DNA Microviscosity Characterization with Particle Diffusometry for Downstream DNA Detection Applications.
- Katherine Noel Clayton,
- Gregory D Berglund,
- Jacqueline C Linnes,
- Tamara L Kinzer-Ursem,
- Steven T Wereley
Anal Chem 2017 Nov 17. Epub 2017 Nov 17.
Analytical characterization of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid-based pharmaceutical development, and nucleic acid-based biosensor device design. However, most viscosity characterization methods require large sample volumes and destructive testing. In contrast, particle diffusometry, permits in situ analysis of DNA microviscosity with short measurement times (8 seconds) using small volumes (< 3L) which are compatible with DNA preparatory procedures. Read More