12,311 results match your criteria Analyst[Journal]


A visible-light-driven photoelectrochemical molecularly imprinted sensor based on titanium dioxide nanotube arrays loaded with silver iodide nanoparticles for the sensitive detection of benzoyl peroxide.

Analyst 2019 Apr 17. Epub 2019 Apr 17.

Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.

A novel ultrasensitive photoelectrochemical sensor for benzoyl peroxide (BPO) was constructed under visible light irradiation. A novel nanostructured material made of molecularly imprinted polymer (MIP)-modified silver iodide nanoparticle-titanium dioxide nanotube arrays (AgINPs-TiO2 NTs) was designed as a photoactive electrode (denoted as MIP@AgINPs-TiO2 NTs). AgI-sensitized TiO2 nanotube arrays were prepared by a simple dissolution-precipitation-calcination process and then employed as a matrix to graft the MIP recognition element. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00234kDOI Listing

Analytical performance of μ-groove silicon attenuated total reflection waveguides.

Analyst 2019 Apr 17. Epub 2019 Apr 17.

Insitute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

The analytical performance of micromachined μ-groove silicon attenuated total reflection (ATR) elements has been evaluated in a comparison of Fourier-transform infrared (FTIR) and quantum cascade laser (QCL) spectroscopy operating at mid-infrared (MIR) wavelengths. μ-Groove silicon ATR elements are highly efficient micromachined waveguides fabricated at a wafer scale at such low cost that they may be considered a consumable for single-time-use, e.g. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00417cDOI Listing

A new method using machine learning for automated image analysis applied to chip-based digital assays.

Analyst 2019 Apr 16. Epub 2019 Apr 16.

Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China. and College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, P.R. China.

Chip-based digital assays such as the digital polymerase chain reaction (digital PCR), digital loop-mediated amplification (digital LAMP), digital enzyme-linked immunosorbent assay (digital ELISA) and digital proximity ligation assay (digital PLA) need high-throughput quantification of the captured fluorescence image data. However, traditional methods that are mainly based on image segmentation using either a fixed threshold or an automated hard threshold failed to extract valid signals over a broad range of image characteristics. In this study, we introduce a new method for automated image analysis to extract signals applied to chip-based digital assays. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00149B
Publisher Site
http://dx.doi.org/10.1039/c9an00149bDOI Listing
April 2019
1 Read

Primer remodeling amplification-activated multisite-catalytic hairpin assembly enabling the concurrent formation of Y-shaped DNA nanotorches for the fluorescence assay of ochratoxin A.

Analyst 2019 Apr 16. Epub 2019 Apr 16.

School of Biological Sciences and Technology, University of Jinan, Jinan 250022, P.R. China. and Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

DNA can be configured into unique high-order structures due to its significantly high programmability, such as a three-way junction-based structure (denoted Y-shaped DNA), for further applications. Herein, we report a label-free fluorescent signal-on biosensor based on the target-driven primer remodeling rolling circle amplification (RCA)-activated multisite-catalytic hairpin assembly (CHA) enabling the concurrent formation of Y-shaped DNA nanotorches (Y-DNTs) for ultrasensitive detection of ochratoxin A (OTA). Two kinds of masterfully-designed probes, termed Complex I and II, were pre-prepared by the combination of a circular template (CT) with an OTA aptamer (S1), a substrate probe (S2) and hairpin probe 1 (HP1), respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00316aDOI Listing

Affinity separation and subsequent terminal differentiation of acute myeloid leukemia cells using the human transferrin receptor (CD71) as a capture target.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.

The microfluidic detection of myeloblasts in blood via the human transferrin receptor (CD71) can serve as a diagnostic marker for acute myeloid leukemia (AML). Furthermore, CD71 expression is present in all proliferating cells and can capture target cells without prior knowledge of AML subtype. The use of anti-CD71 as the affinity ligand for AML detection in this work yields a capture efficiency and purity during peak CD71 expression of 92% and 62%, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02357cDOI Listing

Rapid detection of metal impurities on the surfaces of intact objects with irregular shapes using electrochemical mass spectrometry.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang 330013, P. R. China.

New approaches are demanded in daily life and industry for the rapid inspection of chemical impurities on various objects, particularly those with irregular shapes. Herein, an analytical strategy combining electrochemistry (EC) and mass spectrometry (MS) has been developed for the direct inspection of metal impurities on various objects which are commonly used in daily life and industry. An intact object (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02472cDOI Listing
April 2019
1 Read

Reaction-based fluorometric analysis of N-bromosuccinimide by oxidative deprotection of dithiane.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemistry, Chung-Ang University, Seoul 06974, Republic of Korea.

In this study, a fluorescent probe is developed for the first time for N-bromosuccinimide (NBS), a synthetically and analytically important compound. Pyrene-dithiane-based probe 1 showed prominently selective and sensitive signaling behavior toward NBS owing to the oxidative cleavage of the dithiane protecting group of 1-pyrenecarboxaldehyde. The NBS-selective signaling of the probe was possible under competitive conditions in the presence of common metal ions and anions as a background. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C8AN02125B
Publisher Site
http://dx.doi.org/10.1039/c8an02125bDOI Listing
April 2019
1 Read

T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

Department of Bioactive Material Sciences, Chonbuk National University, South Korea and Department of Chemistry, Chonbuk National University, South Korea.

A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity-the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00303gDOI Listing

A red mitochondria-targeted AIEgen for visualizing HS in living cells and tumours.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

State Key Laboratory of Chemical Resource Engineering, College of Science, Beijing Advanced Innovation Centre for Soft Matter Science and Engineering, Beijing University of Chemical Technology, North Third Ring Road 15, Chaoyang District, Beijing, 100029, China.

Hydrogen sulfide (H2S) exists in the cytosol and mitochondria of mammalian cells as a signaling molecule. Scientists have explored many important physiological functions of H2S, such as regulating vasodilator relaxation, protecting living cells and avoiding damage. The measurement of H2S is therefore necessary for exploring the biological function of cells and tissues. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00393B
Publisher Site
http://dx.doi.org/10.1039/c9an00393bDOI Listing
April 2019
1 Read

A hybrid model combining wavelet transform and recursive feature elimination for running state evaluation of heat-resistant steel using laser-induced breakdown spectroscopy.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

School of Electric Power, South China University of Technology, Guangzhou, Guangdong 510640, China. and Guangdong Province Engineering Research Center of High Efficiency and Low Pollution Energy Conversion, Guangzhou, Guangdong 510640, China.

Heat-resistant steel is widely used in various industries, and the running state is of great importance for equipment function and safety. In this work, laser-induced breakdown spectroscopy (LIBS) is applied to evaluate the running state of steel using indicators of micro and macro properties. The hybrid model based on wavelet threshold denoising (WTD) and K-fold-support vector machine-recursive feature elimination (K-SVM-RFE) is proposed to estimate the different indictors of various service conditions of steel. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00370cDOI Listing

A novel turn-on fluorescent probe for selective sensing and imaging of glutathione in live cells and organisms.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

A State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, China.

We synthesized six 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD)-based probes with various leaving groups using an arylthioether linker and for the first time identified the probe O-NH2 capable of highly selective detection of glutathione over cysteine/homocysteine in vitro and in vivo based on an aromatic nuclear substitution reaction (SNAr) mechanism. The fluorescence of the probe O-NH2 was quenched because of the photoinduced electron transfer (PET) process, but switched on by a glutathione-triggered specific recognition reaction between the probe O-NH2 and glutathione. The recognition mechanism for glutathione was explored and verified by theoretical calculations and ESI-MS analysis. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00115H
Publisher Site
http://dx.doi.org/10.1039/c9an00115hDOI Listing
April 2019
1 Read

Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification.

Analyst 2019 Apr 15. Epub 2019 Apr 15.

Department of Chemical and Biomolecular Engineering (BK 21+ program), KAIST, Daehak-ro 291, Yuseong-gu, Daejeon 34141, Republic of Korea.

We herein devise a simple and label-free strategy to determine S1 nuclease activity by exploiting the target-induced inhibition of exponential strand displacement amplification (eSDA). In principle, a DNA probe that is designed to produce a large amount of duplexes through a process of eSDA, is degraded by the catalytic activity of S1 nuclease. This reaction blocks the initiation of eSDA, leading to the quite-reduced fluorescence of a double-stranded DNA specific fluorescent dye, SYBR Green I compared to the one in the absence of S1 nuclease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00300bDOI Listing

Fluorescence enhancement novel green analytical method for paraquat herbicide quantification based on immobilization on clay.

Analyst 2019 Apr 12. Epub 2019 Apr 12.

INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, 8000 Bahía Blanca, Argentina.

A new green method was developed for the quantification of paraquat (PQ) in water samples based on the fluorescence emission enhancement of the herbicide signal after adsorption on sodium montmorillonite clay (MMT). Radiant emission processes are favored by increasing the molecular rigidity of the PQ since it adopts a planar position between the nano-sheets of the MMT. The advantages of the use of this clay are nontoxic, low cost and found in abundance in natural reserves. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00387hDOI Listing

Enhancing the colorimetric detection of HO and ascorbic acid on polypyrrole coated fluconazole-functionalized POMOFs.

Analyst 2019 Apr 12. Epub 2019 Apr 12.

Department of Chemistry and Chemical Engineering, Jining University, Qufu 273155, P. R. China.

A new fluconazole-functionalized polyoxometalate-based metal-organic framework (POMOF) [Ag3(FKZ)2(H2O)2][H3SiW12O40] (AgFKZSiW12) was successfully constructed, and its polypyrrole (PPy) coated composite AgFKZSiW12@PPy was also obtained via a facile 'in situ' oxidation polymerization process. The peroxidase-like activity evaluation indicates that the maximized synergistic effect from the integration of PPy, SiW12 clusters, HFKZ drug molecules, and Ag ions deeply enhanced the overall performance. More importantly, AgFKZSiW12@PPy exhibits the fastest response time (30 s) among all the reported peroxidase mimics to date, including the pristine AgFKZSiW12 (2 min). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00163hDOI Listing

Recent advances in open tubular capillary liquid chromatography.

Analyst 2019 Apr 12. Epub 2019 Apr 12.

ASTech, ARC Training Centre for Portable Analytical Separation Technologies (ASTech), and Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.

This review covers advances and applications of open tubular capillary liquid chromatography (OT-LC) over the period 2007-2018. Under the right conditions OT-LC columns have the potential to offer superior column efficiency, higher overall peak capacity, and higher column permeability compared to packed capillary and monolithic columns. However, such advantages are highly dependent upon column format and dimensions, and to date in liquid chromatography the advantages of open tubular format columns have been most widely discussed and applied in the field of proteomics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00329kDOI Listing
April 2019
2 Reads

Analysis of bodily fluids using vibrational spectroscopy: a direct comparison of Raman scattering and infrared absorption techniques for the case of glucose in blood serum.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

FOCAS Research Institute, Technological University Dublin, Kevin Street, Dublin 8, Ireland.

Analysis of biomarkers present in the blood stream can potentially deliver crucial information on patient health and indicate the presence of numerous pathologies. The potential of vibrational spectroscopic analysis of human serum for diagnostic purposes has been widely investigated and, in recent times, infrared absorption spectroscopy, coupled with ultra-filtration and multivariate analysis techniques, has attracted increasing attention, both clinical and commercial. However, such methods commonly employ a drying step, which may hinder the clinical work flow and thus hamper their clinical deployment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00125eDOI Listing

Lateral flow assay ruler for quantitative and rapid point-of-care testing.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, Pennsylvania 19104, USA.

Lateral flow assay (LFA) is a well-established platform for point-of-care (POC) testing due to its low cost and user friendliness. Conventional LFAs provide qualitative or semi-quantitative results and require dedicated instruments for quantitative detection. Here, we developed an "LFA ruler" for quantitative and rapid readout of LFA results, using a 3D printed strip cassette and a simple, inexpensive microfluidic chip. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00374fDOI Listing

A quadruple-channel fluorescent sensor array based on label-free carbon dots for sensitive detection of tetracyclines.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400700, P. R. China.

A quadruple-channel fluorescent sensor array based on label-free carbon dots (CDs) was fabricated to detect and discriminate a series of tetracyclines (TCs), including chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TC) and doxycycline (DOX). Blue-emitting carbon dots (B-CDs) and green-emitting carbon dots (G-CDs) were prepared to serve as four sensing elements. When the TCs were directly mixed with CDs, the fluorescence quenching phenomenon appeared. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00148dDOI Listing

Effect of energy per atom (E/n) on the Ar gas cluster ion beam (Ar-GCIB) and O cosputter process.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

Department of Materials Science and Engineering, National Taiwan University, Taipei 106, Taiwan. and Research Center for Applied Science, Academia Sinica, Taipei 115, Taiwan.

Gas cluster ion beam (GCIB) is a promising technique for preserving molecular structures during ion sputtering and successfully profiling biological and soft materials. However, although GCIB yields lower damage accumulation compared with C60+ and monoatomic ion beams, the inevitable alteration of the chemical structure can introduce artifacts into the resulting depth profile. To enhance the ionization yield and further mask damage, a low-energy O2+ (200-500 V) cosputter can be applied. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02452aDOI Listing

Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, 911 Oval Dr., Raleigh, NC 27695, USA. and Department of Electrical and Computer Engineering, North Carolina State University and University of North Carolina at Chapel Hill, 911 Oval Dr., Raleigh, NC 27695, USA.

As microfabrication techniques and tissue engineering methods improve, microphysiological systems (MPS) are being engineered that recapitulate complex physiological and pathophysiological states to supplement and challenge traditional animal models. Although MPS provide unique microenvironments that transcend common 2D cell culture, without proper regulation of oxygen content, MPS often fail to provide the biomimetic environment necessary to activate and investigate fundamental pathways of cellular metabolism and sub-cellular level. Oxygen exists in the human body in various concentrations and partial pressures; moreover, it fluctuates dramatically depending on fasting, exercise, and sleep patterns. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02201aDOI Listing
April 2019
1 Read

Outstanding Reviewers for Analyst in 2018.

Authors:

Analyst 2019 Apr 10. Epub 2019 Apr 10.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an90035gDOI Listing

Solid phase microextraction coupled to mass spectrometry via a microfluidic open interface for rapid therapeutic drug monitoring.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

Department of Chemistry, University of Waterloo, 200 University Avenue west, Waterloo, ON, CanadaN2L 3G1.

Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability. High plasma concentrations have been associated with post-operative seizures. Due to the difficulties with maintaining acceptable concentrations of TXA during surgery, implementation of a point-of-care strategy for testing TXA plasma concentration would allow for close monitoring of its concentration during administration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00041kDOI Listing
April 2019
1 Read

Flexible plasmonic graphene oxide/heterostructures for dual-channel detection.

Analyst 2019 Apr 10. Epub 2019 Apr 10.

Tomsk Polytechnic University, 30 Lenin Ave, 634050 Tomsk, Russia.

Graphene oxide (GO) films are deposited on flexible Kapton substrates and selectively modified to conductive reduced graphene oxide (rGO) electrodes using laser patterning. Based on this, we design, fabricate, and test a flexible sensor integrating laser-reduced GO with silver plasmonic nanostructures. The fabricated device results in dual transduction channels: for electrochemical and plasmonic nanostructure-based surface-enhanced Raman spectroscopy (SERS) detection. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C8AN02495B
Publisher Site
http://dx.doi.org/10.1039/c8an02495bDOI Listing
April 2019
5 Reads

In situ construction of metal-organic framework (MOF) UiO-66 film on Parylene-patterned resonant microcantilever for trace organophosphorus molecules detection.

Analyst 2019 Apr 9. Epub 2019 Apr 9.

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

The detection of organophosphorus (OP) compounds, which are extremely toxic, is a requirement in many application fields such as food security. Mass-type chemical sensors based on ultra-sensitive resonant microcantilevers exhibit high comparative advantages in OP compound detection. However, it is still a big challenge to construct a sensing film in situ from corrosive precursors on resonant cantilevers for batch fabrication. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C8AN02508H
Publisher Site
http://dx.doi.org/10.1039/c8an02508hDOI Listing
April 2019
2 Reads
4.107 Impact Factor

Gold nanocluster-based fluorescence sensing probes for detection of dipicolinic acid.

Analyst 2019 Apr 5. Epub 2019 Apr 5.

Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.

Bacillus spp. are spore-forming bacteria, and some of them, including Bacillus cereus and Bacillus anthracis, are pathogens. Dipicolinic acid (DPA) has been recognized as a biomarker for spore-forming bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00240eDOI Listing
April 2019
4.107 Impact Factor

A simple and low-cost screen printed electrode for hepatocellular carcinoma methylation detection.

Analyst 2019 Apr 3. Epub 2019 Apr 3.

College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, PR China.

There is a great demand for robust diagnostic and prognostic approaches for Hepatocellular Carcinoma (HCC). DNA methylation, a common epigenetic modification, has been found in many promoter regions of tumor suppressor genes. Hypermethylation of these gene promoters will repress the gene transcription and lead to the occurrence of cancers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00191cDOI Listing
April 2019
3 Reads

A physiometer for simultaneous measurement of whole blood viscosity and its determinants: hematocrit and red blood cell deformability.

Analyst 2019 Apr 3. Epub 2019 Apr 3.

Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea. and School of Mechanical Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.

In this study, a microfluidic-based physiometer capable of measuring whole blood viscosity, hematocrit, and red blood cell (RBC) deformability on a chip is introduced. The physiometer consists of two major parts: a hydrodynamic component for whole blood viscosity measurement and an electronic component for hematocrit and RBC deformability measurement. In the hydrodynamic component, the whole blood is infused with phosphate buffered saline as a reference fluid for estimation of the whole blood viscosity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02135jDOI Listing
April 2019
2 Reads

Single-cell analysis reveals the effects of glutaraldehyde and formaldehyde on individual Nosema bombycis spores.

Analyst 2019 Apr 3. Epub 2019 Apr 3.

Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.

Nosema bombycis (Nb) is the pathogen that causes pebrine in silkworms. Aldehydes are effective disinfectants commonly used in sericulture. However, the precise mechanism of their action on Nb spores remains unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02425aDOI Listing
April 2019
2 Reads

Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular cartilage.

Analyst 2019 Apr 2. Epub 2019 Apr 2.

Inserm, UA7, Rayonnement Synchrotron pour la Recherche Biomédicale (STROBE), France and Université Grenoble Alpes, Grenoble, France and European Synchrotron Radiation Facility, F38043, Grenoble Cedex 9, France.

The speciation of highly-diluted elements by X-ray absorption spectroscopy in a diverse range of materials is extremely challenging, especially in biological matrices such as articular cartilage. Here we show that using a high energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) technique coupled to an array of crystal analyzers, selenium speciation down to 400 ppb (μg kg-1) within articular cartilage can be demonstrated. This is a major advance in the speciation of highly-diluted elements through X-ray absorption spectroscopy and opens new possibilities to study the metabolic role of selenium and other elements in biological samples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00207cDOI Listing

Label-free density-based detection of adipocytes of bone marrow origin using magnetic levitation.

Analyst 2019 Apr 2. Epub 2019 Apr 2.

Department of Bioengineering, Izmir Institute of Technology, Urla, Izmir, Turkey.

Adipocyte hypertrophy and hyperplasia are important parameters in describing abnormalities in adipogenesis that are concomitant to diseases such as obesity, diabetes, anorexia nervosa and osteoporosis. Therefore, technical developments in the detection of adipocytes become an important driving factor in adipogenesis research. Current techniques such as optical microscopy and flow cytometry are available in detection and examination of adipocytes, driving cell- and molecular-based research of adipogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02503gDOI Listing
April 2019
1 Read

Preparation of salbutamol imprinted magnetic nanoparticles via boronate affinity oriented surface imprinting for the selective analysis of trace salbutamol residues.

Analyst 2019 Apr 1. Epub 2019 Apr 1.

Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu 233000, China. and School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu 233000, China.

Salbutamol (SAL) is one of the most widely abused feed additives in animal husbandry. Selective enrichment of SAL from complex biological samples is a key step for the analysis of SAL. Herein, we present SAL-imprinted magnetic nanoparticles (MNPs) as an ideal sorbent for selective enrichment of SAL. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00198kDOI Listing

Immobilization of ssDNA on a metal-organic framework derived magnetic porous carbon (MPC) composite as a fluorescent sensing platform for the detection of arsenate ions.

Analyst 2019 Mar 29. Epub 2019 Mar 29.

Department of Applied Science and Technology, A.C. Tech, Anna University, Chennai, 600 025, India.

In this work, we fabricated a metal-organic framework derived magnetic porous carbon (MPC) composite using a one-pot solid state template method. The formation of the synthesized composite was confirmed with various spectroscopic techniques, and it was proved that the composite can effectively quench the fluorescence of ssDNA. This property was utilized in the specific and efficient recognition of harmful arsenate ions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00139eDOI Listing

Iron(iii)-selective materials based on a catechol-bearing amide for optical sensing.

Analyst 2019 Mar 29. Epub 2019 Mar 29.

Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdansk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland.

The synthesis and ion-binding properties of a new amide L derived from 3,4-dihydroxybenzoic acid are described. Due to the presence of a catechol unit, the compound interacts selectively with iron(iii) in organic solvent (dimethyl sulfoxide, DMSO) to produce a color change from pale yellow to green. The incorporation of the ligand L into polymeric matrices or its encapsulation into surfactant-based spheres enables analyte detection in aqueous solutions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00188cDOI Listing

Nonlinear microscopy of common histological stains reveals third harmonic generation harmonophores.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

Department of Chemistry, McGill University, 801 Sherbrooke St West, Montreal, QC H3A 0B8, Canada. and Department of Physics, McGill University, 3600 University St, Montreal, QC H3A 2T8, Canada.

Since its invention over a hundred years ago, histological analysis using coloured dye staining remains the gold standard for histopathology. While these stains provide critical information for a variety of diagnostic purposes, they offer limited two-dimensional histological information. Extending classical histological analysis to three dimensions requires novel imaging approaches such as multiphoton microscopy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00267gDOI Listing

Multifunctional titanium phosphate nanoparticles for site-specific drug delivery and real-time therapeutic efficacy evaluation.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. and Hanlin College, Nanjing University of Chinese Medicine, Taizhou 225300, PR China.

Receptor-targeted delivery systems have been proposed as means of concentrating therapeutic agents to improve therapeutic effects on disease sites and reduce side effects on normal issues. Herein, we synthesized biocompatible folic acid (FA)-functionalized DHE-modified TiP (TiP-PAH-DHE-FA) nanoparticles as a drug delivery system that possessed high drug loading capability and enhanced folate-receptor-mediated cellular uptake. Moreover, it also allowed drug effect evaluation based on the real-time monitoring of the fluorescence intensity of HE molecules that are triggered by intercellular ROS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02450bDOI Listing
March 2019
4.107 Impact Factor

Dual-binding pyridine and rhodamine B conjugate derivatives as fluorescent chemosensors for ferric ions in aqueous media and living cells.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

College of Science, Nanjing Forestry University, Nanjing 210037, China.

Two new pyridine-type rhodamine B chemosensors (RBPO and RBPF) used to detect Fe3+ have been designed and synthesized, and the sensing behavior towards various metal ions was evaluated via UV-vis and fluorescence spectroscopic techniques. Both RBPO and RBPF not only have good spectral responses to Fe3+ in an EtOH/H2O solution (3 : 1, v/v, HEPES, 0.5 mM, pH = 7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an01915kDOI Listing

A monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.

Imidacloprid (IMD) is one of the most used pesticides worldwide as a systemic insecticide as well as for pest control and seed treatment. The toxic and potential carcinogenic character of IMD makes its monitoring of great relevance in the field of agriculture and environment, so sensitive methodologies for in field analysis are strongly required. In this context, we have developed a competitive immunoassay for the determination of IMD using specific monoclonal antibodies followed by electrochemical detection on screen-printed carbon electrodes (SPCE). Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00176J
Publisher Site
http://dx.doi.org/10.1039/c9an00176jDOI Listing
March 2019
1 Read

Ultrasensitive SERS detection of specific oligonucleotides based on Au@AgAg bimetallic nanorods.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. and Institute of Engineering Biology and Health, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China and School of Chemistry and Chemical Engineering, Shihezi University, Xinjiang, 832000, China.

We synthesized a novel and sensitive Au/Ag bimetallic SERS-active nanotag, Au-Ag-Ag core-shell-shell nanorod (Au@AgAgNR). The Au@AgAgNR nanotag exhibited a strong SERS signal and was easily assembled from bilayer silver shells on an Au nanorod (AuNR) core with embedded Raman reporter molecules in the core-shell-shell gaps. The SERS activity of the nanotags was investigated with 2-mercaptopyridine (2-Mpy) as a Raman reporter, which could form pyridine/Ag+ coordination complexes to mediate the formation of silver shells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00306aDOI Listing
March 2019
1 Read

Microfluidic analysis of fentanyl-laced heroin samples by surface-enhanced Raman spectroscopy in a hydrophobic medium.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

Department of Mechanical Engineering, University of California Santa Barbara, Santa Barbara, California 93106, USA.

Opioid overdose deaths resulting from heroin contaminated with the potent opioid agonist fentanyl, are currently a serious public health issue. A rapid and reliable method for identifying fentanyl-laced heroin could lead to reduced opioid overdose. Herein, we describe a strategy for detecting fentanyl at low concentrations in the presence of heroin, based on the significant hydrophobicity of fentanyl compared to heroin hydrochloride, by preferentially extracting trace concentrations of fentanyl using ultrasound-assisted emulsification microextraction using octanol as the extracting phase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00168aDOI Listing

An ultrasensitive and simple fluorescence biosensor for detection of the Kras wild type by using the three-way DNA junction-driven catalyzed hairpin assembly strategy.

Analyst 2019 Mar 28. Epub 2019 Mar 28.

Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-Environmental and Science &Technology, Guangzhou 510650, China.

In this work, a label-free fluorescence biosensor was proposed for simple detection of the Kras wild type by using the three way DNA junction-driven catalyzed hairpin assembly strategy. In this system, a three-way DNA junction probe (JP) and two hairpin probes (H1 and H2) were designed. In the presence of the Kras wild type, an autocatalytic DNA machine can be activated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00195fDOI Listing

Nanogold hybrid silica gel and its 1-octadecanethiol self-assembled modified composite as a stationary phase for liquid chromatography.

Analyst 2019 Mar 27. Epub 2019 Mar 27.

Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

Au nanoparticle-hybridized silica (Au@sil) spheres were synthesized in one step as a liquid chromatographic stationary phase for the first time. The hybridized stationary phase showed good separation performances in reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography even without bonding with any organic groups. Compared with the bare silica stationary phase, the Au@sil stationary phase showed better separation performance under the same conditions in RPLC and HILIC modes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00270gDOI Listing

Base excision repair mediated cascading triple-signal amplification for the sensitive detection of human alkyladenine DNA glycosylase.

Analyst 2019 Mar 27. Epub 2019 Mar 27.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

DNA glycosylase (DG) plays a significant role in repairing DNA lesions, and the dysregulation of DG activity is associated with a variety of human pathologies. Thus, the detection of DG activity is essential for biomedical research and clinical diagnosis. Herein, we develop a facile fluorometric method based on the base excision repair (BER) mediated cascading triple-signal amplification for the sensitive detection of DG. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00200F
Publisher Site
http://dx.doi.org/10.1039/c9an00200fDOI Listing
March 2019
6 Reads

Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays.

Analyst 2019 Mar 27. Epub 2019 Mar 27.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

MicroRNA (MiRNA) plays a crucial role in biological cells to enable assessment of a cancer's development stage. Increasing evidence has shown that the accurate and sensitive detection of miRNA holds the key toward correct disease diagnosis. However, some characteristics of miRNAs, such as their short chains, low concentration, and similar sequences, make it difficult to detect miRNA in biological samples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00081jDOI Listing

Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model.

Analyst 2019 Mar 27. Epub 2019 Mar 27.

TU Wien, Institute of Materials Science and Technology, Getreidemarkt 9, 1060 Vienna, Austria. and Austrian Cluster for Tissue Regeneration, Austria.

Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C9AN00068B
Publisher Site
http://dx.doi.org/10.1039/c9an00068bDOI Listing
March 2019
3 Reads

Determination of nitrated polycyclic aromatic hydrocarbons in particulate matter 2.5 by laser ionization mass spectrometry using an on-line chemical-reduction system.

Analyst 2019 Mar 26. Epub 2019 Mar 26.

Division of International Strategy, Center of Future Chemistry, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Nitrated polycyclic aromatic hydrocarbons (NPAHs) were separated by gas chromatography (GC) and were introduced into a reactor consisting of an inner-seal Y-connector for the on-line chemical reduction of NPAH using hydrazine monohydrate as a chemical reducing agent. The reduced forms of amino polycyclic aromatic hydrocarbons (APAHs) were then determined by multiphoton ionization time-of-flight mass spectrometry (MPI/TOF-MS) using a femtosecond laser as the ionization source. Three standard samples of NPAHs, i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00308hDOI Listing

Frequency division multiplex HPLC-MS for simultaneous analyses.

Analyst 2019 Mar 26. Epub 2019 Mar 26.

Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.

Multiplex high-performance liquid chromatograph-mass spectrometry (HPLC-MS), in which multiple HPLCs and one MS are hyphenated, is an approach for high throughput analysis in HPLC-MS. A general multiplex HPLC-MS method employs a column-switching technology, and only one HPLC is connected to one MS at a time. In the present study, we propose a novel multiplex HPLC-MS system for simultaneous HPLC-MS analyses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02352bDOI Listing

Nanoskiving fabrication of size-controlled Au nanowire electrodes for electroanalysis.

Analyst 2019 Mar 26. Epub 2019 Mar 26.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190, China.

Nanoskiving, benefiting from its simple operation and high reproducibility, is a promising method to fabricate nanometer-size electrodes. In this work, we report the fabrication of Au nanowire electrodes with different shapes and well-controlled sizes through nanoskiving. Au nanowire block electrodes, membrane electrodes and tip electrodes are prepared with good reproducibility. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00122kDOI Listing

An improved scoring method for the identification of endogenous peptides based on the Mascot MS/MS ion search.

Analyst 2019 Mar 26. Epub 2019 Mar 26.

Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan 11529.

To identify endogenous peptides using MS/MS analysis and searching against a polypeptide sequence database, a non-enzyme specific (NES) search considering all of the possible proteolytic cleavages is required. However, the use of a NES search generates more false positive hits than an enzyme specific search, and therefore shows lower identification performance. In this study, the use of the sub-ranked matches for improving the identification performance of the Mascot NES search was investigated and a new scoring method was developed that considered the contribution of all sub-ranked random match probabilities, named the contribution score (CS). Read More

View Article

Download full-text PDF

Source
http://xlink.rsc.org/?DOI=C8AN02141D
Publisher Site
http://dx.doi.org/10.1039/c8an02141dDOI Listing
March 2019
3 Reads

Capillary photoionization: interface for low flow rate liquid chromatography-mass spectrometry.

Analyst 2019 Mar 26. Epub 2019 Mar 26.

Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, 00014 Finland.

This is the first report on capillary photoionization (CPI) interfacing a liquid chromatograph (LC) and mass spectrometer (MS). A new heated CPI ion source was developed, including a heated transfer capillary, a wide oval-shaped and low-depth ionization chamber with a vacuum ultraviolet (VUV) transparent magnesium fluoride (MgF2) window to increase the photoionization efficiency and thus the sensitivity. As both analytes and eluent are first vaporized and then photoionized inside the CPI ion source between the atmosphere and the vacuum of MS, the ion transfer efficiency into the MS and thus the sensitivity is improved. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9an00258hDOI Listing

Direct glucose detection in whole blood by colorimetric assay based on glucose oxidase-conjugated graphene oxide/MnO nanozymes.

Analyst 2019 Mar 25. Epub 2019 Mar 25.

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.

We herein report a facile approach for the preparation of horseradish peroxidase (HRP)-mimic glucose oxidase-conjugated graphene oxide/MnO2 (GOD-GO/MnO2) as new nanozyme to detect the glucose concentration in whole blood. The nano-sized of MnO2 nanoparticles embedded in bovine serum albumin (BSA)-coated GO by in situ growth were evaluated focusing on the principle of HRP-mimic activity catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. Furthermore, we constructed dual sensing platforms based on the combination of a plasma separation pad and GOD-GO/MnO2 for direct detection of glucose concentration in whole blood by colorimetric assay without blood sample pretreatment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an02440eDOI Listing
March 2019
1 Read
4.107 Impact Factor