227,486 results match your criteria Analytical Biochemistry [Journal]


RNAi of Sterol Methyl Transferase1 Reveals its Direct Role in Diverting Intermediates Towards Withanolide/ Phytosterol Biosynthesis in Withania somnifera.

Plant Cell Physiol 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Aromatic Plants and Chiral Separation, Chemical Sciences Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), India.

The medicinal properties of Ashwagandha (Withania somnifera) are accredited to a group of compounds called as withanolides. 24-methylene cholesterol is the intermediate for sterol biosynthesis and a proposed precursor of withanolides biogenesis. But, conversion of 24-methylene cholesterol to withaferin A and other withanolides has not yet been biochemically dissected. Read More

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December 2018

Scattering characteristics of an exciton-plasmon nanohybrid made by coupling a monolayer graphene nanoflake to a carbon nanotube.

J Phys Condens Matter 2018 Dec 12. Epub 2018 Dec 12.

Advanced Computing and Simulation Laboratory, Monash University, Clayton,, Victoria 3800, Melbourne, Victoria, 3800, AUSTRALIA.

A hybrid nanostructure where a graphene nanoflake (GNF) is optically coupled to a carbon nanotube (CNT) could potentially possess enhanced sensing capabilities compared to the individual constituents whilst inheriting their high biocompatibility, favourable electrical, mechanical and spectroscopic properties. Therefore, in this paper, we investigate the scattering characteristics of an all-carbon exciton-plasmon nanohybrid which was made by coupling an elliptical GNF resonator to a semiconducting CNT gain element. We analytically model the nanohybrid as an open quantum system using cavity quantum electrodynamics. Read More

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December 2018

Log file based Monte Carlo calculations for proton pencil beam scanning therapy.

Phys Med Biol 2018 Dec 12. Epub 2018 Dec 12.

Center for Proton Therapy, Paul Scherrer Institute, WMSA/C12, Villigen - PSI, 5232, SWITZERLAND.

Patient specific quality assurance is crucial to guarantee safety in proton pencil beam scanning. In current clinical practice, this requires extensive, time consuming measurements. Additionally, these measurements do not consider the influence of density heterogeneities in the patient and are insensitive to delivery errors. Read More

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December 2018
1 Read

Self-assembled CpG oligodeoxynucleotides conjugated hollow gold nanospheres to enhance cancer-associated immunostimulation.

Colloids Surf B Biointerfaces 2018 Dec 3;175:248-255. Epub 2018 Dec 3.

Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China. Electronic address:

To realize efficiently cellular uptake and enhance the immunostimulation, thiolated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) were self-assembled on hollow gold nanospheres (HGNs) to form CpG-HGNs. The cellular uptake of CpG-HGNs in immune cells was studied in RAW 264.7 cells. Read More

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December 2018

Inferring symmetric and asymmetric interactions between animals and groups from positional data.

PLoS One 2018 12;13(12):e0208202. Epub 2018 Dec 12.

Department of Mathematics and Statistics, University of Western Australia, Nedlands, Perth, WA, Australia.

Interactions between domestic and wild species has become a global problem of growing interest. Global Position Systems (GPS) allow collection of vast records of time series of animal spatial movement, but there is need for developing analytical methods to efficiently use this information to unravel species interactions. This study assesses different methods to infer interactions and their symmetry between individual animals, social groups or species. Read More

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December 2018

[Anti-transglutaminase antibody in adults with celiac disease and their relation to the presence and duration of gluten-free diet].

Rev Gastroenterol Peru 2018 Jul-Sep;38(3):228-233

Departamento de Inmunología, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción. San Lorenzo, Paraguay.

IgA anti-transglutaminase 2 (tTG2) antibody is a relevant marker in celiac disease. The utility of IgA anti-tTG2 determination is well established for the diagnosis, however their use in the follow-up of patients with gluten free diet (GFD) it is not fully established.

Objective: To determine IgA anti-tTG2 antibody levels in adult Paraguayan celiac disease patients and its relation to the presence and duration of the GFD. Read More

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December 2018

Substantial differences in serum concentrations of psychoactive drugs measured in samples stored for two days or more on standard serum tubes versus serum tubes containing gel separators.

Ther Drug Monit 2018 Dec 11. Epub 2018 Dec 11.

Center for Psychopharmacology, Diakonhjemmet Hospital, Oslo, Norway.

Background: Drugs may potentially adsorb to blood collection tubes containing gel separators in the pre-analytical phase of therapeutic drug monitoring (TDM). The aim of this study was to compare measured concentrations of 28 psychoactive drugs and 13 metabolites in spiked serum samples stored on standard (plain) tubes vs. barrier gel tubes during a 2-6 days period at room temperature. Read More

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December 2018

Monitoring haemodynamic response to fluid-challenge in ICU: Comparison of pressure recording analytical method and oesophageal Doppler.

Eur J Anaesthesiol 2018 Dec 11. Epub 2018 Dec 11.

From the Department of Anaesthesia, Burn, and Critical Care, Saint-Louis Lariboisière University Hospital (RB, FD, MR, JS, AM, BGC), Department of Anaesthesia and Critical Care, European Hospital Georges-Pompidou, APHP (AN, DD, RP), Paris Diderot University, Sorbonne Paris Cité (AM, BGC), Inserm U942 (AM), Paris Descartes University, Sorbonne Paris Cité (RP), Inserm U1153 (RP) and Inserm U1160, Paris, France (BGC).

Background: The ability of the pressure recording analytical method (PRAM) in tracking change in cardiac output (ΔCO) after a fluid challenge in ICU needs to be evaluated with the most contemporary comparison methods recommended by experts.

Objective: Our objective was to report the trending ability of PRAM in tracking ΔCO after a fluid challenge in ICU and to compare this with oesophageal Doppler monitoring (ODM).

Design: Prospective, observational study. Read More

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December 2018

[Objective: The objective of this study is to determine the effects of mobile telephony on the improvement of post-exposure prophylaxis compliance at the Abidjan anti-rabies center.Methods: This is a descriptive and analytical cross-sectional study, which ran from September 2014 to May 2015. The data collection took place in two stages: first interview of all patients at the anti-rabies center at their first consultation, then a telephone interview three days after, only for those who had abandoned their prophylaxis.Results: The results indicated that after telephone calls, the dropout rate dropped from 59.3% to 44.8%. Discontinuations were less frequent in patients who received a veterinary surveillance notice (ORa = 0.23, 95% CI [0.11-0.48]), who had at least one veterinary certificate (ORa = 0.25 95% CI [0.15-0.43]) or more than two doses of vaccine (ORa = 0.23, 95% CI [0.12-0.42]) before telephone call. However, dropout rates were high in patients aged 20 to 29 years (ORa = 2.66, 95% CI [1.25-5.68]) and in patients with category III exposure (ORa = 2.19, 95% CI [1.12-54.3]).Conclusion].

Sante Publique 2018 Oct;30(4):545-554

Objective: The objective of this study is to determine the effects of mobile telephony on the improvement of post-exposure prophylaxis compliance at the Abidjan anti-rabies center.

Methods: This is a descriptive and analytical cross-sectional study, which ran from September 2014 to May 2015. The data collection took place in two stages: first interview of all patients at the anti-rabies center at their first consultation, then a telephone interview three days after, only for those who had abandoned their prophylaxis. Read More

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October 2018

Influence of socio-demographic characteristics in the self-care of people with heart failure.

Rev Bras Enferm 2018 ;71(suppl 6):2604-2611

Universidade Federal do Ceará. Fortaleza, Ceará, Brazil.

Objective: To determine the influence of socio-demographic characteristics in the self-care of people with heart failure (HF).

Method: Cross-sectional, analytical study, held in three private hospitals in Fortaleza, Ceará, Brasil, with 57 hospitalized patients. The data were collected through a demographic characterization form and a self-care assessment scale and were analyzed with inferential statistics, using mean comparison tests. Read More

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January 2018

A two-photon fluorescent probe for basal formaldehyde imaging in zebrafish and visualization of mitochondrial damage induced by FA stress.

Analyst 2018 Dec 12. Epub 2018 Dec 12.

Beijing Key Laboratory of Photo-electronic/Electro-photonic Conversion Materials, Key Laboratory of Cluster Science of Ministry of Education, School of Chemistry and Chemical Engineering, Analytical and Testing Centre, Beijing Institute of Technology, Beijing 100081, P. R. China.

The detection of mitochondrial formaldehyde (FA) is of great significance because FA is generated through a one-carbon formaldehyde cycle in mitochondria, and abnormal elevations in FA levels can damage mitochondria by decreasing the mitochondrial membrane potential and inhibiting mitochondrial respiration. Herein, a mitochondria-targetable two-photon probe (Mito-FA-FP) has been well demonstrated. Mito-FA-FP is conjugated with hydrazine as the FA-reactive site and a pyridine derivate as the mitochondria-targetable moiety. Read More

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December 2018

Body mass index and body fat percentage in assessing obesity: An analytical study among the adolescents of Dibrugarh, Assam.

Indian J Public Health 2018 Oct-Dec;62(4):277-281

Professor, Department of Community Medicine, Gauhati Medical College, Guwahati, Assam, India.

Background: Body mass index (BMI) is one of the most commonly used indices to measure the weight status of an individual. However, it takes only height and weight of individual into account. The relative body composition can be calculated regardless of height and weight by body fat percentage (BF%). Read More

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December 2018

Detection of urinary trace elements and pattern recognition analysis in patients with renal cell carcinoma by inductively coupled plasma mass spectrometry.

J Cancer Res Ther 2018 Dec;14(Supplement):S1152-S1157

Department of Chemistry, Key Laboratoryof Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Objective: This study aims to observe the changes and diagnostic values of urinary trace elements in patients with renal cell carcinoma (RCC).

Methods: A total of 28 RCC patients that had been performed radical surgery for more than 3 years were included into this case-control study; meanwhile, thirty healthy volunteers without kidney diseases were set as the control group over the same period. The levels of various urinary trace elements in both groups were measured, and the results were performed the significance analysis and pattern recognition analysis using with partial least square discriminant analysis and Fisher analysis. Read More

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December 2018

Novel multiplex real-time quantitative PCR detecting system approach for direct detection of Candida auris and its relatives in spiked serum samples.

Future Microbiol 2018 Dec 12. Epub 2018 Dec 12.

Westerdijk Fungal Biodiversity Institute, Utrecht 3584, The Netherlands.

The multidrug-resistant opportunistic yeast species of Candida auris, Candida haemulonii, Candida duobushaemulonii and Candida pseudohaemulonii continue to endanger the healthcare settings around the globe. Due to the lack of a specific qPCR assay for detection of these species from clinical samples, we developed a multiplex qPCR assay. Analytical specificity and sensitivity showed 100% specificity and the sensitivity of up to ten genomes of target species with a high value of reproducibility (R >0. Read More

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December 2018

Voltammetric analysis of dantrolene and its active metabolite with indomethacin in rat plasma.

Bioanalysis 2018 Dec 12. Epub 2018 Dec 12.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, El-Messalah, Alexandria 21521, Egypt.

Aim: Differential pulse polarography was used for the concurrent analysis of the coadministered dantrolene (DAN) and indomethacin (IND) in plasma.

Materials & Methods: DAN and IND, Hanging mercury drop electrode and Britton-Robinson buffer at pH 5 were used. In plasma, cathodic reduction of DAN nitro group and its active metabolite at -0. Read More

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December 2018

Miniaturized sample preparation methods for saliva analysis.

Bioanalysis 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemistry, Faculty of Science, Qom University, Qom, Iran.

Saliva, as the first body fluid encountering with the exogenous materials, has good correlation with blood and plays an important role in bioanalysis. However, saliva has not been studied as much as the other biological fluids mainly due to restricted access to its large volumes. In recent years, there is a growing interest for saliva analysis owing to the emergence of miniaturized sample preparation methods. Read More

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December 2018

Autoantibodies as Diagnostic Tools.

Authors:
Gunnar Houen

Methods Mol Biol 2019 ;1901:1-11

Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark.

Autoimmune diseases are very diverse and include many common diseases of unknown etiology.Diagnosis can be challenging but can be facilitated by the identification of characteristic autoantibodies (AuAbs), which are present in varying frequencies. Identification of such AuAbs requires a range of different techniques, depending on the autoantigens in question. Read More

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January 2019

Mapping and Quantification of tRNA 2'-O-Methylation by RiboMethSeq.

Methods Mol Biol 2019 ;1870:273-295

Next-Generation Sequencing Core Facility, UMS2008 IBSlor, CNRS-UL-INSERM, BioPole Lorraine University, Nancy, France.

Current development of epitranscriptomics field requires efficient experimental protocols for precise mapping and quantification of various modified nucleotides in RNA. Despite important advances in the field during the last 10 years, this task is still extremely laborious and time-consuming, even when high-throughput analytical approaches are employed. Moreover, only a very limited subset of RNA modifications can be detected and only rarely be quantified by these powerful techniques. Read More

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January 2019

A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning.

Methods Mol Biol 2019 ;1904:377-400

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.

Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate. The advantage of using a magnetic particle processor system is the ability to carry out phage display panning against multiple target antigens simultaneously in parallel. Read More

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January 2019

Purification of Human Monoclonal Antibodies and Their Fragments.

Methods Mol Biol 2019 ;1904:163-188

ETH Zurich, Institute for Chemical and Bioengineering, Zurich, Switzerland.

This chapter summarizes the most common chromatographic mAb and mAb fragment purification methods, starting by elucidating the relevant properties of the compounds and introducing the various chromatography modes that are available and useful for this application. A focus is put on the capture step affinity and ion-exchange chromatography. Aspects of scalability play an important role in judging the suitability of the methods. Read More

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January 2019

An Overview of DNA Analytical Methods.

Methods Mol Biol 2019 ;1897:385-402

Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

The development of rapid parallel sequencing in the last 20 years has begun a revolution in the field of genetics that is changing nearly all disciplines within biology and medicine. Genomic sequencing has become crucial to the diagnosis and clinical management of patients with constitutional diseases and cancer and has quickly become an integral part of the new era of personalized and precision medicine. The precision medicine initiative, released by the NIH in 2015, has catapulted genomic technologies to the forefront of the practice of medicine and biomedical research. Read More

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January 2019

Nanostructured tungsten sulfides: insights into precursor decomposition and the microstructure using X-ray scattering methods.

Dalton Trans 2018 Dec 12. Epub 2018 Dec 12.

Institute of Inorganic Chemistry, Kiel University, Max-Eyth Straβe 2, D-24118 Kiel, Germany.

Herein we present an in-depth study of precursor derived tungsten sulfides, with a focus on their micro- and local structures. We prepared a new tetrathiotungstate based precursor (N2H5)2WS4 and unveiled the details of its unique decomposition mechanism by a combination of in situ and ex situ analytical techniques. Upon heating the precursor, a new compound with composition (NH4)(N2H5)WS4 is formed by the decomposition of one hydrazinium molecule. Read More

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December 2018

Development of an analytical prepCheck-supported approach to evaluate tutor-based assessments of dental students' practical skills.

Int J Comput Dent 2018 ;21(4):313-322

The aim of the present study was to develop an analytical computer-supported assessment concept for the objective evaluation of students' practical skills, and to investigate the assessor-dependent differences by setting tolerance limits applied during the 'glance and grade' evaluation. A sample of 54 conventionally supervised dental undergraduates were given training in the preparation of a macroretentive Class II cavity. The course participants then took a mandatory practical examination. Read More

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January 2018

Evaluation of the Starch Quantification Methods of and Using Factorial Experiments.

Int J Food Sci 2018 13;2018:5901930. Epub 2018 Nov 13.

Universidad de Cartagena, Cartagena de Indias, Colombia.

Background: Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material.

Method: Three methods were used in triplicate: the first followed the official technique of the Association of Official Analytical Chemists (AOAC), to perform acid hydrolysis and quantification of starch by Titulation; the second method involved the colorful reaction with iodine using the UV equipment to measure the absorbance and calculate the percentage of starch; as a third method the FTIR was used, through which the concentration of the starch was calculated by the area under the curve obtained from the spectrum. Read More

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November 2018

Solid Contact Potentiometric Sensors Based on a New Class of Ionic Liquids on Thiacalixarene Platform.

Front Chem 2018 27;6:594. Epub 2018 Nov 27.

Department of Organic Chemistry, A. M. Butlerov' Chemistry Institute, Kazan Federal University, Kazan, Russia.

New solid-contact potentiometric sensors have been developed for hydrogen phosphate recognition on the basis of ionic liquids containing tetrasubstituted derivatives of thiacalix[4]arene in and conformations with trimethyl- and triethylammonium fragments at the lower rim substituents. The recognition of selected anions including carbonate, hydrogen phosphate, perchlorate, oxalate, picrate, and EDTA was conducted using electrochemical impedance spectroscopy with ferricyanide redox probe. For the potentiometric sensor assembling, the ionic liquids were stabilized by multiwalled carbon nanotubes and carbon black deposited on the glassy carbon electrode. Read More

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November 2018

Simultaneous Determination of Cr, As, Se, and Other Trace Metal Elements in Seawater by ICP-MS with Hybrid Simultaneous Preconcentration Combining Iron Hydroxide Coprecipitation and Solid Phase Extraction Using Chelating Resin.

Int J Anal Chem 2018 13;2018:9457095. Epub 2018 Nov 13.

Department of Environmental Science, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.

In the present study, ICP-MS with a new hybrid simultaneous preconcentration combining solid phase extraction using chelating resin and iron hydroxide coprecipitation in one batch at a single pH adjustment (pH 6.0) were developed for multielement determination of trace metal ions in seawater. In multielement determination, the present method makes it possible to determine Cr(III), As(V), Se (IV), and other 14 trace metal elements (Ti, V, Co, Ni, Cu, Zn, Zr, Ge, Cd, Sb, Sn, W, Pb, and U) in seawater. Read More

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November 2018

Quantitative Determination of 18--Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method.

Int J Anal Chem 2018 13;2018:5673186. Epub 2018 Nov 13.

UOC Odontoiatria Generale e Ortodonzia, Dip Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, Italy.

A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18--glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 m) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CHOH and 20% of CHCN: tetrahydrofuran: water (10:80:10, v/v/v). Read More

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November 2018
2 Reads

Quantifying Inorganic Nitrogen Assimilation by Using Bulk and Single-Cell Mass Spectrometry: A Comparative Study.

Front Microbiol 2018 27;9:2847. Epub 2018 Nov 27.

Climate Change Cluster, University of Technology Sydney, Sydney, NSW, Australia.

Microorganisms drive most of the major biogeochemical cycles in the ocean, but the rates at which individual species assimilate and transform key elements is generally poorly quantified. One of these important elements is nitrogen, with its availability limiting primary production across a large proportion of the ocean. Nitrogen uptake by marine microbes is typically quantified using bulk-scale approaches, such as Elemental Analyzer-Isotope Ratio Mass Spectrometry (EA-IRMS), which averages uptake over entire communities, masking microbial heterogeneity. Read More

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November 2018

A methodological review of national and transnational pharmaceutical budget impact analysis guidelines for new drug submissions.

Clinicoecon Outcomes Res 2018 26;10:821-854. Epub 2018 Nov 26.

Department of Health Research Methods, Evidence and Impact (HEI), McMaster University, Hamilton, ON, Canada,

Introduction: Budget impact analysis (BIA) in health care, sometimes referred to as resource impact, is the financial change in the use of health resources associated with adding a new drug to a formulary or the adoption of a new health technology. Several national and transnational organizations worldwide have updated their BIA guidelines in the past 4 years. The aim of the present review was to provide a comprehensive list of the key recommendations of BIA guidelines from different countries that may be of interest for those who wish to build or to update BIA guidelines. Read More

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November 2018

Structural and functional comparability study of anti-CD20 monoclonal antibody with reference product.

Biologics 2018 23;12:159-170. Epub 2018 Nov 23.

Sun Pharmaceutical Industries Limited, Tandalja, Vadodara 390 012, India,

Background: Cell surface protein, CD20, is extensively expressed on the surface of B cells. Antibodies targeting CD20 protein are being used to treat B-cell malignancies and B-cell mediated autoimmune diseases. Considering the cost of therapy with innovator monoclonal antibodies for these diseases, development of biosimilar products for the treatment of such diseases provides affordable solution to rising healthcare costs. Read More

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November 2018

Post-GWAS in prostate cancer: from genetic association to biological contribution.

Nat Rev Cancer 2018 Dec 11. Epub 2018 Dec 11.

Cancer Program, School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.

Genome-wide association studies (GWAS) have been successful in deciphering the genetic component of predisposition to many human complex diseases including prostate cancer. Germline variants identified by GWAS progressively unravelled the substantial knowledge gap concerning prostate cancer heritability. With the beginning of the post-GWAS era, more and more studies reveal that, in addition to their value as risk markers, germline variants can exert active roles in prostate oncogenesis. Read More

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December 2018
37.400 Impact Factor

A quarter of a century of SERF: The progress of an NMR pulse sequence and its application.

Authors:
Stefan Berger

Prog Nucl Magn Reson Spectrosc 2018 Oct 28;108:74-114. Epub 2018 Nov 28.

Institute of Analytical Chemistry, University Leipzig, Johannisallee 29, D-04103 Leipzig, Germany. Electronic address:

SERF, an NMR pulse sequence for selectively measuring a spin coupling constant without interference from other couplings, was published by the current author almost 25 years ago in 1995. Since then, about 35 modifications and extensions of the original have been published by other groups and applied to many chemical problems. This review discusses these modifications and provides pertinent examples. Read More

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October 2018

Criteria for specific measurement of plasminogen (enzymatic; procedure) in human plasma.

EJIFCC 2000 Sep 1;12(3):83-91. Epub 2000 Sep 1.

Chromogenix AB, Mölndal, Sweden.

There is a lack of well-established criteria for the specific measurement of fibrinolytic variables. On behalf of the SSC the Subcommittee on Fibrinolysis started a process to develop such criteria. This report describes the criteria for the measurement of plasminogen (enzymatic; procedure) in human plasma. Read More

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September 2000

Study of Sensitivity and Resolution for Full Ring PET Prototypes based on Continuous Crystals and analytical modeling of the light distribution.

Phys Med Biol 2018 Dec 11. Epub 2018 Dec 11.

Instituto de Fisica Corpuscular, Universidad de Valencia, Edificio Institutos de Paterna, Apartado de Correos 22085, E-46071 Valencia, Valencia, SPAIN.

Sensitivity and spatial resolution are the main parameters to maximize in the performance of a PET scanner. For this purpose, detectors consisting of a combination of continuous crystals optically coupled to segmented photodetectors have been employed. With the use of continuous crystals the sensitivity is increased with respect to the pixelated crystals. Read More

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December 2018
1 Read

Development of an in house ELISA for human intact osteocalcin and its utility in diagnosis and management of osteoporosis.

Clin Chim Acta 2018 Dec 8. Epub 2018 Dec 8.

Department of Molecular Immunodiagnostics, ICMR-National Institute for Research In Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai, India. Electronic address:

Background: Serum Osteocalcin (OC) is a biomarker for evaluating bone turnover in humans. Commercial kits of OC in India are imported, hence expensive prohibiting their use in routine screening of osteoporosis. The present study describes the development, validation of human OC ELISA and establishes cut off values for its use in screening and management of women at risk for osteoporosis. Read More

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December 2018
1 Read

Spectrofluorimetric determination of eptifibatide in human plasma and dosage form.

Luminescence 2018 Dec 11. Epub 2018 Dec 11.

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El- Aini Street, Cairo, Egypt.

A spectrofluorimetric method for the determination of eptifibatide is presented based on its native fluorescence. The type of solvent and the wavelength of maximum excitation and emission were carefully selected to optimize the experimental conditions. Under the specified experimental conditions, the linearities obtained between the emission intensity and the corresponding concentrations of eptifibatide were in the range 0. Read More

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December 2018
1 Read

Estimating marginal proportions and intraclass correlations with clustered binary data.

Biom J 2018 Dec 11. Epub 2018 Dec 11.

Biostatistics, Department of Basic Clinical Practice, University of Barcelona, Barcelona, Spain.

A logistic regression with random effects model is commonly applied to analyze clustered binary data, and every cluster is assumed to have a different proportion of success. However, it could be of interest to obtain the proportion of success over clusters (i.e. Read More

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December 2018

Structural analysis of the inhibitory effects of polyphenols, (+)-hopeaphenol and (-)-isohopeaphenol, on human SIRT1.

Biofactors 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Analytical Neurobiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya, Japan.

Human sirtuin 1 (hSIRT1) is a NAD -dependent deacetylase that regulates several cellular processes. Unlike resveratrol, natural polymeric phenolic compounds isolated from Vitaceae are mostly hSIRT1 inhibitors. The resveratrol tetramer, (+)-hopeaphenol ((+)-HP), and its geometric isomer, (-)-isohopeaphenol ((-)-iHP), were tested for inhibitory effects on purified hSIRT1 using a fluorescent derivative of peptide substrate p53-AMC (Fluor de Lys) and a cofactor NAD . Read More

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December 2018

Evaluation of next-generation sequencing-based clonality analysis of T-cell receptor gamma gene rearrangements based on a new interpretation algorithm.

Int J Lab Hematol 2018 Dec 8. Epub 2018 Dec 8.

Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium.

Introduction: T-cell receptor gene (TRG) rearrangement profiling is an essential component of the workup at diagnosis of T-cell malignancies. TRG amplification by polymerase chain reaction (PCR) and analysis by capillary electrophoresis (PCR-CE) is mostly widely used but is hampered by a subjective interpretation of its results and possible false-positive interpretation of clonality. Several studies evaluated the advantage of TRG rearrangement analysis by Next Generation Sequencing (TRG-NGS), however few have proposed an adequate data interpretation algorithm. Read More

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December 2018

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification.

Lett Appl Microbiol 2018 Dec 7. Epub 2018 Dec 7.

School of Chemical Engineering and Science, Swinburne University of Technology, Sarawak Campus, Jalan Simpang Tiga, 93350, Kuching, Sarawak, Malaysia.

Biocalcification through the use of ureolytic bacteria and biochemical activities has evolved in recent decades into a fervent resourceful effective technology suitable for soil stabilization, crack repair and bioremediation. Extensive studies have been carried out numerous ureolytic bacterial species isolated from soils and sewage samples. However, very limited attention has been given to limestone caves with natural calcite formations, as a possible source for isolation of ureolytic bacteria. Read More

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December 2018

Dielectric and Resistive Heating of Polymeric Media: Toward Remote Thermal Activation of Stimuli-Responsive Soft Materials.

Macromol Rapid Commun 2018 Dec 11:e1800669. Epub 2018 Dec 11.

Department of Chemical & Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA.

Stimuli-responsive soft materials are becoming increasingly important in a wide range of contemporary technologies, and methods by which to promote thermal stimulation remotely are of considerable interest for controllable device deployment, particularly in inaccessible environments such as outer space. Until now, remote thermal stimulation of responsive polymers has relied extensively on the use of nanocomposites wherein embedded nanoparticles/structures are selectively targeted for heating purposes. In this study, an alternative remote-heating mechanism demonstrates that the dielectric and resistive thermal losses introduced upon application of an alternating current generate sufficient heat to raise the temperature of a neat polyimide by over 70 °C within ≈10 s. Read More

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December 2018

The convenient use of fluorescamine for spectrofluorimetric determination of midodrine hydrochloride in pure form and its tablets formulation: Application to content uniformity testing.

Luminescence 2018 Dec 10. Epub 2018 Dec 10.

Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.

A novel sensitive and simple spectrofluorimetric method was developed then validated for determination of midodrine in both its authentic pure form and its tablets. This method is based on the reaction between midodrine's aliphatic primary amine moiety with fluorescamine reagent, using borate buffer at pH 7.8 and yielding a highly fluorescent product whose fluorescence intensity was measured at 462 nm after excitation at 388 nm. Read More

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December 2018

Critical Considerations on the Clinical Translation of Upconversion Nanoparticles (UCNPs): Recommendations from the European Upconversion Network (COST Action CM1403).

Adv Healthc Mater 2018 Dec 10:e1801233. Epub 2018 Dec 10.

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany.

The unique photoluminescent properties of upconversion nanoparticles (UCNPs) have attracted worldwide research interest and inspired many bioanalytical applications. The anti-Stokes emission with long luminescence lifetimes, narrow and multiple absorption and emission bands, and excellent photostability enable background-free and multiplexed detection in deep tissues. So far, however, in vitro and in vivo applications of UCNPs are restricted to the laboratory use due to safety concerns. Read More

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December 2018
4.880 Impact Factor

Analytical validation of a conventional cardiac troponin I assay for dogs and cats.

Vet Clin Pathol 2018 Dec 11. Epub 2018 Dec 11.

Department of Veterinary Clinical Sciences, University of Copenhagen, Frederiksberg C, Denmark.

Background: Cardiac troponins are gold-standard biomarkers of myocardial injury. There is a need for validation of assays with higher availability and lower costs in veterinary medicine.

Objectives: The primary aim of the present study was to perform an analytical validation of the IMMULITE 2000 TnI assay for use in dogs and cats. Read More

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December 2018

Highly sensitive spectrofluorimetric method for rapid determination of orciprenaline in biological fluids and pharmaceuticals.

Luminescence 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura University, Mansoura, Egypt.

Orciprenaline sulphate (ORP) is a direct-acting sympathomimetic with mainly beta-adrenoceptor stimulant activity. It is used as a bronchodilator in the management of reversible airway obstruction. For the first time, a rapid highly sensitive spectrofluorimetric method is described that is relied on measuring the fluorescence spectra of ORP at acidic pH and without addition of any chemical reagents. Read More

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December 2018

Diacylglycerol kinase α-selective inhibitors induce apoptosis and reduce viability of melanoma and several other cancer cell lines.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan.

Diacylglycerol (DG) kinase (DGK), which phosphorylates DG to generate phosphatidic acid (PA), consists of ten isozymes (α-к). Recently, we identified a novel small molecule inhibitor, CU-3, that selectively inhibits the activity of the α isozyme. In addition, we newly obtained Compound A, which selectively and strongly inhibits type I DGKs (α, β, and γ). Read More

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December 2018

Quantification of pentaerythritol tetrastearate- a mold release agent used in polycarbonate by non-aqueous reversed phase HPLC method.

J Sep Sci 2018 Dec 7. Epub 2018 Dec 7.

Analytical Technology, SABIC Technology Center, Bengaluru, Karnataka, India.

A new non-aqueous HPLC method for the quantitative determination of mold release agent pentaerythritol tetrastearate in polycarbonate matrix has been developed and validated. The analyte calibration curve was linear over range of 0.05 - 0. Read More

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December 2018

Compositional elucidation of heavy petroleum base oil by GC×GC-EI/PI/CI/FI-TOFMS.

J Mass Spectrom 2018 Dec 7. Epub 2018 Dec 7.

University of Liège, Department of Chemistry, Organic & Biological Analytical Chemistry Group, Allée du 6 aout, B6c, B-4000, Liège (Sart Tilman), Belgium.

Comprehensive two-dimensional gas chromatography (GC×GC) coupled to time-of-flight mass spectrometry is a powerful separation tool for complex petroleum product analysis. However, the most commonly used electron ionization (EI) technique often makes the identification of the majority of hydrocarbons impossible due to the exhaustive fragmentation and lack of molecular ion preservation, prompting the need of soft-ionization energies. In this study, three different soft-ionization techniques including photo ionization (PI), chemical ionization (CI), and field ionization (FI) were compared against EI to elucidate their relative capabilities to reveal different base oil hydrocarbon classes. Read More

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December 2018

Determination of ospemifene in human plasma by LC-MS/MS and its application to a human pharmacokinetic study.

Biomed Chromatogr 2018 Dec 7:e4451. Epub 2018 Dec 7.

Department of Chemistry, GITAM University, Hyderabad, India.

A highly sensitive, specific and rapid Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analytical method has been developed and validated for the determination of ospemifene in human plasma using ospemifene-d as an internal standard. Solid phase extraction (SPE) technique with Phenomenex Strata X-33μM polymeric sorbent cartridges (30 mg/1ml) was used to extract the analytes from the plasma. The chromatographic separation was achieved on Agilent Eclipse XDB-Phenyl, 4. Read More

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December 2018

Simultaneous quantification of oxybutynin and its active metabolite N-desethyl oxybutynin in rat plasma by ultra high performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study of oxybutynin transdermal patch.

Biomed Chromatogr 2018 Dec 7:e4456. Epub 2018 Dec 7.

Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.

A rapid, selective and sensitive ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously determine oxybutynin (OB) and its active metabolite N-desethyl oxybutynin (DEOB) in rat plasma. Sample of 0.1 mL plasma was extracted with n-hexane. Read More

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December 2018