255,497 results match your criteria Analytical sciences : the international journal of the Japan Society for Analytical Chemistry[Journal]


Preclinical evaluation of a mini-arthroplasty implant based on polyetheretherketone and Ti6AI4V for treatment of a focal osteochondral defect in the femoral head of hip.

Biomed Mater 2020 Jun 4. Epub 2020 Jun 4.

Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, 200127, CHINA.

A mini or partial arthroplasty may offer the advantages of reduced pain, shorter hospital stay, and increased range of motion, which are beneficial for the treatment of large-sized focal osteochondral defects. We aimed to evaluate the in vivo histologic response and function of our nonresorbable, composite structure implant, developed using polyetheretherketone (PEEK) and Ti6AI4V alloy, as a treatment for full-thickness osteochondral defects in the femoral head of the hip using a goat model. The gross and imaging appearance and histologic results were compared to those of a similar size cobalt-chromium-molybdenum (CoCrMo) alloy implant placed in a defect in the femoral head and evaluated up to 12 weeks. Read More

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http://dx.doi.org/10.1088/1748-605X/ab998aDOI Listing

Adenine-stabilized carbon dots for highly sensitive and selective sensing of copper(II) ions and cell imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2020 May 26;239:118531. Epub 2020 May 26.

College of Chemistry, Nanchang University, Nanchang 330031, China.

Adenine-stabilized carbon dots (A-CDs) are shown to be a viable fluorescent probe for highly sensitive detection and imaging of Cu. The probe has a linear fluorometric response in the 1-700 nM concentration range and a 0.3 nM detection limit. Read More

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http://dx.doi.org/10.1016/j.saa.2020.118531DOI Listing

Detecting Ferric Iron by Microalgal Residue-Derived Fluorescent Nanosensor with an Advanced Kinetic Model.

iScience 2020 May 18;23(6):101174. Epub 2020 May 18.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, P. R. China. Electronic address:

Biomass-derived carbon quantum dots (CQDs) are attractive to serve as fluorescent nanosensors owing to their superior environmental compatibility and biocompatibility. However, the detection range has been limited, only in partial agreement with the experimental data. Thus, an advanced kinetic model for quantifying the fluorescence quenching over a wide range is on demand. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101174DOI Listing

Wastewater-based epidemiology combined with local prescription analysis as a tool for temporalmonitoring of drugs trends - A UK perspective.

Sci Total Environ 2020 May 15;735:139433. Epub 2020 May 15.

Department of Chemistry, Faculty of Science, University of Bath, Bath BA2 7AY, United Kingdom. Electronic address:

This paper reports the application of wastewater-based epidemiology (WBE) for the monitoring of one city in the UK in years 2014-2018 as a means of 1) exploring relative temporal changes of illicit drug usage trends across 5 sampling weeks in 5 years, (2) assessing policy impact in reducing drug consumption, focussing particularly on mephedrone, which was classified as a class B drug in the UK in 2010, and the effects of subsequent regulation such as the novel psychoactive substances (NPS) bill of 2016, (3) investigating temporal changes in consumption of prescription pharmaceuticals vs illicit drug usage, and (4) comparing consumption of prescription drugs with WBE to enable more accurate verification of prescription drugs with abuse potential. Mephedrone was quantified only for the first two years of the study, 2014-2015, and remained undetected for the next three years of the study. This shows that given enough time changes in drug policy can have an effect on drug consumption. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139433DOI Listing

Mitigation of 3-MCPD esters and glycidyl esters during the physical refining process of palm oil by micro and macro laboratory scale refining.

Food Chem 2020 May 28;328:127147. Epub 2020 May 28.

Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia. Electronic address:

The reduction of the 3-monochloropropane-1,2-diol esters (3-MCPDE) and glycidyl esters (GE) was successfully achieved by the optimization of four processing parameters: phosphoric acid dosage, degumming temperature, bleaching earth dosage, and deodorization temperature by response surface methodology without the need for additional processing steps. The optimized processing conditions were 0.31% phosphoric acid dosage, 50 °C degumming temperature, 3% bleaching earth dosage, and 240 °C deodorization temperature. Read More

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http://dx.doi.org/10.1016/j.foodchem.2020.127147DOI Listing

Disentangling of the ecotoxicological signal using "omics" analyses, a lesson from the survey of the impact of cyanobacterial proliferations on fishes.

Authors:
Benjamin Marie

Sci Total Environ 2020 May 26;736:139701. Epub 2020 May 26.

Muséum National d'Histoire Naturelle, UMR 7245, CNRS, MNHN Molécules de Communication et Adaptation des Micro-organismes (MCAM), équipe "Cyanobactéries, Cyanotoxines et Environnement", 12 rue Buffon, CP 39, 75231 Paris Cedex 05, France. Electronic address:

Omics technologies offer unprecedented perspectives for the rational investigation of complex biological systems. Indeed, omics present the ability of offering an extensive perception of the biochemistry and physiology of the cell and of any perturbing consequences of contaminants through the joint investigation of thousands of molecular responses simultaneously; then it has recently conducted to a fervent attention by research ecotoxicologists. Beyond the presentation of latest advances, exemplified here by omics investigation of cyanobacterial deleterious effects on various fishes (at various experimental and biological scales and with various analytical tools and pipeline), the present review paper re-explores the promising perspectives and also the pitfalls of such holistic investigations of the ecotoxicological response of organisms for environmental assessment. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139701DOI Listing

Cadmium isotopes as tracers in environmental studies: A review.

Sci Total Environ 2020 May 21;736:139585. Epub 2020 May 21.

Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510405, China.

Cadmium isotopic compositions in non-contaminated systems and anthropogenic sources of Cd generally have different isotopic signatures. Cadmium isotopes, as a novel tracer, can be useful for fingerprinting the anthropogenic Cd sources, providing a promising source tracing technique in environmental studies. This review presents: (i) analytical techniques for Cd isotopic composition; (ii) isotopic signatures of Cd derived from anthropogenic activities; (iii) isotopic compositions of Cd in the industrial-impacted environmental samples; (iv) cadmium isotopic fractionation induced by geochemical process. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139585DOI Listing

Heat flux sensor to create a design space for freeze-drying development.

Eur J Pharm Biopharm 2020 Jun 1. Epub 2020 Jun 1.

Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilian University, D-81377 Munich, Germany.

Freeze-drying methodology requires an in-depth understanding and characterization for optimal processing of biopharmaceuticals. Particularly the primary drying phase, the longest and most expensive stage of the process, is of interest for optimization. The currently used process analytical technology (PAT) tools give highly valuable insights but come with limitations. Read More

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http://dx.doi.org/10.1016/j.ejpb.2020.05.028DOI Listing

Interactions of antithrombotic herbal medicines with Western cardiovascular drugs.

Pharmacol Res 2020 Jun 1:104963. Epub 2020 Jun 1.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, 999078, China. Electronic address:

Thrombotic events act as a critical factor that interferes with Cardiovascular Diseases (CVDs), and antithrombotic herbal medicine is a long-standing controversial issue. Although a dispute is involved in their clinical application, all parties unanimously agree that herbal products have been widely used in folk medicine, and their interactions with conventional drugs are of high concern. This study aims to investigate how antithrombotic herbal medicines interact with Western cardiovascular drugs on the molecular level by taking an example of the most frequently used herbal pair, Danshen-Chuanxiong (DS-CX), and to discover more scientific evidence on their potential herbal-drug interactions. Read More

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http://dx.doi.org/10.1016/j.phrs.2020.104963DOI Listing

Assessment of pre-analytical sample handling conditions for comprehensive liquid biopsy analyses.

J Mol Diagn 2020 Jun 1. Epub 2020 Jun 1.

CCRI, Children's Cancer Research Institute, St. Anna Kinderkrebsforschung, 1090 Vienna, Austria; Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria. Electronic address:

Liquid biopsies as minimally invasive approach have the potential to revolutionize molecular diagnostics. Yet while protocols for sample handling and the isolation of circulating tumor DNA (ctDNA) are numerous, comprehensive guidelines for diagnostics and research considering all aspects of real-life multi-center clinical studies are currently not available. These include limitations in sample volume, transport, and blood collection tubes. Read More

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http://dx.doi.org/10.1016/j.jmoldx.2020.05.006DOI Listing

The relationship between readiness for treatment of substance use and self-efficacy based on life skills.

J Ethn Subst Abuse 2020 Jun 4:1-13. Epub 2020 Jun 4.

Sabzevar University of Medical Sciences, Sabzevar, Iran.

Substance use is a social harm. Promoting substance users readiness for treatment of substance use with a non-medical approach is important besides the medical approach. The current study aimed to determine the relationship between readiness for treatment of substance use and self-efficacy based on life skills. Read More

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http://dx.doi.org/10.1080/15332640.2020.1772930DOI Listing

Multiple Vacancies on (111) Facets of Single-Crystal NiFe2O4 Spinel Boost Electrocatalytic Oxygen Evolution Reaction.

Chem Asian J 2020 Jun 4. Epub 2020 Jun 4.

Qingdao University, College of Chemical and Environmental Engineering, 308 Ningxia Road, 266071, Qingdao, CHINA.

Oxygen evolution reaction (OER) as the rate-determining reaction of water splitting has been attracting enormous attention. As present, only some noble-metal oxide materials (IrO 2 and RuO 2 ) have been reported as efficient OER electrocatalysts for OER. However, the high cost and scarcity of these noble-metal oxide materials greatly hamper their large-scale practical application. Read More

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http://dx.doi.org/10.1002/asia.202000468DOI Listing

Demystifying NMR Spectroscopy: Applications of Benchtop Spectrometers in the Undergraduate Teaching laboratory.

Magn Reson Chem 2020 Jun 4. Epub 2020 Jun 4.

Institute of Chemical Sciences, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, United Kingdom, EH14 4AS.

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http://dx.doi.org/10.1002/mrc.5055DOI Listing

The Earlier the Better: Structural Analysis and Separation of Lanthanides with Pyrroloquinoline Quinone.

Chemistry 2020 Jun 4. Epub 2020 Jun 4.

Ludwig-Maximilians-Universität München, Department of Chemistry, Butenandtstr. 5-13, 81377, München, GERMANY.

Lanthanides (Ln) are critical raw materials, however, their mining and purification have a considerable negative environmental impact and sustainable recycling and separation strategies for these elements are needed. In this study, we present the precipitation and solubility behavior of Ln complexes with Pyrroloquinoline quinone (PQQ), the cofactor of recently discovered lanthanide (Ln) dependent methanol dehydrogenase (MDH) enzymes. In this context, we have been able to elucidate the molecular structure of a bio-relevant europium PQQ complex for the first time outside a protein environment. Read More

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http://dx.doi.org/10.1002/chem.202002653DOI Listing

From Cultural Competence to Cultural Respect: A Critical Review of Six Models.

J Nurs Educ 2020 Jun;59(6):311-318

Background: Despite the development of cultural competence models in response to the increase in cultural diversity in the United States, health disparities based on ethnicity and cross-cultural mismatches in health care practices still exist.

Method: This article critically reviews six noteworthy conceptual models of cultural competence and enlists multilayered definitions of culture from cultural anthropology, critical multicultural education, and critical literary theory, as well as critical discourse analytical tools to deconstruct these frameworks.

Results: Although these models assist providers to become more culturally sensitive, they can essentialize and oversimplify patients' cultural experience, as well as mask the dynamism and complexities of their communities and power relations. Read More

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http://dx.doi.org/10.3928/01484834-20200520-03DOI Listing

Redesign and validation of a computer programming course using Inductive Teaching Method.

PLoS One 2020 4;15(6):e0233716. Epub 2020 Jun 4.

Department of Computer Science, COMSATS University Islamabad, Abbottabad, Pakistan.

Inductive Teaching Method (ITM) promotes effective learning in technological education (Felder & Silverman, 1988). Students prefer ITM more as it makes the subject easily understandable (Goltermann, 2011). The ITM motivates the students to actively participate in class activities and therefore could be considered a better approach to teach computer programming. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233716PLOS

Reduction of non-typeable results using a plasmid oriented Lymfogranuloma venereum PCR for typing of Chlamydia trachomatis positive samples.

PLoS One 2020 4;15(6):e0233990. Epub 2020 Jun 4.

Public Health Laboratory (GGD) Amsterdam, Department of infectious diseases, Amsterdam, The Netherlands.

Objectives: Typing of Chlamydia trachomatis (CT) is traditionally performed by characterising the ompA gene, resulting in more than a dozen different genovars, A to L. Type L is associated with Lymphogranuloma venereum (LGV) and commonly screened for using PCR, targeting the chromosomal pmpH gene. We aimed to develop and validate a new CT/LGV plasmid-based typing assay targeting the pgp3 gene, to increase sensitivity and thus reduce the number of non-typeable results. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233990PLOS

Assessment of transdermal delivery of topical compounds in skin scarring using a novel combined approach of Raman spectroscopy and HPLC.

Adv Wound Care (New Rochelle) 2020 Jun 4. Epub 2020 Jun 4.

University of Manchester, Plastic & Reconstructive Surgery Research, Manchester Institute of Biotechnology, Manchester, United Kingdom of Great Britain and Northern Ireland.

Objective: The goal of any topical formulation is efficient transdermal delivery of its active components. However, delivery of compounds can be problematic with penetration through tough layers of fibrotic dermal scar tissue.

Approach: We propose a new combined approach using high performance liquid chromatography (HPLC) and Raman spectroscopy (RS) in assessment of penetration of topicals used in scar management. Read More

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http://dx.doi.org/10.1089/wound.2020.1154DOI Listing

All-Mode Quantum-Classical Path Integral Simulation of Bacteriochlorophyll Dimer Exciton-Vibration Dynamics.

J Phys Chem B 2020 Jun 4. Epub 2020 Jun 4.

Department of Chemistry, University of Illinois, Urbana, Illinois 61801, United States.

We use the quantum-classical path integral (QCPI) methodology to report numerically exact, fully quantum mechanical results for the exciton-vibration dynamics in the bacteriochlorophyll dimer, including all 50 coupled vibrational normal modes of each bacteriochlorophyll explicitly with parameters obtained from spectroscopic Huang-Rhys factors. We present a coordinate transformation that maps the dimer on a spin-Boson Hamiltonian with a single collective bath. We consider two vibrational initial conditions which correspond to a Franck-Condon excitation or to modes initially equilibrated with the excited monomer. Read More

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http://dx.doi.org/10.1021/acs.jpcb.0c03032DOI Listing

Human intestinal microbiota derived metabolism signature from a North American native botanical Oplopanax horridus with UPLC/Q-TOF-MS analysis.

Biomed Chromatogr 2020 Jun 4:e4911. Epub 2020 Jun 4.

Tang Center for Herbal Medicine Research, and Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA.

Oplopanax horridus, distributed in North America, is an herbal medicine traditionally used by Pacific indigenous peoples for various medical conditions. After oral ingestion, constituents in O. horridus extract (OhE) could be converted to their metabolites by enteric microbiome before absorption. Read More

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http://dx.doi.org/10.1002/bmc.4911DOI Listing

Application of chemometric modelling to UV-Vis spectroscopy: development of simultaneous API and critical excipient assay in a liquid solution continuous flow.

Pharm Dev Technol 2020 Jun 4:1-11. Epub 2020 Jun 4.

Product Supply Innovation, Global Technical Operations Manufacturing Technology & Science, Ferring International Center SA, St-Prex, Switzerland.

By applying Chemometric modelling to UV-Vis spectroscopy, a single Multi-Variate Data Analysis predictive model, for in-line monitoring of two component concentrations in solution formulation, was developed. The purpose of this article is to summarize the experimental development work performed for the UV-Vis analytical method and calibration modelling to determine the content of active pharmaceutical ingredient Carbetocin and the content of one critical excipient (l-methionine) in PABAL® solution for injection 100 µg/mL. Read More

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http://dx.doi.org/10.1080/10837450.2020.1770789DOI Listing

Multivariate Pattern Analysis Techniques for Electroencephalography Data to Study Flanker Interference Effects.

Int J Neural Syst 2020 Jun 4:2050024. Epub 2020 Jun 4.

Mind, Brain and Behavior Research Center, Department of Experimental Psychology, University of Granada, Granada, 18071 Spain.

A central challenge in cognitive neuroscience is to understand the neural mechanisms that underlie the capacity to control our behavior according to internal goals. Flanker tasks, which require responding to stimuli surrounded by distracters that trigger incompatible action tendencies, are frequently used to measure this conflict. Even though the interference generated in these situations has been broadly studied, multivariate analysis techniques can shed new light into the underlying neural mechanisms. Read More

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http://dx.doi.org/10.1142/S0129065720500240DOI Listing

Iron(II) Spin Crossover Complexes Based on a Redox Active Equatorial Schiff-Base-Like Ligand.

Inorg Chem 2020 Jun 4. Epub 2020 Jun 4.

Inorganic Chemistry IV, University of Bayreuth, Universitätsstraße 30, 95448 Bayreuth, Germany.

In this work, two iron(II) coordination compounds with a NO coordinating Schiff base-like ligand bearing a redox active tetrathiafulvalene (TTF) unit and pyridine or -1,2-bis(4-pyridylethylene) as an axial ligand are synthesized. Crystals suitable for single X-ray structure analysis were obtained for the new ligand. The complexes were characterized by magnetic susceptibility measurements, -dependent UV-vis spectroscopy, and cyclic voltammetry. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.0c00725DOI Listing

Schrӧdinger Cat State Spectroscopy- A New Frontier for Analytical Chemistry.

Authors:
John C Wright

Anal Chem 2020 Jun 4. Epub 2020 Jun 4.

The invention of the laser generated great excitement because its ability to create quantum state coherences could form a new family of coherent spectroscopies that were the optical analogue of multidimensional nuclear magnetic resonance (NMR). The full realization of this promise has not yet been realized but the pathway forward is clear. The path involves the use of multiple, tunable lasers that create a Schrӧdinger cat state where the system is simultaneously in a mixture of vibrational and/or electronic states. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01662DOI Listing

Elution from intact and broken vaginal contraceptive rings: an in vitro study.

Eur Rev Med Pharmacol Sci 2020 May;24(10):5668-5675

Exeltis Healthcare SL, Ismaning, Germany.

Objective: The in vitro elution of the active substances etonogestrel (ETO) and ethinylestradiol (EE) of Ornibel® (a vaginal delivery system) was determined after a deliberate breakage of the vaginal contraceptive ring and compared to the standard elution and hormone release of intact rings under the same experimental conditions.

Materials And Methods: Ornibel® intact and broken vaginal rings were placed in a dissolution buffer and subject to a repetitive sampling of ETO and EE following a standardized in vitro elution (IVE) procedure for 21 days. The hormone dissolution profile was determined by HPLC using a fully validated analytical method. Read More

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http://dx.doi.org/10.26355/eurrev_202005_21358DOI Listing

Novel SERS-based process analysis for label-free segmented flow screenings.

Lab Chip 2020 Jun 4. Epub 2020 Jun 4.

Fraunhofer Institute for Chemical Technology, Pfinztal 76327, Germany.

In microfluidic segmented flow processes label-free analytical techniques like surface enhanced Raman spectroscopy (SERS) can reveal the chemical composition of the individual droplet contents. The SERS system developed in this work enables a simple connection to micro segmented flow processes through miniaturization. The concept is based on the parallelization of silver/polyacrylamide composite SERS spots on a carrier plate on which the segments are deposited. Read More

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http://dx.doi.org/10.1039/d0lc00367kDOI Listing

Expression of classical HLA class I antigens, HLA-E, and HLA-G is adversely prognostic in pancreatic cancer patients.

Cancer Sci 2020 Jun 3. Epub 2020 Jun 3.

Division of Biomedical Sciences, St. Luke's International University Graduate School of Public Health, Tokyo, Japan.

The expression of classical HLA class I antigens (HLA-I) on the surfaces of cancer cells allows cytotoxic T cells to recognize and eliminate these cells. Reduction or loss of HLA-I is a mechanism of escape from antitumor immunity. The present study aimed to investigate the clinicopathological impacts of HLA-I and non-classical HLA-I antigens expressed on pancreatic ductal adenocarcinoma (PDAC) cells. Read More

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http://dx.doi.org/10.1111/cas.14514DOI Listing

Infrared Permittivity of the Biaxial van der Waals Semiconductor α-MoO from Near- and Far-Field Correlative Studies.

Adv Mater 2020 Jun 3:e1908176. Epub 2020 Jun 3.

Department of Physics, University of Oviedo, Oviedo, 33006, Spain.

The biaxial van der Waals semiconductor α-phase molybdenum trioxide (α-MoO ) has recently received significant attention due to its ability to support highly anisotropic phonon polaritons (PhPs)-infrared (IR) light coupled to lattice vibrations-offering an unprecedented platform for controlling the flow of energy at the nanoscale. However, to fully exploit the extraordinary IR response of this material, an accurate dielectric function is required. Here, the accurate IR dielectric function of α-MoO is reported by modeling far-field polarized IR reflectance spectra acquired on a single thick flake of this material. Read More

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http://dx.doi.org/10.1002/adma.201908176DOI Listing

Evidence for the Cost-Effectiveness of Return-to-Work Interventions for Mental Illness Related Sickness Absences: A Systematic Literature Review.

J Occup Rehabil 2020 Jun 3. Epub 2020 Jun 3.

Library Services, Centre for Addiction and Mental Health, 33 Russell Street, Toronto, ON, M5S 2S1, Canada.

Purpose The purpose of this systematic literature review (SLR) is to examine the state of knowledge about the cost-effectiveness of return-to-work (RTW) interventions targeted at workers with medically certified sickness absences related to mental disorders. Our SLR addresses the question, "What is the evidence for the cost-effectiveness of RTW interventions for mental illness related sickness absences?" Methods This SLR used a reviewer pair multi-phase screening of publically available peer-reviewed studies published between 2002 and 2019. Five electronic databases were searched: (1) MEDLINE 1946-Present, (2) MEDLINE: Epub-Ahead of Print and In-Process, (3) PsycINFO, (4) Econlit, and (5) Web of Science. Read More

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http://dx.doi.org/10.1007/s10926-020-09904-zDOI Listing

Lateral flow immunoassay for 5-hydroxyflunixin based on near-infrared fluorescence molecule as an alternative label to gold nanoparticles.

Mikrochim Acta 2020 Jun 3;187(6):368. Epub 2020 Jun 3.

State Key Laboratory of Animal Nutrition, and Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China.

A high-affinity monoclonal antibody (mAb) has been prepared and separately a gold nanoparticle (AuNP)-based and a near-infrared (NIR) fluorescence-based lateral flow immunoassay (LFA) developed for determination of 5-hydroxyflunixin residue in raw milk. The AuNP and IRDye® 800CW were used to label anti-5-hydroxyflunixin mAb to form the AuNP-mAb and NIR dye-mAb conjugates, respectively. Quantitative determination of 5-hydroxyflunixin was achieved by imaging the optical or fluorescence intensity of the AuNP-mAb and NIR dye-mAb captured on the test line. Read More

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http://dx.doi.org/10.1007/s00604-020-04338-zDOI Listing

New findings on urinary prostate cancer metabolome through combined GC-MS and H NMR analytical platforms.

Metabolomics 2020 Jun 3;16(6):70. Epub 2020 Jun 3.

UCIBIO/REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.

Introduction: The inherent sensitivity of metabolomics allows the detection of subtle alterations in biological pathways, making it a powerful tool to study biomarkers and the mechanisms that underlie cancer.

Objectives: The purpose of this work was to characterize the urinary metabolic profile of prostate cancer (PCa) patients and cancer-free controls to obtain a holistic coverage of PCa metabolome.

Methods: Two groups of samples, a training set (n = 41 PCa and n = 42 controls) and an external validation set (n = 18 PCa and n = 18 controls) were analyzed using a dual analytical platform, namely gas chromatography-mass spectrometry (GC-MS) and proton nuclear magnetic resonance spectroscopy (H NMR). Read More

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http://dx.doi.org/10.1007/s11306-020-01691-1DOI Listing

A Fluorescent Chemosensor Based on Schiff Base for the Determination of Zn, Cdand Hg.

Authors:
E K İnal

J Fluoresc 2020 Jun 4. Epub 2020 Jun 4.

Department of Chemistry, Faculty of Science, Ankara University, Ankara, 06100, Turkey.

Metal complexes were obtained by the reaction of zinc, cadmium and mercury(II) salts with Schiff base HL (N(salicylidene)benzylamine). HL was synthesized by the condensation reaction of benzylamine and 2-hydroxybenzaldehyde. The fluorescence properties of the Schiff base and its metal complexes were studied in ethanol-water solutions. Read More

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http://dx.doi.org/10.1007/s10895-020-02563-6DOI Listing

Magnetic bead-enzyme assemble for triple-parameter telomerase detection at single-cell level.

Anal Bioanal Chem 2020 Jun 3. Epub 2020 Jun 3.

Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen, 518039, Guangdong, China.

In this work, we developed a triple-parameter strategy for the detection of telomerase activity from cancer cells and urine samples. This strategy was developed based on magnetic bead-enzyme hybrids combined with fluorescence analysis, colorimetric assay, or adenosine triphosphate (ATP) meter as readout. The application of magnetic bead-enzyme hybrids has the advantages of magnetic separation and signal amplification. Read More

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http://dx.doi.org/10.1007/s00216-020-02741-2DOI Listing

Expanded coverage of non-targeted LC-HRMS using atmospheric pressure chemical ionization: a case study with ENTACT mixtures.

Anal Bioanal Chem 2020 Jun 3. Epub 2020 Jun 3.

U.S. Environmental Protection Agency, Office of Research & Development, National Exposure Research Laboratory, 109 TW Alexander Dr., Research Triangle Park, NC, 27711, USA.

Non-targeted analysis (NTA) is a rapidly evolving analytical technique with numerous opportunities to improve and expand instrumental and data analysis methods. In this work, NTA was performed on eight synthetic mixtures containing 1264 unique chemical substances from the U.S. Read More

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http://dx.doi.org/10.1007/s00216-020-02716-3DOI Listing

Ultrasound-assisted dispersive solid-phase microextraction of capecitabine by multi-stimuli responsive molecularly imprinted polymer modified with chitosan nanoparticles followed by HPLC analysis.

Mikrochim Acta 2020 Jun 3;187(6):366. Epub 2020 Jun 3.

Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.

An ultrasonic-assisted dispersive solid-phase microextraction was developed by a multi-stimuli responsive molecularly imprinted polymer based on chain transfer agent-modified chitosan nanoparticles for enrichment and separation of trace capecitabine in a real sample. The synthesized particles were carefully characterized and it was found that a uniform pH-sensitive imprinted polymeric shell is obtained on the surface of FeO@chitosan core with enough saturation magnetization (29 emu g). Desirable adsorption capacity (91 mg g) and high imprinting factor (IF = 3. Read More

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http://dx.doi.org/10.1007/s00604-020-04345-0DOI Listing

Multibody Analysis and Control of a Full-Wrist Exoskeleton for Tremor Alleviation.

J Biomech Eng 2020 Jun 4. Epub 2020 Jun 4.

Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia 24061, USA.

Uncontrollable shaking in the human wrist, caused by pathological tremor, can significantly undermine the power and accuracy in object manipulation. In this paper, the design of a Tremor Alleviating Wrist Exoskeleton (TAWE) is introduced. Unlike the works in the literature that only consider the flexion/extension motion, in this paper, we model the wrist joint as a constrained 3D rotational joint accounting for the coupled flexion/extension (FE) and radial/ulnar deviation (RUD) motions. Read More

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http://dx.doi.org/10.1115/1.4047424DOI Listing

Phenyl doped graphitic carbon nitride nanosheets for sensing of copper ions in living cells.

Analyst 2020 Jun 4. Epub 2020 Jun 4.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.

Copper (Cu) is a vital metal element for humans and animals. Monitoring and evaluating the concentration level of Cu2+ in a biological body is an effective way to prevent a variety of diseases. In this work, phenyl doped graphitic carbon nitride (PDCN) nanosheets with strong green fluorescence exhibited a sensitive and selective detection for Cu2+ with a linear range from 0. Read More

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http://dx.doi.org/10.1039/d0an00795aDOI Listing

Interfacing gene circuits with microelectronics through engineered population dynamics.

Sci Adv 2020 May 22;6(21):eaaz8344. Epub 2020 May 22.

BioCircuits Institute, University of California, San Diego, La Jolla, CA, USA.

While there has been impressive progress connecting bacterial behavior with electrodes, an attractive observation to facilitate advances in synthetic biology is that the growth of a bacterial colony can be determined from impedance changes over time. Here, we interface synthetic biology with microelectronics through engineered population dynamics that regulate the accumulation of charged metabolites. We demonstrate electrical detection of the bacterial response to heavy metals via a population control circuit. Read More

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http://dx.doi.org/10.1126/sciadv.aaz8344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244307PMC

Exosome-templated nanoplasmonics for multiparametric molecular profiling.

Sci Adv 2020 May 6;6(19):eaba2556. Epub 2020 May 6.

Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore.

Exosomes are nanoscale vesicles distinguished by characteristic biophysical and biomolecular features; current analytical approaches, however, remain univariate. Here, we develop a dedicated platform for multiparametric exosome analysis-through simultaneous biophysical and biomolecular evaluation of the same vesicles-directly in clinical biofluids. Termed templated plasmonics for exosomes, the technology leverages in situ growth of gold nanoshells on vesicles to achieve multiselectivity. Read More

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http://dx.doi.org/10.1126/sciadv.aba2556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202874PMC

Quantifying methane emissions from the largest oil-producing basin in the United States from space.

Sci Adv 2020 Apr 22;6(17):eaaz5120. Epub 2020 Apr 22.

School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Using new satellite observations and atmospheric inverse modeling, we report methane emissions from the Permian Basin, which is among the world's most prolific oil-producing regions and accounts for >30% of total U.S. oil production. Read More

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http://dx.doi.org/10.1126/sciadv.aaz5120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176423PMC

MEASUREMENT OF THE HYDRAULIC CONDUCTIVITY OF THE VITREOUS HUMOR.

J Porous Media 2020 ;23(2):195-206

Department of Aerospace & Mechanical Engineering, University of Southern California, USC Viterbi School of Engineering, Los Angeles, CA 90089-1453.

The hydraulic conductivity of the vitreous humor has been measured for the bovine eye. The experiment was carried out by placing it within upright cylindrical chamber, open at both ends, and letting its liquid content drain out of the bottom opening by gravity, through a 20m nylon mesh filter. Additional negative pressure was provided at the exit by a hanging drainage tube. Read More

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http://dx.doi.org/10.1615/JPorMedia.2020028229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269170PMC
January 2020

Generalized Maxwell projections for multi-mode network Photonics.

Sci Rep 2020 Jun 3;10(1):9038. Epub 2020 Jun 3.

PRIMALIGHT, Faculty of Electrical Engineering, Applied Mathematics and Computational Sci-4ence, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.

The design of optical resonant systems for controlling light at the nanoscale is an exciting field of research in nanophotonics. While describing the dynamics of few resonances is a relatively well understood problem, controlling the behavior of systems with many overlapping states is considerably more difficult. In this work, we use the theory of generalized operators to formulate an exact form of spatio-temporal coupled mode theory, which retains the simplicity of traditional coupled mode theory developed for optical waveguides. Read More

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http://dx.doi.org/10.1038/s41598-020-65293-6DOI Listing

Optimization and evaluation of a multiplex quantitative PCR assay for detection of nucleic acids in human blood from patients with spotted fever rickettsiosis, typhus rickettsiosis, scrub typhus, monocytic ehrlichiosis, and granulocytic anaplasmosis.

J Clin Microbiol 2020 Jun 3. Epub 2020 Jun 3.

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Spotted fever group (SFGR) and typhus group (TGR) rickettsioses, scrub typhus (caused by , [OT]), ehrlichiosis, and anaplasmosis often present as undifferentiated fever but are not treated by agents (penicillins and cephalosporins) typically used for acute febrile illness. Inability to diagnose these infections when the patient is acutely ill leads to excess morbidity and mortality. Failure to confirm these infections retrospectively if a convalescent blood sample is not obtained also impairs epidemiologic and clinical research. Read More

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http://dx.doi.org/10.1128/JCM.01802-19DOI Listing

A mouse SWATH-MS reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.

Dis Model Mech 2020 Jun 3. Epub 2020 Jun 3.

Division of Molecular Pathology, The Institute of Cancer Research, London, UK

SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. Read More

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http://dx.doi.org/10.1242/dmm.044586DOI Listing

Nanofibers as advanced sorbents for on-line solid phase extraction in liquid chromatography: A tutorial.

Anal Chim Acta 2020 Jul 22;1121:83-96. Epub 2020 Apr 22.

Charles University, Faculty of Pharmacy, The Department of Analytical Chemistry, Ak. Heyrovského 1203, 500 05, Hradec Králové, Czech Republic. Electronic address:

Polymers in nanofiber format promise a great potential as sorbents for extraction techniques. This tutorial provides an overview of direct coupling of extraction techniques based on nanofibers to liquid chromatography. Arrangements of the fibers in conventional extraction cartridges are demonstrated. Read More

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http://dx.doi.org/10.1016/j.aca.2020.04.045DOI Listing

Enhanced performance of an electrochemical aptasensor for real-time detection of vascular endothelial growth factor (VEGF) by nanofabrication and ratiometric measurement.

Anal Chim Acta 2020 Jul 6;1121:74-82. Epub 2020 May 6.

International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China; Graduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, 2052, Australia; Australian Centre for NanoMedicine and UNSW Digital Grid Futures Institute, University of New South Wales, Sydney, 2052, Australia. Electronic address:

Achieving a biosensing interface without baseline drift caused by variables in matrix samples is essential for real-time detection of analytes. In this study, we developed a molecular beacon based electrochemical aptasensor to realize the ratiometric signal quantification of VEGF in serum by surface modification of nanocomposites of graphene oxide/methylene blue (GO/MB) and AuNPs followed by the attachment of ferrocene-labeled aptamer (aptamer-Fc) against VEGF. The presence of VEGF can trigger the configuration change of aptamer-Fc, resulting in the redox probe Fc being far away from the electrode surface to attenuate the electrochemical communication between electrode and Fc. Read More

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http://dx.doi.org/10.1016/j.aca.2020.05.003DOI Listing

On-line microplasma decomposition of gaseous phase interference for solid sampling mercury analysis in aquatic food samples.

Anal Chim Acta 2020 Jul 27;1121:42-49. Epub 2020 Apr 27.

Institute of Quality Standard and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, And Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing, 100081, China.

In this work, dielectric barrier discharge (DBD) was first utilized to eliminate gaseous phase interference from complicated solid sample. So, a novel solid sampling Hg analyzer was first designed using a coaxial DBD reactor to replace catalytic pyrolysis furnace for sensitive mercury determination in aquatic food samples. The Hg analyzer mainly comprised an electrothermal vaporizer (ETV), a DBD reactor to decompose gaseous interfering substances including volatile organic compounds (VOCs), a gold-coil Hg trap to eliminate matrix interference and an atomic fluorescence spectrometer (AFS) as detector. Read More

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http://dx.doi.org/10.1016/j.aca.2020.04.057DOI Listing

Calcium-cation-doped polydopamine-modified 2D black phosphorus nanosheets as a robust platform for sensitive and specific biomolecule sensing.

Anal Chim Acta 2020 Jul 30;1121:1-10. Epub 2020 Apr 30.

Department of Pharmacy, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041, China. Electronic address:

Many polymer decorated/modified 2D nanomaterials have been developed as enhanced drug delivery systems and photothermal theranostic nanoagents. However, few reports describe the use of these novel nanomaterials as nanoplatforms for biomolecule sensing. Herein, we used calcium-cation-doped polydopamine-modified (PDA-modified) 2D black phosphorus (BP) nanosheets (BP@PDA) as a sensing nanoplatform for the detection of nucleic acids and proteins in complex biological samples. Read More

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http://dx.doi.org/10.1016/j.aca.2020.04.072DOI Listing

Cerebrospinal fluid penetration of targeted therapeutics in pediatric brain tumor patients.

Acta Neuropathol Commun 2020 Jun 3;8(1):78. Epub 2020 Jun 3.

Department of Pediatrics and Adolescent Medicine and Comprehensive Center for Pediatrics, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.

Treatment with small-molecule inhibitors, guided by precision medicine has improved patient outcomes in multiple cancer types. However, these compounds are often not effective against central nervous system (CNS) tumors. The failure of precision medicine approaches for CNS tumors is frequently attributed to the inability of these compounds to cross the blood-brain barrier (BBB), which impedes intratumoral target engagement. Read More

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http://dx.doi.org/10.1186/s40478-020-00953-2DOI Listing

A tissue level atlas of the healthy human virome.

BMC Biol 2020 Jun 4;18(1):55. Epub 2020 Jun 4.

Division of Systems Virology, Department of Infectious Disease Control, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 1088639, Japan.

Background: Human-resident microbes can influence both health and disease. Investigating the microbiome using next-generation sequencing technology has revealed examples of mutualism and conflict between microbes and humans. Comparing to bacteria, the viral component of the microbiome (i. Read More

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http://dx.doi.org/10.1186/s12915-020-00785-5DOI Listing