13,694 results match your criteria Analytical and bioanalytical chemistry[Journal]


Size-dependent sub-proteome analysis of urinary exosomes.

Anal Bioanal Chem 2019 Feb 14. Epub 2019 Feb 14.

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200438, China.

Exosomes are cell-derived functional microparticles which exist in most body fluids. They carry abundant signaling molecules to transfer information between cells and microenvironment. Research on exosomes' heterogeneity and constitute variations has been a heated topic in recent years. Read More

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http://dx.doi.org/10.1007/s00216-019-01616-5DOI Listing
February 2019

Highly sensitive and selective "off-on" fluorescent sensing platform for ClO in water based on silicon quantum dots coupled with nanosilver.

Anal Bioanal Chem 2019 Feb 14. Epub 2019 Feb 14.

School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

We present a new "off-on" fluorescence probe for detecting hypochlorite (ClO) based on silicon quantum dots coupled with silver nanoparticles (SiQDs/AgNPs) as nanocomplexes. Via introducing N-[3-(trimethoxysilyl)propyl]ethylenediamine and catechol as initial reactants, silicon quantum dots (SiQDs) with excellent properties were synthesized through a simple hydrothermal method. Transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the morphology and structure of quantum dots. Read More

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http://dx.doi.org/10.1007/s00216-019-01597-5DOI Listing
February 2019

Classifying single fibers based on fluorinated surface treatments.

Anal Bioanal Chem 2019 Feb 14. Epub 2019 Feb 14.

Department of Chemistry, Georgetown University, Washington, DC, USA.

Fibers are an important form of forensic evidence, but their evidential value can be severely limited when the identified characteristics of the fibers are common, such as blue cotton. Detecting chemical fiber treatments offers an avenue to further classify fibers and to improve their evidential value. In this report, we investigate the potential of fluoropolymer fiber coatings, used to impart oil and water-repellent properties in fabrics, for differentiating between fibers. Read More

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http://dx.doi.org/10.1007/s00216-019-01596-6DOI Listing
February 2019

Recent trends in analysis of nanoparticles in biological matrices.

Anal Bioanal Chem 2019 Feb 14. Epub 2019 Feb 14.

NRC Metrology, 1200 Montreal Road, Ottawa, ON, K1A0R6, Canada.

The need to assess the human and environmental risks of nanoparticles (NPs) has prompted an adaptation of existing techniques and the development of new ones. Nanoparticle analysis poses a great challenge as the analytical information has to consider both physical (e.g. Read More

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http://dx.doi.org/10.1007/s00216-019-01620-9DOI Listing
February 2019
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Fluorescent kinetics combined with fourth-order calibration for the determination of diclofenac sodium in environmental water.

Anal Bioanal Chem 2019 Feb 13. Epub 2019 Feb 13.

College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.

A method that combines five-way fluorescence kinetics with fourth-order calibration for interference-free quantification of diclofenac sodium in river water was proposed and tested. Traditional fluorescence methods may not be suitable for such measurements since the fluorescence properties of the analyte are highly dependent on both pH and irradiation time in situ. In the method considered here, a five-way emission-excitation-time-pH data array was obtained from the samples by introducing the pH level and irradiation time as two extra modes. Read More

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http://dx.doi.org/10.1007/s00216-019-01624-5DOI Listing
February 2019

A hierarchical cobalt/carbon nanotube hybrid nanocomplex-based ratiometric fluorescent nanosensor for ultrasensitive detection of hydrogen peroxide and glucose in human serum.

Anal Bioanal Chem 2019 Feb 13. Epub 2019 Feb 13.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety (Ministry of Education), College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Highly sensitive detection of HO and glucose is critical for fundamental research and disease diagnosis. A ratiometric sensor can simultaneously afford two readout signals that provide an internally normalized response to change, thereby reducing false results and improving detection accuracy. A novel ratiometric fluorescent nanosensor for ultrasensitive detection of hydrogen peroxide and glucose was constructed on the basis of the peroxidase-like properties of a hierarchical cobalt/carbon nanotube hybrid nanocomplex (Co-CNT). Read More

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http://dx.doi.org/10.1007/s00216-019-01573-zDOI Listing
February 2019

Liposome protein corona characterization as a new approach in nanomedicine.

Anal Bioanal Chem 2019 Feb 13. Epub 2019 Feb 13.

Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy.

This trends article describes the analytical approaches for the in-depth characterization of the protein corona on liposome nanoparticles. In particular, examples since 2014 are summarized according to the analytical approach. Traditional protein corona characterizations from in vitro static experiments are provided along with the newly introduced experimental setups for characterization of the protein corona by in vitro dynamic and in vivo studies. Read More

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http://dx.doi.org/10.1007/s00216-019-01656-xDOI Listing
February 2019

The role of incurred materials in method development and validation to account for food processing effects in food allergen analysis.

Anal Bioanal Chem 2019 Feb 13. Epub 2019 Feb 13.

Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124, Parma, Italy.

The issue of undeclared allergens represents a matter of great concern, being the subject of many alert notifications by the Rapid Alert System for Food and Feed portal of the European Commission, often leading to food recalls. The availability of reliable analytical approaches able to detect and quantify hidden allergens in processed foods is increasingly requested by the food industry, food safety authorities and regulatory bodies to protect sensitive consumers' health. The present review discusses the fundamental role of incurred materials for method development and analytical performance assessment in a metrology perspective on testing for undeclared allergens in processed foodstuffs. Read More

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http://dx.doi.org/10.1007/s00216-019-01642-3DOI Listing
February 2019

Enhanced electrochemiluminescent brightness and stability of porphyrins by supramolecular pinning and pinching for sensitive zinc detection.

Anal Bioanal Chem 2019 Feb 12. Epub 2019 Feb 12.

Key Laboratory of New Membrane Materials, Ministry of Industry and Information Technology School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu, China.

Ultrasensitive electrochemiluminescence (ECL) detection can benefit substantially from the rational configuration of emitter-enhancer stereochemistry. Here, using zinc(II) meso-5,10,15,20-tetra(4-sulfonatophenyl)porphyrin (ZnTSPP) as a model, we demonstrate that both the ECL intensity and the photostability of this emitter were significantly improved when it was trapped in pyridyl-bridged β-cyclodextrin dimer (Py(CD)); a synthetic enhancer that is ECL inactive. Through NMR characterization, we confirmed that ZnTSPP formed a clam-like inclusion complex involving pinning and pinching forces from the biocompatible container Py(CD). Read More

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http://dx.doi.org/10.1007/s00216-019-01634-3DOI Listing
February 2019

Joshua Edel, Aleksandar Ivanov, MinJun Kim (Eds.): Nanofluidics, 2nd ed.

Authors:
Aaron Timperman

Anal Bioanal Chem 2019 Feb 12. Epub 2019 Feb 12.

University of Illinois at Urbana-Champaign, Champaign, IL, USA.

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http://dx.doi.org/10.1007/s00216-019-01585-9DOI Listing
February 2019

Metabolomic and lipidomic characterization of Oxalobacter formigenes strains HC1 and OxWR by UHPLC-HRMS.

Anal Bioanal Chem 2019 Feb 11. Epub 2019 Feb 11.

Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32610, USA.

Diseases of oxalate, such as nephrolithiasis and primary hyperoxaluria, affect a significant portion of the US population and have limited treatment options. Oxalobacter formigenes, an obligate oxalotrophic bacterium in the mammalian intestine, has generated great interest as a potential probiotic or therapeutic treatment for oxalate-related conditions due to its ability to degrade both exogenous (dietary) and endogenous (metabolic) oxalate, lowering the risk of hyperoxaluria/hyperoxalemia. Although all oxalotrophs degrade dietary oxalate, Oxalobacter formigenes is the only species shown to initiate intestinal oxalate secretion to draw upon endogenous, circulating oxalate for consumption. Read More

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http://dx.doi.org/10.1007/s00216-019-01639-yDOI Listing
February 2019
1 Read

Succinylated Jeffamine ED-2003 coated polycarbonate chips for low-cost analytical microarrays.

Anal Bioanal Chem 2019 Feb 11. Epub 2019 Feb 11.

Institute of Hydrochemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Marchioninistrasse 17, 81377, Munich, Germany.

Analytical microarrays feature great capabilities for simultaneous detection and quantification of multiple analytes in a single measurement. In this work, we present a rapid and simple method for bulk preparation of microarrays on polycarbonate sheets. Succinylated Jeffamine® ED-2003 was screen printed on polycarbonate sheets to create a polyfunctional shielding layer by baking at 100 °C. Read More

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http://dx.doi.org/10.1007/s00216-019-01594-8DOI Listing
February 2019
2 Reads

Simultaneous detection of fumonisin B and ochratoxin A using dual-color, time-resolved luminescent nanoparticles (NaYF: Ce, Tb and NH-Eu/DPA@SiO) as labels.

Anal Bioanal Chem 2019 Feb 9. Epub 2019 Feb 9.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.

A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B (FB) was developed using multi-color, Ln-doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF: Ce, Tb and NH-Eu/DPA@SiO NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized FeO magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb and Eu, respectively, were used to measure FB (Y = 19,177.1 + (- 12,054. Read More

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http://dx.doi.org/10.1007/s00216-019-01580-0DOI Listing
February 2019

Graphene-modified electrodes for sensing doxorubicin hydrochloride in human plasma.

Anal Bioanal Chem 2019 Feb 9. Epub 2019 Feb 9.

Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520-IEMN, 59000, Lille, France.

Doxorubicin (DOX), an anthracycline molecule, is currently one of the most widely used anticancer drugs in clinics. Systematic treatment of patients with DOX is known to be accompanied by several unpleasant side effects due to the toxicity of the drug. Thus, monitoring of DOX concentration in serum samples has become increasingly important to avoid side effects and ensure therapeutic efficiency. Read More

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http://dx.doi.org/10.1007/s00216-019-01611-wDOI Listing
February 2019

Quantitative characterization of glutaminolysis in human plasma using liquid chromatography-tandem mass spectrometry.

Anal Bioanal Chem 2019 Feb 9. Epub 2019 Feb 9.

Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Tongjiaxiang No. 24, Nanjing, 210009, Jiangsu, China.

Glutaminolysis is the metabolic pathway that lyses glutamine to glutamate, alanine, citrate, aspartate, and so on. As partially recruiting reaction steps from the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle, glutaminolysis takes essential place in physiological and pathological situations. We herein developed a sensitive, rapid, and reproducible liquid chromatography-tandem mass spectrometry method to determine the perturbation of glutaminolysis in human plasma by quantifying 13 involved metabolites in a single 20-min run. Read More

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http://dx.doi.org/10.1007/s00216-019-01626-3DOI Listing
February 2019
1 Read

Label-free evaluation of small-molecule-protein interaction using magnetic capture and electrochemical detection.

Anal Bioanal Chem 2019 Feb 9. Epub 2019 Feb 9.

Department of Chemistry, Federal University of São Carlos, CEP, São Carlos, SP, 13565-905, Brazil.

The evaluation of interaction between small molecules and protein is an important step in the discovery of new drugs and to study complex biological systems. In this work, an alternative method was presented to evaluate small-molecule-protein interaction by using ligand capture by protein-coated magnetic particles (MPs) and disposable electrochemical cells. The interaction study was conducted using [10]-gingerol from ginger rhizome and a transmembrane protein αVβ3 integrin. Read More

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http://dx.doi.org/10.1007/s00216-019-01636-1DOI Listing
February 2019

Correction to: Analysis of glipizide binding to normal and glycated human serum albumin by high-performance affinity chromatography.

Anal Bioanal Chem 2019 Feb 9. Epub 2019 Feb 9.

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.

The authors would like to call the reader's attention to the following corrections in this article. In the description given for the process of preparing glycated human serum albumin under "In vitro glycation of HSA", the concentrations of D-glucose that were employed were 15 mM and 30 mM. Read More

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http://dx.doi.org/10.1007/s00216-019-01648-xDOI Listing
February 2019

A novel optical approach for determination of prolactin based on Pr-MOF nanofibers.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Department of Inorganic Chemistry, National Research Centre, 33 El-Behouth St., Dokki, Giza, 12622, Egypt.

The analytical quantification and follow-up of the hormone prolactin is very important in clinical diagnosis (e.g., in cases of breast cancer), treatment, and the medical laboratory. Read More

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http://link.springer.com/10.1007/s00216-018-01564-6
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http://dx.doi.org/10.1007/s00216-018-01564-6DOI Listing
February 2019
2 Reads

A high-throughput metabolomics approach for the comprehensive differentiation of four Pulsatilla Adans herbs combined with a nontargeted bidirectional screen for rapid identification of triterpenoid saponins.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, Hebei, China.

Pulsatilla Adans (PSA) herbs (Ranunculaceae) have been widely used in traditional medicine in China and other countries. However, the authentication and quality control of PSA herbs have always been a challenging task due to their similar morphological characteristics and the diversity of the multiple components that exist in the complicated matrix. Herein, a novel integrated strategy combining UHPLC/Q-Orbitrap-MS techniques with chemometrics analysis is proposed for the discrimination of PSA materials. Read More

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http://dx.doi.org/10.1007/s00216-019-01631-6DOI Listing
February 2019
1 Read
3.436 Impact Factor

Determination of the polyphenolic fraction of Pistacia vera L. kernel extracts by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry detection.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata, Viale Annunziata, 98168, Messina, Italy.

Pistachio (Pistacia vera L.) belongs to the Anacardiaceae family and it is a small tree species. It is native of the Middle East and Central Asia, but currently, it is cultivated also in California and in some Mediterranean countries, such as Greece and Italy. Read More

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http://dx.doi.org/10.1007/s00216-019-01649-wDOI Listing
February 2019

Yury A. Zolotov: Russian contributions to analytical chemistry.

Authors:
Reiner Salzer

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Faculty of Chemistry and Food Chemistry, TU Dresden, 01062, Dresden, Germany.

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http://dx.doi.org/10.1007/s00216-019-01619-2DOI Listing
February 2019

DNA-modulated photosensitization: current status and future aspects in biosensing and environmental monitoring.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

College of Chemistry, Sichuan University, Chengdu, 610064, China.

Recently, photosensitized oxidation has been explored in many fields of research and applications, such as photodynamic therapy (PDT) and photodynamic antimicrobial chemotherapy (PACT). Although the photosensitized generation of ROS features emerging applications, controllable management of the photosensitization process is still sometimes problematic. DNA has long been considered the carrier for genetic information. Read More

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http://dx.doi.org/10.1007/s00216-019-01605-8DOI Listing
February 2019

Nanobody: outstanding features for diagnostic and therapeutic applications.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Nanobiotechnology for Diagnostics Group (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034, Barcelona, Spain.

Nanobodies (Nbs) have arisen as an alternative to conventional antibodies (Abs) and show great potential when used as tools in different biotechnology fields such as diagnostics and therapy. Different approaches have been described for the production of Nbs and these methods face new challenges focused on improving yield, affinity, and reducing production costs. This review summarizes these challenges, and also the latest advances in the detection of different kinds of molecules, such as proteins and small molecules, and describes their potential use for noninvasive in vivo imaging and for in vitro assays. Read More

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http://dx.doi.org/10.1007/s00216-019-01633-4DOI Listing
February 2019
1 Read

Enantioseparation of chiral β-blockers using polynorepinephrine-coated nanoparticles and chiral capillary electrophoresis.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, Guangdong, China.

A method of combining magnetic solid-phase separation (MSPE) and chiral capillary electrophoresis (CE) is developed for enantioseparation of trace amounts of β-blockers. Polynorepinephrine-functionalized magnetic nanoparticles (polyNE-MNPs) are synthesized and applied to simultaneously extract three β-blockers (carteolol, metoprolol, and betaxolol). The prepared polyNE-MNPs are spherical with a diameter of 198 ± 17 nm and the thickness of the polyNE coating is about 14 nm. Read More

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http://dx.doi.org/10.1007/s00216-019-01641-4DOI Listing
February 2019

Programmable RNA-based systems for sensing and diagnostic applications.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, 00133, Rome, Italy.

The emerging field of RNA nanotechnology harnesses the versatility of RNA molecules to generate nature-inspired systems with programmable structure and functionality. Such methodology has therefore gained appeal in the fields of biosensing and diagnostics, where specific molecular recognition and advanced input/output processing are demanded. The use of RNA modules and components allows for achieving diversity in structure and function, for processing information with molecular precision, and for programming dynamic operations on the grounds of predictable non-covalent interactions. Read More

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http://dx.doi.org/10.1007/s00216-019-01622-7DOI Listing
February 2019

Solid-phase extraction of estrogens and herbicides from environmental waters for bioassay analysis-effects of sample volume on recoveries.

Anal Bioanal Chem 2019 Feb 8. Epub 2019 Feb 8.

Swiss Centre for Applied Ecotoxicology, Eawag-EPFL, Überlandstrasse 133, 8600, Dübendorf, Switzerland.

Ecotoxicological screening of surface waters can involve multiple analyses using multiple bioassay and chemical analytical methods that require enriched samples to reach low concentrations. Such broad screening of the same sample necessitates sufficient sample volume-typically several liters-to produce a sufficient amount of enriched sample. Often, this is achieved by performing parallel solid-phase extractions (SPE) where extracts are combined into a pool-this is a laborious process. Read More

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http://dx.doi.org/10.1007/s00216-019-01628-1DOI Listing
February 2019

An integrated proteomic and glycoproteomic study for differences on glycosylation occupancy in rheumatoid arthritis.

Anal Bioanal Chem 2019 Feb 7. Epub 2019 Feb 7.

Center for Diagnostics & Therapeutics and Department of Chemistry, Georgia State University, 50 Decatur St SE, Atlanta, GA, 30303, USA.

Rheumatoid arthritis (RA) is an autoimmune disease in which certain immune cells are dysfunctional and attack their own healthy tissues. There has been great difficulty in finding an accurate and efficient method for the diagnosis of early-stage RA. The present shortage of diagnostic methods leads to the rough treatments of the patients in the late stages, such as joint removing. Read More

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http://dx.doi.org/10.1007/s00216-018-1543-3DOI Listing
February 2019
2 Reads

Comparison of operator- and computer-controlled scanning electron microscopy of particles from different atmospheric aerosol types.

Anal Bioanal Chem 2019 Feb 6. Epub 2019 Feb 6.

Institute for Applied Geosciences, Technical University Darmstadt, Schnittspahnstraße 9, 64287, Darmstadt, Germany.

Individual aerosol particles from an urban background site in Mainz (Germany), a traffic hotspot site in Essen (Germany), the free troposphere in the Swiss Alps (high altitude research station Jungfraujoch), a rural background/marine site on Cyprus (Cyprus Atmospheric Observatory) and a rural background site in the forested area of Odenwald (Germany) were characterised with two different scanning electron microscopy techniques, operator controlled (opSEM) and computer controlled (ccSEM). For all samples, about 500 particles were investigated by opSEM, and between 1103 and 6940 particles by ccSEM. Large systematic differences (in some cases a factor up to ~ 20) in the abundance of the various particle groups are observed in the results of the two techniques. Read More

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http://dx.doi.org/10.1007/s00216-019-01614-7DOI Listing
February 2019
1 Read

Quantitative and rapid detection of amantadine and chloramphenicol based on various quantum dots with the same excitations.

Anal Bioanal Chem 2019 Feb 5. Epub 2019 Feb 5.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.

Herein, we developed a sensitive and quantitative flow assay for simultaneous detection of amantadine (AMD) and chloramphenicol (CAP) in chicken samples based on different CdSe/ZnS quantum dots (QDs). In contrast to other reports, the QDs could be excited by the same excitations that lowered the requirements for the matching instruments. Under the optimal conditions, the strategy permitted sensitive detection of AMD and CAP in a linear range of 0. Read More

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http://dx.doi.org/10.1007/s00216-019-01643-2DOI Listing
February 2019

Label-free detection of exosomes using a surface plasmon resonance biosensor.

Anal Bioanal Chem 2019 Feb 5. Epub 2019 Feb 5.

Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.

The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label-free detection of clinically relevant exosomes using a surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell-derived exosomes particularly from complex biological samples (e. Read More

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http://dx.doi.org/10.1007/s00216-019-01608-5DOI Listing
February 2019
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A new boronic acid reagent for the simultaneous determination of C-, C-, and C-brassinosteroids in plant tissues by chemical labeling-assisted liquid chromatography-mass spectrometry.

Anal Bioanal Chem 2019 Feb 4. Epub 2019 Feb 4.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, Hubei, China.

Brassinosteroids (BRs) are endogenous plant growth-promoting hormones affecting growth and development during the entire life cycle of plants. Naturally occurring BRs can be classified into C-, C-, or C-BRs based on the nature of the alkyl groups occupying the C-24 position in the side chain of the 5a-cholestane carbon skeleton. However, while C-BRs exhibit similar bioactivities to C- and C-BRs, the biosynthetic pathways of C-BRs in plants have not yet been clearly characterized. Read More

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http://dx.doi.org/10.1007/s00216-019-01612-9DOI Listing
February 2019
3.436 Impact Factor

Fast liquid chromatography-tandem mass spectrometry methodology for the analysis of alkylphenols and their ethoxylates in wastewater samples from the tank truck cleaning industry.

Anal Bioanal Chem 2019 Feb 4. Epub 2019 Feb 4.

Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, O&N II Herestraat 49, 3000, Leuven, Belgium.

A fast methodology to quantify 4-tert-octylphenol (4-t-OP) and 4-nonylphenol (4-NP) and their mono- and di-ethoxylates was developed, validated, and applied to real wastewater samples. Dispersive liquid-liquid microextraction was employed as a sample preparation step, leading to a pre-concentration factor of roughly 30. Analysis was carried out by liquid chromatography-tandem mass spectrometry with electrospray ionisation in multiple reaction monitoring mode. Read More

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http://dx.doi.org/10.1007/s00216-019-01623-6DOI Listing
February 2019
3.436 Impact Factor

Carbon isotope compositions of whole wine, wine solid residue, and wine ethanol, determined by EA/IRMS and GC/C/IRMS, can record the vine water status-a comparative reappraisal.

Anal Bioanal Chem 2019 Feb 4. Epub 2019 Feb 4.

Institute of Plant Production Sciences Agroscope, Viticulture Research Center, 1009, Pully, Switzerland.

Recently, we reported that the carbon isotope composition of the solid residues obtained by freeze-drying white and red wines (δC) could be used for tracing the water status of the vines whose grapes were used to produce them. Here, we compare different methods using δC values of other wine components, particularly those of whole wine (δC) obtained by elemental analysis and isotope ratio mass spectrometry (EA/IRMS) and of wine ethanol (δC) obtained by gas chromatography/combustion/IRMS (GC/C/IRMS), for their suitability to assess the vine water status. The studied wines were obtained from field-grown cultivars (Vitis vinifera L. Read More

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http://dx.doi.org/10.1007/s00216-019-01625-4DOI Listing
February 2019

Rapid determination of isocitrate dehydrogenase mutation status of human gliomas by extraction nanoelectrospray using a miniature mass spectrometer.

Anal Bioanal Chem 2019 Feb 2. Epub 2019 Feb 2.

Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.

Isocitrate dehydrogenase (IDH) I and II mutations in gliomas cause an abnormal accumulation of 2-hydroxyglutarate (2-HG) in these tumor cells. These mutations have potential prognostic value in that knowledge of the mutation status can lead to improved surgical resection. Information on mutation status obtained by immunohistochemistry or genomic analysis is not available during surgery. Read More

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http://link.springer.com/10.1007/s00216-019-01632-5
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http://dx.doi.org/10.1007/s00216-019-01632-5DOI Listing
February 2019
2 Reads

Toward an efficient workflow for the analysis of the human milk peptidome.

Anal Bioanal Chem 2019 Feb 2. Epub 2019 Feb 2.

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, The Netherlands.

There is a growing interest for investigating endogenous peptides from human biofluids which may provide yet unknown functional benefits or provide an early indication of disease states as potential biomarkers. A major technical bottleneck in the investigation of endogenous peptides from body fluids, e.g. Read More

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http://dx.doi.org/10.1007/s00216-018-01566-4DOI Listing
February 2019

Development and application of water-compatible molecularly imprinted polymers for the selective extraction of carbamazepine from environmental waters.

Anal Bioanal Chem 2019 Feb 2. Epub 2019 Feb 2.

Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), CNRS, ESPCI Paris, PSL Research University, 10 rue Vauquelin, 75231, Paris Cedex 05, France.

A molecularly imprinted polymer (MIP) was designed in order to allow the selective solid-phase extraction of carbamazepine (CBZ), an anticonvulsant and mood-stabilizing drug, at ultra-trace level from aqueous environmental samples. A structural analog of CBZ was selected as a dummy template and different synthesis conditions were screened. The selectivity of the resulting imprinted polymers was evaluated by studying the retention of CBZ in a solvent similar to the one used for the synthesis. Read More

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http://dx.doi.org/10.1007/s00216-019-01586-8DOI Listing
February 2019

Advanced methods for microRNA biosensing: a problem-solving perspective.

Anal Bioanal Chem 2019 Feb 2. Epub 2019 Feb 2.

Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, 95125, Catania, Italy.

MicroRNAs (miRNAs) present several features that make them more difficult to analyze than DNA and RNA. For this reason, efforts have been made in recent years to develop innovative platforms for the efficient detection of microRNAs. The aim of this review is to provide an overview of the sensing strategies able to deal with drawbacks and pitfalls related to microRNA detection. Read More

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http://dx.doi.org/10.1007/s00216-019-01621-8DOI Listing
February 2019

A novel coating method for CE capillary using carboxymethyl-β-cyclodextrin-modified magnetic microparticles as stationary for electrochromatography enantioseparation.

Anal Bioanal Chem 2019 Feb 1;411(6):1193-1202. Epub 2019 Feb 1.

Department of Analytical Chemistry, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing, 210009, Jiangsu, China.

Magnetic microparticles (MMPs) have been extensively studied and aroused considerable interest in separation science owing to their superior characteristics. In this paper, a novel coated capillary with carboxymethyl-β-cyclodextrin-functionalized magnetic microparticles (CD-MMPs) as stationary phase was constructed and then applied to establish an open-tubular capillary electrochromatography enantioseparation system. The preparation of the CD-MMP-coated open-tubular column was very convenient because the coating of the magnetic microparticles onto the capillary column could be easily manipulated by an external magnetic field. Read More

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http://dx.doi.org/10.1007/s00216-018-1545-1DOI Listing
February 2019

Structural signatures of the class III lasso peptide BI-32169 and the branched-cyclic topoisomers using trapped ion mobility spectrometry-mass spectrometry and tandem mass spectrometry.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St., AHC4-233, Miami, FL, 33199, USA.

Lasso peptides are a class of bioactive ribosomally synthesized and post-translationally modified peptides (RiPPs) characterized by a mechanically interlocked topology, where the C-terminal tail of the peptide is threaded and trapped within an N-terminal macrolactam ring. BI-32169 is a class III lasso peptide containing one disulfide bond that further stabilizes the lasso structure. In contrast to its branched-cyclic analog, BI-32169 has higher stability and is known to exert a potent inhibitory activity against the human glucagon receptor. Read More

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http://dx.doi.org/10.1007/s00216-019-01613-8DOI Listing
February 2019

Multifunctional N,S co-doped carbon dots for sensitive probing of temperature, ferric ion, and methotrexate.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Linyi Center for Disease Prevention and Control, Linyi, 276000, Shandong, China.

In this paper, we have presented a facile method to fabricate nitrogen and sulfur co-doped carbon dots (N,S-CDs) for blood methotrexate (MTX) sensing applications. The N,S-CDs with quantum yield up to 75% were obtained by one-step hydrothermal carbonization, using reduced glutathione and citric acid as the precursors. With this approach, the formation and the surface passivation of N,S-CDs were carried out simultaneously, resulting in intrinsic fluorescence emission. Read More

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http://dx.doi.org/10.1007/s00216-019-01617-4DOI Listing
February 2019

Shaping up field-deployable nucleic acid testing using microfluidic paper-based analytical devices.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Department of Chemistry, Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada.

Rapid, low-cost, and sensitive nucleic acid detection and quantification assays enabled by microfluidic paper-based analytical devices (μPADs) hold great promise for point-of-care disease diagnostics and field-based molecular tests. Through the capillary action in μPAD, flexible manipulation of nucleic acid samples can be achieved without the need for external pumps or power supplies, making it possible to fabricate highly integrated sample-to-answer devices that streamline the nucleic acid extraction, separation, concentration, amplification, and detection. To detect minute amounts of genetic materials from clinical and biological samples, it is also critical to develop sensitive signal readouts that generate physically detectable signals for in-device nucleic acid detection and/or quantification. Read More

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http://dx.doi.org/10.1007/s00216-019-01595-7DOI Listing
February 2019
3.436 Impact Factor

Fabrication of micro-patterned substrates for plasmonic sensing by piezo-dispensing of colloidal nanoparticles.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Leibniz Institute of Photonic Technology (IPHT) Jena, Member of the Leibniz Research Alliance - Leibniz Health Technologies, Albert-Einstein-Straße 9, 07745, Jena, Germany.

In this work we describe a very fast and flexible method for fabrication of plasmon-supporting substrates with micro-patterning capability, which is optimized for plasmonic sensing. We combined a wet chemistry approach to synthesize metallic nanoparticles with a piezo-dispensing system enabling deposition of nanoparticles on the substrates with micrometer precision. In this way, an arbitrary pattern consisting of 200 μm small spots containing plasmonic nanostructures can be produced. Read More

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http://dx.doi.org/10.1007/s00216-019-01587-7DOI Listing
February 2019

Development and strategy of reference materials for the DNA-based detection of genetically modified organisms.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, Hubei, China.

The enforcement of GMO labeling regulations requires validated analytical methods and certified reference materials (CRMs). The early labeling regulations stipulated that the GMO content should be expressed as percentage, but did not specify what unit this percentage referred to. Two reference systems, using mass fraction and copy number ratio as measurement units, individually, are established for GMO analysis using different metrological traceability chains. Read More

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http://dx.doi.org/10.1007/s00216-019-01576-wDOI Listing
February 2019
3.436 Impact Factor

Detection of multiclass explosives and related compounds in soil and water by liquid chromatography-dielectric barrier discharge ionization-mass spectrometry.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V., Bunsen-Kirchhoff-Straβe 11, 44139, Dortmund, Germany.

In this work, the multiclass detection of explosives and related compounds has been studied by liquid chromatography-high-resolution mass spectrometry by means of a time-of-flight mass spectrometer (TOFMS) operated in the negative ion mode, using dielectric barrier discharge ionization (DBDI). Reversed-phase high-performance liquid chromatography (HPLC) separation was performed using water-methanol mobile phase without any modifier, although the effect of ammonium acetate was studied. DBD plasma was generated by applying a square wave voltage of 2. Read More

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http://dx.doi.org/10.1007/s00216-019-01627-2DOI Listing
February 2019

Critical evaluation of the use of total reflection X-ray fluorescence spectrometry for the analysis of whole blood samples: application to patients with thyroid gland diseases.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia.

Multielemental analysis of whole blood can provide significant information for the evaluation of nutritional status and diagnosis of certain diseases as well as for the assessment of exposure to potentially toxic metals. However, the quantification of multiple elements in whole blood is not easy partly because of the wide variation in element concentrations (from ng L to g L) and the complex matrix. The aim of this work was to develop a fast, sustainable, and reliable analytical method, in combination with low-power TXRF, for multielemental analysis of blood samples. Read More

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http://dx.doi.org/10.1007/s00216-019-01618-3DOI Listing
February 2019
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A multichannel microchip containing 16 chambers packed with antibody-functionalized beads for immunofluorescence assay.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Xiangya School of Pharmaceutical Sciences, Central South University, 172 Tongzipo Road, Changsha, 410013, Hunan, China.

A multichannel chip containing 16 microchambers was developed for fast and sensitive immunoassays. In each chamber, antibody-functionalized nonmagnetic beads were applied as the solid phase to capture target antigens. Four types of IgGs (human, rabbit, chicken, and mouse) could be detected simultaneously by our combining this microchip with a sandwich immunoassay technique. Read More

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http://dx.doi.org/10.1007/s00216-019-01601-yDOI Listing
February 2019

Development of a one-step immunoassay for triazophos using camel single-domain antibody-alkaline phosphatase fusion protein.

Anal Bioanal Chem 2019 Feb 1;411(6):1287-1295. Epub 2019 Feb 1.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Resources and Environmental Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.

Triazophos is mainly used in Asian and African countries for the control of insects in agricultural production. Camelid variable domains of heavy-chain antibodies (VHHs) show great promise in monitoring environmental chemicals such as pesticides. To improve the rate of success in the generation of VHHs against triazophos, genes specifically encoding VHH fragments from the unique allotype IgG3a of an immunized Camelus bactrianus were amplified by using a pair of novel primers and introduced to construct a diverse VHH library. Read More

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http://dx.doi.org/10.1007/s00216-018-01563-7DOI Listing
February 2019

Nanofiber-integrated miniaturized systems: an intelligent platform for cancer diagnosis.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstrasse 31, 93053, Regensburg, Germany.

Cancer diagnostic tools enabling screening, diagnosis, and effective disease management are essential elements to increase the survival rate of diagnosed patients. Low abundance of cancer markers present in large amounts of interferences remains the major issue. Moreover, current diagnostic technologies are restricted to high-resourced settings only. Read More

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http://dx.doi.org/10.1007/s00216-019-01589-5DOI Listing
February 2019
2 Reads

Development of a UHPLC-MS method for inhibitor screening against α-L-1,3-fucosidase.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Key Laboratory of Marine Drugs, Chinese Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, Shandong, China.

α-L-Fucosidase (AFU) is a promising therapeutic target for the treatment of inflammation, cancer, cystic fibrosis, and fucosidosis. Some of the existing analytical methods for the assessment of AFU activity are lacking in sensitivity and selectivity, since most of them are based on spectrofluorimetric methods. More recently, mass spectrometry (MS) has evolved as a key technology for enzyme assays and inhibitor screening as it enables accurate monitoring of the conversion of substrate to product in enzymatic reactions. Read More

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http://dx.doi.org/10.1007/s00216-019-01575-xDOI Listing
February 2019
2 Reads

Clinical determination of folates: recent analytical strategies and challenges.

Anal Bioanal Chem 2019 Feb 1. Epub 2019 Feb 1.

Laboratory of Toxicology, Department of Bio-Analysis, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.

Since the introduction of liquid chromatography tandem mass spectrometry in clinical laboratories, folate analysis has shifted from microbiological or protein-binding assays to chromatographic methods. Now, it is possible to sensitively and selectively determine several folate species in clinical samples where only a total folate content could be quantified using a microbiological or a binding assay. Although several chromatographic methods have been developed, validated, and published, interlaboratory variability limits the comparability of the results. Read More

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http://dx.doi.org/10.1007/s00216-019-01574-yDOI Listing
February 2019
1 Read