Search our Database of Scientific Publications and Authors

I’m looking for a

    26519 results match your criteria Analytical Biochemistry [Journal]

    1 OF 531

    Direct analysis of PI(3,4,5)Pusing liquid chromatography electrospray ionization tandem mass spectrometry.
    Anal Biochem 2018 Feb 19. Epub 2018 Feb 19.
    Lilly Research Labs, Eli Lilly and Company, Indianapolis, IN, 46285, USA.
    Phosphatidylinositol (3,4,5) trisphosphate (PIP) is a biologically active membrane phospholipid that is essential for the growth and survival of all eukaryotic cells. We describe a new method that directly measures PIPand describe the HPLC separation and measurement of the positional isomers of phosphatidylinositol bisphosphate, PI(3,5)P, PI(3,4)Pand PI(4,5)P. Mass spectrometric analyses were performed online using ultra-high performance liquid chromatography (UHPLC)-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the negative multiple-reaction monitoring (MRM) modes. Read More

    Simultaneous detection of Mycoplasma pneumoniae IgG and IgM using dual-label time resolved fluoroimmunoassay.
    Anal Biochem 2018 Feb 17. Epub 2018 Feb 17.
    Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, 214023, China; Wuxi Children's Hospital, Wuxi, Jiangsu, 214023, China. Electronic address:
    Anti-Mycoplasma pneumoniae (MP) IgM and IgG are useful serological markers for detection of MP infection. In this study, a simultaneous quantification of MP IgM and IgG was performed by time-resolved fluoroimmunoassay (TRFIA). The europium-labeled anti-human IgM and samarium-labeled anti-human IgG were used as tracers, and MP IgM and IgG were recognized in serum samples. Read More

    Lectin-carbohydrate complex evaluation by chemiluminescence.
    Anal Biochem 2018 Feb 16. Epub 2018 Feb 16.
    Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária, 50030 Recife, Pernambuco, Brazil.
    In order to characterize the affinity between specific carbohydrate-binding proteins such as lectins, a model is proposed to study these interactions using a polysaccharide membrane to simulate such adsorption. Here, lectin-carbohydrate interactions were chemiluminescently investigated using lectins conjugated to acridinium ester (AE) and polysaccharides composed of their respective specific carbohydrates. The lectin-AE conjugates were incubated with discs (0. Read More

    Fluorescence-linked immunosorbent assay for detection of phenanthrene and its homolog.
    Anal Biochem 2018 Feb 16;547:45-51. Epub 2018 Feb 16.
    Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Beijing Hongwei Science & Technology Co., Ltd, Beijing 100000, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, PR China. Electronic address:
    A competitive fluorescence-linked immunosorbent assay (FLISA) was developed using rhodamine B isothiocyanate (RBITC) as the model fluorescent dye conjugate monoclonal antibody (McAb) for detection of Phe and its homolog (acenaphthene, fluorene, fluoranthene, pyrene and indeno [1,2,3-cd] pyrene) in water samples. The detection range of the assay for Phe was from 2.10 to 91. Read More

    Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles.
    Anal Biochem 2018 Feb 13;547:37-44. Epub 2018 Feb 13.
    School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address:
    Sensitive and accurate detection of tumor markers is critical to early diagnosis, point-of-care and portable medical supervision. Alpha fetoprotein (AFP) is an important clinical tumor marker for hepatocellular carcinoma (HCC), and the concentration of AFP in human serum is related to the stage of HCC. In this paper, a label-free electrochemical aptasensor for AFP detection was fabricated using AFP-aptamer as the recognition molecule and thionin/reduced graphene oxide/gold nanoparticles (TH/RGO/Au NPs) as the sensor platform. Read More

    Improved protein determination assays obtained after substitution of copper sulfate by copper oxide nanoparticles.
    Anal Biochem 2018 Feb 13;547:19-25. Epub 2018 Feb 13.
    Department of Botany, Faculty of Life Sciences, Maharshi Dayanand University, Rohtak 124001, India. Electronic address:
    Copper oxide nanoparticles (nano CuO) provide Cuions which can be easily harnessed for protein determination as an alternative to the use of copper sulfate (CuSO). In the present work, nano CuO of size <25 nm were substituted for CuSOin two of the well-known protein assays viz. Lowry method and bicinchoninic acid (BCA) method. Read More

    Analysis ofC labeling amino acids by capillary electrophoresis - High resolution mass spectrometry for fluxomic studies.
    Anal Biochem 2018 Feb 13;547:14-18. Epub 2018 Feb 13.
    Sorbonne Universités, Génie Enzymatique et Cellulaire, UMR CNRS 7025, Université de Technologie de Compiègne, 60205 Compiègne Cedex, France.
    In context of fluxomic studies,C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally,C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We developed a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Read More

    RPA using a multiplexed cartridge for low cost point of care diagnostics in the field.
    Anal Biochem 2018 Feb 12. Epub 2018 Feb 12.
    Centre for Electronic Systems Research, Electronic and Computer Engineering, CEDPS, Brunel University London, Kingston Lane, Uxbridge UB8 3PH, UK.
    A point of care device utilising Lab-on-a-Chip technologies that is applicable for biological pathogens was designed, fabricated and tested showing sample in to answer out capabilities. The purpose of the design was to develop a cartridge with the capability to perform nucleic acid extraction and purification from a sample using a chitosan membrane at an acidic pH. Waste was stored within the cartridge with the use of sodium polyacrylate to solidify or gelate the sample in a single chamber. Read More

    High-throughput screening identifies small molecules that bind to the RAS:SOS:RAS complex and perturb RAS signaling.
    Anal Biochem 2018 Feb 11. Epub 2018 Feb 11.
    Vanderbilt University School of Medicine, Department of Biochemistry, 2200 Pierce Ave., 802/804 RRB, Nashville, TN, 37232-0146, USA. Electronic address:
    K-RAS is mutated in approximately 30% of human cancers, resulting in increased RAS signaling and tumor growth. Thus, RAS is a highly validated therapeutic target, especially in tumors of the pancreas, lung and colon. Although directly targeting RAS has proven to be challenging, it may be possible to target other proteins involved in RAS signaling, such as the guanine nucleotide exchange factor Son of Sevenless (SOS). Read More

    Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay.
    Anal Biochem 2018 Feb 10;547:26-36. Epub 2018 Feb 10.
    Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China. Electronic address:
    We developed a colorimetric assay using yeast inorganic pyrophosphatase (IPP1) as a coupling enzyme to measure the activities of terpene synthases. IPP1 hydrolyzes pyrophosphate, the byproduct of terpene synthase catalyzed reactions, into orthophosphate, which can then be quantitated by reacting with molybdic acid to form a blue color compound. As a proof of concept, this method was used to quantitatively characterize three santalene synthases, SaSSy and SspiSSy involved in sandalwood oil biosynthesis, and a phylogenetically distant SanSyn from Clausena lansium. Read More

    Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7.
    Anal Biochem 2018 Feb 9. Epub 2018 Feb 9.
    Department of Oral Molecular Biology, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8588, Japan.
    Bacterial dipeptidyl-peptidase (DPP) 7 liberates a dipeptide with a preference for aliphatic and aromatic penultimate residues from the N-terminus. Although synthetic substrates are useful for activity measurements, those currently used are problematic, because they are more efficiently degraded by DPP5. We here aimed to develop a potent and specific substrate and found that the k/Kvalue for Phe-Met-methylcoumaryl-7-amide (MCA) (41. Read More

    Functional electrospun nanofibers-based electrochemiluminescence immunosensor for detection of the TSPusing RuAg/SiONPs as signal enhancers.
    Anal Biochem 2018 Feb 9. Epub 2018 Feb 9.
    Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
    A functional electrospun nanofibers-based electrochemiluminescence (ECL) immunosensor for detection of the tumor suppressor protein p53 (TSP) at trace level using core-shell nanocomposite as signal enhancers is fabricated. The gold nanoparticles (AuNPs) were decorated on the surface of the electrospun carbon-nanotubes (MWCNTs) doped chitosan (CTS) nanofibers (MWCNTs-CTS) by in-situ electrodeposition. The functional electrospun nanofibers (MWCNTs-CTS-AuNPs) was utilized as supporting scaffolds for TSPcapture antibody (C) immobilization firstly. Read More

    An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE.
    Anal Biochem 2018 Feb 9. Epub 2018 Feb 9.
    Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan; JST, PRESTO, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan; Molecular Chirality Research Center, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan. Electronic address:
    Membrane proteins, such as G-protein coupled receptors, control communication between cells and their environments and are indispensable for many cellular functions. Nevertheless, structural studies on membrane proteins lag behind those on water-soluble proteins, due to their low structural stability, making it difficult to obtain crystals for X-ray crystallography. Optimizing conditions to improve the stability of membrane proteins is essential for successful crystallization. Read More

    A convenient method of attaching fluorescent dyes on single-walled carbon nanotubes pre-wrapped with DNA molecules.
    Anal Biochem 2018 Feb 8;547:1-6. Epub 2018 Feb 8.
    Biophysics Section, Department of Physics, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 162-8601, Japan. Electronic address:
    We demonstrated the attachment of different kinds of dyes, Uranine, Rhodamime 800 (R800), and Indocyanine green (ICG), to single-walled carbon nanotubes pre-wrapped with single-stranded DNAs (ssDNA-SWCNTs). A new but simple method was employed, in which a dye solution was added to ssDNA-SWCNTs that had been prepared beforehand in the conventional way. Resulting conjugates of dyes, DNA, and SWCNTs were precisely evaluated by ultraviolet to near-infrared fluorescence/absorbance spectrometry and atomic force microscopy. Read More

    PD-1/PD-L1 inhibitor screening of caffeoylquinic acid compounds using surface plasmon resonance spectroscopy.
    Anal Biochem 2018 Feb 8;547:52-56. Epub 2018 Feb 8.
    Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address:
    Following the FDA approval of three monoclonal antibodies of PD-1/PD-L1, this pathway has become a promising target for cancer treatment. Currently small-molecule inhibitors have not been extensively investigated, and appropriate screening methods for such inhibitors are urgently required. In this study, surface plasmon resonance (SPR) technology was used to evaluate the affinity and competitive inhibition of nine caffeoylquinic acid compounds (CQAs) against PD-1/PD-L1. Read More

    Amplified detection of streptomycin using aptamer-conjugated palladium nanoparticles decorated on chitosan-carbon nanotube.
    Anal Biochem 2018 Feb 8;547:57-65. Epub 2018 Feb 8.
    Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran. Electronic address:
    A streptomycin-specific aptamer was used as a receptor molecule for ultrasensitive quantitation of streptomycin. The glassy carbon (GC) electrode was modified with palladium nanoparticles decorated on chitosan-carbon nanotube (PdNPs/CNT/Chi) and aminated aptamer against streptomycin. Modification of the sensing interface was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDS), wavelength-dispersive X-ray spectroscopy (WDX), cyclic voltammetry (CVs), and electrochemical impedance spectroscopy (EIS). Read More

    Development of a lateral flow recombinase polymerase assay for the diagnosis of Schistosoma mansoni infections.
    Anal Biochem 2018 Feb 7;546:65-71. Epub 2018 Feb 7.
    The Natural History Museum, Cromwell Road, London, UK. Electronic address:
    Infection with Schistosoma mansoni causes intestinal schistosomiasis, a major health problem across Africa. The accurate diagnosis of intestinal schistosomiasis is vital to inform surveillance/control programs. Diagnosis mainly relies on microscopic detection of eggs in faecal samples but many factors affect sensitivity. Read More

    Heterogeneous asymmetric recombinase polymerase amplification (haRPA) for rapid hygiene control of large-volume water samples.
    Anal Biochem 2018 Feb 9;546:58-64. Epub 2018 Feb 9.
    Institute of Hydrochemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Marchioninistrasse 17, 81377, München, Germany. Electronic address:
    Hygiene of drinking water is periodically controlled by cultivation and enumeration of indicator bacteria. Rapid and comprehensive measurements of emerging pathogens are of increasing interest to improve drinking water safety. In this study, the feasibility to detect bacteriophage PhiX174 as a potential indicator for virus contamination in large volumes of water is demonstrated. Read More

    A coupled fluorescence-based assay for the detection of protein arginine N-methyltransferase 6 (PRMT6) enzymatic activity.
    Anal Biochem 2018 Feb 2;547:7-13. Epub 2018 Feb 2.
    Institute of Pharmaceutical Chemistry, Goethe University, Max-von-Laue-Straße 9, D-60438, Frankfurt am Main, Germany. Electronic address:
    The protein arginine N-methyltransferase 6 (PRMT6) is overexpressed in a variety of different cancer types and plays a role in human immunodeficiency virus (HIV) infections. Furthermore, the PRMT6 activity might also influence the pathogenesis of neurodegenerative, inflammatory, and cardiovascular diseases, whereby it becomes an interesting target for drug development. Previously reported activity assays for PRMT6 activity are either expensive, time-consuming or use radioactive substrates. Read More

    Quack: A quality assurance tool for high throughput sequence data.
    Anal Biochem 2018 Jan 31. Epub 2018 Jan 31.
    Institute for Genomics, Biocomputing & Biotechnology, MS 39762, USA. Electronic address:
    The quality of data generated by high-throughput DNA sequencing tools must be rapidly assessed in order to determine how useful the data may be in making biological discoveries; higher quality data leads to more confident results and conclusions. Due to the ever-increasing size of data sets and the importance of rapid quality assessment, tools that analyze sequencing data should quickly produce easily interpretable graphics. Quack addresses these issues by generating information-dense visualizations from FASTQ files at a speed far surpassing other publicly available quality assurance tools in a manner independent of sequencing technology. Read More

    Identification and evaluation of Galleria mellonella peptides with antileishmanial activity.
    Anal Biochem 2018 Feb 1;546:35-42. Epub 2018 Feb 1.
    Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellin, Colombia. Electronic address:
    Leishmaniasis is a neglected disease, World Health Organization (WHO) declared it as high priority worldwide. Colombia is one of the 98 countries in which the disease caused more than 17.000 cases per year. Read More

    Platelet-derived microparticles analysis: Techniques, challenges and recommendations.
    Anal Biochem 2018 Feb 2;546:78-85. Epub 2018 Feb 2.
    Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP, 221005, India. Electronic address:
    Platelet-derived microparticles (PMP) are nano size fragments (100-1000 nm) released from platelets under various physiological and pathological conditions. PMP are the most abundant microparticles present in human blood. In recent past years PMP have caught attention of many clinicians as well as researchers for being associated with many diseases like cardio-vascular diseases, infections etc; and have emerged as potential biomarkers. Read More

    A strategy to analyse activity-based profiling of tyrosine kinase substrates in OCT-embedded lung cancer tissue.
    Anal Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Thoracic Surgery, University Hospital Zürich, Zürich, Switzerland.
    The use of optimal cutting temperature (OCT) medium has served to improve the long-term preservation of surgical tissue specimens. Unfortunately, the presence of polymers in OCT has been found to generate signal interference in proteomic-based techniques. Indeed the presence of OCT medium in tissue lysates precludes the analysis of activity based proteomic profiles obtained from lung adenocarcinoma (LuAdCa) resection specimens. Read More

    Kinetic studies of serine protease inhibitors in simple and rapid 'active barrier' model systems - Diffusion through an inhibitor barrier.
    Anal Biochem 2018 Jan 31;546:43-49. Epub 2018 Jan 31.
    Department of Chemistry - BMC Uppsala University, Box 576, SE-751 23 Uppsala, Sweden. Electronic address:
    A model based on gelatin for protease activity studies was designed. The model is also extended to study the efficiency of inhibitors in a separate protective layer covering the layer containing the target substrate. A good correlation between protease concentration and the size of erosion wells formed in a plain gelatin layer was observed. Read More

    Characterization of fatty acid amide hydrolase activity by a fluorescence-based assay.
    Anal Biochem 2018 Jan 31;546:50-57. Epub 2018 Jan 31.
    Institute II of Pharmacology, Center of Pharmacology, Medical Faculty, University of Cologne, Gleueler Str. 24, D-50931 Cologne, Germany. Electronic address:
    Fatty acid amide hydrolase (FAAH) is involved in many human diseases, particularly cancer, pain and inflammation as well as neurological, metabolic and cardiovascular disorders. Therefore, FAAH is an attractive target for the development of low-molecular-weight inhibitors as therapeutics, which requires robust assays that can be used for high-throughput screening (HTS) of compound libraries. Here, we report the development of a fluorometric assay based on FAAH's ability to effectively hydrolyze medium-chain fatty acid amides, introducing N-decanoyl-substituted 5-amino-2-methoxypyridine (D-MAP) as new amide substrate. Read More

    Recombinase polymerase amplification applied to plant virus detection and potential implications.
    Anal Biochem 2018 Jan 31;546:72-77. Epub 2018 Jan 31.
    North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Quincy, FL 32351, United States; Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, United States. Electronic address:
    Several isothermal techniques for the detection of plant pathogens have been developed with the advent of molecular techniques. Among them, Recombinase Polymerase Amplification (RPA) is becoming an important technique for the rapid, sensitive and cost-effective detection of plant viruses. The RPA technology has the advantage to be implemented in field-based scenarios because the method requires a minimal sample preparation, and is performed at constant low temperature (37-42 °C). Read More

    Microwave-assisted enzymatic hydrolysis of DNA for mass spectrometric analysis: A new strategy for accelerated hydrolysis.
    Anal Biochem 2018 Jan 31;546:28-34. Epub 2018 Jan 31.
    Department of Chemistry, Cleveland State University, 2121 Euclid Avenue, Cleveland, OH, USA. Electronic address:
    Study of DNA base composition, DNA adducts and modification in its primary structure is a subject of interest in different fields of scientific research. Various methods like immunochemistry, capillary electrophoretic separation, chromatographic separation coupled with mass spectrometric (LC-MS/MS) detection have been developed for DNA analysis. During the past decade LC-MS/MS has emerged as a more sensitive and selective technique and now frequently used for the analysis of DNA. Read More

    Reactivity-driven cleanup of 2-Aminobenzamide derivatized oligosaccharides.
    Anal Biochem 2018 Feb 1;546:23-27. Epub 2018 Feb 1.
    Analytical Research and Development, Pfizer, Inc., Andover, MA, USA.
    N-glycan profiling is commonly accomplished by the derivatization of the enzymatically released oligosaccharides with a fluorophore, thereby facilitating their analysis by hydrophilic-interaction liquid chromatography (HILIC). These fluorescent dyes are often present in large excess during derivatization reactions, and their removal is typically required to minimize chromatographic interference. Herein, we report a reactivity-driven 2-phase extraction protocol with the aldehyde reagent octanal, which demonstrated efficient 2-aminobenzamide cleanup as well as high derivatized N-glycan recovery. Read More

    A simple and effective method for detecting precipitated proteins in MALDI-TOF MS.
    Anal Biochem 2018 Jan 29;546:1-4. Epub 2018 Jan 29.
    Department of Pharmacology, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo, 173-8605, Japan. Electronic address:
    MALDI-TOF MS has developed rapidly into an essential analytical tool for the life sciences. Cinnamic acid derivatives are generally employed in routine molecular weight determinations of intact proteins using MALDI-TOF MS. However, a protein of interest may precipitate when mixed with matrix solution, perhaps preventing MS detection. Read More

    A liquid chromatography tandem mass spectroscopy approach for quantification of protein methylation stoichiometry.
    Anal Biochem 2018 Feb 2;545:72-77. Epub 2018 Feb 2.
    Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA. Electronic address:
    Post-translational modifications are biologically important and wide-spread modulators of protein function. Although methods for detecting the presence of specific modifications are becoming established, approaches for quantifying their mol modification/mol protein stoichiometry are less well developed. Here we introduce a ratiometric, label-free, targeted liquid chromatography tandem mass spectroscopy-based method for estimating Lys and Arg methylation stoichiometry on post-translationally modified proteins. Read More

    Voltammeric monitoring of linezolid, meropenem and theophylline in plasmas.
    Anal Biochem 2018 Jan 25;545:54-64. Epub 2018 Jan 25.
    National Organization for Drug Control and Research, Cairo, Egypt.
    Treatment of healthcare associated Pneumonia (HCAP) caused by Methicillin-resistant Staphylococcus aureus (MRSA) requires therapeutic protocols formed of linezolid (LIN) either alone or in combination with meropenem (MERO) and theophylline (THEO). The inter-individual pharmacokinetic variations require the development of reliable therapeutic drug monitoring (TDM) tools especially in immunocompromised patients. A sensitive square wave voltammetric sensor using multiwalled carbon nanotubes (MWCNTs) modified carbon paste electrode in Britton-Robinson buffer was developed and validated. Read More

    Zeolitic imidazolate framework-based biosensor for detection of HIV-1 DNA.
    Anal Biochem 2018 Jan 30;546:5-9. Epub 2018 Jan 30.
    Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), 1037 Luoyu Road, Wuhan 430074, China; Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China. Electronic address:
    ZIF-8(zinc-methylimidazolate framework-8), one of the zeolitic imidazolate frameworks (ZIFs), as quenching platforms for detection of HIV-1 DNA, which was identified to be effective for highly sensitive detection of HIV-1 DNA. The enhanced fluorescence signal has a relationship with the ssDNA concentration, the detection limit is as low as 1.2 nmol Lwith good selectivity. Read More

    Rapid detection of potyviruses from crude plant extracts.
    Anal Biochem 2018 Jan 31;546:17-22. Epub 2018 Jan 31.
    Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent, ME4 4TB, UK.
    Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp. Read More

    Duplex recombinase polymerase amplification assays incorporating competitive internal controls for bacterial meningitis detection.
    Anal Biochem 2018 Jan 30;546:10-16. Epub 2018 Jan 30.
    Molecular Diagnostics Research Group, School of Natural Sciences and National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
    Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technology that provides rapid and robust infectious disease pathogen detection, ideal for point-of-care (POC) diagnostics in disease-prevalent low-resource countries. We have developed and evaluated three duplex RPA assays incorporating competitive internal controls for the detection of leading bacterial meningitis pathogens. Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae singleplex RPA assays were initially developed and evaluated, demonstrating 100% specificity with limits of detection of 4. Read More

    Novel volumetric adsorptive microsampling technique for determination of perfluorinated compounds in blood.
    Anal Biochem 2018 Jan 25;545:49-53. Epub 2018 Jan 25.
    National Institute for Health and Welfare, Environmental Health Unit, Neulaniementie 4, FI-70210, Kuopio, Finland.
    Microsampling is an attractive option for significantly reducing the volume of blood taken for chemical analysis allowing for blood samples taken as a 'finger-prick' with a lancet. A novel, volumetric adsorptive microsampling (VAMS™) device, which reproducibly collects a small volume of 10 μL whole blood in a hematocrit-independent manner, is evaluated in a human biomonitoring setting, and has been utilized for analysis of several perfluoroalkyl acids (PFAA). The results show that the VAMS technique is applicable for PFAA analysis, method has good linearity, repeatability, accuracy and is sufficiently sensitive for samples from general populations. Read More

    Rapid flow-through enzyme immunoassay of progesterone in whole cows' milk.
    Anal Biochem 2018 Jan 19. Epub 2018 Jan 19.
    Department of Chemical Enzymology, Chemistry Faculty, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia; National University of Science and Technology "MISiS", 119991 Moscow, Russia.
    A rapid flow-through immunoassay using an enzyme (horseradish peroxidase) as a label for quantitative and semi-quantitative determination of progesterone in whole cows' milk was developed. The flow-through test device consisted of a porous nitrocellulose membrane coated with antibodies and an absorbent membrane. The substrate solution containing 3,3',5,5' -tetramethylbenzidine was used for colour visualization. Read More

    A novel in-cell ELISA method for screening of compounds inhibiting TRKA phosphorylation, using KM12 cell line harboring TRKA rearrangement.
    Anal Biochem 2018 Jan 27;545:78-83. Epub 2018 Jan 27.
    Department of In-vitro Biology, GVK Biosciences Private Limited, Campus MLR 1, Survey Nos. 125 (part) & 126, IDA Mallapur, Hyderabad, 500076, India. Electronic address:
    Tropomyosin-related kinase A (TRKA) fusion was originally detected in colorectal carcinoma that had resulted in expression of the oncogenic chimeric protein TPM3-TRKA. Lately, many more rearrangements in TRK family of kinases generating oncogenic fusion proteins have been identified. These genetic rearrangements usually result in fusion of cytoplasmic kinase domain of TRK to another gene of interest resulting in constitutive kinase activity. Read More

    Rapid detection of foodborne pathogen Listeria monocytogenes by strand exchange amplification.
    Anal Biochem 2018 Jan 20;545:38-42. Epub 2018 Jan 20.
    College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address:
    A strand exchange amplification (SEA) method to detect foodborne pathogen Listeria monocytogenes was developed. SEA is a novel nucleic acid amplification method that only requires one pair of primers. The specie-specific primers were designed by targeting the 16S rRNA gene and the amplification reaction was performed as short as 60 min at 61 °C. Read More

    Detection of protein targets with a single binding epitope using DNA-templated photo-crosslinking and strand displacement.
    Anal Biochem 2018 Jan 17;545:84-90. Epub 2018 Jan 17.
    Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China. Electronic address:
    DNA-based probes are powerful analytical tools for protein detection and analysis. Target-induced DNA assembly is a widely used strategy to transduce target-ligand binding to detectable signals. However, most of the existing methods based on DNA assembly require two or more binding sites on the target protein. Read More

    Multiplexed isothermal nucleic acid amplification.
    Anal Biochem 2018 Jan 17;545:20-30. Epub 2018 Jan 17.
    Interfibio Research Group, Department of Chemical Engineering, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain; ICREA, Passeig Lluís Companys 23, 08010 Barcelona, Spain. Electronic address:
    Multiplexed isothermal amplification and detection of nucleic acid sequences and biomarkers is of increasing importance in diverse areas including advanced diagnostics, food quality control and environmental monitoring. Whilst there are several very elegant isothermal amplification approaches, multiplexed amplification remains a challenge, requiring careful experimental design and optimisation, from judicious primer design in order to avoid the formation of primer dimers and non-specific amplification, applied temperature as well as the ratio and concentration of primers. In this review, we describe the various approaches that have been reported to date for multiplexed isothermal amplification, for both "one-pot" multiplexing as well as parallelised multiplexing using loop-mediated isothermal amplification, strand-displacement amplification, helicase-dependent amplification, rolling circle amplification, nucleic acid sequence-based amplification, with a particular focus on recombinase polymerase amplification. Read More

    Quantification of a recombinant antigen in an immuno-stimulatory whole yeast cell-based therapeutic vaccine.
    Anal Biochem 2018 Jan 12;545:65-71. Epub 2018 Jan 12.
    Biologics Analytical Operations, Pharmaceutical & Biologics Development, Gilead Sciences, 4010 Ocean Ranch Blvd, Oceanside, CA, 92056, United States. Electronic address:
    Therapeutic vaccines represent an emerging class of immune-modulatory treatments for cancer, infections, and chronic diseases. One such vaccine was designed as an immune stimulator of the T cell response against HBV antigens to eliminate HBV infected cells and offer a therapeutic avenue to treat patients suffering from chronic hepatitis B infection. Whole deactivated Saccharomyces cerevisiae cells expressing a recombinant fusion of HBV X, S and Core antigens elicit T cell responses in mice and activate human T cells linked with viral clearance. Read More

    Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.
    Anal Biochem 2018 Jan 12;545:4-12. Epub 2018 Jan 12.
    AI Biosciences, Inc., College Station, TX 77845, USA. Electronic address:
    Traditionally, the majority of nucleic acid amplification-based molecular diagnostic tests are done in centralized settings. In recent years, point-of-care tests have been developed for use in low-resource settings away from central laboratories. While most experts agree that point-of-care molecular tests are greatly needed, their availability as cost-effective and easy-to-operate tests remains an unmet goal. Read More

    Carboxylic group riched graphene oxide based disposable electrochemical immunosensor for cancer biomarker detection.
    Anal Biochem 2018 Jan 12;545:13-19. Epub 2018 Jan 12.
    Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore, 54000, Pakistan. Electronic address:
    In this work, we have developed for the first time a carboxylic group riched graphene oxide based disposable electrochemical immunosensor for cancer biomarker detection using methylene blue (MB). The developed immunosensor is highly sensitive for detection of biomarker Mucin1 (MUC1) in human serum samples. Development of this disposable electrochemical immunosensor was premeditated by applying specific monoclonal antibodies against MUC1. Read More

    A rapid fluorescent method for the real-time measurement of poly(ADP-ribose) polymerase 1 activity.
    Anal Biochem 2018 Jan 8. Epub 2018 Jan 8.
    Institute of Chemical Biology and Fundamental Medicine, Lavrentiev av. 8, 630090 Novosibirsk, Russia; Department of Natural Sciences, Novosibirsk State University, 2 Pirogov Street, 630090 Novosibirsk, Russia; Department of Physico-Chemical Biology and Biotechnology, Altai State University, 656049 Barnaul, Russia. Electronic address:
    Poly(ADP-ribose) polymerase 1 (PARP1) is a key enzyme that regulates important cellular processes, including DNA repair. PARP1 binds to a DNA damage site and synthesizes poly(ADP-ribose) chains (PARs), which serve as a signal of DNA damage for other DNA repair enzymes. PARP1 is a recognized target for the development of anti-cancer drugs. Read More

    Enhanced protein identification using graphite-modified MALDI plates for offline LC-MALDI-MS/MS bottom-up proteomics.
    Anal Biochem 2018 Jan 8;545:31-37. Epub 2018 Jan 8.
    Department of Chemistry, University of Wyoming, 1000 E. University Ave., Laramie, WY 82072, United States. Electronic address:
    The use of offline liquid chromatography-matrix assisted laser desorption/ionization (LC-MALDI) tandem mass spectrometry (MS/MS) for bottom-up proteomics offers advantages in terms of cost, ease of use, and the time-decoupled nature of the separation step and the mass analysis. A method was developed to improve the capabilities of LC-MALDI-MS/MS in terms of protein identification in a bottom-up proteomic workflow. Enhanced protein identification is achieved by an increase in the MALDI signal intensity of the precursor peptides brought about by coating the MALDI plate with a thin film of graphite powder. Read More

    Positive correlation between rat brain glutamate concentrations and mitochondrial 2-oxoglutarate dehydrogenase activity.
    Anal Biochem 2018 Jan 8. Epub 2018 Jan 8.
    Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskije gori 1, 119992, Moscow, Russia; A.N. Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University, Leninskije gori 1, 119992, Moscow, Russia. Electronic address:
    Glutamate is a key metabolite and major excitatory neurotransmitter, degraded through transformation to 2-oxoglutarate which is further catabolized by 2-oxoglutarate dehydrogenase complex (OGDHC). Both the glutamate excitotoxicity and impaired OGDHC activity are hallmarks of neurodegeneration. This work quantifies a relationship between the brain OGDHC activity and glutamate levels, assessing its diagnostic value to characterize (patho)physiology. Read More

    Drone inflight mixing of biochemical samples.
    Anal Biochem 2018 Jan 4;545:1-3. Epub 2018 Jan 4.
    Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia. Electronic address:
    Autonomous systems for sample transport to the laboratory for analysis can be improved in terms of timeliness, cost and error mitigation in the pre-analytical testing phase. Drones have been reported for outdoor sample transport but incorporating devices on them to attain homogenous mixing of reagents during flight to enhance sample processing timeliness is limited by payload issues. It is shown here that flipping maneuvers conducted with quadcopters are able to facilitate complete and gentle mixing. Read More

    A rapid method for identification and quantification of prostaglandins in cerebral tissues by UHPLC-ESI-MS/MS for the lipidomic in vivo studies.
    Anal Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Federal University of Santa Maria, Department of Pharmacology, Campus universitário, CEP 97105-900, Santa Maria, RS, Brazil.
    An analytical method utilizing liquid chromatography coupled to mass spectrometry with electrospray ionization has been developed for the identification of prostaglandins (PGs) in cerebral tissues. The five compounds identified (thromboxane B2, prostaglandin E2, prostaglandin D2, 6-keto-prostaglandin F1 alpha and prostaglandin F2 alpha) are cellular mediators of inflammation and are involved in a variety of physiological and pathological processes by acting on membrane receptors on the surfaces of target cells. The parameters of the electrospray ionization interface were optimized to obtain the highest possible sensitivity for all compounds studied. Read More

    Automated real-time detection of drug-resistant Mycobacterium tuberculosis on a lab-on-a-disc by Recombinase Polymerase Amplification.
    Anal Biochem 2018 Jan 2;544:98-107. Epub 2018 Jan 2.
    Biochemistry Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong. Electronic address:
    With the emergence of multi- and extensive-drug (MDR/XDR) resistant Mycobacterium tuberculosis (M. tb), tuberculosis (TB) persists as one of the world's leading causes of death. Recently, isothermal DNA amplification methods received much attention due to their ease of translation onto portable point-of-care (POC) devices for TB diagnosis. Read More

    A method for high transfection efficiency in THP-1 suspension cells without PMA treatment.
    Anal Biochem 2018 Jan 2;544:93-97. Epub 2018 Jan 2.
    Boston University, Henry M. Goldman School of Dental Medicine, Department of Periodontology, 100E.Newton Street, 02118, Boston, MA, United States.
    Adherent cells such as mouse RAW cells or human cancer U2OS cells are beneficial to DNA transfection, with 20%-60% transfection efficiency. However, this DNA transfection is rarely used on suspension cells due to its low transfection efficiency (≤5%). We recently found a new DNA transfection method to increase the efficiency up to 13. Read More

    1 OF 531