147 results match your criteria Analytical Methods[Journal]


Optical Nanosensors for Physiological Chloride Detection for Monitoring Cystic Fibrosis Treatment.

Anal Methods 2020 Mar 26;12(11):1441-1448. Epub 2020 Feb 26.

Department of Bioengineering, Northeastern University, Boston, Massachusetts 02115, United States.

Personalized approaches for continuous monitoring of chloride levels are potentially valuable for evaluating the efficacy of new treatments of genetic disorders such as cystic fibrosis. In this report, we validated optode-based nanosensors for real-time chloride monitoring in the interstitial fluid of living animals. These nanosensors take advantage of a ratiometric sensing scheme which demonstrates reversible and selective chloride detection in the physiological range. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY02717CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100910PMC

Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs).

Anal Methods 2020 Jan 26;12(1):18-24. Epub 2019 Nov 26.

Department of Chemistry, University of North Carolina at Chapel Hill, Kenan and Caudill Laboratories, 125 South Road, Chapel Hill, NC, 27599-3290, USA.

Oxygen is a transcriptional regulator responsible for tissue homeostasis and maintenance. Studies relating cellular phenotype with oxygen tension often use hypoxia chambers, which expose cells to a single, static oxygen tension. Despite their ease of use, these chambers are unable to replicate the oxygen gradients found in healthy and diseased tissues. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY01690BDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079814PMC
January 2020

MICROSCALE SERUM EXTRACTION METHOD FOR THE SIMULTANEOUS ANALYSIS OF CORTICOSTERONE AND LIPIDS.

Anal Methods 2019 Dec 29;11(45):5746-5749. Epub 2019 Oct 29.

Department of Chemistry, University of Nebraska - Lincoln, Lincoln, NE, 68588-0304, USA.

Corticosterone is an important steroid for the regulation of metabolism and stress response. Existing methods for the measurement of corticosterone include radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA), and liquid chromatography-mass spectrometry (LC-MS). While each of these approaches have their advantages, RIAs use radioactive isotopes that necessitate specially regulated usage and disposal. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9ay01757gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059636PMC
December 2019

Review of 3D Cell Culture with Analysis in Microfluidic Systems.

Anal Methods 2019 Sep 6;11(33):4220-4232. Epub 2019 Aug 6.

Department of Chemistry, Saint Louis University, 3501 Laclede Ave., St. Louis, MO 63103.

A review with 105 references that analyzes the emerging research area of 3D cell culture in microfluidic platforms with integrated detection schemes. Over the last several decades a central focus of cell culture has been the development of better mimics. This has led to the evolution from planar cell culture to cell culture on 3D scaffolds, and the incorporation of cell scaffolds into microfluidic devices. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9ay01328hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015157PMC
September 2019

A disposable acetylcholine esterase sensor for As(III) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis.

Anal Methods 2019 Sep;11(40):5203-5213

National Risk Management Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, Ohio, 45268, USA.

There is a lack of field compatible analytical method for the speciation of As(III) to characterize groundwater pollution at anthropogenic sites. To address this issue, an inhibition-based acetylcholine esterase (AchE) sensor was developed to determine As(III) in groundwater. 4-Acetoxyphenol was employed to develop an amperometric assay for AchE activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY01199DDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997941PMC
September 2019

A microfluidic platform integrating pressure-driven and electroosmotic-driven flow with inline filters for affinity separations.

Anal Methods 2019 Dec 29;11(45):5768-5775. Epub 2019 Oct 29.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Dittmer Building, Tallahassee, FL 32306, USA.

Pancreatic islets of Langerhans release glucagon to maintain blood glucose levels, and release of this peptide is dysregulated in diabetes mellitus. Although the importance of proper secretion of this peptide has been shown, no measurement of its release at the single islet level has been reported. In previous work, a non-competitive assay for glucagon was developed with a 6 pM limit of detection, low enough to measure from a single islet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY01758EDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980329PMC
December 2019

A rapid and adaptable lipidomics method for quantitative UPLC-mass spectrometric analysis of phosphatidylethanolamine and phosphatidylcholine , and in cells.

Anal Methods 2019 Apr 12;11(13):1765-1776. Epub 2019 Mar 12.

Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL 33620.

Phosphatidylethanolamine (PE) and phosphatidylcholine (PC) are highly prevalent phospholipids in mammalian membranes. There are currently no methods for detection of minute levels of these phospholipids or simultaneously with products of the utilization of these phospholipid substrates by phospholipase A (PLA) enzymes. To examine the substrate utilization of PE and PC by PLA, we developed a method to accurately detect and measure specific forms of PE and PC as low as 50 femtomoles. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY00052FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884326PMC

Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application.

Anal Methods 2019 Jan 15;11(1):49-57. Epub 2018 Nov 15.

Advanced Technology Core, Dan L. Duncan Cancer Center, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

Methylation aberrations play an important role in many metabolic disorders including cancer. Methylated metabolites are direct indicators of metabolic aberrations, and currently, there is no Liquid chromatography - Mass spectrometry (LC-MS) based method available to cover all classes of methylated metabolites at low detection limits. In this study, we have developed a method for the detection of methylated metabolites, and it's biological application. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02168FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874373PMC
January 2019

Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance.

Anal Methods 2019 Jan 17;11(3):296-302. Epub 2018 Dec 17.

Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA. ; Tel: +1-574-631-1849.

A siderophore-based active bacterial pull-down strategy was integrated in a localized surface plasmon resonance (LSPR) sensing platform and subsequently tested by detecting whole-cell . The LSPR-based whole-cell sensing approach was previously demonstrated with aptamer-based molecular recognition motifs, and here it is extended to the powerful siderophore system, which exploits the natural bacterial need to sequester Fe(III). Specifically, a biscatecholate-monohydroxamate mixed ligand siderophore linked to a biotin three polyethylene glycol repeating units was synthesized and immobilized on Au trigonal nanoprisms of an LSPR sensor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02180EDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820853PMC
January 2019

A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids.

Anal Methods 2019 Feb 24;11(8):1027-1034. Epub 2019 Jan 24.

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.

Early and accurate diagnosis is crucial to monitor infection outcomes and provide timely interventions. However, gold standard polymerase chain reaction assays (PCR) are labor-intensive and require expensive reagents and instrumentation. Nuclease protection has been used for decades to detect and quantify nucleic acid but has not yet been investigated as a diagnostic tool for infectious disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02729CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814143PMC
February 2019

Capillary zone electrophoresis-tandem mass spectrometry using ultraviolet photodissociation (213 nm) for large-scale top-down proteomics.

Anal Methods 2019 Jun 7;11(22):2855-2861. Epub 2019 May 7.

Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, United States.

Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has attracted attention recently for large-scale top-down proteomics that aims to characterize proteoforms in cells at a global scale and with high throughput. In this work, CZE-MS/MS with ultraviolet photodissociation (UVPD) was evaluated for large-scale top-down proteomics for the first time. Roughly, 600 proteoforms and 369 proteins were identified from a zebrafish brain sample via coupling size exclusion chromatography (SEC) fractionation to CZE-UVPD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C9AY00585DDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788745PMC

Optimization of a microchip electrophoresis method with electrochemical detection for the determination of nitrite in macrophage cells as an indicator of nitric oxide production.

Anal Methods 2019 Jan 26;11(2):148-156. Epub 2018 Nov 26.

Department of Chemistry, University of Kansas, Lawrence, KS, USA.

Nitric oxide (NO) is involved in many biological functions, including blood pressure regulation, the immune response, and neurotransmission. However, excess production of NO can lead to the generation of reactive nitrogen species and nitrosative stress and has been linked to several neurodegenerative diseases and cardiovascular disorders. Because NO is short-lived and generally difficult to detect, its primary stable degradation product, nitrite, is frequently monitored in its place. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02014KDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774641PMC
January 2019

Ultrasensitive Multi-Species Detection of CRISPR-Cas9 by a Portable Centrifugal Microfluidic Platform.

Anal Methods 2019 Feb 3;11(5):559-565. Epub 2019 Jan 3.

Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94550, USA.

The discovery of the RNA-guided DNA nuclease CRISPR-Cas9 has enabled the targeted editing of genomes from diverse organisms, but the permanent and inheritable nature of genome modification also poses immense risks. The potential for accidental exposure, malicious use, or undesirable persistence of Cas9 therapeutics and off-target genome effects highlight the need for detection assays. Here we report a centrifugal microfluidic platform for the measurement of both Cas9 protein levels and nuclease activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02726ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764754PMC
February 2019
1 Read

An improved rapid sampling microdialysis system for human and porcine organ monitoring in a hospital setting.

Anal Methods 2018 Nov 5;10(44):5273-5281. Epub 2018 Nov 5.

Department of Bioengineering , Imperial College London , UK . Email:

Online organ monitoring could provide clinicians with critical information regarding organ health prior to transplantation and could aid clinical decision-making. This paper presents the methodology of online microdialysis for real-time monitoring of human organs . We describe how rapid sampling microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine pancreases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8ay01807cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244488PMC
November 2018

Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow.

Anal Methods 2019 Jan 3;11(1):8-16. Epub 2018 Dec 3.

Department of Electrical and Computer Engineering , University of Houston , USA . Email:

, microvasculature provides oxygen, nutrients, and soluble factors necessary for cell survival and function. The highly tortuous, densely-packed, and interconnected three-dimensional (3D) architecture of microvasculature ensures that cells receive these crucial components. The ability to duplicate microvascular architecture in tissue-engineered models could provide a means to generate large-volume constructs as well as advanced microphysiological systems. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8ay01798kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336169PMC
January 2019

Integrating 3D Cell Culture of PC12 Cells with Microchip-Based Electrochemical Detection.

Anal Methods 2019 Feb 29;11(8):1064-1072. Epub 2019 Jan 29.

Department of Chemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, MO 63103.

Developing cell culture models that accurately mimic processes in a manner that also enables near real-time analysis of neurotransmitters is an important research area. New technologies being developed such as 3D scaffolds for cell culture and 3D printed microfluidics provide an opportunity for such advancements. In this work, PC12 cells were used as a model system and they were immobilized onto a 3D scaffold of polystyrene (PS) fibers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02672FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594695PMC
February 2019

Simple lateral flow assays for microbial detection in stool.

Anal Methods 2018 Dec 20;10(45):5358-5363. Epub 2018 Sep 20.

GoDx , 510 Charmany Drive, Suite 257 , Madison , WI 53719 , USA . Email:

Diarrheal diseases claim the lives of 1300 children daily, mostly in the developing world. We have developed a simple lateral flow assay capable of detecting and EPEC DNA and RNA rapidly (<15 minutes) at the point-of-need, directly from stool without nucleic acid extraction or molecular amplification. The limit of detection of the method is 1 nM using synthetic DNA target substrates spiked into stool. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8ay01475bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6253687PMC
December 2018
3 Reads

Multi-waveform fast-scan cyclic voltammetry mapping of adsorption/desorption kinetics of biogenic amines and their metabolites.

Anal Methods 2018 Jun 23;10(24):2834-2843. Epub 2018 May 23.

Department of Biomedical Engineering, Hanyang University, Seoul, Korea.

Fast-scan cyclic voltammetry (FSCV) is an effective method for investigating electro-active neurochemical species. In recent years, FSCV has been used to measure electro-active neurotransmitters in a variety of neuroscience studies. We previously reported on the use of paired-pulse voltammetry (PPV) that enables FSCV to differentiate various analytes and minimize confounding factors by taking advantage of the adsorption characteristics of the analyte on carbon fiber microelectrodes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00352ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532990PMC
June 2018
1 Read

Chiral micellar electrokinetic chromatographic separation for determination of L- and D-primary amines released from murine islets of Langerhans.

Anal Methods 2019 Mar 18;11(9):1276-1283. Epub 2019 Feb 18.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306.

D-amino acids have been located in various tissues including the endocrine portion of the pancreas, the islets of Langerhans. D-Serine (D-Ser), is of particular interest since it is an agonist for the ionotropic N-methyl-D-aspartate receptors. To examine the potential release of D-Ser and other D-amino acids from islets, a chiral micellar electrokinetic chromatography method was developed by derivatizing primary amines with 2,3-naphthalenedicarboxaldehyde and to achieve resolution of the enantiomers, two surfactants were used in the separation, sodium dodecyl sulfate and sodium deoxycholate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8ay02471eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502259PMC
March 2019
10 Reads

Automated chemical identification and library building using dispersion plots for differential mobility spectrometry.

Anal Methods 2018 Sep 14;10(35):4339-4349. Epub 2018 Aug 14.

Mechanical and Aerospace Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Differential mobility spectrometry (DMS) based detectors require rapid data analysis capabilities, embedded into the devices to achieve the optimum detection capabiites as portable trace chemical detectors. Automated algorithm-based DMS dispersion plot data analysis method was applied for the first time to pre-process and separate 3-dimentional (3-D) DMS dispersion data. We previously demonstrated our AnalyzeIMS (AIMS) software was capable of analyzing complex gas chromatography differential mobility spectrometry (GC-DMS) data sets. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00846ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457679PMC
September 2018
3 Reads

Use of 3D Printing and Modular Microfluidics to Integrate Cell Culture, Injections and Electrochemical Analysis.

Anal Methods 2018 Jul 26;10(27):3364-3374. Epub 2018 Jun 26.

Department of Chemistry, Saint Louis University.

Fabrication of microchip-based devices using 3-D printing technology offers a unique platform to create separate modules that can be put together when desired for analysis. A 3-D printed module approach offers various advantages such as file sharing and the ability to easily replace, customize, and modify the individual modules. Here, we describe the use of a modular approach to electrochemically detect the ATP-mediated release of nitric oxide (NO) from endothelial cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00829ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433419PMC
July 2018
1 Read

Automated 4-Sample Protein Immunoassays using 3D-Printed Microfluidics.

Anal Methods 2018 Aug 30;10(32):4000-4006. Epub 2018 Jul 30.

Department of Chemistry and Institute of Material Science, University of Connecticut, Storrs, Connecticut 06269, United States.

Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated, 3D printed electrochemiluminescent (ECL) immunoarray integrated with a nanostructured pyrolytic graphite sheet (PGS) microwell chip configured to detect 2 proteins simultaneously from complex liquid samples with high sensitivity and selectivity. Assays are done in 18 min at cost of < $1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY01271GDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426314PMC
August 2018
1 Read

Droplet Microfluidics in Thermoplastics: Device Fabrication, Droplet Generation, and Content Manipulation using Integrated Electric and Magnetic Fields.

Anal Methods 2018 Sep 20;10(35):4264-4274. Epub 2018 Aug 20.

Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.

We have developed droplet microfluidic devices in thermoplastics and demonstrated the integration of key functional components that not only facilitate droplet generation, but also include electric field-assisted reagent injection, droplet splitting, and magnetic field-assisted bead extraction. We manufactured devices in poly(methyl methacrylate) and cyclic olefin polymer using a hot-embossing procedure employing silicon masters fabricated via photolithography and deep reactive ion etching techniques. Device characterization showed robust fabrication with uniform feature transfer and good embossing yield. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY01474DDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419776PMC
September 2018

Review: New insights into optimizing chemical and 3D surface structures of carbon electrodes for neurotransmitter detection.

Anal Methods 2019 Jan 21;11(3):247-261. Epub 2018 Dec 21.

Dept. of Chemistry, University of Virginia, Charlottesville, VA 22901.

The carbon-fiber microelectrode has been used for decades as a neurotransmitter sensor. Recently, new strategies have been developed for making carbon electrodes, including using carbon nanomaterials or pyrolyzing photoresist etched by nanolithography or 3D printing. This review summarizes how chemical and 3D surface structures of new carbon electrodes are optimized for neurotransmitter detection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02472CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366673PMC
January 2019
1 Read

Direct aqueous injection of the fluoroacetate anion in potable water for analysis by liquid chromatography tandem mass-spectrometry.

Anal Methods 2018 Dec;10(46):5455-5590

U.S. Environmental Protection Agency, Office of Research and Development, National Homeland, Security Research Center, Threat Consequence Assessment Division, 26 Martin Luther King Dr. W., Cincinnati, OH 45268.

Sodium fluoroacetate or Compound 1080 is a rodenticide registered in the United States for use in livestock protection collars. The collars are employed to control predation on herd animals (i.e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY02046ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309164PMC
December 2018

Advancement of analytical modes in a multichannel, microfluidic droplet-based sample chopper employing phase-locked detection.

Anal Methods 2018 Jul 5;10(28):3436-3443. Epub 2018 Jun 5.

Department of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849, USA.

In this work, we expand upon our recently developed droplet-based sample chopping concepts by introducing a multiplexed fluidic micro-chopper device (μChopper). Six aqueous input channels were integrated with a single oil input, and each of these seven channels was controlled by a pneumatic valve for automated sampling through software control. This improved design, while maintaining high precision in valve-based droplet generation at bandwidths of 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00947CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258173PMC
July 2018
3 Reads

Isothermal amplification of long DNA segments by quadruplex priming amplification.

Anal Methods 2018 Jul 29;10(25):2972-2979. Epub 2018 May 29.

Department of Chemistry and Biochemistry, The Ohio State University, Columbus OH 43210, USA.

Amplification of long DNA segments with the highest possible specificity and lowest bias is one of the main goals of modern genomics. Quadruplex priming amplification (QPA) is a single-primer isothermal method, which employs the free energy of quadruplex structures as the driving force for DNA amplification without any extra reaction components. As a result, QPA represents one of the simplest isothermal assays and was previously shown to be suitable for amplification of relatively short DNA sequences. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00843DDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261523PMC
July 2018
2 Reads

A binary matrix for the rapid detection and characterization of small-molecule cardiovascular drugs by MALDI-MS and MS/MS.

Anal Methods 2018 1;10:572-578. Epub 2018 Feb 1.

Division of Pharmaceutical Analysis, Center for Drug Evaluation and Research, Food and Drug Administration, St. Louis, MO, 63110, USA.

A mixture of α-cyano-4-hydroxycinnamic acid and 1,5-diaminonaphthalene was discovered as a novel binary matrix for the qualitative analysis of 14 small-molecule (~250-550 Da) cardiovascular drugs by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and MS/MS in either positive or negative ion mode. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C7AY02583A
Publisher Site
http://dx.doi.org/10.1039/C7AY02583ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178826PMC
February 2018
1 Read

Multiplexed microRNA Expression Profiling by Combined Asymmetric PCR and Label-Free Detection using Silicon Photonic Sensor Arrays.

Anal Methods 2018 Apr 23;10(14):1618-1623. Epub 2018 Mar 23.

Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Matthews Ave., Urbana, IL 61801, USA.

Analysis methods based upon the quantitative, real-time polymerase chain reaction are extremely powerful; however, they face intrinsic limitations in terms of target multiplexing. In contrast, silicon photonic microring resonators represent a modularly multiplexable sensor array technology that is well-suited to the analysis of targeted biomarker panels. In this manuscript we employ an asymmetric polymerase chain reaction approach to selectively amplify copies of cDNAs generated from targeted miRNAs before multiplexed, label-free quantitation through hybridization to microring resonator arrays pre-functionalized with capture sequences. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00190ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162071PMC
April 2018
3 Reads

Continuous monitoring of adenosine and its metabolites using microdialysis coupled to microchip electrophoresis with amperometric detection.

Anal Methods 2018 Aug 13;10(30):3737-3744. Epub 2018 Jul 13.

Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA.

Rapid monitoring of concentration changes of neurotransmitters and energy metabolites is important for understanding the biochemistry of neurological disease as well as for developing therapeutic options. This paper describes the development of a separation-based sensor using microchip electrophoresis (ME) with electrochemical (EC) detection coupled to microdialysis (MD) sampling for continuous on-line monitoring of adenosine and its downstream metabolites. The device was fabricated completely in PDMS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY01041BDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774626PMC

A New Wipe-Sampling Instrument for Measuring the Collection Efficiency of Trace Explosives Residues.

Anal Methods 2018 Jan 4;10(2):204-213. Epub 2017 Dec 4.

National Institute of Standards and Technology, Materials Measurement Science Division, Gaithersburg, MD, USA.

Trace explosives detection, a crucial component of many security screening environments, commonly employs wipe-sampling. Since collection of an explosive residue is necessary for detection, it is important to have a thorough understanding of the parameters that affect the efficiency of collection. Current wipe-sampling evaluation techniques for explosive particles have their limits: manual sampling (with fingers or a wand) is limited in its ability to isolate a single parameter and the TL-slip/peel tester is limited to a linear sample path. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY02694CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986101PMC
January 2018
2 Reads

Dual polarity MALDI imaging mass spectrometry on the same pixel points reveals spatial lipid localizations at high-spatial resolutions in rat small intestine.

Anal Methods 2018 Jun 3;10(21):2428-2435. Epub 2018 May 3.

The Gothenburg Imaging Mass Spectrometry (Go:IMS) Platform , University of Gothenburg and Chalmers University of Technology , Gothenburg 412 96 , Sweden . Email:

Sensitive laser desorption/ionization obtained a sublimation-coated 1,5-diaminonaphthalene (1,5-DAN) matrix allowed dual polarity MALDI-IMS analysis on the same pixel points across the jejunal mucosal region in rat small intestine which yielded high-spatial-resolution (10 μm) ion images of several lipid species correlated with the same histological features. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8ay00645hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985652PMC

Enhanced Microchip Electrophoresis Separations Combined with Electrochemical Detection Utilizing a Capillary Embedded in Polystyrene.

Anal Methods 2018 Jan 6;10(1):37-45. Epub 2017 Dec 6.

Department of Chemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, MO 63103.

The ability to use microchip-based electrophoresis for fast, high-throughput separations provides researchers with a tool for close-to real time analysis of biological systems. While PDMS-based electrophoresis devices are popular, the separation efficiency is often an issue due to the hydrophobic nature of PDMS. In this study, a hybrid microfluidic capillary device was fabricated to utilize the positive features of PDMS along with the electrophoretic performance of fused silica. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY02505JDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915312PMC
January 2018
8 Reads

Dissecting the Dissociation Patterns of Fucosylated Glycopeptides Undergoing CID: A Case Study in Improving Automated Glycopeptide Analysis Scoring Algorithms.

Anal Methods 2018 8;10(2):256-262. Epub 2017 Dec 8.

Ralph N Adams Institute for Bioanalytical Chemistry, Department of Chemistry, University of Kansas, Lawrence, Kansas-66047, United States.

The need to investigate the fragmentation of fucosylated glycopeptides is driven by recent work showing that at least one, and perhaps many, glycopeptide analysis scoring algorithms are less effective at identifying fucosylated glycopeptides than non-fucosylated glycopeptides. Herein, we study the CID fragmentation characteristics of fucosylated glycopeptides and the scoring rules of the glycopeptide analysis software, GlycoPep Grader, in an effort to improve automated assignments of these important glycopeptides. We identified some prominent product ions from a common fragmentation pathway of fucosylated glycopeptides that were not accounted for in the scoring rules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY02687KDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898446PMC
December 2017
5 Reads

Paper Spray Mass Spectrometry for High-Throughput Quantification of Nicotine and Cotinine.

Anal Methods 2018 28;10(1):46-50. Epub 2017 Nov 28.

Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA.

The rapid release of new tobacco products requires high-throughput quantitative methods to support tobacco research. Sample preparation for LC-MS and GC-MS is time consuming and limits throughput. Paper spray tandem mass spectrometry (PS-MS/MS) is proposed and validated as a simple and rapid method for quantification of nicotine and cotinine in complex matrices to support tobacco-related research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY02204BDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858713PMC
November 2017
34 Reads

SERS-fluorescence bimodal nanoprobes for in vitro imaging of fatty acid responsive receptor GPR120.

Anal Methods 2018 18;10(1):22-29. Epub 2017 Oct 18.

Department of Biological Engineering, Utah State University, Logan, Utah 84322-4105, U.S.A.

G-protein-coupled receptor 120 (GPR120), as a member of the rhodopsin family of G-protein-coupled receptors, has been shown to function as a sensor for dietary fat in the gustatory and digestive systems. Its specific role in the chemoreception of fatty acids, which is thought to be crucial in understanding the mechanism surrounding the control of fat intake and, accordingly, in the treatment of obesity, remains unclear. Here we report a novel surface-enhanced Raman spectroscopy (SERS)-fluorescence bimodal microscopic technique for detection and imaging of GPR120 in single living cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY02039BDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808993PMC
October 2017
2 Reads

Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in Standard Reference Material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection.

Anal Methods 2018 ;10

Department of Chemistry, University of Central Florida, Orlando, Florida 32816.

The identification of isomeric polycyclic aromatic hydrocarbons (PAHs) in complex samples via reversed-phase liquid chromatography (RPLC) with fluorescence detection (FL) is normally based on matching the chromatographic retention times of suspected peaks of interest with reference standards. Since no spectral information is obtained during the chromatographic run, the accurate identification of co-eluting PAHs with similar chromatographic behaviors requires confirmation with additional chromatographic methods. This is particularly true for the analysis of PAH isomers with the relative molecular mass (MM, g/mol) 302. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C8AY00760HDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512832PMC
January 2018

Fast Quantitation of Pyrazole Fungicides in Wine by Ambient Ionization Mass Spectrometry.

Anal Methods 2017 26;9(34):5058-5064. Epub 2017 Jun 26.

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China.

Fungicides are widely used for growing the grapes that are used for making wines. Chromatography coupled with tandem mass spectrometry is usually time and labor consuming for quantitation of fungicides in wines. In this work, a simple ambient mass spectrometry method using paper capillary spray was developed for the fast quantitation of four pyrazole fungicides in wines. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY01534HDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731662PMC
June 2017
6 Reads

Biomonitoring method for the analysis of chromium and cobalt in human whole blood using inductively coupled plasma - kinetic energy discrimination - mass spectrometry (ICP-KED-MS).

Anal Methods 2017 19;9(23):3464-3476. Epub 2017 Apr 19.

Inorganic & Radiation Analytical Toxicology Branch, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F-50, Atlanta, GA 30341-3717.

The Centers for Disease Control and Prevention developed a biomonitoring method to rapidly and accurately quantify chromium and cobalt in human whole blood by ICP-MS. Many metal-on-metal hip implants which contain significant amounts of chromium and cobalt are susceptible to metal degradation. This method is used to gather population data about chromium and cobalt exposure of the U. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C7AY00430C
Publisher Site
http://dx.doi.org/10.1039/C7AY00430CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709816PMC
April 2017
22 Reads

A novel method of nicotine quantification in electronic cigarette liquids and aerosols.

Anal Methods 2017 Aug 19;9(29):4261-4266. Epub 2017 Jun 19.

Department of Chemical Engineering, University of Louisville, Louisville, KY 40208.

The electronic cigarette (e-cigarette) has emerged as popular electronic nicotine delivery devices (ENDs). However, the general safety and validity of e-cigarettes for nicotine delivery efficacy are still not well understood. This study developed a new method for efficient measurement of nicotine levels in both the liquids (e-liquids) used in e-cigarettes and the aerosols generated from the e-cigarettes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY00501FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703602PMC
August 2017
5 Reads
1.821 Impact Factor

Quantitation of Fentanyl Analogs in Dried Blood Spots by Flow-Through Desorption Coupled to Online Solid Phase Extraction Tandem Mass Spectrometry.

Anal Methods 2017 16;9:3876-3883. Epub 2017 Jun 16.

Centers for Disease Control and Prevention, 4770 Buford Hwy, MS F44, Atlanta, GA 30341.

An automated dried blood spot (DBS) elution coupled with solid phase extraction and tandem mass spectrometric analysis for multiple fentanyl analogs was developed and assessed. This method confirms human exposures to fentanyl, sufentanil, carfentanil, alfentanil, lofentanil, α-methyl fentanyl, and 3-methyl fentanyl in blood with minimal sample volume and reduced shipping and storage costs. Seven fentanyl analogs were detected and quantitated from DBS made from venous blood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY00532FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701286PMC
June 2017
22 Reads

Surface acoustic wave devices for chemical sensing and microfluidics: A review and perspective.

Anal Methods 2017 13;9(28):4112-4134. Epub 2017 Jun 13.

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA.

Surface acoustic waves (SAWs), are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for chemical sensing and analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY00690JDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685524PMC
June 2017
2 Reads

Imaging Acetaldehyde Formation During Ethanol Metabolism in Living Cells using a Hydrazinyl Naphthalimide Fluorescent Probe.

Anal Methods 2017 29;9(23):3418-3421. Epub 2017 May 29.

Department of Chemistry, Southern Methodist University, Dallas, TX 75275-0314, USA.

The metabolism of ethanol to acetaldehyde has been visualized in living lung epithelial cells using a hydrazinyl naphthalimide fluorescent probe. Utilizing a condensation reaction between carbonyls and a hydrazine moeity, we demonstrate that the fluorescent probe (Aldehydefluor-1) AF1 reacts with a range of reactive carbonyl species including formaldehyde, acetaldehyde, glyoxylic acid, and methyl glyoxal. With AF1, it is possible to directly visualize endogenous carbonyl metabolites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY01238ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669384PMC

Monitoring carnosine uptake by RAW 264.7 macrophage cells using microchip electrophoresis with fluorescence detection.

Anal Methods 2017 Jan 14;9(3):402-408. Epub 2016 Dec 14.

Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, Kansas.

Carnosine, a dipeptide found in a variety of tissues, is believed to possess antioxidant properties. It serves as a scavenger of reactive nitrogen and oxygen species (RNOS), which are important stress mediators of pro-inflammatory conditions and can lead to macrophage activation. In this study, intracellular concentrations of carnosine in murine RAW 264. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C6AY03009BDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663230PMC
January 2017
2 Reads

Metabolic Comparison of Dorsal Ventral Cells Directly in the Live 8-cell Frog Embryo by Microprobe Single-cell CE-ESI-MS.

Anal Methods 2017 Sep 9;9(34):4964-4970. Epub 2017 May 9.

Department of Chemistry, The George Washington University, Washington DC, 20052.

Single-cell mass spectrometry (MS) empowers the characterization of metabolomic changes as cells differentiate to different tissues during early embryogenesis. Using whole-cell dissection and capillary electrophoresis electrospray ionization (CE-ESI) MS, we recently uncovered metabolic cell-to-cell differences in the 8- and 16-cell embryo of the South African clawed frog (), raising the question whether metabolic cell heterogeneity is also detectable across the dorsal-ventral axis of the 8-cell embryo. Here, we tested this hypothesis directly in the live embryo by quantifying single-cell metabolism between the left dorsal-animal (D1L) and left ventral-animal (V1L) cell pairs in the same embryo using microprobe single-cell CE-ESI-MS in the positive ion mode. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C7AY00834A
Publisher Site
http://dx.doi.org/10.1039/C7AY00834ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650250PMC
September 2017
2 Reads

Designing a Biostable L-DNAzyme for Lead(II) Ion Detection in Practical Samples.

Anal Methods 2016 Oct 6;8(39):7260-7264. Epub 2016 Sep 6.

Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.

A promising biosensor for effectively lead (II) ion detection in practical applications was developed by constructing a Pb-specific L-DNAzyme, the enantiomer of the natural nucleic acid-constructed D-DNAzyme. This fluorescent sensor contains the L-enzyme strand with a quencher at the 3' end, and the L-substrate strand with a fluorophore at the 5' and a quencher at the 3' ends that formed a complex. In the presence of Pb, the L-substrate is cut into two fragments, leading to the recovery of fluorescence. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C6AY01791FDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650247PMC
October 2016
4 Reads

Microfluidic Iterative Mechanical Characteristics (iMECH) Analyzer for Single-Cell Metastatic Identification.

Anal Methods 2017 Feb 4;9(5):847-855. Epub 2017 Jan 4.

The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, VA 24061, US.

This study describes the development of a microfluidic biosensor called the iterative mechanical characteristics (iMECH) analyzer which enables label-free biomechanical profiling of individual cells for distinction between metastatic and non-metastatic human mammary cell lines. Previous results have demonstrated that pulsed mechanical nanoindentation can modulate the biomechanics of cells resulting in distinctly different biomechanical responses in metastatic and non-metastatic cell lines. The iMECH analyzer aims to move this concept into a microfluidic, clinically more relevant platform. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C6AY03342CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637398PMC
February 2017

SI traceable determination of arsenic species in kelp ().

Anal Methods 2017 Aug 26;9(29):4267-4274. Epub 2017 Jun 26.

National Institute of Standards and Technology, Gaithersburg, MD 20899.

A dietary supplement, kelp contains a significant amount of arsenic that is mostly arsenosugars. The determination of arsenosugars is difficult due to a lack of arsenosugar calibration standard, because arsenosugar compounds are not commercially available. This work reports the determination of arsenicals in a kelp extract with traceability to the International System of Units (SI). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY01111CDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615412PMC
August 2017
10 Reads

Simple method for the quantitative analysis of thin copolymer films on substrates by infrared spectroscopy using direct calibration.

Anal Methods 2017 Sep 21;9(36):5266-5273. Epub 2017 Aug 21.

Institute of Solid State Physics , NAWI Graz , Graz University of Technology , 8010 Graz , Austria . Email:

The chemical composition of a copolymer drives many important material properties and quantification in terms of comonomer volume fraction is thus of practical relevance for many studies. Infrared spectroscopy is one of the most common techniques for compositional analysis but it usually relies on manual evaluation of baselines and peak heights, which can be rather inaccurate and become a laborious task when having multiple spectra to evaluate. On the contrary, Maxwell's theory of electrodynamics can be used to calculate the complex index of refraction from measured spectra promising a more accurate quantification. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7ay01748kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6688560PMC
September 2017

Recent Liquid Chromatographic Approaches and Developments for the Separation and Purification of Carbohydrates.

Anal Methods 2017 Jun 24;9(24):3579-3593. Epub 2017 May 24.

Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.

Carbohydate purification remains a bottleneck in securing analytical standards from natural sources or by chemical or enzymatic synthesis. This review highlights the scope and remaining limitations of recent approaches and methods development in liquid chromatography for robust and higher-throughput carbohydrate separation and isolation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/C7AY01094JDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558844PMC
June 2017
7 Reads