Direct analysis of PI(3,4,5)Pusing liquid chromatography electrospray ionization tandem mass spectrometry.
- Hai H Bui,
- Phillip E Sanders,
- Diane Bodenmiller,
- Ming Shang Kuo,
- Gregory P Donoho,
- Anthony S Fischl
Anal Biochem 2018 Feb 19. Epub 2018 Feb 19.
Lilly Research Labs, Eli Lilly and Company, Indianapolis, IN, 46285, USA.
Phosphatidylinositol (3,4,5) trisphosphate (PIP) is a biologically active membrane phospholipid that is essential for the growth and survival of all eukaryotic cells. We describe a new method that directly measures PIPand describe the HPLC separation and measurement of the positional isomers of phosphatidylinositol bisphosphate, PI(3,5)P, PI(3,4)Pand PI(4,5)P. Mass spectrometric analyses were performed online using ultra-high performance liquid chromatography (UHPLC)-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the negative multiple-reaction monitoring (MRM) modes. Read More