Anal Chim Acta 2018 Jun 30;1010:86-96. Epub 2017 Dec 30.
Nestlé Research Centre, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
A method for the determination of non-lactose oligosaccharides (NLO) in milk using liquid chromatography has been developed. Oligosaccharides were labelled with a fluorescent tag, 2-aminobenzamide (2AB), and were identified by comparison of their retention times to those of oligosaccharide standards, their mass (as measured by mass spectrometry) and their fragmentation patterns in the mass spectrometer. The concentrations of the NLO in milk have been determined using 2 different approaches: (1) by preparing a calibration curve using genuine standards of each oligosaccharide. Read More