1,578 results match your criteria Analytical and quantitative cytology and histology[Journal]


Micronuclei and other nuclear anomalies in buccal mucosa following exposure to X-ray radiation.

Anal Quant Cytol Histol 2012 Jun;34(3):161-9

Department of Oral and Maxillofacial Pathology, Annoor Dental College, Kerala, India.

Objective: To evaluate DNA damage and cellular death in exfoliated buccal mucosa cells from healthy individuals following dental X-ray exposure, to compare effects of different types of radiographic techniques using the buccal cytome assay and to standardize the staining procedure.

Study Design: This study comprised 90 patients, categorized into 3 groups subjected to intraoral periapical radiography, conventional orthopantomogram and digital orthopantomogram X-ray exposure. Exfoliated oral mucosa cells were collected immediately before the X-ray and 10 days later and stained using DNA-specific stains and nonspecific DNA stains. Read More

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June 2012
2 Reads

Color deconvolution. Optimizing handling of 3D unitary optical density vectors with polar coordinates.

Authors:
Gilbert Bigras

Anal Quant Cytol Histol 2012 Jun;34(3):149-60

Laboratory Medicine, Cross Cancer Institute, University of Alberta, 11560 University Avenue, Edmonton, Alberta T6G 1Z2, Canada.

Objective: Color deconvolution relies on determination of unitary optical density vectors (OD(3D)) derived from pure constituent stains initially defined as intensity vectors in RGB space. OD(3D) can be defined in polar coordinates (phi, theta, radius); always being equal to one, radius can be ignored. Easier handling of unitary optical density 2D vectors (OD(2D)) is shown. Read More

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June 2012
45 Reads

Nuclear morphometry in the differential diagnosis of eosinophilic renal epithelial tumors.

Anal Quant Cytol Histol 2012 Jun;34(3):145-8

Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey.

Objective: To assess the potential contribution of nuclear morphometry to the differential diagnosis of renal epithelial tumors with eosinophilic cytoplasm, including chromophobe renal cell carcinoma (ChRCC), the eosinophilic variant of clear cell renal cell carcinoma (EoRCC), and oncocytoma.

Study Design: A total of 24 tumor tissue samples diagnosed as ChRCC, the EoRCC, or oncocytoma constituted our series. Eight geometric features such as nuclear area, nuclear perimeter, and circular form factor were measured and compared among the groups. Read More

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June 2012
9 Reads

Spectrum of prostatic nonepithelial tumors and tumorlike conditions with spindle cell features.

Anal Quant Cytol Histol 2012 Jun;34(3):139-44

Section of Pathological Anatomy, Polytechnic University of the Marche Region, School of Medicine, United Hospitals, Ancona, Italy.

There exists a wide and heterogeneous spectrum of spindle cell lesions that can originate from the prostatic stroma. This includes benign and malignant neoplasms as well as tumorlike conditions. Their diagnosis in small biopsy specimens is often difficult because of the potential for sampling error. Read More

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A framework for quantitative assessment of Ki67 distribution in preneoplastic bronchial epithelial lesions.

Anal Quant Cytol Histol 2012 Jun;34(3):120-38

Department of Cancer Imaging, British Columbia Cancer Research Center, Vancouver, British Columbia, Canada.

Objective: Deregulated cell proliferation is a hallmark of cancer, and Ki67 immunostaining can be used to identify proliferating cells. Evaluation of cell proliferation may have utility as a biomarker of epithelial malignant transformation risk. To date, most analyses of Ki67 staining have been restricted to semiquantitative estimations of the degree of staining or the measurement of the fraction of Ki67-positive cells within the epithelium. Read More

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June 2012
3 Reads

Classification and grading of noninvasive and invasive neoplasms of the urothelium.

Anal Quant Cytol Histol 2012 Jun;34(3):111-9

Section of Pathological Anatomy, Polytechnic University of the Marche Region, School of Medicine, United Hospitals, Ancona, Italy.

The classification and grading of the noninvasive, intraepithelial neoplasms of the urothelium are based on the morphological pattern of growth, i.e., papillary and flat (and endophytic), and on their degree of architectural and cytologic abnormalities. Read More

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From biopsy to active surveillance in small renal masses: is it feasible?

Anal Quant Cytol Histol 2012 Apr;34(2):109-10

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April 2012
4 Reads

Multifractal spectrum differentiation of well-differentiated adenocarcinoma from complex atypical hyperplasia of the uterus.

Anal Quant Cytol Histol 2012 Apr;34(2):105-8

Department of Cytopathology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Objective: To introduce a new field of multifractal spectrum in distinguishing between endometrial well-differentiated adenocarcinoma (WDAC) and complex atypical hyperplasia (CAH).

Study Design: Thirteen cases of CAH and 16 of WDAC were selected from radical hysterectomy specimens, and multifractal spectrum was measured from at least 4-5 representative digitized images of each case. The data were collected from f(alpha) vs. Read More

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April 2012
9 Reads

Prostate cancer gene 3 urine assay cutoff in diagnosis of prostate cancer: a validation study on an Italian patient population undergoing first and repeat biopsy.

Anal Quant Cytol Histol 2012 Apr;34(2):96-104

Department of Pathology, San Luigi Gonzaga Hospital and University of Turin, Orbassano.

Objective: To determine an optimal prostate cancer gene 3 (PCA3) cutoff in predicting prostate cancer in Italian patients undergoing first or repeat biopsy.

Study Design: In this observational multicenter study 1246 men with elevated prostate specific antigen (PSA) and negative digital rectal examination, with prostate biopsy after PCA3 assessment, were divided into two groups submitted to PCA3 testing before or after previous negative biopsies. Ideal PCA3 cutoff was identified using area under the curve of the receiver operating characteristic analysis. Read More

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April 2012
2 Reads

Differential diagnosis of follicular tumor by expert systems based on a set of quantitative features of thyrocyte nuclei and aggregates.

Anal Quant Cytol Histol 2012 Apr;34(2):86-95

Laboratory for Electron Paramagnetic Resonance Dosimetry and Cytology, Belarusian State Medical University, Minsk, Belarus.

Objective: To develop expert systems for classification of follicular thyroid tumor at a preoperative stage.

Study Design: Fine needle aspiration biopsy of the thyroid gland with a histologic conclusion of follicular cancer and follicular adenoma were the object of the morphometric study. General sample size was 4500 nuclei and 3000 aggregates. Read More

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April 2012
1 Read

Osteopontin expression in chronic plaque psoriasis: an association with the severity of disease.

Anal Quant Cytol Histol 2012 Apr;34(2):79-85

Department of Pathology, Faculty of Medicine, Menofiya University, Shebein Elkom, Egypt.

Objective: To explore the possible role of osteopontin (OPN) in chronic plaque psoriasis and understand the role of inflammation in psoriasis.

Study Design: We investigated 36 patients with chronic plaque psoriasis using immunohistochemical technique for evaluation of OPN immunolocalization compared to normal skin biopsies of 10 normal subjects representing the control group.

Results: OPN was expressed in the epidermis of all specimens, both in the psoriasis group and the control group without any significant differences except for the tendency of psoriatic lesions to show more cytoplasmic and nuclear pattern of OPN staining (55. Read More

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April 2012
15 Reads

Maspin expression is a favorable prognostic factor in non-small cell lung cancer.

Anal Quant Cytol Histol 2012 Apr;34(2):72-8

Department of Medical Oncology, Section of Pathological Anatomy and Histopathology, Polytechnic University of the Marche Region, Azienda Ospedaliero-Universitaria, Ospedali Riuniti Umberto I-GM Lancisi-G Salesi, Ancona, Italy.

Objective: To evaluate prognostic impact of maspin expression in patients with resected non-small cell lung cancer (NSCLC).

Study Design: From 1996 to 2001, 439 patients underwent radical surgery for NSCLC at the Polytechnic University of the Marche Region. Maspin expression was detected as cytoplasmic and nuclear staining of neoplastic cells. Read More

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April 2012
3 Reads

Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs.

Anal Quant Cytol Histol 2012 Apr;34(2):66-71

Laboratory of Radiobiology, Center for Radiation Protection and Hygiene, and Office for Environmental Regulation and Nuclear Safety, Havana, Cuba.

Objective: To test a CellProfiler pipeline for automated counting and characterization of gamma-H2AX foci in color images of human cultured cells.

Study Design: A431 cells were irradiated and stained for gamma-H2AX foci detection. Sets of color images were analyzed visually, and findings were compared with those using FociCounter and CellProfiler software. Read More

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April 2012
89 Reads

Cell proliferation and apoptosis in prostate needle biopsies with adenocarcinoma Gleason score 6 or 7.

Anal Quant Cytol Histol 2012 Apr;34(2):61-5

Unit of Anatomic Pathology, Department of Surgery, Faculty of Medicine, Cordoba University Medical School, Cordoba, Spain.

Objective: To analyze whether cell proliferation and apoptotic indexes in needle biopsies with prostate cancer Gleason 6 or 7 can identify more objectively Gleason score 6 or 7 in needle biopsy samples.

Study Design: Seventy patients diagnosed by needle biopsy and treated by radical prostatectomy were included. Fifty cases were Gleason score 6 and 20 were Gleason 7. Read More

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Epigenetic therapy in cancer: perspective and paradoxes.

Anal Quant Cytol Histol 2011 Oct;33(5):303-4

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October 2011
3 Reads

Nasopharyngeal nonkeratinizing (undifferentiated) monstrous cell carcinoma. A case report.

Anal Quant Cytol Histol 2011 Oct;33(5):297-302

Atatürk Research and Education Hospital, Izmir, Turkey.

Background: The histologic type of nasopharyngeal carcinoma can be an important factor in the prognosis. The effects, if any, of the degree of cellular pleomorphism is uncertain.

Case: In this report, we present a case of nonkeratinizing, undifferentiated nasopharyngeal carcinoma with monstrous tumor giant cells. Read More

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October 2011
6 Reads

Simple flow cytometric protocol of CD4+/CD8+ lymphocyte ratio assessment in bronchoalveolar lavage fluids from patients with interstitial lung diseases.

Anal Quant Cytol Histol 2011 Oct;33(5):289-96

Laboratory of Molecular Diagnostics and Immunology and Department of Pathomorphology, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland.

Objective: To validate the fast and accurate flow cytometric (FCM) protocol using blood-standardized antibodies for alveolar lymphocyte subtyping with respect to standard immunocytochemistry (IC).

Study Design: FCM and IC were applied to immunophenotype T cell subsets in bronchoalveolar lavage (BAL) fluids from patients with interstitial lung diseases. Diagnostic BAL specimens from 50 patients with suspected sarcoidosis, idiopathic pulmonary fibrosis, and hypersensitivity pneumonitis were evaluated by both IC and FCM. Read More

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October 2011
50 Reads

Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.

Anal Quant Cytol Histol 2011 Oct;33(5):283-8

Department of Pathology, Hospital Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.

Objective: To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.

Study Design: The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Read More

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October 2011
1 Read

Expression of MUC1 (Ma695) in noninvasive papillary urothelial neoplasm according to the 2004 World Health Organization classification of the noninvasive urothelial neoplasm. An immunologic tool for the pathologist?

Anal Quant Cytol Histol 2011 Oct;33(5):277-82

Department of Biopathology, Institut Jean-Godinot, Centre Régional de Lutte Contre le Cancer, Reims Cedex, France.

Objective: To examine the association between MUC1 (Ma695) and noninvasive papillary urothelial neoplasm according to the 2004 World Health Organization classification.

Study Design: Histologic evaluation was performed in 46 patients with nontumoral benign bladder urothelium (n = 11), papillary urothelial neoplasm of low malignant potential (PUNLMP) (n = 14), low-grade papillary urothelial carcinoma (LgPUC) (n = 11), and noninvasive high-grade papillary urothelial carcinoma (HgPUC) (n = 10). Classical morphologic description and semiquantitative digital measurement were performed from immunohistochemical-stained slides using an anti-MUC1 antibody (Ma695). Read More

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October 2011
3 Reads

Cytogenetic abnormalities in exfoliated oral mucosal cells and their association with oral cancer.

Anal Quant Cytol Histol 2011 Oct;33(5):271-6

Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, RS, Brazil.

Objective: To assess cytogenetic abnormalities through quantification of micronuclei, broken eggs, and karyorrhexis, in cells from normal oral mucosa of individuals exposed to carcinogens (alcohol and tobacco) and adjacent to leukoplakias and squamous cell carcinomas.

Study Design: The sample was composed of 40 subjects aged > 30 years, divided into four groups: control, alcohol/tobacco, leukoplakia, and squamous cell carcinoma. For control and alcohol/tobacco groups, cells were collected from lower lip, tongue border, and floor of the mouth. Read More

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October 2011

Discrimination between benign and malignant prostate biopsies using three-dimensional chromatin texture analysis by confocal laser scanning microscopy.

Anal Quant Cytol Histol 2011 Oct;33(5):265-70

Department of Pathology, University Medical Center Utrecht, P.O. Box 85500, 3508 GA Utrecht, The Netherlands.

Objective: To evaluate the clinical usefulness of computing three-dimensional (3-D) nuclear texture features on prostate biopsy specimens to discriminate among benign, prostatic intraepithelial neoplasia (PIN), and malignant specimens.

Study Design: Twelve prostate cancer biopsy specimens were selected, diagnosed as either benign (N = 4), PIN (N = 4), or malignant (N = 4). Sections 14 microm thick were stained. Read More

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October 2011

Differential diagnosis of follicular cancer and follicular adenoma using an expert system based on a set of qualitative signs of cell atypia.

Anal Quant Cytol Histol 2011 Oct;33(5):253-64

Laboratory for Electron Paramagnetic Resonance Dosimetry and Cytology, Belarusian State Medical University, Dzerzhinsky Avenue, 83, 220116 Minsk, Belarus.

Objective: To determine the efficiency of the developed expert system based on a set of qualitative signs of cell atypia and their weighting coefficients in the differential diagnosis of follicular cancer and follicular adenoma of the thyroid gland.

Study Design: Sixty cytologic preparations of patients with histologic diagnosis of a follicular cancer and follicular adenoma were investigated.

Results: Weighting coefficients for each sign of atypia for both forms of pathology have been calculated with the help of the deduced equation. Read More

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October 2011
1 Read

Quantitative microscopy in murine models of lung inflammation.

Anal Quant Cytol Histol 2011 Oct;33(5):245-52

Hawken School, Gates Mills Ohio, USA.

Objective: To develop a quantitative means to measure lung inflammation using the murine models of chronic asthma and cystic fibrosis (CF).

Study Design: Translational-based medicine often utilizes animal models to study new and innovative therapeutics. In asthma and CF, the animal models focus on airway inflammation and remodeling. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195243PMC
October 2011
1 Read

Micronucleus scoring in urine cytology specimen by DNA-specific stain.

Anal Quant Cytol Histol 2012 Feb;34(1):56-60

Department of Cytopathology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Objective: To evaluate the role of micronuclei (MN) scoring in urinary cytology of bladder transitional cell carcinoma (TCC).

Study Design: Eighty cases of freshly voided urine samples were used for scoring of MN using acridine orange (AO)-stained smears under fluorescence microscope and routine Papanicolaou-stained smears under light microscopy. MN score for cytologic lesions was compared. Read More

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February 2012
5 Reads
1 Citation
0.490 Impact Factor

Analysis of performance characteristics of five cell cycle-related immunohistochemical markers and human papillomavirus genotyping in the diagnosis of cervical squamous cell carcinoma precursor lesions.

Anal Quant Cytol Histol 2012 Feb;34(1):49-55

Molecular Pathology and Pharmacogenetics Group, Department of Internal Medicine, Santa María del Rosell University Hospital, Cartagena, Spain.

Objective: To study the performance values of a set of five immunohistochemical markers involved in cell cycle regulation as a potential aid in the differential diagnosis between squamous intraepithelial lesions (SILs) and normal or benign conditions of the uterine cervix.

Study Design: Results from immunohistochemical evaluation of p16, cyclin D1, p53, Ki67, and ProEx C markers and human papillomavirus genotyping were collected from a previous study performed on 37 normal or benign cervices, 39 low grade SILs and 73 high grade SILs. A multivariate analysis was used to examine the specific diagnostic value of each marker and to ascertain those most relevant for SIL diagnosis. Read More

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February 2012
2 Reads

Hormone receptors expression in phyllodes tumors of the breast.

Anal Quant Cytol Histol 2012 Feb;34(1):41-8

Department of Pathology, Chonnam National University Medical School and Research Institute of Medical Sciences, Gwangju, Republic of Korea.

Objective: To ascertain the hormonal receptor profiles of the epithelial and stromal components of phyllodes tumors (PTs) and determine their relationship with stromal proliferation.

Study Design: Eighty-two PTs (50 benign, 22 borderline, and 10 malignant) were studied. Automated immunohistochemical staining for estrogen receptor (ER)-alpha and -beta, progesterone receptor (PR), androgen receptor (AR), and Ki-67 was performed using tissue microarray blocks, and their expression was assessed in both the stromal and epithelial components. Read More

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February 2012
6 Reads

Aberrant connexin 43 and 26 expression in cervical dysplasia.

Anal Quant Cytol Histol 2012 Feb;34(1):28-40

Section of Surgical Pathology, Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, 3400 Spruce Street, Philadelphia, Pennsylvania 19101, USA.

Objective: To determine whether connexin (Cx) expression is altered in cervical dysplasia.

Study Design: Cx proteins form gap junctions and are expressed in squamous epithelia including ectocervix. We used multispectral imaging to perform a quantitative immunohistochemical survey of Cx43 Cx26 in 37 archival human cervical specimens. Read More

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February 2012
8 Reads

Evaluation of peritoneal fluid hemosiderin-laden macrophages in biopsy-proven endometriosis.

Anal Quant Cytol Histol 2012 Feb;34(1):23-7

Reproductive Research Center, Minimally Invasive Surgery Center, Department of Obstetrics-Gynecology, Cleveland Clinic, 9500 Euclid Avenue, A-81, Cleveland, Ohio 44195, USA.

Objective: To evaluate peritoneal fluid hemosiderin-laden macrophages (H-LMs) in patients with endometriosis compared to controls.

Study Design: Consecutive series of 46 patients during a year undergoing laparoscopy for benign gynecologic conditions were included. The presence of H-LMs in peritoneal fluid was evaluated. Read More

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February 2012
11 Reads

Angiogenetic pathway as a therapeutic target in renal cell carcinoma.

Anal Quant Cytol Histol 2012 Feb;34(1):15-22

Medical Oncology Clinic, AOU Ospedali Riuniti, Polytechnic University of the Marche Region, Ancona, Italy.

For several decades, metastatic renal cell carcinoma (mRCC) was considered resistant to therapy and thus linked to poor prognosis. Recent years have seen a complete revolution of the therapeutic landscape, with the introduction of new target therapies that have radically changed treatment strategies in this setting. These new agents have proved to be effective if directed mainly against the angiogenic pathway. Read More

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February 2012
4 Reads

Comparative study of oral mucosa micronuclei in smokers and alcoholic smokers.

Anal Quant Cytol Histol 2012 Feb;34(1):9-14

Department of Biosciences and Oral Diagnosis,, São José dos Campos Dental School, São Paulo State University, São José dos Campos, São Paulo, Brazil.

Objective: To compare the frequency of micronuclei and metanucleated anomalies in the oral mucosa of smokers, alcoholic smokers, and nonsmokers.

Study Design: Three groups were studied: group I, 15 smokers; group II, 16 alcoholic smokers; and group III, 20 nonsmokers. Three smears from the lateral left border of the tongue were processed for Feulgen staining. Read More

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February 2012
5 Reads

Karyometry of nuclear phenotypes in cutaneous squamous cell cancer.

Anal Quant Cytol Histol 2012 Feb;34(1):1-8

College of Optical Sciences, Arizona Cancer Center, University of Arizona, 1515 North Campbell Avenue, P.O. Box 245024, Tucson, Arizona 85724-5024, USA.

Objective: To establish the karyometric characteristics of the two main nuclear phenotypes in cutaneous squamous cell cancer (cSCC) lesions.

Study Design: The clinical materials comprised 75 cases of cSCC, 38 with aggressive lesions and 37 with nonaggressive lesions. High-resolution images of 100 nuclei per case were recorded. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074412PMC
February 2012
12 Reads

Pelvic urothelial carcinoma with nested pattern of growth and an uncommon clinical presentation: a case report.

Anal Quant Cytol Histol 2011 Dec;33(6):340-4

Section of Anatomic Pathology, Department of Human Pathology and Oncology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.

Background: Nested variant of urothelial carcinoma (NVUC) is a rare and often unrecognized urothelial neoplasia. Diagnosis is based on morphology only, and no immunohistochemical or cytogenetic differences from usual high-grade urothelial carcinomas have been reported.

Case: We describe the case of a 49-year-old woman affected by hepatitis C virus presented with fever, discomfort, urgency, and hypertension. Read More

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December 2011
3 Reads

Application of an artificial neural network in the prognosis of chronic myeloid leukemia.

Anal Quant Cytol Histol 2011 Dec;33(6):335-9

Department of Cytology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.

Objective: To use a commercially available artificial neural network (ANN) software program to distinguish prognostically good and bad cases of chronic myeloid leukemia (CML).

Study Design: A total of 40 patients with CML who developed blast crisis or proceeded in the accelerated phase were selected. They formed two groups, group 1 and group 2, of 20 patients each, who developed accelerated phase or blast crisis within 18 months or 30 months, respectively, after the initial diagnosis of the chronic phase of CML. Read More

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December 2011
6 Reads

Cascaded learning vector quantizer neural networks for the discrimination of thyroid lesions.

Anal Quant Cytol Histol 2011 Dec;33(6):323-34

Department of Surgery, Attikon University Hospital, University of Athens Medical School, Athens, Greece.

Objective: To investigate capability of combination of learning vector quantizer (LVQ) neural networks (NNs) in discrimination of benign from malignant thyroid lesions.

Study Design: The study included 335 liquid-based cytology, fine needle aspiration (FNA), Papanicolaou-stained specimens. All cases were compared to the histologic diagnosis. Read More

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December 2011
26 Reads

Molecular findings of nasopharyngeal carcinoma in a European population.

Anal Quant Cytol Histol 2011 Dec;33(6):316-22

Anatomy and Pathology Service, Infanta Margarita Hospital, Cabra, Spain.

Objective: To explore biomolecular characteristics of a group of patients with nasopharyngeal carcinoma from European (Spanish) hospitals, addressing the pathogenesis of the tumor and the response to treatment.

Study Design: Cyclin D1 and p16 expression were evaluated immunohistochemically in 33 tissue samples of nasopharyngeal carcinoma. CCDN1 gene amplification and p16 gene deletion were studied by fluorescence in situ hybridization. Read More

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December 2011
2 Reads

Human papillomavirus E6/E7 mRNA testing has higher specificity than liquid-based DNA testing in the evaluation of cervical intraepithelial neoplasia.

Anal Quant Cytol Histol 2011 Dec;33(6):311-5

Institute of Pathology, University of Witten/Herdecke, Wuppertal, Germany.

Objective: To examine the specificity of human papillomavirus (HPV) E6/E7 mRNA testing for intraepithelial precursor lesions and invasive carcinoma of the uterine cervix in 358 women and compare the results with those of the most widely used DNA technique.

Study Design: For HPV E6/E7 mRNA testing an amplification assay was used. For DNA determination a hybridization assay was applied. Read More

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December 2011
1 Read

Clinical performance of human papillomavirus E6, E7 mRNA flow cytometric assay compared to human papillomavirus DNA typing.

Anal Quant Cytol Histol 2011 Dec;33(6):305-10

Department of Cytopathology, Attikon University Hospital, Athens University Medical School, 1 Rimini Street, 12462 Chaidari, Athens, Greece.

Objective: To use flow cytometry to screen cervical samples for the overexpression of human papillomavirus (HPV) E6 and E7 mRNA and compare the performance of this assay with an HPV DNA array for the detection of high-grade cervical lesions.

Study Design: Cervical samples were analyzed for HPV DNA by clinical arrays, and the overexpression of E6 and E7 viral oncogenes was monitored using an HPV mRNA detection kit that quantifies the intracellular HPV E6 and E7 mRNA on a cell-by-cell basis.

Results: HPV positivity increased with severity of histologic lesions. Read More

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December 2011
20 Reads

Upregulation of AgNOR expression in epithelial cells and neutrophils in the airways and leukocytes in peripheral blood of women chronically exposed to biomass smoke.

Anal Quant Cytol Histol 2011 Feb;33(1):50-9

Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata, India.

Objective: To evaluate the impact of indoor air pollution from biomass fuel use on ribosome biogenesis in airway cells and peripheral blood leukocytes using the argyrophilic nucleolar organizer region (AgNOR) staining technique.

Study Design: Biomass users were represented by 78 never-smoking, premenopausal women from rural India and a control group of 73 age-matched women who cooked with liquefied petroleum gas (LPG). For silver staining, exfoliated airway cells and circulating lymphocytes and neutrophils were obtained from expectorated sputum and venous blood smears, respectively. Read More

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February 2011
5 Reads

Validation of a histologic scoring system for the examination of quality of tendon graft to bone tunnel healing in anterior cruciate ligament reconstruction.

Anal Quant Cytol Histol 2011 Feb;33(1):36-49

Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T.

Objective: To develop and validate a histologic scoring system for the assessment of tendon graft to bone tunnel healing in anterior cruciate ligament (ACL) reconstruction.

Study Design: The scoring system, tendon-bone tunnel healing (TBTH) score, comprised 5 items on graft status,fiber type and interface connectivity, evaluated on either a 5- or 6-point scale. Two observers were trained to use the scoring system, examining 15 blinded histologic slides from an ongoing study. Read More

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February 2011
9 Reads

Cell cycle analysis of colorectal brushings collected in liquid-based cytology.

Anal Quant Cytol Histol 2011 Feb;33(1):29-35

Department of Cytopathology, University General Hospital Attikon, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.

Objective: To evaluate the possibility of robust cell cycle analysis from liquid-based cytology samples.

Study Design: Brushings were obtained after surgical resection of tumor samples. For each patient, one brushing sample was obtained from the macroscopically identified tumor, and a matched sample from a distant normal-appearing site. Read More

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February 2011
10 Reads

Automated screening of gynecologic cytology: a comparison of results.

Anal Quant Cytol Histol 2011 Feb;33(1):25-8

Cytopathology Unit, Department of Pathology, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain.

Objective: To compare the results among conventional gynecologic cytology liquid-based cytology and manual screening and liquid-based cytology automatically screened.

Study Design: The results of 53,311 cervicovaginal samples, corresponding to 53,311 women of Barcelona, Spain are reviewed. The cases are subdivided into 19,742 conventional cervicovaginal samples; 17,596 liquid-based cytology, manually screened samples; and 15,973 liquid-based automated-screened samples. Read More

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February 2011
6 Reads

Cytokeratin profile in exfoliative cytology of smokers.

Anal Quant Cytol Histol 2011 Feb;33(1):19-24

Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University, São José dos Campos, São Paulo, Brazil.

Objective: To compare the expression of cytokeratins (CKs) 6, 16, 19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers to determine the proliferative activity and expression indicative of a potential for malignant transformation.

Study Design: Smears were obtained from the left lateral border of the tongue with a cytobrush from 25 smokers and 20 nonsmokers seen at the clinics of São José dos Campos Dental School, São Paulo State University, São José dos Campos, São Paulo, Brazil, and processed for immunohistochemistry. Conventional microscopy was used for qualitative analysis. Read More

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February 2011
4 Reads

Consistency of histomorphometric measurements of normal oral epithelium subjected to variations in routine tissue processing.

Anal Quant Cytol Histol 2011 Feb;33(1):12-8

Department of Oral Pathology, J. K. K. Nataraja Dental College and Hospital, Komarapalayam, Tamil Nadu, and Educare Institute of Dental Sciences, Chattiparamba, Malappuram, Kerala, India.

Objective: To determine the effect of formalin fixation time on histomorphometry by changing the fixation time and the effect of the age of processing solutions on histomorphometry by processing tissues in solutions of varying ages.

Study Design: The study consisted of two parts. The first part examined the effect of formalin fixation time on histomorphometry by changing the fixation time. Read More

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February 2011

Old and new immunohistochemical markers for the diagnosis of gastrointestinal stromal tumors.

Anal Quant Cytol Histol 2011 Feb;33(1):1-11

Anatomic Pathology Service, Virgen-Macarena University Hospital, Faculty of Medicine, Seville, Spain.

Gastrointestinal stromal tumors (GISTs) are generally CD117-positive and KIT or PDGFRA mutation-driven mesenchymal tumors of the gastrointestinal tract, probably originating in interstitial cells of Cajal or related precursors. CD117 is the best diagnostic marker for GISTs, but 5-10% are negative. Staining pattern may be cytoplasmic, membrane, and paranuclear (Golgi pattern). Read More

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February 2011
8 Reads

Coexistence of bladder prostatic-type polyp and urothelial papilloma.

Anal Quant Cytol Histol 2010 Oct;32(5):299-300

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October 2010
6 Reads

Mature cystic teratoma of the appendix: a case report.

Anal Quant Cytol Histol 2010 Oct;32(5):295-8

Department of Pathology, University of Louisville Hospital, 530 South Jackson Street, Room COF 19, Louisville, Kentucky 40202, USA.

Background: Teratomas very rarely arise from the appendix. To our knowledge, only one prior case of mature teratoma involving the appendix has been reported in the medical literature.

Case: Our case is the second reported case of mature cystic teratoma involving the appendix, and, to our knowledge, it is the first reported in a female who had two simultaneous teratomas, one arising from the appendix and one arising from the right ovary. Read More

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October 2010
3 Reads

Mean nuclear volume in complex atypical hyperplasia and adenocarcinoma of the endometrium.

Anal Quant Cytol Histol 2010 Oct;32(5):291-4

Department of Cytology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Objective: To explore the possible role of mean nuclear volume (MNV) in differentiating complex atypical hyperplasia (CAH) from well-differentiated adenocarcinoma (WDAC) of the endometrium.

Study Design: In this retrospective study we measured MNV in 15 cases each of CAH and WDAC using the point intercepts method. At least 100 nuclei were selected for MNV estimation in each case. Read More

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October 2010

Quantificational and statistical analysis of the differences in centrosomal features of untreated lung cancer cells and normal cells.

Anal Quant Cytol Histol 2010 Oct;32(5):280-90

Risk Assessment, Detection and Intervention Program, Cancer Prevention and Control Division, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, MRC-West, Tampa, Florida 33612, USA.

Objective: To distinguish untreated lung cancer cells from normal cells through quantitative analysis and statistical inference of centrosomal features extracted from cell images.

Study Design: Recent research indicates that human cancer cell development is accompanied by centrosomal abnormalities. For quantitative analysis of centrosome abnormalities, high-resolution images of normal and untreated cancer lung cells were acquired. Read More

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October 2010
9 Reads

Effects of image resolution and noise on estimating the fractal dimension of tissue specimens.

Anal Quant Cytol Histol 2010 Oct;32(5):269-79

Department of Oncology, University of Calgary and Tom Baker Cancer Center, 1331-29 Street Northwest, Calgary, Alberta T2N 4N2, Canada.

Objective: To investigate the effects of imaging system noise and resolution on the ability to estimate and distinguish relative differences in the fractal dimension of tissue specimens.

Study Design: Mathematically derived test images of known fractal dimension mimicking the complexity of epithelial morphology were created. The box-counting method was used to compute fractal dimension. Read More

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October 2010
4 Reads

Usefulness and limitations of alpha-methylacyl-CoA racemase expression in the hepatobiliary system and pancreas: a wide tissue microarray analysis of normal and neoplastic epithelia.

Anal Quant Cytol Histol 2010 Oct;32(5):261-8

Department of Anatomic Pathology, Hospital Clinic and August Pi i Sunyer Biomedical Research Institute, University of Barcelona, Barcelona, Spain.

Objective: To establish the frequency, significance and clinical utility of alpha-methylacyl-CoA racemase (AMACR) expression in the hepatobiliary system by performing a wide immunohistochemical screening of AMACR expression.

Study Design: A total of 204 neoplastic, preneoplastic and normal samples from the hepatobiliary and pancreatic system were immunohistochemically studied on tissue microarrays for AMACR expression and correlated with clinicopathologic parameters.

Results: Hepatocellular carcinomas (HCC) showed intense AMACR expression, which was significantly stronger than in nonmalignant tissues and correlated with good differentiation but not with evolution. Read More

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October 2010
2 Reads