13,239 results match your criteria Analytical and bioanalytical chemistry[Journal]


ABC Spotlight on Analytics 4.0.

Anal Bioanal Chem 2018 Jun 23. Epub 2018 Jun 23.

University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, Universitätsstrasse 31, 93053, Regensburg, Germany.

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An ultrasensitive and simple method for alkaline phosphatase assay and targeted natural compound screening in vitro.

Anal Bioanal Chem 2018 Jun 22. Epub 2018 Jun 22.

TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Pakistan TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China.

As an essential phosphate group hydrolase, alkaline phosphatase (ALP), whose level in serum is correlated with bone disease, liver dysfunction, and cancer, could be used as a biomarker for clinical diagnosis and biomedical studies. Hence, developing a convenient and sensitive method for ALP assay has importance in disease diagnosis, drug treatment, and prognosis assessment. In this work, using a hairpin DNA strand as the substrate, we developed an ultrasensitive and simple fluorescence method for quantitative ALP assay based on the binding difference of reduced graphene oxide (rGO) with different DNA strands coupled with λ exonuclease (λ exo) cleavage. Read More

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A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters.

Anal Bioanal Chem 2018 Jun 22. Epub 2018 Jun 22.

Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, 16th Azar St., Enghelab Sq, P.O. Box 14155-6619, Tehran, Iran.

DNA methylation catalyzed by DNA methyl transferase (MTase) is a significant epigenetic process for modulating gene expression. Abnormal levels of DNA MTase enzyme have been regarded as a cancer biomarker or a sign of bacterial diseases. We developed a novel colorimetric method to assay M. Read More

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Through-packaging analysis of butter adulteration using line-scan spatially offset Raman spectroscopy.

Anal Bioanal Chem 2018 Jun 22. Epub 2018 Jun 22.

Department of Biosystems Machinery Engineering, College of Agricultural and Life Science, Chungnam National University, 99 Daehak-ro, Yuseoung-gu, Daejeon, 34134, South Korea.

Spectroscopic techniques for food quality analysis are limited to surface inspections and are highly affected by the superficial layers (skin or packaging material) of the food samples. The ability of spatially offset Raman spectroscopy (SORS) to obtain chemical information from below the surface of a sample makes it a promising candidate for the non-destructive analysis of the quality of packaged food. In the present study, we developed a line-scan SORS technique for obtaining the Raman spectra of packaged-food samples. Read More

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A many probes-one spot hybridization oligonucleotide microarray.

Anal Bioanal Chem 2018 Jun 22. Epub 2018 Jun 22.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 8, Novosibirsk, 630090, Russia.

A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses (Variola, Monkeypox, and Ectromelia viruses) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. Read More

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June 2018
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Development of analytical methods for evaluating the quality of dissociated and associated amphiphilic poly(γ-glutamic acid) nanoparticles.

Anal Bioanal Chem 2018 Jun 22. Epub 2018 Jun 22.

Building Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, 565-0871, Japan.

A quantitative method of analyzing nanoparticles (NPs) for drug delivery is urgently required by researchers and industry. Therefore, we developed new quantitative analytical methods for biodegradable and amphiphilic NPs consisting of polymeric γ-PGA-Phe [phenylalanine attached to poly(γ-glutamic acid)] molecules. These γ-PGA-Phe NPs were completely dissociated into separate γ-PGA-Phe molecules by adding sodium dodecyl sulfate (SDS). Read More

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June 2018
1 Read

Vitamin B3 mystery challenge.

Authors:
Lucia D'Ulivo

Anal Bioanal Chem 2018 Jul;410(17):3925-3926

, Ottawa, Canada.

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Solution to collision induced dissociation mass spectrometry challenge.

Anal Bioanal Chem 2018 Jul;410(17):3927-3930

Department of Chemistry, University of Guelph, Guelph, ON, Canada.

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Tools for detecting insect semiochemicals: a review.

Anal Bioanal Chem 2018 Jul 11;410(17):4091-4108. Epub 2018 Jun 11.

Embrapa Genetic Resources and Biotechnology-PqEB-W5, Asa Norte, Brasília, DF, 70770-917, Brazil.

Semiochemicals are chemical compounds that are released by many species as a means of intra- and interspecific communication. Insects have extremely advanced olfactory systems; indeed, they rely on smell when performing many of their main behaviors, such as oviposition, breeding, prey location, and defense. This characteristic of insects implies that semiochemicals could be used for various applications, including in agriculture, where they could be employed along with other tools to control pest insects. Read More

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Eric Lagally (Ed.): Microfluidics and nanotechnology: biosensing to the single molecule limit.

Anal Bioanal Chem 2018 Jun 20. Epub 2018 Jun 20.

Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, 36849, USA.

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A molecularly imprinted sensor with enzymatic enhancement of electrochemiluminescence of quantum dots for ultratrace clopyralid determination.

Anal Bioanal Chem 2018 Jun 19. Epub 2018 Jun 19.

College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, Guangxi, China.

A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. Read More

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Search of non-ionic surfactants suitable for micellar liquid chromatography.

Anal Bioanal Chem 2018 Jun 19. Epub 2018 Jun 19.

Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.

Most reports in reversed-phase liquid chromatography (RPLC) with micellar mobile phases make use of the anionic sodium dodecyl sulfate. This surfactant masks efficiently the silanol groups that are the origin of the poor efficiencies and tailing peaks observed for basic compounds in conventional RPLC. However, it has the handicap of yielding excessive retention, which forces the addition of an organic solvent to reduce the retention times to practical values. Read More

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June 2018
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Cross-reactivity by botanicals used in dietary supplements and spices using the multiplex xMAP food allergen detection assay (xMAP FADA).

Anal Bioanal Chem 2018 Jun 18. Epub 2018 Jun 18.

Office of Regulatory Science, Center for Food Safety and Applied Nutrition (CFSAN), Food and Drug Administration, 5001 Campus Drive, College Park, MD, 20740, USA.

Food allergies affect some 15 million Americans. The only treatment for food allergies is a strict avoidance diet. To help ensure the reliability of food labels, analytical methods are employed; the most common being enzyme-linked immunosorbent assays (ELISAs). Read More

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Electrochemical nonenzymatic sensor for cholesterol determination in food.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Physical and Analytical Chemistry Department, Institute of Natural Resources, Tomsk Polytechnic University, Lenin av. 30, 634050, Tomsk, Russia.

The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Read More

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What about the herb? A new metabolomics approach for synthetic cannabinoid drug testing.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Research Institute for Pesticides and Water, University Jaume I, Avda Sos Baynat s/n, 12071, Castellón, Spain.

Synthetic cannabinoids (SCs) are consumed as legal alternative to cannabis and often allow passing drug-screening tests. Their rapid transience on the drug scene, combined with their mostly unknown metabolic profiles, creates a scenario with constantly moving analytical targets, making their monitoring and identification challenging. The development of fast screening strategies for SCs, not directly focused on their chemical structure, as an alternative to the commonly applied target acquisition methods, would be highly appreciated in forensic and public health laboratories. Read More

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In vivo tumor imaging by a γ-glutamyl transpeptidase-activatable near-infrared fluorescent probe.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

γ-Glutamyl transpeptidase (GGT), overexpressed in various cancer cells, has been perceived as a latent tumor biomarker. Thus, developing near-infrared (NIR) fluorescent GGT probes is highly desired for in vivo tumor imaging and studies. To our knowledge, however, such a GGT probe is still rare. Read More

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Construction of a thermoresponsive magnetic porous polymer membrane enzyme reactor for glutaminase kinetics study.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Key Laboratory of Analytical Chemistry for Living Biosystems, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing, 100190, China.

Fabrication of polymer membranes with nanopores and a confinement effect toward enzyme immobilization has been an enabling endeavor. In the work reported here, an enzyme reactor based on a thermoresponsive magnetic porous block copolymer membrane was designed and constructed. Reversible addition-fragmentation chain transfer polymerization was used to synthesize the block copolymer, poly(maleic anhydride-styrene-N-isopropylacrylamide), with poly(N-isopropylacrylamide) as the thermoresponsive moiety. Read More

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Comparison of μ-ATR-FTIR spectroscopy and py-GCMS as identification tools for microplastic particles and fibers isolated from river sediments.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Leibniz-Institut für Polymerforschung Dresden e.V. (IPF), Hohe Str. 6, 01069, Dresden, Germany.

In recent years, many studies on the analysis of microplastics (MP) in environmental samples have been published. These studies are hardly comparable due to different sampling, sample preparation, as well as identification and quantification techniques. Here, MP identification is one of the crucial pitfalls. Read More

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Analysis of human C24 bile acids metabolome in serum and urine based on enzyme digestion of conjugated bile acids and LC-MS determination of unconjugated bile acids.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Metabolomics Shared Resource, University of Hawaii Cancer Center, Honolulu, HI, 96801, USA.

Host-gut microbiota metabolic interactions are closely associated with health and disease. A manifestation of such co-metabolism is the vast structural diversity of bile acids (BAs) involving both oxidative stereochemistry and conjugation. Herein, we describe the development and validation of a LC-MS-based method for the analysis of human C24 BA metabolome in serum and urine. Read More

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June 2018
2 Reads

Strain-level typing and identification of bacteria - a novel approach for SERS active plasmonic nanostructures.

Anal Bioanal Chem 2018 Jun 16. Epub 2018 Jun 16.

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.

One of the potential applications of surface-enhanced Raman spectroscopy (SERS) is the detection of biological compounds and microorganisms. Here we demonstrate that SERS coupled with principal component analysis (PCA) serves as a perfect method for determining the taxonomic affiliation of bacteria at the strain level. We demonstrate for the first time that it is possible to distinguish different genoserogroups within a single species, Listeria monocytogenes, which is one of the most virulent foodborne pathogens and in some cases contact with which may be fatal. Read More

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Colorimetric determination of glutathione in human serum and cell lines by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite.

Anal Bioanal Chem 2018 Jun 7. Epub 2018 Jun 7.

College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an, 625014, Sichuan, China.

In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Read More

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Molecular switch-modulated fluorescent copper nanoclusters for selective and sensitive detection of histidine and cysteine.

Anal Bioanal Chem 2018 Jun 7. Epub 2018 Jun 7.

School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.

A novel assay for histidine and cysteine has been constructed based on modulation of fluorescent copper nanoclusters (CuNCs) by molecular switches. In our previous work, a dumbbell DNA template with a poly-T (thymine) loop has been developed as an excellent template for the formation of strongly fluorescent CuNCs. Herein, for the first time, we established this biosensor for sensing two amino acids by using dumbbell DNA-templated CuNCs as the single probe. Read More

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Enhanced molecular recognition for imprinted monolithic column containing polyhedral oligomeric silsesquioxanes by dendritic effect of mesoporous molecular sieve scaffolds.

Anal Bioanal Chem 2018 Jun 7. Epub 2018 Jun 7.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.

The dendritic effect of nano mesoporous molecular sieve was first used to enhance molecular recognition of molecularly imprinted polymers (MIPs)-based polyhedral oligomeric silsesquioxanes (POSS). In this study, the MIPs were made using S-naproxen (S-NAP) as template molecule, 4-vinylpyridine (4-VP) as functional monomer, ethylene glycol dimethacrylate as cross-linker, 1-butyl-3-methylimidazoliumtetrafluoroborate ([BMIM]BF)/DMSO as binary porogens, 1-propylmethacrylate-heptaisobutyl substituted as POSS monomer, and mesoporous molecular sieve (Mobil composition of matter No. 41, MCM-41) as dendritic scaffold. Read More

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June 2018
2 Reads

Combination of H nuclear magnetic resonance spectroscopy and principal component analysis to evaluate the lipid fluidity of flutamide-encapsulated lipid nanoemulsions.

Anal Bioanal Chem 2018 Jun 6. Epub 2018 Jun 6.

Department of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Nakauchicho, Misasagi, Yamashina-ku, Kyoto, 607-8414, Japan.

The lipid fluidity of various lipid nanoemulsions (LNEs) without and with flutamide (FT) and containing one of two neutral lipids, one of four phosphatidylcholines as a surfactant, and sodium palmitate as a cosurfactant was investigated by the combination of H nuclear magnetic resonance (NMR) spectroscopy and principal component analysis (PCA). In the H NMR spectra, the peaks from the methylene groups of the neutral lipids and surfactants for all LNE preparations showed downfield shifts with increasing temperature from 20 to 60 °C. PCA was applied to the H NMR spectral data obtained for the LNEs. Read More

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Angela Montanari, Caterina Barone, Michele Barone, and Anna Santangelo: Thermal treatments of canned foods.

Authors:
Jing Chen

Anal Bioanal Chem 2018 Jun 6. Epub 2018 Jun 6.

Merieux NutriSciences (China), 1305 Huajing Road, #10-A, Shanghai, 200231, China.

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Influence of septic system wastewater treatment on titanium dioxide nanoparticle subsurface transport mechanisms.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Department of Chemical and Environmental Engineering, University of California, Riverside, A237 Bourns Hall, Riverside, CA, 92521, USA.

Engineered nanomaterials (ENMs) are commonly incorporated into food and consumer applications to enhance a specific product aspect (i.e., optical properties). Read More

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Two-dimensional capillary electrophoresis-mass spectrometry (CE-CE-MS): coupling MS-interfering capillary electromigration methods with mass spectrometry.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Faculty of Chemistry, Aalen University, Beethovenstrasse 1, 73430, Aalen, Germany.

Electromigration separation techniques often demand certain compounds in the electrolyte to achieve the required selectivity and efficiency. These compounds, including the electrolyte itself, ampholytes, polymeric compounds for sieving, complexing agents, tensides, etc. are often non-volatile. Read More

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Identification and accurate quantification of structurally related peptide impurities in synthetic human C-peptide by liquid chromatography-high resolution mass spectrometry.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Division of Chemical Metrology & Analytical Science, National Institute of Metrology, Beijing, 100029, People's Republic of China.

Peptides are an increasingly important group of biomarkers and pharmaceuticals. The accurate purity characterization of peptide calibrators is critical for the development of reference measurement systems for laboratory medicine and quality control of pharmaceuticals. The peptides used for these purposes are increasingly produced through peptide synthesis. Read More

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June 2018
3 Reads

Gold-coated polydimethylsiloxane microwells for high-throughput electrochemiluminescence analysis of intracellular glucose at single cells.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Department of Respiratory Medicine, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China.

In this communication, a gold-coated polydimethylsiloxane (PDMS) chip with cell-sized microwells was prepared through a stamping and spraying process that was applied directly for high-throughput electrochemiluminescence (ECL) analysis of intracellular glucose at single cells. As compared with the previous multiple-step fabrication of photoresist-based microwells on the electrode, the preparation process is simple and offers fresh electrode surface for higher luminescence intensity. More luminescence intensity was recorded from cell-retained microwells than that at the planar region among the microwells that was correlated with the content of intracellular glucose. Read More

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June 2018
1 Read

Correction to: Branched-chain dicationic ionic liquids for fatty acid methyl ester assessment by gas chromatography.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX, 76019, USA.

The authors would like to call the reader's attention to the fact that the original publication included some corrections needed to be addressed. Read More

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June 2018
1 Read

Development of a method for the simultaneous determination of multi-class pesticides in earthworms by liquid chromatography coupled to tandem electrospray mass spectrometry.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Ens de Lyon, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, 69100, Villeurbanne, France.

Agricultural intensification, and in particular the use of pesticides, leads over the years to a loss of biodiversity and a decline of ecosystem services in cultivated zones and agricultural landscapes. Among the animal communities involved in the functioning of agro-ecosystems, earthworms are ubiquitous and recognized as indicators of land uses and cultural practices. However, little data is available on the levels of pesticides in such organisms in natura, which would allow estimating their actual exposure and the potentially resulting impacts. Read More

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Smart app-based on-field colorimetric quantification of mercury via analyte-induced enhancement of the photocatalytic activity of TiO-Au nanospheres.

Anal Bioanal Chem 2018 Jun 4. Epub 2018 Jun 4.

Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan.

We have devised a unique strategy for highly sensitive, selective, and colorimetric detection of mercury based on analyte-induced enhancement of the photocatalytic activity of TiO-Au nanospheres (TiO-Au NSs) toward degradation of methylene blue (MB). Through electrostatic interactions, Au nanoparticles are attached to poly-(sodium 4-styreneulfonate)/poly(diallyldimethylammonium chloride) modified TiO nanoparticles, which then form an Au shell on each TiO core through reduction of Au with ascorbic acid. Notably, the deposition of Hg species (Hg/CHHg) onto TiO-Au NSs through strong Au-Hg aurophilic interactions speeds up catalytic degradation of MB. Read More

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Insight into the biological effects of acupuncture points by X-ray absorption fine structure.

Anal Bioanal Chem 2018 Jun 2. Epub 2018 Jun 2.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230029, Anhui, China.

Exploration of the biological effects of transition metal ions in acupuncture points is essential to clarify the functional mechanism of acupuncture treatment. Here we show that in the SP6 acupuncture point (Sanyinjiao) the Fe ions are in a high-spin state of approximately te in an Fe-N(O) octahedral crystal field. The Fe K-edge synchrotron radiation X-ray absorption fine structure results reveal that the Fe-N and Fe-O bond lengths in the SP6 acupuncture point are 2. Read More

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Rui Yang: Analytical methods for polymer characterization.

Authors:
Albena Lederer

Anal Bioanal Chem 2018 May 31. Epub 2018 May 31.

Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Str. 6, 01069, Dresden, Germany.

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Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry.

Anal Bioanal Chem 2018 May 30. Epub 2018 May 30.

Department of Chemistry, University of California Riverside, 900 University Ave., Riverside, CA, 92521, USA.

Nanoparticles (NPs) tend to adsorb matrix molecules like proteins and lipids incubated with biological fluids, forming a biological corona. While the formation and functions of protein corona have been studied extensively, little attention has been paid to lipid adsorption on NPs. However, lipids are also abundantly present in biological fluids and play important roles in processes like cell signaling and angiogenesis. Read More

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May 2018
5 Reads

Separation of hydrophobically modified hyaluronic acid according to the degree of substitution by gradient elution high performance liquid chromatography.

Anal Bioanal Chem 2018 May 30. Epub 2018 May 30.

Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag, X1, Matieland, 7602, South Africa.

Amphiphilic hyaluronic acid (HA), synthesised by modifying HA to varying extents with acrylate groups, was successfully separated according to degree of substitution (DS) using solvent gradient high performance liquid chromatography (HPLC). Two HPLC methods based on the amphiphilic structure of the HA were developed. In the first approach, normal phase gradient HPLC was explored, and separation was based on the interactions of HA's polar hydroxyl groups with a polar cyano stationary phase. Read More

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European analytical column number 46.

Anal Bioanal Chem 2018 May 30. Epub 2018 May 30.

Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11222, Belgrade, Serbia.

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May 2018
1 Read

Chiral capillary electrophoresis with UV-excited fluorescence detection for the enantioselective analysis of 9-fluorenylmethoxycarbonyl-derivatized amino acids.

Anal Bioanal Chem 2018 May 29. Epub 2018 May 29.

Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

The potential of capillary electrophoresis (CE) with ultraviolet (UV)-excited fluorescence detection for sensitive chiral analysis of amino acids (AAs) was investigated. DL-AAs were derivatized with 9-fluorenylmethoxycarbonyl chloride (FMOC)-Cl to allow their fluorescence detection and enhance enantioseparation. Fluorescence detection was achieved employing optical fibers, leading UV excitation light (< 300 nm) from a Xe-Hg lamp to the capillary window, and fluorescence emission to a spectrograph equipped with a charge-coupled device (CCD). Read More

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Proteins and antibodies in serum, plasma, and whole blood-size characterization using asymmetrical flow field-flow fractionation (AF4).

Anal Bioanal Chem 2018 May 29. Epub 2018 May 29.

Department of Food Technology, Engineering and Nutrition, Faculty of Engineering LTH, Lund University, 22100, Lund, Sweden.

The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i. Read More

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Development and validation of a comprehensive solid-phase extraction method followed by LC-TOF/MS for the analysis of eighteen pharmaceuticals in influent and effluent of sewage treatment plants.

Anal Bioanal Chem 2018 May 28. Epub 2018 May 28.

Malaysia-Japan International Institute of Technology (MJIIT), Universiti Teknologi Malaysia (UTM), Jalan Sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia.

The scarcity of data about the occurrence of pharmaceuticals in water bodies in Malaysia prompted us to develop a suitable analytical method to address this issue. We therefore developed a method based on solid-phase extraction combined with liquid chromatography-time of flight/mass spectrometry (SPE-LC-TOF/MS) for the analysis of sixteen prescribed and two nonprescribed pharmaceuticals that are potentially present in water samples. The levels of these pharmaceuticals, which were among the top 50 pharmaceuticals consumed in Malaysia during the period 2011-2014, in influent and effluent of five sewage treatment plants (STPs) in Bangi, Malaysia, were then analyzed using the developed method. Read More

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Characterization of the aroma profile of novel Brazilian wines by solid-phase microextraction using polymeric ionic liquid sorbent coatings.

Anal Bioanal Chem 2018 May 28. Epub 2018 May 28.

Institute of Chemistry, Universidade Estadual de Campinas, 270 Monteiro Lobato, Campinas, SP, 13083-970, Brazil.

In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from 'Isabella' and 'BRS Magna' cultivars-the latter was recently introduced by the Brazilian Agricultural Research Corporation - National Grape & Wine Research Center. The structurally tuned SPME coatings were compared to the commercial SPME phases, namely poly(acrylate) (PA) and divinylbenzene/carboxen/poly(dimethylsiloxane) (DVB/CAR/PDMS). The separation, detection and identification of the aroma profiles were obtained using comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). Read More

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Hydrophilic interaction liquid chromatography in the separation of glycopeptides and their isomers.

Anal Bioanal Chem 2018 May 28. Epub 2018 May 28.

Department of Oncology, Lombardi Comprehensive Cancer Center PSB GF9, Georgetown University, 3800 Reservoir Road NW, Washington, DC, 20057, USA.

The analysis of intact glycopeptides is a challenge because of the structural variety of the complex conjugates. In this work, we used separation involving hydrophilic interaction liquid chromatography using a superficially porous particle HALO® penta-HILIC column with tandem mass spectrometric detection for the analysis of N-glycopeptides of hemopexin. We tested the effect of the mobile phase composition on retention and separation of the glycopeptides. Read More

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Distributed electrochemical sensors: recent advances and barriers to market adoption.

Anal Bioanal Chem 2018 Jul 28;410(17):4077-4089. Epub 2018 May 28.

Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Campus Sescelades, c/. Marcel·lí Domingo, 1, 43007, Tarragona, Spain.

Despite predictions of their widespread application in healthcare and environmental monitoring, electrochemical sensors are yet to be distributed at scale, instead remaining largely confined to R&D labs. This contrasts sharply with the situation for physical sensors, which are now ubiquitous and seamlessly embedded in the mature ecosystem provided by electronics and connectivity protocols. Although chemical sensors could be integrated into the same ecosystem, there are fundamental issues with these sensors in the three key areas of analytical performance, usability, and affordability. Read More

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LC/MS analysis of vitamin D metabolites by dielectric barrier discharge ionization and a comparison with electrospray ionization and atmospheric pressure chemical ionization.

Anal Bioanal Chem 2018 May 26. Epub 2018 May 26.

Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 30, 48149, Münster, Germany.

Serum vitamin D metabolite levels are of interest as biomarkers for vitamin D status, which has influence on numerous body functions and pathologies. The determination of vitamin D metabolite levels by liquid chromatography/mass spectrometry (LC/MS) is challenging due to their low concentrations and relatively low ionization efficiencies. Three ionization sources, dielectric barrier discharge ionization (DBDI), atmospheric pressure chemical ionization (APCI), and electrospray ionization (ESI), were compared regarding achievable limits of detection and occurring matrix effects. Read More

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Metrology for stable isotope reference materials: C/C and O/O isotope ratio value assignment of pure carbon dioxide gas samples on the Vienna PeeDee Belemnite-CO scale using dual-inlet mass spectrometry.

Anal Bioanal Chem 2018 Jul 25;410(17):4153-4163. Epub 2018 May 25.

Surface and Trace Chemical Analysis Group, Materials Measurement Science Division, Materials Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.

Isotope ratio measurements have been conducted on a series of isotopically distinct pure CO gas samples using the technique of dual-inlet isotope ratio mass spectrometry (DI-IRMS). The influence of instrumental parameters, data normalization schemes on the metrological traceability and uncertainty of the sample isotope composition have been characterized. Traceability to the Vienna PeeDee Belemnite(VPDB)-CO scale was realized using the pure CO isotope reference materials(IRMs) 8562, 8563, and 8564. Read More

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Negative-ion atmospheric pressure ionisation of semi-volatile fluorinated compounds for ultra-high-performance liquid chromatography tandem mass spectrometry analysis.

Anal Bioanal Chem 2018 May 24. Epub 2018 May 24.

Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain.

In this work, the feasibility of negative-ion atmospheric pressure chemical ionisation (APCI) and atmospheric pressure photoionisation (APPI) for ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) determination of fluorotelomer alcohols (FTOHs), fluorinated octanesulfonamides (FOSAs) and fluorinated octanesulfonamido-ethanols (FOSEs) was evaluated. The study of the effect of mobile phase composition on the atmospheric pressure ionisation of these compounds indicated that methanol/water mixtures provided the best responses in APCI, while acetonitrile/water with a post-column addition of toluene as dopant was the most appropriated mixture in APPI. Under the optimal working conditions, most of the target compounds produced the ion [M-H] as base peak, although in-source collision-induced dissociation fragment ions in APCI and APPI and superoxide adduct ions [M+O] in APPI were also present. Read More

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Rapid detection and differentiation of Staphylococcus colonies using an optical scattering technology.

Anal Bioanal Chem 2018 May 24. Epub 2018 May 24.

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN, 47907-2009, USA.

Staphylococcus species are a major pathogen responsible for nosocomial infections and foodborne illnesses. We applied a laser-based BARDOT (bacterial rapid detection using optical scattering technology) for rapid colony screening and detection of Staphylococcus on an agar plate and differentiate these from non-Staphylococcus spp. Among the six growth media tested, phenol red mannitol agar (PRMA) was found most suitable for building the Staphylococcus species scatter image libraries. Read More

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Monitoring dynamic release of intracellular hydrogen peroxide through a microelectrode based enzymatic biosensor.

Anal Bioanal Chem 2018 May 24. Epub 2018 May 24.

State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

A high sensitive and selective hydrogen peroxide (HO) biosensor was fabricated on the basis of reduced hemoglobin (Hb) and single-walled carbon nanotubes (SWCNTs) for detecting the release of HO from living HepG2 cancer cells in the process of the in situ biosynthesis of ZnO quantum. The modification of carbon fiber microelectrode (CFME) was carried out by physical adsorption. By the scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), the dense cover of surface and successful immobilization were characterized. Read More

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May 2018
1 Read

Trace analysis of multi-class phytohormones in Oryza sativa using different scan modes in high-resolution Orbitrap mass spectrometry: method validation, concentration levels, and screening in multiple accessions.

Anal Bioanal Chem 2018 May 23. Epub 2018 May 23.

Research Group EnVOC, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Ghent, Belgium.

Phytohormones are signaling and regulating metabolites involved in numerous plant processes, including growth, development, and responses to stress. Currently, the focus is on the analysis of multiple phytohormones in order to characterize crosstalk and hormone signaling networks. In this paper, representative phytohormones of the major classes are simultaneously determined in rice tissues by a generic solid-liquid extraction, followed by liquid chromatography and electrospray ionization high-resolution tandem mass spectrometry using a Q-Exactive™ instrument. Read More

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An enzyme-free homogenous electrochemical assay for sensitive detection of the plasmid-mediated colistin resistance gene mcr-1.

Anal Bioanal Chem 2018 May 22. Epub 2018 May 22.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou, 510515, Guangdong, China.

Antibiotic resistance associated with the mcr-1 gene of Gram-negative bacteria, which confers resistance to drugs of last resort and has the potential to spread via plasmids, is one of the most pressing issues facing global health today. Point-of-care testing for the mcr-1 gene is needed to aid in the identification of colistin resistance in the field and to control its horizontal transmission. Here, we report the successful development of an enzyme-free homogenous electrochemical strategy for sensitive detection of the antibiotic resistance gene mcr-1 using the hybridization chain reaction and mcr-1-specific toehold probe. Read More

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May 2018
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