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Anal Biochem 2022 May 24:114740. Epub 2022 May 24.

Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, 100144, China. Electronic address:

Meningococcal disease caused by Neisseria meningitidis remains a major global public health concern. Serogroup A, B, C and W135 were the major disease-causing serogroups. It is vital to timely and efficiently detect and differentiate these four serogroups. Read More

Anal Biochem 2022 May 23:114748. Epub 2022 May 23.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 401331, China. Electronic address:

A simple and time-saving colorimetric method was developed to quantify sulfonamides (SAAs) in milk via inhibition of the human carbonic anhydrase II (hCAII)-like activity of ZIF-8 that can hydrolyze p-nitrophenyl acetate (pNPA) to p-nitrophenol (pNP), following the color change from yellow to colorless. Effects of different reaction conditions, including pH, temperature, amount of ZIF-8, and incubation time, were investigated. The value of Michaelis-Menten constant (K) is measured to be 0. Read More

Anal Biochem 2022 May 23:114719. Epub 2022 May 23.

Department of Chemistry, Tsinghua University, Beijing, 100084, China. Electronic address:

Monitoring clinical biomarkers, such as testosterone in serum, is important for disease assessment. Due to the very low concentration of testosterone in serum, we have developed a new strategy for its enrichment in serum samples by magnetic molecularly imprinted polymers (MMIPs) technology and detection by nano-electrospray ionization mass spectrometry (Nano-ESI-MS). Testosterone was selectively extracted and enriched by the imprinted polymers on the surface of magnetic particles and the complex matrix was eliminated from the serum. Read More

Anal Biochem 2022 May 21:114746. Epub 2022 May 21.

School of Science, Xi'an Polytechnic University, Xi'an, 710048, PR China.

N4-methylcytosine (4mC) is an important and common methylation which widely exists in prokaryotes. It plays a crucial role in correcting DNA replication errors and protecting host DNA against degradation by restrictive enzymes. Hence, the accurate identification for 4mC sites is greatly significant for understanding biological functions and treating gene diseases. Read More

Anal Biochem 2022 May 21:114728. Epub 2022 May 21.

University of Lethbridge, Dept. of Chemistry and Biochemistry, Lethbridge, Alberta, Canada; AUC Solutions, LLC, Houston, TX, USA; University of Montana, Dept. of Chemistry, Missoula, MT, USA. Electronic address:

Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic hydrodynamic information content of AUC and provides access to a wide range of new applications for biopolymer characterization, and is poised to become an essential analytical tool to study macromolecular interactions. It adds an orthogonal spectral dimension to the traditional hydrodynamic characterization by exploiting unique chromophores in analyte mixtures that may or may not interact. Read More

Anal Biochem 2022 May 21:114745. Epub 2022 May 21.

National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

Expression of proteins in bacterial host cells, particularly E.coli, has gained much attention in recent years. Low expression outcome is the main technical drawback associated with this procedure, further restricting its largescale application in industry. Read More

Anal Biochem 2022 May 18;652:114725. Epub 2022 May 18.

Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, 88040900, SC, Brazil. Electronic address:

A simple and rapid methodology was developed using hollow fiber membrane microporous and a 96-well plate system for a high throughput multiclass determination of endocrine disruptors in human urine (diclofenac, diazepam, carbamazepine, ibuprofen, naproxen, carbofuran, methyl parathion, 17-α-ethynyl estradiol, bisphenol A and benzophenone). The quantification and detection of the chemicals were carried out by an HPLC-diode array detector. The fixed conditions for carrying out the method optimization were 1. Read More

Anal Biochem 2022 May 18;652:114738. Epub 2022 May 18.

Education Organization of Guilan Province, Farhang Square, P.O. Box: 41849-83111, Rasht, Iran.

Metal ion-DNA interactions are important in nature as they often change the genetic material's structure and function. In this work, new Tb complex of TbCl (tris(8-hydroxyquinoline-5-sulfonic acid) terbium) (Tb(QS)) was used as an electrochemical indicator for investigation of DNA-metal interaction and then a new DNA biosensor was designed using this complex. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the interaction of Tb(QS) with double-stranded DNA (ds-DNA). Read More

Anal Biochem 2022 May 18;651:114737. Epub 2022 May 18.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, PR China; West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, Sichuan, PR China. Electronic address:

As Helicobacter pylori (H. pylori) is closely related to the occurrence of gastric diseases such as chronic gastritis, peptic ulcer, and gastric cancer, early detection of H. pylori is an urgent need. Read More

Anal Biochem 2022 May 16:114726. Epub 2022 May 16.

Key Laboratory of Applied Chemistry, Department of Applied Chemistry, Yanshan University, Qinhuangdao, 066004, PR China. Electronic address:

Bimetallic nanomaterials with various dimensions have been successfully explored in electrochemical biosensor to detect the carbamate pesticide. One-dimensional bimetallic nanomaterials with mesoporous, which possess bigger electrochemical active area, more catalytic active sites and faster electron transmission efficiency, may have excellent performance in electrochemical biosensor, but have been rarely reported. In order to confirm this hypothesis, one-dimensional PdRh alloy mesoporous nanotubes were prepared and applied as a platform for carbamate pesticide electrochemical detection. Read More

Anal Biochem 2022 May 17:114736. Epub 2022 May 17.

Department of Anatomy, Histology Forensic Medicine, Sapienza University of Rome, Rome, Italy.

Leukemia often initiates following dysfunctions in hematopoietic stem cells lineages. Various types of leukemia, including acute lymphoblastic leukemia (ALL), chronic myelogenous leukemia (CML), acute promyelocytic leukemia (APL), and human T-cell leukemia/lymphoma virus type 1 (HTLV-1) can thus call for different diagnosis and treatment options. One of the most important subjects in leukemia is the early detection of the disease for effective therapeutic purposes. Read More

Anal Biochem 2022 May 14:114727. Epub 2022 May 14.

Department of Chemistry and Biochemistry, Utah State University, Logan, UT, 84322-0300, USA. Electronic address:

The two constitutional isomers of naphthyl phosphate have different steric properties, analogous to those of phosphotyrosine versus phosphoserine/threonine within a peptide or protein. The ratios of their respective rates of hydrolysis, assayed by measuring rates of inorganic phosphate release, have been used to probe the steric requirements around the active sites of many phosphatases in the literature. We report an NMR-based competitive method that is simpler to execute and has other advantages. Read More

Anal Biochem 2022 May 13;650:114721. Epub 2022 May 13.

Cellular and Structural Physiology Institute (CeSPI), Nagoya University, Nagoya, 464-8601, Japan; CeSPIA Inc., Tokyo, 100-0004, Japan. Electronic address:

Accurate serologic evaluation of autoantibodies in patients with autoimmune diseases is critical. In the present study, we established a live cell-based assay for simultaneous detection of multiple autoantibodies in a single serum sample. Autoantibody seropositivity was determined by 3-color flow cytometry using live Chinese hamster ovary cells transiently expressing a target protein of interest fused to enhanced green fluorescent protein and labeled with Alexa Fluor 647 and Hoechst 33342. Read More

Anal Biochem 2022 May 13;650:114724. Epub 2022 May 13.

Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.

High-potential iron-sulfur proteins (HiPIPs) from extremely acidophilic chemolithotrophic non-photosynthetic Acidithiobacillus commonly play a crucial role in ferrous or sulfurous biooxidation. Acidithiobacillus exhibit important industrial applications for bioleaching valuable metals from sulfide ores. In this study, two HiPIP genes from thermophilic Acidithiobacillus caldus SM-1 were cloned and successfully expressed, and their proteins were purified. Read More

Anal Biochem 2022 May 12;650:114707. Epub 2022 May 12.

School of Engineering and Technology, Central Queensland University, North Rockhampton, QLD, Australia.

Cancer is one of the most dangerous diseases in the world that often leads to misery and death. Current treatments include different kinds of anticancer therapy which exhibit different types of side effects. Because of certain physicochemical properties, anticancer peptides (ACPs) have opened a new path of treatments for this deadly disease. Read More

Anal Biochem 2022 May 11:114710. Epub 2022 May 11.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, China; International Joint Research Center for Animal Immunology, Zhengzhou, 450002, Henan, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, 450002, Henan, China. Electronic address:

Peanut oil is a basic food raw material in life. However, aflatoxin contamination in peanut oil is considered to be one of the most serious food safety problems in the world. Based on AuNPs/Zn/[email protected] composite, a simple, efficient and sensitive electrochemical immunosensor was developed to detect aflatoxin B1 (AFB1) in peanut oil. Read More

Anal Biochem 2022 May 12;650:114723. Epub 2022 May 12.

Department of Science and Environment, Enhanced Perioperative Oncology (EPeOnc) Consortium, Roskilde University, Universitetsvej 1, 4000, Roskilde, Denmark. Electronic address:

Background: Methods measuring cell proliferation and adhesion are widely used but each hold limitations. We, therefore, introduce novel methods for measuring cell proliferation and adhesion based on CRISPR-modified cancer cell lines secreting luciferase to the growth media.

Materials And Methods: Using CRISPR genome editing, we generated stable luciferase-secreting LS174T, HCT 116, Caco-2, and PANC-1 cell lines. Read More

Anal Biochem 2022 May 10;650:114711. Epub 2022 May 10.

Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, PR China; Beijing Institute of Radiation Medicine, Beijing, 100850, PR China. Electronic address:

Background: TaqMan probe-based real-time PCR (qPCR/RT-qPCR) has been widely used in various fields because of its high sensitivity and specificity. However, TaqMan probes are associated with a relatively higher background signal, and hence negatively affect the detection results.

Methods: Double-stranded probes (DSPs) were designed for the high sensitive detection of hepatitis B virus (HBV) DNA and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA using qPCR/RT-qPCR. Read More

Anal Biochem 2022 May 10;650:114712. Epub 2022 May 10.

Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, 200 First St. SW, Rochester, MN, 55905, USA. Electronic address:

The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by regions of fixed sequence, a configuration essential for in vitro selection experiments. Here we describe the results of experiments revealing why conventional qPCR methods can fail to allow automated analysis in such cases, and simple solutions to this problem. Read More

Anal Biochem 2022 Jul 7;649:114722. Epub 2022 May 7.

Medical laboratory of Shenzhen Luohu Hospital Group, 518003, China. Electronic address:

Autoantibodies are currently the most robust biomarkers of type 1 diabetes. However, single autoantibody targeted detection is still limited in diabetes diagnosis with poor performance. Here, we develop a multiplexed Array-ELISA assay that can detect five diabetes-related autoantibodies including glutamic acid decarboxylase antibody (GADA), insulinoma antigen 2 antibody (IA-2A), islet cell antibody (ICA), zinc transporter 8 autoantibody (ZnT8-A) and insulin antibody (IAA) simultaneously. Read More

Anal Biochem 2022 May 6;651:114720. Epub 2022 May 6.

Chemistry Department, Faculty of Science, Benha University, Benha, Egypt. Electronic address:

A novel selective and precise optical thin film sensor reliant on the incorporation of synthesized 2-(2'-hydroxynaphthylazo)-benzothiazole (HNABT) as a selective ionophore into the nonporous of a clear glass like material via the sol-gel process is examined for its ability to assess Mn(II) ions in aqueous solutions. The sensor was constructed by spin-coating prepared sol onto a glass plate and the influence of sonication time on immobilization of HNABT into silica matrix was demonstrated through calculation of leaching percentage. The results designated that a sonication time of 25 min is the optimum to accomplish more stable thin films without fluctuation in sensitivity and response time of the introduced sensor for a wide period. Read More

Anal Biochem 2022 Jul 5;649:114691. Epub 2022 May 5.

Center for Biomedical Engineering, School of Engineering, Brown University, 184 Hope Street, Providence, RI, 02912, USA. Electronic address:

The aim of this research is to present a new method to identify and separate target DNA of the same size, in base pairs (bp), into different sizes based on the targeted sequences. This sequence-specific analysis can then be used to evaluate the presence of multiple targeted analytes in a sample without the need for fluorescence detection. This work displays the feasibility of this method using multiple different 150 bp target sequences separated via microfluidic electrophoresis into 230 bp to 330 bp peaks. Read More

Anal Biochem 2022 Jul 4;649:114698. Epub 2022 May 4.

Department of Science and Environment, Roskilde University, Universitetsvej 1, Roskilde, Denmark. Electronic address:

Prostate cancer (PCa) is the second most common cancer in men and one of the leading causes of cancer-related deaths. Early detection is the key to successful treatment and provides the greatest chance to cure the patient. Currently, early detection involves screening for prostate-specific antigen levels in blood, which is not a tumor-specific biomarker. Read More

Anal Biochem 2022 Jul 29;649:114693. Epub 2022 Apr 29.

Department of Chemistry and Biochemistry, Kent State University, Kent, OH, 44242, USA. Electronic address:

Binding between a ligand and a receptor is a fundamental step in many natural or synthetic processes. In biosensing, a tight binding with a small dissociation constant (K) between the probe and analyte can lead to superior specificity and sensitivity. Owing to their capability of evaluating competitors, displacement assays have been used to estimate K at the ensemble average level. Read More

Anal Biochem 2022 Apr 29:114700. Epub 2022 Apr 29.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Alexandria University, 1 El Khartoum Square, Alexandria, 21521, Egypt.

Sunscreens (SSs) are highly applied all over the world on large areas of human body. Benzophenone chemical group constitute a major part in most SSs. Benzophenones are reported to induce changes in nucleic acids upon UV-irradiation. Read More

Anal Biochem 2022 Jul 30;649:114696. Epub 2022 Apr 30.

Université de Montréal, Département de Médicine, 2900 Edouard Montpetit Blvd, Montréal, Québec, H3T 1J4, Canada; Laboratoire Hépato-Neuro, Centre de recherche du Centre Hospitalier de l'Université de Montréal, 900 Saint Denis St, Montréal, Québec, H2X 0A9, Canada. Electronic address:

Hepatic encephalopathy (HE) is a decline in brain function arising due to liver insufficiency. The liver's diminished capacity to clear ammonia, and the subsequent accumulation of it, is highly implicated in pathogenesis of HE. Ammonia is endogenously generated from the catabolism of amino acids derived from dietary protein intake. Read More

Anal Biochem 2022 Apr 26:114695. Epub 2022 Apr 26.

School of Computer Science and Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei, Nanjing, 210094, China. Electronic address:

Protein fold recognition is a critical step in protein structure and function prediction, and aims to ascertain the most likely fold type of the query protein. As a typical pattern recognition problem, designing a powerful feature extractor and metric function to extract relevant and representative fold-specific features from protein sequences is the key to improving protein fold recognition. In this study, we propose an effective sequence-based approach, called RattnetFold, to identify protein fold types. Read More

Anal Biochem 2022 Apr 26:114697. Epub 2022 Apr 26.

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, 14399-57131, Iran. Electronic address:

Breast cancer is the prevalent disease in women, and diagnosis of it in early stage and takes preventive measures is very critical. Recently, circulating microRNAs have emerged as promising early biomarkers of cancer. MiR-21 and miR-155 are two significant biomarkers that act as oncomir in breast cancer. Read More

Anal Biochem 2022 Jul 26;649:114694. Epub 2022 Apr 26.

School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang, 471000, China. Electronic address:

A novel ratiometric electrochemical aptasensor was constructed for the detection of carcinoembryonic antigen (CEA) based on a hairpin DNA (hpDNA) probe and exonuclease Ⅰ (Exo Ⅰ)-assisted target recycling amplification strategy. A thiolated methylene blue (MB)-labeled hpDNA as the internal control element was fixed on the surface of the gold nanoparticles (AuNPs)-modified gold electrode (AuE) through Au-S bonds. A ferrocene (Fc)-modified aptamer DNA (Fc-Apt) was partially hybridized with hpDNA to form a Fc-Apt/hpDNA duplex. Read More

Anal Biochem 2022 Jun 21;647:114692. Epub 2022 Apr 21.

Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, USA. Electronic address:

Despite the important role of membrane proteins in biological function and physiology, studying them remains challenging because of limited biomimetic systems for the protein to remain in its native membrane environment. Cryo electron microscopy (Cryo-EM) is emerging as a powerful tool for analyzing the structure of membrane proteins. However, Cryo-EM and other membrane protein analyses are better studied in a native lipid bilayer. Read More