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    26631 results match your criteria Analytical Biochemistry [Journal]

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    Isothermal amplification using modified primers for rapid electrochemical analysis of coeliac disease associated DQB1*02 HLA allele.
    Anal Biochem 2018 Jun 16. Epub 2018 Jun 16.
    Interfibio Research Group, Department of Chemical Engineering, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain; ICREA, Passeig Lluís Companys 23, 08010, Barcelona, Spain. Electronic address:
    DNA biosensors are attractive tools for genetic analysis as there is an increasing need for rapid and low-cost DNA analysis, primarily driven by applications in personalized pharmacogenomics, clinical diagnostics, rapid pathogen detection, food traceability and forensics. A rapid electrochemical genosensor detection methodology exploiting a combination of modified primers for solution-phase isothermal amplification, followed by rapid detection via hybridization on gold electrodes is reported. Modified reverse primers, exploiting a C18 spacer between the primer-binding site and an engineered single stranded tail, are used in a recombinase polymerase amplification reaction to produce an amplicon with a central duplex flanked by two single stranded tails. Read More

    Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor.
    Anal Biochem 2018 Jun 16. Epub 2018 Jun 16.
    Department of Chemistry, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-City, Gyeonggi-Do, 461-701, South Korea. Electronic address:
    DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive oxygen species (ROS) created from the Fenton reaction. The adsorption of single-stranded DNA (ssDNA) onto the cationic surfactant-laden aqueous/LC interface interferes with the surfactant layer, inducing a planar orientation of the LCs. Read More

    An efficient method to evaluate experimental factor influence on in vitro binding of aptamers.
    Anal Biochem 2018 Jun 15. Epub 2018 Jun 15.
    Department of Chemistry, Capital Normal University, Xisanhuan North Road. 105, Beijing, 100048, China. Electronic address:
    Nucleic acid-based aptamers are promising alternative to antibodies, however, their selection process (SELEX) is challenging. A number of simulations and few experiments have been reported offering insights into experimental factors (EFs) that govern the effectiveness of the selection process. Though useful, these previous studied were either lack of experimental confirmation, or considered limited EFs. Read More

    Surveying GPCR solubilisation conditions using surface plasmon resonance.
    Anal Biochem 2018 Jun 14. Epub 2018 Jun 14.
    University of Dundee, Dow Street, Dundee, DD1 5EH, UK; Research Complex at Harwell, Rutherford Appleton Lab, Harwell, Oxford, OX11 0FA, UK.
    Biophysical screening techniques, such as surface plasmon resonance, enable detailed kinetic analysis of ligands binding to solubilised G-protein coupled receptors. The activity of a receptor solubilised out of the membrane is crucially dependent on the environment in which it is suspended. Finding the right conditions is challenging due to the number of variables to investigate in order to determine the optimum solubilisation buffer for any given receptor. Read More

    Simple colorimetric method to determine the in vitro antioxidant activity of different monoterpenes.
    Anal Biochem 2018 Jun 14. Epub 2018 Jun 14.
    Laboratorio de Nanobiomateriales, CINDEFI, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET (CCT La Plata), Calle 47 y 115, C.P. 1900 La Plata, Argentina. Electronic address:
    The development of simple, fast and reproducible techniques that provide information about the antioxidant activity (AA) of different compounds is essential to screen and discover new molecules with potential applications in the therapeutic, cosmetic, toxicological and food fields. Here, a novel and simple colorimetric method ("BCB assay") is proposed for measuring the AA of chemical compounds by protection of the reporter dye Brilliant Cresyl Blue (BCB) from loss of color due to oxidation by hypochlorite (a physiological oxidant). The decay in BCB blue color (λ = 634 nm) in the presence of hypochlorite occurred in only 5 min and was used to track the AA of different molecules. Read More

    Label-free detection of exonuclease III activity and its inhibition based on DNA hairpin probe.
    Anal Biochem 2018 Jun 17;555:55-58. Epub 2018 Jun 17.
    School of Life Sciences, Central South University, Changsha, 410013, China.
    In this paper, we have developed a label-free and rapid fluorescence assay for the detection of exonuclease III (exo III) activity via thioflavin T (ThT) as the G-quadruplex inducer. In this assay, a hairpin probe (HP) with a 5'-guanine-rich (G-rich) sequence is employed as the substrate for exo III. In the presence of exo III, HP can be digested at 3'-OH termini releasing 5'-G-rich sequence. Read More

    A molecularly-imprinted-electrochemical-sensor modified with nano-carbon-dots with high sensitivity and selectivity for rapid determination of glucose.
    Anal Biochem 2018 Jun 14;555:42-49. Epub 2018 Jun 14.
    School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:
    In this work, a novel molecularly imprinted electrochemical sensor (MIECS) based on a glassy carbon electrode (GCE) modified with carbon dots (CDs) and chitosan (CS) for the determination of glucose was proposed for the first time. The use of the environmental-friendly CDs and CS as electrode modifications improved the active area and electron-transport ability substantially, while 3-aminobenzeneboronic acid was used as a functional monomer and glucose as template for the fabrication of molecularly imprinted polymer (MIP) film to detect glucose via differential pulse voltammetry. Transmission electron microscope, Fourier transform infrared spectroscopy, energy dispersive x-ray spectrometry, cyclic voltammetry and electrochemical impedance spectroscopy (EIS) were applied to characterize the fabricated sensor. Read More

    Fabrication of glycerol biosensor based on co-immobilization of enzyme nanoparticles onto pencil graphite electrode.
    Anal Biochem 2018 Jun 13. Epub 2018 Jun 13.
    Department of Biochemistry, M.D.University, Rohtak, 124001, Haryana, India. Electronic address:
    Glycerol kinase (GK) and glycerol-3- phosphate oxidase (GPO) nanoparticles (NPs) were prepared, characterized and immobilized onto pencil graphite (PG) electrode to fabricate an improved amperometric glycerol biosensor (GKNPs/GPONPs/PGE). GKNPs/GPONPs/PGE worked in optimum conditions of pH 7.0, temperature 30 °C, at an applied potential of -0. Read More

    Amplified FRET based CA15-3 immunosensor using antibody functionalized luminescent carbon-dots and AuNPs-dendrimer aptamer as donor-acceptor.
    Anal Biochem 2018 Jun 13. Epub 2018 Jun 13.
    Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran.
    We proposed an amplified FRET immunosensing for detection of CA15-3 tumor marker by highly biospecific interactions between CA 15-3 antigen and the corresponding antibody and aptamer. In this sandwich type immunoassay, CA15-3 antibody-functionalized carbon dots and AuNPs labeled PAMAM-Dendrimer/aptamer were used as donor/acceptor, respectively. When CA 15-3 Ag was added to homogenous immunoassay, the strong complex interaction between CA15-3 Ab-CA15-3 Ag- aptamer caused in more coming closer carbon dot and AuNPs and more decreasing fluorescence signal. Read More

    A novel electrochemical DNA biosensor for Ebola virus detection.
    Anal Biochem 2018 Jun 13. Epub 2018 Jun 13.
    Advanced Energy & Sensor Lab, Department of Mechanical Engineering, The University of Akron, Akron, OH, 44325, United States. Electronic address:
    The aim of this study was to fabricate a novel electrochemical-based DNA-sensing device for Ebola virus DNA diagnostic by an enzyme-amplified detection, which improves the sensitivity and selectivity of the sensor. A thiolated DNA capture probe sequence was immobilized on the screen printed electrode surface and hybridized with biotinylated target strand DNA for the fabrication of Ebola DNA-sensing devices. Prior to the electrochemical detection of the enzymatic product by differential pulse voltammetry (DPV) method, the biotinylated hybrid was labelled with a streptavidin-alkaline phosphate conjugate on the surface of the working electrode. Read More

    Classifying the molecular functions of Rab GTPases in membrane trafficking using deep convolutional neural networks.
    Anal Biochem 2018 Jun 13;555:33-41. Epub 2018 Jun 13.
    Department of Computer Science and Engineering, Yuan Ze University, Chung-Li, 32003, Taiwan. Electronic address:
    Deep learning has been increasingly used to solve a number of problems with state-of-the-art performance in a wide variety of fields. In biology, deep learning can be applied to reduce feature extraction time and achieve high levels of performance. In our present work, we apply deep learning via two-dimensional convolutional neural networks and position-specific scoring matrices to classify Rab protein molecules, which are main regulators in membrane trafficking for transferring proteins and other macromolecules throughout the cell. Read More

    An impedimetric immunosensor for highly sensitive detection of IL-8 in human serum and saliva samples: A new surface modification method by 6-phosphonohexanoic acid for biosensing applications.
    Anal Biochem 2018 Jun 11;554:44-52. Epub 2018 Jun 11.
    Çanakkale Onsekiz Mart University, Faculty of Engineering, Bioengineering Department, Çanakkale, Turkey.
    In this study, we fabricated a sensitive and label-free impedimetric immunosensor based on 6-phosphonohexanoic acid (PHA) modified ITO electrode for detection of interleukin-8 (IL-8) in human serum and saliva. PHA was first employed to cancer biomarker sensing platform. Anti-IL-8 antibody was used as a biorecognition element and the detection principle of this immunosensor was based on monitoring specific interaction between anti-IL-8 antibody and IL-8 antigen. Read More

    Identification of adeno-associated virus capsid proteins using ZipChip CE/MS.
    Anal Biochem 2018 Jun 8;555:22-25. Epub 2018 Jun 8.
    Analytical Development, Biogen Inc, Cambridge, MA, USA.
    A simple and rapid identity test of adeno-associated virus (AAV) serotypes is important for supporting the AAV gene therapy development, as it relates to its efficacy and safety. The current mass spectrometry-based identity tests require extensive sample preparation steps, relatively large sample quantities and long analysis time. Herein, we describe a simple and novel microfluidic ZipChip CE/MS method used to characterize AAV capsid proteins. Read More

    Secondary structure assessment of formulated bevacizumab in the presence of SDS by deep ultraviolet resonance Raman (DUVRR) spectroscopy.
    Anal Biochem 2018 Jun 7;555:26-32. Epub 2018 Jun 7.
    Division of Pharmaceutical Analysis, Office of Testing and Research, Center for Drug Evaluation and Research, Food and Drug Administration, 645 S. Newstead Avenue, St Louis, MO, 63110, USA. Electronic address:
    A deep-ultraviolet resonance Raman (DUVRR) spectroscopic method has been used to study the secondary structural changes of a therapeutic monoclonal antibody (mAb), bevacizumab (Avastin™) under a chemical stress: the presence of sodium dodecyl sulfate (SDS). The results demonstrate that DUVRR spectroscopy can assay the higher order structure of the formulated protein in a sensitive and selective manner. The SDS-induced partially unfolding of the mAb was probed by DUVRR spectroscopy where the amide I, II and III spectral features showed conformational changes between beta-sheet, alpha-helix and random coil forms. Read More

    Coupling coumarin to gold nanoparticles by DNA chains for sensitive detection of DNase I.
    Anal Biochem 2018 Jun 6;555:50-54. Epub 2018 Jun 6.
    Key Laboratory of Analysis and Detection Technology for Food Safety (Ministry of Education and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350116, China. Electronic address:
    A kind of coumarin-modified gold nanoparticle by the bridge of dsDNA chains was designed and synthesized for sensitive detection of DNase I. The fluorescence of coumarin 343 at emission wavelengths of 491 nm excited at 440 nm was quenched by the gold nanoparticles due to the energy transfer process after the coumarin 343 was connected on the gold nanoparticles by DNA chains. When dsDNA chains were cut off by DNase I, the coumarin 343 molecules were released from gold nanoparticles and the fluorescence of coumarin 343 would be restored. Read More

    An impedimetric micro-immunosensing assay to detect Alzheimer's disease biomarker: Aβ40.
    Anal Biochem 2018 Jun 4;555:12-21. Epub 2018 Jun 4.
    Electrochemical Material and Sensors (EmaS) Group, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia; Faculty of Applied Sciences, Universiti Teknologi MARA, Tapah Campus, 35400 Tapah Road, Perak, Malaysia. Electronic address:
    A miniaturized biosensing platform, based on monoclonal amyloid-beta antibodies (mAβ) that were immobilized on a disc-shaped platinum/iridium (Pt/Ir) microelectrode surface coupled with an impedimetric signal transducer, was developed for the label-free and sensitive detection of amyloid-beta peptide fragment 1-40 (Aβ40); a reliable biomarker for early diagnosis of Alzheimer's disease (AD). A Pt/Ir microelectrode was electropolymerized with poly (ortho-phenylenediamine), a conducting free amine-containing aromatic polymer; followed by crosslinking with glutaraldehyde for subsequent coupling of mAβ on the microelectrode surface. This modification strategy efficiently improved the impedimetric detection performance of Aβ40 in terms of charge transfer resistance (∼400-fold difference) and normalized impedance magnitude percentage change (∼40% increase) compared with a passive adsorption-based immobilization method. Read More

    Analysis of the microbial cell-Ab binding in buffer solution by the piezoelectric resonator.
    Anal Biochem 2018 Jun 2;554:53-60. Epub 2018 Jun 2.
    Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Saratov Branch, Saratov, 410019, Russia.
    The possibility of the registration of the interaction of the cells Azospirillum lipoferum Sp59b with the specific antibodies directly in the conducting suspensions by using an acoustic sensor was shown. The main element of the sensor is a piezoelectric resonator with a lateral electric field. The analysis is based on a comparison of the resonator's electrical impedance before and after the specific biological interaction between the cells and antibodies. Read More

    Carbon nanotube-based aptasensor for sensitive electrochemical detection of whole-cell Salmonella.
    Anal Biochem 2018 Jun 2;554:34-43. Epub 2018 Jun 2.
    Institute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address:
    In this study, an amino-modified aptasensor using multi-walled carbon nanotubes (MWCNTs)-deposited ITO electrode was prepared and evaluated for the detection of pathogenic Salmonella bacteria. An amino-modified aptamer (ssDNA) which binds selectively to whole-cell Salmonella was immobilised on the COOH-rich MWCNTs to produce the ssDNA/MWCNT/ITO electrode. The morphology of the MWCNT before and after interaction with the aptamers were observed using scanning electron microscopy (SEM). Read More

    Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis.
    Anal Biochem 2018 Jun 2;555:9-11. Epub 2018 Jun 2.
    Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
    Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3-11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. Read More

    A novel approach for amine derivatization of MoS nanosheets and their application toward label-free immunosensor.
    Anal Biochem 2018 May 31;555:1-8. Epub 2018 May 31.
    CSIR-Central Scientific Instrument Organisation (CSIR-CSIO), Chandigarh, 160030, India; Academy of Scientific and Innovative Research (AcSIR-CSIO), Chandigarh, 160030, India. Electronic address:
    The application of molybdenum disulfide (MoS) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS nanosheets. Read More

    A competitive aptamer chemiluminescence assay for ochratoxin A using a single silica photonic crystal microsphere.
    Anal Biochem 2018 May 31;554:28-33. Epub 2018 May 31.
    Department of Food Science and Engineering, Nanjing Normal University, Nanjing, 210024, China.
    We designed a competitive aptamer chemiluminescence assay for ochratoxin A (OTA) on the surface of a single silica photonic crystal microsphere (SPCM) in cereal samples. The structural color of SPCMs is used to recognize and trace the microspheres during process of detection. Anti-aptamer was immobilized on the surface of SPCM. Read More

    Direct and quantitative electrophoretic detection of covalently closed DNA circles formed by in vitro ligation.
    Anal Biochem 2018 Jul 29;553:54-56. Epub 2018 May 29.
    Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan. Electronic address:
    The typical products of enzymatic circularization of DNA, using DNA ligase or recombinase, are covalently closed and mostly relaxed DNA circles. Because they are difficult to analyze on conventional gels, they are often converted to nicked circles prior to electrophoresis. Herein, we present a sensitive and quantitative procedure for directly analyzing ligated closed circle DNA on agarose gels without additional treatments. Read More

    A target-triggered biosensing platform for detection of HBV DNA based on DNA walker and CHA.
    Anal Biochem 2018 May 30;554:16-22. Epub 2018 May 30.
    Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address:
    Hepatitis B virus (HBV), one of the causative agents of viral hepatitis, may lead to chronic hepatitis, cirrhosis, and liver cancer. In this work, we designed a sensitive and modular biosensing platform for detecting HBV DNA based on a DNA walker that hangs on to surfaces and a catalyst-triggered catalyzed hairpin assembly (CHA). In the presence of HBV DNA, strand displacement reaction between target and double-stranded complex caused the release of walker strand to trigger the DNA walker. Read More

    Investigation of binding characteristics of immobilized toll-like receptor 3 with poly(I:C) for potential biosensor application.
    Anal Biochem 2018 May 26. Epub 2018 May 26.
    Department of Chemistry and Chemical Engineering, Royal Military College of Canada, P.O. Box 17000, Station Forces, Kingston, K7K 7B4, Ontario, Canada.
    Toll-like receptor 3 (TLR3), a pathogen recognition receptor of the innate immune response, recognizes and is activated by double-stranded RNA (dsRNA), which is indicative of viral exposure. A sensor design exercise was conducted, using surface plasmon resonance detection, through the examination of several immobilization approaches for TLR3 as a biorecognition element (BRE) onto a modified gold surface. To examine the TLR3-dsRNA interaction a synthetic analogue mimic, poly (I:C), was used. Read More

    Advanced tools for the analysis of protein phosphorylation in yeast mitochondria.
    Anal Biochem 2018 May 24;554:23-27. Epub 2018 May 24.
    Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria. Electronic address:
    The biochemical analysis of protein phosphorylation in mitochondria lags behind that of cytosolic signaling events. One reason is the poor stability of many phosphorylation sites during common isolation procedures for mitochondria. We present here an optimized, fast protocol for the purification of yeast mitochondria that greatly increases recovery of phosphorylated mitochondrial proteins. Read More

    Molecularly imprinted polymer based microtiter chemiluminescence array for determination of phenothiazines and benzodiazepines in pork.
    Anal Biochem 2018 May 25;554:9-15. Epub 2018 May 25.
    College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 071000, China. Electronic address:
    In this study, a molecularly imprinted polymer based chemiluminescence array capable of simultaneous determining phenothiazines and benzodiazepines was first reported. Two polymers were coated in different wells of the conventional 96-well microtiter plate as the recognition reagents, and the added analytes competed with a horseradish peroxidase-labeled bi-hapten conjugate to bind the recognition reagents. The light signal was induced by using a highly effective luminol-HO-IMP system. Read More

    Fluorescence alteration of MPA capped CdSe quantum dots by spontaneous biomarker protein adsorption.
    Anal Biochem 2018 May 23. Epub 2018 May 23.
    Department of Chemistry, Utah Valley University, Orem, UT 84058, USA. Electronic address:
    Quantum dots (QDs) have significant potentials in biomedical applications of bioimaging and biosensing. Spontaneous adsorption of proteins on QDs surface is a common phenomenon, which occurred to serum proteins in biological samples, and has been observed to enhance QDs fluorescence. In this study, fluorescence alteration of 3-mercaptopropionic acid (MPA) capped CdSe quantum dots by four individual biomarker proteins was investigated. Read More

    Au nanoparticles-ZnO composite nanotubes using natural silk fibroin fiber as template for electrochemical non-enzymatic sensing of hydrogen peroxide.
    Anal Biochem 2018 May 23;554:1-8. Epub 2018 May 23.
    Jiangsu Sinography Testing Co. Ltd., Nanjing 210032, PR China. Electronic address:
    A novel electrochemical sensor based on the composite of gold nanoparticles/zinc oxide nanotube (AuNPs/ZnO-NTs) was constructed and its application as hydrogen peroxide (HO) non-enzymatic sensor was investigated. ZnO-NTs were prepared by a biomineralization strategy in which silk fibroin fiber (SFF) was used as template, and thus the ZnO-NTs inherited the advantages of SFF such as mechanical stability, flexible biomimetic morphology and biocompatibility. The AuNPs/ZnO-NTs was further prepared by the electrostatic absorption of AuNPs onto the surface of ZnO-NTs, and found to be capable to catalyze the reduction of HO. Read More

    A simple electrochemical method to monitor an azo dye reaction with a liver protein.
    Anal Biochem 2018 Jul 23;553:46-53. Epub 2018 May 23.
    Institute of Chemistry, São Paulo State University (UNESP), 14801-970 Araraquara, SP, Brazil. Electronic address:
    Disperse Orange 37 (DO37) is an efficient azo dye for dyeing synthetic textile materials owing to its resistance to degradation that may also be harmful to humans as DO37 is not entirely eliminated in wastewater treatment. In this paper, we demonstrate that DO37 is bleached by reduced glutathione (GSH) in a reaction catalyzed by glutathione-s-transferase (GST), a phase II detoxification enzyme. The reaction included a nucleophilic attack involving sulfhydryl groups, confirmed using density functional theory (DFT) calculations. Read More

    An assay of optimal cytochrome c oxidase activity in fish gills.
    Anal Biochem 2018 Jul 19;553:38-45. Epub 2018 May 19.
    Department of Life Sciences, National Chung Hsing University, Taichung, 402, Taiwan; The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan. Electronic address:
    Cytochrome c oxidase (COX) catalyzes the terminal oxidation reaction in the electron transport chain (ETC) of aerobic respiratory systems. COX activity is an important indicator for the evaluation of energy production by aerobic respiration in various tissues. On the basis of the respiratory characteristics of muscle, we established an optimal method for the measurement of maximal COX activity. Read More

    Magnetic nanocomposite of chitosan-Schiff base grafted graphene oxide for lead analysis in whole blood.
    Anal Biochem 2018 Jul 18;553:28-37. Epub 2018 May 18.
    Analytical Chemistry Research Laboratory, Mobin Shimi Azma Company, Tehran, Iran.
    A new Schiff base grafted graphene oxide-magnetic chitosan was utilized as a novel sorbent for extraction and quantification of lead ion in blood samples via dispersive magnetic solid phase extraction. The prepared nanocomposite sorbent was characterized by SEM, TEM, FT-IR, XRD, VSM and EDX and the quantification analysis was performed by microsampling flame atomic absorption spectrometry. The important parameters on the extraction efficiency were thoroughly optimized by means of experimental design. Read More

    Electrochemical enzymatic fenitrothion sensor based on a tyrosinase/poly(2-hydroxybenzamide)-modified graphite electrode.
    Anal Biochem 2018 Jul 17;553:15-23. Epub 2018 May 17.
    Laboratório de Eletroquímica e Nanotecnologia Aplicada, Instituto de Ciência e Tecnologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rodovia MGT 367, Km 583, 5000, Alto da Jacuba, Diamantina, MG, 39100-000, Brazil. Electronic address:
    This paper reports the electrosynthesis and characterisation of a polymeric film derived from 2-hydroxybenzamide over a graphite electrode and its application as an enzymatic biosensor for the determination and quantification of the pesticide fenitrothion. The material was analysed by scanning electron microscopy and its electrochemical properties characterised by cyclic voltammetry and electrochemical impedance spectroscopy. The enzyme tyrosinase was immobilised over the modified electrode by the drop and dry technique. Read More

    Assay for abscisic acid 8'-hydroxylase activity of cloned plant cytochrome P450 oxidases in Saccharomyces cerevisiae.
    Anal Biochem 2018 Jul 16;553:24-27. Epub 2018 May 16.
    Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Liesel-Beckmann-Straße 1, 85354, Freising, Germany. Electronic address:
    The plant hormone abscisic acid (ABA) regulates many processes, including response to drought, seed dormancy and abscission of leaves and fruits. For maintenance of ABA homeostasis, catabolism of ABA by 8'-hydroxylation and subsequent cyclisation to phaseic acid (PA) is crucial. However, detection of ABA 8'-hydroxylation activity is tedious. Read More

    Development of DNAzyme-based PCR signal cascade amplification for visual detection of Listeria monocytogenes in food.
    Anal Biochem 2018 Jul 16;553:7-11. Epub 2018 May 16.
    School of Life Sciences, Shanghai University, Shanghai, 200444, PR China.
    Listeria monocytogenes is an important foodborne pathogen, and it can cause severe diseases. Rapid detection of L. monocytogenes is crucial to control this pathogen. Read More

    Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition.
    Anal Biochem 2018 Jul 25;553:12-14. Epub 2018 May 25.
    Department of Chemistry and Biochemistry, University of Montana, Missoula, MT, 59812, USA; Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, MT, 59812, USA. Electronic address:
    A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. Read More

    Isolation and characterization of a novel anti-salbutamol chicken scFv for human doping urinalysis.
    Anal Biochem 2018 May 15. Epub 2018 May 15.
    Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), 11800, Minden, Penang, Malaysia. Electronic address:
    Anti-salbutamol antibodies remain as important tools for the detection of salbutamol abuse in athletic doping. This study evaluated the feasibility and efficiency of the chicken (Gallus gallus domesticus) as an immunization host to generate anti-salbutamol scFv antibodies by phage display. A phage display antibody library was constructed from a single chicken immunized against salbutamol-KLH conjugate. Read More

    Coomassie does it (better): A Robin Hood approach to total protein quantification.
    Anal Biochem 2018 May 31. Epub 2018 May 31.
    Departments of Health Sciences and Biological Sciences, Faculties of Applied Health Sciences and Mathematics & Science, Brock University, St Catharines, Ontario, Canada. Electronic address:
    Quantitative comparative proteomics require accurate and reproducible assessments of total protein concentration. We report a straightforward, cost-effective adaptation of an established commercial method for total protein quantification (EZQ™), utilising non-proprietary materials and colloidal Coomassie Brilliant Blue (cCBB) staining to achieve greater reproducibility, equal sensitivity, and optimal linearity of signal within a practical concentration range for proteins in common solubilisation buffers (i.e. Read More

    Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film.
    Anal Biochem 2018 Jun 16;551:29-36. Epub 2018 May 16.
    Department of Chemistry, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia.
    In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR). The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX). The electroactivity of cobalt immobilized on f-MWCNTs-chitosan was assessed during the electro-oxidation of paracetamol. Read More

    Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices.
    Anal Biochem 2018 Jun 17;551:43-50. Epub 2018 May 17.
    Center for Neuroscience and Cell Biology, University of Coimbra, Portugal. Electronic address:
    The evaluation of mitochondrial function provides the basis for the study of brain bioenergetics. However, analysis of brain mitochondrial respiration has been hindered by the low yield associated with mitochondria isolation procedures. Furthermore, isolating mitochondria or cells results in loss of the inherent complexity of the central nervous system. Read More

    Adaptation of an amplicon-based human cancer next-generation sequencing panel assay for murine tumors.
    Anal Biochem 2018 Jun 10;551:26-28. Epub 2018 May 10.
    Kabara Cancer Research Institute, Gundersen Medical Foundation, 1300 Badger Street, La Crosse, WI 54601, USA; Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA. Electronic address:
    Unlike humans, inbred genetically engineered mice have minimal inter-individual variation and, consequently, offer substantially increased statistical power for robust definition of recurrent cooperating cancer mutations. While technically feasible, whole exome sequencing is expensive and extremely data-intensive. Somatic mutation analysis using panels of 25-75 genes now provides detailed insight into the biology of human tumors. Read More

    Metabolomics analysis of the potential anticancer mechanism of annonaceous acetogenins on a multidrug resistant mammary adenocarcinoma cell.
    Anal Biochem 2018 Jul 8;553:1-6. Epub 2018 May 8.
    Pharmaceutical Institute, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address:
    Although annonaceous acetogenins (ACGs) have been reported to have antitumor activity for over three decades, and many of the underlying mechanism of ACGs on cancer have been clarified, there are still outstanding issues. In particular, the changes of small metabolite in cancer cells, caused by ACGs intake, have been reported rarely. Recent research has showed that cellular metabolic profiling coupled with ultra-flow liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UFLC-Q-TOF-MS) and multivariable statistical analysis enables a good understanding of ACGs' effects on multidrug resistant human mammary adenocarcinoma (MCF-7/Adr) cells. Read More

    Long term stability of a HIV-1 neutralizing monoclonal antibody using isothermal calorimetry.
    Anal Biochem 2018 May 8. Epub 2018 May 8.
    Department of Biology, Johns Hopkins University, 3400 North Charles, Baltimore, MD 21218, United States. Electronic address:
    Different factors affect the long term stability of monoclonal antibodies, among them denaturation or partial denaturation that is often followed by aggregation. Isothermal calorimetry is capable of quantifying the kinetics of denaturation/aggregation of an antibody by measuring the heat that is released or absorbed by the process over a period of days or weeks, at temperatures below its denaturation temperature, T. The denaturation/aggregation kinetics of the anti-HIV monoclonal antibody VRC07-523LS was measured by isothermal calorimetry at different concentrations in four different formulation buffers. Read More

    Ultrasonic assisted magnetic dispersive solid phase microextraction for pre concentration of serotonin-norepinephrine reuptake inhibitor drugs.
    Anal Biochem 2018 Jun 8;551:7-18. Epub 2018 May 8.
    Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    A simple and sensitive ultrasonic assisted magnetic dispersive solid phase microextraction method (UAMDSPME) coupled with high performance liquid chromatography was developed to determine serotonin-norepinephrine reuptake inhibitor drugs including duloxetine (DUL), venlafaxine (VEN) and atomoxetine (ATO) in human urine, river water and well water samples. A novel and efficient SPME sorbent, magnetic p-Phenylenediamine functionalized reduced graphene oxide Quantum Dots@ Ni nanocomposites (MrGOQDs-PD@ Ni), was prepared and applied for extraction of the analytes. Several effective parameters on the extraction efficiency of the analytes were investigated and optimized with experimental design approach. Read More

    Continuous thrombin generation in whole blood: New applications for assessing activators and inhibitors of coagulation.
    Anal Biochem 2018 Jun 7;551:19-25. Epub 2018 May 7.
    University of Vermont, Department of Biochemistry, 360 South Park Drive, Colchester, VT 05446, USA. Electronic address:
    Hemostatic tests have been utilized to clarify the blood coagulation potential. The novel thrombin generation (TG) assay of this study provides explicit information and is the most physiologically-relevant hemostatic test ex vivo. We describe how this assay allows for TG under a number of relevant circumstances. Read More

    Quantification of Müllerian Inhibiting Substance/Anti-Müllerian Hormone polypeptide by isotope dilution mass spectrometry.
    Anal Biochem 2018 May 6. Epub 2018 May 6.
    National Institute for Biological Standards and Control, South Mimms, Potters Bar, EN6 3QG, UK.
    Measurement of serum concentrations of Müllerian inhibiting substance (MIS), also known as anti-Müllerian Hormone (AMH) by immunoassay is gaining clinical acceptance and widespread use for the diagnosis of ovarian conditions and for prediction of the response to ovarian stimulation protocols as part of assisted reproductive therapies. Provision of an International Standard to harmonize immunoassay methods is required. It is desirable for the content of a future International Standard to be assigned in mass units for consistency with the units reported by current methods. Read More

    Antibody-free detection of cellular neddylation dynamics of Cullin1.
    Anal Biochem 2018 May 5. Epub 2018 May 5.
    Takeda Pharmaceuticals International Co., 300 Massachusetts Avenue, Cambridge, MA, 02139, USA. Electronic address:
    Neddylation is a posttranslational modification that regulates protein stability, activity, and subcellular localization. Here, we describe a new tool for exploring the neddylation cycle of cullin1 (Cul1) directly in a cellular context. This assay utilizes the NanoLuc Binary Technology (NanoBiT) to monitor the covalent neddylation status of Cul1. Read More

    GC-MS determination of nitrous anhydrase activity of bovine and human carbonic anhydrase II and IV.
    Anal Biochem 2018 Jun 2;550:132-136. Epub 2018 May 2.
    Institute of Toxicology, Core Unit Proteomics, Hannover Medical School, Hannover, Germany. Electronic address:
    The most widely recognized activity of the large family of the metalloenzyme carbonic anhydrases (CAs) is the diffusion-controlled hydration of CO to HCO and one proton, and the less rapid dehydration of HCO to CO: CO + HO ⇆ HCO + H. CAs also catalyze the reaction of water with other electrophiles such as aromatic esters, sulfates and phosphates, thus contributing to lending to CAs esterase, sulfatase and phosphatase activity, respectively. Renal CAII and CAIV are involved in the reabsorption of nitrite, the autoxidation product of the signalling molecule nitric oxide (NO): 4 NO + O + 2 HO → 4 ONO + 4 H. Read More

    Feature-preserving noise reduction by using time-domain Gaussian-weighted multiple noise reduction filters for real-time bioluminescence measurement.
    Anal Biochem 2018 Jun 1;551:1-3. Epub 2018 May 1.
    Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan. Electronic address:
    This paper proposes a time-domain Gaussian-weighted noise reduction filter for bioluminescence measurement with low signal-to-noise ratio through photon counting. The filter was used for estimating the true fold-change signal from noisy gene expression data obtained through real-time dual-color luciferase assay. Furthermore, not only was the higher harmonics noise of the measurement system confirmed to reduce from the gene expression data but rapid and slow changes were also preserved in the estimated signal. Read More

    Electrocatalytic artificial carbonylation assay for observation of human serum albumin inter-individual properties.
    Anal Biochem 2018 Jun 30;550:137-143. Epub 2018 Apr 30.
    Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15, Olomouc, Czech Republic.
    Human serum albumin (HSA) is a multifunctional protein with ligand binding, transporting and buffering properties. Posttranslational modifications and ligand binding processes are closely related to albumin final functional status. In the last few decades, HSA has been characterized using a broad spectrum of methods, but quantitative data on the HSA's modifications among individuals have not been reported. Read More

    Bio-electrochemical microelectrode arrays for glutamate and electrophysiology detection in hippocampus of temporal lobe epileptic rats.
    Anal Biochem 2018 Jun 30;550:123-131. Epub 2018 Apr 30.
    State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 10090, China. Electronic address:
    Temporal Lobe Epilepsy (TLE) is a chronic neurological disorder, characterized by sudden, repeated and transient central nervous system dysfunction. For better understanding of TLE, bio-nanomodified microelectrode arrays (MEA) are designed, for the achievement of high-quality simultaneous detection of glutamate signals (Glu) and multi-channel electrophysiological signals including action potentials (spikes) and local field potentials (LFPs). The MEA was fabricated by Micro-Electro-Mechanical System fabrication technology and all recording sites were modified with platinum black nano-particles, the average impedance decreased by nearly 90 times. Read More

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