14,764 results match your criteria Analyst[Journal]


Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications.

Analyst 2022 Jun 24. Epub 2022 Jun 24.

International Joint Research Laboratory of New Energy Materials and Devices of Henan Province, School of Physics and Electronics, Henan University, Kaifeng, 475004, PR China.

Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase. Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction (PCR) amplification, RCA is more suitable for on-spot detection without the need for an expensive thermal cycler. In this study, the principle and classification of RCA are introduced, and the applications of RCA in the detection of pathogenic bacteria, nucleic acid tumor markers, viruses, and proteins are reviewed. Read More

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Ion-selective membrane plasticizer leakage in all-solid-state electrodes - an unobvious way to improve potential reading stability in time.

Analyst 2022 Jun 22. Epub 2022 Jun 22.

Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

The effect of leakage of the plasticizer from the ion-selective membrane into the ion-to-electron transducer of all-solid-state potentiometric sensors is considered for the first time. The plasticizer can be transferred to the transducer phase, either during ion-selective membrane application or later; in both cases, its presence can affect the performance of the sensors. Clearly, this effect is most pronounced if the transducer is dispersible in the plasticizer. Read More

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Applications of upconversion nanoparticles in analytical and biomedical sciences: a review.

Analyst 2022 Jun 22. Epub 2022 Jun 22.

Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat - 395007, Gujarat, India.

Lanthanide-doped upconversion nanoparticles (UCNPs) have gained more attention from researchers due to their unique properties of photon conversion from an excitation/incident wavelength to a more suitable emission wavelength at a designated site, thus improving the scope in the life sciences field. Due to their fascinating and unique optical properties, UCNPs offer attractive opportunities in theranostics for early diagnostics and treatment of deadly diseases such as cancer. Also, several efforts have been made on emerging approaches for the fabrication and surface functionalization of luminescent UCNPs in optical biosensing applications using various infrared excitation wavelengths. Read More

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Smartphone clip-on instrument and microfluidic processor for rapid sample-to-answer detection of Zika virus in whole blood using spatial RT-LAMP.

Analyst 2022 Jun 21. Epub 2022 Jun 21.

Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Rapid, simple, inexpensive, accurate, and sensitive point-of-care (POC) detection of viral pathogens in bodily fluids is a vital component of controlling the spread of infectious diseases. The predominant laboratory-based methods for sample processing and nucleic acid detection face limitations that prevent them from gaining wide adoption for POC applications in low-resource settings and self-testing scenarios. Here, we report the design and characterization of an integrated system for rapid sample-to-answer detection of a viral pathogen in a droplet of whole blood comprised of a 2-stage microfluidic cartridge for sample processing and nucleic acid amplification, and a clip-on detection instrument that interfaces with the image sensor of a smartphone. Read More

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Polydopamine-based molecularly imprinted electrochemical sensor for the highly selective determination of ecstasy components.

Analyst 2022 Jun 21. Epub 2022 Jun 21.

Guangzhou Key Laboratory of Sensing Materials & Devices, Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, c/o School of Civil Engineering, Guangzhou University, Guangzhou 510006, PR China.

3,4-Methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) are the main components of illicit stimulant drugs, also known as "ecstasy", which belong to psychoactive medicine and tend to be increasingly abused among drug addicts worldwide. Herein, an electrochemical sensor based on molecularly imprinted polydopamine ([email protected]) was developed to detect MDA and MDMA using differential pulse voltammetry (DPV). An MIP film on a Au electrode was synthesized electrochemical polymerization with the safe chemical DA as the polymerization monomer and the uncontrolled pharmaceutical intermediate 3,4-methylenedioxyphenethylamine (MDPEA) as the template molecule, which can provide a great quantity of specific binding sites and expand the practical application of the sensor. Read More

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Portable and sensitive detection of cancer cells a handheld luminometer.

Analyst 2022 Jun 21. Epub 2022 Jun 21.

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.

Portable detection of cancer cells is important for early-stage diagnostic applications and prognosis of cancer. Herein, a simple and sensitive chemiluminescence method was proposed for portable detection of cancer cells a handheld luminometer. It is based on the cancer cell triggered cyclic strand displacement reaction (SDR) for signal amplification. Read More

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Smartphone-based photochemical sensor for multiplex determination of glucose, uric acid, and total cholesterol in fingertip blood.

Analyst 2022 Jun 20. Epub 2022 Jun 20.

School of Sensing Science and Engineering, Shanghai Jiao Tong University, Shanghai, China.

A smartphone-based photochemical biosensor capable of realizing multiplex detection of glucose, uric acid (UA), and total cholesterol (TC) is reported here for the first time. The system was composed of a photochemical dongle, a cloud-enabled smartphone, and disposable test strips. The dongle was controlled using a microcontroller unit and was designed to convert the reflected light's intensities into optoelectronic signals for analytes quantification. Read More

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Amine additives for improved in-ESI H/D exchange.

Analyst 2022 Jun 17. Epub 2022 Jun 17.

Center for Computational and Data-Intensive Science and Engineering, Skolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, Russia.

In-ESI H/D exchange is a convenient technique for analyzing small-molecular complex mixtures. However, such experiments do not yield sufficient levels of exchange or require an elevated temperature of the ion transfer capillary. Increased temperature may result in unexpected additional exchanges of -CH groups that may complicate the interpretation of the H/D exchange data used for identification. Read More

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Use of MALDI-TOF mass spectrometry for virus identification: a review.

Analyst 2022 Jun 17. Epub 2022 Jun 17.

Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.

The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) directly or indirectly affects every individual worldwide. The fight against SARS-CoV-2 is based on the rapid and accurate diagnosis and subsequent isolation of infected individuals. Therefore, the demands for the scientific development of diagnostic methods for the confirmation of SARS-CoV-2 are enormous. Read More

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Design of a photoelectrochemical lab-on-a-chip immunosensor based on enzymatic production of quantum dots .

Analyst 2022 Jun 17. Epub 2022 Jun 17.

Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 182, 20014, Donostia San Sebastián, Spain.

In this work we report the development and validation of a photoelectrochemical immunosensor on the basis of alkaline phosphatase (ALP)-linked immunoassay for the detection of human serum albumin as a model analyte. In this biosensor, oriented immobilization of capture antibodies on aminated polystyrene was achieved physical adsorption. After the interaction with the analyte, ALP immobilised on the surface through the sandwich immunoassay catalyses the hydrolysis of sodium thiophosphate (TP) to hydrogen sulphide (HS) which in the presence of cadmium ions yields CdS quantum dots (QDs). Read More

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lipid profiling of insect pheromone glands by direct analysis in real time mass spectrometry.

Analyst 2022 Jun 17. Epub 2022 Jun 17.

Pacific Biosciences Research Center, School of Environment and Ocean Science Technology, University of Hawai'i at Mānoa, 1993 East West Road, Honolulu, HI 96822, USA.

Lipid pheromones play a significant role in the behavior and ecology of many insects. The characterization of pheromone structures is a significant challenge due to their low abundance and ephemeral nature. Here we present a method for the analysis of lipid molecules from single pheromone glands of (fruit fly) using Direct Analysis in Real Time mass spectrometry (DART MS). Read More

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A direct mass spectrometry method for cannabinoid quantitation in urine and oral fluid utilizing reactive paper spray ionization.

Analyst 2022 Jun 27;147(13):3109-3117. Epub 2022 Jun 27.

Applied Environmental Research Laboratories, Department of Chemistry, Vancouver Island University, Nanaimo, BC, Canada.

A direct mass spectrometry method utilizing reactive paper spray ionization was developed for sensitive cannabinoid quantitation in biofluid matrices. The 2-minute sample measurements used on-paper derivatization to significantly increase paper spray mass spectrometry (PS-MS) positive ion mode sensitivity while minimizing sample preparation steps. Calibrations demonstrate high linearity, with > 0. Read More

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Trihexyltetradecylphosphonium chloride based ratiometric fluorescent nanosensors for multiplex anion discrimination.

Analyst 2022 Jun 16. Epub 2022 Jun 16.

State Key Laboratory of Analytical Chemistry for Life science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P.R. China.

Compared with the well-studied cations, the development of methods for anion detection is relatively slow due to the anion characteristics such as a complex geometry, strong hydration and a low charge density. Herein, a colorimetric and ratiometric fluorescent anion sensing platform based on trihexyltetradecylphosphonium chloride ([THTP][Cl]) was developed for the first time. Such nanosensors exhibited a pH response of 5-7 as well as a high selectivity to perchlorate. Read More

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Mansfieldite (AlAsO·2HO): a new white pigment in Himalayan artwork.

Analyst 2022 Jun 15. Epub 2022 Jun 15.

Indiana University-Purdue University Indianapolis, Department of Earth Sciences, 723 W. Michigan Street, Indianapolis, IN 46220, USA.

An unusual arsenate mineral, mansfieldite (AlAsO·2HO), was identified as a pigment for the first time as the principal white colorant on two Himalayan thangka paintings at the Indianapolis Museum of Art at Newfields. The co-occurrence of this unusual mineral pigment provides support for the belief that the two artworks are members of a cycle of paintings originating from the same workshop, perhaps from Chamdo, Tibet. The complete palettes of both artworks are identical, including the use of mansfieldite, brochantite, malachite, azurite, vermilion, gold, orpiment, and a carbon-based black in a glue binder on a calcite and gypsum-primed cotton fabric. Read More

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Imaging mass spectrometry differentiates the effects of doxorubicin formulations on non-targeted tissues.

Analyst 2022 Jun 14. Epub 2022 Jun 14.

MyBiotech, Überherrn, Germany.

Administration of cytotoxic agents like doxorubicin (DOX) is restrained by the effects on different non-targeted/non-cancerous tissues, which instigates the development of nano-enabled drug delivery systems, among others. In this study, imaging mass spectrometry (IMS) was selected to examine the effects of DOX nanoformulations on non-targeted tissues. Chemical alterations induced by liposomal (LPS) and poly (lactic--glycolic acid) (PLG) nanoformulations were assessed against the ones induced by the conventional (CNV) formulation. Read More

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A highly selective and sensitive CdS fluorescent quantum dot for the simultaneous detection of multiple pesticides.

Analyst 2022 Jun 14. Epub 2022 Jun 14.

College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

The abuse of pesticides has introduced a large number of residues in soil and drinking water, which can then enter the food chain and the human body. Monitoring pesticide residues and developing simple and fast detection systems for pesticide residues is urgently needed. In this study, we presented one-pot prepared CdS fluorescent quantum dots (QDs) and explored their sensing application for organic pesticides. Read More

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A functionalized magnetic nanoparticle regulated CRISPR-Cas12a sensor for the ultrasensitive detection of alpha-fetoprotein.

Analyst 2022 Jun 14. Epub 2022 Jun 14.

Department of Clinical Laboratory, Shanghai Tenth People's Hospital Chong Ming Branch, 202150, China.

Alpha-fetoprotein (AFP) is an important clinical tumor marker of hepatoblastoma, and the concentration of AFP in serum is closely related to the staging of hepatoblastoma. We report a magnetic bead separation platform based on a switching aptamer triggered hybridization chain reaction (SAT-HCR) and the CRISPR-Cas12a sensor for the detection of AFP. AFP aptamer, as an easily regulated nucleic acid strand, is responsible for binding to AFP into nucleic acid detection, while HCR-CRISPR-Cas12a, regulated by functionalized magnetic nanoparticles, is responsible for highly specific nucleic acid signal amplification. Read More

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Rapid quantification of microRNA-375 through one-pot primer-generating rolling circle amplification.

Analyst 2022 Jun 27;147(13):2936-2941. Epub 2022 Jun 27.

Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, USA.

A recent surge of interest in microRNA has been driven by its discovery as a circulating biomarker of disease, with many diagnostic test platforms currently under development. Alternatives to widely used microRNA quantification methods such as quantitative reverse transcriptase PCR (qRT-PCR) are needed for use in portable and point-of-care devices which are incompatible with complex sample processing workflows and thermal cycling. Rolling circle amplification (RCA) is a one-pot assay technique which directly amplifies nucleic acids using sequence-specific microRNA priming to initiate a single-step isothermal reaction that is compatible with simple devices. Read More

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Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry.

Analyst 2022 Jun 27;147(13):3101-3108. Epub 2022 Jun 27.

Department of Chemistry, University of Florida, Gainesville, FL 32611, USA.

LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily separated and identified using liquid chromatography (LC)-MS. In addition, like gangliosides, homologous lipid forms of GalNAc-LcGg4 showed the same fragmentation pattern, except for a uniform shift of their glycolipid product ions by a certain / number determined by the varied lipid structure. Read More

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Gold nanoparticles-oxidized multi-walled carbon nanotubes as electrochemiluminescence immunosensors.

Analyst 2022 Jun 27;147(13):3096-3100. Epub 2022 Jun 27.

Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, and College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, P. R China.

Oxidized multi-walled carbon nanotube/nano-gold (AuNP-ox-MWCNT) composites with strong electrochemiluminescence (ECL) activity were applied to construct a new ECL immunosensor for the detection of carcinoembryonic antigen (CEA). The immunosensor showed a linear response range of 10-100 ng mL and detection limit of 0.76 ng mL (at a signal-to-noise ratio of 3). Read More

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Solid-state nanopore analysis on the conformation change of DNA polymerase I induced by a DNA substrate.

Analyst 2022 Jun 27;147(13):3087-3095. Epub 2022 Jun 27.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Proteins with a changeable conformation, such as polymerases, play a very important role in various life activities. Their conformational changes can be reflected in their structural size and flexibility, which may influence their transport kinetics. Recently, solid-state nanopore sensors have been widely applied to characterize the conformation of proteins and other complex structures as sensitive and high throughput single-molecule detectors. Read More

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Recent progress in electrochemiluminescence microscopy analysis of single cells.

Analyst 2022 Jun 27;147(13):2884-2894. Epub 2022 Jun 27.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, China.

Electrochemiluminescence (ECL) is produced by chemiluminescence reactions initiated by electrochemical methods. On this basis, ECL microscopy (ECLM) is an emerging visualization tool to directly study local electrochemical features with good spatial and temporal resolution. Recent progress in ECLM demonstrates that it is particularly important in single-cell studies on the morphological structures and functional characteristics of cells due to the unique heterogeneity of cells. Read More

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A lipid droplet-specific fluorescence probe for atherosclerotic plaque imaging.

Analyst 2022 Jun 27;147(13):3081-3086. Epub 2022 Jun 27.

Laboratory of Heart Valve Disease, West China Hospital, Sichuan University, 37 Guoxue Road, Chengdu 610041, PR China.

The dysregulation of lipid droplets (LDs) is closely related to certain metabolic diseases, while the role of LDs during pathological processes remains mysterious. It would be of great value to monitor the dynamic changes of LDs in a visible way so as to study their biological functions. In this study, we report a LD-specific fluorescence probe TBI for precise LD-targeting imaging in cells and atherosclerotic tissues. Read More

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Correction: Microfluidic ELISA employing an enzyme substrate and product species with similar detection properties.

Analyst 2022 Jun 27;147(13):3118. Epub 2022 Jun 27.

Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA.

Correction for 'Microfluidic ELISA employing an enzyme substrate and product species with similar detection properties' by Basant Giri , , 2018, , 989-998, https://doi.org/10.1039/C7AN01671A. Read More

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Rapid analysis of differential chemical compositions of using thin-layer chromatography spray ionization-mass spectrometry.

Analyst 2022 Jun 27;147(13):3072-3080. Epub 2022 Jun 27.

The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

, with medicinal and edible properties, contains multiple chemical components that pose difficulties in its quality control. Herein, we established a simple thin-layer chromatography spray ionization-mass spectrometry (TLCSI-MS) device that allows the simultaneous separation and identification of bioactive compounds. The triterpene acids in different medicinal parts of were characterized, and the markers of differences were revealed. Read More

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A rare monoclonal antibody discovery based on indirect competitive screening of a single hapten-specific rabbit antibody secreting cell.

Analyst 2022 Jun 27;147(13):2942-2952. Epub 2022 Jun 27.

College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.

A rare antibody that is able to tolerate physio-chemical factors is preferred and highly demanded in diagnosis and therapy. Rabbit monoclonal antibodies (RmAbs) are distinguished owing to their high affinity and stability. However, the efficiency and availability of traditional methods for RmAb discovery are limited, particularly for small molecules. Read More

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Separation of intact proteins by capillary electrophoresis.

Analyst 2022 Jun 27;147(13):2988-2996. Epub 2022 Jun 27.

The Atomic Medicine Initiative, University of Technology Sydney, Sydney, NSW, Australia.

This work introduces novel and universal workflows for the analysis of intact proteins by capillary electrophoresis and presents guidelines for the targeted selection of appropriate background electrolytes (BGEs) by consideration of the target proteins' isoelectric point (pI). The suitability of neutral dimethyl polysiloxane (PDMS) capillaries with dynamic coatings of cationic cetyltrimethylammonium bromide (CTAB) or anionic sodium dodecyl sulfate (SDS), and bare fused silica (BFS) capillaries were systematically evaluated for the analysis of histidine and seven model proteins in six BGEs with pH values between 3.0 and 9. Read More

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An activatable near-infrared hemicyanine-based probe for selective detection and imaging of Hg in living cells and animals.

Analyst 2022 Jun 27;147(13):3065-3071. Epub 2022 Jun 27.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.

Fluorescence imaging in the near-infrared (NIR) window has great potential for clinical diagnosis and treatment because of its deep penetration and high contrast. Here, a NIR hemicyanine-based probe (CyP) was synthesized for selective detection and imaging of Hg in living cells and animals. Using the rigid xanthene structure as the electron donor, trimethylindolenine as the electron acceptor and diphenylphosphinothioic chloride as the recognition element, the probe CyP could specifically respond to Hg and transform into an activated donor-π-acceptor (D-π-A) motif with a NIR emission maximum at 710 nm. Read More

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A novel approach for discovering correlations between elemental and molecular composition using laser-based spectroscopic techniques.

Analyst 2022 Jun 7. Epub 2022 Jun 7.

Department of Chemistry, Lomonosov Moscow State University, Moscow 119234, Russia.

Modern analytical techniques, including laser-induced breakdown spectroscopy (LIBS) and Raman spectroscopy, yield multidimensional data, which are most efficiently used in conjunction with chemometric techniques, including multi-block algorithms. In this study, we use several algorithms for the processing of laser-induced breakdown and Raman spectra of zooplankton organisms, which are found to accumulate lithium for an unknown reason. Correlations between elemental and molecular composition of zooplankton have been found. Read More

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A cascade Fermat spiral microfluidic mixer chip for accurate detection and logic discrimination of cancer cells.

Analyst 2022 Jun 7. Epub 2022 Jun 7.

School of Mechanical Engineering, Yangzhou University, Yangzhou 225127, China.

Since cancer has emerged as one of the most serious threats to human health, the highly sensitive determination of cancer cells is of significant importance to improve the accuracy of early clinical diagnosis. In our investigation, a novel cascade Fermat spiral microfluidic mixer chip (CFSMMC) combined with fluorescence sensors as a point-of-care (POC) testing system is successfully fabricated to detect and differentiate cancer cells (MCF-7) from normal cells with excellent sensitivity and selectivity. Here, copper ions (Cu) with peroxidase properties can catalyze the oxidation of the non-fluorescent substrate Amplex Red (AR) to the highly fluorescent resorufin (ox-AR) in the presence of hydrogen peroxide (HO). Read More

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