9,833 results match your criteria Analytica Chimica Acta[Journal]


Palindromic molecular beacon-based intramolecular strand-displacement amplification strategy for ultrasensitive detection of K-ras gene.

Anal Chim Acta 2019 Aug 19;1065:98-106. Epub 2019 Mar 19.

State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering Hunan University, Changsha, 410082, PR China.

The sensitive detection of tumor proto-oncogenes is indispensable because the early diagnosis and accurate treatment of genetic diseases is the key guarantee of patients' health. In this study, we proposed a novel palindromic molecular beacon (PMB) that it bases on the signal amplification strategy for ultrasensitive detection of Kras gene codon 12. PMB is designed to have two palindromic fragments at its two ends, one of which is locked via folding into a hairpin structure and the other promotes the formation of PMB duplex via intermolecular self-hybridization. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.059DOI Listing

Non-invasive diagnosis of bladder cancer by detecting telomerase activity in human urine using hybridization chain reaction and dynamic light scattering.

Anal Chim Acta 2019 Aug 20;1065:90-97. Epub 2019 Mar 20.

School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, PR China. Electronic address:

Cystoscopy and histology are the gold standards for detection of bladder cancer. However, these methods are highly subjective, expensive, and invasive. We have developed a non-invasive method for the diagnosis of bladder cancer by detecting telomerase activity in human urine. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.039DOI Listing

Analysis of molecular orientation in polymeric spherulite using polarized micro attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic imaging.

Anal Chim Acta 2019 Aug 19;1065:79-89. Epub 2019 Feb 19.

Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom. Electronic address:

Micro ATR-FTIR spectroscopic imaging enables the visualization of two-dimensional chemical distribution at a higher spatial resolution than macro-transmission FTIR imaging approach. In this study, micro ATR-FTIR imaging was applied for analysis of a specific morphology in a spherulite of poly(3-hydroxybutyrate) (PHB). The PHB spherulites crystallized at an isothermal condition, showed the fine band structure due to the twisting lamellar crystals during the spherulite growth under the polarized optical microscope (POM). Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.017DOI Listing

A low-cost microfluidic platform for rapid and instrument-free detection of whooping cough.

Anal Chim Acta 2019 Aug 5;1065:71-78. Epub 2019 Mar 5.

Department of Chemistry and Biochemistry, USA; Biomedical Engineering, USA; Border Biomedical Research Center, USA; Environmental Science and Engineering, University of Texas at El Paso, 500 West University Ave, El Paso, TX, 79968, USA. Electronic address:

Whooping cough also called Pertussis is a highly contagious respiratory infection that affects all age populations. Given recent pertussis outbreaks, there is an urgent need for a point-of-care (POC) device for rapid diagnosis of pertussis. Herein, we report a low-cost microfluidic POC device integrated with loop-mediated isothermal amplification (LAMP) technique for the rapid and accurate diagnosis of pertussis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193025
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http://dx.doi.org/10.1016/j.aca.2019.03.001DOI Listing
August 2019
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Flow controllable three-dimensional paper-based microfluidic analytical devices fabricated by 3D printing technology.

Anal Chim Acta 2019 Aug 3;1065:64-70. Epub 2019 Mar 3.

Chengdu Institute of Biology, Chinese Academy of Sciences, No. 93 South Keyuan Road, Gaoxin Distinct, Chengdu, 610041, PR China. Electronic address:

In most cases, three-dimensional paper-based microfluidic analytical devices (3D-μPADs) were fabricated manually by stacking or folding methods. For the first time, digital light processing stereolithography (DLP-SLA) 3D printing technology was adopted to automatically make 3D-μPADs. In the fabrication process, a printing pause was set between two layers to allow paper to be placed in the resin tank. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193023
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http://dx.doi.org/10.1016/j.aca.2019.02.046DOI Listing
August 2019
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Single identical cell toxicity assay on coordinately ordered patterns.

Anal Chim Acta 2019 Aug 2;1065:56-63. Epub 2019 Mar 2.

Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, USA. Electronic address:

Toxicity assay is crucial in identifying biological mechanisms, detecting disease and screening therapeutics. Single cell toxicity assay reveals heterogeneous responses of each cell without ensemble average, but still cannot indicate responses of the same individual cells to multiple treatments. This article reports an identical cell assay that can be used at high throughput to locate each cell and track its responses to multiple treatments (chemicals and radiation), thus greatly facilitates toxicity based drug screening and testing. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.040DOI Listing

In situ decomposition of deep eutectic solvent as a novel approach in liquid-liquid microextraction.

Anal Chim Acta 2019 Aug 20;1065:49-55. Epub 2019 Mar 20.

Institute of Chemistry, Saint Petersburg State University, RU-198504, Saint Petersburg, Russia.

In this study, a novel approach for effective liquid-liquid microextraction based on deep eutectic solvent (DES) decomposition was suggested for the first time. It was established that DESs synthesized from tetrabutylammonium bromide and long-chain alcohols decomposed in aqueous phase resulting in in situ dispersion of organic phase and extraction of hydrophobic analyte(s). It this process long-chain alcohol acted as an extraction solvent and tetrabutylammonium bromide acted as a dispersive agent and promoted mass transfer between aqueous and organic phases as a salting out agent. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193032
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http://dx.doi.org/10.1016/j.aca.2019.03.038DOI Listing
August 2019
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Boronate affinity Metal-Organic frameworks for highly efficient cis-diol molecules in-situ enrichment and surface-assisted laser desorption/ionization mass spectrometric detection.

Anal Chim Acta 2019 Aug 21;1065:40-48. Epub 2019 Mar 21.

School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233000, China; Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China. Electronic address:

Development of novel matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a hot-spot in research frontiers. Herein, we established novel SALDI-MS approach for the analysis of cis-diol small molecules by using boronate affinity metal-organic frameworks (BA-MOFs) as the sorbent for extraction and later as matrix for SALDI. In the meantime, the prepared BA-MOFs exhibited several significant merits, such as excellent selectivity, tolerance to interference and fast binding equilibria. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.034DOI Listing
August 2019
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Miniaturized array gas membrane separation strategy for rapid analysis of complex samples by surface-enhanced Raman scattering.

Anal Chim Acta 2019 Aug 18;1065:29-39. Epub 2019 Mar 18.

School of Chemistry, SunYat-senUniversity, Guangzhou, 510275, China. Electronic address:

It remains a significant challenge for fast and high-throughput detection of trace analytes in complex samples with surface-enhanced Raman scattering (SERS) strategy due to the severe interference from matrices. In this work, a miniaturized array gas membrane separation (AGMS) device coupled with SERS was designed and drew up to eliminate matrix influence and improve the reproducibility of SERS signal during real sample analysis. The design of miniaturized AGMS tube was optimized based on quantitative calculation of its air permeability by computational fluid dynamics simulation. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.031DOI Listing

Facile electrochemiluminescence sensing platform based on water-soluble tungsten oxide quantum dots for ultrasensitive detection of dopamine released by cells.

Anal Chim Acta 2019 Aug 27;1065:21-28. Epub 2019 Mar 27.

Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350108, China; The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350108, China. Electronic address:

This report outlines an ultrasensitive electrochemiluminescence (ECL) sensing platform based on water-soluble tungsten oxide quantum dots (WO QDs) for the detection of dopamine (DA) released from P12 cells. The WO QDs with good stability and water solubility were prepared by a facile and green hydrothermal method, and used to modify a glassy carbon electrode (WO QDs/GCE). The proposed ECL sensor exhibited a stable and strong cathodic ECL signal when potassium peroxodisulfate (KSO) as the coreactant in aqueous solution. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.044DOI Listing

A novel ratiometric and reversible fluorescence probe with a large Stokes shift for Cu based on a new clamp-on unit.

Anal Chim Acta 2019 Aug 18;1065:134-141. Epub 2019 Mar 18.

College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, PR China.

A novel ratiometric and reversible chemosensor 4-((2-(Benzo[d]thiazol-2-yl)phenyl)ethynyl)-N,N-diethylaniline (BT-1) based on ortho-arylethynyl benzothiazole with large Stokes shift (Δλ≈190 nm) was designed and synthesized to recognize Cu. Copper ion induces a remarkable fluorescence enhancement and causes formation of a BT-1-Cu complex. The clamp-on coordination mode of BT-1 to Cu was demonstrated using Job's plot, mass spectrum (MS) and DFT calculations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193031
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http://dx.doi.org/10.1016/j.aca.2019.03.029DOI Listing
August 2019
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Berberine derivatives as heteroatom induced hydrophobic sensor: An analytical approach for the selective and sensitive fluorometric detection and discrimination of serum albumins.

Anal Chim Acta 2019 Aug 15;1065:124-133. Epub 2019 Mar 15.

Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721 102, West Bengal, India. Electronic address:

The detection and discrimination of serum albumins (SAs) has been transforming as a research work of keen interest to the scientists in recent times. This is in the root of foundation of more and more fluorescent probes to selectively identify and distinguish the SAs in the modern era of research. Fluorescence based sensors are preferably on high demand because of high sensitivity of fluorescence spectroscopy. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.026DOI Listing

New bioanalytical platform based on the use of avidin for the successful exfoliation of multi-walled carbon nanotubes and the robust anchoring of biomolecules. Application for hydrogen peroxide biosensing.

Anal Chim Acta 2019 Aug 22;1065:12-20. Epub 2019 Mar 22.

INFIQC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre S/N, Ciudad Universitaria, 5000, Córdoba, Argentina. Electronic address:

We are reporting an innovative building-block for the development of biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with avidin (MWCNTs-avidin). In this work, at variance with previous reports, avidin has the double role of simultaneously being the exfoliating agent of MWCNTs and the platform for anchoring different biotinylated biomolecules. The optimum dispersion was obtained by sonicating for 5. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.022DOI Listing

A highly selective and sensitive dual-mode sensor for colorimetric and turn-on fluorescent detection of cyanide in water, agro-products and living cells.

Anal Chim Acta 2019 Aug 15;1065:113-123. Epub 2019 Mar 15.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People's Republic of China.

A new highly selective and sensitive dual-mode sensor 3TD based on the conjugate of oligothiophene and barbituric acid moiety was developed for colorimetric and turn-on fluorescent detection of CN. The sensor 3TD exhibited high specificity towards CN by interrupting its own ICT producing thereupon a large blue-shift in UV-Vis spectrum and remarkable "turn-on" fluorescence emission. The detection limit was 2. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.024DOI Listing

An ultrasensitive electrochemical sensor for 17β-estradiol using split aptamers.

Anal Chim Acta 2019 Aug 18;1065:107-112. Epub 2019 Mar 18.

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Herein, a simple electrochemical sensor is proposed for 17β-estradiol (E2) determination. In this sensing platform, split DNA aptamers for E2 were applied as recognizing agents. In the presence of E2, split aptamers are bound to E2 and establish split1-E2-split2 complex as a bridge on the surface of electrode. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.062DOI Listing
August 2019
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Microwave-assisted sample preparation of medicines for determination of elemental impurities in compliance with United States Pharmacopeia: How simple can it be?

Anal Chim Acta 2019 Aug 13;1065:1-11. Epub 2019 Mar 13.

Group for Applied Instrumental Analysis, Department of Chemistry, Federal University of São Carlos, P.O. Box 676, São Carlos, SP, 13560-270, Brazil. Electronic address:

This work proposed a procedure for microwave-assisted sample preparation of medicines using diluted nitric acid followed by determination of elemental impurities using inductively coupled plasma optical emission spectrometry (ICP OES) and inductively coupled plasma mass spectrometry (ICP-MS) according to the United States Pharmacopeia Chapters 232 and 233. Three solutions, i.e. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.016DOI Listing

Subtyping of influenza A H1N1 virus using a label-free electrochemical biosensor based on the DNA aptamer targeting the stem region of HA protein.

Anal Chim Acta 2019 Aug 9;1064:94-103. Epub 2019 Mar 9.

Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea; School of Mechanical, Aerospace and Nuclear Engineering, UNIST, Ulsan, 44919, Republic of Korea. Electronic address:

Rapid subtyping of influenza viruses in clinical laboratories has been increasingly important because three subtypes (seasonal H1N1, H3N2, and 2009 H1N1) of influenza A virus currently disseminated in humans have variable susceptibilities to antiviral drug. Herein, we present DNA aptamers for selective detection of influenza A H1N1 (seasonal and 2009 pandemic H1N1) viruses by targeting recombinant influenza A mini-hemagglutinin (mini-HA) protein (the stable stem region of HA) and whole H1N1 viruses. The dissociation constants (K) of aptamer candidates V46 and V57 were 19. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.005DOI Listing

A ratiometric fluorescence probe based on a novel recognition mechanism for monitoring endogenous hypochlorite in living cells.

Anal Chim Acta 2019 Aug 8;1064:87-93. Epub 2019 Mar 8.

Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, 250033, PR China. Electronic address:

A ratiometric fluorescence probe (named ZOC) for the fast detection of HClO/ClO was constructed by coumarin (donor) and pyridinium (acceptor) based on Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. ZOC possessed red emission signal (610 nm), large Stocks shift (190 nm), high energy transfer efficiency (95.3%), high selectivity and sensitivity, low detection limit (25 nM), wider detection range (from 25 nM to 30 μM), rapid response (within 13 S), and good biocompatibility. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.004DOI Listing

Crystalline sponge-laser desorption ionization (CS-LDI) of unsaturated cyclic organic compounds encapsulated in different electronic environments in pores.

Anal Chim Acta 2019 Aug 17;1064:80-86. Epub 2019 Mar 17.

Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa, 769-2193, Japan. Electronic address:

We have developed laser desorption ionization mass spectrometry (LDI-MS) of organic molecules encapsulated in a crystalline sponge (CS). Cyclic organic compounds 1,2,3,4,5-pentamethylcyclopentadiene, zerumbone, and muscone were encapsulated in CS as guest regardless of guest crystallization. Single-crystal X-ray analysis closely scrutinized the position of the guest in the pore and the interaction between the guest and the CS framework. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.011DOI Listing

Integrating a generalized data analysis workflow with the Single-probe mass spectrometry experiment for single cell metabolomics.

Anal Chim Acta 2019 Aug 11;1064:71-79. Epub 2019 Mar 11.

Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK, 73019, USA. Electronic address:

We conducted single cell metabolomics studies of live cancer cells through online single cell mass spectrometry (SCMS) experiments combined with a generalized comprehensive data analysis workflow. The SCMS experiments were carried out using the Single-probe device coupled with a mass spectrometer to measure molecular profiles of cells in response to two mitotic inhibitors, taxol and vinblastine, under a series of treatment conditions. SCMS metabolomic data were analyzed using a comprehensive approach, including data pre-treatment, visualization, statistical analysis, machine learning, and pathway enrichment analysis. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.006DOI Listing

A rapid MS/MS method to assess the deuterium kinetic isotope effect and associated improvement in the metabolic stability of deuterated biological and pharmacological molecules as applied to an imaging agent.

Anal Chim Acta 2019 Aug 22;1064:65-70. Epub 2019 Feb 22.

Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW, 2232, Australia. Electronic address:

The deuterium kinetic isotope effect has been known for a period of 40 years, but it is only relatively recently that new drug entities (NDEs) incorporating deuterium demonstrating beneficial pharmacokinetics, pharmacodynamics, and toxicology have arrived to market. Determination of the precise location to deuterate and subsequently any evaluation for a kinetic isotope effect (KIE) is challenging. Typically, such an evaluation would be performed in an in vitro metabolic assay (e. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.025DOI Listing

A concept for studying the transformation reaction of hexachlorocyclohexanes in food webs using multi-element compound-specific isotope analysis.

Anal Chim Acta 2019 Aug 18;1064:56-64. Epub 2019 Mar 18.

Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany. Electronic address:

An analytical concept using stable isotope fractionation for analyzing persistent organic pollutants (POPs) in food webs was developed and tested. We have evaluated methods for the extraction and clean-up of hexachlorocyclohexane isomers (HCHs) as the model compounds of POPs from water, soil, plant, milk, fish oil and pork liver in order to study the reactive transport processes of HCHs in food webs using multi-element compound-specific isotope analysis (CSIA). The extraction and clean-up methods were evaluated for recovery efficiency and isotope effects. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.030DOI Listing

Green protocol for the preparation of hydrophilic molecularly imprinted resin in water for the efficient selective extraction and determination of plant hormones from bean sprouts.

Anal Chim Acta 2019 Aug 15;1064:47-55. Epub 2019 Mar 15.

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Public Health, Hebei University, Baoding, 071002, China; Key Laboratory of Analytical Science and Technology of Hebei Province & College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China. Electronic address:

In this study, a novel and green synthesis of a new hydrophilic molecularly imprinted 3-aminophenol-hexamethylenetetramine (MIAPH) resin for the selective recognition and separation of plant hormones was developed. The MIAPH resin was obtained using 3-aminophenol as multifunctional monomer which introduced hydroxyl, amino, and imino groups simultaneously, and adenine was used as a dummy template for molecular imprinting. Meanwhile, hexamethylenetetramine released formaldehyde slowly through hydrolysis which was used as the cross-linking agent to avoid the direct and excessive use of toxic formaldehyde. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.025DOI Listing
August 2019
5 Reads

A rapid and sensitive method for the determination of inorganic chloride in oil samples.

Anal Chim Acta 2019 Aug 9;1064:40-46. Epub 2019 Mar 9.

National Research Council Canada, 1200 Montreal Road, K1A 0R6, Ottawa, Ontario, Canada. Electronic address:

A novel isotope dilution method for the analysis of inorganic chloride in fuel oil matrix is presented. The samples were diluted by isopropyl alcohol:toluene, mixed with Cl internal standard and reacted with triethyloxonium tetrafluoroborate at room temperature. This reagent promoted conversion of Cl into stable ethyl chloride (EtCl) which was selectively detected by headspace GC-MS/MS with no matrix effects. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193026
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http://dx.doi.org/10.1016/j.aca.2019.02.055DOI Listing
August 2019
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Sensitive electrochemical biosensor for MicroRNAs based on duplex-specific nuclease-assisted target recycling followed with gold nanoparticles and enzymatic signal amplification.

Anal Chim Acta 2019 Aug 15;1064:33-39. Epub 2019 Mar 15.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, National and Local Joint Engineering Research Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address:

The detection of sequence-specific microRNAs (miRNAs) is an important factor to the diseases diagnosis. Herein, a triple signal amplification electrochemical biosensor for highly sensitive detection of miRNA-21 was developed based on a duplex-specific nuclease (DSN)-assisted target recycling combined with gold nanoparticles (NPs), horseradish peroxidase (HRP) enzymatic signal amplification. The electrochemical biosensor generated significantly amplified amperometric current changes (Δi) for the detection of miRNA-21 down to 43. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.060DOI Listing
August 2019
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Cellular interface supported toehold strand displacement cascade for amplified dual-electrochemical signal and its application for tumor cell analysis.

Anal Chim Acta 2019 Aug 15;1064:25-32. Epub 2019 Mar 15.

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, 200444, PR China. Electronic address:

In this work, toehold strand displacement cascade (TSDC) has been delicately designed and carried out on the cellular interface for the amplification and output of dual-electrochemical signal. Specifically, antibody cross-linked T strand can recognize cell which is linked with immune-magnetic bead. Subsequently, T strand on the cellular interface can mediate the occurrence of TSDC, resulting the change of SN/S1-MB to SN/S2-Fc ratio in the supernatant after magnetic separation. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.021DOI Listing
August 2019
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An atomic fluorescence spectrometer for monitoring nitrogen nutrients via NO vapor generation.

Anal Chim Acta 2019 Aug 8;1064:17-24. Epub 2019 Mar 8.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.

Long-term and multipoint monitoring is very important to evaluate the safety of water environment. Atomic fluorescence spectrometer (AFS) is generally used for analyzing metals or metalloids in water quality monitoring. In the present work, for the first time, AFS is employed for sensitive monitoring of nitrogen nutrients, i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193026
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http://dx.doi.org/10.1016/j.aca.2019.02.057DOI Listing
August 2019
2 Reads

Impact of sample dimensionality on orthogonality metrics in comprehensive two-dimensional separations.

Anal Chim Acta 2019 Aug 12;1064:138-149. Epub 2019 Mar 12.

Laboratory of Metabolomics, Faculty of Medicine and Dentistry, Palacký University Olomouc, Hněvotínská 5, 779 00, Olomouc, Czech Republic; Department of Clinical Chemistry, University Hospital Olomouc, I. P. Pavlova 6, 779 00, Olomouc, Czech Republic. Electronic address:

Orthogonality is a key parameter in the evaluation of the performance of a 2D chromatography-based separation system. Two different perspectives on orthogonality are determined: the extent of the separation space utilized (global orthogonality) and the uniformity of the coverage of the separation space (local orthogonality). This work aims to elucidate the impact of sample dimensionality (the number of separation processes involved) on orthogonality evaluation through the use of descriptors from seven different algorithms utilizing mutually different properties of a chromatogram: Pearson correlation, conditional entropy, asterisk equations, convex hull, arithmetic mean (AN) and harmonic mean of the nearest neighbor, and geometric surface coverage (SC). Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.018DOI Listing

Pore size effect on the separation of polymers by interaction chromatography. A Monte Carlo study.

Anal Chim Acta 2019 Aug 12;1064:126-137. Epub 2019 Mar 12.

Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 00, Praha 2, Czech Republic. Electronic address:

When the polymers are studied by interaction chromatography (IC) in porous media, the IC separation mechanism competes with the size-exclusion chromatography (SEC) mechanism and under specific conditions close to the critical adsorption point (CAP), the elution times of monodisperse polymer samples nonmonotonically depend on pore sizes. We performed Monte Carlo (MC) simulations to elucidate this intriguing effect. By analyzing the behavior of self-avoiding and intersecting chains in two-dimensionally (2D)-confining square pores and in 1D-confining slits in good and Θ-solvents, we confirmed that the dimensionality of the confinement, more specifically, pore geometry, controls the chromatographic behavior. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193029
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http://dx.doi.org/10.1016/j.aca.2019.03.017DOI Listing
August 2019
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Highly sensitive fluorescent metal-organic framework as a selective sensor of Mn and Cr anions (MnO/CrO/CrO) in aqueous solutions.

Anal Chim Acta 2019 Aug 15;1064:119-125. Epub 2019 Mar 15.

Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-4838, Tehran, Iran. Electronic address:

A luminescent Zn-MOF named TMU-41, [Zn(BDC)(L)(DMF)]·1.5DMF, (L = pyridine 4-carboxylic acid and BDC = benzene-1,4-dicarboxylate), has been successfully assembled and well characterized by single-crystal X-ray crystallography, thermogravimetric analysis (TGA), X-ray diffraction (XRD) and N adsorption-desorption isotherms, which shows good stability especially in aqueous solutions. To fabricate open metal sites (OMS) of TMU-41(OMS), the coordinated DMF molecules were exchanged and removed by using acetonitrile as exchanging solvent and drying at 250 °C. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.061DOI Listing
August 2019
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Bioluminescent aptamer-based sandwich-type assay of anti-myelin basic protein autoantibodies associated with multiple sclerosis.

Anal Chim Acta 2019 Aug 12;1064:112-118. Epub 2019 Mar 12.

Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Krasnoyarsk, 660036, Russia.

Bioluminescent solid-phase sandwich-type microassay was developed to detect multiple sclerosis (MS)-associated autoantibodies in human sera. The assay is based on two different 2'-F-Py RNA aptamers against the target autoantibodies as biospecific elements, and Ca-regulated photoprotein obelin as a reporter. The paper describes elaboration of the assay and its application to 91 serum samples from patients with clinically definite MS and 86 ones from individuals healthy in terms of MS. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.015DOI Listing
August 2019
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Accuracy improvement of quantitative analysis for major elements in laser-induced breakdown spectroscopy using single-sample calibration.

Anal Chim Acta 2019 Aug 7;1064:11-16. Epub 2019 Mar 7.

Wuhan National Laboratory for Optoelectronics (WNLO), Huazhong University of Science and Technology, Wuhan, Hubei, 430074, PR China.

Accurate determination of major elements using limited standard samples is always a big challenge in laser-induced breakdown spectroscopy (LIBS). Based on a simple calculation process, we propose a new one-point calibration method called single-sample calibration LIBS (SSC-LIBS) to build the calibration and improve the accuracy of determination of major elements. In this work, several major elements (Fe, Cu, Zn, Ni, Cr, Nb, and Mo) in three sets of matrix-matched certified samples were determined without sample preparation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193026
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http://dx.doi.org/10.1016/j.aca.2019.02.056DOI Listing
August 2019
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Proximity ligation assay induced hairpin to DNAzyme structure switching for entropy-driven amplified detection of thrombin.

Anal Chim Acta 2019 Aug 9;1064:104-111. Epub 2019 Mar 9.

State Key Laboratory of Environment-Friendly Energy Material, Southwest University of Science and Technology, Mianyang, 621010, China.

A proximity ligation assay (PLA) induced hairpin to DNAzyme structure switching strategy has been described for entropy-driven amplified detection of thrombin. The enzyme-strand (E-DNA) and substrate-strand (S-DNA) of DNAzyme are locked in hairpins structure, and the catalytic activity of DNAzyme is inhibited simultaneously. However, in the presence of thrombin, the PLA can induce the unlocking of hairpin, and then the forming of active DNAzyme. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.007DOI Listing
August 2019
1 Read

Selenium-isotopic signature toward mass spectrometric identification and enzyme activity assay.

Anal Chim Acta 2019 Aug 21;1064:1-10. Epub 2019 Mar 21.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, PR China. Electronic address:

The unraveling of enzymatic reactions, especially identification of enzymatic substrates or products, is important to elucidate biological processes. Here a selenium-isotopic signature for mass spectrometric identification of enzymatic-related species is demonstrated by using selenium-containing peptides (SePeps) as substrates. Thus a strategy is proposed for rapid and precise assay of multiple enzyme activity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193034
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http://dx.doi.org/10.1016/j.aca.2019.03.045DOI Listing
August 2019
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An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes.

Anal Chim Acta 2019 Jul 7;1063:99-109. Epub 2019 Feb 7.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address:

The ability of LC-MS/MS for high coverage metabolite analysis lags behind the requirements of global metabolomics. The introduction of chemical derivatizations could significantly extend the ability of LC-MS/MS with enhanced MS response and improved LC separation, which has been serving as a promising quantitative tool for metabolomic analysis. However, as one specific derivatization reagent usually targets to a certain moiety, only a single chemical-group-based submetabolome could be analyzed in one injection. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.001DOI Listing

RNA sampling from tissue sections using infrared laser ablation.

Anal Chim Acta 2019 Jul 7;1063:91-98. Epub 2019 Mar 7.

Department of Chemistry, Louisiana State University, Baton Rouge, LA, 70803, United States. Electronic address:

RNA was obtained from discrete locations of frozen rat brain tissue sections through infrared (IR) laser ablation using a 3-μm wavelength in transmission geometry. The ablated plume was captured in a microcentrifuge tube containing RNAse-free buffer and processed using a commercial RNA purification kit. RNA transfer efficiency and integrity were evaluated based on automated electrophoresis in microfluidic chips. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193026
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http://dx.doi.org/10.1016/j.aca.2019.02.054DOI Listing
July 2019
3 Reads

Minimalist strategies applied to analysis of forensic samples using elemental and molecular analytical techniques - A review.

Anal Chim Acta 2019 Jul 11;1063:9-17. Epub 2019 Feb 11.

National Institute of Science and Technology, INCT for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, 13083-970, Campinas, SP, Brazil; Group of Spectrometry, Sample Preparation and Mechanization - GEPAM, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, 13083-970, Campinas, SP, Brazil. Electronic address:

Forensic science is an emerging field driven by a number of factors, and the development of different methods of analyses, instruments, and techniques is of great help to experts in the field. Sampling and sample preparation in forensic cases are of utmost importance, and therefore, the methods for processing (or not) the samples are critical for acquiring accurate results. Some alternatives for attaining the minimalist concept, i. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.003DOI Listing

Development of a titanium dioxide-assisted preconcentration/on-site vapor-generation chip hyphenated with inductively coupled plasma-mass spectrometry for online determination of mercuric ions in urine samples.

Anal Chim Acta 2019 Jul 4;1063:82-90. Epub 2019 Mar 4.

Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, No. 101, Sec. 2, Kuang-Fu Rd., Hsinchu City, 30013, Taiwan. Electronic address:

In this study, a novel automatic analytical methodology using a titanium dioxide (TiO)-assisted preconcentration/on-site vapor-generation (VG) chip hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) for online determination of mercuric ions (Hg) was developed. Interestingly, the TiO nanoparticle (nano-TiO) coating on the channel surface acted not only as a sorbent for preconcentration but also as a catalyst for photocatalyst-assisted VG. Under optimum operation conditions, the developed method was validated by analyzing the certified reference material (CRM) Seronorm™ Trace Elements Urine L-2 (freeze-dried human urine). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193022
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http://dx.doi.org/10.1016/j.aca.2019.02.035DOI Listing
July 2019
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Rapid untargeted screening for drug residues in animal tissues with liquid microjunction surface sampling probe mass spectrometry.

Anal Chim Acta 2019 Jul 4;1063:75-81. Epub 2019 Feb 4.

Toxicology Program, Iowa State University, 1800 Christensen Drive, Ames, IA, USA; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, USA. Electronic address:

An untargeted screening method for the rapid identification of veterinary drug residues in incurred animal tissues using liquid microjunction surface sampling probe mass spectrometry (LMJSSP-MS) was developed. Current analytical methods for veterinary drug residue screening involve lengthy sample preparation, extraction, and instrumental analysis steps. This method identifies veterinary drug residues in several different incurred animal tissues more quickly than conventional analytical methods. Read More

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http://dx.doi.org/10.1016/j.aca.2019.01.029DOI Listing

Sensitive and anti-interference stripping voltammetry analysis of Pb(II) in water using flower-like MoS/rGO composite with ultra-thin nanosheets.

Anal Chim Acta 2019 Jul 9;1063:64-74. Epub 2019 Mar 9.

Key Lab for High Performance Nonferrous Metals of Anhui Province, Anhui Polytechnic University, Wuhu, Anhui, 241000, PR China; Key Laboratory of Environmental Optics and Technology, And Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, 230031, PR China.

Herein, combined with the outstanding adsorption ability of flower-like MoS with ultra-thin nanosheets and the good electrical conductivity of reduced graphene oxide (rGO), a sensitive and anti-interference electrochemical sensing interface for the analysis of Pb(II) was constructed by using MoS/rGO nanocomposite modified glassy carbon electrode (MoS/rGO-GCE). The stripping behavior of Pb(II) was estimated using square wave anodic stripping voltammetry (SWASV), a high sensitivity of 50.80 μA μM was achieved, and the limit of detection (LOD) was determined to be 0. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.008DOI Listing

Short-chain oligonucleotide detection by glass nanopore using targeting-induced DNA tetrahedron deformation as signal amplifier.

Anal Chim Acta 2019 Jul 11;1063:57-63. Epub 2019 Mar 11.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230026, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Glass capillary nanopore has been developed as a promising sensing platform for bioassay with single-molecule resolution. Although the diameter of glass capillary nanopore can be easily tuned, direct event-readouts of small biomacromolecules, like short-chain oligonucleotide fragments (within ∼20 nucleotides) remain great challenge, which limited by the configuration of the conical-shaped nanopore and the instrumental temporal resolution. Here, we exploit a smart strategy for glass nanopore detection of short-chain oligonucleotides by using relatively big-sized tetrahedral DNA nanostructures as a signal amplifier, which can amplify the signals and retard the translocation speed meanwhile. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.058DOI Listing

A novel strategy for rapidly and accurately screening biomarkers based on ultraperformance liquid chromatography-mass spectrometry metabolomics data.

Anal Chim Acta 2019 Jul 12;1063:47-56. Epub 2019 Mar 12.

The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, PR China. Electronic address:

We reported a novel strategy for rapidly and accurately screening biomarkers based on ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) metabolomics data. First, the preliminary variables were obtained by screening the original variables using method validation. Second, the variables were selected from the preliminary variables and formed the variable sets by testing different thresholds of single factor (variable importance in projection (VIP), fold change (FC), the area under the receiver operating characteristic curve (AUROC), and -ln(p-value)). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00032670193028
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http://dx.doi.org/10.1016/j.aca.2019.03.012DOI Listing
July 2019
3 Reads

Uncertainty estimation and misclassification probability for classification models based on discriminant analysis and support vector machines.

Anal Chim Acta 2019 Jul 15;1063:40-46. Epub 2018 Sep 15.

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, United Kingdom.

Uncertainty estimation provides a quantitative value of the predictive performance of a classification model based on its misclassification probability. Low misclassification probabilities are associated with a low degree of uncertainty, indicating high trustworthiness; while high misclassification probabilities are associated with a high degree of uncertainty, indicating a high susceptibility to generate incorrect classification. Herein, misclassification probability estimations based on uncertainty estimation by bootstrap were developed for classification models using discriminant analysis [linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA)] and support vector machines (SVM). Read More

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http://dx.doi.org/10.1016/j.aca.2018.09.022DOI Listing

Staring at protein-surfactant interactions: Fundamental approaches and comparative evaluation of their combinations - A review.

Anal Chim Acta 2019 Jul 22;1063:18-39. Epub 2019 Feb 22.

Materials Science and Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea. Electronic address:

Protein-surfactant interactions have been explored for decades owing to their widespread application in the pharmaceutical, food, and cosmetics industries and their importance to biochemical systems. However, they require further study owing to their compositional complexity and the innate limitations of current analytical approaches. In this review, we briefly introduce a series of individual approaches used for the qualitative and quantitative investigation of protein-surfactant interactions, including absorbance- or emission-based spectroscopy, scattering-based spectroscopy, mass spectrometry, calorimetry, computation and microscopy. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.024DOI Listing
July 2019
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Isoelectric focusing array with immobilized pH gradient and dynamic scanning imaging for diabetes diagnosis.

Anal Chim Acta 2019 Jul 12;1063:178-186. Epub 2019 Mar 12.

Department of Instrument Science and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China; School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

A traditional immobilized pH gradient (IPG) has a high stability for isoelectric focusing (IEF) but suffers from time-consuming rehydration, focusing and staining-imaging as well as complex performance. To address these issues, an IEF system with an array of 24 IPG columns (10 mm × 600 μm × 50 μm) and dynamic scanning imaging (DSI) was firstly designed for protein focusing. Moreover, two IPG columns (pH 4-9 and pH 6. Read More

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http://dx.doi.org/10.1016/j.aca.2019.03.014DOI Listing
July 2019
3 Reads

Sub-1 mL sample requirement for simultaneous determination of 17 organic and inorganic anions and cations in Antarctic ice core samples by dual capillary ion chromatography.

Anal Chim Acta 2019 Jul 19;1063:167-177. Epub 2019 Feb 19.

Australian Centre for Research on Separation Science, School of Natural Sciences, University of Tasmania, GPO Box 252-75, Hobart, Tasmania, 7001, Australia.

The significant advance of delivering high value multi-species data from sub-1 mL ice core sample volumes allows higher temporal resolution in deposition records of inorganic and low molecular weight organic anions and cations. The determination of these species is a fundamental strategic requirement in modern paleoclimate studies. Herein, for the first time, a dual capillary ion chromatography (Cap-IC) based method for the simultaneous separation of 17 organic and inorganic anions and cations in low volume Antarctic ice core samples is presented. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.014DOI Listing

Single drop microextraction in a 96-well plate format: A step toward automated and high-throughput analysis.

Anal Chim Acta 2019 Jul 21;1063:159-166. Epub 2019 Feb 21.

Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil. Electronic address:

In this study, an innovative and high-throughput parallel-single-drop microextraction (Pa-SDME) using the [P][MnCl] magnetic ionic liquid (MIL) as extraction phase is demonstrated, for the first time, in the determination of methylparaben, ethylparaben, propylparaben, bisphenol A, butylparaben, benzophenone and triclocarban from environmental aqueous samples. This experimental setup comprised of a 96-well plate system containing a set of magnetic pins which aided in stabilizing the MIL drops and enabled the simultaneous extraction of up to 96 samples. Using this low-cost experimental apparatus, the sample throughput was lower than 1 min per sample. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.013DOI Listing

MnO nanosheet-mediated ratiometric fluorescence biosensor for MicroRNA detection and imaging in living cells.

Anal Chim Acta 2019 Jul 4;1063:152-158. Epub 2019 Mar 4.

School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, PR China. Electronic address:

MicroRNA (miRNA) plays significant roles in cell proliferation, differentiation and apoptosis, and has been considered to be valuable biomarker for cancer. Accurate and sensitive detection of miRNA is crucially significant for cancer diagnosis and treatment. Here, a MnO nanosheet-mediated ratiometric fluorescence biosensor was designed for miRNA detection and imaging in living cells. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.049DOI Listing
July 2019
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Carbon dots synthesized at room temperature for detection of tetracycline hydrochloride.

Anal Chim Acta 2019 Jul 2;1063:144-151. Epub 2019 Mar 2.

Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China. Electronic address:

The traditional synthesis methods of carbon dots (CDs) have some disadvantages of complicated operation and a large amount of energy consumption. To address these limitations, we synthesized yellow-green luminescent CDs at room temperature according to the principle of amine-aldehyde condensation in this work. This reaction is simple, economical, energy saving and is extremely consistent with the concept of green synthesis and sustainable development. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.047DOI Listing

Detection of chloramphenicol in meat with a chemiluminescence resonance energy transfer platform based on molecularly imprinted graphene.

Anal Chim Acta 2019 Jul 3;1063:136-143. Epub 2019 Mar 3.

College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 071000, China. Electronic address:

In this study, a novel composite was synthesized by polymerizing the dummy-template molecularly imprinted microspheres on the surface of magnetic graphene. This composite was used as recognition reagent and energy acceptor to develop a platform for determination of chloramphenicol according to the principle of chemiluminescence resonance energy transfer. The light signal was induced with luminolHO4-(imidazole-1-yl)phenol system, and the chemiluminescence intensity was positively correlated with the analyte concentration. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.044DOI Listing