13,847 results match your criteria Analytical and bioanalytical chemistry[Journal]


Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides.

Anal Bioanal Chem 2019 Apr 16. Epub 2019 Apr 16.

CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, Liaoning, China.

Because of structural flexibility and acid lability, the identification of phosphorylated lysine (pLys) peptides is a great challenge. We report here a cleavable hydrophobic derivatization (CHD) strategy for the enrichment and identification of pLys peptides. First, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate was synthesized to react with dephosphorylated lysine peptides, and then the derived peptides were captured by a C column, followed by cleavage of the hydrophobic chain, with the specific label left on the target peptides for further identification. Read More

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http://dx.doi.org/10.1007/s00216-019-01770-wDOI Listing
April 2019
3.436 Impact Factor

Rapid and noninvasive diagnostics of Huanglongbing and nutrient deficits on citrus trees with a handheld Raman spectrometer.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA.

Huanglongbing (HLB) or citrus greening is a devastating disease of citrus trees that is caused by the gram-negative Candidatus Liberibacter spp. bacteria. The bacteria are phloem limited and transmitted by the Asian citrus psyllid, Diaphorina citri, and the African citrus psyllid, Trioza erytreae, which allows for a wider dissemination of HLB. Read More

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http://link.springer.com/10.1007/s00216-019-01776-4
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http://dx.doi.org/10.1007/s00216-019-01776-4DOI Listing
April 2019
2 Reads

Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemistry and Polymer Science, University of Stellenbosch, Private Bag X1, Stellenbosch, 7602, South Africa.

Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties. However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS). The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0. Read More

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http://dx.doi.org/10.1007/s00216-019-01799-xDOI Listing

Coupling of micro-solid-phase extraction and internal extractive electrospray ionization mass spectrometry for ultra-sensitive detection of 1-hydroxypyrene and papaverine in human urine samples.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang, 330013, China.

Quantification of ultra-trace analytes in complex biological samples using micro-solid-phase extraction followed by direct detection with internal extractive electrospray ionization mass spectrometry (μSPE-iEESI-MS) was demonstrated. 1-Hydroxypyrene (1-OHP) and papaverine at attomole levels in human raw urine samples were analyzed under negative and positive ion detection mode, respectively. The μSPE was simply prepared by packing a disposable syringe filter with octadecyl carbon chain (C18)-bonded micro silica particles, which were then treated as the "bulk sample" after the analytes were efficiently enriched by the C18 particles. Read More

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http://dx.doi.org/10.1007/s00216-019-01794-2DOI Listing

Determination of sulfonamides in meat with dummy-template molecularly imprinted polymer-based chemiluminescence sensor.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 071000, Hebei, China.

In this study, a molecularly imprinted polymer capable of recognizing 15 sulfonamides was first synthesized with sulfabenz as the dummy template. The calculation results from computation simulation showed that the specific 3D conformation of the template had an important influence on the polymer's recognition ability. Then, the polymer was used as recognition reagent to prepare a chemiluminescence sensor on a conventional 96-well microplate for the determination of the residues of 15 sulfonamides in meat (chicken and pork). Read More

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http://dx.doi.org/10.1007/s00216-019-01792-4DOI Listing
April 2019
2 Reads

A micro-Raman and chemometric study of urinary tract infection-causing bacterial pathogens in mixed cultures.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Centre for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576104, India.

Detection of urinary tract infection (UTI)-causing bacteria uses conventional time-consuming microbiological techniques. The current need is to use a fast and reliable method of bacterial identification. In order to unambiguously distinguish the UTI-causing five bacterial species used in the current study, micro-Raman spectra were obtained from a home-assembled micro-Raman system and analyzed by multivariate statistical techniques such as principal component analysis (PCA), partial least square-discriminate analysis (PLS-DA), and support vector machine (SVM). Read More

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http://link.springer.com/10.1007/s00216-019-01784-4
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http://dx.doi.org/10.1007/s00216-019-01784-4DOI Listing
April 2019
1 Read

Characterization of the chemical composition of Drimia numidica plant parts using high-resolution mass spectrometry: study of their total phenolic content and antioxidant activity.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food Biotechnology and Development, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece.

Drimia species have been used since ancient times for their medicinal properties. Their bulbs are considered as the main source of secondary metabolites with biological activity but the chemical composition of the other plant parts has not yet been considered. The aim of this study is to contribute to the existing knowledge with new data on the total phenolic content, the antioxidant activity and the chemical profile of different parts of Drimia numidica. Read More

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http://dx.doi.org/10.1007/s00216-019-01781-7DOI Listing

Simple and green synthesis of carbon dots (CDs) from valerian root and application of modified mesoporous boehmite (AlOOH) with CDs as a fluorescence probe for determination of imipramine.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran.

A novel, sensitive, rapid, and simple fluorescent probe has been developed based on green-synthesized carbon dots (CDs). In this work, CDs have been synthesized from valerian root by hydrothermal method. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) results confirm the formation of CDs with sizes of less than 10 nm. Read More

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http://dx.doi.org/10.1007/s00216-019-01779-1DOI Listing

Standardization procedures for real-time breath analysis by secondary electrospray ionization high-resolution mass spectrometry.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

University Children's Hospital Basel, University of Basel, Spitalstr. 33, 4056, Basel, Switzerland.

Despite the attractiveness of breath analysis as a non-invasive means to retrieve relevant metabolic information, its introduction into routine clinical practice remains a challenge. Among all the different analytical techniques available to interrogate exhaled breath, secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) offers a number of advantages (e.g. Read More

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http://dx.doi.org/10.1007/s00216-019-01764-8DOI Listing

Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemistry, Zhejiang University, Hangzhou, 310027, Zhejiang, China.

Oligonucleotides are usually analyzed by ion-pair reversed-phase liquid chromatography (IP-RPLC) coupled with negative mode electrospray ionization mass spectrometry (ESI-MS) due to their highly negative charged phosphodiester backbones. Herein, the signal suppression effect of triethylamine (TEA) adducts caused the ion-pair reagent TEA/hexafluoroisopropanol (HFIP) is greatly alleviated after improving the in-source energy in positive mode ESI-MS. This strategy is applied for different RNA sequencing through analyzing their formic acid hydrolysates via IP-RPLC MS. Read More

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http://dx.doi.org/10.1007/s00216-019-01819-wDOI Listing

Magnetic nanocomposites: versatile tool for the combination of immunomagnetic separation with flow-based chemiluminescence immunochip for rapid biosensing of Staphylococcal enterotoxin B in milk.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Marchioninistraße 17, 81377, Munich, Germany.

Immunomagnetic separation (IMS) was combined with flow-based chemiluminescence sandwich immunoassays (CL-SIA) for the quantification of Staphylococcal enterotoxin B in milk. Therefore, iron oxide-shell silica-core magnetic nanocomposites were conjugated to biotinylated anti-SEB antibodies (MNC-IgGs). MNC-IgGs were applied successfully for (i) capturing SEB in milk samples by an affinity reaction, (ii) magnetophoretic collection on antibody spots in a channel of a flow-based immunochip, and (iii) sensitive enzymatic chemiluminescence detection of biotin labels by poly(horseradish peroxidase)-streptavidin. Read More

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http://dx.doi.org/10.1007/s00216-019-01808-zDOI Listing

Discrimination of the species and authenticity of Rhizoma Coptidis based on stable isotope and multielement fingerprinting and multivariate statistical analysis.

Anal Bioanal Chem 2019 Apr 15. Epub 2019 Apr 15.

College of Pharmacy, Chongqing Medical University, Chongqing, 400016, China.

It is essential to be able to identify the source species and to determine the authenticity of traditional Chinese medicines (TCM) in order to prevent the use of false or inferior medicines. In this work, a stable and reliable method of discriminating among the three source species of Rhizoma Coptidis and checking the authenticity of Rhizoma Coptidis samples was established. The technique involved evaluating stable isotope ratios and the contents of multiple elements in samples along with the use of multivariate statistical techniques. Read More

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http://dx.doi.org/10.1007/s00216-019-01723-3DOI Listing
April 2019
3.436 Impact Factor

Trends in mass spectrometry imaging for cardiovascular diseases.

Anal Bioanal Chem 2019 Apr 12. Epub 2019 Apr 12.

Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.

Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, MALDI-based approaches have been applied to obtain in-depth knowledge of cardiac (dys)function. Read More

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http://dx.doi.org/10.1007/s00216-019-01780-8DOI Listing

Trace analysis of UV filters and musks in living fish by in vivo SPME-GC-MS.

Anal Bioanal Chem 2019 Apr 11. Epub 2019 Apr 11.

Facultad de Medicina Veterinaria y Zootecnia, Departamento de Abejas, Conejos y Organismos Acuáticos, Universidad Nacional Autónoma de México, 04510, Ciudad de México, Mexico.

A method was developed for the simultaneous determination of two groups of personal care products, namely UV filters (oxybenzone, 3-(4-methylbenzylidene)camphor, padimate-O, 2-ethylhexyl-4-methoxycinnamate, and octocrylene) and polycyclic aromatic musks (galaxolide and tonalide), in fish by in vivo solid-phase microextraction followed by gas chromatography-mass spectrometry. The in vivo method was validated by carrying out in vitro experiments; the method validation parameters were linearity (r > 0.98), interday precision (relative standard deviations < 35. Read More

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http://link.springer.com/10.1007/s00216-019-01791-5
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http://dx.doi.org/10.1007/s00216-019-01791-5DOI Listing
April 2019
1 Read

Functional MoS nanosheets for precursor and mature microRNA detection in living cells.

Anal Bioanal Chem 2019 Apr 11. Epub 2019 Apr 11.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing, 100048, China.

Mature microRNAs (miRNAs) are small-sized RNAs cleaved from precursor microRNAs (pre-miRNAs) by the RNase Dicer. Various miRNAs play key regulatory roles in tumorigenesis and metastasis, and are therefore potential diagnostic and prognostic cancer biomarkers. However, detecting miRNAs and pre-miRNAs accurately and selectively in living cells remains a major challenge, as the mature miRNA sequence is also present in its pre-miRNA and current sequence probes exhibit poor gene delivery efficiency. Read More

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http://link.springer.com/10.1007/s00216-019-01753-x
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http://dx.doi.org/10.1007/s00216-019-01753-xDOI Listing
April 2019
3 Reads

A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood.

Anal Bioanal Chem 2019 Apr 11. Epub 2019 Apr 11.

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan, 430079, Hubei, China.

Blood counting is one of the most commonly ordered clinical assays, and is often part of the basis for initial diagnosis and screening for disease. While substantial prior research has shown the ability of portable instruments to accurately produce blood counts through image- or flow-based cytometry, these methods require complex sample preparation using either costly commercial imaging chambers or complicated reagents. To address these issues, in this paper we developed a method to prepare trace volumes of whole blood aimed at portable blood counting. Read More

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http://dx.doi.org/10.1007/s00216-019-01738-wDOI Listing
April 2019
3.436 Impact Factor

Time-integrated thermal desorption for quantitative SIFT-MS analyses of atmospheric monoterpenes.

Anal Bioanal Chem 2019 Apr 11. Epub 2019 Apr 11.

The Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry, Dolejškova 3, 18223, Prague 8, Czech Republic.

A new time-integrated thermal desorption technique has been developed that can be used with selected ion flow tube mass spectrometry, TI-TD/SIFT-MS, for off-line quantitative analyses of VOCs accumulated onto sorbents. Using a slow desorption temperature ramp, the absolute amounts of desorbed compounds can be quantified in real time by SIFT-MS and constitutional isomers can be separated. To facilitate application of this technique to environmental atmospheric monitoring, method parameters were optimised for quantification of the three common atmospheric monoterpenes: β-pinene, R-limonene and 3-carene. Read More

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http://link.springer.com/10.1007/s00216-019-01782-6
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http://dx.doi.org/10.1007/s00216-019-01782-6DOI Listing
April 2019
2 Reads

High-performance bioanalysis based on ion concentration polarization of micro-/nanofluidic devices.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, Jiangsu, China.

Micro-/nanofluidics has received considerable attention over the past two decades, which allows efficient biomolecule trapping and preconcentration due to ion concentration polarization (ICP) within nanostructures. The rich scientific content related to ICP has been widely exploited in different applications including protein concentration, biomolecules sensing and detection, cell analysis, and water purification. Compared to pure microfluidic devices, micro-/nanofluidic devices show a highly efficient sample enrichment capacity and nonlinear electrokinetic flow feature. Read More

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http://link.springer.com/10.1007/s00216-019-01756-8
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http://dx.doi.org/10.1007/s00216-019-01756-8DOI Listing
April 2019
1 Read

Using differential ion mobility spectrometry to perform class-specific ion-molecule reactions of 4-quinolones with selected chemical reagents.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

Bioanalytical Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany.

Gas phase ion/molecule reactions are often used in analytical applications to support the analysis of isomers or to identify specific functional groups of organic molecules. Until now, deliberate chemical reactions have not been performed in differential ion mobility spectrometry (DMS) devices except for hydrogen exchange and cluster formation. The present work extends that of Colorado and Brodbelt (Anal Chem 66:2330-5, 1994) on ion/molecule reactions in an ion trap mass spectrometer. Read More

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http://dx.doi.org/10.1007/s00216-019-01789-zDOI Listing

Black oxidized 3,3',5,5'-tetramethylbenzidine nanowires (oxTMB NWs) for enhancing Pt nanoparticle-based strip immunosensing.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and College of Chemistry, Fuzhou University, No. 2, Xueyuan Road, Fuzhou, 350108, Fujian, China.

It is well known that 3,3',5,5'-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. Read More

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http://dx.doi.org/10.1007/s00216-019-01745-xDOI Listing

Proposing a validation scheme for C metabolite tracer studies in high-resolution mass spectrometry.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Str. 38, 1090, Vienna, Austria.

C metabolite tracer and metabolic flux analyses require upfront experimental planning and validation tools. Here, we present a validation scheme including a comparison of different LC methods that allow for customization of analytical strategies for tracer studies with regard to the targeted metabolites. As the measurement of significant changes in labeling patterns depends on the spectral accuracy, we investigate this aspect comprehensively for high-resolution orbitrap mass spectrometry combined with reversed-phase chromatography, hydrophilic interaction liquid chromatography, or anion-exchange chromatography. Read More

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http://dx.doi.org/10.1007/s00216-019-01773-7DOI Listing

Development of a stable chemically cross-linked erythropoietin dimer for use in the quality control of erythropoietin therapeutic products.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

National Institute for Biological Standards & Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK.

Erythropoietin (EPO) is a glycoprotein hormone which promotes red cell replenishment and is also a global biotherapeutic medicine widely used to treat anaemia resulting, for example, from chemotherapy. Requirements of the European Pharmacopoeia stipulate that the level of dimer must be quantified in clinical EPO products (with a limit of 2%). Quantification is hampered by the lack of reference preparations containing stable measurable levels of EPO dimer, but the reproducible generation of a stable dimerised EPO preparation is challenging. Read More

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http://dx.doi.org/10.1007/s00216-019-01768-4DOI Listing
April 2019
1 Read

Determination of berberine-upregulated endogenous short-chain fatty acids through derivatization by 2-bromoacetophenone.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, 100050, China.

Short-chain fatty acids (SCFAs) are a major group of endogenous metabolites generated by the gut microbiota and have been reported to play an important role in physical health, such as improving energy metabolism. Here, using 2-bromoacetophenone as the derivatization reagent (BP, 10 mg/mL, 40 °C for 20 min), a sensitive liquid chromatography-tandem mass spectrometric method was established for the quantitative determination of seven short-chain fatty acids in plasma and feces. The analyses were performed on a C column in positive multiple reaction monitoring mode. Read More

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http://dx.doi.org/10.1007/s00216-019-01793-3DOI Listing
April 2019
2 Reads

Prêt-à-porter nanoYESα and nanoYESβ bioluminescent cell biosensors for ultrarapid and sensitive screening of endocrine-disrupting chemicals.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.

Cell-based assays utilizing reporter gene technology have been widely exploited for biosensing, as they provide useful information about the bioavailability and cell toxicity of target analytes. The long assay time due to gene transcription and translation is one of the main drawbacks of cell biosensors. We report the development of two yeast biosensors stably expressing human estrogen receptors α and β and employing NanoLuc as the reporter protein to upgrade the widely used yeast estrogen screening (YES) assays. Read More

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http://dx.doi.org/10.1007/s00216-019-01805-2DOI Listing

Solution to fading lemonade challenge.

Authors:
Hervé This

Anal Bioanal Chem 2019 Mar;411(9):1677-1678

INRA, UMR 1145 Ingénierie Procédés Aliments, Group of Molecular Gastronomy, 16 rue Claude Bernard, 75005, Paris, France.

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http://dx.doi.org/10.1007/s00216-019-01591-xDOI Listing

Lead quantitation challenge.

Authors:
Enea Pagliano

Anal Bioanal Chem 2019 Mar;411(9):1675-1676

National Research Council Canada, 1200 Montreal Road, K1A 0R6, Ottawa, ON, Canada.

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http://dx.doi.org/10.1007/s00216-019-01592-wDOI Listing

Microporous polymer based on the new compound "bi-(4-vinyl phenylquinoline) amide" for enrichment and quantitative determination of lamotrigine in rat and human serum.

Anal Bioanal Chem 2019 Apr 8. Epub 2019 Apr 8.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.

Lamotrigine is one of the most widely used antiepileptic drugs in the treatment of epilepsy. This kind of drug needs to be used in the long term and should be quantitatively detected in the blood of patients to avoid drug toxicity caused by individual differences and environmental and pathological changes in the process of taking. The detection of antiepileptic drugs in human blood is challenging because of their low contents and the interference of complex matrices. Read More

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http://dx.doi.org/10.1007/s00216-019-01812-3DOI Listing
April 2019
1 Read

Ultra-high-resolution ion mobility spectrometry-current instrumentation, limitations, and future developments.

Anal Bioanal Chem 2019 Apr 8. Epub 2019 Apr 8.

Institute of Electrical Engineering and Measurement Technology, Department of Sensors and Measurement Technology, Leibniz Universität Hannover, Appelstr. 9A, 30167, Hannover, Germany.

With recent advances in ionization sources and instrumentation, ion mobility spectrometers (IMS) have transformed from a detector for chemical warfare agents and explosives to a widely used tool in analytical and bioanalytical applications. This increasing measurement task complexity requires higher and higher analytical performance and especially ultra-high resolution. In this review, we will discuss the currently used ion mobility spectrometers able to reach such ultra-high resolution, defined here as a resolving power greater than 200. Read More

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http://dx.doi.org/10.1007/s00216-019-01807-0DOI Listing

Synthesis, characterization, and application of chemically interconnected carbon nanotube monolithic sorbents by photopolymerization in polypropylene caps.

Anal Bioanal Chem 2019 Apr 8. Epub 2019 Apr 8.

Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, 14071, Córdoba, Spain.

A facile and convenient approach for the preparation of interconnected multiwalled carbon nanotube (MWCNT) monolithic sorbents in recycled plastic caps has been developed. The method, which was based on the photopolymerization of the individual MWCNTs via the formation of a W/O medium internal phase emulsion (40/60 w/w%), provides control over the size of pores, rigidity, and the mechanical stability of the final solid. Pluronic L121 was used as a surfactant containing the water phase inside it and, consequently, the organic and non-polar phase, in which the MWCNTs and the cross-linker were trapped, remained on the outside of the droplets. Read More

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http://dx.doi.org/10.1007/s00216-019-01795-1DOI Listing

Detection of very long-chain hydrocarbons by laser mass spectrometry reveals novel species-, sex-, and age-dependent differences in the cuticular profiles of three Nasonia species.

Anal Bioanal Chem 2019 Apr 8. Epub 2019 Apr 8.

Institute for Hygiene, University of Münster, Robert-Koch-Str. 41, 48149, Münster, Germany.

Long-chain cuticular hydrocarbons (CHC) are key components of chemical communication in many insects. The parasitoid jewel wasps from the genus Nasonia use their CHC profile as sex pheromone and for species recognition. The standard analytical tool to analyze CHC is gas chromatography coupled with mass spectrometric detection (GC/MS). Read More

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http://dx.doi.org/10.1007/s00216-019-01736-yDOI Listing

Electrochemical DNA biosensors for label-free breast cancer gene marker detection.

Anal Bioanal Chem 2019 Apr 8. Epub 2019 Apr 8.

, Dabska 18H 52-571, Kraków, Poland.

We present an electrochemical DNA detection strategy based on self-assembled ferrocene-cored poly(amidoamine) dendrimers for the detection of a gene relevant to breast cancer. The chemisorption of three ferrocene-cored poly(amidoamine) generations and hybridization of single-stranded DNA on a Au electrode were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor demonstrated high sensitivity of 0. Read More

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http://dx.doi.org/10.1007/s00216-019-01739-9DOI Listing
April 2019
1 Read

A microanalytical capillary electrophoresis mass spectrometry assay for quantifying angiotensin peptides in the brain.

Anal Bioanal Chem 2019 Apr 5. Epub 2019 Apr 5.

Department of Chemistry & Biochemistry, University of Maryland, 8051 Regents Drive, College Park, MD, 20742, USA.

The renin-angiotensin system (RAS) of the brain produces a series of biologically active angiotensinogen-derived peptides involved in physiological homeostasis and pathophysiology of disease. Despite significant research efforts to date, a comprehensive understanding of brain RAS physiology is lacking. A significant challenge has been the limited set of bioanalytical assays capable of detecting angiotensin (Ang) peptides at physiologically low concentrations (2-15 fmol/g of wet tissue) and sufficient chemical specificity for unambiguous molecular identifications. Read More

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http://link.springer.com/10.1007/s00216-019-01771-9
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http://dx.doi.org/10.1007/s00216-019-01771-9DOI Listing
April 2019
4 Reads

Determination of fluoroquinolones in food samples by magnetic solid-phase extraction based on a magnetic molecular sieve nanocomposite prior to high-performance liquid chromatography and tandem mass spectrometry.

Anal Bioanal Chem 2019 Apr 2. Epub 2019 Apr 2.

Department of Chemistry, The Chinese University of Hong Kong, New Territories, HKSAR, China.

In this study, a magnetic molecular sieve material (FeO@MCM-48) was synthesized by a combination of solvothermal and self-assembly methods. The physicochemical properties of the magnetic molecular sieve material were characterized by scanning electron microscopy, energy-dispersive spectroscopy, magnetic hysteresis loop measurements, transmission electron microscopy, powder X-ray diffraction, N adsorption-desorption analysis, and Fourier transform infrared spectroscopy. The as-synthesized nanocomposite showed various advantages, including easy magnetic-assisted separation, high specific surface area, and a highly interwoven and branched mesoporous structure. Read More

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http://dx.doi.org/10.1007/s00216-019-01726-0DOI Listing
April 2019
1 Read

Origins of the method of standard additions and of the use of an internal standard in quantitative instrumental chemical analyses.

Anal Bioanal Chem 2019 Apr 2. Epub 2019 Apr 2.

Laboratory of the Government Chemist, Teddington, Middlesex, TW, 11 0LY, UK.

The origin of the method of standard additions (SAM) and of the use of internal standard (IS) in instrumental chemical analysis and their spread into other areas has been reviewed. Recorded applications of IS range from flame spectroscopy in 1877 through multiple techniques to current use in NMR and standard additions with isotopically labelled internal standards in hyphenated techniques. For SAM, applications stemmed from polarography in 1937 and spread to most instrumental methods. Read More

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http://dx.doi.org/10.1007/s00216-019-01754-wDOI Listing

Application of differential mobility-mass spectrometry for untargeted human plasma metabolomic analysis.

Anal Bioanal Chem 2019 Apr 2. Epub 2019 Apr 2.

Department of Internal Medicine, Division of Nephrology, University of Michigan, Ann Arbor, MI, 48105, USA.

Differential mobility spectrometry (DMS) has been gaining popularity in small molecule analysis over the last few years due to its selectivity towards a variety of isomeric compounds. While DMS has been utilized in targeted liquid chromatography-mass spectrometry (LC-MS), its use in untargeted discovery workflows has not been systematically explored. In this contribution, we propose a novel workflow for untargeted metabolomics based solely on DMS separation in a clinically relevant chronic kidney disease (CKD) patient population. Read More

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http://dx.doi.org/10.1007/s00216-019-01719-zDOI Listing
April 2019
1 Read

Salivary diagnostics on paper microfluidic devices and their use as wearable sensors for glucose monitoring.

Anal Bioanal Chem 2019 Apr 2. Epub 2019 Apr 2.

Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, Goiânia, GO, 74690-900, Brazil.

Microfluidic paper-based devices (μPADs) and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrument-free fast results. Diseases such as periodontitis and diabetes mellitus can potentially be detected through these devices by the detection of important biomarkers. This study describes the development of μPADs through craft cutter printing for glucose and nitrite salivary diagnostics. Read More

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http://dx.doi.org/10.1007/s00216-019-01788-0DOI Listing
April 2019
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Green synthesis of carbon dots using the flowers of Osmanthus fragrans (Thunb.) Lour. as precursors: application in Fe and ascorbic acid determination and cell imaging.

Anal Bioanal Chem 2019 May 2;411(12):2715-2727. Epub 2019 Apr 2.

School of Pharmacy, Southwest Medical University, Luzhou, 646000, Sichuan, China.

In this work, dried flowers of Osmanthus fragrans Lour. were applied as green precursors to synthesize carbon dots (CDs) by a green hydrothermal method for the first time. The CDs showed strong blue fluorescence at 410 nm under 340-nm excitation with a quantum yield of approximately 18. Read More

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http://dx.doi.org/10.1007/s00216-019-01712-6DOI Listing

Osteoporosis risk assessment using multilayered gold-nanoparticle thin film via SALDI-MS measurement.

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan.

A powerful technique to detect bone biomarkers has been developed for assessment of osteoporosis at the early stage. Two-dimensional multilayered gold-nanoparticle thin film (MTF-AuNPs) was demonstrated as a promising test platform for detection of bone biomarker, hydroxyproline (HYP), measured by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). With strong surface plasmon resonance and excellent homogeneity, facilely prepared, highly ordered, and large-scale MTF-AuNPs revealed high sensitivity of HYP in the SALDI-MS measurement without additional matrixes, such as α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB). Read More

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http://dx.doi.org/10.1007/s00216-019-01759-5DOI Listing
April 2019
2 Reads

Selective chiral recognition of alanine enantiomers by chiral calix[4]arene coated quartz crystal microbalance sensors.

Anal Bioanal Chem 2019 May 1;411(12):2675-2685. Epub 2019 Apr 1.

Department of Chemical Engineering, Konya Technical University, 42130, Konya, Turkey.

We describe the synthesis of new chiral calix[4]arene derivatives having (R)-1-phenylethylamine, (S)-1-phenylethylamine, (R)-2-phenylglycinol, and (S)-2-phenylglycinol moieties, and chiral recognition studies for enantiomers of some selected α-amino acid derivatives such as alanine, phenylalanine, serine, and tryptophan using a quartz crystal microbalance (QCM). Initial experiments indicated that the highest selective chiral recognition factor was 1.42 for alanine enantiomers. Read More

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http://dx.doi.org/10.1007/s00216-019-01705-5DOI Listing

Spectroscopic and electrophoresis study of substitution on the surface of gold nanoparticles by different mercaptoalkyl carboxylic acids and bioconjugation with bovine serum albumin.

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

University of Sao Paulo, Av. Prof. Lineu Prestes 748, São Paulo, SP, 05508-000, Brazil.

To develop bioconjugated materials, it is necessary to understand how the various elements present in a conjugate interact with one another. To gain insights into nanoparticle-capping agent-protein interactions, gold nanoparticles (AuNPs) measuring 30 nm in diameter were coated with different molecules bearing a thiol group: 3-mercaptopropionic acid, 6-mercaptohexanoic acid, and 11-mercaptoundecanoic acid. The covalent conjugation of AuNPs to the protein bovine serum albumin (BSA) via a cross-linker reaction with N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide was systematically investigated under different reaction conditions with variation of the concentrations of the mercaptoalkyl carboxylic acid (MA) and BSA. Read More

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http://dx.doi.org/10.1007/s00216-019-01758-6DOI Listing
April 2019
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Salvatore Fanali, Paul R. Haddad, Colin F. Poole, Marja-Liisa Riekkola (Eds.): Liquid chromatography: applications, 2nd ed.

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

Chemical Sciences Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899-8392, USA.

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http://dx.doi.org/10.1007/s00216-019-01729-xDOI Listing

Analysis and fate of 14 relevant wastewater-derived organic pollutants in long-term exposed soil.

Anal Bioanal Chem 2019 May 1;411(12):2687-2696. Epub 2019 Apr 1.

Department of Environmental Chemistry, Institute for Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18-26, 08014, Barcelona, Spain.

The growing use of reclaimed water in agriculture worldwide calls for developing high-sensitivity methods to quantify wastewater-derived organic contaminants in soils so that the potential risk of this irrigation practice can be properly assessed. This work describes an analytical method for the determination of trace levels of 14 drugs that are known to be poorly removed during conventional wastewater treatment in soil. The analytes selected for investigation included ten pharmaceuticals from different therapeutic classes (carbamazepine, diclofenac, cis-diltiazem, lamotrigine, methadone, midazolam, oxcarbazepine, sulfamethoxazole, trimethoprim, valsartan), one illicit drug (cocaine), and three transformation products/metabolites (acridone, 4'-hydroxydiclofenac, and valsartan acid), thereby covering a broad range of physical-chemical properties. Read More

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http://dx.doi.org/10.1007/s00216-019-01715-3DOI Listing

Extending the scope of dispersive liquid-liquid microextraction for trace analysis of 3-methyl-1,2,3-butanetricarboxylic acid in atmospheric aerosols leading to the discovery of iron(III) complexes.

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

Department of Chemistry, Center for Analysis and Synthesis, Lund University, P.O. Box 124, 221 00, Lund, Sweden.

3-Methyl-1,2,3-butanetricarboxylic acid (MBTCA) is a secondary organic aerosol and can be used as a unique emission marker of biogenic emissions of monoterpenes. Seasonal variations and differences in vegetation cover around the world may lead to low atmospheric MBTCA concentrations, in many cases too low to be measured. Hence, an important tool to quantify the contribution of terrestrial vegetation to the loading of secondary organic aerosol may be compromised. Read More

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http://link.springer.com/10.1007/s00216-019-01741-1
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http://dx.doi.org/10.1007/s00216-019-01741-1DOI Listing
April 2019
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Richard G. Brereton: Chemometrics: data driven extraction for science, 2nd ed.

Authors:
Thomas Bocklitz

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

Institute of Physical Chemistry and Abbe Center of Photonics (IPC), Friedrich-Schiller-University, Helmholtzweg 4, 07743, Jena, Germany.

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http://dx.doi.org/10.1007/s00216-019-01786-2DOI Listing
April 2019
5 Reads

Salvatore Fanali, Paul R. Haddad, Colin F. Poole, Marja-Liisa Riekkola (Eds.): Liquid chromatography: fundamentals and instrumentation, 2nd ed.

Anal Bioanal Chem 2019 Apr 1. Epub 2019 Apr 1.

Chemical Sciences Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899-8392, USA.

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http://dx.doi.org/10.1007/s00216-019-01728-yDOI Listing
April 2019
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Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling.

Anal Bioanal Chem 2019 Mar 30. Epub 2019 Mar 30.

Department of Chemistry and Biochemistry, The University of Toledo, 2801 W. Bancroft, Toledo, OH, 43606, USA.

G protein-coupled receptors (GPCRs), G proteins, and their signaling associates are major signal transducers that control the majority of cellular signaling and regulate key biological functions including immune, neurological, cardiovascular, and metabolic processes. These pathways are targeted by over one-third of drugs on the market; however, the current understanding of their function is limited and primarily derived from cell-destructive approaches providing an ensemble of static, multi-cell information about the status and composition of molecules. Spatiotemporal behavior of molecules involved is crucial to understanding in vivo cell behaviors both in health and disease, and the advent of genetically encoded fluorescence proteins and small fluorophore-based biosensors has facilitated the mapping of dynamic signaling in cells with subcellular acuity. Read More

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http://dx.doi.org/10.1007/s00216-019-01774-6DOI Listing
March 2019
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Simultaneous detection of nicotinamide adenine nucleotides and adenylate pool to quantify redox and energy states in mAb-producing CHO cells by capillary electrophoresis.

Anal Bioanal Chem 2019 Mar 28. Epub 2019 Mar 28.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Box 309, 130 Meilong Road, Shanghai, 200237, China.

Chinese hamster ovary (CHO) cells are predominant in the production of therapeutic proteins to treat various diseases. Characterization and investigation of CHO cell metabolism in a quick and simple way could boost process and cell line development. Therefore, a method to simultaneously detect seven redox- and energy-related metabolites in CHO cells by capillary electrophoresis has been developed. Read More

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http://dx.doi.org/10.1007/s00216-019-01747-9DOI Listing
March 2019
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A chiral unified chromatography-mass spectrometry method to analyze free amino acids.

Anal Bioanal Chem 2019 Mar 28. Epub 2019 Mar 28.

University of Orleans, ICOA, CNRS UMR 7311, rue de Chartres, BP 6759, 45067, Orléans, France.

In this project, we aimed at analyzing native (or free) amino acids with supercritical fluid chromatography coupled to mass spectrometric detection, with modern instruments and methods, and maintaining as simple a mobile phase as possible to ensure applicability of the method. The purpose was twofold: (i) a generic method allowing for satisfactory elution of a wide range of amino acids (acidic, basic, or neutral residue) and (ii) resolution of the enantiomeric pairs. The Chiralpak ZWIX (+) and (-) stationary phases were selected as they are well-known for the enantioresolution of amino acids in liquid chromatographic modes. Read More

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http://dx.doi.org/10.1007/s00216-019-01783-5DOI Listing
March 2019
2 Reads
3.436 Impact Factor

Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor.

Anal Bioanal Chem 2019 Mar 28. Epub 2019 Mar 28.

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", 70125, Bari, Italy.

In the last decade, saliva has been suggested as non-invasive diagnostic fluid, suitable for clinical use alternatively to blood serum and plasma. However, the clinical applicability of saliva has been hampered so far by the inadequate sensitivity of current methods to detect the lower salivary concentrations of many biomarkers monitored in blood products. Herein, a label-free biosensor based on electrolyte-gated organic thin-film transistor (EGOTFT) has been developed for the detection at the physical limit of C-reactive protein (CRP) in human saliva. Read More

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http://dx.doi.org/10.1007/s00216-019-01778-2DOI Listing

Correction to: Three-dimensional reconstruction of the distribution of elemental tags in single cells using laser ablation ICP-mass spectrometry via registration approaches.

Anal Bioanal Chem 2019 Mar 28. Epub 2019 Mar 28.

Atomic & Mass Spectrometry (A&MS) research unit, Department of Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, 9000, Ghent, Belgium.

The authors would like to call the reader's attention to the fact that unfortunately the originally provided affiliation for Dr. Tomoko Asaoka was not correct. Read More

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http://dx.doi.org/10.1007/s00216-019-01772-8DOI Listing
March 2019
1 Read