grey patches compared to those in swan geese (A. cygnoides), it was believed the substantial molecular mechanism for plumage variations were different. Read More

View Article and Full-Text PDF

Cancer germline antigen gene MAGEB2 promotes cell invasion and correlates with immune microenvironment and immunotherapeutic efficiency in laryngeal cancer.

Clin Immunol 2022 May 23:109045. Epub 2022 May 23.

Department of Head and Neck Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen 518116, Guangdong Province, PR China. Electronic address:

By multiple transcriptome datasets (TCGA, GSE59102, GSE25727, GSE27020 and GSE65858) and multi-omics (RNA-seq, SNP, CNV, DNA methylation) in-depth analysis, we found that cancer germline antigen (CGA) family/genes MAGEB2 is involved in the imitation, progression and prognosis in LC as well as correlated positively with lymphatic metastasis and negatively with DNA methylation. Then, in vitro experiment verified that MAGEB2 expression renders significant alteration in LC tissues and cells via immunohistochemical (IHC), qRT-PCR and western blot (WB), and up-regulation of MAGEB2 expression could facilitate the proliferation, migration and invasion of LC cells and vice versa. Subsequently, MAGEB2 knockdown suppressed tumor growth and lung metastasis in vivo animal experiment, while MAGEB2 overexpression promoted tumor growth and lung metastasis. Read More

View Article and Full-Text PDF

Ser392 phosphorylation modulated a switch between p53 and transcriptional condensates.

Biochim Biophys Acta Gene Regul Mech 2022 May 23:194827. Epub 2022 May 23.

Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong Province 510006, PR China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China. Electronic address:

Human p53 is a transcription factor regulating the transcription of a variety of target genes. Under various stresses, its tumor suppressor function was activated by the phosphorylation of p53. In this study, we found that full-length wild-type p53 could form phase-separated condensates with the aggregation tendency in vitro and in vivo. Read More

View Article and Full-Text PDF

Effects of biochar and biogas residue amendments on NO emission, enzyme activities and functional genes related with nitrification and denitrification during rice straw composting.

Bioresour Technol 2022 May 23:127359. Epub 2022 May 23.

State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410029, China.

This study was carried out to determine the response characteristics of NO emission, enzyme activities, and functional gene abundances involved in nitrification/denitirification process with biochar and biogas residue amendments during rice straw composting. The results revealed that NO release mainly occurred during the second fermentation phase. Biogas residue amendment promoted NO emission, while biochar addition decreased its emission by 33. Read More

View Article and Full-Text PDF

Effects of the Clostridium butyricum on growth performance, antioxidant capacity, immunity and disease resistance of Litopenaeus Vannamei fed with cottonseed protein concentrate (CPC) replacement of fishmeal in diet.

Fish Shellfish Immunol 2022 May 23. Epub 2022 May 23.

Guangdong Evergreen Feed Industry Co.Ltd, Zhanjiang, 524088, PR China.

Clostridium butyricum (CB) is a gram-positive bacterium that secretes short-chain fatty acids such as butyric acid and so on. An 8-week feeding trial was conducted to investigate the effects of CB on the growth performance, antioxidant capacity, immunity and resistance to Vibrio parahaemolyticus in Litopenaeus Vannamei fed with cottonseed protein concentrate (CPC) replacement of fishmeal. Six iso-nitrogenous (40%) and iso-lipidic (6%) diets were formulated including a positive control group (PC, 25% fishmeal), a negative control group (NC, CPC replaced 30% of fishmeal protein), and 0. Read More

View Article and Full-Text PDF

A novel behavioral paradigm to measure anxiety-like behaviors in zebrafish by the concomitant assessment of geotaxis and scototaxis.

Prog Neuropsychopharmacol Biol Psychiatry 2022 May 23:110579. Epub 2022 May 23.

Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS 97105-900, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS 97105-900, Brazil; The International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA. Electronic address:

Pathological anxiety is a set of diseases characterized by specific clinical manifestations and the use of alternative models may provide novel insights in translational neurobehavioral research. In zebrafish, the separate performance of novel tank and light dark tests in different order to assess anxiety using same animals may provide conflicting data due to the battery effect and/or time-drug-response and variability across tests. To improve data reliability, we aimed to characterize a novel behavioral paradigm to measure geotaxis and scototaxis as anxiety-like responses in the same trial. Read More

View Article and Full-Text PDF

Earthworm half-pipe assay: A new alternative in vivo skin corrosion test using invertebrates.

Environ Pollut 2022 May 23:119519. Epub 2022 May 23.

Department of Environmental Health Science, Konkuk University, Seoul, 05029, Republic of Korea. Electronic address:

As a result of the efforts to introduce the principle of the 3Rs (replacement, reduction, and refinement) into animal testing, alternative in vitro skin corrosion test methods have been developed and standardized globally. However, alternative in vitro skin corrosion test methods have some limitations in terms of the use of humanely killed rats or commercial models and kits. The present study focused on the applicability of invertebrates as alternative in vivo skin models. Read More

View Article and Full-Text PDF

Global landscape of gut microbiome diversity and antibiotic resistomes across vertebrates.

Sci Total Environ 2022 May 23:156178. Epub 2022 May 23.

CAS Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Center for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China. Electronic address:

Multiple factors influence gut microbiome diversity in vertebrate hosts. Most previous studies have only investigated specific factors and certain host species or taxa. However, a comprehensive assessment of the relative contributions of individual factors towards gut microbial diversity within a broader evolutionary context remains lacking. Read More

View Article and Full-Text PDF

Habitat loss predicts the functional extinction of fish from Amazonian streams during the Anthropocene.

Sci Total Environ 2022 May 23:156210. Epub 2022 May 23.

Programa de Pós-Graduação em Ecologia, Laboratório de Ecologia de Produtores, Instituto de Ciências Biológicas, Universidade Federal do Pará, Av. Perimetral, 2651 - Terra Firme, Belém, PA, 66077-530, Brazil; Universidade Federal Rural da Amazônia, Instituto Socioambiental e dos Recursos Hídricos, Avenida Perimetral, 660778-30 Belém, PA, Brazil.

The evaluation of extinction risk has typically focused on individual species, although a shift to a focus on ecosystem functioning would appear to be an urgent priority for conservation planning, especially considering that a sixth mass extinction event has already begun. In the present study, we investigated how fish extinction driven by habitat loss may modify the functioning of freshwater Amazonian ecosystems. We sampled the fish and environmental conditions of 63 streams in the eastern Amazon and simulated extinction based on the vulnerability of the species to habitat loss, which is the principal threat to tropical biodiversity. Read More

View Article and Full-Text PDF

Results of untargeted analysis using the SOMAscan proteomics platform indicates novel associations of circulating proteins with risk of progression to kidney failure in diabetes.

Kidney Int 2022 May 23. Epub 2022 May 23.

Section on Genetics and Epidemiology, Research Division, Joslin Diabetes Center, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA. Electronic address:

This study applies a large proteomics panel to search for new circulating biomarkers associated with progression to kidney failure in individuals with diabetic kidney disease. Four independent cohorts encompassing 754 individuals with type 1 and type 2 diabetes and early and late diabetic kidney disease were followed to ascertain progression to kidney failure. During ten years of follow-up, 227 of 754 individuals progressed to kidney failure. Read More

View Article and Full-Text PDF

Antiviral activity of brequinar against African swine fever virus infection in vitro.

Virus Res 2022 May 23:198826. Epub 2022 May 23.

Laboratory of Antiviral Drug Discovery, Institute of Molecular Biology of NAS, E. Hasratyan 7, 0014, Yerevan, Armenia; Denovo Sciences CJSC, H. Hakobyan 3, 0033, Yerevan, Armenia. Electronic address:

African swine fever virus (ASFV) is a double-stranded DNA virus that causes an acute and hemorrhagic disease in domestic swine, resulting in significant economic losses to the global porcine industry. The lack of vaccines and antiviral drugs highlights the urgent need for antiviral studies against ASFV. Here, we report that brequinar (BQR), which is a specific inhibitor of dihydroorotate dehydrogenase, robustly inhibits ASFV replication in Vero cells, as well as in porcine macrophages. Read More

View Article and Full-Text PDF

Ageing fundus degenerations of Macaca fascicularis on multi-modal imaging and histopathology: Similarities and differences compared to human.

Exp Eye Res 2022 May 23:109126. Epub 2022 May 23.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China. Electronic address:

To characterize the ageing fundus degenerations in Macaca fascicularis, we used multimodal imaging including color fundus photograph, spectral domain optical coherence tomography, fundus autofluorescence, fundus fluorescence angiography, and indocyanine green angiography (ICGA) to survey and track fundus changes of 84 Macaca fascicularis, ranging from 5 to 24 years old over 2 years, and followed by hematoxylin-eosin (HE) and immunofluorescence (IF) staining. The Macaca fascicularis in our cohort showed ageing characteristics different from human, including the more common yellow dot maculopathy, the unique appearance of patchy hyperautoflurescence, and the absence of subretinal drusenoid deposit, basal laminar deposit, geographic atrophy or choroidal neovascularization. Same with human, hard drusen, soft drusen, atherosclerosis, tessellated retina, staining of vessels in peripheral choroid on late-phase ICGA, and peripheral hard drusen were detected. Read More

View Article and Full-Text PDF

Maintaining the thyroid gland in mutant thyroglobulin-induced hypothyroidism requires thyroid cell proliferation that must continue in adulthood.

J Biol Chem 2022 May 23:102066. Epub 2022 May 23.

The Division of Metabolism, Endocrinology & Diabetes, University of Michigan Medical Center, Ann Arbor, MI 48105. Electronic address:

Congenital hypothyroidism with biallelic thyroglobulin mutation is an endoplasmic reticulum (ER) storage disease. Many patients (and animal models) grow an enlarged thyroid (goiter), yet some do not. In adulthood, hypothyroid TG mice (bearing a Tg-L2263P mutation) exhibit a large goiter, whereas adult WIC rats bearing the TG mutation (Tg-G2298R) exhibit a hypoplastic thyroid. Read More

View Article and Full-Text PDF

A drug-responsive multicellular human spheroid model to recapitulate drug-induced pulmonary fibrosis.

Biomed Mater 2022 May 26. Epub 2022 May 26.

Department of Bioengineering / EgeSAM-Ege University Translational Pulmonary Research Center, Ege University, Bornova, Izmir, 35040, TURKEY.

Associated with a high mortality rate, pulmonary fibrosis (PF) is the end stage of several interstitial lung diseases. Although many factors are linked to PF progression, initiation of the fibrotic process remains to be studied. Current research focused on generating new strategies to gain a better understanding of the underlying disease mechanism as the animal models remain insufficient to reflect human physiology. Read More

View Article and Full-Text PDF

Long-term mechanical loading is required for the formation of 3D bioprinted functional osteocyte bone organoids.

Biofabrication 2022 May 26. Epub 2022 May 26.

ETH Zurich Department of Health Sciences and Technology, Leopold-Ruzicka-Weg 4, Zurich, Zürich, 8093, SWITZERLAND.

Mechanical loading has been shown to influence various osteogenic responses of bone-derived cells and bone formation in vivo. However, the influence of mechanical stimulation on the formation of bone organoid in vitro is not clearly understood. Here, 3D bioprinted human mesenchymal stem cells (hMSCs)-laden graphene oxide composite scaffolds were cultured in a novel cyclic-loading bioreactors for up to 56 days. Read More

View Article and Full-Text PDF

Profiles of antibiotic- and heavy metal-related resistance genes in animal manure revealed using a metagenomic analysis.

Ecotoxicol Environ Saf 2022 May 24;239:113655. Epub 2022 May 24.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China; Agro-environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China. Electronic address:

Farmed animals produce excrement containing excessive amounts of toxic heavy metals as a result of consuming compound feed as well as receiving medical treatments, and the presence of these heavy metals may aggravate the risk of spreading drug-resistance genes through co-selection during manure treatment and application processes. However, research on the association between heavy metals and antimicrobial resistance is still lacking. In this study, metagenomic sequencing was used to explore the effects of the co-selection of environmentally toxic heavy metals on the resistome in manure. Read More

View Article and Full-Text PDF

Melatonin enhances the antioxidant capacity to rescue the honey bee Apis mellifera from the ecotoxicological effects caused by environmental imidacloprid.

Ecotoxicol Environ Saf 2022 May 24;239:113622. Epub 2022 May 24.

College of Life Sciences, Chongqing Normal University, Chongqing, China; Chongqing Key Laboratory of Microsporidia Infection and Control, China; The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Imidacloprid severely poisons the nontarget insect honey bee Apis mellifera. Few treatments are available to mitigate the adverse effects of imidacloprid. The primary concern is that the molecular understanding of imidacloprid toxicity is not comprehensive enough. Read More

View Article and Full-Text PDF

Complex model calibration through emulation, a worked example for a stochastic epidemic model.

Epidemics 2022 May 16;39:100574. Epub 2022 May 16.

School of Mathematics and Statistics, University of Glasgow, Glasgow, UK. Electronic address:

Uncertainty quantification is a formal paradigm of statistical estimation that aims to account for all uncertainties inherent in the modelling process of real-world complex systems. The methods are directly applicable to stochastic models in epidemiology, however they have thus far not been widely used in this context. In this paper, we provide a tutorial on uncertainty quantification of stochastic epidemic models, aiming to facilitate the use of the uncertainty quantification paradigm for practitioners with other complex stochastic simulators of applied systems. Read More

View Article and Full-Text PDF

Trans-anethole ameliorates LPS-induced inflammation via suppression of TLR4/NF-κB pathway in IEC-6 cells.

Int Immunopharmacol 2022 May 23;108:108872. Epub 2022 May 23.

College of Animal Sciences and Technology, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, China. Electronic address:

This study was undertaken to investigate the protective role of trans-anethole (TA) in lipopolysaccharide (LPS)-induced rat intestinal epithelial cells (IEC-6) injury and the potential mechanisms. The cells were pretreated with TA (0 and 1 mM) for 24 h, prior to stimulation by LPS (1 mg/mL) for 24 h. Compared with the control group (CON), LPS stimulus resulted in decreased cell viability, intestinal barrier injury, increased cell apoptosis and cell cycle arrest at the G2/M phase. Read More

View Article and Full-Text PDF

Heterologous ChAdOx1/BNT162b2 vaccination induces stronger immune response than homologous ChAdOx1 vaccination: The pragmatic, multi-center, three-arm, partially randomized HEVACC trial.

EBioMedicine 2022 May 23;80:104073. Epub 2022 May 23.

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, Peter-Mayr-Str. 4b, 6020 Innsbruck, Austria. Electronic address:

Background: Several COVID-19 vaccines have been approved. The mRNA vaccine from Pfizer/BioNTech (Comirnaty, BNT162b2; BNT) and the vector vaccine from AstraZeneca (Vaxzevria, ChAdOx1; AZ) have been widely used. mRNA vaccines induce high antibody and T cell responses, also to SARS-CoV-2 variants, but are costlier and less stable than the slightly less effective vector vaccines. Read More

View Article and Full-Text PDF

Effects of cryo-preservation on skeletal muscle tissues mechanical behavior under tensile and peeling tests until rupture.

J Mech Behav Biomed Mater 2022 May 17;132:105273. Epub 2022 May 17.

Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, Etablissement Francais du Sang, INSERM, U 1059 Sainbiose, Centre CIS, F, 42023, Saint-Etienne, France.

The most common method to study the mechanical behavior of soft tissue is to test animal specimens, which should be prepared as soon as possible after the death to avoid biological deterioration effects such as rigor mortis. Freezing and cryo-preservation could allow extending the time between procurement and implantation. From a mechanical perspective, tissue preservation could influence mechanical testing results. Read More

View Article and Full-Text PDF