Search Our Scientific Publications & Authors

Anim Biotechnol 2020 May 27:1-7. Epub 2020 May 27.

College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.

In our study, four single nucleotide polymorphisms (SNPs) were identified in exon 2 of () gene in 488 Chinese Qinchuan (QC) cattle, which included two missense mutations T 2084G and G 2107C, two synonymous mutations T 2052C and T 2169C. Further, we evaluated haplotype frequency and linkage disequilibrium (LD) coefficient of four SNPs. At SNP T 2052C, G 2107C and T 2169C, the QC cattle population belonged to intermediate genetic diversity (0. Read More

Anim Biotechnol 2020 May 19:1-10. Epub 2020 May 19.

Department of Animal Breeding and Genetics, Orissa University of Agriculture and Technology, Bhubaneswar, India.

The Caprine Prion Protein (PrP) gene polymorphism in three different native Indian goat populations of Southern Odisha, namely Ganjam (a registered breed of India), Ghumusari and Raighar was studied. The 876 bp amplified segment of PrP gene contains full length coding sequence of 771 bp. In Ganjam and Ghumusari goats, any difference of nucleotide sequence was not identified. Read More

Anim Biotechnol 2020 May 14:1-8. Epub 2020 May 14.

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.

MicroRNAs (miRNAs) are a class of conserved non-coding RNAs that are widely regarded as important regulators in a variety of biological processes. Increasing evidence has revealed that skeletal muscle fiber-type conversion is regulated by miRNAs, but the molecular mechanism is still not fully understood. In this study, we confirmed the role of miR-22-3p on skeletal muscle fiber-type conversion and investigated its potential mechanism in C2C12 myotubes. Read More

Anim Biotechnol 2020 May 13:1-10. Epub 2020 May 13.

College of Animal Science, Yangtze University, Jingzhou, Hubei, China.

Isoflavones possess a wide range of physiological effects. However, it is still unclear whether isoflavones can promote milk synthesis in mammary gland. This study aimed to determine the effects of a main soy isoflavone, daidzein, on milk synthesis and proliferation of mammary epithelial cells (MECs) and reveal the underlying molecular mechanism. Read More

Anim Biotechnol 2020 May 13:1-9. Epub 2020 May 13.

College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi, Xinjiang, China.

In our previous genome-wide association study (GWAS), we identified the fragile histidine triad diadenosine triphosphatase () gene in Xinjiang brown cattle (XJBC) as a candidate gene associated with cattle productive traits, with potential application in mark-assisted selection (MAS) in cattle breeding. is a prototype of a class of tumor suppressor genes that contain genomic loci mapped to common fragile loci. Here, 388 healthy and unrelated XJBC were selected to identify insertion/deletion (InDel) variants in the bovine and assess their effects on milk traits. Read More

Anim Biotechnol 2020 May 5:1-12. Epub 2020 May 5.

College of Animal Science and Technology, Northwest A&F University, Yangling, China.

(prosaposin) is widely expressed in different organs, and plays an important role in fat deposit. Insertion/Deletion (InDel) is a relatively simple and effective DNA marker. However, the association of molecular marker at different stages of animal development has not received enough attention, especially fat deposition related traits. Read More

Anim Biotechnol 2020 May 5:1-21. Epub 2020 May 5.

Fish Health Management Division, Immunology Laboratory, Indian Council of Agricultural Research-Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar, Odisha, India.

Myxovirus resistance (Mx) proteins belonging to the dynamin superfamily of high molecular weight GTPases exist in various isoforms and play crucial role in innate immunity. In addition to the isoforms, Mx1 also plays important role in exerting its anti-viral actions against a broad range of animal RNA viruses. In rohu (, 1 full-length cDNA sequence consists of 2440 nucleotides (nt) encoding 628 amino acids (aa) polypeptide of 71. Read More

Anim Biotechnol 2020 May 2:1-9. Epub 2020 May 2.

Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, Korea.

Folic acid (FA) is known to be an important micronutrient in humans; however, information regarding the effect of FA supplementation on bovine mammary epithelial (BME) cells is insufficient. FA supplementation is reported to increase milk production in dairy cows, but the underlying molecular mechanisms are unknown. This study examined the effects of FA supplementation on the proliferation and apoptosis of a BME cell line (MAC-T). Read More

Anim Biotechnol 2020 Apr 27:1-12. Epub 2020 Apr 27.

Department of Animal Biotechnology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, Republic of Korea.

Satellite cells promote muscle repairing and muscle growth. Thereby the intention of the present study was to investigate the beneficial effects of heat stress at different time intervals on chicken satellite cells' viability. Satellite cells were isolated from 1-day-old chicks and treated at two different temperatures (37 °C and 41 °C) for various time periods (6 h, 12 h, 24 h, 48 h, and 72 h). Read More

Anim Biotechnol 2020 Apr 23:1-8. Epub 2020 Apr 23.

Key Laboratory of Animal Genetics and Breeding of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China.

This study was aimed to establish a highly specific and sensitive loop-mediated isothermal amplification (LAMP) method for diagnosing avian infectious laryngotracheitis (AILT). DNA was extracted from isolated infectious laryngotracheitis virus (ILTV) strains and control samples, followed by PCR using three sets of six specific primers. The detection efficiency of the LAMP assay was evaluated by the turbidity and calcein methods. Read More

Anim Biotechnol 2020 Apr 17:1-8. Epub 2020 Apr 17.

Department of Animal Sciences, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland.

This experiment evaluated the effect of Pro-Biotyk (Em-15) and EMFarma™ probiotics on body weight, feed intake and conversion, carcass traits, and microbial contamination in a poultry house. The probiotic preparations caused a nonsignificant increase in body weight (42 days), feed intake, and feed conversion ratio (1-42 days) and a nonsignificant decrease in chicken mortality from 4 weeks of rearing. Chickens exposed to probiotics did not differ significantly in preslaughter body weight, carcass weight, dressing percentage, and the content of carcass components. Read More

Anim Biotechnol 2020 Apr 15:1-10. Epub 2020 Apr 15.

Livestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of Gabès, Médenine, Tunisia.

Healthy animals can constitute a reservoir for potentially dangerous for humans. Our objectives were to investigate virulence genes in isolated from healthy animals in southern Tunisia and to determine their resistance to antimicrobials of high importance in humans and animals. 126 fecal samples were collected from healthy animals (cattle, sheep, goats, chicken, camel, bustard and rabbit) and assayed by PCR for virulence genes and by disk diffusion for antimicrobial resistance. Read More

Anim Biotechnol 2020 Apr 15:1-8. Epub 2020 Apr 15.

Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.

Prolactin is a highly versatile pituitary hormone with multiple biological functions. expression is regulated by and the prophet of (). The aim of this study was to investigate the indel variations in ovine and the directly related ( and ) genes, and their associations with growth traits in Luxi Blackhead (LXBH) sheep. Read More

Anim Biotechnol 2020 Apr 15:1-6. Epub 2020 Apr 15.

College of Animal Science and Technology, Southwest University, Chongqing, China.

This study aims to identify the relative Copy number variation (CNV) associated with the litter size of Dazu black goats based on the unpublished CNV analytical results of our previously published sequencing data, in which the litter-size groups were classified into extreme low- and high-yield groups. Firstly, to compare the existence of valuable CNV in Dazu black goats with different fertility levels with mixed pools. We obtained 4992 and 4888 CNVs from the HY and LY, which overlapping 1461 genes, and classified on the original CNV type. Read More

Anim Biotechnol 2020 Apr 15:1-8. Epub 2020 Apr 15.

ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India.

This study aimed to assess the effect of the insulin-like grow factor 1 (IGF-1) treatment during maturation on the gene expression and developmental ability of ovine oocytes. Ovine cumulus-oocyte complexes (COC) were matured without (control) or with the supplementation of IGF-1 (100 ng/ml) and then subjected to fertilization and culture. The rate of oocyte maturation and embryo development was recorded and expression of the selected genes (involved in the PI3K/Akt and apoptosis signaling) was assessed in the matured oocytes. Read More

Anim Biotechnol 2020 Apr 4:1-11. Epub 2020 Apr 4.

Department of Animal Production, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

The assay aimed to evaluate the effect of feeding a recently developed phytogenic feed additives mixture in diets of lactating Friesian cows ( = 30; 514 ± 10.1 kg body weight) for 3 months. Cows were stratified into three groups of 10 cows each and fed a control diet alone or the control diet supplemented with the additives mixture at 3 g (PHY3) or 6 g (PHY6)/cow daily. Read More

Anim Biotechnol 2020 Apr 4:1-7. Epub 2020 Apr 4.

Animal and Poultry Production, Desert Research Center, Mataria, Egypt.

Traditionally, sheep are raised in Egypt for meat and wool production. Selection for higher wool quality could increase its suitability for particular processing procedures in the wool industry, which maximizes the profit of the sheep production enterprise. In this research, the effect of genetic polymorphisms of the keratin-associated protein 6-1 (KAP6-1) on wool traits was investigated in Barki sheep. Read More

Anim Biotechnol 2020 Apr 4:1-9. Epub 2020 Apr 4.

PpBioNut Laboratory, University Abou Bekr Belkaid, Tlemcen, Algeria.

Cattle plays a very important role in agriculture and food security in Algeria. In the present study, the genetic diversity and structure of Algerian indigenous cattle populations were evaluated by microsatellite markers. A total of 138 individuals belonging to four cattle breed populations were characterized using 22 microsatellite markers. Read More

Anim Biotechnol 2020 Apr 2:1-11. Epub 2020 Apr 2.

Division of Biological Standardization, ICAR-Indian Veterinary Research Institute, Bareilly, India.

The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of strain 544 (S544) in the spleen of mice for potency testing of live strain 19 (S19) vaccine. and gene-based qPCR were optimized for calculating copy number. The copy number was further correlated with live count in the spleen by standard plate count (SPC) method. Read More

Anim Biotechnol 2020 Apr 1:1-14. Epub 2020 Apr 1.

College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, P.R. China.

Long non-coding RNAs (lncRNAs) is a class of eukaryotic transcripts with length of more than 200 bp. They contribute to the regulation of gene expressions involved in multiple processes including the skin cell proliferation, differentiation, and reconstruction of the secondary hair follicles (SHFs). In this study, firstly, we identified 16 putative lncRNAs from SHFs of cashmere goat based on the EST sequences from NCBI database. Read More

Anim Biotechnol 2020 Mar 26:1-11. Epub 2020 Mar 26.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.

Cattle fatty liver has caused mass damage in milk production during the past few years. In our study, to identify different miRNAs involved in cell physiological regulation in fatty liver, we performed miRNA deep sequencing on a normal liver cell (S01), fatty liver cell (S02) and processed cell by monoammonium glycyrrhizinate (S03). As a result, a total of 15,277,462, 14,190,360 and 13,771,060 raw reads representing 13,904,074, 12,784,128 and 11,017,604 clean reads per library were obtained separately. Read More

Anim Biotechnol 2020 Mar 25:1-5. Epub 2020 Mar 25.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.

Copy number variation (CNV) has been used as an important source of phenotypic and genetic diversity in recent years. Nicastrin () gene is usually attached to human diseases such as Alzheimer's disease, and Acne inversa. However, there are no essays about the gene combining with cattle breeds. Read More

Anim Biotechnol 2020 Mar 18:1-9. Epub 2020 Mar 18.

Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA.

is a pathogen normally found in the gastrointestinal tract of poultry. The objective of this study was to determine changes in avian β-defensin (AvBD) and liver-enriched antimicrobial peptide 2 (LEAP2) mRNA following challenge. Day of hatch chicks were challenged with 10, 10 or 10 colony-forming units (cfu) of . Read More

Anim Biotechnol 2020 Mar 17:1-7. Epub 2020 Mar 17.

College of Veterinary Medicine, Yangzhou University, Yangzhou, PR China.

This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma concentrations of altrenogest (AT) were measured by high performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) and the concentration-time data of AT was analyzed by WINNONLIN 5. Read More

Anim Biotechnol 2020 Mar 17:1-7. Epub 2020 Mar 17.

Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, P. R. China.

Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck (). The full-length cDNA sequence of the gene was cloned via rapid amplification of cDNA ends. Read More

Anim Biotechnol 2020 Mar 14:1-11. Epub 2020 Mar 14.

College of Life Sciences, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang, P. R. China.

Spider silk, which has remarkable characteristics, has wide application prospects in many fields. Many researchers have explored potential methods for directly producing spider silk proteins and spidroins with mechanical properties or obtaining recombinant spider silk fibers by genetic engineering methods. However, there are still some shortcomings with these methods, such as inability to simulate the fibrosis process of spider silk. Read More

Anim Biotechnol 2020 Mar 13:1-10. Epub 2020 Mar 13.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China.

Goat milk in some cases is less allergenic than cow milk, therefore, more people drink goat milk in the world, so it is necessary for us to improve the yield and quality of goat milk. Previous studies have shown that some genes are closely related to lactation. Ovarian cancer G protein-coupled 1 (OGR1) is a G protein-coupled receptor discovered recently. Read More

Anim Biotechnol 2020 Mar 12:1-8. Epub 2020 Mar 12.

College of Life Science and Technology, Lingnan Normal University, Zhanjiang, Guangdong, China.

In this study, embryos of from eye primordium formation to the larval growing stage were collected and used for RNA-Seq analysis. A total of 183,542,186 clean reads were assembled de novo into 58,054 unigenes consisting of 54,118,228 bp, with the average length at 932 bp and the N50 at 1667 bp. 21,469 (36. Read More

Anim Biotechnol 2020 Mar 12:1-5. Epub 2020 Mar 12.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Liangshan cattle are a very small indigenous breed with adult weight of less than 300 Kg and have been mainly distributed in the Liangshan Yi Autonomous Prefecture of Southwestern Sichuan, China. Due to its long-term adaptation to local environments, Liangshan cattle is a valuable genetic resource and should be paid with more attentions. However, the genetic diversity of Liangshan cattle have not been specifically investigated yet, which would be required when designing the appropriate conservation and utilization programs. Read More

Anim Biotechnol 2020 Mar 12:1-8. Epub 2020 Mar 12.

Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, India.

The objective of the present study was to investigate the epidemiological and haematological parameters with simultaneous molecular detection of infection of crossbred jersey (CBJ) cattle. Haematological values like mean hemoglobin (Hb) (7.31 ± 2. Read More

Anim Biotechnol 2020 Mar 10:1-6. Epub 2020 Mar 10.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.

Myostatin () gene, also known as () gene, is a negative regulator of skeletal muscle growth and development, especially the number, size and type of muscle fibers. Its mutations contribute to the double-muscling (DBM) phenomenon which significantly increases the muscle mass. Hence, variations within / gene receive so much attention in several kinds of species such as bovines, poultries, goats, sheep, horses. Read More

Anim Biotechnol 2020 Mar 10:1-7. Epub 2020 Mar 10.

Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran.

Medicinal plants and their derivatives are human-friendly, nutritional supplements, and their use in the poultry industry is increasing today. is a very high-quality antioxidant medicine plant that has recently attracted the attention of poultry and nutrition researchers to supplement their rations and reduce feed costs. In the present study, the effect of powder and extract of on broilers has reviewed. Read More

Anim Biotechnol 2020 Mar 5:1-8. Epub 2020 Mar 5.

Key Laboratory for Animal Disease-Resistance Nutrition of China, Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, P. R. China.

This study aimed to investigate effects of apple polyphenols (APPs) on myofiber-type transformation in muscle of finishing pigs and its mechanism. In this study, 36 healthy castrated Duroc × Landrace × Yorkshire pigs with an average body weight of 71.25 ± 2. Read More

Anim Biotechnol 2020 Feb 27:1-11. Epub 2020 Feb 27.

School of Life Science and Technology, Southwest Minzu University, Chengdu, China.

Retinoid X receptor α (α) is thought to be a key regulator in lipid metabolism, glucose metabolism, adipogenic differentiation, gene expression, and inflammatory response. However, it is not clear whether α has any role in intramuscular preadipocyte of goat. In the current investigation, we report that adenovirus overexpression of α promotes lipid accumulation in intramuscular adipocyte and up-regulates the expression of positive indicator genes (γ and 4). Read More

Anim Biotechnol 2020 Feb 24:1-7. Epub 2020 Feb 24.

College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China.

Ningqiang pony is one of the five pony breeds in China and is listed as a rare species. It is, therefore, meaningful to conduct stature research on this breed for its efficient reproduction, utilization and protection. This study is based on four SNP variants of adjacent LCORL/NCAPG, HMGA2, ZFAT and LASP1 genes, which have been found to be associated with horse height, and then, the 4 SNP loci in 22 Ningqiang ponies were analyzed. Read More

Anim Biotechnol 2020 Feb 20:1-7. Epub 2020 Feb 20.

College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China.

Coat color is one of the major quality traits of animals, and miR-129-5p acts as an important regulator for melanin biosynthesis in mammals. In this study, real-time PCR and western blotting were used to examine the expression of miR-129-5p and its targets genes in the skin of different coat color goats. The results showed that the expression of miR-129-5p in the skin samples of Inner Mongolia cashmere goats (IMCG) was higher than that of Dazu black goat (DBG). Read More

Anim Biotechnol 2020 Feb 18:1-6. Epub 2020 Feb 18.

Department of Animal Breeding, Genetics and Biostatistics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Thrissur, India.

The NAD-dependent protein deacetylase, Sirtuin3 (SIRT3), plays a role in fertility by preventing the activation of reactive oxygen species (ROS). A novel study was conducted on caprine , to study its ovarian expression, explore the sequence variability in exon 7 and analyze its association with prolificacy in two native goat breeds of Kerala, Malabari and Attappady Black. The mRNA isolated from ovaries of six Malabari and Attappady Black goats were subjected to quantitative PCR (qPCR) using and as reference genes. Read More

Anim Biotechnol 2020 Feb 14:1-9. Epub 2020 Feb 14.

Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.

This experiment was carried out to study the effect of water extracted pomegranate peel extract (PE) on ruminal protein degradation and post-ruminal digestion in the dairy cow. PE was added at six levels of total phenolics (g/kg of the basal diet); 3.75 (PE1); 4. Read More

Anim Biotechnol 2020 Feb 13:1-5. Epub 2020 Feb 13.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

The production traits of cattle, especially milk trait, are of great significance to human life. A quantitative trait loci (QTL) associated with milk fat content was detected in the centromeric region of cattle chromosome 14. This QTL harbors a strong candidate gene called responsible for the milk quality. Read More

Anim Biotechnol 2020 Feb 12:1-7. Epub 2020 Feb 12.

Swine Science Division, National Institute of Animal Science, Cheonan-si, Republic of Korea.

A synthetic progestin altrenogest (ALT) is used to synchronize the estrus cycle of swine for fixed-time artificial insemination (AI) and has been shown to improve follicular development and reproductive performances in post-weaning sows. However, the effects of ALT treatment on reproductive tracts, including the ovaries, oviducts and uterus have not been yet clarified. In this study, we examined the expression of genes involved in endometrial responses in ALT-treated sows. Read More

Anim Biotechnol 2020 Feb 12:1-8. Epub 2020 Feb 12.

Department of Animal Breeding and Genetics, Odisha University of Agriculture and Technology, Bhubaneswar, India.

The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar) which differ in body size, coat color and production performance were assessed for effect of thermal stress at different temperature humidity index (THI). Read More

Anim Biotechnol 2020 Feb 8:1-6. Epub 2020 Feb 8.

College of Biology and the Environment, Nanjing Forestry University, Nanjing, China.

The red-crowned crane () is one of the most endangered cranes in the world, and its wild population is still declining. To characterize the genetic resources of East Asian migratory populations, we studied the genetic variation in wild red-crowned cranes at the Yancheng reserve. Based on a partial gene sequence, 32 wild red-crowned crane samples were screened from 100 feathers with unknown sample information. Read More

Anim Biotechnol 2020 Feb 7:1-12. Epub 2020 Feb 7.

Department of Animal Genetics and Breeding, ICAR-National Dairy Research Institute, Karnal, India.

This study is the maiden attempt for genetic characterization of the Purnathadi, a phenotypically distinct buffalo population of the western Vidarbha region of Maharashtra and to explore genetic diversity using STR markers. A total of 48 unrelated Purnathadi buffaloes from the entire native tract were genotyped using a battery of 25 heterologous microsatellite markers. 5' end of forward primer of each microsatellite marker was labeled with one of the fluorescent dyes, viz. Read More

Anim Biotechnol 2020 Feb 5:1-9. Epub 2020 Feb 5.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Copy number variation (CNV) as an important source of genetic phenotypic and variation is related to complex phenotypic traits. The aim of this study was to investigate the potential associations of copy numbers variations with sheep growth traits in three Chinese sheep breeds (CKS, STHS, and HS). is located within the stature and udder attachment quantitative trait loci (QTL) in sheep. Read More

Anim Biotechnol 2020 Feb 3:1-9. Epub 2020 Feb 3.

Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.

The present study in sheep model was to find out the interaction of apoptotic transcripts, that is, and and pluripotency related transcripts, that is, and in ovine embryos produced at different O concentrations (20% and 5% O) to compare their developmental potential. Oxygen concentrations did not influence the maturation and cleavage rate but the percentage of morula and blastocysts was significantly more at 5% as compared to 20% O. A significant upregulated expression of and genes and significantly downregulated expression of and were observed in the blastocysts at 5% than those at 20% O. Read More

Anim Biotechnol 2020 Jan 22:1-12. Epub 2020 Jan 22.

College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.

The Manila clam, , is an ecologically and economically important marine bivalve species. In this study, we conducted transcriptomic sequencing of two different shell color strains (O and Z) before color appearance (uncolored juvenile clam) and pigmented shell color (colored juvenile clam) and investigated the analysis of the differential expression patterns of specific genes associated with pigmentation by RNA-seq and time course qPCR analysis. The transcription level of 16 differentially expressed genes (DEGs) related with shell color was analyzed by qRT-PCR to validate the performance of RNA-seq from Illumina sequence data where most of them were up-regulated. Read More

Anim Biotechnol 2020 Jan 21:1-7. Epub 2020 Jan 21.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.

The use of inorganic copper in feed is hazardous. As probiotics of animals, can absorb copper ions and transform them to organic copper. This study aimed to domesticate the ability of (CICC 32211) to absorb and transform copper ions. Read More

Anim Biotechnol 2020 Jan 21:1-5. Epub 2020 Jan 21.

UMR85 Physiologie de la Reproduction et des Comportements, INRA, Nouzilly, France.

The depth of intravaginal insemination to achieve optimum fertility with frozen-thawed semen is highly species specific in birds and differ even in breed and/or strains of a species. Therefore, study was designed to evaluate the influence of intravaginal insemination depths (2 and 4 cm) on fertility outcome in Indian red jungle fowl. Semen collected from eight mature cocks was pooled, diluted in extender and cooled to 4 °C. Read More

Anim Biotechnol 2020 Jan 16:1-3. Epub 2020 Jan 16.

College of Life Science, Hebei Normal University, Shijiazhuang, China.

() and () are protozoan parasites that cause severe disease in animals and significant economic losses for farmers worldwide. is considered to be a serious parasite for dairy cows, while infection is a serious parasitic disease that can infect people and livestock. The aim of the present study was to investigate the seroprevalence of and in dairy cows in Hebei province, China. Read More

Anim Biotechnol 2020 Jan 10:1-7. Epub 2020 Jan 10.

Department of Environment, Animal Hygiene and Welfare, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.

The aim of this work was to determine the dependency between polymorphism in the locus g.8398G > A and fertility rates, bodyweight of cows and calves, the course of parturition and viability of calves in Polish Black-and-White Holstein-Friesian cattle. Three genotypes (AA, AG, and GG) were identified with a frequency of 0. Read More