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Anal Methods 2022 May 18. Epub 2022 May 18.

School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan, 114005, China.

Hydrogen peroxide (HO) is a main member of reactive oxygen species (ROS) in cells that has a significant impact on various physiological and pathological processes of organisms. Here, we designed and synthesized a new type of fluorescent probe Rhodol-OAc for the specific detection of HO. The probe had good water solubility, high selectivity and sensitivity to HO, low cytotoxicity, excellent mitochondrial targeting ability, It was successfully applied in the imaging of exogenous and endogenous HO in living cells. Read More

Anal Methods 2022 May 18. Epub 2022 May 18.

Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China.

An ultrasensitive fluorometric oligonucleotide immunoassay (UFOIA) based on a fluorometric oligonucleotide and magnetic separation was proposed for the simultaneous detection of two mycotoxins. Two kinds of magnetic nanoparticle (MNP) probes and their corresponding fluorometric oligonucleotide probes were prepared. After the immune reaction, Cy5-linked and 6-FAM-linked oligonucleotides were dissociated and applied to detect fluorescence signals simultaneously. Read More

Anal Methods 2022 May 14. Epub 2022 May 14.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL 32306, USA.

Islets of Langerhans are the endocrine tissue within the pancreas that secrete hormones for maintenance of blood glucose homeostasis. A variety of small molecules including classical neurotransmitters are also released from islets. While the roles of most of these small molecules are unknown, some have been hypothesized to play a critical role in islet physiology. Read More

Anal Methods 2022 May 13. Epub 2022 May 13.

DCU Water Institute, School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

Poly- and perfluoroalkyl substances (PFASs) are a group of synthetic organic surfactants that have become a global concern because of their toxicity and widespread presence in the aquatic environment and organisms globally. In this study, a new analytical method has been developed and validated for the analysis of 15 perfluorinated compounds in different water matrices: river water, drinking water and seawater. Water extraction was performed in anion exchange solid phase extraction cartridges, and extracts were analysed by liquid chromatography in tandem with mass spectrometry. Read More

Anal Methods 2022 May 13. Epub 2022 May 13.

Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland.

The aim of this study was to develop an effective selective/differential medium for culturing environmental strains of the group (BFG). This goal was achieved by modifying standard commercial Bile Esculin Agar (BBE Agar). Bile Esculin Agar was combined with substances that inhibit the growth of non-BFG bacteria, mostly and . Read More

Anal Methods 2022 May 12. Epub 2022 May 12.

School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China.

The main production area of HClO and NO is the mitochondria and has modulatory effects on multiple human diseases. Simultaneous detection of signaling molecules such as HClO and NO is an important approach for exploring the complex relationship between HClO and NO in mitochondria. However, most probes can detect only one species or are unable to complete the monitoring of HClO and NO in the NIR channel. Read More

Anal Methods 2022 May 12. Epub 2022 May 12.

College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, China.

Chromatographic fingerprinting provides effective technical means for quality evaluation of traditional Chinese medicine. In this work, a novel multi-wavelength fusion column fingerprint was obtained by intelligent selection of chromatographic peaks from different wavelengths, which displayed the maximum peak area information under the optimal wavelength at the same retention time. Here, the root was selected as a sample. Read More

Anal Methods 2022 May 12. Epub 2022 May 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

Spinosad (SPI) and spinetoram (Et-SPI) are currently among the most popular new insecticides because of their high efficiency and low toxicity. However, excessive residues in food still pose a potential risk to public health. Therefore, it is necessary to strengthen residue monitoring of the two insecticides based on a simple and rapid method. Read More

Anal Methods 2022 May 12. Epub 2022 May 12.

School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, P. R. China.

African swine fever is an acute, severe and highly contagious infectious disease caused by African swine fever virus (ASFV), posing a huge threat to the global swine industry. Rapid and accurate diagnostic methods are of great significance for the effective prevention and control of ASFV transmission. In this work, we established and evaluated a graphene oxide-based accelerated strand exchange amplification (GO-ASEA) method for rapid, highly sensitive, and quantitative detection of ASFV. Read More

Anal Methods 2022 May 12. Epub 2022 May 12.

NANOMISENE Laboratory, LR16CRMN01, Centre for Research on Microelectronics and Nanotechnology CRMN of Sousse Technopole, B.P. 334, Sahloul, Sousse, 4054, Tunisia.

In this work, a novel, cost-effective, and eco-friendly electrochemical (EC) nanosensor was fabricated for the simultaneous detection of daptomycin (DAP) and meropenem (MEROP). EC methods have been developed for the determination of antibiotics. In this context, green synthesized copper nanoparticles (CuNPs) using plant extract were used as electrode modifiers. Read More

Anal Methods 2022 May 11. Epub 2022 May 11.

Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P. O. Box 676, São Carlos, SP, 13560-970, Brazil.

The present study reports the synthesis and characterization of hydrophobic deep eutectic solvents (HDES) based on fatty acids and tetrabutylammonium bromide (TBAB) or 1-octanol using Fourier transform infrared spectroscopy, and the analysis of the physicochemical properties (viscosity, density, electrical conductivity, and water content) of these solvents. A carbon paste electrode modified with 6.0% (m/m) decanoic acid and TBAB-based HDES was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. Read More

Anal Methods 2022 May 11. Epub 2022 May 11.

Physical Sciences Department, MacEwan University, 10700-104 Avenue, Edmonton, AB, T5J 4S2, Canada.

Aflatoxin B1 (AFB1) has been identified as one of the most potent naturally occurring carcinogens with high toxicity. The maximum permissible levels of total aflatoxin contamination in food products are limited to 10-15 μg kg, as established by the Codex Alimentarius Commission. The widespread occurrence of AFB1 in the food chain identifies them as significant agricultural contaminants of global concern. Read More

Anal Methods 2022 May 10. Epub 2022 May 10.

School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China.

In this work, MoS nanosheets were synthesized by the chemical exfoliation method and then modified with a thiol-terminated aptamer a simple thiol functionalization route. The as-made nanomaterial was characterized by UV-vis absorption spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and transmission electron microscopy. By integrating the advantages of MoS nanosheets and the recognition ability of aptamers, the functionalized nanomaterial has been successfully employed for simultaneous enrichment and analysis of sulfadimethoxine by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Read More

Anal Methods 2022 May 10. Epub 2022 May 10.

Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China.

Herein, we report the preparation of a gold nanoparticle-thioglycolic acid ([email protected]) suspension for the label-free colorimetric detection of the organophosphorus pesticide chlorpyrifos. This colorimetric sensor was synthesized by a one-pot method, providing thioglycolic acid (TGA)-modified gold nanoparticles with a negatively charged surface. The formation of [email protected] was confirmed by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

Key Lab of Smart Agriculture System Integration Research, Ministry of Education of China, China Agricultural University, Beijing, 100083, China.

In this research, a novel hydroxyl functionalized covalent organic framework (COF) with fluorescence properties was rationally designed based on the reaction between 2,5-dihydroxy-terephthalic-dihydrazide (DHTPz) and 1,3,5-benzenetricarboxaldehyde (Bt) for Zn detection. The prepared DHTPz-Bt exhibited strong fluorescence, while the apparent quenching of fluorescence was observed after the introduction of Zn. Meanwhile, DHTPz-Bt exhibited high sensitivity and promising selectivity during the detection of Zn. Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

Université de Tours, Faculté de pharmacie, EA 6295 Nanomédicaments et Nanosondes, 31 Avenue Monge, Tours 37200, France.

Attenuated total reflectance-infrared spectroscopy (ATR-IR) coupled with partial least squares regression (PLSR) was evaluated as a rapid, label free and cost-effective tool to quantify water content in extracts obtained from spirulina wet biomass using a glucose glycerol natural deep eutectic solvent (NADES). NADESs are green, renewable and biodegradable solvents with unique properties outcompeting existing organic solvents, for instance, for plant or biomass extraction. The properties of NADESs depend critically on their water concentration, and therefore, it is essential to develop methods to monitor it, to ensure optimal extraction efficiency and experimental repeatability to achieve a better standardization of extraction protocols. Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, Hangzhou Dianzi University, Hangzhou, 310018, China.

An electrochemical biosensor based on few-layer molybdenum disulfide (MoS) nanosheets was fabricated for the highly sensitive detection of tumor marker circulating tumor DNA (ctDNA) in this paper. The MoS nanosheets with few layers were prepared by the shear stripping. Compared with the mechanical stripping method and the lithium ion intercalation method, this method is simpler to operate, and the prepared MoS nanosheets had good electrochemical activity. Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu, P. R. China.

Nanoparticles, which have unique properties, have attracted growing attention in enantiomeric separation nowadays. In this paper, an L-cysteine functionalized gold nanoparticle (L-Cys-GNP) based capillary column was prepared and applied in separating drug enantiomers in capillary electrochromatography (CEC) with lactobionic acid (LA) as a chiral selector. Compared with bare fused-silica capillary columns, the capillary columns modified with L-Cys-GNPs showed excellent chiral separation performance. Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

Pharmaceutical College, Guangxi Medical University, Nanning 530021, China.

Aristolochic acid I (AAI) is one of the nephrotoxic derivatives present in genera and . Although some detection strategies for monitoring AAI have been reported, the application of these methods is limited because they involve tedious preparation and require professional operation. In this work, bovine serum albumin (BSA) has been introduced as a reducing agent and stabilizing agent to synthesize gold nanoclusters with strong red fluorescence for the rapid and effective detection of AAI. Read More

Anal Methods 2022 May 9. Epub 2022 May 9.

School of Laboratory Medicine, Wannan Medical College, Wuhu, 241000, P. R. China.

A flap endonuclease 1 (FEN 1)-assisted swing arm DNA walker was constructed to achieve the signal amplification detection of ctDNA. The MB-labeled hairpin DNA was designed as the track and a long swing-arm DNA strand as the capture probe. The introduction of ctDNA unlocked a helper hairpin DNA, which could be captured to form the DNA duplex walker with the capture probe, and also activated the catalyst hairpin assembly. Read More

Anal Methods 2022 May 6. Epub 2022 May 6.

The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.

BRAF V600E mutation is a single-nucleotide variation (SNV) that is widely found in various cancers and has been demonstrated to have a strong association with the prognosis and development of some diseases. Thus, we developed a strategy based on rolling circle amplification (RCA) and CRISPR/Cas14a to meet the great need for detecting highly specific BRAF V600E mutation in fine-needle biopsy samples. In this study, a padlock probe was designed to recognize and trigger subsequent ligase chain reactions (LCR). Read More

Anal Methods 2022 May 6. Epub 2022 May 6.

Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

Direct synthesis of carbon dots on uniform mesoporous nanospheres is an ideal way to impart fluorescence properties to the nanomaterials and retain its original uniformity. Carbon dot-based nanospheres with high quantum yield (aqueous solution, 89.3%) were synthesized by the one-step hydrothermal treatment of sodium citrate and dendritic silica spheres grafted with -β-(aminoethyl)-γ-aminopropyltrimethoxysilane. Read More

Anal Methods 2022 May 6. Epub 2022 May 6.

College of Chemistry and Chemical Engineering, College of Guangling, Yangzhou University, Yangzhou, 225002, China.

An effective method based on an imidazole ionic liquid functionalized ZIF-67 molecularly imprinted solid-phase extractant ([email protected][Bmim][Br]@MIP) coupled with high performance liquid chromatography (HPLC) for the separation/analysis of bisphenol A (BPA) was established. [email protected][Bmim][Br]@MIP was characterized by FTIR, XRD, SEM, TEM and BET. Several important factors, such as pH, amount of extractant, extraction time, and types of eluents were investigated in detail. Read More

Anal Methods 2022 May 5. Epub 2022 May 5.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 610031, China.

Surface-Enhanced Raman Scattering (SERS) has been widely used in pH sensing. However, SERS sensors capable of stably analysing pH under highly alkaline conditions are still scarce. In this work, a SERS pH sensor employing Alizarin Yellow R as the molecular probe was carefully developed for strong alkaline solutions. Read More

Anal Methods 2022 May 4. Epub 2022 May 4.

Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, Hunan, China.

In this paper, a peroxyoxalate chemiluminescence (CL) recovery system based on the interaction of N-doped graphene oxide nanosheets (N-GONs) and an oligopeptide for copper(II) ion detection has been reported. N-GONs as an excellent CL enhancer are prepared by the hydrothermal method using citric acid and ammonia, and the morphology and structure are characterized in detail by TEM, XPS, FT-IR and UV/vis, In the bis(2,4,6-trichlorophenyl)oxalate (TCPO) and hydrogen peroxide (TCPO + HO) CL reaction system, the addition of N-GONs gives a remarkable CL emission, which can be quenched by an oligopeptide composed of ten amino acid residues due to the interaction between the N-GON plane and the oligopeptide strand. While in the presence of copper(II) ion, the quenched CL is recovered gradually along with the addition of copper(II) ion in the system. Read More

Anal Methods 2022 May 4. Epub 2022 May 4.

Bionanotechnology and Sustainable Laboratory, Department of Biological Sciences, School of Engineering and Applied Sciences, SRM University-AP, Amaravati-522503, India.

Blood group analysis has evolved from conventional "test-tube" to ingenious "lab-on-a-chip" micro/paper-fluidic devices for identifying blood phenotypes. Despite the rapid and economical fabrication of these devices, they require Whatman paper that is obtained by cutting down trees and plastic usage involving complex and sophisticated facilities, making scalable manufacturing laborious and expensive. Most importantly, deforestation and plastic incineration pose great threats to the biotic and abiotic environments. Read More

Anal Methods 2022 May 4. Epub 2022 May 4.

WestCHEM, Department of Pure and Applied Chemistry and CPACT, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.

Process applications of mid-infrared (MIR) spectrometry may involve replacement of the spectrometer and/or measurement probe, which generally requires a calibration transfer method to maintain the accuracy of analysis. In this study, direct standardisation (DS), piecewise direct standardisation (PDS) and spectral space transformation (SST) were compared for analysis of ternary mixtures of acetone, ethanol and ethyl acetate. Three calibration transfer examples were considered: changing the spectrometer, multiplexing two probes to a spectrometer, and changing the diameter of the attenuated total reflectance (ATR) probe (as might be required when scaling up from lab to process analysis). Read More

Anal Methods 2022 May 4. Epub 2022 May 4.

Laboratório de Eletroquímica e Nanotecnologia Aplicada, Instituto de Ciência e Tecnologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rodovia MGT 367, Km 583, 5000, Alto da Jacuba, Diamantina, MG 39100-000, Brazil.

Herein, a so-called carbon nanotube (CNT) electrode was printed in on a paper substrate using the handwriting technique and carbon nanotube ink in a marker pen to print the working electrode, graphite pencil to print the counter electrode and graphite/silver nanoparticle (AgNP) ink in a rollerball pen to print the quasi-reference electrode. The carbon nanotube electrode was characterized scanning electron microscopy. The electrode was optimized based on the type of paper, hydrophobic barrier and number of layers. Read More

Anal Methods 2022 May 4. Epub 2022 May 4.

State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.

A fluorescence detection method based on quantum dot-aptamer-graphene oxide probes (QD-Apt-GO) was developed to detect β-lactoglobulin (β-LG) in foods. When β-LG was present in the samples, it specifically bound to the aptamer, inhibiting the binding of probes to graphene oxide (GO), and the fluorescence of the probes could be detected. When β-LG was not present, the probes could bind to GO through π-π stacking, and the fluorescence was consequently quenched. Read More

Anal Methods 2022 May 3. Epub 2022 May 3.

University of Strathclyde, Department of Pure and Applied Chemistry, George Street, Glasgow, G1 1RD, UK.

Vibrational spectroscopic chemical imaging is an important tool in the pharmaceutical industry for characterising the spatial distribution of components within final drug products. The applicability of these techniques is currently limited by the long data acquisition times required to obtain high-definition chemical images of a sample surface. Advancements in quantum cascade laser (QCL) technology have provided an exciting new opportunity for infrared (IR) imaging. Read More