1,150 results match your criteria Animal Biotechnology[Journal]


Development and evaluation of polyclonal antibodies based antigen capture ELISA for detection of porcine rotavirus.

Anim Biotechnol 2022 May 20:1-8. Epub 2022 May 20.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Rotaviruses are rising as zoonotic viruses worldwide, causing the lethal dehydrating diarrhea in children, piglets, and other livestock of economic importance. A simple, swift, cost-effective, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) was developed for detection of porcine rotavirus-A (PoRVA) by employing rabbit (capture antibody) and murine polyclonal antibodies (detector antibody) produced against VP6 of PoRVA (RVA/Pig-tc/CHN/TM-a/2009/G9P23). Reactivity of the both polyclonal antibodies was confirmed by using an indirect ELISA, western-blot analysis and indirect fluorescence assay against rVP6 protein and PoRVA. Read More

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Investigation on mRNA expression and genetic variation within goat gene and its association with litter size.

Anim Biotechnol 2022 May 18:1-9. Epub 2022 May 18.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

The (), a member of the TGF-beta superfamily, executes a significant part in the oogenesis and ovulation process. A genome-wide selective sweep analysis also found was different in the fertility groups of Laoshan dairy goats; whether this gene was linked to litter size was unknown. Therefore, was chosen to study its effects on Shaanbei white cashmere goat reproduction and mRNA expression profile. Read More

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Effect on physiological parameters and semen quality upon oral administration of fresh onion juice to V-line rabbit buck during severe heat stress.

Anim Biotechnol 2022 May 11:1-9. Epub 2022 May 11.

Animal Production Department, Faculty of Agriculture, Omer Al-Mukhtar University, Bieda, Libya.

Severe heat stress is recognized as a factor that severely influences the semen quality and antioxidant status of rabbits. In this context, fresh onion juice (FOJ) may be a safe and useful option to improve reproductive performance. This research was designed to evaluate the anti-stress effects of FOJ on physiological and semen parameters in heat-stressed bucks. Read More

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Expression analysis of TLR signaling pathway genes under lipopolysaccharide-induced and F17-infected sheep intestinal epithelial cells.

Anim Biotechnol 2022 May 11:1-7. Epub 2022 May 11.

College of Animal Science and Technology, Yangzhou University, Yangzhou, PR China.

() F17 is one of the main pathogens causing diarrhea in young livestock. The specific F17 fimbriae and lipopolysaccharide (LPS) in the surface components of F17 induces immune activation interacting with the intestinal epithelial cells (IECs)-expressed innate immune toll-like receptors (TLRs) signaling pathway. In this study, the expression patterns of eight canonical genes from the TLR signaling pathway (, , , , , , and ) were analyzed in LPS-induced IECs, F17-infected IECs and ileum tissue of F17-infected lambs. Read More

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Evolutionary analysis of buffalo sterol regulatory element-binding factor (SREBF) family genes and their affection on milk traits.

Anim Biotechnol 2022 May 9:1-12. Epub 2022 May 9.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.

The sterol regulatory element-binding factor (SREBF) genes are a vital group of proteins binding to the sterol regulatory element 1 () regulating the synthesis of fatty acid. Two potential candidate genes ( and ) have been identified as affecting milk traits. This study aims to identify the SREBF family of genes and find candidate markers or SREBF genes influencing lactation production in buffalo. Read More

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4 overexpression promotes apoptosis in goat turbinate bone cells.

Anim Biotechnol 2022 May 6:1-9. Epub 2022 May 6.

Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Key Laboratory of Sichuan Province, Chengdu, China.

Hyaluronic acid-binding protein (4) plays important roles in regulating cell cycle and apoptosis. However, its functions in regulating cell apoptosis remain unclear. To reveal the effects of 4 on cell proliferation, cell cycle and apoptosis, the 4 sequence was cloned, and we investigated the gain and loss functions of 4 in goat turbinate bone cells. Read More

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Study on the role of heat shock protein 90 () gene in chicken preadipocytes proliferation and differentiation.

Anim Biotechnol 2022 May 6:1-10. Epub 2022 May 6.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China.

In this study, we examined the effects of Heat Shock Protein 90 () on adipocyte proliferation and differentiation in chickens. To achieve this, we constructed RNA interference (RNAi) vectors to target and transfected the vectors into primary adipocytes. After transfection, oil red O staining was performed to determine the status of triglyceride accumulation in the cells, whereas the CCK-8 cell kit and 5-Ethynyl-2'-Deoxyuridine (EdU) assays were used to determine cell proliferation. Read More

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Effect of dietary concentrate level on digestibility of nutrients in each region of the gastrointestinal tract and rumen fermentation in goats.

Anim Biotechnol 2022 May 6:1-9. Epub 2022 May 6.

Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China.

This study evaluated the effects of high concentrate diets (HCD) on the rumen fermentation and the digestibility of nutrients in different sites of the gastrointestinal tract (GIT) in goats. Four goats were used in a crossover design. The goats were fitted with a ruminal cannula and flexible T-cannulae proximal duodenum and terminal ileum. Read More

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Association analysis for SNPs of and genes with body size and other production traits in Liaoning Cashmere Goats.

Anim Biotechnol 2022 May 4:1-11. Epub 2022 May 4.

College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.

Liaoning cashmere goat (LCG) have tall bones, high cashmere production and outstanding meat production performance. In recent years, good breeding progress has not been made in terms of body size, meat yield, milk yield and other properties in terms of production. The study focused on the correlation between the SNPs of and genes with the body size performance, cashmere production and milk performance. Read More

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Activated macrophages of CD 163 gene edited pigs generated by direct cytoplasmic microinjection with CRISPR gRNA/Cas9 mRNA are resistant to PRRS virus assault.

Anim Biotechnol 2022 May 4:1-14. Epub 2022 May 4.

Division of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, Taiwan, Republic of China.

Porcine reproductive and respiratory syndrome virus (PRRSV) infects placental and lung macrophages, causing a global epidemic with economic loss. Attempts to develop an effective vaccine to control the disease have not been effective. Currently, developing PRRSV disease-resistant pigs via a gene editing (GE) strategy to mutate the PRRSV receptor or to delete the binding domain on the macrophage appears promising. Read More

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Effects of starter feeding and early weaning on developmental expressions of gene in liver and gene in rumen of lambs from birth to eighty-four days of age.

Anim Biotechnol 2022 May 4:1-8. Epub 2022 May 4.

College of Animal Science and Technology, Gansu Agriculture University, Lanzhou, China.

The early weaning and starter feeding have significant effects on lamb growth and digestive tract development. However, it is not clear whether the expression of genes could be affected by feeding starter and weaning. Therefore, a total of 102 Hu male neonatal lambs were randomly divided into 3 groups: fed with starter at 42d + weaned at 56d (group A), fed with starter at 7d + weaned at 28d (group B) and fed with starter at 7d + weaned at 56d (group C), to explore the effects of starter feeding and weaning age on developmental expressions of gene in liver and gene in rumen of Hu sheep. Read More

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Characterization of mitochondrial 12S rRNA gene of yellow-striped chevrotain () and white-spotted chevrotain () and development of a PCR-RFLP marker for the unambiguous identification of the species.

Anim Biotechnol 2022 May 3:1-8. Epub 2022 May 3.

Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka.

Tragulids hold a significant place in the evolutionary history of mammals since they represent the basal branch of ruminants. Only three genera of tragulids are being extant to date such as , and . In the genus , Sri Lankan chevrotains ( and ) are endemic to Sri Lanka while is present in India. Read More

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Growth performance, carcass characteristics, and blood biochemical indices of broilers affected by dietary organic acids blend's supplementation.

Anim Biotechnol 2022 May 2:1-6. Epub 2022 May 2.

Department of Food and Nutrition, College of Human Sciences and Design, King Abdualziz University, Jeddah, Saudi Arabia.

The objective of the present study was to explore the influence of organic acids cocktail (lactic-L plus formic-F acid, LF) in broiler diets on feed utilization, growth rate, blood indices and carcass traits. A total of 300 chicks were used and allotted to five treatment groups. The rations contained 0 additives (negative control-NC), basal diet + 0. Read More

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Insertion/deletions within the bovine gene and their association analysis with growth traits in three Chinese cattle breeds.

Anim Biotechnol 2022 May 1:1-8. Epub 2022 May 1.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

() gene, as a transcription factor, plays a vital role in animal growth and development, participating in many biological processes. The aim of this study was to ascertain Insertion/deletions (Indels) polymorphism within bovine gene in 679 Chinese adult cows and associate them with stature traits. Two Indels (named as Indel-3 and Indel-4, recorded as rs383545622 and rs525318770 in NCBI, respectively) were successfully genotyped by the Once PCR method, which was reliable, rapid and cost effective for simultaneous detection of two or more Indels. Read More

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Morphometric characteristics and microsatellite markers based diversity and differentiation recognizes the first prospective cattle breed from the Jharkhand state of India.

Anim Biotechnol 2022 Apr 26:1-13. Epub 2022 Apr 26.

ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India.

India is bestowed with immense cattle biodiversity with 50 registered breeds. However, the majority (59.3%) is yet not characterized. Read More

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Biological methylation: redefining the link between genotype and phenotype.

Anim Biotechnol 2022 Apr 25:1-13. Epub 2022 Apr 25.

Animal Genetics and Breeding Division, Veterinary College, Hassan, KVAFSU, Karnataka, India.

The central dogma of molecular biology is responsible for the crucial flow of genetic information from DNA to protein through the transcription and translation process. Although the sequence of DNA is constant in all organs, the difference in protein and variation in the phenotype is mainly due to the quality and quantity of tissue-specific gene expression and methylation pattern. The term methylation has been defined and redefined by various scientists in the last fifty years. Read More

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Expression profile and functional prediction of novel LncRNA 5.8S rRNA-OT1 in cattle.

Anim Biotechnol 2022 Apr 24:1-11. Epub 2022 Apr 24.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China.

Long non-coding RNAs (LncRNAs) are generally longer than 200 bp in length and play an important regulatory role in the growth and development of skeletal muscle. In the previous work, the non-coding RNAs with abundant expression in bovine tissues were screened out. After quantitative real-time PCR (qPCR), 33 lncRNAs with differential expression in various bovine tissues were identified. Read More

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Influence of tomato processing by-product extract as dietary supplementation on growth performance, carcass characteristics and antioxidant status of growing rabbits under high ambient temperature.

Anim Biotechnol 2022 Apr 22:1-10. Epub 2022 Apr 22.

Animal and Poultry Production Department, Faculty of Technology and Development, Zagazig University, Zagazig, Egypt.

This study evaluated the effects of dietary supplementation of tomato processing by-product extract (TPBE) on growth performance, carcass characteristics and antioxidant status of growing rabbits under high ambient temperature. A total of eighty weaned New Zealand White (NZW) male rabbits (6-weeks-old; initial body weight, 730.28 ± 36. Read More

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Bioinformatics and genetic variants analysis of gene promoter with their association at carcass quality and body measurement traits in Qinchuan beef cattle.

Anim Biotechnol 2022 Apr 21:1-10. Epub 2022 Apr 21.

College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.

The () gene regulates adipogenesis and myogensis. In this study, sequencing of prompter region identified three SNPs at loci g.78G > A, g. Read More

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Sequence variability of and gene in indigenous and crossbred cattle in Bangladesh.

Anim Biotechnol 2022 Apr 21:1-10. Epub 2022 Apr 21.

Animal Biotechnology Division, National Institute of Biotechnology, Dhaka, Bangladesh.

and genes are known to affect semen quality and fertility parameters, including sperm motility and maturation. However, studies are yet to examine the genes in indigenous and crossbred cattle in Bangladesh. Therefore, this study was conducted to determine the genetic variants of and in indigenous and crossbred cattle in Bangladesh. Read More

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Expression of miRNA-1-3p and its target gene in hair follicle cycle development of Liaoning Cashmere goat.

Anim Biotechnol 2022 Apr 20:1-6. Epub 2022 Apr 20.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China.

MicroRNA exerts an important regulatory role in almost all the biological process, including hair follicle development in Liaoning Cashmere goat. In order to improve the Cashmere performance of goat, the regulatory role of microRNA in hair follicle cycle has drawn hotspot attention. However, the molecular mechanisms of miRNA-1-3p involved in hair follicle development are poorly understood. Read More

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Molecular cloning and characterization of Cu-Zn superoxide dismutase () gene in brown trout and its expression in response to acute aquaculture stressors.

Anim Biotechnol 2022 Apr 19:1-11. Epub 2022 Apr 19.

Department of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, Erzurum, Turkey.

Aquaculture species are often exposed to acute stressors such as low water levels and handling during routine aquaculture procedures. This might result in oxidative stress by the increased reactive oxygen species (ROS)' production (, superoxide anion). The harmful effects of ROS are eliminated by a defense system, referred antioxidant defense system (ADS). Read More

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Transcriptomic analysis to elucidate the response of ligustica brain tissue to fluvalinate exposure.

Anim Biotechnol 2022 Apr 18:1-12. Epub 2022 Apr 18.

Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, China.

As a commonly used acaricide in apiculture, fluvalinate is used to kill Varroa mites, while it also damages the nervous system of honeybees. To date, the transcriptomic characteristics associated with fluvalinate-induced neuronal injury in the bee brain have not been reported. Here, we performed transcriptome sequencing on ligustica ( ligustica) brain tissues collected before and after fluvalinate treatment. Read More

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The impact of lactating Hu sheep's dietary protein levels on lactation performance, progeny growth and rumen development.

Anim Biotechnol 2022 Apr 13:1-12. Epub 2022 Apr 13.

Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.

This study aimed to investigate whether lactating Hu sheep's dietary protein levels could generate dynamic effects on the performance of their offspring. Twelve ewes with similar parity were fed iso-energy diets which contained different protein levels (P1: 9.82%, P2: 10. Read More

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Characterization of the fatty acid binding protein 3 (FABP3) promoter and its transcriptional regulation by cAMP response element binding protein 1 (CREB1) in goat mammary epithelial cells.

Anim Biotechnol 2022 Apr 13:1-8. Epub 2022 Apr 13.

College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, China.

Fatty acid binding protein 3 (FABP3) is involved in signal transduction pathways, and in the uptake and utilization of long-chain fatty acids. However, the transcriptional regulation of in goat is unclear. In this study, the 5' flanking region was amplified from goat () genomic DNA. Read More

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and development of interspecies Asian elephant embryos reconstructed with pig enucleated oocytes.

Anim Biotechnol 2022 Apr 11:1-10. Epub 2022 Apr 11.

Yunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Kunming, China.

Interspecies somatic cell nuclear transfer (iSCNT) has an immense potential to rescue endangered animals and extinct species like mammoths. In this study, we successfully established an Asian elephant's fibroblast cell lines from ear tissues, performed iSCNT with porcine oocytes and evaluated the and development of reconstructed embryos. A total of 7780 elephant-pig iSCNT embryos were successfully reconstructed and showed development with cleavage rate, 4-cell, 8-cell and blastocyst rate of 73. Read More

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Low frequency of protective variants at regulatory region of gene indicating the genetically high risk of BSE in Ethiopian and .

Anim Biotechnol 2022 Apr 9:1-6. Epub 2022 Apr 9.

Department of Biology Molecular Biology Section, Faculty of Science, Ege University, İzmir, Turkey.

Susceptibility to classical bovine spongiform encephalopathy (BSE) has been linked to 23 bp indel in promoter and 12 bp indel in the first intron of cattle prion protein gene. This study aimed to investigate 23/12 bp indel polymorphisms in the polymorphisms in cattle prion protein () gene to reveal the risk of BSE in Ethiopian cattle. Also, frequency of each polymorphism was compared to the other and breeds. Read More

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Genome-wide association studies and haplotype sharing analysis targeting the growth traits in Yandang partridge chickens.

Anim Biotechnol 2022 Apr 9:1-7. Epub 2022 Apr 9.

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

The body size of a chicken is an economically important trait as it directly influences the benefits of the poultry industry, but the relevant genetic mechanisms have not yet been elucidated. In this study, we measured eight growth traits for 94 Yandang partridge chickens, then undertook genome-wide association studies (GWAS) for those traits in using a linear mixed model based on 10× whole genomic sequencing data to better understand the knowledge of the genetic architecture of growth traits. Ninety-four individuals and 7647883 SNPs remained after quality control and removal of the sex chromosomes, and these data were used to carry out a GWAS analysis. Read More

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Effects of Compound Trace Minerals on the Growth Performance, Carcass Characteristics and Meat Quality of Fattening Pigs.

Anim Biotechnol 2022 Apr 10:1-6. Epub 2022 Apr 10.

Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Vietnam.

The objective of this study was to evaluate the effects of supplemental levels of compound minerals (CM), which was formulated from a mixture of 300 mg calcium, 150 mg magnesium, 25 mg zinc, 15 mg iron, 4 mg manganese, and 2 mg copper per gram, on the growth performance and feed conversion ratio of Duroc × (Landrace × Yorkshire) fattening pigs. A total of 53 female pigs and 45 male pigs at 35 days of age were individually notched and randomly allocated to three treatments, including0 (basal diet), 0.5 (basal diet with 0. Read More

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Nutritional significance and promising therapeutic/medicinal application of camel milk as a functional food in human and animals: a comprehensive review.

Anim Biotechnol 2022 Apr 7:1-18. Epub 2022 Apr 7.

Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Pakistan.

Camel milk (CM) is the key component of human diet specially for the population belongs to the arid and semi-arid regions of the world. CM possess unique composition as compare to the cow milk with abundant amount of medium chain fatty acids in fat low lactose and higher concentration of whey protein and vitamin C. Besides the nutritional significance of CM, it also contains higher concentration of bioactive compounds including bioactive peptides, lactic acid bacteria (LAB), lactoferrin (LF), lactoperoxidase, lysozyme casein and immunoglobulin. Read More

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