26,878 results match your criteria Analytical biochemistry[Journal]


A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.

Anal Biochem 2019 Apr 19. Epub 2019 Apr 19.

Dr. BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, 110007, India. Electronic address:

In general, 4 amino-5-methylamino-2',7'-difluorescein diacetate (DAF-FM-DA) dye is used to detect nitric oxide in biological systems through cell imaging. In this study, we have used 96 well plate format to quantify nitric oxide using DAF-FM-DA through a multimode reader (or independently using fluorospectrometer) and could be visualized in fluorescence microscope. Similar study otherwise will require a high-end instrument. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.014DOI Listing

Template-ready PCR method for detection of human telomerase reverse transcriptase mRNA in sputum.

Anal Biochem 2019 Apr 13. Epub 2019 Apr 13.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, 310003, PR China. Electronic address:

Human telomerase reverse transcriptase (hTERT) mRNA in tissue is a biomarker of lung cancer, but hTERT mRNA in sputum had not been successfully detected with conventional reverse transcription PCR methods. Here, we developed a novel PCR protocol: Template-Ready PCR (TRPCR), to detect sputum hTERT mRNA, in which probes serve as templates of amplification. While free probes and dsDNA were removed in template preparation through aspiration and restriction digestion, probes that formed into heterocomplex with target RNA remained intact for PCR amplification. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697193024
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.04.008DOI Listing
April 2019
2 Reads

Rapid detection of insulin by immune-enrichment with silicon-nanoparticle-assisted MALDI-TOF MS.

Anal Biochem 2019 Apr 13;577:14-20. Epub 2019 Apr 13.

Department of Urology, The Fifteenth Military Hospital of China, Wusu, Xinjiang, 833000, China. Electronic address:

Background: Insulin is central to regulating fat and carbohydrate metabolism in the body. However, it is difficult to detect insulin using mass spectrometry (MS). The integration of nanotechnology with mass spectrometry for selective and sensitive detection is an important research area. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697193026
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.04.010DOI Listing
April 2019
1 Read

Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.

Anal Biochem 2019 Apr 11;577:1-13. Epub 2019 Apr 11.

Department of Physiology and Cell Biology, University of Nevada Reno, School of Medicine, MS 0352, 1664 N Virginia St, Reno, NV, 89557, USA. Electronic address:

Antibody-based in situ proximity ligation assays (isPLA) have the potential to study protein phosphorylation and protein interactions with spatial resolution in intact tissues. However, the application of isPLA at the tissue level is limited by a lack of appropriate positive and negative controls and the difficulty in accounting for changes in tissue shape. Here we demonstrate a set of experimental and computational approaches using gastric fundus smooth muscles to improve the validity of quantitative isPLA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.009DOI Listing

Crossly charged microfluidic device for spontaneous filtration without an external power supply.

Anal Biochem 2019 Apr 11;577:21-25. Epub 2019 Apr 11.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea. Electronic address:

Spontaneous filtration without a power supply was investigated using highly charged precursor mixtures instead of recognized microfluidic desalination techniques. The proposed filtration method consisted of a main channel (depth: 1 mm) and shallow channels (depth: 400 μm) in which the precursor mixture was injected. The filtration performance of the proposed device was evaluated by injecting a fluorescent dye that moved toward the centerline of the main channel. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.007DOI Listing

A sample processing method for immunoassay of whole blood tacrolimus.

Anal Biochem 2019 Apr 10;576:13-19. Epub 2019 Apr 10.

Shanghai Genext Medical Technology Co., Ltd, Shanghai, 200433, China.

Current sample processing (SP) methods for tacrolimus (FK506) immunoassays are mainly based on extraction of drug by organic solvent and divalent metal ions. Although these methods are effective for drug extraction and interference elimination, they suffer from drawbacks including inconvenience for operation, difficulties for automation and potential measurement bias. To overcome these limitations, this study describes a new SP reagent for blood cell lysis and protein denaturation. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697193017
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.04.006DOI Listing
April 2019
2 Reads

A method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of Aedes aegypti mosquitoes.

Anal Biochem 2019 Apr 8;576:33-41. Epub 2019 Apr 8.

Laboratório de Bioquímica de Resposta Ao Estresse, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal Do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia Em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. Electronic address:

Aedes aegypti is the most important and widespread vector of arboviruses, including dengue and zika. Insect dispersal through the flight activity is a key parameter that determines vector competence, and is energetically driven by oxidative phosphorylation in flight muscle mitochondria. Analysis of mitochondrial function is central for a better understanding of cellular metabolism, and is mostly studied using isolated organelles. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.005DOI Listing

Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers.

Anal Biochem 2019 Apr 8;576:42-47. Epub 2019 Apr 8.

Yantai Quanticision Diagnostics Inc., A Division of Quanticision Diagnostics, Inc. (US), Yantai Hi-tech District, Shandong, 264670, PR China; Medicine and Pharmarcy Research Center, Precision Medicine Division, Binzhou Medical University, Laishan District, Shandong, 264003, PR China. Electronic address:

To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in the analysis. The protein levels of four breast cancer tissue biomarkers, including Estrogen Receptor (ER), Progesterone Receptor (PR), Ki67 and Her2, were absolutely quantitated successfully in 190 frozen breast tissue biopsies, and the results were further verified with provided IHC results. We propose QDB method as an alternative platform to Sandwich ELISA for absolute quantitation of tissue biomarkers with significantly reduced developing effort and time. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.003DOI Listing
April 2019
1 Read
2.219 Impact Factor

NMR metabolomics for evaluating passage number and harvesting effects on mammalian cell metabolome.

Anal Biochem 2019 Apr 7;576:20-32. Epub 2019 Apr 7.

Singapore Centre for Environmental Life Science Engineering, National University of Singapore, Singapore.

The variation in the extracellular metabolites of RAW 264.7 cells obtained from different passage numbers (passage 9, 12 and 14) was examined. The impact of different harvesting protocols (trypsinization and scraping) on recovery of intracellular metabolites was then assessed. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697183121
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.04.001DOI Listing
April 2019
4 Reads

Fluorescent determination of micro-quantities of RNA using Hoechst 33258 and binase.

Anal Biochem 2019 Apr 5;576:5-8. Epub 2019 Apr 5.

Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia. Electronic address:

Detection of small amounts of RNA in various biological samples is an important applied task. Using fluorescence spectroscopy, the hydrolysis by binase of rRNA and tRNA, stained with Hoechst 33258, in aqueous solutions was investigated. The binding constant of Hoechst with rRNA is 10 M. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.002DOI Listing
April 2019
1 Read

Efficient gene disruption by posttransformational directed internal homologous recombination in Pichia pastoris.

Anal Biochem 2019 Apr 6;576:1-4. Epub 2019 Apr 6.

Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China. Electronic address:

In this study, we designed an improved method to construct gene-deficient strain in non-conventional yeast Pichia pastoris. This method achieved high efficiency of gene deletion without disruption of NHEJ-related genes. The disruption efficiency of Och1 reached about 80%. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.04.004DOI Listing

Characterization of H2S releasing properties of various H2S donors utilizing microplate cover-based colorimetric assay.

Anal Biochem 2019 Jun 1;574:57-65. Epub 2019 Apr 1.

Dept. of Medical Engineering, Graduate School, Kyung Hee University, Seoul, 130-701, South Korea; Dept. of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, 130-701, South Korea. Electronic address:

In this study, we characterized the potential HS-releasing properties of seven different HS donors, including sodium sulfide (NaS), sodium hydrosulfide (NaHS), diallyl disulfide (DADS), diallyl trisulfide (DATS), sodium thiosulfate (NaSO), morpholin-4-ium 4-methoxyphenyl-morpholino-phosphinodithioate (GYY4137), and Lawesson's reagent, in three assay solutions, phosphate buffered saline (PBS, pH 7.4), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffered saline (HBS, pH 7.6), and cell growth media (GM), utilizing our microplate cover-based colorimetric assay. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.021DOI Listing
June 2019
1 Read

Development of an HPLC-based guanosine monophosphate kinase assay and application to Plasmodium vivax guanylate kinase.

Anal Biochem 2019 Mar 31;575:63-69. Epub 2019 Mar 31.

Griffith Institute for Drug Discovery, Griffith University, Brisbane, Queensland, Australia. Electronic address:

The development of a high-performance liquid chromatography (HPLC)-based method, for guanosine monophosphate kinase activity assays, is presented. The method uses the intrinsic UV absorption (at 260 nm) of substrates and products of the enzymatic reaction (GMP, ATP, ADP and GDP) to unambiguously determine percent conversion of substrate into product. It uses a commercially available C18 column which can separate reaction samples by elution under isocratic conditions in 12 min per run. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.022DOI Listing
March 2019
1 Read

Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization.

Anal Biochem 2019 Mar 30;575:40-43. Epub 2019 Mar 30.

Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, 08903, USA; Department of Medicine, Rutgers-Robert Wood Johnson Medical School, New Brunswick, NJ, 08901, USA. Electronic address:

Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697183127
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.03.020DOI Listing
March 2019
2 Reads

Development of duplex-crossed allele-specific PCR targeting of TPMT*3B and *3C using crossed allele-specific blockers to eliminate non-specific amplification.

Anal Biochem 2019 Mar 30;575:54-62. Epub 2019 Mar 30.

Department of Laboratory Medicine, Daping Hospital, Third Military Medical University(Army Medical University), Chongqing, 400042, PR China; Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University(Army Medical University), Chongqing, 400038, PR China. Electronic address:

Prospective testing for variants in the thiopurine S-methyltransferase (TPMT) is considered a key process in the development of thiopurine therapy. This testing is done to avoid toxicity and side effects in the management of diverse immunological and malignant conditions. Real-time fluorescent PCR techniques using duplex-crossed allele-specific primers in a single tube (DCAS-PCR) were developed in this study to genotype the common loss-of-function TPMT*3B c. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.019DOI Listing

iMotor-CNN: Identifying molecular functions of cytoskeleton motor proteins using 2D convolutional neural network via Chou's 5-step rule.

Anal Biochem 2019 Mar 28;575:17-26. Epub 2019 Mar 28.

Medical Humanities Research Cluster, School of Humanities, Nanyang Technological University, 48 Nanyang Ave, 639798, Singapore. Electronic address:

Motor proteins are the driving force behind muscle contraction and are responsible for the active transportation of most proteins and vesicles in the cytoplasm. There are three superfamilies of cytoskeletal motor proteins with various molecular functions and structures: dynein, kinesin, and myosin. The functional loss of a specific motor protein molecular function has linked to a variety of human diseases, e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.017DOI Listing
March 2019
2 Reads
2.219 Impact Factor

Betaine-assisted recombinase polymerase assay with enhanced specificity.

Anal Biochem 2019 Mar 29;575:36-39. Epub 2019 Mar 29.

Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China. Electronic address:

Recombinase polymerase amplification (RPA) is a widespread isothermal amplification method and regarded as an excellent candidate to replace polymerase chain reaction. However, the specificity of RPA is not always satisfactory when the sample contains amounts of background DNA. Herein, we report a novel RPA method named betaine-assisted RPA (B-RPA) that uses inexpensive betaine to avoid nonspecific amplification effectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.018DOI Listing

High affinity of AS1411 toward copper; its application in a sensitive aptasensor for copper detection.

Anal Biochem 2019 Mar 26;575:1-9. Epub 2019 Mar 26.

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

AS1411 is a 26-mer G-rich DNA aptamer which has been broadly employed in the field of targeted drug delivery, due to its capability to bind to nucleolin protein on the surface of cancer cells. In this work, it has been shown for the first time that in addition to nucleolin, AS1411 aptamer could bind to copper ions (Cu) with high affinity and selectivity, affecting AS1411 usage in drug delivery systems as a targeting agent. In this study, besides the evaluations of the affinity of AS1411 to different ions and the impact of Cu on targeted drug delivery employed AS1411 aptamer as a targeting agent, a simple and ultra-sensitive fluorescent aptasensor was fabricated for Cu detection through applying AS1411, its complementary strand and magnetic beads coated with streptavidin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.016DOI Listing
March 2019
1 Read

A fast and simple headspace gas chromatographic technique for quantitatively analyzing urea in human urine.

Anal Biochem 2019 Mar 24;576:9-12. Epub 2019 Mar 24.

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.

A fast and convenient headspace gas chromatographic (HS-GC) approach was described for the estimation of urea in human urine. The HS-GC could detect the generated carbon dioxide derived from the urease-catalyzed hydrolysis of urea. It was found that the hydrolysis of urea catalyzed by urease was completed within 40 min at 35 °C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.011DOI Listing
March 2019
1 Read

Approaches for probing and evaluating mammalian sphingolipid metabolism.

Anal Biochem 2019 Mar 24;575:70-86. Epub 2019 Mar 24.

Department of Medicine, Stony Brook University, Stony Brook, NY, USA; The Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY, USA; Departments of Biochemistry, Pathology and Pharmacology, Stony Brook University, Stony Brook, NY, USA. Electronic address:

Sphingolipid metabolism plays a critical role in regulating processes that control cellular fate. This dynamic pathway can generate and degrade the central players: ceramide, sphingosine and sphingosine-1-phosphate in almost any membrane in the cell, adding an unexpected level of complexity in deciphering signaling events. While in vitro assays have been developed for most enzymes in SL metabolism, these assays are setup for optimal activity conditions and can fail to take into account regulatory components such as compartmentalization, substrate limitations, and binding partners that can affect cellular enzymatic activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.014DOI Listing

Extraction of plasmid DNA by use of a magnetic maghemite-polyaniline nanocomposite.

Anal Biochem 2019 Mar 24;575:27-35. Epub 2019 Mar 24.

Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil; Departamento de Física, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil. Electronic address:

We describe the use of a hybrid magnetic nanocomposite (HMNC) for the extraction and purification of plasmid DNA (pDNA) from Escherichia coli aqueous solutions. The HMNC, which was synthesized via emulsion polymerization, was characterized by transmission electron microscopy, scanning electron microscopy, UV-Vis spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering and magnetic measurements. The results confirmed the incorporation of polyaniline (Pani) in its conducting form onto a core formed by the magnetic iron oxide, with the hybrid particles presenting an average size of (95 ± 30) nm and a saturation magnetization of 30 emu/g. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.013DOI Listing

Differential potential ratiometric sensing platform for enantiorecognition of chiral drugs.

Anal Biochem 2019 Jun 23;574:39-45. Epub 2019 Mar 23.

College of Materials Science and Engineering, Huaqiao University, Key Laboratory of Molecular Designing and Green Conversions Fujian University, Xiamen, 361021, People's Republic of China.

A versatile, robust and efficient differential potential ratiometric sensing platform was developed for enantioselective recognition of dual chiral targets based on a composite membrane of molecularly imprinted polymers (MIPs) and reduced graphene oxide (rGO) modified glassy carbon electrode (GCE). The functional chitosan-based MIPs and rGO were compatibly immobilized on the GCE with high selectivity and efficient signal amplification. Moreover, via the systematic optimization of series conditions, a distinct potential difference (PD), reaching 135 mV, was obtained between the R-/S-prop based on the MIPs/rGO/GCE. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.015DOI Listing

Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src.

Anal Biochem 2019 Jun 23;574:46-56. Epub 2019 Mar 23.

Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, M1C 1A4, Canada; Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6, Canada. Electronic address:

Nicotinic acetylcholine receptors (nAChR) are ion channels which regulate a numerous of neurotransmitters, including acetylcholine, norepinephrine, dopamine, serotonin and glutamate. These receptors are important targets for the study of a plethora of diseases such as Alzheimer's disease, schizophrenia, Parkinson's Disease, cancer, inflammation, etc. The α7 subunits are especially interesting in that they are commonly occurring and are critical sites of regulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.012DOI Listing

Ponceau S waste: Ponceau S staining for total protein normalization.

Anal Biochem 2019 Mar 23;575:44-53. Epub 2019 Mar 23.

Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA, USA; Department of Physiology and Membrane Biology, University of California, Davis, CA, USA. Electronic address:

Normalization of Western blotting data is a critical step that is needed to reduce errors caused by unequal sample loading across lanes in a gel, inconsistent sample preparation, and variations due to experimental errors. Several papers have suggested that total protein normalization may be better than housekeeping protein normalization for Western blotting normalization. Ponceau S is the most commonly used stain for total protein normalization. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.010DOI Listing

Site-specific observation of the conformational change of a protein with N-labeled Tyr residues using NMR.

Anal Biochem 2019 Jun 23;574:34-38. Epub 2019 Mar 23.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto 606-8522, Japan. Electronic address:

One of the reasons it is difficult to analyze protein structural dynamics at atomic resolution using NMR is the molecular size of the protein. The selective amino acid labeling method is one of the effective methods that can solve this problem. In this study, to determine the site-specific conformational change in 3α-hydroxysteroid dehydrogenase from Pseudomonas sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.009DOI Listing

Tridimensional alginate disks of tunable topologies for mammalian cell encapsulation.

Anal Biochem 2019 Jun 21;574:31-33. Epub 2019 Mar 21.

Department of Biomedical Engineering, Universidad de los Andes, Bogotá, Colombia. Electronic address:

We have developed a protocol to produce three-dimensional matrices based on alginate hydrogels for mammalian cell encapsulation. Based on the gelation properties of this polysaccharide, we implemented a calcium ion-based diffusion method where the designed hydrogels can be obtained with well-defined mechanical properties and replicable 3D topologies. The developed protocol can be extended to different types of alginates and an ample range of concentrations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.008DOI Listing
June 2019
3 Reads

Spectrophotometric method for simultaneous measurement of zinc and copper in metalloproteins using 4-(2-pyridylazo)resorcinol.

Anal Biochem 2019 Mar 20. Epub 2019 Mar 20.

Department of Chemistry, University of Waterloo, Waterloo, Canada. Electronic address:

Bound metals are observed in a great many natural proteins, where they perform diverse roles in determining protein folding, stability and function. Due to the diverse impact of bound metals on biophysical and biochemical properties of proteins, it is valuable to have accurate and facile methods for determining the metal content of proteins. Here we describe an optimized methodology using 4-(2-pyridylazo)resorcinol (PAR) to simultaneously quantify two metal ions in solution. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.007DOI Listing

A dual amplification electrochemical immunosensor based on HRP-Au@Ag NPs for carcinoembryonic antigen detection.

Anal Biochem 2019 Jun 20;574:23-30. Epub 2019 Mar 20.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China. Electronic address:

A sensitive sandwich-type electrochemical immunosensor based on dual amplification strategy was constructed. The dual amplification strategy has been used secondary antibody(Ab)-horseradish peroxidase(HRP)-Au@Ag nanoparticles (Au@Ag NPs) for carcinoembryonic antigen(CEA) detection. Ab-HRP-Au@Ag NPs as dual amplification markers triggered the disproportionation of HO, which could facilitate the catalytic oxidation of hydroquinone to quinone(BQ). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.003DOI Listing

Quantification of proteins in whole blood, plasma and DBS, with element-labelled antibody detection by ICP-MS.

Anal Biochem 2019 Mar 20;575:10-16. Epub 2019 Mar 20.

Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK. Electronic address:

Over recent years, quantification of multiple proteins in body fluids has become increasingly prominent, which is beneficial to a number of scientific fields, not least biomedical. Several techniques have been developed based on conventional ELISA; one of these techniques is analysis of proteins labelled with element-tagged antibodies by ICP-MS in serum, allowing quantification of multiple targets within a single sample. This research aimed to quantify albumin and immunoglobulin G (IgG) levels in plasma, whole blood and dried blood spots using NANOGOLD and Europium labelled antibodies analysed by ICP-MS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.006DOI Listing

HPLC method for the assessment of tryptophan metabolism utilizing separate internal standard for each detector.

Anal Biochem 2019 Jun 15;574:7-14. Epub 2019 Mar 15.

Department of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged, Hungary. Electronic address:

The development of a validated method, applicable for the measurement of tryptophan (TRP) and serotonin (5-HT), and that of the neuroprotective branch of the kynurenine pathway from several different biological matrices, including mouse brain, is described. Following the spectral analysis of the metabolites, they were quantified with reversed-phase high-performance liquid chromatography (HPLC), using separate internal standards (ISs) for UV (3-nitro-L-tyrosine) and fluorescent (the newly utilized 4-hydroxyquinazoline-2-carboxylic acid) detectors. With regard to validation parameters, selectivity, linearity, limit of detection, limit of quantification, precision and recovery were determined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.005DOI Listing

A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma.

Anal Biochem 2019 Jun 14;574:15-22. Epub 2019 Mar 14.

The Antibody Lab GmbH, Divischgasse 4, A-1210, Vienna, Austria.

Human semaphorin 4D (SEMA4D), a type I integral membrane glycoprotein, regulates key cellular functions (e.g. cell-cell communication, platelet activation). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.03.004DOI Listing
June 2019
1 Read

A novel visual-mixed-dye for LAMP and its application in the detection of foodborne pathogens.

Anal Biochem 2019 Jun 9;574:1-6. Epub 2019 Mar 9.

Department of Hygienic Inspection, School of Public Health, Jilin University, Changchun, 130021, PR China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, PR China. Electronic address:

Loop-mediated isothermal amplification (LAMP) has been widely applied for the detection of foodborne pathogens. Obtaining a simple, accurate readout of LAMP reaction results is crucial. Herein, a visual-mixed-dye (VMD) containing calcein (precombined with MnCl) and hydroxynaphthol blue (HNB) for LAMP end-point detection was developed. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697193010
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.03.002DOI Listing
June 2019
7 Reads

An electrochemical dual-signaling aptasensor for the ultrasensitive detection of insulin.

Anal Biochem 2019 May 9;573:30-36. Epub 2019 Mar 9.

Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, 100850, PR China. Electronic address:

Insulin plays a central role in physiological glycolmetabolism and is associated with diabetes and related diseases. In this work, a dual-signaling electrochemical aptasensor for insulin detection with high sensitivity and specificity has been reported. Methylene blue (MB)-modified insulin-binding aptamer (IBA) as "signal-off" probe, and (DNA2)/Ferrocene (Fc) co-modified gold nanoparticles (DNA2Fc@GNPs) as the "signal-on" probe were integrated with linker mDNA to fabricate the DNA2Fc@GNPs/mDNA/MB-IBA modified Au electrode as the sensing interface, and the current responses of MB and Fc were used as signal indicators. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.032DOI Listing
May 2019
1 Read

Quantitation of low concentrations of polysorbates 80 in protein formulations by Coomassie brilliant blue.

Anal Biochem 2019 May 7;573:67-72. Epub 2019 Mar 7.

Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China; Institue of Immunology, University of Science and Technology of China, Hefei, Anhui, China. Electronic address:

Polysorbate 80, as pharmaceutical excipient and virus inactivating agent, is commonly used in the protein pharmaceutical industry. In this study, a method has been developed for the determination of low concentration of Polysorbate 80 in the presence of high concentration proteins (≤100 mg/ml) and excipients. This colorimetric method is based on the interaction of Polysorbate 80 and Coomassie brilliant blue, and suitable for quantitation of Polysorbate 80 in the range of 10-100 μg/ml. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697183021
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.03.001DOI Listing
May 2019
8 Reads

Selected lipid-based transfection reagents activate NF-κB and MAP kinases signaling pathways, induced cytokines mRNA expression in human THP-1 macrophage.

Anal Biochem 2019 May 7;573:73-76. Epub 2019 Mar 7.

Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, ARS, U.S. Department of Agriculture, Beltsville, MD, 20705, USA. Electronic address:

In the present study, we evaluated, under transient transfection conditions, five different cationic lipid-based transfection reagents on the activation of NF-κB, MAP kinases signaling pathways and induction of cytokines expression. We found that the reagents studied differentially regulated the NF-κB and the MAP kinases signaling pathways in the human THP-1 macrophage. Additionally, mRNA expression levels of the cytokines, IL-1β and TNF-α in THP-1 macrophage were also induced by selected test reagents. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697183121
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.02.018DOI Listing
May 2019
6 Reads

Split luciferase-based assay for simultaneous analyses of the ligand concentration- and time-dependent recruitment of β-arrestin2.

Anal Biochem 2019 May 8;573:8-16. Epub 2019 Mar 8.

Institute of Pharmacy, University of Regensburg, Universitätsstraße 31, D-93053, Regensburg, Germany. Electronic address:

Functional selectivity of agonists has gained increasing interest in G protein-coupled receptor (GPCR) research, e.g. due to expectations of drugs with reduced adverse effects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.023DOI Listing

Establishment of an HPLC-based method to identify key proteases of proteins in vitro.

Anal Biochem 2019 May 5;573:1-7. Epub 2019 Mar 5.

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Given that the biological functions of proteins may decrease or even be lost due to degradation by proteases, it is of great significance to identify potential proteases that degrade protein drugs during systemic circulation. In this work, we describe a method based on high-performance liquid chromatography (HPLC) to identify key proteases that degrade therapeutic proteins in blood, including endopeptidases and exopeptidases. Here, the degradation of proteins was detected by competition with standard substrates of proteases and is shown as the relative residue rate. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00032697193002
Publisher Site
http://dx.doi.org/10.1016/j.ab.2019.02.030DOI Listing
May 2019
4 Reads

AFFINImeter: A software to analyze molecular recognition processes from experimental data.

Anal Biochem 2019 Mar 5. Epub 2019 Mar 5.

Fundación Pública Galega Centro Tecnolóxico de Supercomputación de Galicia (CESGA), Avda. de Vigo s/n, 15705, Santiago de Compostela, Spain.

The comprehension of molecular recognition phenomena demands the understanding of the energetic and kinetic processes involved. General equations valid for the thermodynamic analysis of any observable that is assessed as a function of the concentration of the involved compounds are described, together with their implementation in the AFFINImeter software. Here, a maximum of three different molecular species that can interact with each other to form an enormous variety of surpramolecular complexes are considered. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.031DOI Listing
March 2019
1 Read

A reanalysis of protein tyrosine phosphatases inhibitory studies using the unnatural substrate analogue p-nitrophenyl phosphate.

Authors:
Ryan Walsh

Anal Biochem 2019 May 4;572:58-62. Epub 2019 Mar 4.

Department of Microbiology and Biochemistry, INRS-Institut Armand-Frappier, Laval, Québec, H7V 1B7, Canada. Electronic address:

The determination of inhibition mode is extremely important in the understanding of drug interactions and biological mechanisms. The data presented by Hjortness et al. in their recent papers [1,2] on the inhibition of various Protein Tyrosine Phosphatases addresses this issue in an exemplary manner, determining the mode of inhibition based on global fitting of the data to multiple models of inhibition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.025DOI Listing

Development and evaluation of a C-ELISA for rapid detection of antibody to AIV- H7.

Anal Biochem 2019 May 4;572:52-57. Epub 2019 Mar 4.

Wuhan Keqian Biological Co., Ltd., Wuhan, China. Electronic address:

Since 2013, the H7 subtype avian influenza virus (AIV-H7) has seriously endangered human life and health, and has had a serious impact on the poultry industry in China. A competitive enzyme-linked immunosorbent assay (C-ELISA) which detects the antibody for AIV-H7 was developed, basing on a monoclonal antibody (mAb) against the neutralizing epitopes on hemagglutinin (HA)gene. Twelve hybridoma cell lines were screened by cell fusion. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.024DOI Listing
May 2019
2 Reads

Synthesis of titanium dioxide nanotubes with liposomal covers for carrying and extended release of 5-FU as anticancer drug in the treatment of HeLa cells.

Anal Biochem 2019 May 2;572:16-24. Epub 2019 Mar 2.

Quality Control Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Nano-titanium dioxide (nano-TiO) is an important material used in commerce today. In this study, titanium dioxide nanotubes (TNTs) were synthesized through the electrochemical anodizing method. Subsequently, 5-fluorouracil (5-FU), an anticancer drug, was loaded into the nanotubes by the drop-wise method. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.027DOI Listing
May 2019
5 Reads

Cloning of a novel trypsin inhibitor from the Traditional Chinese medicine decoction pieces, Radix Trichosanthis.

Anal Biochem 2019 Mar 1. Epub 2019 Mar 1.

Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast, Northern Ireland, UK.

Most herbs of traditional Chinese medicine (TCM) are used as air-dried decoction pieces that are manufactured and kept at ambient temperature for long periods. Given the ability of some desiccation-tolerant plants to conserve RNA, it could be worthwhile to isolate mRNA from TCM decoction pieces as part of a transcriptomic strategy to identify new substances with potential pharmaceutical application. Here, we report the molecular cloning of a novel trypsin inhibitor (as the probable alleleic variants TKTI-2 and TKTI-3) from the decoction piece of Radix Trichosanthis, representing the dried root of Trichosanthes kirilowii. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.028DOI Listing
March 2019
2 Reads

Preparation and identification of chicken egg yolk immunoglobulins against human enterovirus 71 for diagnosis of hand-foot-and-mouth disease.

Anal Biochem 2019 May 1;573:44-50. Epub 2019 Mar 1.

School of Public Health, Jilin University, Changchun, Jilin, China.

Human enterovirus 71 (EV71) is one of the major pathogens that causes hand-foot-and mouth disease, and there is an urgent need for rapid diagnosis of EV71 virus infection for early antiviral treatment. The aim of this study was to prepare chicken egg yolk immunoglobulin Y (IgY) for the diagnosis of enterovirus type 71 infection. The antibodies were raised by intramuscular immunization of laying hens with inactivated human EV71 and isolated from the egg yolk by multiple steps of polyethylene glycol 6000 extraction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.029DOI Listing
May 2019
3 Reads

Glycation profile of minor abundant erythrocyte proteome across varying glycemic index in diabetes mellitus.

Anal Biochem 2019 May 2;573:37-43. Epub 2019 Mar 2.

Clinical Proteomics Unit, Division of Molecular Medicine, St. John's Research Institute, St. John's National Academy of Health Sciences, 100ft Road, Koramangala, Bangalore, 560034, India. Electronic address:

Purpose: Long-term glycemic index in patients with diabetes mellitus (DM) is measured by glycated hemoglobin (HbA) besides blood glucose. In DM, the primary amino groups of proteins get glycated via non-enzymatic post-translational modification. This study aims at identifying and characterizing site-specific glycation of erythrocyte proteome across varying glycemic index in patients with DM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.026DOI Listing
May 2019
2.219 Impact Factor

One-step immunoassay for the insecticide carbaryl using a chicken single-chain variable fragment (scFv) fused to alkaline phosphatase.

Anal Biochem 2019 May 1;572:9-15. Epub 2019 Mar 1.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China. Electronic address:

Immunoassays provide a high-throughput method for monitoring pesticides in foods and the environment. Due to easy generation and capable of being manipulated, chicken single-chain variable fragment (scFv) is attractive in the development of immunoassays for pesticides. Two scFvs (X1 and X2) against the insecticide carbaryl were generated from a chicken immunized with hapten C1 conjugated to keyhole limpet hemocyanin and fused with alkaline phosphatase (AP) to develop a rapid one-step enzyme-linked immunosorbent assay for this pesticide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.022DOI Listing
May 2019
3 Reads

Pulsed Field Gel Electrophoresis: Past, present, and future.

Anal Biochem 2019 May 28;573:17-29. Epub 2019 Feb 28.

Academy of Biology, School of Sciences and Humanities, UACM, Mexico City, Mexico. Electronic address:

Pulsed Field Gel Electrophoresis (PFGE) has been considered for many years the 'gold-standard' for characterizing many pathogenic organisms as well as for subtyping bacterial species causing infection outbreaks. This article reviews the basic principles of PFGE and it includes the main advantages and limitations of the different electrode configurations that have been used in PFGE equipment and their influence on the DNA electrophoretic separation. Remarkably, we summarize here the most relevant theoretical and practical aspects that we have learned for more than 20 years developing and using the miniaturized PFGE systems. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.020DOI Listing

Extraction methods and computational approaches for evaluation of antimicrobial compounds from Capparis zeylanica L.

Anal Biochem 2019 May 27;572:33-44. Epub 2019 Feb 27.

Computational Drug Design Lab, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India.

Capparis zeylanica Linn (Caparadaceae), a well-known traditional medicinal plant has been used prevalently in the Ayurvedic system of medicine. It has long been used in treating cholera, hemiplegia, pneumonia, helmintic and inflammatory activity. This study aims to investigate the antimicrobial activity of C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.006DOI Listing
May 2019
7 Reads

High-throughput amenable fluorescence-assays to screen for calmodulin-inhibitors.

Anal Biochem 2019 May 27;572:25-32. Epub 2019 Feb 27.

Cancer Cell Biology and Drug Discovery Group, Life Sciences Research Unit, University of Luxembourg, 4362, Esch-sur-Alzette, Luxembourg; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20520, Turku, Finland. Electronic address:

The KRAS gene is highly mutated in human cancers and the focus of current Ras drug development efforts. Recently the interface between the C-terminus of K-Ras and calmodulin (CaM) was proposed as a target site to block K-Ras driven cancer cell stemness. We therefore aimed at developing a high-throughput amenable screening assay to identify novel CaM-inhibitors as potential K-Ras stemness-signaling disruptors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.015DOI Listing

iEnhancer-5Step: Identifying enhancers using hidden information of DNA sequences via Chou's 5-step rule and word embedding.

Anal Biochem 2019 Apr 26;571:53-61. Epub 2019 Feb 26.

Medical Humanities Research Cluster, School of Humanities, Nanyang Technological University, 48 Nanyang Ave, 639798, Singapore. Electronic address:

An enhancer is a short (50-1500bp) region of DNA that plays an important role in gene expression and the production of RNA and proteins. Genetic variation in enhancers has been linked to many human diseases, such as cancer, disorder or inflammatory bowel disease. Due to the importance of enhancers in genomics, the classification of enhancers has become a popular area of research in computational biology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.017DOI Listing
April 2019
2 Reads
2.219 Impact Factor

Assessment of NADmetabolism in human cell cultures, erythrocytes, cerebrospinal fluid and primate skeletal muscle.

Anal Biochem 2019 May 27;572:1-8. Epub 2019 Feb 27.

Biomedical Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA. Electronic address:

The reduction-oxidation state of NAD/NADH is critical for cellular health with NAD and its metabolites playing critical roles in aging and pathologies. Given the inherent autooxidation of reduced dinucleotides (i.e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2019.02.019DOI Listing
May 2019
1 Read