237 results match your criteria Animal Cells and Systems[Journal]


Analyzing and validating the prognostic value and immune microenvironment of clear cell renal cell carcinoma.

Anim Cells Syst (Seoul) 2022 29;26(2):52-61. Epub 2022 Mar 29.

Department of urology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, People's Republic of China.

Tumor immune microenvironment (TIME) plays an important role in tumor diagnosis, prevention, treatment and prognosis. However, the correlation and potential mechanism between clear cell renal cell carcinoma ccRCC) and its TIME are not clear. Therefore, we aimed to identify potential prognostic biomarkers related to TIME of ccRCC. Read More

View Article and Full-Text PDF

Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis.

Anim Cells Syst (Seoul) 2022 15;26(2):45-51. Epub 2022 Mar 15.

Department of Anatomy, College of Medicine, Korea University, Seoul, Korea.

Tissue clearing for 3-dimensional (3D) imaging is increasingly utilized in many biomedical applications, including the pathological examination of human biopsy specimens. Although many protocols offer rapid and efficient tissue clearing (>1 d), immunofluorescence labeling of thick specimens is a highly time-consuming process. The use of low molecular weight chemical dyes has potential benefits in terms of speed and quality of 3D labeling. Read More

View Article and Full-Text PDF

Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A.

Anim Cells Syst (Seoul) 2022 10;26(2):70-83. Epub 2022 Apr 10.

Department of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of Korea.

Owing to their self-renewal and differentiation abilities, spermatogonial stem cells (SSCs) are essential for maintaining male fertility and species preservation through spermatogenesis. With an increase in exposure to plasticizers, the risk of endocrine-disrupting chemicals exerting mimetic effects on estrogen receptors, such as bisphenol A (BPA), has also increased. This has led to concerns regarding the preservation of male fertility. Read More

View Article and Full-Text PDF

Characterization of differential gene expression of broiler chicken to thermal stress in discrete developmental stages.

Anim Cells Syst (Seoul) 2022 5;26(2):62-69. Epub 2022 Apr 5.

Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Korea.

Prolonged exposure to high temperatures is linked to a range of physiological responses in broiler chickens including reduced disease resistance, low growth rate, and high mortality rate. In this study, we investigated the effect of heat stress on gene expression levels in 4-week-old and 6-week-old chickens each exposed to environments conditioned at thermoneutral (21 °C) and high (32 °C) temperatures. The analysis of differentially expressed genes (DEGs) using microarray revealed that genes underlying reactive oxygen species (ROS) production, cell nutrient intake, glucose metabolism, and circadian rhythm were differentially regulated in association with heat stress. Read More

View Article and Full-Text PDF

Deletion of IP6K1 in mice accelerates tumor growth by dysregulating the tumor-immune microenvironment.

Anim Cells Syst (Seoul) 2022 31;26(1):19-27. Epub 2022 Jan 31.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

A family of inositol hexakisphosphate kinases (IP6Ks) catalyzes the production of inositol pyrophosphate IP (5-diphosphoinositolpentakisphosphate) which is known to modulate various biological events such as cell growth. While targeting IP6K1 in various cancer cells has been well reported to control cancer cell motility and invasiveness, the role of host IP6K1 in tumor progression remains unknown. By using a syngeneic MC38 murine mouse colon carcinoma model, here we examined how host IP6K1 in the tumor microenvironment influences tumor growth. Read More

View Article and Full-Text PDF
January 2022

Synapto-protective effect of lithium on HIV-1 Tat-induced synapse loss in rat hippocampal cultures.

Anim Cells Syst (Seoul) 2022 27;26(1):1-9. Epub 2021 Dec 27.

Department of Physiology, College of Medicine, Dankook University, Cheonan, Republic of Korea.

Human immunodeficiency virus type I (HIV-1) infection of the CNS produces synapse loss which correlates with cognitive decline in patients with HIV-associated neurocognitive disorders (HAND). Lithium is mood stabilizer of unknown mechanism used to treat bipolar disorder and is known to exhibit neuroprotective properties. Here, we studied the effects of lithium on HIV-1 Tat-induced synapses between rat hippocampal neurons. Read More

View Article and Full-Text PDF
December 2021

Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α.

Anim Cells Syst (Seoul) 2022 16;26(1):10-18. Epub 2022 Jan 16.

School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.

Although simvastatin has been shown to inhibit vascular permeability, which might be amplified via gap junction intercellular communication (GJIC), the underlying mechanism of action remains unclear. In the present study, we investigated the effects and mechanisms of simvastatin on endothelial cells GJIC. Specifically, human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α (10 ng/mL) alone or in combination with simvastatin (5 µM), and their effects on vascular endothelial cell GJIC tested via the scrape loading/dye transfer (SL/DT) assay. Read More

View Article and Full-Text PDF
January 2022

Kazinol C from stimulates autophagy via endoplasmic reticulum stress-mediated signaling.

Anim Cells Syst (Seoul) 2022 10;26(1):28-36. Epub 2022 Jan 10.

Department of Biological Sciences and Cellular Heterogeneity Research Center, Sookmyung Women's University, Seoul, Republic of Korea.

Autophagy modulators are considered putative therapeutic targets because of the role of autophagy in cancer progression. Kazinol C, a 1,3-diphenylpropane from the plant , has been shown to induce apoptosis in colon cancer cells through the activation of AMPK at high concentrations. In the present study, we found that Kazinol C induced autophagy through endoplasmic reticulum stress-mediated unfolded protein response signaling in several normal and cancer cell lines at low concentrations of Kazinol C that did not induce apoptosis. Read More

View Article and Full-Text PDF
January 2022

Effects of black garlic on the pacemaker potentials of interstitial cells of Cajal in murine small intestine and on gastrointestinal motility .

Anim Cells Syst (Seoul) 2022 10;26(1):37-44. Epub 2022 Mar 10.

Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Republic of Korea.

Black garlic (BG) is a newly explored food stuff obtained via fermentation of raw, healthy garlic, especially in Asian countries. Interstitial cells of Cajal (ICC) are the pacemaker cells of gastrointestinal (GI) motility. The purpose of this study was to investigate the effects of BG extract on the pacemaker potentials of the ICC in the small intestines of mice and the possibility of controlling GI motility. Read More

View Article and Full-Text PDF

Phylogeographic study of the species complex, with emphasis on Northeast Asia.

Anim Cells Syst (Seoul) 2021 20;25(6):434-444. Epub 2021 Dec 20.

Conservation Genome Resource Bank for Korean Wildlife, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, South Korea.

We conduct a phylogeographic and population genetic study of the Asiatic toad () to understand its evolutionary history, and the influence of geology and climate. A total of 292 individuals from 94 locations were genotyped for two mitochondrial loci (, ) and five nuclear introns (, , , , and ), and we performed a suite of phylogenetic, population genetic, and divergence dating analyses. The phylogenetic trees constructed using mitochondrial loci inferred being divided into two major groups: China mainland and Northeast Asia (Northeast China, Russia, and Korean Peninsula). Read More

View Article and Full-Text PDF
December 2021

Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination.

Anim Cells Syst (Seoul) 2021 25;25(6):424-433. Epub 2021 Nov 25.

Department of Biotechnology, Sangmyung University, Seoul 03016, Korea.

The harmful jellyfish Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean and characterized the molecular features of the rDNA. Read More

View Article and Full-Text PDF
November 2021

Detecting the differential genomic variants using cross-population phenotype-associated variant (XP-PAV) of the Landrace and Yorkshire pigs in Korea.

Anim Cells Syst (Seoul) 2021 29;25(6):416-423. Epub 2021 Nov 29.

Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju, Republic of Korea.

Although there have been many genome-wide association studies (GWAS) and selective sweep analyses to understand pig genomic regions related to growth performance, these methods considered only the gene effect and selection signal, respectively. In this study, we suggest the cross-population phenotype associated variant (XP-PAV) analysis as a novel method to determine the genomic variants with different effects between the two populations. XP-PAV analysis could reveal the differential genetic variants between the two populations by considering the gene effect and selection signal simultaneously. Read More

View Article and Full-Text PDF
November 2021

Grub polypeptide extracts protect against oxidative stress through the NRF2-ARE signaling pathway.

Anim Cells Syst (Seoul) 2021 27;25(6):405-415. Epub 2021 Dec 27.

Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child Health Care Hospital, Capital Medical University, Beijing, People's Republic of China.

Grub polypeptide extracts (GPEs) have antioxidant effects; however, their underlying molecular mechanisms are unknown. This study explored the antioxidant molecular mechanism of GPE via the nuclear factor-erythroid 2-related factor 2 (NRF2)-antioxidant response element (ARE) signaling pathway in C2C12 muscle satellite cells exposed to oxidative stress. The effects of GPE/or H2O2 on C2C12 were investigated by the MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) viability assay and immunofluorescence and small interfering RNA (siRNA) analyses. Read More

View Article and Full-Text PDF
December 2021

extract improves metabolic derangements in high fructose diet-fed rats.

Anim Cells Syst (Seoul) 2021 22;25(6):396-404. Epub 2021 Nov 22.

Department of Biology, Jeju National University, Jeju, Republic of Korea.

has been reported to possess beneficial properties for human health, but its effects on metabolic syndrome have not been reported. In this study, we investigated the effect of leaf extract (CT) on the metabolic derangements induced by a high-fructose (HF) diet. Sprague-Dawley rats were fed with a 46% carbohydrate diet (HC group), 60% high-fructose diet (HF group), or HF diet supplemented with CT (500 mg/kg of body weight/day, CT group) via drinking water for 16 weeks. Read More

View Article and Full-Text PDF
November 2021

Mirodenafil ameliorates skin fibrosis in bleomycin-induced mouse model of systemic sclerosis.

Anim Cells Syst (Seoul) 2021 3;25(6):387-395. Epub 2021 Nov 3.

Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.

Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by fibrosis of the skin and internal organs. Despite the recent advances in the pathogenesis and treatment of SSc, effective therapies for fibrosis caused by SSc have not yet been established. In this study, we investigated the potential role of mirodenafil, a potent phosphodiesterase 5 (PDE5) inhibitor, in the treatment of fibrosis in SSc. Read More

View Article and Full-Text PDF
November 2021

Short-term maintenance on a high-sucrose diet alleviates aging-induced sleep fragmentation in .

Anim Cells Syst (Seoul) 2021 3;25(6):377-386. Epub 2021 Nov 3.

Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon, Republic of Korea.

Sleep is a fundamental behavior in an animal's life influenced by many internal and external factors, such as aging and diet. Critically, poor sleep quality places people at risk of serious medical conditions. Because aging impairs quality of sleep, measures to improve sleep quality for elderly people are needed. Read More

View Article and Full-Text PDF
November 2021

Oxytocin-induced anxiogenic behavior in juvenile male rats.

Anim Cells Syst (Seoul) 2021 31;25(6):369-376. Epub 2021 Oct 31.

Department of Science Education, Dankook University, Yongin-si, Republic of Korea.

Oxytocin (OT) is considered beneficial to mental health owing to its anxiolytic, prosocial, and anti-stress effects; however, the adverse effects of OT have been controversial, such as its potentially anxiogenic actions. Although OT influences drug abuse and reciprocally affects vulnerability to drug use, the relationship between OT's anxiogenic working and nicotine preference intake has not been clearly defined. To clarify this issue, the effect of acute peripheral administration of OT on anxiety and nicotine preference was investigated in juvenile male rats. Read More

View Article and Full-Text PDF
October 2021

Syncytin, envelope protein of human endogenous retrovirus (HERV): no longer 'fossil' in human genome.

Anim Cells Syst (Seoul) 2021 12;25(6):358-368. Epub 2022 Jan 12.

College of Natural Sciences, Hanyang University, Seoul, Republic of Korea.

Human endogenous retroviruses (HERVs) are 'fossil viruses' that resulted from stable integrations of exogenous retroviruses throughout evolution. HERVs are defective and do not produce infectious viral particles. However, some HERVs retain a limited coding capacity and produce retroviral transcripts and proteins, which function in human developmental process and various pathologies, including many cancers and neurological diseases. Read More

View Article and Full-Text PDF
January 2022

Inositol Requiring Enzyme (IRE), a multiplayer in sensing endoplasmic reticulum stress.

Anim Cells Syst (Seoul) 2021 10;25(6):347-357. Epub 2022 Jan 10.

Department of Biochemistry, University of Alberta, Edmonton, Canada.

The endoplasmic reticulum (ER) can sense a wide variety of external and internal perturbations and responds by mounting stress coping responses, such as the unfolded protein response (UPR). The UPR is composed of three stress sensors, namely IRE1α, PERK, and ATF6 that are activated to re-establish ER homeostasis. IRE1α represents the most ancient branch of the UPR affecting many cellular processes in plant and animal cells. Read More

View Article and Full-Text PDF
January 2022

Vasopressin and oxytocin expression in hypothalamic supraoptic nucleus and plasma electrolytes changes in water-deprived male .

Anim Cells Syst (Seoul) 2021 10;25(5):337-346. Epub 2021 Oct 10.

Faculty of Biological Sciences, Laboratory of Biology and Physiology of Organisms, Neurobiology, University of Sciences and Technology Houari Boumediene (USTHB), Algiers, Algeria.

In mammals, plasmatic osmolality needs to be stable, and it is highly related to the hydric state of the animals which depends on the activity of the hypothalamic neurohypophysial system and more particularly by vasopressin secretion. Meriones, a desert rodent, can survive even without drinking for more than one month. The mechanism(s) by which they survive under these conditions remains poorly understood. Read More

View Article and Full-Text PDF
October 2021

Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3).

Anim Cells Syst (Seoul) 2021 6;25(5):323-336. Epub 2021 Oct 6.

Laboratory of Biochemistry, Division of Life Sciences, Korea University, Seoul, Republic of Korea.

Ribosomal protein S3 (rpS3), a member of 40S small ribosomal subunit, is a multifunctional protein with various extra-ribosomal functions including DNA repair endonuclease activity and is secreted from cancer cells. Therefore, antibodies with high specificity against rpS3 protein could be useful cancer biomarkers. In this study, polyclonal antibody (pAb) and monoclonal antibodies (mAbs) were raised against rpS3 protein and epitope mapping was performed for each antibody; the amino acid residues of rpS3 were scanned from amino acid 185 to 243 through peptide scanning to reveal the epitopes of each mAb. Read More

View Article and Full-Text PDF
October 2021

UCH-L1 and UCH-L3 regulate the cancer stem cell-like properties through PI3 K/Akt signaling pathway in prostate cancer cells.

Anim Cells Syst (Seoul) 2021 11;25(5):312-322. Epub 2021 Oct 11.

Department of Biological Sciences, Inha University, Incheon, Korea.

Castration-resistant prostate cancer (CRPC) is a highly aggressive and advanced prostate cancer that is currently incurable with conventional therapies. The recurrence and chemotherapy-resistant properties of CRPC are attributed to prostate cancer stem cells (CSCs). On the other hand, the factors regulating the prostate CSC-like properties have not been studied extensively. Read More

View Article and Full-Text PDF
October 2021

Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study.

Anim Cells Syst (Seoul) 2021 28;25(5):296-311. Epub 2021 Sep 28.

Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin, People's Republic of China.

The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FA-MSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. Read More

View Article and Full-Text PDF
September 2021

Se improves GPX4 expression and SOD activity to alleviate heat-stress-induced ferroptosis-like death in goat mammary epithelial cells.

Anim Cells Syst (Seoul) 2021 17;25(5):283-295. Epub 2021 Oct 17.

College of Chemistry & Pharmacy, Northwest Agricultural & Forestry University, Yangling, People's Republic of China.

Selenium (Se) is a vital element of life, which has an important impact on the growth, development, production performance and stress-tolerance of animals. However, it is not entirely clear that how exactly Se works during these processes. Herein, we investigate the role of Se in regulating the functions of goat mammary epithelial cells (GMECs) under heat-stress condition. Read More

View Article and Full-Text PDF
October 2021

Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice.

Anim Cells Syst (Seoul) 2021 8;25(5):272-282. Epub 2021 Sep 8.

Department of Biomedical Science, Daegu Catholic University, Gyeongsan-Si, Republic of Korea.

(Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0. Read More

View Article and Full-Text PDF
September 2021

Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model.

Anim Cells Syst (Seoul) 2021 25;25(5):264-271. Epub 2021 Oct 25.

Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University Ansan Hospital, Korea University College of Medicine, Seoul, Republic of Korea.

Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effects. Read More

View Article and Full-Text PDF
October 2021

expression in the pharyngeal pouch is dispensable for craniofacial development.

Anim Cells Syst (Seoul) 2021 27;25(5):255-263. Epub 2021 Aug 27.

Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, South Korea.

Epidermal growth factor-like domain multiple 6 (Egfl6) is a basement membrane protein and plays an important role in hair follicle morphogenesis, angiogenesis, notochord development in vertebrates. Although expression in the developing head was observed in zebrafish, its role for craniofacial development and the determination of the pharyngeal region expressing , have not been reported yet. Here, we report the expression patterns and function of in craniofacial development in zebrafish. Read More

View Article and Full-Text PDF

Opposite functions of RapA and RapC in cell adhesion and migration in .

Anim Cells Syst (Seoul) 2021 1;25(4):203-210. Epub 2021 Jul 1.

Department of Biology & BK21- Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju, Republic of Korea.

There are three Rap proteins in . RapA is a key regulator of cell adhesion and cytoskeletal rearrangement. Recently, RapC has been reported to be involved in cytokinesis, cell migration, and multicellular development. Read More

View Article and Full-Text PDF

Time-sequential change in immune-related gene expression after irradiation in glioblastoma: next-generation sequencing analysis.

Anim Cells Syst (Seoul) 2021 30;25(4):245-254. Epub 2021 Jul 30.

Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, Republic of Korea.

The time-sequential change in immune-related gene expression of the glioblastoma cell line after irradiation was evaluated to speculate the effect of combined immunotherapy with radiotherapy. The U373 MG glioblastoma cell line was irradiated with 6 Gy single dose. Next-generation sequencing (NGS) transcriptome data was generated before irradiation (control), and at 6, 24, and 48 h post-irradiation. Read More

View Article and Full-Text PDF

MKP-3 suppresses LPS-induced inflammatory responses in HUVECs via inhibition of p38 MAPK/NF-κB pathway.

Anim Cells Syst (Seoul) 2021 14;25(4):235-244. Epub 2021 Jul 14.

Department of Molecular Medicine, College of Medicine, Inha University, Incheon, Republic of Korea.

Endothelial cell dysfunction and inflammatory responses play critical roles in the development of atherosclerosis. Recent data on the processes underlying atherogenesis indicate the substantial role of endotoxins (lipopolysaccharides; LPS) of the intestinal microflora in the initiation and progression of atherosclerosis. Mitogen-activated protein (MAP) kinase phosphatase-3 (MKP-3) is a cytoplasmic dual-specificity protein phosphatase that specifically binds to and inactivates MAP kinases in mammalian cells, but its biological function in endothelial cell dysfunction and inflammatory responses remains largely unknown. Read More

View Article and Full-Text PDF