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Anim Biotechnol 2019 Apr 15:1-8. Epub 2019 Apr 15.

a ICAR-Indian Veterinary Research Institute , Bengaluru , Karnataka.

Interleukin 15 (IL-15) plays crucial role as stimulatory cytokine in generation and proliferation of CD8 T memory cells. The present study was initiated to analyze the role of yeast-expressed bovine IL-15 in inducing the CD8 T memory cells. The bIL-15 was cloned in pPICZαA expression vector. Read More

Anim Biotechnol 2019 Apr 15:1-10. Epub 2019 Apr 15.

a Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , People's Republic of China.

Prolactin receptor (PRLR) gene plays a crucial role in the milk production, reproduction and the growth of mammals. To fully characterize the structure of the mutation and to further study the function of the goat PRLR gene, two insertion/deletion (indel) loci (12 bp; 16-bp; 5-bp) were detected in 1038 Shaanbei white cashmere (SBWC) goats. Associated analysis revealed that the 16-bp indel mutation was significantly associated the body length, body height, chest depth (CD), body length index (BLI), heart girth index and cannon circumference index (CCI) (p < 0. Read More

Anim Biotechnol 2019 Apr 15:1-7. Epub 2019 Apr 15.

d ICAR , Krishi Bhavan , New Delhi , India.

Japanese encephalitis (JE) is a re-emerging mosquito-borne zoonotic flaviviral disease. Swine sero-convert 2-3 weeks before infection occurs in humans and thus serves as a suitable sentinel for JE surveillance and outbreak prediction in human population. The present study was conducted with the objective of developing a lateral flow assay (LFA) for detecting JEV antibodies in swine sera. Read More

Anim Biotechnol 2019 Apr 8:1-11. Epub 2019 Apr 8.

a College of Animal Science and Technology , Yangzhou University , Yangzhou , Jiangsu , China.

Chicken is popular among consumers in the market, but the mechanism for regulating its growth is still unclear. In this experiment, two groups of Bian chickens of different body weights at 16 weeks of age were studied. The leg muscles were taken for transcriptome sequencing after slaughter. Read More

Anim Biotechnol 2019 Apr 8:1-15. Epub 2019 Apr 8.

a Division of Fish Genetics and Biotechnology , ICAR-Central Institute of Fisheries Education , Mumbai , India.

Gonadotropin-inhibitory hormone (GnIH) plays an important role in reproduction by inhibiting the expression of gonadotropins in birds and mammals, but in fishes, it is ambiguous. In this study, we cloned 606 bp long cDNA of GnIH from Catla catla brain (cGnIH). The encoded preproGnIH peptide generated three putative peptides (cGnIH-I, -II, -III) of different size. Read More

Anim Biotechnol 2019 Apr 7:1-11. Epub 2019 Apr 7.

a Faculty of Life Sciences, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery , Liaoning Normal University , Dalian , China.

Liaoning Cashmere goat is a precious genetic resource of China. To explore the relationship between POMP and cashmere growth, we analyzed the expression of POMP. POMP encodes a hudrophilic protein which is most closely related to bos. Read More

Anim Biotechnol 2019 Apr 5:1-8. Epub 2019 Apr 5.

a Department of Genetics and Animal Breeding , Poznan University of Life Sciences , Poznan , Poland.

In humans, the dysfunction of the adenomatous polyposis coli (APC) gene causes hereditary familial adenomatous polyposis (FAP) and increased risk of colorectal cancer (CRC). The severity of polyposis varies between individuals, but genetic basis for this is in large part unknown. This variability also occurs in our porcine model of FAP, based on an APC mutation (orthologous to human APC). Read More

Anim Biotechnol 2019 Apr 5:1-3. Epub 2019 Apr 5.

a Key-Laboratory of Animal Science of State Ethnic Affairs Commission , Southwest Minzu University , Chengdu , Sichuan , China.

Hypoxia-inducible factors (HIFs) are oxygen-dependent transcriptional activators, but there is little information about their role in yak (Bos grunniens) reproduction. The present study, for the first time, investigated the adaptive mechanism of yak reproduction to high-altitude hypoxic stress by comparing the expression of HIF mRNAs between female yaks at high-altitude and cattle at low-altitude. Hypothalamus, anterior pituitary, oviduct, ovary and uterus tissue samples were collected from five adult female yaks and cattle. Read More

Anim Biotechnol 2019 Apr 5:1-6. Epub 2019 Apr 5.

a NutriGenomics Laboratory, Department of Poultry Science , University of Georgia , Athens , GA , USA.

The dietary requirement for cysteine is not determined in poultry since it is not an essential amino acid. The cysteine need is expected to be met through the transsulfuration pathway where homocysteine, a precursor of methionine, is converted to cysteine. Cysteine is a major component of plumage, and the degree to which cysteine is involved in plumage and other keratized proteins are unknown. Read More

Anim Biotechnol 2019 Mar 31:1-8. Epub 2019 Mar 31.

g Department of Animal Sciences , North Carolina Agricultural and Technical State University , Greensboro , NC , USA.

The objectives of the present study were to examine the impact of feeding both probiotics and sodium butyrate on calf performance and the economic implication of each treatment. A completely randomized design was used to investigate body weight (BW) gain, feed conversion efficiency and health conditions of Holstein dairy calves fed either pasteurized waste milk (PWM; n = 9) or a non-medicated milk replacer containing sodium butyrate and active probiotic Bacillus amyloliquefaciens (NMR; n = 9) from birth to 60 days of age. Numerically, calves fed PWM consumed more starter feed between days 16 and 45 than calves fed NMR but the difference became smaller by 60 days. Read More

Anim Biotechnol 2019 Mar 31:1-10. Epub 2019 Mar 31.

a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , People's Republic of China.

Serine protease inhibitor protein 3 (serpin peptidase inhibitor, clade A, member 3, SERPINA3) is a member of the serpin superfamily, probably related to the yield and quality of muscle. This study focuses on the relationship between SERPINA3 gene polymorphism and growth traits in beef cattle. The study first uses sequencing pooled DNA samples (Pool-Seq), PCR-RFLP and Tetra-primer ARMS-PCR techniques to determine the genetic polymorphisms of SERPINA3 in 765 beef cattle. Read More

Anim Biotechnol 2019 Mar 23:1-7. Epub 2019 Mar 23.

a Animal Biotechnology Centre , Nanaji Deshmukh Veterinary Science University , Jabalpur , Madhya Pradesh , India.

In the present study, the potential of different grades of cumulus-oocyte complexes (COCs) for in vitro maturation (IVM) and embryonic development was assessed. Further, the association of the expression pattern of anti-apoptotic Mcl-1 and pro-apoptotic Bax genes in embryonic development was analyzed. Abattoir derived oocytes were graded into grade A and B based on surrounding cumulus rings. Read More

Anim Biotechnol 2019 Mar 19:1-12. Epub 2019 Mar 19.

b Department of Biotechnology , Vikrama Simhapuri University , Nellore , India.

Melanocortin 1 receptor (MC1R) plays a vital role in melanogenesis and determines coat color of mammals. Polymorphic variants in MC1R, causing coat color variation, were described in few mammals; however, such studies were not done in cattle. The objective of the study was to explore the association of MC1R gene polymorphism within Tharparkar (Bos indicus) and Karan Fries (B. Read More

Anim Biotechnol 2019 Mar 19:1-8. Epub 2019 Mar 19.

a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.

Zinc finger protein 395 (ZNF395), belonging to the Krüppel C2H2-type zinc-finger protein family, is a common transcription factor, which is involved in several cellular responses such as cell proliferation, differentiation, metabolism, and immunity, especially in the process of adipogenesis. In order to explore the insertion/deletion (indel) genetic variations of ZNF395 and evaluate their effects on sheep growth traits, a total of 1934 samples from five Chinese and two Mongolian sheep breeds were investigated. Genetic variation analysis of ZNF395 revealed a novel 23 bp indel at the intron 3 with three genotypes. Read More

Anim Biotechnol 2019 Mar 18:1-6. Epub 2019 Mar 18.

a Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences , Lincoln University , Lincoln , New Zealand.

The study investigated the effect of stage of lactation and Breeding Worth (BW) index on estimated dry matter intake (DMI), milk solids (MS) production, energy use efficiency (EUE) and feed conversion efficiency (FCE) of grazing cows. Two hundred crossbred cows with similar calving date (14 August ± 9.97 days), live weight (471. Read More

Anim Biotechnol 2019 Mar 12:1-8. Epub 2019 Mar 12.

a Division of Animal Genetics , ICAR-Indian Veterinary Research Institute , Bareilly , UP , India.

The present study aimed to evaluate the efficiency of two Bovine SNP genotyping arrays (i.e., 50 K and HD) for breed clustering and stratification related studies in taurine and indicine breeds. Read More

Anim Biotechnol 2019 Mar 12:1-6. Epub 2019 Mar 12.

a Department of Veterinary Physiology , C.V.Sc & A.H., O.U.A.T , Bhubaneswar , India.

The present study was aimed to document the effect of heat stress on the transcriptional abundance of heat shock protein 70 (HSP70) mRNA in cultured cardiac cells of goat. The heart tissues (n = 6) from different goats were used for the culture study. The cardiac cells obtained from different heart tissues were cultured in 24 well cell culture plates and incubated in a humidified CO (5%) incubator at 37 °C. Read More

Anim Biotechnol 2019 Mar 4:1-6. Epub 2019 Mar 4.

a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.

Aldehyde dehydrogenase 1 (ALDH1) and hepatocyte nuclear factor 4A (HNF4A) are the putative mammary stem cell markers. Tissue necrosis factor alpha (TNFA) is involved in inflammation-associated carcinogenesis and cell proliferation. In this study, the gene expression profile of ALDH1, HNF4A and TNFA of buffalo mammary tissue using real-time quantitative PCR (RT-qPCR). Read More

Anim Biotechnol 2019 Feb 23:1-8. Epub 2019 Feb 23.

a Faculty of Life Science, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery , Liaoning Normal University , Dalian , China.

The relationship between PLP2 gene and cashmere fiber quality of Liaoning cashmere goat was investigated. The sheep fibroblast cells were treated with exogenous cytokines and melatonin, independently, and RNA interference, RT-PCR and in situ hybridization were utilized for investigating the PLP2 gene regulation mechanism underlying the Liaoning cashmere growth. The results showed that the expression of PLP2 gene in the prosperous and degenerative stage is higher than that of the primary follicle, indicating that the PLP2 gene promotes the secondary follicle, wherein the gene is expressed only in the inner root sheath, suggesting its correlation to hair loss. Read More

Anim Biotechnol 2019 Feb 22:1-8. Epub 2019 Feb 22.

a Department of Biology and Ecology, Faculty of Science , University of Kragujevac , Kragujevac , Serbia.

Insect silk has been widely studied for its application in regenerative medicine. However, the data about Plodia interpunctella silk as a biomaterial and its anticancer properties are insufficient. Thus, the aim of this study was to investigate native silk as a substrate for growing normal human fibroblasts MRC-5, and test potential cytotoxic effects of the two silk extracts (with DMSO and Trypsin for sericin isolation) on HCT-116 colorectal carcinoma cells and MRC-5 fibroblasts as a control. Read More

Anim Biotechnol 2019 Feb 17:1-7. Epub 2019 Feb 17.

a ICAR-National Bureau of Animal Genetic Resources , Karnal , India.

Lactoferrin (Lf) is a multifunctional bi-lobate iron-binding glycoprotein belonging to transferrin family with a mass of approximately 80 kD. Being ubiquitously present in almost all biological secretions, it performs important biological functions. One of the earliest and very well-documented functions of Lf is the antibacterial effect against broad spectrum Gram-negative and Gram-positive bacteria. Read More

Anim Biotechnol 2019 Feb 8:1-9. Epub 2019 Feb 8.

a College of Animal Science and Technology , Hebei Agricultural University , Baoding , PR China.

Somatic cell nuclear transfer (SCNT) technology has been applied in the construction of disease model, production of transgenic animals, therapeutic cloning, and other fields. However, the cloning efficiency remains limited. In our study, to improve SCNT efficiency, brilliant cresyl blue (BCB) staining were chosen to select recipient oocytes. Read More

Anim Biotechnol 2019 Feb 7:1-7. Epub 2019 Feb 7.

a Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology , Northwest A&F University , Yangling , China.

AT motif-binding factor (ATBF1) is a transcription factor that suppresses carcinogenesis and induces neuronal differentiation, as well as it directly interacts with estrogen receptor (ER) and regulates progesterone receptors signaling. Thus ATBF1 gene will possibly greatly affect reproductive tratis in animals. According to our available findings, two indel (insertion/deletion) variants (12-bp indel; 6-bp indel) within the ATBF1 gene were found in Shaanbei White cashmere goats (SWCG). Read More

Anim Biotechnol 2019 Feb 5:1-6. Epub 2019 Feb 5.

a Institute of Cellular and Molecular Biology, College of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , P. R. China.

Perilipin 2 (PLIN2) is a cytosolic protein that regulates intracellular lipid storage and mobilization. However, research reports of the relationship between PLIN2 gene and growth traits in cattle are rare. Here, five novel single nucleotide polymorphisms (SNPs)（g. Read More

Anim Biotechnol 2019 Feb 5:1-7. Epub 2019 Feb 5.

a Animal Genetics Division , ICAR-National Bureau of Animal Genetic Resources , Karnal , Haryana , India.

Brucellosis is the most dreadful disease among bovines, although breed differences have been observed in prevalence of disease, worldwide. In present study, antibody response and relative expression of proinflammatory cytokines was compared in Bos indicus (zebu) and Bos taurus × Bos indicus (crossbred) cattle vaccinated by live attenuated Brucella abortus S19 antigen. Six female calves (4-6 months age) of both groups were vaccinated with B. Read More

Anim Biotechnol 2019 Jan 28:1-6. Epub 2019 Jan 28.

a Institute of Animal Science and Veterinary Medicine , Shandong Academy of Agricultural Sciences , Jinan , Shandong , China.

Growth traits are mainly determined by genetic factors. SIRT4, a class II sirtuin, predominantly acts as an ADP-ribosyltransferase and inhibits fatty acid oxidation. In this study, a total of 1005 cattle belonging to five indigenous Chinese breeds were used to evaluate the relationship between the potential insertions/deletions (indels) within the SIRT4 gene and growth traits. Read More

Anim Biotechnol 2019 Jan 17:1-8. Epub 2019 Jan 17.

a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi 712100 , P. R. China.

Paired-like homeodomain 2 (PITX2), a key gene in hypothalamic-pituitary-adrena axis, influences animal growth and development. The objective of this study was to identify the association of the functional genetic variations within goat PITX2 gene with growth traits and mRNA expression levels. According to the reported single nucleotide polymorphisms (SNPs) information in Guanzhong dairy goat (GZDG), we identified genotypes of the known SNPs in Hainan black goat (HNBG). Read More

Anim Biotechnol 2019 Jan 14:1-6. Epub 2019 Jan 14.

b College of Veterinary Medicine , Jilin University , Changchun , China.

Melanocyte stimulating hormone receptor (MC1R) has been known as a regulator of eumelanin and phaeomelanin production in the melanocytes, and MC1R mutations causing coat color changes are known in many vertebrates; however, there are no research reports about the differentially expression of MC1R gene and its coding protein in Cashmere goats with different coat color. We examined the presence of MC1R distribution and MC1R protein and gene expression in the white Cashmere goats and black Cashmere goats, respectively; q-PCR, Western blot and immunhistochemical analysis showed that the expression of the MC1R gene in the black Cashmere goats was 3.39 fold more than the white ones (p < 0. Read More

Anim Biotechnol 2019 Jan 14:1-9. Epub 2019 Jan 14.

a Chongqing Academy of Animal Sciences , Rongchang , People's Republic of China.

The immunophilins are an important group of regulatory molecules in the immune system. FKBP5, expressed throughout mammals and in fish and birds, functions in both physiological and pathogenic pathways, including innate immunity and steroid-based diseases. In this study, we cloned the first porcine FKBP5 from Rongchang pig by the rapid amplification of cDNA ends technique. Read More

Anim Biotechnol 2019 Jan 11:1-13. Epub 2019 Jan 11.

a College of Animal Science & Veterinary Medicine , Shenyang Agricultural University , Shenyang , P. R. China.

Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200 nt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber. Read More

Anim Biotechnol 2019 Jan 5:1-7. Epub 2019 Jan 5.

a College of Animal Science and Technology , Northwest A&F University , Yangling , P.R. China.

Lysine demethylase 4D (KDM4D) encodes a histone demethylase, which can influence the androgen signaling as well as play an essential role in spermatogenesis. Recently a study has shown that the mRNA of KDM4D is the key to the successful clone of the Macaque monkeys named as "Zhongzhong" and "Huahua," which greatly increased pregnancy rates of female monkey, suggesting that the KDM4D gene may be strongly associated with reproduction. Therefore, the objective of this study was to explore possible single nucleotide polymorphisms (SNPs) within the coding region of KDM4D gene and analyze the associations with testis morphology traits of male pigs. Read More

Anim Biotechnol 2019 Jan 5:1-7. Epub 2019 Jan 5.

a School of Applied Animal Production and Biotechnology (SAAPBT), College of Veterinary and Animal Sciences , Kerala Veterinary and Animal Sciences University , Thrissur , Kerala , India.

Recognition of deleterious non-synonymous single nucleotide polymorphism (SNPs) aids in the assessment of genetic basis of diseases and prediction of clinical phenotypes. In this study, data obtained from whole exome sequencing of Vechur cow using Illumina HiSeq 2500 platform is compared with that of crossbred cattle of Kerala. Sequence analysis of selected 18 mastitis resistant genes, evaluated the consequence of non-synonymous SNPs in these genes from both Vechur and crossbred cattle of Kerala, using sequence and structure-based computational tools such as SIFT, PROVEAN and I-MUTANT 2. Read More

Anim Biotechnol 2019 Jan 2:1-7. Epub 2019 Jan 2.

a Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , Sichuan , P.R. China.

Skeletal muscle satellite cells (SMSCs) provide a good model for the study of postnatal muscle growth and intramuscular adipogenesis because they have myogenic and adipogenic differentiation ability. In this study, we harvested goat SMSCs using pronase digestion method. Then, the cells were purified from stratified liquids between 40 and 90% percoll. Read More

Anim Biotechnol 2019 Jan 2:1-9. Epub 2019 Jan 2.

a Sichuan Animal Science Academy , Chengdu , Sichuan , China.

Objective: The goal of this study was to investigate the mechanisms of muscle growth and development of three chicken breeds.

Participants: Eighteen chickens, including three different breeds with different growth speeds (White Broiler, Daheng, and Commercial Layers of Roman), were used.

Methods: Total RNA from breast muscle of these chickens was subjected to a gene expression microarray. Read More

Anim Biotechnol 2018 Dec 31:1-6. Epub 2018 Dec 31.

b Malopolska Biotechnic Center Ltd , Krasne , Poland.

The present study was aimed to determine the effect of GnRH analog (buserelin acetate) on the quality of bovine spermatozoa stored at 16° C for 24 h. Semen collected in the summer season from June to September from healthy Polish Holstein-Friesian bulls. Ejaculates were centrifuged, divided and diluted to the final concentration of 240 × 10 spermatozoa/mL using animal protein-free commercial BIOXcell extender (IMV Technologies, L'aigle, France) (Control) or with BIOXcell extender supplemented with buserelin acetate and stored 0, 8 and 24 h. Read More

Anim Biotechnol 2018 Dec 31:1-6. Epub 2018 Dec 31.

a Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education , Huazhong Agricultural University , Wuhan , P. R. China.

Spermatogenesis is a complex process regulated by many genes. In this study, H2AFZ, RNF4 and NR4A1 genes were selected as candidate genes for boar semen quality traits based on their functions during spermatogenesis, and the associations of three loci (H2AFZ c.192 + 210-192 + 213delCGAT, RNF4 c. Read More

Anim Biotechnol 2018 Dec 31:1-10. Epub 2018 Dec 31.

a Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University , Xuzhou , PR China.

The objective of this study was to examine the association of transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) genes polymorphisms with growth traits in three Chinese cattle breeds (Jiaxian red cattle, Qinchuan cattle and Luxi cattle). Through experiments we identified three single nucleotide polymorphisms (SNPs) in these three cattle breeds TRPV1 and TRPA1 genes using PCR-SSCP, (forced) PCR-RFLP methods. Three of these polymorphisms are all synonymous mutation which includes (NW_003104493. Read More

Anim Biotechnol 2018 Dec 27:1-8. Epub 2018 Dec 27.

c Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture , Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing , P. R. China.

The mechanisms that dairy cattle respond to environmental stresses are very complicated. Previous research into the molecular mechanisms of mammalian heat stress has largely focused on the role of protein-coding genes and small non-coding RNAs. Recently, it has become apparent that large numbers of long non-coding RNAs transcribed from mammalian genomes play extensive roles in transcriptional regulation. Read More

Anim Biotechnol 2018 Dec 24:1-4. Epub 2018 Dec 24.

a Division of Pathology , ICAR-Indian Veterinary Research Institute , Bareilly , India.

The objective of the present study was to develop a rapid, simple, specific and sensitive Taqman-based real-time PCR assay for porcine sapelovirus (PSV) detection. Specific primers and probe were designed from the five untranslated regions (UTRs) of the viral genome. The detection limit of the real-time PCR was 10 copies. Read More

Anim Biotechnol 2018 Dec 24:1-6. Epub 2018 Dec 24.

b Department of Medicinal Chemistry , College of Pharmacy, University of Sharjah , Sharjah , UAE.

Recombinant BK virus (rBKV) is able to express polypeptides under control of its native BKV late promoter. This ability helps to use this construct as a good reporter since it can infect human cells. In this study, we generate a BKV construct containing Renilla luciferase (Rluc) sequences under control of the BKV late promoter. Read More

Anim Biotechnol 2018 Dec 20:1-10. Epub 2018 Dec 20.

a Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling Shaanxi , People's Republic of China.

Cellular retinoic acid binding protein 2 (CRABP2) is essential to myoblast differentiation. However, there was no report about the function of CRABP2 gene in cattle. This study explored the association of CRABP2 gene polymorphisms with growth traits in cattle breeds by several methods, such as DNA sequencing, PCR, PCR-RFLP and forced PCR-RFLP. Read More

Anim Biotechnol 2018 Dec 20:1-5. Epub 2018 Dec 20.

a Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology , China Agricultural University , Beijing , P.R. China.

Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) is among the many candidate genes for regulating milk production traits in dairy cattle that have been identified via quantitative trait locus (QTL) mapping and genome-wide association studies (GWAS). Our previous studies confirmed that a G-to-A mutation at chr14:2553998 is the main cause of GPIHBP1-related effects on milk fat content. In this study, we discovered that GPIHBP1 may be a strong candidate gene for the regulation of milk protein traits. Read More

Anim Biotechnol 2018 Dec 20:1-10. Epub 2018 Dec 20.

a Department of Bioindustrial Technologies , Konkuk University , Seoul , Republic of Korea.

PERV is a major virus concerning xenotransplantation study. However, the interesting part is that PERV is present in all kinds of pigs without pathogenicity and immune response. Furthermore, since pig cells have receptors for PERV, the gene delivery system using PERV envelope is highly likely to develop into an excellent viral vector in pigs. Read More

Anim Biotechnol 2018 Dec 20:1-14. Epub 2018 Dec 20.

a Fish Genetics and Biotechnology Division , ICAR-Central Institute of Fisheries Education (CIFE) , Mumbai , Maharashtra , India.

The 17-beta-hydroxysteroid dehydrogenase 2 (17β-HSD2) enzyme regulates steroid levels by the inactivation of estrogen and androgens. Spermatogenesis associated protein 2 (SPATA2) plays a vital role in spermatogenesis in vertebrates including fish. We report cloning and characterization of full cds of 17β-HSD2 and SPATA2 genes in Clarias magur. Read More

Anim Biotechnol 2018 Dec 20:1-8. Epub 2018 Dec 20.

a School of Life Science and Technology , Southwest Minzu University , Chengdu , P.R. China.

GTP binding protein overexpressed in skeletal muscle (GEM) is an important gene with many functions, such as regulating the rearrangement of cytoskeleton and the activity of voltage-dependent calcium channel, and GEM was regarded as a candidate gene for obesity. However, little investigation has been carried out to explore whether GEM affected the intramuscular fat (IMF) deposition of goat. To explore the role of GEM gene in goat, this gene was cloned and its tissue and temporal expression profile were detected. Read More

Anim Biotechnol 2018 Dec 6:1-9. Epub 2018 Dec 6.

b Wildlife Institute of India , Dehradun , India.

Musk deer are of high conservation priority owing to poaching pressure because of its musk pod. Representation of musk deer status using genetics is poorly documented in India, and it is not confirmed as to how many species of musk deer are present. We characterize for the first time, the genetic diversity of musk deer from Uttarakhand using Cytochrome Oxidase sub-unit (COI) gene (486 bp) and compared with the data available for other species. Read More

Anim Biotechnol 2018 Dec 6:1-6. Epub 2018 Dec 6.

a Swine Science Division , National Institute of Animal Science , Cheoan-si , Republic of Korea.

Tissue plasminogen activator (tPA) is a protein involved in the breakdown of blood clots. We have previously produced a human tPA (htPA)-overexpressing transgenic pig using a mammary gland-specific promoter. In this study, we have established a transgenic pig mammary gland cell line that produces recombinant htPA. Read More

Anim Biotechnol 2018 Nov 30:1-9. Epub 2018 Nov 30.

a College of Life Science and Technology , Southwest Minzu University , Chengdu , China.

The aim of this study was to clone KLF6 gene of Tibetan chicken, clarify its temporal-spatial expression characteristics, and build the correlation between the expression level of KLF6 gene and IMF content in different developmental stages. RT-PCR was used to clone Tibetan chicken KLF6 gene, qPCR was used to detect the expression level of KLF6 gene in different tissues and developmental stages. The sequence of KLF6 gene was 919 bp including a complete 852 bp CDS region. Read More

Anim Biotechnol 2018 Nov 30:1-8. Epub 2018 Nov 30.

a Department of Animal Reproduction Biotechnology , National Research Institute of Animal Production , Kraków , Poland.

The objective of the present study was to evaluate the effect of porcine Mesenchymal Stem Cells (MSCs) secreted factors on bovine in vitro embryo development by using MSCs in different culture systems: SOF medium, SOF medium conditioned by MSCs in monolayer and in reverse drop and by embryo culture in co-culture with MSCs. Statistically highly significant differences were noted between the number of blastocysts derived cultures in all tested culture systems. The in vitro culture in SOF turned out to be the most optimal. Read More

Anim Biotechnol 2018 Nov 24:1-8. Epub 2018 Nov 24.

a College of Animal Science and Veterinary Medicine , Henan Agricultural University , Zhengzhou , China.

Previous studies have revealed a significant association between SNPs found within the heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) gene and obesity. This study identified a novel 43-bp indel polymorphism in intron 1 of HS6ST3 in 1963 chickens from nine different breeds, and three genotypes, designated II, ID and DD, were observed. The frequency of the 'I' (0. Read More