12,619 results match your criteria Analytica Chimica Acta[Journal]

In-situ preparation of hollow CdCoS heterojunction with enhanced photocurrent response for highly photoelectrochemical sensing of organophosphorus pesticides.

Anal Chim Acta 2022 Jun 10;1212:339913. Epub 2022 May 10.

Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong, 515063, PR China; Analysis & Testing Center, Shantou University, Shantou, Guangdong, 515063, PR China. Electronic address:

In this study, a porous hollow CdCoS(2) microsphere was synthesized based on the ZIF-67-S MOFs derived method of sulfurization reaction and calcination process. Under visible light irradiation, the resulting CdCoS(2) composite showed a markedly enhanced photoelectrochemical (PEC) response. The photocurrent value of the CdCoS(2) modified ITO electrode was 93-fold and 41-fold than that of CoS and CdS materials, respectively. Read More

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Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.

Anal Chim Acta 2022 Jun 14;1212:339942. Epub 2022 May 14.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL, 32306, USA. Electronic address:

Insulin secreted from islets of Langerhans is the main hormone to reduce blood glucose. Examination of insulin secretion patterns at the single islet level reveals functional differences in the timings and patterns of release. This heterogeneous response highlights the importance of developing systems to measure dynamic release from small numbers of islets in parallel. Read More

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Sensitive detection of exosomes by gold nanoparticles labeling inductively coupled plasma mass spectrometry based on cholesterol recognition and rolling circle amplification.

Anal Chim Acta 2022 Jun 13;1212:339938. Epub 2022 May 13.

Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

Exosomes, potential biomarkers for liquid biopsy, provide a promising opportunity for early cancer diagnosis in a noninvasive manner. However, direct analysis of exosomes in complicated biological samples is still challenging. Herein, a cholesterol recognition assay was proposed for detection of exosomes by gold nanoparticles (Au NPs) labeling inductively coupled plasma mass spectrometry. Read More

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VIR-CRISPR: Visual in-one-tube ultrafast RT-PCR and CRISPR method for instant SARS-CoV-2 detection.

Anal Chim Acta 2022 Jun 13;1212:339937. Epub 2022 May 13.

Clinical Laboratory, Linping Campus, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 311100, China; School of Life Sciences, Fudan University, Shanghai, 200438, China. Electronic address:

Until now, corona virus disease 2019 (COVID-19) remained to be an enormous threat for global health. As one viral illness induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), versatile, rapid and sensitive method for SARS-CoV-2 detection in early stage is urgently needed. Here, we reported an ultrasensitive and visual in-one-tube detection method which could be accomplished within half an hour from sampling-to-result. Read More

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Simultaneous measurements of Cr, Cd, Hg and Pb species in ng L levels by interfacing high performance liquid chromatography and inductively coupled plasma mass spectrometry.

Anal Chim Acta 2022 Jun 13;1212:339935. Epub 2022 May 13.

College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address:

Toxicity, mobility, bioavailability and biofunctions of chromium, cadmium, mercury and lead are heavily dependent upon their specific chemical forms, leading to a high demand to metal speciation analysis rather than total quantification. Simultaneous speciation analysis of multiple metal(loid)s is attractive to a large sample capacity containing unstable analytes due to its economic and environmental advantages over the conventional single elemental strategies. In this work, an analytical method integrating online solid phase extraction into high performance liquid chromatography interfaced with inductively coupled plasma mass spectrometry (ICP-MS) to simultaneously preconcentrate and quantify Cr, Cd, Hg and Pb forms in pg L levels in water was developed. Read More

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A novel methyl-dependent DNA endonuclease GlaI coupling with double cascaded strand displacement amplification and CRISPR/Cas12a for ultra-sensitive detection of DNA methylation.

Anal Chim Acta 2022 Jun 8;1212:339914. Epub 2022 May 8.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; National Facility for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address:

Detection of methylation changes associated with oncogenic transformation is essential for early screening and treatment of cancer. Herein, we propose a novel DNA methylation detection assay based on the methyl-dependent DNA endonuclease GlaI coupling with double cascaded strand displacement amplification and CRISPR/Cas12a (GlaI-DC-SDA-CRISPR/Cas12a). The GlaI enables highly specific recognition and digestion of methylated target site (dsDNA) but leaves unmethylated target intact. Read More

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Wearable transdermal colorimetric microneedle patch for Uric acid monitoring based on peroxidase-like polypyrrole nanoparticles.

Anal Chim Acta 2022 Jun 7;1212:339911. Epub 2022 May 7.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address:

Regular Uric Acid (UA) monitoring is essential for patients with gout, and great efforts have been made to develop portable, low-cost and easy-to-operate UA detection technologies. Herein, we developed a wearable microneedle colorimetric patch integrating the sampling function and the capability of real-time UA analysis in a minimally invasive manner, which can dramatically improve patient compliance. For the operation of the constructed device, the poly(vinyl alcohol) based microneedle was embedded with the uricase which catalyzed the oxidation of UA extracted from the interstitial fluids and produced HO. Read More

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In situ spatiotemporal solute imaging of metal corrosion on the example of magnesium.

Anal Chim Acta 2022 Jun 6;1212:339910. Epub 2022 May 6.

Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry, Montanuniversität Leoben, Franz-Josef-Strasse 18, 8700, Leoben, Austria. Electronic address:

Visualization and quantification of corrosion processes is essential in materials research. Here we present a new approach for 2D spatiotemporal imaging of metal corrosion dynamics in situ. The approach combines time-integrated Mg flux imaging by diffusive gradients in thin films laser ablation inductively coupled plasma mass spectrometry (DGT LA-ICP-MS) and near real-time pH imaging by planar optodes. Read More

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Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription.

Anal Chim Acta 2022 Jun 6;1212:339909. Epub 2022 May 6.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05902, Republic of Korea. Electronic address:

Diagnosis of SARS-CoV-2 infection through rapid, accurate, and sensitive testing is the most important and fundamental step in coping with the COVID-19 epidemic. We have developed a sensitive fluorometric assay to detect SARS-CoV-2 viral RNA without thermal cycling. This assay system, based on tandem isothermal gene amplification (TIGA), is composed of ternary rolling circle amplification (t-RCA) and subsequent strand displacement amplification (SDA) coupled with G-quadruplex-generating RCA (SDA/GQ-RCA). Read More

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Silver-fluoropolymer (Ag-CF) films: Kinetic study of silver release, and spectroscopic-microscopic insight into the inhibition of P. fluorescens biofilm formation.

Anal Chim Acta 2022 Jun 5;1212:339892. Epub 2022 May 5.

Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert Einstein Allee, 11, 89081, Ulm, Germany. Electronic address:

Silver-fluoropolymer (Ag-CF) composed of encapsulated bioactive nanophases within a thin polymer coating are promising antimicrobial films with excellent bioactivity. In this contribution, we report on Ag-CF thin films obtained by ion beam co-sputtering, accurately tuning film thickness, and inorganic loading. The Ag-CF films were characterized by spectroscopic and scanning probe microscopy techniques with respect to composition and swelling behavior. Read More

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New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS.

Anal Chim Acta 2022 Jun 2;1212:339891. Epub 2022 May 2.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') by gas chromatography-mass spectrometry. Read More

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Widely quasi-quantitative analysis enables temporal bile acids-targeted metabolomics in rat after oral administration of ursodeoxycholic acid.

Anal Chim Acta 2022 Jun 10;1212:339885. Epub 2022 May 10.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China. Electronic address:

Bile acid (BA) pool homeostasis can be disturbed when cholestasis, and ursodeoxycholic acid (UDCA) treatment is able to rectify the perturbed pool. Efforts were made here to propose a strategy enabling BAs-focused widely quantitative metabolomics and subsequently to lucubrate BA pool fluctuation trajectory in rats after dosing UDCA. A so-called universal metabolome standard (UMS) sample containing numerous natural BAs was constructed by involving various available BAs-enriched matrices. Read More

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Electrochemical biosensors based on nanomaterials for aflatoxins detection: A review (2015-2021).

Anal Chim Acta 2022 Jun 26;1212:339658. Epub 2022 Feb 26.

NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain; Biotechnology Institute of Asturias, University of Oviedo, Santiago Gascon Building, 33006, Oviedo, Spain. Electronic address:

Due to their immunotoxicity, aflatoxins are considered as very important natural contaminants of a wide variety of agricultural products. Although conventional techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) combined with mass spectrometry (MS) have traditionally been used to detect such food contaminants, they are relatively expensive, laborious, and time consuming which limits their use for in field analysis at the so-called point-of-care (POC). Electrochemical biosensors are emerging devices that meet these expectations since they rely in reliable, simple, inexpensive, portable, selective, and easy-to-use analytical procedures and instruments that can be used by unskilled personnel outside the laboratory. Read More

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Antifouling ionic liquid doped molecularly imprinted polymer-based ratiometric electrochemical sensor for highly stable and selective detection of zearalenone.

Anal Chim Acta 2022 Jun 30;1210:339884. Epub 2022 Apr 30.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China. Electronic address:

Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin, and its accurate detection in complex biological samples is still a challenge. Herein, an antifouling ratiometric electrochemical sensor has been developed for its detection. In this system, black phosphorus-graphene oxide is used as substrate to amplify the signal; ionic liquid doped molecularly imprinted polymer (MIP) endows the sensing surface not only high recognition ability but also high capability to resist nonspecific adsorption. Read More

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Quenching of tungsten-based polyoxometalate nanoclusters on electrochemiluminescence emission of luminol loaded CeVO/Au for immunoassay of protein.

Anal Chim Acta 2022 Jun 29;1210:339883. Epub 2022 Apr 29.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China. Electronic address:

A quenching-typed electrochemiluminescence (ECL) immunosensing method was proposed for protein detection by the redox reaction of tungsten-based polyoxometalate nanoclusters (W-POM NCs) with free radical (O) as the intermediate of co-reactant HO. The immunosensor was constructed by co-immobilizing luminol loaded CeVO/Au nanohybrids and capture antibody on an indium tin oxide electrode, which showed strong ECL emission in the presence of HO due to the catalysis of CeVO/Au toward its electro-oxidation to produce O via the reversible conversion between Ce and Ce. The W-POM NCs were encapsulated in the uniformly sized amino-silica nanosphere (SiO) to act as a label ([email protected]) of the secondary antibody. Read More

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Sweat urea bioassay based on degradation of Prussian Blue as the sensing architecture.

Anal Chim Acta 2022 Jun 29;1210:339882. Epub 2022 Apr 29.

Department of Pharmacy, University of Naples "Federico II", Via D. Montesano 49, 80131, Naples, Italy; BAT Center - Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055, Naples, Italy. Electronic address:

Urea provides pathophysiological information on renal and hepatic disorders. Among the secretion pathways via several bio-fluids, sweat urea represent an important and non-invasive strategy to evaluate kidney and liver pathologies. In this work, a rapid and reliable colorimetric urea bioassay assisted by chemometrics design of experiments has been obtained. Read More

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pH-guided fluorescent sensing probe for the discriminative detection of Cl and Br in human serum.

Anal Chim Acta 2022 Jun 27;1210:339879. Epub 2022 Apr 27.

Department of Chemistry, Gwangju Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, Republic of Korea. Electronic address:

The fluctuation of the halide ion concentration in human serum is of a high clinical implication. The measurement of Cl in human serum samples is important because Cl is a major constituent of human serum and certain Cl levels are indicators of many acute diseases. Real-time monitoring of Br levels in human serum samples from patients ingesting Br salt-based antiepileptic drugs is important to regulate potential adverse drug effects. Read More

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Quantification of immunosuppressants from one 3.2 mm dried blood spot by a novel cold-induced phase separation based LC-MS/MS method.

Anal Chim Acta 2022 Jun 1;1210:339889. Epub 2022 May 1.

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address:

Dried blood spots (DBS) have been regarded as a promising alternative for therapeutic drug monitoring (TDM) of immunosuppressants (ISDs) for over fifteen years. Nonetheless, there are still three main issues impeding its preference: (i) the requirement of relatively large disc; (ii) the controversial and intricate desorption approaches; (iii) the lack of feasible extraction strategies. For improvement, this work described a new LC-MS/MS method realizing quantification of four ISDs from one piece of 3. Read More

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A novel UHPLC-ESI-MS/MS method and automatic calculation software for regiospecific analysis of triacylglycerols in natural fats and oils.

Anal Chim Acta 2022 Jun 1;1210:339887. Epub 2022 May 1.

Food Chemistry and Food Development, Department of Life Technologies, FI-20500, University of Turku, Turku, Finland. Electronic address:

Regioisomeric analysis of triacylglycerols (TAGs) in natural oils and fats is a highly challenging task in analytical chemistry. Here we present a software (TAG Analyzer) for automatic calculation of regioisomeric composition of TAGs based on the mass spectral data from recently reported ultra-high performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method for analyzing TAG regioisomers. The software enables fast and accurate processing of complex product ion spectra containing structurally informative diacylglycerol [M+NH-RCOH-NH] and fatty acid ketene [RCO] fragment ions. Read More

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Trapped ion mobility spectrometry-mass spectrometry improves the coverage and accuracy of four-dimensional untargeted lipidomics.

Anal Chim Acta 2022 Jun 2;1210:339886. Epub 2022 May 2.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, PR China. Electronic address:

Lipids play vital roles in many physiological and pathological processes in living organisms. Due to the high structural diversity and the numerous isomers and isobars of lipids, high-coverage and high-accuracy lipidomic analysis of complex biological samples remain the bottleneck to investigate lipid metabolism. Here, we developed the trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) based four-dimensional untargeted lipidomics to support accurate lipid identification and quantification in biological samples. Read More

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Quantification of endocannabinoids in human cerebrospinal fluid using a novel micro-flow liquid chromatography-mass spectrometry method.

Anal Chim Acta 2022 Jun 2;1210:339888. Epub 2022 May 2.

Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands. Electronic address:

The endocannabinoid system (ECS) is implicated in various brain disorders. Changes in the composition of the cerebrospinal fluid (CSF) may be associated with ECS-related pathologies. Endocannabinoids (eCBs) and their analogues are present at low concentrations in human CSF, which hampered the investigation of the ECS in this body fluid. Read More

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Multicommutation flow analysis system for non-enzymatic lactate determination based on light-driven photometric assay.

Anal Chim Acta 2022 Jun 27;1210:339878. Epub 2022 Apr 27.

University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093, Warsaw, Poland. Electronic address:

A fully-mechanized bioanalytical system for the photometric light-driven lactate determination is presented. The system is based on highly integrated four diode flow-through cell in which two UV-LEDs are used for the photochemical reduction of Fe(III) in the presence of lactate whereas LED-photodiode system allows the photometric detection of Fe(II)-Ferrozine complex to be registered. The developed multicommutation flow analysis system consisting of bifunctional optoelectronic device (photodegradation of Fe(III)-lactate complex and detection), solenoid micropumps and microvalves, is fully controlled and powered by the Arduino-compatible electronics. Read More

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Renewable sorbent dispersive solid phase extraction automated by Lab-In-Syringe using magnetite-functionalized hydrophilic-lipophilic balanced sorbent coupled online to HPLC for determination of surface water contaminants.

Anal Chim Acta 2022 Jun 26;1210:339874. Epub 2022 Apr 26.

Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Hradec Králové, 500 05, Czech Republic. Electronic address:

An automated methodology for magnetic dispersive solid phase microextraction integrating bead injection approach for renewable sorbent introduction is presented for the first time and was successfully applied to the enrichment of water contaminants. For this purpose, a simple procedure was developed for the functionalization of commercial SupelTM-Select HLB (Hydrophilic modified styrene polymer) sorbent beads that allowed embedding magnetite nanoparticles (FeO). The sorbent was then used in a dispersive solid phase extraction procedure that was carried out entirely inside the void of an automatic syringe pump following the flow-batch concept of Lab-In-Syringe including automated renewal of the sorbent for each analysis. Read More

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BSA-Cu(PO) hybrid nanoflowers as a high-performance redox indicator for robust label-free electrochemical immunoassay.

Anal Chim Acta 2022 Jun 26;1210:339873. Epub 2022 Apr 26.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi, 710127, PR China. Electronic address:

In this work, we report that copper ion-centered hybrid nanoflowers (HNFs) can directly act as high-performance redox indicator via a facile one-step coprecipitation methodology. By altering the bovine serum albumin (BSA) concentration in the coprecipitation synthesis system, the nucleation, growth and electrochemical activities of BSA-Cu(PO) HNFs could be well regulated. Thanks to the intrinsic redox activity and high specific surface area of BSA-Cu(PO) HNFs, it could be utilized not only as the direct electrochemical signal indicator, but also acted as nanocarriers for loading numerous detection antibodies, which was further employed for robust label-free electrochemical immunoassay of α-fetoprotein (AFP). Read More

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A two-dimensional G-CoP/N,P-co-doped carbon nanowire electrode for the simultaneous determination of hydroquinone and catechol in domestic wastewater.

Anal Chim Acta 2022 Jun 28;1210:339871. Epub 2022 Apr 28.

Key Laboratory of Magnetic Molecules and Magnetic Information Materials (Ministry of Education), School of Chemistry and Material Science, Shanxi Normal University, Taiyuan, 030031, China. Electronic address:

Hydroquinone (HQ) and catechol (CC) are important chemical raw materials in the modern industry, unfortunately, which are also high toxic phenolic pollutants. So how to achieve highly sensitive and selective determination HQ and CC is the challenge we face. In the present work, we report a facile strategy to obtain nitrogen and phosphorous co-doped glucose-derived carbon coated CoP nanowires (G-CoP/N,P-C NWs), in which nitrilotriacetic acid (NTA) was as the chelating reagent, glucose was as carbon source, and the precursors were subsequently experienced carbonization and phosphorization process. Read More

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Au-Se bonded nanoprobe for prostate specific antigen detection in serum.

Anal Chim Acta 2022 Jun 25;1210:339852. Epub 2022 Apr 25.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, PR China. Electronic address:

The detection of prostate specific antigen (PSA) in serum can realize early diagnosis of prostate cancer and prevent the occurrence of prostate tumors, as well as offering guidance during the therapy. Herein, a Au-Se bonded nanoprobes that can specifically detect PSA was designed and constructed. The peptide chains that can be specifically cleaved by PSA were firstly functionalized with fluorescent dye and selenol, and then bind to the Au nanoparticles to produce the probe. Read More

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Low and high resolution gas chromatography-mass spectrometry for untargeted metabolomics: A tutorial.

Anal Chim Acta 2022 Jun 9;1210:339043. Epub 2021 Sep 9.

Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, CEU Universities. Campus Monteprincipe, Boadilla Del Monte, 28668, Madrid, Spain. Electronic address:

GC-MS for untargeted metabolomics is a well-established technique. Small molecules and molecules made volatile by derivatization can be measured and those compounds are key players in main biological pathways. This tutorial provides ready-to-use protocols for GC-MS-based metabolomics, using either the well-known low-resolution approach (GC-Q-MS) with nominal mass or the more recent high-resolution approach (GC-QTOF-MS) with accurate mass, discussing their corresponding strengths and limitations. Read More

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Three-dimensional g-CN/MWNTs/GO hybrid electrode as electrochemical sensor for simultaneous determination of ascorbic acid, dopamine and uric acid.

Anal Chim Acta 2022 Jun 4;1211:339907. Epub 2022 May 4.

School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, PR China. Electronic address:

A three-dimensional g-CN/MWNTs/GO hybrid modified electrode was constructed as an electrochemical sensor for the simultaneous determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Due to the high conductivity of MWCNTs and the strong synergy between g-CN and GO, the combination of the three effectively improved the electrocatalytic activity of the modified electrode for the oxidation of AA, DA, and UA, and solved the problems such as overlapping anodic peaks. The electrochemical performance of the as-constructed sensor was investigated and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Read More

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Unravelling error sources in miniaturized NIR spectroscopic measurements: The case study of forages.

Anal Chim Acta 2022 Jun 4;1211:339900. Epub 2022 May 4.

Università degli Studi dell'Insubria, Science and High Technology Department, 22100, Como, Italy. Electronic address:

The use of miniaturized NIR spectrometers is spreading over the scientific literature with a particular focus on developing methods as rapid and easy-to-use as possible and following the philosophy of green analytical chemistry. Several applications and studies are typically presented by comparing results obtained with benchtop instrumentation even when the analytical strategies are substantially different. Indeed, analytical applications that include the use of miniaturized instrumentation are subject to several sources of variability that need to be known at the time of method development. Read More

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Evaluation and optimization of quantitative analysis of cofactors from yeast by liquid chromatography/mass spectrometry.

Anal Chim Acta 2022 Jun 2;1211:339890. Epub 2022 May 2.

Department of Biotechnology, Graduate School and Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, South Korea. Electronic address:

Cofactors play pivotal roles in catabolism and anabolism in all living organisms. Many studies have investigated the concentration of cofactors in living organisms to understand their metabolic status, which can be used to produce valuable chemicals or to understand the pathophysiology of diseases. Among various analytical platforms, liquid chromatography/mass spectrometry (LC/MS) is the most frequently used method for the quantification of cofactors. Read More

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