234,036 results match your criteria Analytical sciences : the international journal of the Japan Society for Analytical Chemistry[Journal]


Development and Validation of Molecular Assays for Limited Tissue Samples.

Acta Cytol 2019 Apr 17:1-8. Epub 2019 Apr 17.

Center for Personalized Diagnostics, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA,

As the value of molecular testing of cancer specimens increases, the number of tests imposed on tumor specimens also increases, often in tension with the amount of tumor material available. To develop and validate molecular assays for limited specimens, there are specific concerns that must be addressed, including DNA quality, quantity, and abundance; the number of targets/ability to multiplex; and the analytical sensitivity and specificity of the assay itself. Ultimately, weighing these considerations during assay validation in the overall context of clinical utility and laboratory workflow is critical for delivering the highest level of personalized care to patients. Read More

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https://www.karger.com/Article/FullText/499109
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http://dx.doi.org/10.1159/000499109DOI Listing
April 2019
1 Read

Levels, spatial distribution and isomer profiles of perfluoroalkyl acids in soil, groundwater and tap water around a manufactory in China.

Chemosphere 2019 Apr 9;227:305-314. Epub 2019 Apr 9.

Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.

In this study, 32 surface soil samples, 24 groundwater samples, and 6 tap water samples were collected around a perfluorosulfonates (PFSAs) manufactory in China to analyze the distributions of perfluoroalkyl acids (PFAAs) including linear and branched isomers. The total concentrations of PFAAs (∑PFAAs) ranged from 1.30 to 913 ng/g on a dry weight basis (dw), 31. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.027DOI Listing

Quantitation of vitamin K1 in serum using online SPE-LC-MS/MS and the challenges of working with vitamin K.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 7;1117:41-48. Epub 2019 Apr 7.

Department of Clinical Biochemistry and Immunology, Lillebaelt Hospital, Vejle, Denmark.

Vitamin K1 (phylloquinone) is one of the vitamin Ks. Several studies have previously investigated the role of vitamin K1 status in respect to disease, but without consistent results. Since vitamin K deficiency has been associated with different disease states it is important to develop a biochemical analysis with sufficient sensitivity and a low limit of quantitation (LOQ). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183176
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http://dx.doi.org/10.1016/j.jchromb.2019.04.010DOI Listing
April 2019
1 Read

Perylenequinone-based "turn on" fluorescent probe for hydrogen sulfide with high sensitivity in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Mar 29;218:206-212. Epub 2019 Mar 29.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:

Hydrogen sulfide (HS) is a kind of gaseous signal molecule in many physiological processes. In order to detect HS, a novel "turn on" fluorescent probe 6,12-dihydroxyperylene-1,7-dione (DPD) was designed and synthesized. The probe DPD is fluorescence silence, while the addition of HS induces an obvious green fluorescence with an obvious color change from dark blue to yellow-green. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13861425193036
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http://dx.doi.org/10.1016/j.saa.2019.03.112DOI Listing
March 2019
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Patterns of funerary variability, diet, and developmental stress in a Celtic population from NE Italy (3rd-1st c BC).

PLoS One 2019 17;14(4):e0214372. Epub 2019 Apr 17.

Department of Anthropology and Anthropological Museum, Universität Zürich-Irchel, Zürich, Switzerland.

Little is known about the types of social organization characterizing the pre-Roman Celtic populations of Italy. Here, we explore the funerary variability characterizing the late Iron Age site of Seminario Vescovile (SV: Verona, Italy, 3rd-1st c. BC), and test its possible correlation to diet and relative exposure to developmental stressors. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214372PLOS

Transferability of local density assisted implicit solvation models for homogeneous fluid mixtures.

J Chem Theory Comput 2019 Apr 17. Epub 2019 Apr 17.

The application of bottom-up coarse grained (CG) models to study the equilibrium mixing behavior of liquids is rather challenging, since these models can be significantly influenced by the density or the concentration of the state chosen during parametrization. This dependency leads to low transferability in density/concentration space and has been one of the major limitations in bottom-up coarse graining. Recent approaches proposed to tackle this shortcoming range from the addition of thermodynamic constraints, to an extended ensemble parametrization, to the addition of supplementary terms to the system's Hamiltonian. Read More

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http://dx.doi.org/10.1021/acs.jctc.8b01170DOI Listing

Optical multiplexed bioassays improve biomedical diagnostics.

Angew Chem Int Ed Engl 2019 Apr 17. Epub 2019 Apr 17.

CHINA.

Conventional analytical methods based on single detection to determine one disease marker are usually not sufficient and accurate because the progression of disease generally involves multiple chemicals and biomolecules. The drive for simultaneous analysis of multiple targets, which plays a key role in both basic biomedical research and clinical applications, demands the development of multiplexed bioassays with high-throughput. In this minireview, we systematically summarize the recent progress of optical multiplexed analytical techniques to improve biomedical diagnostics, in which fluorescence and surface enhanced Raman scattering (SERS) with distinctive optical features are chosen as the main readout signals. Read More

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http://dx.doi.org/10.1002/anie.201901964DOI Listing

Evolution of biosequence search algorithms: a brief survey.

Authors:
Gregory Kucherov

Bioinformatics 2019 Apr 17. Epub 2019 Apr 17.

CNRS and LIGM/University of Paris-Est, Marne-la-Vallée, France.

Motivation: Although modern high-throughput biomolecular technologies produce various types of data, biosequence data remains at the core of bioinformatic analyses. However, computational techniques for dealing with this data evolved dramatically.

Results: In this bird's-eye review, we overview the evolution of main algorithmic techniques for comparing and searching biological sequences. Read More

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http://dx.doi.org/10.1093/bioinformatics/btz272DOI Listing

Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array Detection.

J Chromatogr Sci 2019 Apr 17. Epub 2019 Apr 17.

Pharmaceutical Analysis Department, Faculty of Pharmacy, Damanhour University, 22511, Damanhour, Egypt.

Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography-diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma. Read More

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http://dx.doi.org/10.1093/chromsci/bmz031DOI Listing

Simultaneous Quantification of Chlorpheniramine, Phenylephrine, Guaifenesin in Presence of Preservatives with Detection of Related Substance Guaiacol in their Cough Syrup by RP-HPLC and TLC-Densitometric Methods.

J Chromatogr Sci 2019 Apr 17. Epub 2019 Apr 17.

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo 11562, Egypt.

Two sensitive chromatographic methods have been developed, and validated for chlorpheniramine maleate (CM), phenylephrine (PE) and guaifenesin (GF) determination in their mixture and in presence of GF related substance guaiacol (GL) and preservative namely; sodium benzoate (NaB). The first method was based on thin layer chromatographic separation (TLC) followed by densitometric determination of the separated spots. The separation was achieved using silica gel 60 F254 TLC plates and ethyl acetate: methanol: toluene: ammonia (7:1. Read More

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http://dx.doi.org/10.1093/chromsci/bmz027DOI Listing

Sample design and participation in the Educatel Study.

Cad Saude Publica 2019 Apr 15;35Suppl 1(Suppl 1):e00167217. Epub 2019 Apr 15.

Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.

Educatel Brazil 2015/2016 was a cross-sectional study conducted by telephone interview with the aim of producing information on health and absenteeism in Brazilian schoolteachers. The nationally representative sampling plan was based on the simple stratified sampling method, with stratification defined to meet the analytical domains established for the study (five major geographic regions, two census areas, four age brackets, sex, three types of school administration, five types of teacher employment, and six grade levels) and selection by simple random sampling of teachers in each stratum. Teacher selection was based on the 2014 School Census conducted by the Brazilian National Institute for Educational Studies and Research "Anísio Teixeira". Read More

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S
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http://dx.doi.org/10.1590/0102-311X00167217DOI Listing
April 2019
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Polydopamine nanosphere@silver nanoclusters for fluorescence detection of multiplex tumor markers.

Nanoscale 2019 Apr 17. Epub 2019 Apr 17.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China. and University of Science and Technology of China, Hefei 230026, P. R. China and Jinan Guokeyigong Science and Technology Development Co., Ltd, Jinan 250103, P. R. China.

There is an increasing demand to establish a convenient and stable analytical methodology for screening multiplex tumor markers in early diagnosis of cancers. In this work, an innovative fluorescence method is proposed for simultaneous detection of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA). A polydopamine nanosphere@silver nanocluster (PDAN@AgNC) system is introduced for fluorescence quenching and recovery. Read More

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http://xlink.rsc.org/?DOI=C9NR01307E
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http://dx.doi.org/10.1039/c9nr01307eDOI Listing
April 2019
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Ca-, Sr-, and Ba-Coordination polymers based on anthranilic acid via mechanochemistry.

Dalton Trans 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, D-12489 Berlin, Germany.

Ca-, Sr-, and Ba-Based coordination polymers (CPs) were prepared mechanochemically by milling metal-hydroxide samples with anthranilic acid (oABAH). {[Ca(oABA)2(H2O)3]}n (1) consists of one-dimensional polymeric chains that are further connected by a hydrogen-bonding network. {[Sr(oABA)2(H2O)2]·H2O}n (2) is a one-dimensional CP in which water molecules bridge Sr2+ ions and increase the dimensionality by building an extended network. Read More

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http://dx.doi.org/10.1039/c9dt00991dDOI Listing

Equatorial ligand plane perturbations lead to a spin-state change in an iron(iii) porphyrin dimer.

Dalton Trans 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur-208016, India.

A complete reversal of the spin state of iron(iii) is observed upon a small change to the diporphyrin bridge from ethane to ethene by keeping all other factors intact. Combined analysis using single crystal X-ray structure determination, Mössbauer, variable-temperature magnetic, 1H NMR and EPR studies has confirmed the spin states of iron(iii) complexes both in solid and solution phases. Read More

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http://dx.doi.org/10.1039/c9dt01182jDOI Listing

Analytical performance of μ-groove silicon attenuated total reflection waveguides.

Analyst 2019 Apr 17. Epub 2019 Apr 17.

Insitute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

The analytical performance of micromachined μ-groove silicon attenuated total reflection (ATR) elements has been evaluated in a comparison of Fourier-transform infrared (FTIR) and quantum cascade laser (QCL) spectroscopy operating at mid-infrared (MIR) wavelengths. μ-Groove silicon ATR elements are highly efficient micromachined waveguides fabricated at a wafer scale at such low cost that they may be considered a consumable for single-time-use, e.g. Read More

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http://dx.doi.org/10.1039/c9an00417cDOI Listing

Characterization of natural polymers as functional barriers for cellulose-based packaging materials.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2019 Apr 17:1-13. Epub 2019 Apr 17.

a Institute of Analytical Chemistry and Food Chemistry , Graz University of Technology , Graz , Austria.

Cellulose-based packaging materials are currently the most commonly used food packaging materials due to their light weight, stability and affordable price. However, the use of recycled paper and board adds to the risk that undesirable substances migrate into the packed goods, since contaminants are not completely removed during the recycling process and can accumulate in the final product. The only available fast and practical solution that can be used to reduce the migration of these substances is the application of functional barriers in the packaging. Read More

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http://dx.doi.org/10.1080/19440049.2019.1600747DOI Listing
April 2019
4 Reads

Erythrocytes Polyunsaturated Fatty Acids Mediate Relationship between Dietary Patterns and Depression.

Int J Vitam Nutr Res 2019 Apr 17:1-8. Epub 2019 Apr 17.

1 Tehran University of Medical Sciences, Tehran, Iran.

This study aimed to examine the association between depression and dietary patterns via the intermediary role of erythrocytes polyunsaturated fatty acids (PUFA). In this individually matched case-control study, the dietary patterns were extracted for 330 individuals using factor analysis. Furthermore, erythrocyte PUFAs including n-3 and n-6 were assessed using a GC-Mass spectrometry analytical method for 84 people. Read More

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http://dx.doi.org/10.1024/0300-9831/a000586DOI Listing

Untargeted and Targeted Fingerprinting of Extra Virgin Olive Oil Volatiles by Comprehensive Two-Dimensional Gas Chromatography with Mass Spectrometry: Challenges in Long-Term Studies.

J Agric Food Chem 2019 Apr 17. Epub 2019 Apr 17.

Comprehensive two-dimensional gas chromatography coupled with mass spectrometric detection (GC×GC-MS) offers an information-rich basis for effective chemical fingerprinting of food. However, GC×GC-MS yields 2D-peak patterns (i.e. Read More

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http://pubs.acs.org/doi/10.1021/acs.jafc.9b01661
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http://dx.doi.org/10.1021/acs.jafc.9b01661DOI Listing
April 2019
1 Read

Site Selective Amide Reduction of Cyclosporine A Enables Diverse Derivation of an Important Cyclic Peptide.

Org Lett 2019 Apr 17. Epub 2019 Apr 17.

Caudill Laboratories, Department of Chemistry , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599-3290 , United States.

Site selective amide reductions of the cyclic undecapeptide, cyclosporine A, have been developed using the combination of a heteroleptic borane catalyst and a silane reductant. Tertiary silane MeEtSiH provides two unique cyclosporine A derivatives, one of which can be readily diversified in subsequent reactions. The secondary silane EtSiH enables divergent reactivity that uses a free hydroxyl group to direct the reduction. Read More

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http://dx.doi.org/10.1021/acs.orglett.9b01245DOI Listing

Development and optimization of a supercritical fluid chromatography tandem mass spectrometry method for the high-throughput determination of nimodipine in beagle plasma.

J Sep Sci 2019 Apr 17. Epub 2019 Apr 17.

Wuya College of Innovation, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang, 110016, PR China.

A simple, sensitive and efficient supercritical fluid chromatography tandem mass spectrometry method was established for the determination of nimodipine in beagle plasma. One-step protein precipitation with acetone was used to extract the analytes from the plasma. Nitrendipine was used as the internal standard. Read More

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http://dx.doi.org/10.1002/jssc.201900134DOI Listing

Sequential measurement of δ N, δ C and δ S values in archaeological bone collagen at the Scottish Universities Environmental Research Centre (SUERC): a new analytical frontier.

Rapid Commun Mass Spectrom 2019 Apr 16. Epub 2019 Apr 16.

Scottish Universities Environmental Research Centre, Scottish Enterprise Technology Park, Rankine Avenue, East Kilbride, G75 0QF, UK.

Rationale: The use of multi-isotopic analysis (δ N, δ C and δ S values) of archaeological bone collagen to assist in the interpretation of diet, movement and mobility of prehistoric populations is gradually increasing, yet many researchers have traditionally avoided investigating sulphur due to its very low concentrations (<0.3%) in mammalian collagen. For this reason, and as a consequence of analytical detection limits, sulphur is usually measured separately from carbon and nitrogen, which leads to longer analytical times and higher costs. Read More

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http://dx.doi.org/10.1002/rcm.8462DOI Listing

Artificial Microbial Arenas: Materials for Observing and Manipulating Microbial Consortia.

Adv Mater 2019 Apr 16:e1900284. Epub 2019 Apr 16.

Microverse Cluster, Friedrich Schiller University Jena, Neugasse 23, 07743, Jena, Germany.

From the smallest ecological niche to global scale, communities of microbial life present a major factor in system regulation and stability. As long as laboratory studies remain restricted to single or few species assemblies, however, very little is known about the interaction patterns and exogenous factors controlling the dynamics of natural microbial communities. In combination with microfluidic technologies, progress in the manufacture of functional and stimuli-responsive materials makes artificial microbial arenas accessible. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.2019002
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http://dx.doi.org/10.1002/adma.201900284DOI Listing
April 2019
1 Read

Disparities in the management of cutaneous malignant melanoma. A population-based high-resolution study.

Eur J Cancer Care (Engl) 2019 Apr 16:e13043. Epub 2019 Apr 16.

Navarra Public Health Institute - IdiSNA, Pamplona, Spain.

Population-based cancer registry data from three Spanish areas were used to assess the patterns of care and adherence to guidelines for cutaneous malignant melanoma. We included 934 cases diagnosed in 2009-2013. Completeness of the pathology reports, imaging for detecting distant metastasis and the use of sentinel lymph node biopsy (SLNB) were analysed. Read More

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http://dx.doi.org/10.1111/ecc.13043DOI Listing

Determination of Gas-Phase Ion Structures of Locally Polar Homopolymers Through High-Resolution Ion Mobility Spectrometry-Mass Spectrometry.

J Am Soc Mass Spectrom 2019 Apr 16. Epub 2019 Apr 16.

Department of Mechanical Engineering, IUPUI, 723 W Michigan st, Indianapolis, IN, 46202, USA.

The strong synergy arising from coupling two orthogonal analytical techniques such as ion mobility and mass spectrometry can be used to separate complex mixtures and determine structural information of analytes in the gas phase. A tandem study is performed using two systems with different gases and pressures to ascertain gas-phase conformations of homopolymer ions. Aside from spherical and stretched configurations, intermediate configurations formed by a multiply charged globule and a "bead-on-a-string" appendix are confirmed for polyethylene-glycol (PEG), polycaprolactone (PCL), and polydimethylsiloxane (PDMS). Read More

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http://dx.doi.org/10.1007/s13361-019-02184-9DOI Listing

The Role of Mass Spectrometry in the Cannabis Industry.

J Am Soc Mass Spectrom 2019 Apr 16. Epub 2019 Apr 16.

Q2 Solutions, LLC, 19 Brown Rd., Ithaca, NY, 14850, USA.

The focus of this critical insight article is a brief overview of analytical challenges the cannabis industry faces and how analytical chemists have new opportunities to demonstrate the merits of employing mass spectrometry for the chemical analysis of cannabis and its products. The current range of cannabis products extends from recreational use to medicines, edibles, beverages, and beyond. The standards employed to assure product quality, integrity, and safety are lacking compared to those currently used by the pharmaceutical, food, and beverage industries. Read More

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http://dx.doi.org/10.1007/s13361-019-02164-zDOI Listing

Interrogating Proton Affinities of Organophosphonate Species Via Atmospheric Flow Tube Mass Spectrometry and Computational Methods.

J Am Soc Mass Spectrom 2019 Apr 15. Epub 2019 Apr 15.

Washington State University, Pullman, WA, 99164, USA.

Within trace vapor analysis in environmental monitoring, defense, and industry, atmospheric flow tube mass spectrometry (AFT-MS) can fill a role that incorporates non-contact vapor analysis with the selectivity and low detection limits of mass spectrometry. AFT-MS has been applied to quantitating certain explosives by selective clustering with nitrate and more recently applied to detecting tributyl phosphate and dimethyl methylphosphonate as protonated species. Developing AFT-MS methods for organophosphorus species is appealing, given that this class of compounds includes a range of pollutants, chemical warfare agent (CWA) simulants, and CWA degradation products. Read More

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http://dx.doi.org/10.1007/s13361-019-02202-wDOI Listing

Reduction of Dimensionality in Monte Carlo Simulation of Diffusion in Extracellular Space Surrounding Cubic Cells.

Neurochem Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Neuroscience and Physiology, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA.

The real-time iontophoretic method has measured volume fraction and tortuosity of the interstitial component of extracellular space in many regions and under different conditions. To interpret these data computer models of the interstitial space (ISS) of the brain are constructed by representing cells as Basic Cellular Structures (BCS) surrounded by a layer of ISS and replicating this combination to make a 3D ensemble that approximates brain tissue with a specified volume fraction. Tortuosity in such models is measured by releasing molecules of zero size into the ISS and allowing them to execute random walks in the ISS of the ensemble using a Monte Carlo algorithm. Read More

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http://dx.doi.org/10.1007/s11064-019-02793-6DOI Listing

Environmentally Benign Carbon Nanodots Prepared from Lemon for the Sensitive and Selective Fluorescence Detection of Fe(III) and Tannic Acid.

J Fluoresc 2019 Apr 16. Epub 2019 Apr 16.

Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.

Photoluminescent carbon nanodots (CNDs) were prepared using a biocarbon source of lemon extract. The obtained CNDs are of spherical shape and are enriched with the carboxylic acid fucntionalities. CNDs exhibited a fluorescence emission at 445 nm and unveiled blue luminescence in ultraviolet excitation. Read More

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http://dx.doi.org/10.1007/s10895-019-02360-wDOI Listing
April 2019
1.927 Impact Factor

Report on the AAPS Immunogenicity Guidance Forum.

AAPS J 2019 Apr 16;21(4):55. Epub 2019 Apr 16.

Pharmaceutical Consultant, North Potomac, Maryland, 20878, USA.

In September 2018, the American Association of Pharmaceutical Scientists (AAPS) conducted an Annual Guidance Forum on the considerations related to immunogenicity testing for therapeutic protein products. In addition to a broad representation by the pharmaceutical industry, the event included strong representation by leading scientists from the US Food and Drug Administration (FDA). The agency and industry perspectives and updates to the guidance were presented. Read More

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http://link.springer.com/10.1208/s12248-019-0328-8
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http://dx.doi.org/10.1208/s12248-019-0328-8DOI Listing
April 2019
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Two data pre-processing workflows to facilitate the discovery of biomarkers by 2D NMR metabolomics.

Metabolomics 2019 Apr 16;15(4):63. Epub 2019 Apr 16.

Center for Interdisciplinary Research on Medicines (CIRM), Metabolomics group, Université de Liège (ULg), Liege, Belgium.

Introduction: The pre-processing of analytical data in metabolomics must be considered as a whole to allow the construction of a global and unique object for any further simultaneous data analysis or multivariate statistical modelling. For 1D H-NMR metabolomics experiments, best practices for data pre-processing are well defined, but not yet for 2D experiments (for instance COSY in this paper).

Objective: By considering the added value of a second dimension, the objective is to propose two workflows dedicated to 2D NMR data handling and preparation (the Global Peak List and Vectorization approaches) and to compare them (with respect to each other and with 1D standards). Read More

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http://dx.doi.org/10.1007/s11306-019-1524-3DOI Listing

Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides.

Anal Bioanal Chem 2019 Apr 16. Epub 2019 Apr 16.

CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, Liaoning, China.

Because of structural flexibility and acid lability, the identification of phosphorylated lysine (pLys) peptides is a great challenge. We report here a cleavable hydrophobic derivatization (CHD) strategy for the enrichment and identification of pLys peptides. First, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate was synthesized to react with dephosphorylated lysine peptides, and then the derived peptides were captured by a C column, followed by cleavage of the hydrophobic chain, with the specific label left on the target peptides for further identification. Read More

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http://dx.doi.org/10.1007/s00216-019-01770-wDOI Listing

A multifunctional persistent luminescent nanoprobe for imaging guided dual-stimulus responsive and triple-synergistic therapy of drug resistant tumor cells.

Chem Commun (Camb) 2019 Apr 17. Epub 2019 Apr 17.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. and International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

We report the design and fabrication of a multifunctional persistent luminescent nanoprobe for imaging guided dual-stimulus responsive and triple-synergistic therapy of drug resistant tumor. The integration of the dual-stimulus of an acidic microenvironment and laser irradiation with the triple-synergistic therapy of doxorubicin, photothermal and siRNA offers excellent therapeutic performance for drug resistant tumor cells with high specificity and little side effects. Read More

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http://dx.doi.org/10.1039/c9cc01446bDOI Listing

Data on validation using accuracy profile of HPLC-UV method.

Data Brief 2019 Jun 26;24:103877. Epub 2019 Mar 26.

Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

The data presented are related to the article entitled "Six Sigma quality approach for HPLC-UV method optimization" Ibrahim et al., 2019. The raw data of HPLC analysis of ascorbic acid (AS), paracetamol (PA) and guaifenesin (GU) are presented. Read More

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http://dx.doi.org/10.1016/j.dib.2019.103877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449764PMC

Comparing the participation of men and women in academic medicine in medical colleges in Sudan: A cross-sectional survey.

J Educ Health Promot 2019 15;8:31. Epub 2019 Feb 15.

Department of Medicine and HIV Metabolic Clinic, Milton Keynes University Hospital NHS Foundation Trust, Milton Keynes, Buckinghamshire, UK.

Introduction: In different countries around the world, the involvement of women in academic medicine was less in comparison with men. This study aimed to assess whether there were significant gender differences in research perception, practice, and publication in Sudan.

Methods: This was an analytical cross-sectional study was carried out using questionnaire among 153 teaching staff of five Sudanese medical faculties from both genders, including teaching assistants, lecturers, assistant professors, associate professors, and full professors. Read More

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http://dx.doi.org/10.4103/jehp.jehp_225_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432818PMC
February 2019

A systematic simulation-based meta-analytical framework for prediction of physiological biomarkers in alopecia.

J Biol Res (Thessalon) 2019 Dec 4;26. Epub 2019 Apr 4.

5Informatics Institute, School of Medicine, The University of Alabama (UAB), Birmingham, USA.

Background: Alopecia or hair loss is a complex polygenetic and psychologically devastating disease affecting millions of men and women globally. Since the gene annotation and environmental knowledge is limited for alopecia, a systematic analysis for the identification of candidate biomarkers is required that could provide potential therapeutic targets for hair loss therapy.

Results: We designed an interactive framework to perform a meta-analytical study based on differential expression analysis, systems biology, and functional proteomic investigations. Read More

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http://dx.doi.org/10.1186/s40709-019-0094-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449998PMC
December 2019

Fast Determination of Auramine O in Food by Adsorptive Stripping Voltammetry.

J Anal Methods Chem 2019 12;2019:8639528. Epub 2019 Mar 12.

Department of Analytical Chemistry, Faculty of Chemistry, VNU University of Science, 19 Le Thanh Tong Street, Hanoi, Vietnam.

The electrochemical behaviour of auramine O on the hanging mercury drop electrode has been investigated by cyclic and square wave voltammetry method. Reduction peak of auramine O was irreversible and adsorptive on the hanging mercury drop electrode. The optimal conditions were chosen to be Briton-Robinson buffer pH 9. Read More

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http://dx.doi.org/10.1155/2019/8639528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434281PMC

Ranking network mechanisms by how they fit diverse experiments and deciding on 's ammonium transport and assimilation network.

NPJ Syst Biol Appl 2019 12;5:14. Epub 2019 Apr 12.

3Department of Molecular Cell Biology, Faculty of Science, VU University Amsterdam, O|2 building, Amsterdam, Netherlands.

The complex ammonium transport and assimilation network of involves the ammonium transporter AmtB, the regulatory proteins GlnK and GlnB, and the central N-assimilating enzymes together with their highly complex interactions. The engineering and modelling of such a complex network seem impossible because functioning depends critically on a gamut of data known at patchy accuracy. We developed a way out of this predicament, which employs: (i) a constrained optimization-based technology for the simultaneous fitting of models to heterogeneous experimental data sets gathered through diverse experimental set-ups, (ii) a 'rubber band method' to deal with different degrees of uncertainty, both in experimentally determined or estimated parameter values and in measured transient or steady-state variables (training data sets), (iii) integration of human expertise to decide on accuracies of both parameters and variables, (iv) massive computation employing a fast algorithm and a supercomputer, (v) an objective way of quantifying the plausibility of models, which makes it possible to decide which model is the best and how much better that model is than the others. Read More

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http://dx.doi.org/10.1038/s41540-019-0091-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461619PMC

An improved synthesis of adefovir and related analogues.

Beilstein J Org Chem 2019 29;15:801-810. Epub 2019 Mar 29.

School of Chemistry, University College Cork, Cork, Ireland.

An improved synthesis of the antiviral drug adefovir is presented. Problems associated with current routes to adefovir include capricious yields and a reliance on problematic reagents and solvents, such as magnesium -butoxide and DMF, to achieve high conversions to the target. A systematic study within our laboratory led to the identification of an iodide reagent which affords higher yields than previous approaches and allows for reactions to be conducted up to 10 g in scale under milder conditions. Read More

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https://www.beilstein-journals.org/bjoc/articles/15/77
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http://dx.doi.org/10.3762/bjoc.15.77DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444443PMC
March 2019
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Hybrid Degradation Equipment Remaining Useful Life Prediction Oriented Parallel Simulation considering Model Soft Switch.

Comput Intell Neurosci 2019 12;2019:9179870. Epub 2019 Mar 12.

Shijiazhuang Campus, Army Engineering University, Shijiazhuang 050003, China.

Equipment parallel simulation is an emerging simulation technology in recent years, and equipment remaining useful life (RUL) prediction oriented parallel simulation is an important branch of parallel simulation. An important concept in equipment parallel simulation is the model evolution driven by real-time data, including model selection and model parameter evolution. The current research on equipment RUL prediction oriented parallel simulation mainly focuses on a single continuous degradation mode, such as linear degradation and nonlinear degradation. Read More

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http://dx.doi.org/10.1155/2019/9179870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434305PMC

Intrinsic insular-frontal networks predict future nicotine dependence severity.

J Neurosci 2019 Apr 16. Epub 2019 Apr 16.

Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural Research Program, 251 Bayview Blvd, Baltimore, MD, 21224

Although 60% of the US population have tried smoking cigarettes, only 16% smoke regularly. Identifying this susceptible subset of the population before the onset of nicotine dependence may encourage targeted early interventions to prevent regular smoking and/or minimize severity. While prospective neuroimaging in human populations can be challenging, preclinical neuroimaging models prior to chronic nicotine administration can help develop translational biomarkers of disease risk. Read More

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http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0140-1
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http://dx.doi.org/10.1523/JNEUROSCI.0140-19.2019DOI Listing
April 2019
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What enables older people to continue with their falls prevention exercises? A qualitative systematic review.

BMJ Open 2019 Apr 15;9(4):e026074. Epub 2019 Apr 15.

RCN RI, Royal College of Nursing Research Institute, Warwick Medical School, University of Warwick, Coventry, UK.

Objectives: To review the qualitative literature that explores the barriers and facilitators to continued participation in falls prevention exercise after completion of a structured exercise programme.

Design: A systematic literature review with thematic synthesis of qualitative studies exploring older adults' experiences of continued participation in falls prevention exercise.

Data Sources: Comprehensive searches were conducted in MEDLINE, PSYCHinfo, AMED, ASSIA, CINAHL and EMBASE from inception until November 2017. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-026074DOI Listing

Correlation between the results of two analytical methods for measuring measles virus neutralizing antibodies in source plasma and therapeutic immunoglobulin products.

Biologicals 2019 Apr 13. Epub 2019 Apr 13.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 02841, Anam-dong, Seoungbuk-gu, Seoul, Republic of Korea. Electronic address:

Patients with primary immunodeficiency disorders are vulnerable to infectious diseases. Intravenous immunoglobulin (IVIG) therapeutic products manufactured from human plasma are employed widely to protect patients from pathogens such as measles virus, which causes a potentially fatal and contagious disease. Therefore, health authorities stipulate a minimum titer of measles neutralizing antibodies (mnAbs) in IVIG products to ensure efficient protection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10451056193002
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http://dx.doi.org/10.1016/j.biologicals.2019.03.009DOI Listing
April 2019
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The Effect of an Audiovisual Preoperative Intervention in Reducing Parental Anxiety Prior to a Child's Sedation Appointment: A Pilot Study.

Pediatr Dent 2019 Mar;41(2):112-118

Dr. Mathu-Muju is associate professor of pediatric dentistry, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada;, Email:

The purpose of this pilot study was to determine whether an audiovisual intervention was more effective than verbal instructions at reducing preoperative anxiety levels for parents whose children were having their first experience of dental treatment with the use of oral sedation. A prospective clinical trial was conducted. Parents were systematically assigned to either view an animated video (intervention group) or receive standardized verbal instructions (control group). Read More

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March 2019
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2-Monochloropropanediol (2-MCPD), 3-Monochloropropanediol (3-MCPD), and Glycidol in Infant and Adult/Pediatric Nutritional Formula: Single-Laboratory Validation, First Action 2018.12.

Authors:
Jan Kuhlmann

J AOAC Int 2019 Apr 16. Epub 2019 Apr 16.

SGS Germany GmbH, Weidenbaumsweg 137, D-21035 Hamburg, Germany.

Fatty acid esters of glycidol, 2-Monochloropropanediol (MCPD), and 3-MCPD are heat-induced foodborne processing contaminants with possible adverse health effects. These compounds occur frequently in refined edible oils. Consequently, glycidyl esters and 2- and 3-MCPD esters might also be present in foods that contain refined edible oils. Read More

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http://dx.doi.org/10.5740/jaoacint.19-0026DOI Listing

Bactericidal effects and accelerated wound healing using TbO nanoparticles with intrinsic oxidase-like activity.

J Nanobiotechnology 2019 Apr 16;17(1):54. Epub 2019 Apr 16.

Division of Analytical Chemistry, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, 20740, USA.

Background: Nanomaterials that exhibit intrinsic enzyme-like characteristics have shown great promise as potential antibacterial agents. However, many of them exhibit inefficient antibacterial activity and biosafety problems that limit their usefulness. The development of new nanomaterials with good biocompatibility and rapid bactericidal effects is therefore highly desirable. Read More

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http://dx.doi.org/10.1186/s12951-019-0487-xDOI Listing

Estimation of early life endogenous surfactant pool and CPAP failure in preterm neonates with RDS.

Respir Res 2019 Apr 16;20(1):75. Epub 2019 Apr 16.

Division of Pediatrics and Neonatal Critical Care, Medical Center "A.Béclère", South Paris University Hospitals, AP-HP, Paris, France.

Background: It is not known if the endogenous surfactant pool available early in life is associated with the RDS clinical course in preterm neonates treated with CPAP. We aim to clarify the clinical factors affecting surfactant pool in preterm neonates and study its association with CPAP failure.

Methods: Prospective, pragmatic, blind, cohort study. Read More

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http://dx.doi.org/10.1186/s12931-019-1040-zDOI Listing

Preferences for aspects of antenatal and newborn screening: a systematic review.

BMC Pregnancy Childbirth 2019 Apr 16;19(1):131. Epub 2019 Apr 16.

Manchester Centre for Health Economics, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK.

Background: Many countries offer screening programmes to unborn and newborn babies (antenatal and newborn screening) to identify those at risk of certain conditions to aid earlier diagnosis and treatment. Technological advances have stimulated the development of screening programmes to include more conditions, subsequently changing the information required and potential benefit-risk trade-offs driving participation. Quantifying preferences for screening programmes can provide programme commissioners with data to understand potential demand, the drivers of this demand, information provision required to support the programmes and the extent to which preferences differ in a population. Read More

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http://dx.doi.org/10.1186/s12884-019-2278-7DOI Listing

Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation.

Int J Mol Sci 2019 Apr 15;20(8). Epub 2019 Apr 15.

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang 11800, Malaysia.

Antibodies leverage on their unique architecture to bind with an array of antigens. The strength of interaction has a direct relation to the affinity of the antibodies towards the antigen. affinity maturation is performed through multiple rounds of somatic hypermutation and selection in the germinal centre. Read More

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http://dx.doi.org/10.3390/ijms20081861DOI Listing

International Study of Childhood Obesity, Lifestyle and the Environment (ISCOLE): Contributions to Understanding the Global Obesity Epidemic.

Nutrients 2019 Apr 15;11(4). Epub 2019 Apr 15.

Department of Kinesiology, University of Massachusetts, Amherst, MA 01003, USA.

The purpose of this review is to summarize the scientific contributions of the International Study of Childhood Obesity, Lifestyle and the Environment (ISCOLE) in extending our understanding about obesity in children from around the world. ISCOLE was a multi-national study of 9 to 11 year-old children from sites in 12 countries from all inhabited continents. The primary purpose was to investigate relationships between lifestyle behaviors and obesity, and the influence of higher-order characteristics such as behavioral settings, and physical, social and policy environments. Read More

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http://dx.doi.org/10.3390/nu11040848DOI Listing