16,120 results match your criteria Analytical And Bioanalytical Chemistry[Journal]


Biomass-derived carbon dots as a sensitive and selective dual detection platform for fluoroquinolones and tetracyclines.

Anal Bioanal Chem 2022 May 17. Epub 2022 May 17.

School of Chemical Sciences, Mahatma Gandhi University, Priyadarsini Hills P. O., Kottayam, 686 560, Kerala, India.

A novel carbon dot (CD) was synthesized through the facile and simple hydrothermal method from Curcuma amada, as the precursor for the first time. These CDs with an average diameter of 4.6 nm display blue fluorescence, with excitation/emission maxima at 360/445 nm and a quantum yield of 14. Read More

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An SPR-based method for Hill coefficient measurements: the case of insulin-degrading enzyme.

Anal Bioanal Chem 2022 May 16. Epub 2022 May 16.

Chemical Science Department, University of Catania, Viale Andrea Doria 6, Catania, 95125, Italy.

Insulin-degrading enzyme (IDE) is a highly conserved zinc metallopeptidase and is capable to catalytically cleave several substrates besides insulin, playing a pivotal role in several different biochemical pathways. Although its mechanism of action has been widely investigated, many conundrums still remain, hindering the possibility to rationally design specific modulators which could have important therapeutical applications in several diseases such as diabetes and Alzheimer's disease. In this scenario, we have developed a novel surface plasmon resonance (SPR) method which allows for directly measuring the enzyme cooperativity for the binding of insulin in the presence of different IDE activity modulators: carnosine, ATP, and EDTA. Read More

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Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay.

Anal Bioanal Chem 2022 May 16. Epub 2022 May 16.

School of Clinical Medicine, Fujian Medical University, Fuzhou, 350004, Fujian, China.

As a kind of sensing and imaging fluorescent probe with the merit of low toxicity, good stability, and environment-friendly, silicon nanoparticles (SiNPs) are currently attracting extensive research. In this work, we obtained mitoxantrone-SiNPs (MXT-SiNPs) with green emission by one-pot synthesis under mild temperature condition. The antenna based on pyridoxal phosphate (PLP) was designed for light-harvesting to enhance the luminescence of MXT-SiNPs and to establish a novel sensing strategy for alkaline phosphatase (ALP). Read More

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BODIPY-based fluorescent chemosensor for phosgene detection: confocal imaging of nasal mucosa and lung samples from mouse exposed to phosgene.

Anal Bioanal Chem 2022 May 14. Epub 2022 May 14.

School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.

The improper use of phosgene, either as a chemical warfare agent or a leak during chemical production, causes significant risks to human life and property. Therefore, it is particularly important to develop a rapid and highly selective method for the detection of phosgene. In this article, a highly selective fluorescent sensor ONB with a BODIPY unit as a fluorophore and o-aminophenol as a reactive site was constructed for the selective and rapid detection of phosgene in solution. Read More

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An effective long-wavelength fluorescent sensor for Cu based on dibenzylidenehydrazine-bridged biphenylacrylonitrile.

Anal Bioanal Chem 2022 May 14. Epub 2022 May 14.

College of Chemistry and Materials, Fujian Normal University, Fuzhou, 350007, People's Republic of China.

Although numerous fluorescence sensors for Cu have been presented, a long-wavelength sensor in aqueous media has rarely been reported as expected due to practical application requirements. In this work, a novel AIE molecule (DHBB) containing two biphenylacrylonitrile units bridged by dibenzylidenehydrazine was prepared. It possessed the merits of long-wavelength emission, good emission in aqueous media, and multiple functional groups for binding Cu. Read More

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A visual chiroptical system with chiral assembly graphene quantum dots for D-phenylalanine detection.

Anal Bioanal Chem 2022 May 14. Epub 2022 May 14.

Beijing Technology and Business University (BTBU), No. 11 Fucheng Road, Beijing, 100048, People's Republic of China.

Chirality is a fundamental phenomenon of nature, and the enantioselective recognition of amino acids isomers is especially important for life science. In this study, chiroptical system based on chiral assembly graphene quantum dots (GQDs) was developed for visual testing of D-phenylalanine (D-Phe). Here, GQDs were used as the fluorescent element, and chiral functional moieties of 1,3,5-triformylphloroglucinol-functionalized chiral ( +)-diacetyl-L-tartaric anhydride (TPTA) were used as the chiral recognition elements. Read More

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Determination of trace elements in placenta by total reflection X-ray fluorescence spectrometry: effects of sampling and sample preparation.

Anal Bioanal Chem 2022 May 13. Epub 2022 May 13.

Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.

Placental elemental composition can serve as an indicator for neonatal health. Medical studies aiming at revealing such cause-and-effect relationships or studies monitoring potential environmental influences consist of large sample series to ensure statistically sufficient data. Several analytical techniques have been used to study trace metals in human placenta. Read More

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Fluorescence signal of proteins in birch pollen distorted within its native matrix: Identification of the fluorescence suppressor quercetin-3-O-sophoroside.

Anal Bioanal Chem 2022 May 12. Epub 2022 May 12.

Institute of Materials Chemistry, TU Wien, 1060, Vienna, Austria.

The properties of biogenic aerosol strongly depend on the particle's proteinaceous compounds. Proteins from primary biological aerosol particles (PBAPs) can cause allergic reactions in the human respiratory system or act as ice and condensation nuclei in clouds. Consequently, these particles have high impact on human health and climate. Read More

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Separation and characterization of sulfonates in dissolved organic matter from industrial wastewater by solid phase extraction and high-resolution mass spectrometry.

Anal Bioanal Chem 2022 May 12. Epub 2022 May 12.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.

Electrospray ionization (ESI) source combined with high-resolution mass spectrometry (HRMS) has been successfully used to characterize the molecular composition of dissolved organic matter (DOM) in industrial wastewaters. However, sulfonates are commonly presenting in these wastewaters and showing strong ionization suppression for the ESI analysis, which seriously affects the characterization of other DOM components. In this paper, a novel method based on polymer anion exchange (PAX) solid phase extraction (SPE) was proposed to separate DOMs from petroleum refining and coal coking wastewaters into hydrophobic neutral, hydrophobic acids, and hydrophobic strong acids; the fractions were analyzed by negative-ion ESI Fourier transform ion cyclotron resonance mass spectrometry. Read More

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From sea salt to seawater: a novel approach for the production of water CRMs.

Anal Bioanal Chem 2022 May 12. Epub 2022 May 12.

National Research Council Canada, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada.

Natural water certified reference materials (CRMs) are mostly available in a liquid form, and they are produced starting from suitable environmental samples. Many precautions are usually needed to avoid biological or physical degradation, including filtration, acidification, and sterilization. In this study, the drawbacks associated with liquid-based seawater CRMs were tackled by developing a salt-based seawater proxy for nutrients that could be reconstituted in water solution just before use. Read More

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Comprehensive profiling and kinetic studies of glycated lysine residues in human serum albumin.

Anal Bioanal Chem 2022 May 11. Epub 2022 May 11.

Department of Pathology & Anatomical Sciences, School of Medicine, University of Missouri, 1 Hospital Drive, Columbia, MO, 65212, USA.

Lysine residues of proteins slowly react with glucose forming Amadori products. In hyperglycemic conditions, such as diabetes mellitus, this non-enzymatic glycation becomes more pervasive causing severe medical complications. The structure and conformation of a protein predisposes lysine sites to differing reactivity influenced by their steric availability and amino acid microenvironment. Read More

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The interfacial interactions of nanomaterials with human serum albumin.

Anal Bioanal Chem 2022 May 11. Epub 2022 May 11.

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, 116023, China.

The fates of nanomaterials (NMs) in vivo are greatly dependent on their interactions with human serum proteins. However, the interfacial molecular details of NMs-serum proteins are still difficult to be probed. Herein, the molecular interaction details of human serum albumin (HSA) with Au and SiO nanoparticles have been systematically interrogated and compared by using lysine reactivity profiling mass spectrometry (LRP-MS). Read More

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Accumulation and penetration behavior of hypericin in glioma tumor spheroids studied by fluorescence microscopy and confocal fluorescence lifetime imaging microscopy.

Anal Bioanal Chem 2022 May 10. Epub 2022 May 10.

Process Analysis and Technology (PA&T), Reutlingen University, Alteburgstr. 150, 72762, Reutlingen, Germany.

Glioblastoma WHO IV belongs to a group of brain tumors that are still incurable. A promising treatment approach applies photodynamic therapy (PDT) with hypericin as a photosensitizer. To generate a comprehensive understanding of the photosensitizer-tumor interactions, the first part of our study is focused on investigating the distribution and penetration behavior of hypericin in glioma cell spheroids by fluorescence microscopy. Read More

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Supercritical fluid chromatography-tandem mass spectrometry of oxygen heterocyclic compounds in Citrus essential oils.

Anal Bioanal Chem 2022 May 10. Epub 2022 May 10.

Department of Mathematical and Computer Science, Physical Sciences and Earth Sciences, University of Messina, Messina, Italy.

Oxygen heterocyclic compounds are secondary metabolites mainly present in the non-volatile fraction of cold-pressed Citrus essential oils. Under this denomination are included coumarins, furocoumarins, and polymethoxyflavones. These compounds possess numerous beneficial properties for human health, but the ingestion of large amounts of coumarins is often related to toxic effects, whereas the phototoxicity caused by furocoumarins and UVA exposure has been well known for a long time. Read More

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CuO/Au heterostructure dendrimer anchored on Cu foam as dual functional catalytic nanozyme for glucose sensing by enzyme mimic cascade reaction.

Anal Bioanal Chem 2022 May 10. Epub 2022 May 10.

College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China.

Multifunctional catalytic performance plays a crucial role in bio-applications through the diversity and durability of artificial nanozymes. An effective synergy with sufficient accessible active sites and high specific surface area is a challenge for composite catalysts, especially to avoid uncontrollable aggregation and structural instability. Here, we fabricated a CuO/Au heterostructure dendrimer on copper foam (CuO/Au HD/CF) as dual functional catalytic nanozyme to achieve enzyme mimic cascade reactions for efficient colorimetric analysis. Read More

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A novel galactose electrochemical biosensor intended for point-of-care measurement of quantitative liver function using galactose single-point test.

Anal Bioanal Chem 2022 May 7. Epub 2022 May 7.

Taipei Medical University, Taipei, Taiwan, Republic of China.

Liver disease has emerged as a healthcare burden because of high hospitalization rates attributed both to steatohepatitis and to severe hepatic toxicity associated with changes of drug exposure. Early detection of hepatic insufficiency is critical to preventing long-term liver damage. The galactose single-point test is recommended by the US FDA as a sensitive means to quantify liver function, yet the conventional method used for quantitation of circulating galactose still relies on the standard colorimetric method, requiring time-consuming and labor-intensive processes, and is confined to the medical laboratory, thus limiting prevalence. Read More

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CeO2/[email protected] hollow carbon microspheres with improved peroxidase-like activity for the determination of quercetin.

Anal Bioanal Chem 2022 May 6. Epub 2022 May 6.

College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, People's Republic of China.

Nanozymes, as substitutes for natural enzymes, have been used to construct some fast cheap sensing platforms by virtue of the high catalytic activity. Herein, we firstly study the peroxidase-like activity of CeO/[email protected] hollow carbon microspheres as nanozymes. In the presence of HO, CeO/[email protected] exhibits high peroxidase-like activity evaluated by the catalytic oxidation of the chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB) into a blue oxTMB visually in 1 min. Read More

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An efficient aggregation-induced electrochemiluminescent immunosensor by using TiO nanoparticles as coreaction accelerator and energy donor for aflatoxin B detection.

Anal Bioanal Chem 2022 May 6. Epub 2022 May 6.

State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, Shandong, China.

Herein, we fabricated a label-free ECL immunosensor for aflatoxin B (AFB) detection. In this system, a small organic aggregation-induced electrochemiluminescence luminophore, 2,5-di-tetraphenylethylene-ylthiazolo [5,4-d] thiazole, was designed, named TPETTZ. Polyaniline-wrapped TiO nanoparticles (PANI/TiO NPs) complex was synthesized through one-step in situ oxidation polymerization of aniline, and performed excellent electrical conductivity and abundant amino groups. Read More

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A sustainable approach for the stability study of psychotropic substances using vitreous humor and liver as alternative matrices.

Anal Bioanal Chem 2022 May 5. Epub 2022 May 5.

Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387, Kraków, Poland.

The stability of psychotropic substances representing various drug groups important from the perspective of forensic chemistry, including benzodiazepines, antidepressants, carbamazepine, cocaine, and their selected metabolites, was investigated for 1 month in two alternative biological matrices, vitreous humor and liver homogenate. Three different thermal storage conditions (-20, 4, and 20 °C) were tested. Liquid chromatography-mass spectrometry (LC-MS) analysis was preceded by an effective solid-phase microextraction (SPME) procedure. Read More

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Sample preparation optimization of insects and zebrafish for whole-body mass spectrometry imaging.

Anal Bioanal Chem 2022 May 4. Epub 2022 May 4.

Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong, China.

Appropriate sample preparation is one of the most critical steps in mass spectrometry imaging (MSI), which is closely associated with reproducible and reliable images. Despite that model insects and organisms have been widely used in various research fields, including toxicology, drug discovery, disease models, and neurobiology, a systematic investigation on sample preparation optimization for MSI analysis has been relatively rare. Unlike mammalian tissues with satisfactory homogeneity, freezing sectioning of the whole body of insects is still challenging because some insect tissues are hard on the outside and soft on the inside, especially for some small and fragile insects. Read More

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A chemiluminescence aptasensor for sensitive detection of alpha-fetoprotein based on [email protected]

Anal Bioanal Chem 2022 May 4. Epub 2022 May 4.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.

In this work, [email protected] composites were prepared and were used to construct a chemiluminescence (CL) aptasensor for alpha-fetoprotein (AFP) detection. Hemin is a catalytic porphyrin with two carboxylate groups that can covalently bond to metal ions. A hemin/ZIF-67 composite was prepared via covalent bonding between the carboxyl groups of hemin and the cobalt ion of ZIF-67, and these materials were characterized by scanning electron microscopy (SEM), infrared spectroscopy (IR), and X-ray diffraction (XRD). Read More

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Estimation of the concentrations of hydroxylated polychlorinated biphenyls in human serum using ionization efficiency prediction for electrospray.

Anal Bioanal Chem 2022 May 4. Epub 2022 May 4.

Department of Materials and Environmental Science, Stockholm University, Svante Arrhenius väg 16, 106 91, Stockholm, Sweden.

Hydroxylated PCBs are an important class of metabolites of the widely distributed environmental contaminants polychlorinated biphenyls (PCBs). However, the absence of authentic standards is often a limitation when subject to detection, identification, and quantification. Recently, new strategies to quantify compounds detected with non-targeted LC/ESI/HRMS based on predicted ionization efficiency values have emerged. Read More

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Impact of instrumental settings in electrospray ionization ion trap mass spectrometry on the analysis of O-methoxyethyl-O-methyl cellulose: a comprehensive quantitative evaluation.

Anal Bioanal Chem 2022 May 3. Epub 2022 May 3.

Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstr 20, 38106, Braunschweig, Germany.

The hydroxyethyl substitution along and among the polymer chains of respective cellulose ethers (HEC and HEMC) can be analyzed by ESI-IT-MS after permethylation of the free OH-groups, partial hydrolysis, and mABA labeling. This method requires the correct quantification of the molar ratios of the constituents belonging to a particular degree of polymerization (DP) with respect to their numbers of MeOEt and Me groups without any discrimination along the MS analysis pathway. The influence of the chemistry on the ionization and the impact of the voltages controlling the ion transport (Cap Exit, Octopoles) and the ion storage efficiency (Trap Drive, TD) on a relative quantification were studied using binary equimolar mixtures of cellobiose with increasing number of methoxyethyl and decreasing number of methyl groups (Δ m/z 88, 2× MeOEt). Read More

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Rapid and simple detection of influenza virus via isothermal amplification lateral flow assay.

Anal Bioanal Chem 2022 May 2. Epub 2022 May 2.

Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea.

Respiratory illness caused by influenza virus is a serious public health problem worldwide. As the symptoms of influenza virus infection are similar to those of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, it is essential to distinguish these two viruses. Therefore, to properly respond to a pathogen, a detection method that is capable of rapid and accurate diagnosis in a hospital or at home is required. Read More

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Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC-MS/MS analysis.

Anal Bioanal Chem 2022 May 2. Epub 2022 May 2.

Bioanalytical Chemistry, Humboldt University Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany.

The determination of vitamin D metabolites as status marker or for diagnostic purposes is almost entirely conducted from blood serum or plasma. Other biological matrices, however, have also interested researchers, for two main reasons: (1) alternative matrices may allow non-invasive sampling, permit easier sample transfer and require less demanding storage conditions; and (2) the levels of vitamin D metabolites in other body compartments may further aid the understanding of vitamin D metabolism and function. Thus, the development of reliable and efficient sample preparation protocols for sample matrices other than serum/plasma, which will remove potential interferences and selectively extract the targeted metabolites, is of great importance. Read More

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Flow-modulated comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry: use of hydrogen as a more sustainable alternative to helium.

Anal Bioanal Chem 2022 Apr 30. Epub 2022 Apr 30.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.

The present research is focused on the use and evaluation of hydrogen, as a more sustainable alternative to helium, within the context of fast flow modulation comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. In such a respect, a comparison was made between the two mobile phases in terms of speed and overall chromatography performance. All experiments were carried out by using the following column set: low polarity with dimensions 10 m × 0. Read More

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Analytical methods and applications in materials and life sciences.

Anal Bioanal Chem 2022 Apr 30. Epub 2022 Apr 30.

Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.

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Analysis of Ti- and Pb-based particles in the aqueous environment of Melbourne (Australia) via single particle ICP-MS.

Anal Bioanal Chem 2022 Apr 28. Epub 2022 Apr 28.

Institute of Chemistry, University of Graz, 8010, Graz, Austria.

The analysis of natural and anthropogenic nanomaterials (NMs) in the environment is challenging and requires methods capable to identify and characterise structures on the nanoscale regarding particle number concentrations (PNCs), elemental composition, size, and mass distributions. In this study, we employed single particle inductively coupled plasma-mass spectrometry (SP ICP-MS) to investigate the occurrence of NMs in the Melbourne area (Australia) across 63 locations. Poisson statistics were used to discriminate between signals from nanoparticulate matter and ionic background. Read More

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A proteomics strategy for the identification of multiple sites in sulfur mustard-modified HSA and screening potential biomarkers for retrospective analysis of exposed human plasma.

Anal Bioanal Chem 2022 Apr 27. Epub 2022 Apr 27.

State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's Republic of China.

A major challenge for the unequivocal verification of alleged exposure to sulfur mustard (HD) lies in identifying its multiple modifications on endogenous proteins and utilizing these modified proteins to achieve accurate, sensitive, and rapid detection for retrospective analysis of HD exposure. As the most abundant protein in human plasma, human serum albumin (HSA) can react with many xenobiotics, such as HD, to protect the body from damage. The HSA adducts induced by HD have been used as biomarkers for the verification of HD exposure. Read More

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Target amplification-free detection of urinary microRNA for diabetic nephropathy diagnosis with electrocatalytic reaction.

Anal Bioanal Chem 2022 Apr 27. Epub 2022 Apr 27.

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bang Khun Thian Campus, Bangkok, 10150, Thailand.

Diabetic nephropathy (DN) is a serious diabetic complication, usually developed from type II diabetes mellitus (T2DM) and known as type II DN (T2DN). New emerging biomarkers for T2DN are microRNAs (miRNAs) which have been studied for the noninvasive early-stage detection of the disease. In this work, a nucleic acid amplification-free miRNA-124 sensor based on target-induced strand displacement on magnetic beads, and by using methylene blue-loaded silica particles as a label was developed. Read More

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