Search our Database of Scientific Publications and Authors

I’m looking for a

    541 results match your criteria Analytical Cellular Pathology [Journal]

    1 OF 11

    Use of nuclear morphometry characteristics to distinguish between normal and abnormal cervical glandular histologies.
    Anal Cell Pathol 2003 ;25(4):193-200
    Department of Statistics, Rice University, P.O. Box 1892, Houston, TX 77005, USA.
    This is a methodological study exploring the use of quantitative histopathology applied to the cervix to discriminate between normal and cancerous (consisting of adenocarcinoma and adenocarcinoma in situ) tissue samples. The goal is classifying tissue samples, which are populations of cells, from measurements on the cells. Our method uses one particular feature, the IODs-Index, to create a tissue level feature. Read More

    Application of multilevel models to morphometric data. Part 2. Correlations.
    Anal Cell Pathol 2003 ;25(4):187-91
    Department of Pathology, School of Medicine, University of Graz, Austria.
    Multilevel organization of morphometric data (cells are "nested" within patients) requires special methods for studying correlations between karyometric features. The most distinct feature of these methods is that separate correlation (covariance) matrices are produced for every level in the hierarchy. In karyometric research, the cell-level (i. Read More

    Quantitation of monoclonal plasma cells in bone marrow biopsies in plasma cell dyscrasia.
    Anal Cell Pathol 2003 ;25(4):167-71
    Haematology Department, Belfast City Hospital, Belfast, BT9 7AB, UK.
    Direct measurement of monoclonal plasma cell mass in bone marrow biopsies may be a useful parameter to establish in plasma cell dyscrasia. In this study monoclonal plasma cells/mm in light chain immunoglobulin immunostained archival bone marrow sections from 22 patients in whom a diagnosis of multiple myeloma (MM) had been excluded but who had monoclonal proteins were counted by two observers at light microscopic level. There was good correlation between the counts of the two observers. Read More

    Earliest detection of oral cancer using non-invasive brush biopsy including DNA-image-cytometry: report on four cases.
    Anal Cell Pathol 2003 ;25(4):159-66
    Department of Oral, Maxillofacial and Facial Plastic Surgery, University of Leipzig, Nürnberger Strasse 57, D-04103 Leipzig, Germany.
    Objective: We describe four patients presenting early oral cancers, detected cytologically on non-invasive brush biopsies including DNA-image cytometry as an adjunctive method before histology on scalpel biopsies confirmed the evidence of malignancy.

    Methods: Brush biopsies were performed and smears thereof investigated cytologically. After Feulgen restaining, DNA-measurements were performed using a DNA-Image-Cytometer. Read More

    Correlation of grade of urothelial cell carcinomas and DNA histogram features assessed by flow cytometry and automated image cytometry.
    Anal Cell Pathol 2003 ;25(3):147-53
    Department of Pathology, SIR Hospital, Stavanger, Norway.
    Objective: To analyse how DNA ploidy and S-phase fraction (SPF) by flow cytometry (FCM) and an optimised fully automatic DNA image cytometer (ICM) correlate with grade in TaT1 urothelial cell carcinomas (UC) of the urinary bladder.

    Materials And Methods: Two-hundred-and twenty-eight consensus cases were analysed. Single cell suspensions were stained (DAPI for FCM, Feulgen for ICM). Read More

    Diagnostic value of nucleolar organizer regions (AgNORs) in brush biopsies of suspicious lesions of the oral cavity.
    Anal Cell Pathol 2003 ;25(3):139-46
    Department of Oral, Maxillofacial and Facial Plastic Surgery, University of Leipzig, Nürnberger Strasse 57, D-04103 Leipzig, Germany.
    Objective: The aim of this retrospective study was to report on the diagnostic accuracy of AgNOR-analysis as an adjunctive diagnostic tool of conventional oral exfoliative cytology taken from suspicious lesions in our clinic.

    Study Design: Cytological diagnoses obtained from brush biopsies of macroscopically suspicious lesions of the oral mucosa from 75 patients (final diagnoses: 53 histologically proven squamous cell carcinomas, 11 leukoplakias and other inflammatory oral lesions) and from 11 patients with normal mucosa as a negative control group were compared with histological and/or clinical follow-ups. Five smears were doubtful and seven suspicious for tumor cells in the cytologic report. Read More

    Risk biomarker assessment for breast cancer progression: replication precision of nuclear morphometry.
    Anal Cell Pathol 2003 ;25(3):129-38
    Cancer Imaging, BC Cancer Research Center, Vancouver, Canada.
    Nuclear morphometry is a method for quantitative measurement of histopathologic changes in the appearance of stained cell nuclei. Numerous studies have indicated that these assessments may provide clinically relevant information related to the degree of progression and malignant potential of breast neoplasia. Nuclear features are derived from computerized analysis of digitized microscope images, and a quantitative Feulgen stain for DNA was used. Read More

    Cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity.
    Anal Cell Pathol 2003 ;25(3):115-22
    Laboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20-70-IFR 53 Biomolecules), 51100 Reims, France.
    In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex-FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug-resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. Read More

    DNA amplifications and aneuploidy, high proliferative activity and impaired cell cycle control characterize breast carcinomas with poor prognosis.
    Anal Cell Pathol 2003 ;25(3):103-14
    Department of Oncology-Pathology, Karolinska Institute, Cancer Center Karolinska, R8:04, SE-171 76, Stockholm, Sweden.
    In order to explore whether specific cytogenetic abnormalities can be used to stratify tumors with a distinctly different clinical course, we performed comparative genomic hybridization (CGH) of tumors from patients who were diagnosed with metastatic disease after an interval of less than 2 years or who remained free from distant metastases for more than 10 years. All patients presented with distant metastases after mastectomy indicating that none of the patients in this study was cured and free of remaining tumor cells. Tumors in the group of short-term survivors showed a higher average number of chromosomal copy alterations compared to the long-term survivors. Read More

    In vitro model for studying malignancy associated changes.
    Anal Cell Pathol 2003 ;25(2):95-102
    Cancer Imaging Department, British Columbia Cancer Agency, Vancouver, Canada.
    Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non-small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC-like phenomenon can be detected in such a system. Read More

    Gain in 1q is a common abnormality in phyllodes tumours of the breast.
    Anal Cell Pathol 2003 ;25(2):89-93
    Departments of Pathology and Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland.
    We studied DNA copy number changes by CGH and allelic imbalance (AI) on 3p by LOH analysis on 22 phyllodes tumours (PT) of the breast in order to gain insight into the genetic basis of tumour progression in PT. Copy number changes were observed in 14 cases (63%). Gain in 1q with 1q21-23 as the minimal overlapping area was seen in 12 cases (55%). Read More

    DNA extraction from bronchial aspirates for molecular cytology: which method to take?
    Anal Cell Pathol 2003 ;25(2):83-8
    Institute of Cytopathology, Heinrich-Heine-University, Moorenstrasse 5, D-40225 Düsseldorf, Germany.
    Objective: To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to preserve bronchial secretions with high quality genomic DNA as well as to compare different DNA extraction methods.

    Methods: DNA was extracted from 45 bronchial aspirates by four different extraction protocols. Read More

    Expression of the cell adhesion molecule CD146/MCAM in non-small cell lung cancer.
    Anal Cell Pathol 2003 ;25(2):77-81
    Institute of Pathology, Charité University Hospital, 10117 Berlin, Germany.
    Expression of MCAM is observed in a variety of human malignancies. We aimed to determine the rate of MCAM expression in our non-small cell lung cancer (NSCLC) collection and to clarify its correlation with clinicopathological parameters and patient survival. 85 NSCLC were analysed immunohistochemically using a monoclonal MCAM antibody (clone N1238) on an NSCLC tissue micro array. Read More

    An original approach for quantification of blood vessels on the whole tumour section.
    Anal Cell Pathol 2003 ;25(2):63-75
    GRECAN, Equipe Universitaire d'Accueil 1772, Centre F. Baclesse, Route de Lion sur Mer, F-14076 Caen, France.
    Relative abundance of tumour angiogenesis has been shown to be of clinical relevance in cancers of various locations such as the ovary. Nevertheless, several problems are encountered when quantifying tumour microvessels: (i) as many other tumour markers, vascularity pattern is often heterogeneous within the tumour mass and even within the same histological section. As a consequence, an adequate acquisition method must be developed for accurate field sampling. Read More

    Analysis of ploidy in hypopharyngeal cancer by laser scanning cytometry on fine needle aspirate biopsies.
    Anal Cell Pathol 2003 ;25(2):51-62
    Department of Otorhinolaryngology, University of Leipzig, Germany.
    Aim: To test laser scanning cytometry (LSC) for the analysis of ploidy in squamous cell carcinoma of the hypopharynx (SCCH) and to develop a routine application for minimal samples such as fine needle aspirate biopsies (FNABs).

    Methods: From 11 individuals 30 FNABs of primary tumors (n=11) and lymphatic metastases of SCCH (n=11) and non-metastatic lymph nodes (n=8) are analyzed by LSC. This microscope based instrument scans the cells after immobilization on a glass slide and after double staining of cytokeratin and DNA. Read More

    Automated detection of working area of peripheral blood smears using mathematical morphology.
    Anal Cell Pathol 2003 ;25(1):37-49
    Centre de Morphologie Mathématique, Ecole des Mines de Paris, Fontainebleau, France.
    The paper presents a technique to automatically detect the working area of peripheral blood smears stained with May-Grünwuald Giemsa. The optimal area is defined as the well spread part of the smear. This zone starts when the erythrocytes stop overlapping (on the body film side) and finishes when the erythrocytes start losing their clear central zone (on the feather edge side). Read More

    A feature set for cytometry on digitized microscopic images.
    Anal Cell Pathol 2003 ;25(1):1-36
    GSF National Research Center for Ecology and Health, Institute of Biomathematics and Biometry, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
    Feature extraction is a crucial step in most cytometry studies. In this paper a systematic approach to feature extraction is presented. The feature sets that have been developed and used for quantitative cytology at the Laboratory for Biomedical Image Analysis of the GSF as well as at the Center for Image Analysis in Uppsala over the last 25 years are described and illustrated. Read More

    Chromosomal regions in prostatic carcinomas studied by comparative genomic hybridization, hierarchical cluster analysis and self-organizing feature maps.
    Anal Cell Pathol 2002 ;24(4-5):167-79
    Department of Pathology, University of Ulm, Ulm, Germany.
    Comparative genomic hybridization (CGH) is an established genetic method which enables a genome-wide survey of chromosomal imbalances. For each chromosome region, one obtains the information whether there is a loss or gain of genetic material, or whether there is no change at that place. Therefore, large amounts of data quickly accumulate which must be put into a logical order. Read More

    Dependence of DNA-histograms on the sampling techniques in fine needle aspirates of the breast.
    Anal Cell Pathol 2002 ;24(4-5):159-65
    Department of Pathology, University of Turku, Finland.
    48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Different sampling strategies were applied. Read More

    Evaluation of prognostic factors following flow-cytometric DNA analysis after cytokeratin labelling: II. Cervical and endometrial cancer.
    Anal Cell Pathol 2002 ;24(4-5):147-58
    Department of Obstetrics and Gynecology, University of Essen, Hufelandstr. 55, D-45122 Essen, Germany.
    In gynecologic oncology valid prognostic factors are necessary to define biologically similar subgroups for analysis of therapeutic efficacy. This study is the first published prospective study concerning prognostic significance of DNA ploidy and S-phase fraction in cervical and endometrial cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC-conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 91 specimens of cervical cancer and 73 samples of endometrial cancer. Read More

    Evaluation of prognostic factors following flow-cytometric DNA analysis after cytokeratin labelling: I. Breast cancer.
    Anal Cell Pathol 2002 ;24(4-5):135-45
    Department of Obstetrics and Gynecology, University of Essen, Hufelandstr. 55, D-45122 Essen, Germany.
    In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study is a prospective study concerning prognostic significance of DNA ploidy and S-phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC-conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Read More

    Flow cytometric bivariate analysis of DNA and cytokeratin in colorectal cancer.
    Anal Cell Pathol 2002 ;24(4-5):113-24
    Finsen Laboratory, Finsen Center, Rigshospitalet, University Hospital, Copenhagen, Denmark.
    Different opinions about flow cytometric estimates of DNA aneuploidy and/or S-phase fraction (SPF) as supplementary prognostic markers in colorectal cancer are to some degree associated with methodology. Using univariate DNA analysis, we have previously investigated the DNA ploidy in colorectal cancer, its heterogeneity within and between tumors and its relation to survival. To improve detection of DNA aneuploid subpopulations and particularly estimation of their SPF's we investigated a method for bivariate DNA/cytokeratin analysis on fine-needle aspirates of 728 frozen biopsies from 157 colorectal tumors. Read More

    Algorithms for cytoplasm segmentation of fluorescence labelled cells.
    Anal Cell Pathol 2002 ;24(2-3):101-11
    Centre for Image Analysis at Uppsala University, Uppsala, Sweden.
    Automatic cell segmentation has various applications in cytometry, and while the nucleus is often very distinct and easy to identify, the cytoplasm provides a lot more challenge. A new combination of image analysis algorithms for segmentation of cells imaged by fluorescence microscopy is presented. The algorithm consists of an image pre-processing step, a general segmentation and merging step followed by a segmentation quality measurement. Read More

    Variations in cell surfaces of estrogen treated breast cancer cells detected by a combined instrument for far-field and near-field microscopy.
    Anal Cell Pathol 2002 ;24(2-3):89-100
    Department Single Cell and Single Molecule Techniques, Institute of Molecular Biotechnology e V, P O Box 100813, D-07708 Jena, Germany.
    The response of single breast cancer cells (cell line T-47D) to 17beta-estradiol (E(2)) under different concentrations was studied by using an instrument that allows to combine far-field light microscopy with high resolution scanning near-field (AFM/SNOM) microscopy on the same cell. Different concentrations of E(2) induce clearly different effects as well on cellular shape (in classical bright-field imaging) as on surface topography (atomic force imaging) and absorbance (near-field light transmission imaging). The differences range from a polygonal shape at zero via a roughly spherical shape at physiological up to a spindle-like shape at un-physiologically high concentrations. Read More

    Chromosomal imbalances are associated with metastasis-free survival in breast cancer patients.
    Anal Cell Pathol 2002 ;24(2-3):77-87
    GSF - Research Center for Environment and Health, Institute of Pathology, D-85764 Neuherberg, Germany.
    Multiple chromosomal imbalances have been identified in breast cancer using comparative genomic hybridization (CGH). Their association with the primary tumors' potential for building distant metastases is unknown. In this study we have investigated 39 invasive breast carcinomas with a mean follow-up period of 99 months (max. Read More

    Genetic alterations in presumptive precursor lesions of breast carcinomas.
    Anal Cell Pathol 2002 ;24(2-3):69-76
    GSF-National Research Center for Environment and Health, Institute of Pathology Neuherberg, Germany.
    The hypothetical multistep model of breast carcinogenesis suggests a transition from normal epithelium to invasive carcinoma via intraductal hyperplasia (without and with atypia) and in situ carcinoma. These presumptive precursor lesions are currently defined by their histological features, and their prognosis is imprecisely estimated from indirect epidemiological evidence. Cytogenetic and molecular-genetic analysis of these lesions give evidence for an accumulation of various genetic alterations during breast tumorigenesis. Read More

    Tissue counter analysis of histologic sections of melanoma: influence of mask size and shape, feature selection, statistical methods and tissue preparation.
    Anal Cell Pathol 2002 ;24(2-3):59-67
    Department of Dermatology, Graz, Austria.
    Background: Tissue counter analysis is an image analysis tool designed for the detection of structures in complex images at the macroscopic or microscopic scale. As a basic principle, small square or circular measuring masks are randomly placed across the image and image analysis parameters are obtained for each mask. Based on learning sets, statistical classification procedures are generated which facilitate an automated classification of new data sets. Read More

    An integrated system for feature evaluation of 3D images of a tissue specimen.
    Anal Cell Pathol 2002 ;24(2-3):47-58
    Bio-Imaging Group, Lawrence Berkley National Laboratory, Berkley, CA 94705, USA.
    In this article we have proposed an integrated system for measurement of important features from 3D tissue images. We propose a segmentation technique, where we combine several methods to achieve a good degree of automation. Important histological and cytological three-dimensional features and strategies to measure them are described. Read More

    Additional techniques in serous effusions.
    Anal Cell Pathol 2002 ;24(1):1-4
    Cytological examination is a valuable diagnostic tool in case of a serous effusion. The first manifestation of malignancy may be an effusion of the pleural, pericardial, or peritoneal cavity, especially in carcinoma of the ovary, or lung, and malignant mesothelioma. In other malignancies effusions may occur in the course of the disease. Read More

    CD10 expression in non-small cell lung cancer.
    Anal Cell Pathol 2002 ;24(1):41-6
    Institute of Pathology, Charité University Hospital, Berlin, Germany.
    CD10 is a cell surface endopeptidase that inactivates various potentially growth stimulatory peptides. In lung cancer cell lines this downregulation has been associated with increased proliferation. Downregulation of CD10 in lung cancer tissue is described, suggesting a potential role in carcinogenesis and a possible use of CD10 as a prognostic marker. Read More

    Telomerase activity levels in the surgical margin and tumour distant tissue of the squamous cell carcinoma of the head-and-neck.
    Anal Cell Pathol 2002 ;24(1):25-39
    Clinic for Oral and Maxillofacial Surgery, Campus Virchow Hospital, Charité, Medical Faculty of the Humboldt University of Berlin, Germany.
    The survival of patients with a head-and-neck squamous cell carcinoma is determined by loco-regional recurrence and second primary carcinomas. As a complement to histopathology, molecular changes of tumour marginal and tumour distant tissue may confirm curative surgical tumour extirpation. We tested telomerase activity with PCR-ELISA kits. Read More

    Expression of multidrug resistance-associated markers, their relation to quantitative pathologic tumour characteristics and prognosis in advanced ovarian cancer.
    Anal Cell Pathol 2002 ;24(1):17-23
    Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands.
    Mean nuclear area has been consistently shown by different researchers to be a strong and independent prognostic factor in advanced ovarian carcinoma. However, the biological background of the prognostic value of nuclear area remains unclear. Others have found that the multidrug-resistance (MDR) related protein LRP has strong prognostic value. Read More

    Diagnostic DNA-flow- vs. -image-cytometry in effusion cytology.
    Anal Cell Pathol 2002 ;24(1):5-15
    Institute of Cytopathology, Heinrich-Heine-University, Moorenstr. 5, D-40225 Düsseldorf, Germany.
    Aims: To determine the sensitivity and specificity of flow- and image-cytometry for the detection of DNA-aneuploidy as a marker for malignant cells in effusions.

    Methods: 200 effusions (80 tumor cell-positive, 74 negative and 46 cytologically equivocal) were stained with DAPI-SR for DNA-flow- and with Feulgen-Pararosaniline for -image-cytometry. They were measured using a PAS-flow-cytometer and an AutoCyte-QUIC-DNA-workstation according to the ESACP consensus reports for DNA-flow- and -image-cytometry, respectively [7,23,29,49]. Read More

    Automatic registration and error detection of multiple slices using landmarks.
    Anal Cell Pathol 2001 ;23(3-4):159-65
    Centre for Image Analysis, Uppsala, Sweden.
    Objectives: When analysing the 3D structure of tissue, serial sectioning and staining of the resulting slices is sometimes the preferred option. This leads to severe registration problems. In this paper, a method for automatic registration and error detection of slices using landmark needles has been developed. Read More

    Automated detection of connective tissue by tissue counter analysis and classification and regression trees.
    Anal Cell Pathol 2001 ;23(3-4):153-8
    Department of Dermatology, University of Graz, Auenbruggerplatz 8, A-8036 Graz, Austria.
    Objective: To evaluate the feasibility of the CART (Classification and Regression Tree) procedure for the recognition of microscopic structures in tissue counter analysis.

    Methods: Digital microscopic images of H & E; stained slides of normal human skin and of primary malignant melanoma were overlayed with regularly distributed square measuring masks (elements) and grey value, texture and colour features within each mask were recorded. In the learning set, elements were interactively labeled as representing either connective tissue of the reticular dermis, other tissue components or background. Read More

    DNA ploidy and S-phase fraction in carcinoma of the gallbladder related to histopathology, number of gallstones and survival.
    Anal Cell Pathol 2001 ;23(3-4):143-52
    Department of Surgery, Danderyd Hospital, Sweden.
    Gallstones are a risk factor for the development of gallbladder cancer. We studied DNA ploidy and cell cycle composition by flow cytometry in archival specimens from 52 gall bladder carcinomas in relation to histopathological grade, tumour stage, gallstone number and survival. 69% of the gallbladder carcinomas showed aneuploidy. Read More

    RNA relocation and persistence of nucleolus-like bodies at mitosis in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17.
    Anal Cell Pathol 2001 ;23(3-4):137-41
    Department of Cell Biology, Institute of Biology, UNICAMP, 13084-971 Campinas, SP, Brazil.
    RNA relocation and the incidence of nucleolus-like bodies accumulated during mitosis were studied cytochemically in benzo[a]pyrene (BP)-transformed human breast epithelial MCF-10F cells after microcell-mediated transfer of normal chromosomes 11 and 17. The changes resulting from the transfer of these two chromosomes in tumorigenic MCF-10F cells (BP1-E cell line) were examined, since alterations in these chromosomes are involved in the expression of the transformed and tumorigenic phenotypes in the MCF-10F cell series. In addition, the frequency of nucleolus-like bodies decreases drastically with transformation and tumorigenicity in MCF-10F cells, thus being conceivable that it would be affected in presence of normal chromosomes 11 or 17. Read More

    Application of neural networks to the classification of pancreatic intraductal proliferative lesions.
    Anal Cell Pathol 2001 ;23(3-4):129-36
    Chair of Pathomorphology Jagiellonian University, Kraków, Poland.
    The aim of the study was to test applycability of neural networks to classification of pancreatic intraductal proliferative lesions basing on nuclear features, especially chromatin texture. Material for the study was obtained from patients operated on for pancreatic cancer, chronic pancreatitis and other tumours requiring pancreatic resection. Intraductal lesions were classified as low and high grade as previously described. Read More

    Ovarian carcinoma and serous effusions. Changing views regarding tumor progression and review of current literature.
    Anal Cell Pathol 2001 ;23(3-4):107-28
    Department of Pathology, The Norwegian Radium Hospital University of Oslo, Montebello, N-0310 Oslo, Norway.
    Carcinoma of the ovary is the leading cause of death from gynecological cancer in western countries. Ovarian carcinoma is commonly associated with the accumulation of fluid containing malignant cells in the peritoneal, and not infrequently in the pleural cavity. The differentiation of these cells from reactive mesothelial cells is at times difficult. Read More

    Prognostic significance of DNA cytometry in carcinoma of the uterine cervix FIGO stage IB and II.
    Anal Cell Pathol 2001 ;23(3-4):97-105
    Institute of Cytopathology, Heinrich-Heine-University, Moorenstrasse 5, D-40225 Düsseldorf, Germany.
    Objective: To assess the prognostic value of DNA-image cytometry in cervical carcinoma of the uterus and its relation to other established prognostic factors.

    Study Design: The study included 116 cases of cervical carcinoma FIGO stages IB and II which were treated with radical abdominal hysterectomy. The median follow-up was 55 months (range 1-162 months). Read More

    Fourth updated ESACP consensus report on diagnostic DNA image cytometry.
    Anal Cell Pathol 2001 ;23(2):89-95
    Dresden-Friedrichstadt General Hospital, Dresden, Germany.
    A task force of experts in the field of diagnostic DNA image cytometry, invited by the ESACP, and further scientists or physicians revealing experience in that diagnostic procedure (names are given in Addendum A), agreed upon the following 4th updated Consensus Report on Standardised Diagnostic DNA Image Cytometry during the 7th International Congress of that society in Caen, 2001. This report is based on the three preceding ones [6,14,17]. It deals with the following items:- Critical review and update of the definitions given in the 1997 Consensus Update;- Review and detailed description of basic terms, principles and algorithms for diagnostic interpretation;- Recommendations concerning diagnostic or prognostic applications in specific fields of tumour pathology. Read More

    Prognostic classification of early ovarian cancer based on very low dimensionality adaptive texture feature vectors from cell nuclei from monolayers and histological sections.
    Anal Cell Pathol 2001 ;23(2):75-88
    Department of Informatics, University of Oslo, P.O.Box 1080 Blindern, N-0316 Oslo, Norway.
    In order to study the prognostic value of quantifying the chromatin structure of cell nuclei from patients with early ovarian cancer, low dimensionality adaptive fractal and Gray Level Cooccurrence Matrix texture feature vectors were extracted from nuclei images of monolayers and histological sections. Each light microscopy nucleus image was divided into a peripheral and a central part, representing 30% and 70% of the total area of the nucleus, respectively. Textural features were then extracted from the peripheral and central parts of the nuclei images. Read More

    Topical mitomycin C and radiation induce conjunctival DNA-polyploidy.
    Anal Cell Pathol 2001 ;23(2):65-74
    Department of Ophthalmology, Heinrich-Heine University, Düsseldorf, Germany.
    Introduction: Atypical cell changes often occur following treatment of premalignant or malignant conjunctival neoplasias with topical mitomycin C (MMC) and/or radiation. These reactive, non-neoplastic alterations of the conjunctival epithelium can be a differential diagnostic problem. Our aim was to investigate changes in the nuclear DNA-distribution of conjunctival epithelial cells after MMC- and radiation therapy by DNA-image-cytometry. Read More

    1 OF 11