Search Our Scientific Publications & Authors

Anim Biotechnol 2020 Dec 2:1-10. Epub 2020 Dec 2.

Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.

The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed's adaptability to the area and disease resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regenerative medicine and species conservation, the aims of this study were to isolate and characterize ESCs of TCC. Read More

Anim Biotechnol 2021 May 9:1-3. Epub 2021 May 9.

ICAR-National Bureau of Animal Genetic Resources, Karnal, India.

The grey type donkeys of region of Uttar Pradesh in India were genetically characterized using ten heterologous microsatellite loci. At these loci the PCR product size ranged from 75-95 bp at locus HTG6 to 251-277 bp at locus COR18. The observed number of alleles varied from 4 (HTG15) to 10 (HTG7 and AHT4) with a mean of 7. Read More

Anim Biotechnol 2021 May 6:1-8. Epub 2021 May 6.

Department of DETO - Section of Veterinary Science and Animal Production, University of Bari "Aldo Moro", Valenzano, Italy.

This study was carried out to determine the effect of dietary flaxseed meal (FSM) supplemented with dried tomato pomace (DTP) and dried grape pomace (DGP) on performance, egg quality, biochemical parameters traits and antioxidant status of laying hens. Birds (1825 ± 87 g of body weight) were divided into 12 dietary groups with six replicates per group (eight birds per replicate), under a completely randomized design with factorial arrangement 2 × 3 × 2 consisted of two levels of DTP (0 and 15%), three FSM levels (0, 4 and 8%) and two levels of DGP (0 and 5%). As a result of this study, there were no significant differences in egg production and weight as well in feed conversion ratio (FCR) among treatments ( > 0. Read More

Anim Biotechnol 2021 May 4:1-6. Epub 2021 May 4.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Aryl Hydrocarbon Receptor (AHR) is a member of the PER-ARNT-SIM (PAS) family, which could mediate various biological processes, for instance, the balance of the immune system, cell proliferation, differentiation, vascular tissue remodeling and reproduction ability regulation. A previous research showed that the gene exerted important functions on the pig reproduction, implying that it could serve as a candidate gene related to animal reproductive traits. Here, the aim of this work was to identify potential insertion/deletion (indel) mutations of the gene in three sheep breeds and analyze the associations between these mutations and reproductive traits. Read More

Anim Biotechnol 2021 May 4:1-9. Epub 2021 May 4.

Department of Food Engineering and Technology, ICT, Mumbai, India.

Meat is traditionally obtained by sacrificing the animals. It is considered as one of the richest sources of proteins. There is an increasing demand for meat worldwide. Read More

Anim Biotechnol 2021 May 3:1-9. Epub 2021 May 3.

Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Sultanate of Oman.

This study was conducted to examine the effects of neem () seed oil (NSO) on feed intake, ruminal fermentability, feed digestibility, nitrogen balance, and blood parameters in Omani sheep. Nine Omani male sheep (BW; 54.44 ± 8 kg/sheep) were used in a replicated 3 × 3 Latin Square design with three dietary treatments and three periods, with each period lasting 21 days. Read More

Anim Biotechnol 2021 May 3:1-9. Epub 2021 May 3.

College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.

Utilization of female livestock can be optimized by application of Fixed-Time Artificial Insemination (FTAI), which plays an important role in large-scale livestock production. However, molecular mechanism of FTAI affecting reproductive performance remains unclear. To investigate the effects of FTAI on corpus luteum in 16 and 25 days of pregnancy gilts, 12 pregnancy gilts were selected from Altrenogest + PMSG + GnRH (APG) group and control group. Read More

Anim Biotechnol 2021 May 3:1-6. Epub 2021 May 3.

Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; State Key Laboratory of Grassland Agro-ecosystems; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.

The present study was undertaken to elucidate the direct ALA effects on mice TM4 Sertoli cells proliferation . Our results showed that TM4 cells viability was significantly stimulated by ALA ( 0.05). Read More

Anim Biotechnol 2021 May 3:1-6. Epub 2021 May 3.

Key Laboratory of Feed Biotechnology of Agricultural Ministry, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

This study was examined to investigate the effect of Azomite (AZO) on the growth performance, immune function, and bone mineralization of broiler chickens. A total of 240-d old male chicks were randomly assigned into four treatments with six replicates ( = 10), which included control (basal diet), basal diet +0.25% AZO, basal diet + 0. Read More

Anim Biotechnol 2021 Apr 30:1-7. Epub 2021 Apr 30.

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, P. R. China.

Thirty castrated Duroc × Landrace × Yorkshire (DLY) pigs were randomly divided into three groups and slaughtered at 180, 210, and 240 days of age, respectively. Here, we found that the live weight, carcass weight, carcass length, dressing percentage, eye muscle area, backfat deposit, muscle yellowness b* value, drip loss, and cooking loss increased significantly, and the muscle pH 45 min value decreased dramatically as the slaughter age of DLY pigs extended. Moreover, increasing the slaughter age of DLY pigs could obtain higher n-3 polyunsaturated fatty acid (PUFA) percentage, crude protein, essential amino acids (EAA) contents and EAA/NEAA level, and lower n-6/n-3 PUFA level and antioxidant capacity. Read More

Anim Biotechnol 2021 Apr 30:1-9. Epub 2021 Apr 30.

Department of Veterinary Pathology, College of Veterinary Science (GADVASU), Rampura Phul, Bathinda, Punjab, India.

Casein constitutes approximately 80% of the total protein in bovine milk and is regarded as a high-quality dietary protein embracing all the nine essential amino acids. However, the contested physiological effect of a bioactive peptide released upon digestion of a β-casein milk protein variant originating from a cow of a particular genetic makeup has evoked wide interest in research and industry. Present investigations were carried out to genotype the polymorphism in milk β-casein gene, delineate the seasonal, periodic, and parity variations in production and reproduction traits, and examine the genetic association between β-casein genotypes and production, and reproduction traits in Vrindavani crossbred cows. Read More

Anim Biotechnol 2021 Apr 30:1-6. Epub 2021 Apr 30.

ICAR-Directorate of Poultry Research, Hyderabad, Telangana, India.

Avian oncogenic or tumor diseases are common in poultry industry causing significant economic loss. Marek's disease (MD), avian leukosis (AL) and Reticuloendotheliosis (RE) are the three major viral oncogenic infections that are difficult to differentiate with gross lesions. Multiplex PCR for simultaneous detection and differentiation of these three viruses was developed and validated. Read More

Anim Biotechnol 2021 Apr 29:1-11. Epub 2021 Apr 29.

Key Laboratory of Ministry of Education, Ministry of Education, Southwest Minzu University, Chengdu, China.

As a member of the MicroRNA s (miRNAs) family, miR-421 has been widely studied in regulating the proliferation and apoptosis of cancer cells a. However, there are still no reports on miR-421 in regulating adipocyte differentiation and its related mechanisms. Accordingly, this study aimed to investigate the potential involvement of miR-421 in goat intramuscular preadipocytes (P_IMA). Read More

Anim Biotechnol 2021 Apr 20:1-6. Epub 2021 Apr 20.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Cell division cycle 25 A () accounts for an essential function on early folliculogenesis of female mammals, especially regulating the function of intra-ovarian, thus this gene is pinpointed as a candidate gene that influences the kidding number of goat. On this ground, the purpose of this study was to investigate whether the reported 20-nt nucleotide variants locus (rs639467625) of the gene influences kidding number in Shaanbei white cashmere goat (SBWC). The -test showed that there were more ID genotypes in mothers of multiple lambs than in mothers of single lambs. Read More

Anim Biotechnol 2021 Apr 19:1-20. Epub 2021 Apr 19.

Department of Poultry Production, Faculty of Agriculture, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.

Growth and meat production are the most relevant targets for animal breeders, there are strong relationships between animal growth regulation, body composition and meat quality. Therefore, it is essential to identify the genetic factors that are controlling growth, carcass, and meat quality traits and to explore the correlations between identified genes of those traits. Identification of candidate genes may shift rabbit breeding from classical to modern approaches, which offer great potential to accelerate genetic improvement plans, especially in developing countries. Read More

Anim Biotechnol 2021 Apr 18:1-12. Epub 2021 Apr 18.

Maize Research Institute, Sichuan Agricultural University, Chengdu, China.

Intestinal epithelial restitution is partly dependent on cell migration, which reseals superficial wounding after injury. Here, we tested the hypothesis that stromal interaction molecule 1(STIM1) regulates porcine intestinal epithelial cell migration by activating transient receptor potential canonical 1 (TRPC1) signaling. Results showed that the knockdown of STIM1 repressed cell migration after wounding, reduced the protein concentration of STIM1 and TRPC1, and decreased the inositol trisphosphate (IP3) content in IPEC-J2 cells ( < 0. Read More

Anim Biotechnol 2021 Apr 18:1-8. Epub 2021 Apr 18.

Division of Pathology, ICAR - Indian Veterinary Research Institute, Izatnagar, India.

The present study was designed to evaluate the effect of factors like hormones, antisperm antibody (ASA), and oxidative stress and its relation with semen quality in crossbred bulls. Ejaculates from two bulls were categorized into good ( = 12) and poor ( = 12) based on initial progressive motility, that is, ≥70% and ≤50%, respectively. The level of hormones like Testosterone ( < 0. Read More

Anim Biotechnol 2021 Apr 13:1-11. Epub 2021 Apr 13.

College of Life Science, Northeast Agricultural University, Heilongjiang, Harbin, China.

Tudor staphylococcal nuclease (Tudor-SN) participates in milk synthesis and cell proliferation in response to prolactin (PRL) and plays a regulatory role on mTOR phosphorylation. However, the complicated molecular mechanism of Tudor-SN regulating milk protein synthesis and cell proliferation still remains to be illustrated. In present study, we observed that the proteins level of phosphorylated Tudor-SN and phosphorylated STAT5 were simultaneously enhanced upon PRL treatment in bovine mammary epithelial cells (BMECs). Read More

Anim Biotechnol 2021 Apr 13:1-7. Epub 2021 Apr 13.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.

DNA methylation could take part in the gene expression and acts an important role in muscle development. In this study, DNA methylation and expression in adipose and muscle tissues were examined at the same time to evaluate the extent of epigenetic modifications and gene expression on the differentially methylated region (DMR) in . Chain reaction of bisulfite sequencing polymerase (BSP) was used to compared difference among DNA methylation patterns. Read More

Anim Biotechnol 2021 Apr 13:1-12. Epub 2021 Apr 13.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, P. R. China.

The purpose of this study was to investigate the role of urea-N recycling, rumen bacterial community, and rumen epithelial gene expression in nitrogen utilization of calves. Here, 12 Holstein calves were divided into two dietary treatments: a high-protein diet (HP, 173 g/kg), and a low-protein diet (LP, 125 g/kg). Urea-N kinetics was evaluated using urea-NN isotope labeling method. Read More

Anim Biotechnol 2021 Apr 12:1-10. Epub 2021 Apr 12.

Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India.

Q fever caused by is an important zoonosis and has great public health significance. A total of 905 clinical samples from 387 cattle [serum ( = 387); vaginal swabs ( = 387); milk ( = 131)] and 59 serum samples from humans were collected from gaushala (cattle shelter) and screened for anti- IgG antibodies in the sera using an indirect-ELISA kit. Further, the samples were tested for DNA employing TaqMan real-time and conventional PCR assays targeting the 1 gene. Read More

Anim Biotechnol 2021 Apr 10:1-14. Epub 2021 Apr 10.

Faculty of Veterinary Medicine, Department of Animal Breeding, Fırat University, Elazig, Turkey.

The characterization of miRNAs from sheep milk and their effect on milk yield and composition in sheep are remain unclear. Therefore, the aim of this study was to determine the expression pattern of several important miRNAs, which are associated with lactation in the sheep milk between high and low lactating-yield ewe groups. In addition to experimentally obtained miRNA expression results, the miRNA target genes were determined by bioinformatics analysis to identify biological pathways involved. Read More

Anim Biotechnol 2021 Apr 10:1-10. Epub 2021 Apr 10.

ICAR-Central Institute for Research on Buffaloes, Hisar, India.

Avian mycoplasmosis mainly caused by and is an economically important disease of poultry industry. It causes huge economic losses in terms of decrease in weight gain, feed conversion efficiency, egg production, hatchability; increase in embryo mortality, carcass condemnation, prophylaxis and treatment cost in broiler, layer and breeder flocks. The disease is caused by four major pathogenic mycoplasmas . Read More

Anim Biotechnol 2021 Apr 7:1-13. Epub 2021 Apr 7.

College of Animal Sciences, Jilin University, Changchun, China.

Carnitine palmitoyltransferase 1B (CPT1B) is a candidate gene that regulates livestock animal lipid metabolism and encodes the rate-limiting enzyme in fatty acid β-oxidation. To explore the effect of this gene on lipid metabolism in cattle, this study examined CPT1B gene polymorphism in Chinese Simmental cattle and the effect of CPT1B on lipid metabolism. The results showed that the triglyceride content increased significantly with increasing CPT1B gene expression in bovine fetal fibroblasts (BFFs) ( < 0. Read More

Anim Biotechnol 2021 Apr 7:1-7. Epub 2021 Apr 7.

Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.

is mainly expressed in embryonic development, and it is reported to take an effect on the growth performance of mice, cattle, pigs, and sheep. To explore how conservative the is in different sheep breeds, the effects of the two indel variants on the growth traits of the Chinese Luxi blackhead (LXBH) sheep were firstly detected. The P2-del 30 bp and P4-del 45 bp indel loci of the sheep gene were significantly related to 15 growth traits ( < 0. Read More

Anim Biotechnol 2021 Mar 29:1-12. Epub 2021 Mar 29.

Department of Animal Genetics and Breeding, Dr. G. C. Negi College of Veterinary and Animal Sciences, Palampur, India.

Heat shock proteins (HSPs) act as a chaperone activity ensuring the folding, unfolding, and refolding of denatured proteins, which help in a protective role during thermal stress in dairy cattle. This study aimed to detect genetic variations of the HSPB6 gene and to determine their association with heat tolerance traits in Karan Fries cattle. Five single nucleotide polymorphisms (SNPs) (SNP 1-5) were reported in the Karan Fries cattle, which included three transitions viz. Read More

Anim Biotechnol 2021 Mar 28:1-13. Epub 2021 Mar 28.

Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, China.

In this study, the genes related to the Downy growth of Liaoning cashmere goats were screened for their expression with simultaneous melatonin administration, so as to investigate the effects of target genes on the proliferation of skin fibroblasts in this animal species. Genes related to the villus growth of skin fibroblasts were screened by in vitro transcriptome sequencing and verified by qPCR. In addition, gene overexpression and interference were used to study the effects of target genes on the proliferation of skin fibroblasts. Read More

Anim Biotechnol 2021 Mar 24:1-12. Epub 2021 Mar 24.

Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural and Natural Resources University, Sari, Iran.

Mastitis disease causes significant economic losses in dairy farms by reducing milk production, increasing production costs, and reducing milk quality. continues to be a major cause of mastitis in dairy cattle. To date, there has been no approved multi-epitope vaccine against this pathogen in the market. Read More

Anim Biotechnol 2021 Mar 22:1-12. Epub 2021 Mar 22.

Agricultural and Natural Resources Research and Education Center of Khorasan Razavi, Agricultural Research, Education and Extension Organization (AREEO), University of Torbat-e Jam, Mashhad, Iran.

The nutritional value of some tree leaves (grape, common fig, barberry, sweet cherry, apricot, peach, and oleaster) was investigated by and common laboratory methods. Also, thirty Baluchi male lambs were randomly divided into five groups, including one control group (alfalfa) and four experimental groups, which received diets containing different leaves (barberry, apricot, peach, and grape). The crude protein content ranged from 128 for the sweet cherry to 164 g/kg DM for grape. Read More

Anim Biotechnol 2021 Mar 22:1-6. Epub 2021 Mar 22.

College of Veterinary Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.

The plants originated from an agroforestry system have gained immense importance in livestock health and production. The trial was planned to examine the effects of leaf extract (MLE) supplementation on the performance, blood metabolites, immunity and lipid peroxidation in broiler chicks. A total of 350 day old broiler chicks were divided into negative control (MLE), positive control (antibiotic; AB-enro), supplementation at the concentration of 60 (MLE), 90 (MLE) and 120 mL/L (MLE) of drinking water. Read More