14,732 results match your criteria Analytical and bioanalytical chemistry[Journal]


Development of an LC-MS method for determination of nitrogen-containing heterocycles using mixed-mode liquid chromatography.

Anal Bioanal Chem 2020 May 26. Epub 2020 May 26.

Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstraße 5, 45141, Essen, Germany.

N-containing heterocycles (NCHs) are largely used as precursors for pharmaceuticals and can enter the environment. Some NCHs have been shown to be toxic, persistent, and very mobile in the environment. Thus, they have received increasing attention in the past years. Read More

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http://dx.doi.org/10.1007/s00216-020-02665-xDOI Listing

Estrogenic activity of food contact materials-evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates.

Anal Bioanal Chem 2020 May 26. Epub 2020 May 26.

Swiss Centre for Applied Ecotoxicology, Eawag, Überlandstrasse 133, 8600, Dübendorf, Switzerland.

Food contact materials (FCM) may contain complex mixtures of estrogenic chemicals. A yeast estrogen screen performed on high performance thin-layer chromatography plates (planar-YES, P-YES) is promising for analysis of such mixtures, as it could allow for better elucidation of effects compared with established methods in microtiter plates. However, the P-YES has not been directly compared with established methods. Read More

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http://dx.doi.org/10.1007/s00216-020-02701-wDOI Listing

Monitoring cell endocytosis of liposomes by real-time electrical impedance spectroscopy.

Anal Bioanal Chem 2020 May 25. Epub 2020 May 25.

Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.

Evaluation and understanding the effect of drug delivery in in vitro systems is fundamental in drug discovery. We present an assay based on real-time electrical impedance spectroscopy (EIS) measurements that can be used to follow the internalisation and cytotoxic effect of a matrix metalloproteinase (MMP)-sensitive liposome formulation loaded with oxaliplatin (OxPt) on colorectal cancer cells. The EIS response identified two different cellular processes: (i) a negative peak in the cell index (CI) within the first 5 h, due to onset of liposome endocytosis, followed by (ii) a subsequent CI increase, due to the reattachment of cells until the onset of cytotoxicity with a decrease in CI. Read More

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http://dx.doi.org/10.1007/s00216-020-02592-xDOI Listing

Salinomycin-loaded PLA nanoparticles: drug quantification by GPC and wave voltammetry and biological studies on osteosarcoma cancer stem cells.

Anal Bioanal Chem 2020 May 25. Epub 2020 May 25.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166, Messina, Italy.

A new straightforward gel permeation chromatography (GPC) method was developed to calculate the drug encapsulation efficiency and loading content of Poly(lactic acid) nanoparticles (PLA NPs) loaded with Salinomycin (Sal), exploiting the capability of this technique to separate a macromolecular/molecular mixture on the basis of the molecular weight of each component. The proposed GPC method allowed Sal detection until 1% of Sal content in PLA NPs, avoiding sample pre-treatments. The method was validated by wave voltammetry (SW) technique, using a slightly modified literature procedure, useful to detect Sal in the concentration range 0. Read More

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http://dx.doi.org/10.1007/s00216-020-02721-6DOI Listing

Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.

Anal Bioanal Chem 2020 May 25. Epub 2020 May 25.

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA.

High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world. On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements. These proportions vary depending on the type of potato and location where they were cultivated. Read More

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http://dx.doi.org/10.1007/s00216-020-02706-5DOI Listing

Improving the chromatographic selectivity of β-lactam residue analysis in milk using phenyl-column chemistry prior to detection by tandem mass spectrometry.

Anal Bioanal Chem 2020 May 23. Epub 2020 May 23.

Food Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.

Analyte isobaric interferences can limit the development of a comprehensive analytical method for the quantitative liquid chromatography-tandem mass spectrometry profiling of an important cohort of veterinary drugs. In this work, a selective chromatographic separation was developed for the analysis of 32 β-lactam antibiotic residues (12 penicillins, 14 cephalosporins, five carbapenems and faropenem) in milk samples. A range of analytical columns with different stationary phases and mobile phases were evaluated for retention and separation of the β-lactam compounds. Read More

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http://dx.doi.org/10.1007/s00216-020-02688-4DOI Listing

A single-cell identification and capture chip for automatically and rapidly determining hydraulic permeability of cells.

Anal Bioanal Chem 2020 May 21. Epub 2020 May 21.

Center for Biomedical Engineering, University of Science and Technology of China, Hefei, 230027, Anhui, China.

The hydraulic permeability of the lipid bilayer membrane of a single cell, a very important parameter in biological and medical fields, has been attracting increasing attention. To date, methods developed to determine this permeability are either operation-complicated or time-consuming. Therefore, we developed a chip for automatically and rapidly determining the permeability of cells that integrates microfluidics and cell impedance analysis. Read More

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http://dx.doi.org/10.1007/s00216-020-02704-7DOI Listing

Deep eutectic solvent-assisted facile synthesis of copper hydroxide nitrate nanosheets as recyclable enzyme-mimicking colorimetric sensor of biothiols.

Anal Bioanal Chem 2020 May 21. Epub 2020 May 21.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China.

In the quest for alternative products that would conquer natural enzyme drawbacks, enzyme-like nanomaterials with controllable morphology, high catalytic activity, excellent stability, and reusability have gained extensive attention in recent years. Herein, a simple and versatile strategy based on basic deep eutectic solvents was used to create layered copper hydroxide nitrate (Cu(OH)NO) with a well-structured nanosheet-like morphology. The present nanosheets exhibited extraordinary oxidase and peroxidase-like activity. Read More

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http://dx.doi.org/10.1007/s00216-020-02712-7DOI Listing

Portable solid sensor supported in nylon for silver ion determination: testing its liberation as biocide.

Anal Bioanal Chem 2020 May 19. Epub 2020 May 19.

MINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.

This paper reports the fabrication and utility of a new solid sensor, which allows the quantitation of silver ions acting as catalyst at the low micromolar level. The optical sensor was prepared by incorporating both reagents, pyrogallol red (PGR) and 1,10-phenanthroline (Phen), in a nylon membrane. The effect of parameters in determining silver-catalyzed oxidation of PGR by persulfate in the presence of Phen as an activator was studied and optimized for achieving suitable sensitivity. Read More

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http://dx.doi.org/10.1007/s00216-020-02680-yDOI Listing

Influence of nonylphenol from multilayer plastic films on artificial insemination of sows.

Anal Bioanal Chem 2020 May 18. Epub 2020 May 18.

Department of Research and Development, Magapor SL, 50600 Ejea de los Caballeros, Zaragoza, Spain.

Artificial insemination is common practice in mass livestock farming. Recently, it was shown that chemicals leaching from multilayer plastic bags affect the fertility of boars, although common quality tests did not show any impact on the sperm. It is not clear whether this incidence was a single case or whether it could be a systematic problem. Read More

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http://dx.doi.org/10.1007/s00216-020-02698-2DOI Listing

Hg isotopic composition and total Hg mass fraction in NIES Certified Reference Material No. 28 Urban Aerosols.

Anal Bioanal Chem 2020 May 18. Epub 2020 May 18.

Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM, Institut des Sciences Analytiques et de Physicochimie pour l'Environnement et les Matériaux, 2 Avenue Pierre Angot, 64053, Pau Cedex 09, France.

An interlaboratory study on the National Institute for Environmental Studies (NIES) certified reference material (CRM) No. 28 Urban Aerosols, collected on the filters of a central ventilating system in a building in Beijing city center, was performed to obtain informative values of Hg isotopic composition and total Hg (THg) mass fraction. The THg mass fraction was determined by four organizations using atomic absorption spectrometry; it resulted in the mean value of 1. Read More

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http://dx.doi.org/10.1007/s00216-020-02691-9DOI Listing

Nanoelectrochemistry in the study of single-cell signaling.

Anal Bioanal Chem 2020 May 18. Epub 2020 May 18.

Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL, 61801, USA.

Label-free biosensing has been the dream of scientists and biotechnologists as reported by Vollmer and Arnold (Nat Methods 5:591-596, 2008). The ability of examining living cells is crucial to cell biology as noted by Fang (Int J Electrochem 2011:460850, 2011). Chemical measurement with electrodes is label-free and has demonstrated capability of studying living cells. Read More

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http://dx.doi.org/10.1007/s00216-020-02655-zDOI Listing

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample.

Anal Bioanal Chem 2020 May 15. Epub 2020 May 15.

Université Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO), ECBB, Bât 420, 2 Rue du Doyen Georges Poitou, 91400, Orsay, France.

An electrochemical sensor for the individual and the simultaneous detection of dopamine (DA), ascorbic acid (AA), and uric acid (UA) based on redox conjugated "poly(para-phenylene)" (Fc-ac-PPP) bearing ferrocene and carboxylic acid in lateral position has been developed. The electrochemical characterization of the sensor has been studied with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and chronoamperometry (CA). We highlighted that the catalytic activity of the Fc-ac-PPP polymer provided by its redox electrochemical properties and chemical structure allows the electrochemical detection of DA, AA, and UA. Read More

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http://dx.doi.org/10.1007/s00216-020-02686-6DOI Listing

Fluorescence calibration standards made from broadband emitters encapsulated in polymer beads for fluorescence microscopy and flow cytometry.

Anal Bioanal Chem 2020 May 14. Epub 2020 May 14.

BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Str. 11, 12489, Berlin, Germany.

We present here the design and characterization of a set of spectral calibration beads. These calibration beads are intended for the determination and regular control of the spectral characteristics of fluorescence microscopes and other fluorescence measuring devices for the readout of bead-based assays. This set consists of micrometer-sized polymer beads loaded with dyes from the liquid Calibration Kit Spectral Fluorescence Standards developed and certified by BAM for the wavelength-dependent determination of the spectral responsivity of fluorescence measuring devices like spectrofluorometers. Read More

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http://dx.doi.org/10.1007/s00216-020-02664-yDOI Listing

Bioanalytics and higher order electrokinetics.

Anal Bioanal Chem 2020 Jun;412(16):3799-3800

Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Via del Politecnico 1, 00133, Rome, Italy.

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http://dx.doi.org/10.1007/s00216-020-02694-6DOI Listing

Accurate estimation of diffusion coefficient for molecular identification in a complex background.

Anal Bioanal Chem 2020 May 14. Epub 2020 May 14.

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China.

To eliminate the effects of complex background signals and to enhance the accuracy of the diffusion coefficient measurement, derivative NMR spectroscopy with negligible loss of the spectral quality is introduced based on the customized Savitzky-Golay method and used to construct diffusion-ordered NMR spectroscopy (DOSY). The criterion of the method was established by simulations. The application of this method on mouse urine and serum showed that the accuracy and precision of diffusion coefficient measurements in a complex background were improved to enhance the identification of molecules. Read More

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http://dx.doi.org/10.1007/s00216-020-02693-7DOI Listing

Challenges in the analytical determination of ultra-short-chain perfluoroalkyl acids and implications for environmental and human health.

Anal Bioanal Chem 2020 May 12. Epub 2020 May 12.

Man-Technology-Environment Research Centre (MTM), Örebro University, 701 82, Örebro, Sweden.

Ultra-short-chain perfluoroalkyl acids have recently gained attention due to increasing environmental concentrations being observed. The most well-known ultra-short-chain perfluoroalkyl acid is trifluoroacetic acid (TFA) which has been studied since the 1990s. Potential sources and the fate of ultra-short-chain perfluoroalkyl acids other than TFA are not well studied and data reporting their environmental occurrence is scarce. Read More

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http://dx.doi.org/10.1007/s00216-020-02692-8DOI Listing

Smartphone-interrogated test supports for the enzymatic determination of putrescine and cadaverine in food.

Anal Bioanal Chem 2020 May 12. Epub 2020 May 12.

Nanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Aragon Institute of Nanoscience, University of Zaragoza, 50009, Zaragoza, Spain.

Diamino-oxidase (DAO), horseradish peroxidase (HRP), and tetramethylbenzidine (TMB) have been immobilized into cellulose to obtain circular cellulose test supports (CCTSs) for the determination of cadaverine (Cad) and putrescine (Put). During the enzymatic reaction, TMB is oxidized and a blue spot is obtained. This color (RGB coordinates) is measured with a smartphone and a commercial application. Read More

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http://dx.doi.org/10.1007/s00216-020-02677-7DOI Listing

Determination of halogens and sulfur in honey: a green analytical method using a single analysis.

Anal Bioanal Chem 2020 May 12. Epub 2020 May 12.

Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Capão do Leão, RS, 96160-000, Brazil.

The halogen determination is important in view of their biological and environmental roles, but their determination has still been considered a challenge, especially at low concentrations. Therefore, a method for honey decomposition using microwave-induced combustion (MIC) combined with ion chromatography and conductimetric detection (for Cl, F, and S determination) or mass detection (for Br and I determination) (IC-CD-MS) is proposed. Trueness was evaluated by adding reference materials (RMs) or a standard solution in the sample. Read More

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http://dx.doi.org/10.1007/s00216-020-02636-2DOI Listing

Myoglobin-silver reduced graphene oxide nanocomposite stochastic biosensor for the determination of luteinizing hormone and follicle-stimulating hormone from saliva samples.

Anal Bioanal Chem 2020 May 12. Epub 2020 May 12.

National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, 400293, Cluj-Napoca, Romania.

A silver reduced graphene oxide (Ag-rGO) nanocomposite paste was prepared for the assay of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children's saliva. The paste was modified with a solution of myoglobin (Myb) to form the stochastic biosensor, to improve the sensitivity of the method. The Ag-rGO powder and Myb-Ag-rGO paste were morphologically and structurally characterized by SEM, TEM and XRD measurements. Read More

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http://dx.doi.org/10.1007/s00216-020-02663-zDOI Listing

Preparation of SnS/MWCNTs chemically modified electrode and its electrochemical detection of HO.

Anal Bioanal Chem 2020 May 12. Epub 2020 May 12.

Key Laboratory of Advanced Technologies of Materials (Ministry of Education of China), Superconductivity and New Energy R&D Centre, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.

Considering the importance of hydrogen peroxide (HO) rapid detection, a SnS/MWCNTs composite was prepared by loading tin disulfide (SnS) nanoparticles on a three-dimensional conductive network composed of multi-walled carbon nanotubes (MWCNTs). The obtained SnS/MWCNTs composite was used as the modified material to prepare a chemically modified electrode (CME), which was used for the rapid detection of HO. The morphology and structure of the obtained samples were characterized and analyzed by scanning electron microscopy, X-ray diffraction, and energy-dispersive spectroscopy. Read More

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http://dx.doi.org/10.1007/s00216-020-02682-wDOI Listing

Hanging drop cathode-atmospheric pressure glow discharge as a new method of sample introduction for inductively coupled plasma-optical emission spectrometry.

Anal Bioanal Chem 2020 May 11. Epub 2020 May 11.

Faculty of Chemistry, Department of Analytical Chemistry and Chemical Metallurgy, Wroclaw University of Science and Technology, Wybrzeze Stanislawa Wyspianskiego 27, 50-370, Wroclaw, Poland.

This work reports the use of hanging drop cathode-atmospheric pressure glow discharge (HDC-APGD) as a new method of sample introduction for inductively coupled plasma-optical emission spectrometry (ICP-OES). The developed arrangement was characterized by a low sample uptake (0.56 mL min) and the fact that the entire sample solution volume was consumed by the discharge. Read More

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http://dx.doi.org/10.1007/s00216-020-02685-7DOI Listing
May 2020
3.436 Impact Factor

Molecularly imprinted polyaniline for detection of horseradish peroxidase.

Anal Bioanal Chem 2020 May 9. Epub 2020 May 9.

Institute of Chemistry, Saratov State University, Astrakhanskaya 83, Saratov, Russia, 410012.

A new facile and fast approach to the synthesis of polyaniline (PANi) molecularly imprinted polymers (MIPs) based on aniline oxidative chemical polymerization was proposed for protein recognition. For the first time, a surface imprinting strategy was implemented for the synthesis of PANi MIPs on the inner surface of soft glass polycapillaries (PC) with a large (2237) number of individual microcapillaries. Two different PANi layers-(i) PANi film and (ii) protein imprinted PANi nanowires-were synthesized sequentially. Read More

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http://dx.doi.org/10.1007/s00216-020-02689-3DOI Listing

Artificial neural networks for quantitative online NMR spectroscopy.

Anal Bioanal Chem 2020 May 9. Epub 2020 May 9.

Bundesanstalt für Materialforschung und -prüfung (BAM), Unter den Eichen 44-46, 12203, Berlin, Germany.

Industry 4.0 is all about interconnectivity, sensor-enhanced process control, and data-driven systems. Process analytical technology (PAT) such as online nuclear magnetic resonance (NMR) spectroscopy is gaining in importance, as it increasingly contributes to automation and digitalization in production. Read More

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http://dx.doi.org/10.1007/s00216-020-02687-5DOI Listing

The role of surface in desorption electrospray ionization-mass spectrometry: advances and future trends.

Anal Bioanal Chem 2020 May 9. Epub 2020 May 9.

Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124, Parma, Italy.

An outlook on the current status and trends in desorption electrospray ionization-mass spectrometry (DESI-MS), one of the most common spray-based techniques for ambient ionization, is given with a focus on the main advances recently achieved or still in progress regarding studies of surface properties affecting the signal stability and efficiency of the DESI process. Future directions that the field may take in the years to come are discussed, with particular focus on bioanalytical research. Read More

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http://dx.doi.org/10.1007/s00216-020-02671-zDOI Listing

Advances in direct optical detection.

Anal Bioanal Chem 2020 May;412(14):3263-3264

Institute of Photonics and Electronics of the Czech Academy of Sciences, Chaberska 57, 18251, Prague 8, Czech Republic.

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http://dx.doi.org/10.1007/s00216-020-02617-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205597PMC

Infrared spectroscopy as a tool to monitor interactions between nanoplastics and microalgae.

Anal Bioanal Chem 2020 May 8. Epub 2020 May 8.

Le Mans Université, IMMM UMR-CNRS 6283, Avenue Olivier Messiaen, 72085, Le Mans Cedex 9, France.

The unicellular photosynthetic organisms known as microalgae are becoming one of the most important models for aquatic system studies. Among them, Chlamydomonas reinhardtii is widely used as a bioindicator of pollution or of different changes in the environment. Numerous pollutants are present in aquatic environments, particularly plastics and nanoplastics. Read More

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http://dx.doi.org/10.1007/s00216-020-02683-9DOI Listing

Source-related smart suspect screening in the aqueous environment: search for tire-derived persistent and mobile trace organic contaminants in surface waters.

Anal Bioanal Chem 2020 May 8. Epub 2020 May 8.

Department of Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany.

A variant of suspect screening by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) is proposed in this study: Samples of a potential source of contamination and of an environmental sample close to this source are first analyzed in a non-targeted manner to select source-related suspects and to identify them. The suspect list compiled from such an exercise is then applied to LC-HRMS data of environmental samples to ascribe and to identify persistent and mobile contaminants in the water cycle that may originate from the source under study. This approach was applied to tire crumb rubber (source) and road dust (close to source); by comparison of the two data sets, 88% of the features detected in tire leachate could be excluded. Read More

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http://dx.doi.org/10.1007/s00216-020-02653-1DOI Listing

Optical sensors for determination of biogenic amines in food.

Anal Bioanal Chem 2020 May 8. Epub 2020 May 8.

Institute of Analytical Chemistry, Chemo and Biosensors, University of Regensburg, 93040, Regensburg, Germany.

This review presents the state-of-the-art of optical sensors for determination of biogenic amines (BAs) in food by publications covering about the last 10 years. Interest in the development of rapid and preferably on-site methods for quantification of BAs is based on their important role in implementation and regulation of various physiological processes. At the same time, BAs can develop in different kinds of food by fermentation processes or microbial activity or arise due to contamination, which induces toxicological risks and food poisoning and causes serious health issues. Read More

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http://dx.doi.org/10.1007/s00216-020-02675-9DOI Listing

CRM rapid response approach for the certification of arsenic species and toxic trace elements in baby cereal coarse rice flour certified reference material BARI-1.

Anal Bioanal Chem 2020 May 7. Epub 2020 May 7.

National Research Council Canada, 1200 Montreal Rd, Ottawa, ON, K1A 0R6, Canada.

With recently legislated maximum levels of inorganic arsenic (iAs) in white and brown rice in Canada, the regulatory bodies are evaluating the need for regulation of As levels in infant food products. Rice is a major part of infants' diet, and therefore, the presence of As in this staple food causes concerns. So far, the scientific community was lacking suitable certified reference material (CRM) which could be used to assess the accuracy of developed analytical methods for As speciation in infants' food products. Read More

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http://dx.doi.org/10.1007/s00216-020-02673-xDOI Listing

Analysis of the human chorionic gonadotropin protein at the intact level by HILIC-MS and comparison with RPLC-MS.

Anal Bioanal Chem 2020 May 6. Epub 2020 May 6.

Laboratory of Analytical, Bioanalytical Sciences and Miniaturization, UMR CBI 8231 ESPCI Paris - CNRS, PSL University, 75005, Paris, France.

In the present work, the human chorionic gonadotropin (hCG) hormone was characterized for the first time by hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution (HR) quadrupole/time-of-flight (qTOF) mass spectrometry (MS) at the intact level. This heterodimeric protein, consisting of two subunits (hCGα and hCGβ), possesses 8 potential glycosylation sites leading to a high number of glycoforms and has a molecular weight of about 35 kDa. The HILIC conditions optimized in a first paper but using UV detection were applied here with MS for the analysis of two hCG-based drugs, a recombinant hCG and a hCG isolated from the urine of pregnant women. Read More

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http://dx.doi.org/10.1007/s00216-020-02684-8DOI Listing

Polyethylene glycol crowding effect on hyaluronidase activity monitored by capillary electrophoresis.

Anal Bioanal Chem 2020 May 6. Epub 2020 May 6.

Institut de Chimie Organique et Analytique (ICOA), CNRS FR 2708 - UMR 7311, Université d'Orléans, 45067, Orléans, France.

To mimic the activity of hyaluronidase in natural environment, the hydrolysis of hyaluronic acid (HA) by hyaluronidase was investigated for the first time in the presence of crowding agents using capillary electrophoresis (CE) as a simple and reliable technique for conducting enzymatic assay. Polyethylene glycol (PEG) 6000 was selected as a model crowder and the hyaluronic acid degradation catalyzed by bovine testes hyaluronidase (BTH) was carried out at different PEG concentrations (0%, 10%, and 17%). After optimization of the CE analytical method and enzymatic assay, the degradation products were monitored at different HA concentrations. Read More

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http://dx.doi.org/10.1007/s00216-020-02659-9DOI Listing
May 2020
3.436 Impact Factor

Determination of citalopram in fish brain tissue: benefits of coupling laser diode thermal desorption with low- and high-resolution mass spectrometry.

Anal Bioanal Chem 2020 May 6. Epub 2020 May 6.

Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic.

Recent state-of-the-art methods developed for the analysis of polar xenobiotics from different types of biological matrices usually employ liquid chromatography with mass spectrometry. However, there are limitations when a small amount of sample mass is available. For example, individual benthic invertebrates or fish tissue samples often weigh less than 100 mg (e. Read More

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http://dx.doi.org/10.1007/s00216-020-02672-yDOI Listing

Trends using biological target-based assays for drug detection in complex sample matrices.

Anal Bioanal Chem 2020 May 6. Epub 2020 May 6.

Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration (FDA), 8301 Muirkirk Rd, Laurel, MD, 20708, USA.

In vivo, drug molecules interact with their biological targets (e.g., enzymes, receptors, ion channels, transporters), thereby eliciting therapeutic effects. Read More

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http://dx.doi.org/10.1007/s00216-020-02681-xDOI Listing

Quantitative analysis of proanthocyanidins in cocoa using cysteamine-induced thiolysis and reversed-phase UPLC.

Anal Bioanal Chem 2020 May 5. Epub 2020 May 5.

Methods and Application of Food Composition Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD, 20705, USA.

The thiolysis of B-type proanthocyanidins in cocoa by cysteamine was evaluated and optimized for its application in cocoa proanthocyanidin quantification. Four thiolysis products consisting of epicatechin, catechin, and their thioethers formed with cysteamine were separated and characterized by reversed-phase UPLC with photo diode array (PDA) detection and high-resolution mass spectrometry (HRMS). A thiolysis time of 20 min under 60 °C temperature was determined as the optimal condition for cocoa proanthocyanidin depolymerization. Read More

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http://dx.doi.org/10.1007/s00216-020-02669-7DOI Listing

Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer.

Anal Bioanal Chem 2020 Jun 5;412(16):3881-3889. Epub 2020 May 5.

Department of Electrical and Computer Engineering, University of Virginia, Charlottesville, VA, 22904, USA.

The trapping and deflection of biological cells by dielectrophoresis (DEP) at field non-uniformities in a microfluidic device is often conducted in a contactless dielectrophoresis (cDEP) mode, wherein the electrode channel is in a different layer than the sample channel, so that field penetration through the interceding barrier causes DEP above critical cut-off frequencies. In this manner, through physical separation of the electrode and sample channels, it is possible to spatially modulate electric fields with no electrode-induced damage to biological cells in the sample channel. However, since this device requires interlayer alignment of the electrode to sample channel and needs to maintain a thin interceding barrier (~ 15 μm) over the entire length over which DEP is needed (~ 1 cm), variations in alignment and microstructure fidelity cause wide variations in cDEP trapping level and frequency response across devices. Read More

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http://dx.doi.org/10.1007/s00216-020-02667-9DOI Listing

Imaging mass spectrometry to visualise increased acetylcholine in lungs of asthma model mice.

Anal Bioanal Chem 2020 May 4. Epub 2020 May 4.

Department of Optical Imaging, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.

Acetylcholine (ACh) is a crucial neurotransmitter that is involved in airway constriction. In fact, excessive ACh binding to M3 muscarinic receptor leads to airflow obstruction via smooth muscle contraction. Previous studies have suggested cholinergic malfunction in the pathogenesis of asthma; however, the distribution and abundance of ACh in asthmatic lungs remain unclear because of the challenges of imaging ACh in lung tissue. Read More

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http://dx.doi.org/10.1007/s00216-020-02670-0DOI Listing

Unveiling the molecular mechanisms underpinning biorecognition of early-glycated human serum albumin and receptor for advanced glycation end products.

Anal Bioanal Chem 2020 May 4. Epub 2020 May 4.

Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy.

Serum levels of early-glycated albumin are significantly increased in patients with diabetes mellitus and may play a role in worsening inflammatory status and sustaining diabetes-related complications. To investigate possible pathological recognition involving early-glycated albumin and the receptor for advanced glycation end products (RAGE), an early-glycated human serum albumin (HSAgly), with a glycation pattern representative of the glycated HSA form abundant in diabetic patients, and the recombinant human RAGE ectodomain (VC1) were used. Biorecognition between the two interactants was investigated by combining surface plasmon resonance (SPR) analysis and affinity chromatography coupled with mass spectrometry (affinity-MS) for peptide extraction and identification. Read More

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http://dx.doi.org/10.1007/s00216-020-02674-wDOI Listing

Real-time monitoring of extracellular pH using a pH-potentiometric sensing SECM dual-microelectrode.

Anal Bioanal Chem 2020 Jun 3;412(15):3737-3743. Epub 2020 May 3.

School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.

Extracellular pH can indicate the variation in organelle function and cell state. It is important to measure extracellular pH (pHe) with a controllable distance. In this work, a potentiometric SECM dual-microelectrode was developed to monitor the pHe of MCF-7 cells under electrical stimulation. Read More

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http://dx.doi.org/10.1007/s00216-020-02625-5DOI Listing
June 2020
3.436 Impact Factor

Evaluation of the quantity and the carbon isotopic composition of amino acids by using diverse sample residues after lipid extraction.

Anal Bioanal Chem 2020 May 3. Epub 2020 May 3.

Department of Marine Science and Convergent Engineering, Hanyang University, Ansan, 15588, Republic of Korea.

Compound-specific stable isotope analysis (CSIA) becomes a critical tool in the fields of ecology and biogeochemistry to trace basal carbon sources such as amino acids (AAs) and fatty acids (FAs) in natural environments. However, in many studies, either AAs or FAs have been analyzed due to restricted sample amounts. The aim of this study is to report a single-sample preparation method by identifying AAs using lipid-free residues derived from a typical FA analytic procedure. Read More

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http://dx.doi.org/10.1007/s00216-020-02679-5DOI Listing

Development of a thin-film solid-phase microextraction (TF-SPME) method coupled to liquid chromatography and tandem mass spectrometry for high-throughput determination of steroid hormones in white sucker fish plasma.

Anal Bioanal Chem 2020 May 2. Epub 2020 May 2.

Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.

Steroid hormones (SH) play a number of important physiological roles in vertebrates including fish. Changes in SH concentration significantly affect reproduction, differentiation, development, or metabolism. The objective of this study was to develop an in vitro high-throughput thin-film solid-phase microextraction (TF-SPME)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for targeted analysis of endogenous SH (cortisol, testosterone, progesterone, estrone (E1), 17β-estradiol (E2), and 17α-ethinylestradiol (EE2)) in wild white sucker fish plasma where the concentrations of the analytes are substantially low. Read More

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http://dx.doi.org/10.1007/s00216-020-02657-xDOI Listing

Ratiometric assay of mercury ion based on nitrogen-doped carbon dots with two different optical signals: second-order scattering and fluorescence.

Anal Bioanal Chem 2020 May 1. Epub 2020 May 1.

Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.

Ratiometric assays, which can effectively surmount external interference, have attracted extensive research interests. Herein, a novel ratiometric sensing platform for Hg is designed based on nitrogen-doped carbon dots (N-CDs) with two different optical signals. Under a single excitation, N-CDs have two emission peaks around 668 nm and 412 nm, which are second-order scattering and fluorescence, respectively. Read More

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http://dx.doi.org/10.1007/s00216-020-02676-8DOI Listing

Application of surface-imprinted polymers supported by hydroxyapatite in the extraction of zearalenone in various cereals.

Anal Bioanal Chem 2020 May 1. Epub 2020 May 1.

College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.

Surface-imprinted polymers supported by hydroxyapatite (HAP@MIPs) were prepared using coumarin-3-carboxylic acid and naringenin as dummy template molecules of zearalenone (ZEA). HAP@MIPs were characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, particle size distribution analysis, energy-dispersive X-ray spectroscopy, and X-ray diffraction. The adsorption performance was studied. Read More

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http://dx.doi.org/10.1007/s00216-020-02610-yDOI Listing

Tb-doped BSA-gold nanoclusters as a bimodal probe for the selective detection of TNT.

Anal Bioanal Chem 2020 May 1. Epub 2020 May 1.

Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, 695581, India.

Trinitrotoluene (TNT) is a widely used explosive belonging to the family of nitroaromatic compounds, and its misuse poses a significant threat to society. Herein, we propose a Tb-BSA-AuNC fluorescent and colorimetric sensing probe for the selective onsite detection of TNT in the aqueous phase. Tb-doped BSA-protected gold nanoclusters (Tb-BSA-AuNCs) were synthesized by a microwave-assisted method, and TNT detection was carried out utilizing the chemistry of Meisenheimer complex formation. Read More

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http://dx.doi.org/10.1007/s00216-020-02654-0DOI Listing

Application of chemometric methods to the analysis of multimodal chemical images of biological tissues.

Anal Bioanal Chem 2020 Apr 30. Epub 2020 Apr 30.

Institute of Environmental Assessment and Water Research (IDAEA-CSIC), 08034, Barcelona, Spain.

Current histology techniques, such as tissue staining or histochemistry protocols, provide very limited chemical information about the tissues. Chemical imaging technologies such as infrared, Raman, and mass spectrometry imaging, are powerful analytical techniques with a huge potential in describing the chemical composition of sample surfaces. In this work, three images of the same tissue slice using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, infrared microspectroscopy, and an RGB picture from a conventional hematoxylin/eosin (H/E) staining are simultaneously analyzed. Read More

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http://dx.doi.org/10.1007/s00216-020-02595-8DOI Listing

Analytical methods for mixed organic chemical residues and contaminants in food.

Anal Bioanal Chem 2020 Apr 30. Epub 2020 Apr 30.

Center for Veterinary Medicine, Office of Research, US Food and Drug Administration, Laurel, MD, 20708, USA.

Developing methods that can analyze multiple categories of organic chemical residues such as pesticides, veterinary drugs, mycotoxins, human drugs, and environmental contaminants in food with a single analytical procedure is a growing trend. These methods for mixed organic chemical residues and contaminants focus on the chemical properties of these analytes rather than how they are used and adulterate the food supply. This paper highlights recently published methods for mixed residue and contaminant methods in food including advances in technology (instrumental hardware, data processing programs, and sample cleanup) that allow for a larger number of compounds to be monitored simultaneously. Read More

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http://dx.doi.org/10.1007/s00216-020-02668-8DOI Listing

Discrimination of grass pollen of different species by FTIR spectroscopy of individual pollen grains.

Anal Bioanal Chem 2020 Apr 29. Epub 2020 Apr 29.

Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany.

Fourier-transform infrared (FTIR) spectroscopy enables the chemical characterization and identification of pollen samples, leading to a wide range of applications, such as paleoecology and allergology. This is of particular interest in the identification of grass (Poaceae) species since they have pollen grains of very similar morphology. Unfortunately, the correct identification of FTIR microspectroscopy spectra of single pollen grains is hindered by strong spectral contributions from Mie scattering. Read More

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http://dx.doi.org/10.1007/s00216-020-02628-2DOI Listing

Identification of adjuvants in plant protection products applying a suspect screening workflow based on orthogonal techniques.

Anal Bioanal Chem 2020 Apr 29. Epub 2020 Apr 29.

Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), University of Almeria, Agrifood Campus of International Excellence, ceiA3, 04120, Almeria, Spain.

In this study, the identification of adjuvants (surfactants and solvents) in quizalofop-p-ethyl plant protection products (PPPs) was carried out by applying nuclear magnetic resonance (NMR), ultra-high-performance liquid chromatography (UHPLC) and gas chromatography (GC), both coupled to high-resolution mass spectrometry (HRMS), and using a suspect analysis approach. NMR provided a rapid and global overview of the composition of the studied samples and supported the tentative identification of the glycol ether family, commonly employed as surfactants, and 2-ethyl-1-butanol. UHPLC-HRMS was used for characterization of the glycol polymer surfactants, while GC-HRMS was used to obtain information about volatile organic compounds (benzene or naphthalene derivates) present in the PPPs. Read More

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http://dx.doi.org/10.1007/s00216-020-02662-0DOI Listing

Identification of adulteration in botanical samples with untargeted metabolomics.

Anal Bioanal Chem 2020 Apr 29. Epub 2020 Apr 29.

Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.

Adulteration remains an issue in the dietary supplement industry, including botanical supplements. While it is common to employ a targeted analysis to detect known adulterants, this is difficult when little is known about the sample set. With this study, untargeted metabolomics using liquid chromatography coupled to ultraviolet-visible spectroscopy (LC-UV) or high-resolution mass spectrometry (LC-MS) was employed to detect adulteration in botanical dietary supplements. Read More

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http://dx.doi.org/10.1007/s00216-020-02678-6DOI Listing

Double molecular recognition strategy based on boronic acid-diol and NHS ester-amine for selective electrochemical detection of cerebral dopamine.

Anal Bioanal Chem 2020 Jun 29;412(15):3727-3736. Epub 2020 Apr 29.

Department of Anesthesiology, The First People's Hospital of Foshan, Foshan, 528000, Guangdong, China.

Electrochemical detection of dopamine (DA) usually depends on electrochemical oxidation of DA. This conventional method can hardly provide sufficient sensitivity and selectivity in the determination of the cerebral DA down to nanomolar level, because the ability of DA to be electrochemically oxidized is limited and many electroactive interferents are also oxidized at a similar potential with DA. Here, an electrochemical assay based on a double molecular recognition strategy has been proposed and proved to be of high sensitivity and selectivity for DA measurement in the cerebral system. Read More

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http://dx.doi.org/10.1007/s00216-020-02624-6DOI Listing