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Anaerobe 2019 Apr 16. Epub 2019 Apr 16.

Department of Microbiology and Instituto Biosanitario, University Hospital Virgen de las Nieves., Granada, Spain. Electronic address:

Eggerthia catenaformis is a Gram-positive anaerobic rod, which has been rarely reported in human diseases. We report the second case of bacteremia due to this microorganism in an elderly patient. A 73-year-old man, without underlying diseases presented with fever, odynophagia and swelling of the cervical lymph node for several days. Read More

Anaerobe 2019 Apr 14. Epub 2019 Apr 14.

Franklin-Wilkins Building, King's College London, London, SE1 9NH, UK. Electronic address:

Anaerobe 2019 Apr 10. Epub 2019 Apr 10.

EA 4065, Faculty of Pharmacy, Paris Descartes University, Paris, France. Electronic address:

Necrotizing enterocolitis (NEC) is the most severe life threatening gastrointestinal disease among preterm neonates. NEC continues to account for substantial morbidity and mortality in neonatal intensive care units worldwide. Although its pathogenesis remains incompletely elucidated, NEC is recognized as a multifactorial disease involving intestinal unbalanced inflammatory response, feeding strategies, and bacterial colonization. Read More

Anaerobe 2019 Apr 6;57:107-114. Epub 2019 Apr 6.

Southern Right Whale Health Monitoring Program, Chubut, 9120, Argentina; School of Veterinary Medicine, University of California Davis, California, 95616, United States.

Between 2003 and 2017, at least 706 southern right whale (Eubalaena australis) calves died at the Península Valdés calving ground in Argentina. Pathogenic microbes are often suggested to be the cause of stranding events in cetaceans; however, to date there is no evidence supporting bacterial infections as a leading cause of right whale calf deaths in Argentina. We used high-throughput sequencing and culture methods to characterize the bacterial communities and to detect potential pathogens from the intestine of stranded calves. Read More

Anaerobe 2019 Apr 5;57:93-98. Epub 2019 Apr 5.

Servicio de Enfermedades Infecciosas, Hospital Universitario Ramón y Cajal, IRYCIS, Spain. Electronic address:

Clostridium difficile infection (CDI) is characterized by a high delayed and unrelated mortality. Predicting delayed mortality in CDI patients could allow the implementation of interventions that could reduce these events. A prospective multicentric study was carried out to investigate prognostic factors associated with mortality. Read More

Anaerobe 2019 Apr 4;57:90-92. Epub 2019 Apr 4.

Department of Laboratory Medicine, Saitama Medical University International Medical Center, Saitama, Japan. Electronic address:

We report the case of a patient with advanced gastric cancer and multiple liver metastases, who presented with bacteremia and hepatic gas gangrene caused by Clostridium novyi (C. novyi). The gas gangrene caused abscesses to form within metastatic lesions. Read More

Anaerobe 2019 Apr 4;57:99-106. Epub 2019 Apr 4.

Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan, 31538, Republic of Korea; Department of Medical Sciences, College of Medical Sciences, Soonchunhyang University, Asan, 31538, Republic of Korea. Electronic address:

Enterotoxigenic Bacteroides fragilis (ETBF) can form biofilms in the colon mucosal membrane and may promote chronic infection and tumor formation. The objective of this study was to determine the effect of zerumbone extracted from Zingiber zerumbet (L.) Smith, on B. Read More

Anaerobe 2019 Apr 2;57:86-89. Epub 2019 Apr 2.

Department of Infection Control and Prevention, Aichi Medical University Hospital, Aichi, Japan; Department of Clinical Infectious Diseases, Aichi Medical University Graduate School of Medicine, Aichi, Japan. Electronic address:

Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can cause aspiration pneumonia, Fusobacterium spp. Read More

Anaerobe 2019 Apr 2;57:82-85. Epub 2019 Apr 2.

Department of Surgery, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India.

Fusobacterium is a gram negative obligate anaerobic bacilli, a normal inhabitant of gastrointestinal tract, oropharynx and female genital tract. Here we report a case of Fourniers gangrene from which Fusobacterium varium has been isolated along with certain other pathogens. There are only a few reported cases of Fusobacterium varium in literature and it has never been reported from Fournier's gangrene. Read More

Anaerobe 2019 Apr 1. Epub 2019 Apr 1.

Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, 86011, USA; Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, 86011, USA. Electronic address:

Clostridioides difficile infection (CDI) is an emerging public health threat and C. difficile is the most common cause of antimicrobial-associated diarrhea worldwide and the leading cause of hospital-associated infections in the US, yet the burden of community-acquired infections (CAI) is poorly understood. Characterizing C. Read More

Anaerobe 2019 Mar 29;57:75-81. Epub 2019 Mar 29.

Bacteriology Department, CHU Nantes, CRCINA, University Nantes, Nantes, France. Electronic address:

Seven protocols were tested to prepare cell wall extracts from live Cutibacterium acnes. Different parameters were modified: thawing/freezing and sonication/freezing cycles, to impact on mechanical degradation of the bacteria. Finally, the immunogenic potential of the extracts generated was evaluated by measuring IL-8 releases using an in vitro skin explants system. Read More

Anaerobe 2019 Mar 26. Epub 2019 Mar 26.

Department of Biology, Texas A&M University, College Station, TX, 77843, USA. Electronic address:

The germination of Clostridium difficile spores is an important stage of the C. difficile life cycle. In other endospore-forming bacteria, the composition of the medium in which the spores are generated influences the abundance of germination-specific proteins, thereby influencing the sensitivity of the spores towards germinants. Read More

Anaerobe 2019 Mar 26;57:59-62. Epub 2019 Mar 26.

Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Huddinge, Sweden. Electronic address:

Identification of anaerobic bacteria causing blood stream infections (BSI) is challenging. This study describes the epidemiology of anaerobic BSI at a tertiary care hospital and the performance of a rapid method for identification of anaerobic bacteria from blood cultures over three years, between June 2015 and June 2018. Short-term culturing is a low-cost user-friendly method and may be used for rapid identification of bacteria from positive blood cultures by matrix-associated laser desorption ionization time of flight (MALDI-TOF). Read More

Anaerobe 2019 Mar 26;57:63-74. Epub 2019 Mar 26.

Microbiology Division, Defence Food Research Laboratory, Mysore, 570011, India. Electronic address:

The prevalence and lethality associated with C. perfringens alpha (CPA) and enterotoxin (CPE) toxaemia necessitate the need for rapid and definitive detection systems to initiate management measures. In the present study, a sandwich duplex immuno-capture PCR (SD-IPCR) was developed by employing IgY antibodies against a bivalent protein r-Cpae derived from CPA and CPE for antigen capture and reporter antibodies against truncated CPA or CPE conjugated to oligomers of distinguishable size for antigen revealing and signal amplification. Read More

Anaerobe 2019 Mar 18;57:55-58. Epub 2019 Mar 18.

Department of Animal Health, Faculty of Veterinary Science, Universidad Complutense de Madrid, Madrid, Spain; Hospital Clinico Veterinario Complutense, Universidad Complutense, Madrid, Spain.

The present study focused on detecting the presence of Clostridium difficile on veterinary hospital surfaces of large and small animal areas at the Universidad Complutense of Madrid. Isolated C. difficile strains were further characterized and investigated for antimicrobial susceptibility testing. Read More

Anaerobe 2019 Mar 14;57:35-38. Epub 2019 Mar 14.

Queen's University, Kingston, ON, Canada.

Clostridium (Clostridioides) difficile has been identified in humans and a wide range of animal species, but there has been little study of remote animal populations with limited human contact. The objective of this study was to determine the prevalence and types of C. difficile in wild and captive polar bears (Ursus maritimus). Read More

Anaerobe 2019 Mar 15;57:45-54. Epub 2019 Mar 15.

Department of Molecular Microbiology, Tokyo University of Agriculture, Tokyo, Japan; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Bifidobacterium is beneficial for host health and exhibits different O sensitivity levels among species or strains via unknown mechanisms. Bifidobacterium bifidum JCM1255, a type species of Bifidobacterium, is an O-sensitive bacterium that can grow under low-O (5%) conditions, and the growth of this species is inhibited under high-O conditions (10% ∼) with accumulation of HO. We previously reported that NADH or NAD(P)H oxidase-active fractions were detected during purification using microaerobically grown B. Read More

Anaerobe 2019 Mar 13;57:39-44. Epub 2019 Mar 13.

Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine-University, Düsseldorf, Germany.

Objectives: Increasing incidence and severity of Clostridium difficile infection (CDI) in the last decades has been attributed to the emergence of hypervirulent C. difficile strain PCR-ribotype 027 (RT027). Commercial multiplex real-time PCR tests allow the presumptive identification of RT027 by detecting a single-base deletion at nt117 in the tcdC gene (tcdCΔ117). Read More

Anaerobe 2019 Mar 12;57:28-31. Epub 2019 Mar 12.

Faculty of Natural Resource Science, University of Akureyri, Borgir, Nordurslod 2, 600, Akureyri, Iceland. Electronic address:

Higher order alcohols, such as 1-butanol and 1-hexanol, have a large number of applications but are currently prepared from non-renewable feedstocks. Here, the ability of Thermoanaerobacter pseudoethanolicus to reduce short-chain fatty acids to their corresponding alcohols using reducing potential generated by glucose catabolism with yields between 21.0 and 61. Read More

Anaerobe 2019 Mar 12;57:11-18. Epub 2019 Mar 12.

Biotechnology Division, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bang Khun Thian, Bangkok, 10150, Thailand. Electronic address:

In anaerobic wastewater treatment, microbial biofilm is beneficial for efficient substrate utilization and for preventing the wash-out of key microorganisms. By providing solid supports, biofilm formation can be accelerated due to the early initial adhesion of residing microbes. Alteration in surface properties is therefore one such approach that helps us understand microbial interfacial interaction. Read More

Anaerobe 2019 Mar 12. Epub 2019 Mar 12.

Section of Transplant Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA.

Clostridioides (Clostridium) difficile is the leading cause of healthcare-associated infectious diarrhea in the developed world. Retrospective studies have shown a lower incidence of C. difficile infection (CDI) in Japan than in Europe or North America. Read More

Anaerobe 2019 Mar 11;57:32-34. Epub 2019 Mar 11.

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Septic arthritis is one of the most serious conditions leading to grave consequences in all age groups, with a number of cases seen in infancy and childhood. Although septic arthritis in neonates is a known clinical entity, it is a diagnostic dilemma for the paediatricians. Of the reported causes of bacterial arthritis, anaerobic bacteria account for only 1%. Read More

Anaerobe 2019 Mar 9;57:4-10. Epub 2019 Mar 9.

School of Biomedical Sciences, The University of Western Australia, Western Australia, 6009, Australia; School of Veterinary and Life Sciences, Murdoch University, Western Australia, 6150, Australia; School of Medical and Health Sciences, Edith Cowan University, Western Australia, 6027, Australia; Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Western Australia, 6009, Australia. Electronic address:

Studies on the prevalence and diversity of Clostridium difficile in Thailand have been limited to those derived from a few tertiary hospitals in Central Thailand. In this study, 145 C. difficile isolates collected in 13 provinces in Thailand during 2006-2018 were characterized by ribotyping and detection of toxin genes. Read More

Anaerobe 2019 Apr 6;56:102-105. Epub 2019 Mar 6.

Kelsey Research Foundation, USA; University of Texas School of Public Health, USA; University of Texas McGovern Medical School, USA; Baylor College of Medicine, USA.

Fecal calprotectin and indole were studied in 134 subjects with recurrent CDI before and after FMT. Reduced fecal calprotectin (p = 0.0353, 95% CI 0. Read More

Anaerobe 2019 Mar 7;57:19-27. Epub 2019 Mar 7.

Institute of Animal Nutrition and Functional Plant Compounds, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210, Vienna, Austria; Research Cluster "Animal Gut Health", Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.

This study aimed at testing the effects of three different formulations of feed supplements based on three different combinations of plant derived alkaloids, prebiotics, tannins, vitamins and minerals on rumen fermentation and the microbiome in vitro. A Rusitec experiment was conducted in 2 identical runs using a complete randomized design with 3 replicates per treatment resulting in total of 6 treatment combinations (n = 6). Each run lasted 12 d with sampling occurring in the last 5 d. Read More

Anaerobe 2019 Mar 6. Epub 2019 Mar 6.

Universidade Federal do Rio de Janeiro, IMPG, Depto. de Microbiologia Médica, Rio de Janeiro, Brazil. Electronic address:

Clostridioides difficile is considered one of the main etiological agents of bacterial diarrhea associated with the use of antibiotics. It is an important nosocomial pathogen and the main cause of morbidity and mortality. In recent years, infections associated with C. Read More

Anaerobe 2019 Apr 5;56:116-123. Epub 2019 Mar 5.

Hebei Provincial Center for Clinical Laboratories, Second Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, China; Department of Clinical Laboratory, Second Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, China. Electronic address:

Clostridioides difficile is a colonizer of the human gut; asymptomatic colonization has been reported to be more common in infants and is highly variable across regions even with no symptoms of diarrhea or death. Antibiotic treatment strategies might increase the antibiotic resistance of C. difficile. Read More

Anaerobe 2019 Apr 5;56:109-115. Epub 2019 Mar 5.

Chair and Department of Medical Microbiology, Medical University of Warsaw, Chalubinskiego 5 Str., 02-004, Warsaw, Poland; Department of Medical Microbiology, The Infant Jesus Teaching Hospital, Lindleya 4 Str., 02-004, Warsaw, Poland.

The purpose of this study was to assess drug susceptibility of clinical B. fragilis strains and to determine any correlation between drug resistance and the presence of specific genes. Antimicrobial susceptibility was assessed using E-tests. Read More

Anaerobe 2019 Feb 28;57:1-3. Epub 2019 Feb 28.

Department of Microbiology, University of Washington, Seattle, WA, 98195, USA; Department of Laboratory Medicine, University of Washington, Seattle, WA, 98195, USA; Department of Medicine, University of Washington, Seattle, WA, 98195, USA; Department of Immunology, University of Washington, Seattle, WA, 98195, USA; Department of Genome Sciences, University of Washington, Seattle, WA, 98195, USA.

Clostridium difficile (C. difficile) is an important nosocomial pathogen in adults and children. Roughly 4-5% of non hospitalized healthy adults carry the organism in their intestinal flora while adults in long term care facilities have asymptomatic carriage rates estimated at 20-50%. Read More

Anaerobe 2019 Apr 26;56:106-108. Epub 2019 Feb 26.

Instituto Dante Pazzanese de Cardiologia, Division of Infectious Diseases, São Paulo, SP, Brazil.

Late complications in ascending aortic surgeries are uncommon and may occur by infectious processes, usually caused by gram positive bacteria. We report a case of aortic prosthesis infection by Porphyromonas pogonae, an anaerobic gram-negative coccobacillus that can grow under microaerobic conditions, three years after ascending aortic reconstruction surgery. Read More

Anaerobe 2019 Apr 23;56:130-132. Epub 2019 Feb 23.

LVR-Klinik for Orthopaedic Surgery, Viersen, Germany.

We report on the first case of a periprosthetic joint infection with the anaerobic Gram-positive rod Slackia exigua as the causative agent. The bacterium is part of human oral microbiota and has so far mainly been associated with periodontal diseases. Read More

Anaerobe 2019 Apr 22;56:124-129. Epub 2019 Feb 22.

Division of Microbiology, National Center for Toxicological Research, FDA, Jefferson, AR, 72079, USA. Electronic address:

Clostridium perfringens, an anaerobic, spore-forming bacterium, causes infections in humans and animals by producing several toxins encoded by genes found either on the chromosomes or on diverse plasmids. The plasmids may code for more than one toxin gene or antimicrobial-resistance gene. In this study, the prevalence of the β2, cpe and tpeL toxin genes and the tetA(P), tetB(P) and tetM tetracycline-resistance genes, in 56 strains of C. Read More

Anaerobe 2019 Apr 19;56:88-90. Epub 2019 Feb 19.

University of Houston College of Pharmacy, Houston, TX, USA.

Clostridioides difficile infection (CDI) is an emerging but often understudied infectious disease in developing countries. This study was aimed to isolate and characterize C. difficile from shoe sole swabs and diarrheal patient's stool samples in Bangladesh. Read More

Anaerobe 2019 Apr 20;56:98-101. Epub 2019 Feb 20.

Microhealth Group, Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias - Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Asturias, Spain. Electronic address:

The toll-like receptors involved in recognition of the exopolysaccharide produced by two isogenic, ropy and non-ropy, Bifidobacterium animalis subsp. lactis strains were investigated. Both strains interact with human embryonic kidney (HEK)-293 cells via TLR2, whereas purified EPSs specifically stimulate TLR4 regardless their molar mass. Read More

Anaerobe 2019 Apr 19;56:91-94. Epub 2019 Feb 19.

Section of Microbiology, University Hospital of Guadalajara, Guadalajara, Spain.

We describe the characteristics of 11 cases of skin and soft tissue infections due to Cutibacterium (formerly Propionibacterium) avidum. This microorganism should be considered a potential skin pathogen especially in patients with breast infections. The clinical outcome is usually good but serious infections such as necrotizing infections may occur. Read More

Anaerobe 2019 Apr 16;56:66-77. Epub 2019 Feb 16.

Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia. Electronic address:

Recent reports on antimicrobial effects of metallic Cu prompted this study of anaerobic microbial communities on copper surfaces. Widely circulating copper-containing coinage was used as a potential source for microorganisms that had had human contact and were tolerant to copper. This study reports on the isolation, characterization, and genome of an anaerobic sulfidogenic Tissierella sp. Read More

Anaerobe 2019 Apr 14;56:95-97. Epub 2019 Feb 14.

Marmara University, School of Medicine, Department of Microbiology, Istanbul, Turkey.

We have reported a case of mastoiditis which progressed to meningitis in a 16-year old male patient. CSF (cerebrospinal fluid) anaerobic culture revealed four species of isolated anaerobic bacteria. This is the first case in the literature in which a patient survived childhood polymicrobial anaerobic meningitis diagnosed by MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry). Read More

Anaerobe 2019 Apr 14;56:78-87. Epub 2019 Feb 14.

Vetsuisse Faculty, University of Bern, Bern, Switzerland. Electronic address:

Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the only efficient strategy in order to control the disease. The β-barrel pore forming leucocidin Clostridium chauvoei toxin A (CctA) is one of the best characterised toxins of C. Read More

Anaerobe 2019 Apr 13;56:51-56. Epub 2019 Feb 13.

Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. Electronic address:

Fusobacterium necrophorum is a Gram negative, rod-shaped and aero tolerant anaerobe. In animals, it is an opportunistic pathogen frequently associated with necrotic infections, generally called necrobacillosis, such as calf diphtheria, foot rot and liver abscesses in cattle. Two subspecies exist: subsp. Read More

Anaerobe 2019 Feb 12. Epub 2019 Feb 12.

Division of Oral Microbiology and Immunology, Department for Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University Hospital, Aachen, Germany.

Next-generation sequencing allows for investigating the composition of microbiomes that are associated with infection (clinical microbiology) or dysbiosis (microbial ecology). The most commonly applied short-read sequencing technologies are Illumina MiSeq/HiSeq and Ion Torrent PGM, however, other platforms that generate long-reads are under way and optimized. A pre-condition for representative results is an appropriate method for contamination-free collection, homogenization, storage of specimens and a subsequent efficient DNA extraction protocol. Read More

Anaerobe 2019 Apr 12;56:49-50. Epub 2019 Feb 12.

National Reference Center for Anaerobic Bacteria and Botulism, Pasteur Institute, 75015, Paris, France. Electronic address:

Botulism type C was suspected in a 46-year old man after consumption of sick poultry from a flock where botulism type C was confirmed. The patient developed characteristic signs of botulism, but investigation of biological samples did not confirm the presence of Clostridium botulinum or botulinum toxin. Despite having classical botulism symptoms, the man recovered very quickly. Read More

Anaerobe 2019 Apr 10;56:46-48. Epub 2019 Feb 10.

Laboratory of Microbiology, Department of Infectious Agents, Saint Louis - Lariboisiere - Fernand Widal University Hospital, APHP, Paris, France; IAME, UMR 1137, INSERM, Paris Diderot University, Sorbonne Paris Cité, Paris, France.

Clostridium is a diverse genus including more than 200 species involved in varied clinical presentations in infectious diseases. Septic arthritis caused by Clostridium sp. are however uncommon. Read More

Anaerobe 2019 Apr 10;56:61-65. Epub 2019 Feb 10.

Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA; University of Pittsburgh, Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Hospital, 300 Halket Street, Pittsburgh, PA, 15213, USA. Electronic address:

The CDC recommended outpatient treatment of pelvic inflammatory disease (PID) is an intramuscular dose of ceftriaxone plus 14 days of doxycycline, with or without metronidazole. European guidelines (2017) include moxifloxacin plus ceftriaxone as a first line regimen, particularly for women with Mycoplasma genitalium-associated PID. However, the susceptibility of bacteria recovered from the endometrium of women with PID to moxifloxacin is unknown. Read More

Anaerobe 2019 Apr 7;56:57-60. Epub 2019 Feb 7.

Pediatric Infectious Diseases Unit, Department of Pediatric, King Saud University Medical City, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Spinal intramedullary abscesses are rare and potentially devastating lesions. Infection is mostly caused by staphylococci. Anaerobes are rare and often polymicrobial. Read More

Anaerobe 2019 Apr 6;56:40-45. Epub 2019 Feb 6.

Animal Nutrition Division, Indian Council of Agricultural Research - National Dairy Research Institute, Karnal, 132001, Haryana, India. Electronic address:

In the present study, we investigated the potential of Bifidobacterium spp., isolated from ruminal fluid samples from buffaloes (Bubalus bubalis) for conjugated linoleic acid (CLA) production. A total of 294 isolates were obtained from 86 ruminal fluid samples using Bifidus Selective Medium (BSM) medium, and based on phospoketolase assay, 24 isolates were presumptively confirmed to be Bifidobacterium species. Read More

Anaerobe 2018 Oct;53

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, 02111, USA.

Anaerobe 2019 Feb 1. Epub 2019 Feb 1.

Department of Infectious Diseases, University Medical Centre Ljubljana, Ljubljana, Slovenia.

Bacteroides fragilis can be classified into division I (cfiA negative) and division II (cfiA positive) isolates. Division II isolates have a silent chromosomal carbapenemase gene (cfiA) that can become overexpressed by an insertion of a mobile genetic element and thus develop a phenotypic resistance to carbapenems. Aims of our study were (i) to determine the prevalence of B. Read More

Anaerobe 2019 Apr 29;56:34-39. Epub 2019 Jan 29.

Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil. Electronic address:

The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012-2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (ST), six (30%) of which were novel, revealing a high diversity in a single hospital. Classic hypervirulent strains ST1/RT027 and ST11/RT078 were not identified, while ST42 (almost all RT106) was the most common type, being detected in 11 (28. Read More

Anaerobe 2019 Apr 22;56:133-134. Epub 2019 Jan 22.

Geriatric Research Education and Clinical Center, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, USA; Case Western Reserve University School of Medicine, Cleveland, OH, USA. Electronic address:

Anaerobe 2019 Apr 8;56:22-26. Epub 2019 Jan 8.

Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany; Freie Universität Berlin, Department of Veterinary Medicine, Institute for Virology, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany. Electronic address:

We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed. Read More