4,067 results match your criteria Amino acids[Journal]


Critical functions of the polyamine putrescine for proliferation and viability of Leishmania donovani parasites.

Amino Acids 2019 Apr 16. Epub 2019 Apr 16.

Pacific University School of Pharmacy, Hillsboro, OR, 97123, USA.

Polyamines are metabolites that play important roles in rapidly proliferating cells, and recent studies have highlighted their critical nature in Leishmania parasites. However, little is known about the function of polyamines in parasites. To address this question, we assessed the effect of polyamine depletion in Leishmania donovani mutants lacking ornithine decarboxylase (Δodc) or spermidine synthase (Δspdsyn). Read More

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http://dx.doi.org/10.1007/s00726-019-02736-zDOI Listing

Knocking down raptor in human keratinocytes affects ornithine decarboxylase in a post-transcriptional Manner following ultraviolet B exposure.

Amino Acids 2019 Apr 10. Epub 2019 Apr 10.

Division of Science, The Pennsylvania State University Berks Campus, Reading, PA, 19610, USA.

Non-melanoma skin cancer (NMSC) is the most common form of cancer. Ultraviolet-B (UVB) radiation has been shown to be a complete carcinogen in the development of NMSC. The mammalian target of rapamycin complex 1 (mTORC1) is upregulated by UVB. Read More

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http://link.springer.com/10.1007/s00726-019-02732-3
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http://dx.doi.org/10.1007/s00726-019-02732-3DOI Listing
April 2019
3 Reads

Diversity of advanced glycation end products in the bovine milk proteome.

Amino Acids 2019 Feb 13. Epub 2019 Feb 13.

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Leipzig, Germany.

Milk processing relies on thermal treatments warranting microbiologically safe products with extended shelf life. However, elevated temperatures favor also Maillard reactions yielding the structurally diverse advanced glycation end products (AGEs). AGEs may alter protein functions and immunogenicity and also decrease the nutritional value of milk products. Read More

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http://dx.doi.org/10.1007/s00726-019-02707-4DOI Listing
February 2019
3 Reads

Biotechnological applications of nanostructured hybrids of polyamine carbon quantum dots and iron oxide nanoparticles.

Amino Acids 2019 Apr 2. Epub 2019 Apr 2.

Department of Comparative Biomedicine and Food Science, Agripolis Campus, University of Padua, viale dell'Università 16, 35020, Legnaro, PD, Italy.

The combination of different nanomaterials has been investigated during the past few decades and represents an exciting challenge for the unexpected emerging properties of the resulting nano-hybrids. Spermidine (Spd), a biogenic polyamine, has emerged as a useful functional monomer for the development of carbon quantum dots (CQDs). Herein, an electrostatically stabilized ternary hybrid, constituted of iron oxide-DNA (the core) and spermidine carbon quantum dots (CQD, the shell), was self-assembled and fully characterized. Read More

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http://dx.doi.org/10.1007/s00726-019-02721-6DOI Listing
April 2019
1 Read

Glycine protects partial liver grafts from Kupffer cell-dependent ischemia-reperfusion injury without negative effect on regeneration.

Amino Acids 2019 Apr 2. Epub 2019 Apr 2.

Department of General, Visceral, and Transplant Surgery, University Hospital Graz, Medical University of Graz, Auenbruggerplatz 29, 8036, Graz, Austria.

Donor preconditioning with glycine prevents Kupffer cell-dependent reperfusion injury to liver grafts. Partial liver grafts need to regenerate and grow in size after transplantation; however, glycine inactivates Kupffer cells, which are important for hepatic regeneration. Thus, this study was designed to evaluate the impact of donor preconditioning with glycine after partial liver transplantation (pLTx). Read More

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http://dx.doi.org/10.1007/s00726-019-02722-5DOI Listing
April 2019
3 Reads

A comprehensive toxicity evaluation of novel amino acid-modified magnetic ferrofluids for magnetic resonance imaging.

Amino Acids 2019 Mar 26. Epub 2019 Mar 26.

Department of Chemistry, Sunandan Divatia School of Science, NMIMS University, Vile Parle (West), Mumbai, 400056, India.

Stem cells have been widely exploited as remedial agents in regenerative medicine due to its tremendous potential in treatment of various debilitating diseases. In spite of this fact, there is need of a reliable, clinically applicable cell tracker for deciphering the homing and distribution of stem cells post-transplantation. Researchers have proposed the use of superparamagnetic magnetite (FeO) nanoparticles for in vivo and in vitro tracking and imaging of stem cells. Read More

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http://dx.doi.org/10.1007/s00726-019-02726-1DOI Listing
March 2019
2 Reads

Plasma ADMA, urinary ADMA excretion, and late mortality in renal transplant recipients.

Amino Acids 2019 Mar 26. Epub 2019 Mar 26.

Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, Sector A, University of Groningen, PO Box 30.001, Hanzeplein 1, 9700 RB, Groningen, The Netherlands.

Cardiovascular disease (CVD) is the leading cause of death in renal transplant recipients (RTR). Elevated plasma asymmetric dimethylarginine (pADMA), an endogenous nitric oxide synthase inhibitor produced from the turnover of methylated arginine moieties in proteins, is a risk factor for CVD and mortality. It is unknown how urinary ADMA excretion (uADMA), one of the main ADMA elimination routes, is associated with long-term survival. Read More

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http://dx.doi.org/10.1007/s00726-019-02725-2DOI Listing

The therapeutic and nutraceutical potential of agmatine, and its enhanced production using Aspergillus oryzae.

Amino Acids 2019 Mar 26. Epub 2019 Mar 26.

Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Japan.

Agmatine, a natural polyamine produced from arginine by arginine decarboxylase, was first discovered in 1910, but its physiological significance was disregarded for a century. The recent rediscovery of agmatine as an endogenous ligand for α-adrenergic and imidazoline receptors in the mammalian brain suggests that this amine may be a promising therapeutic agent for treating a broad spectrum of central nervous system-associated diseases. In the past two decades, numerous preclinical and several clinical studies have demonstrated its pleiotropic modulatory functions on various molecular targets related to neurotransmission, nitric oxide synthesis, glucose metabolism, polyamine metabolism, and carnitine biosynthesis, indicating potential for therapeutic applications and use as a nutraceutical to improve quality of life. Read More

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http://dx.doi.org/10.1007/s00726-019-02720-7DOI Listing
March 2019
3.293 Impact Factor

The self-disproportionation of enantiomers (SDE) of amino acids and their derivatives.

Amino Acids 2019 Mar 22. Epub 2019 Mar 22.

Molecular Structure Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.

This review covers the phenomenon of the self-disproportionation of enantiomers (SDE) of amino acids and their derivatives in all its guises from phase transformations (recrystallization, sublimation, and distillation), to the application of force fields, through to chromatography including HPLC, MPLC, gravity-driven column chromatography, and SEC. The relevance of the SDE phenomenon to amino acid research and to marketed pharmaceuticals is clear given the potential for alteration of the enantiomeric excess of a portion of a scalemic sample. In addition, the possible contribution of the SDE phenomenon to the genesis of prebiotic homochirality is considered. Read More

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http://dx.doi.org/10.1007/s00726-019-02729-yDOI Listing

Computational characterization of structural and functional roles of DREB1A, DREB1B and DREB1C in enhancing cold tolerance in rice plant.

Amino Acids 2019 Mar 21. Epub 2019 Mar 21.

ICAR-National Rice Research Institute, Cuttack, Odisha, 753 006, India.

Rice serves as the major food for almost half of the world population. Because of its origin in the tropical and subtropical area, rice is more sensitive towards cold stress. Three homologs of DREB1, namely DREB1A, DREB1B and DREB1C are induced Queryduring cold stress and after binding with GCC-box in the promoter region of the target gene, they enhance cold tolerance in rice plants. Read More

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http://dx.doi.org/10.1007/s00726-019-02727-0DOI Listing

Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma.

Amino Acids 2019 Mar 21. Epub 2019 Mar 21.

The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041, Guangdong, People's Republic of China.

Lysyl oxidase-like 4 (LOXL4), a member of the LOX family proteins, catalyzes oxidative deamination of lysine residues in collagen and elastin, which are responsible for maintaining extracellular matrix homeostasis. In this study, the mRNA expression of LOXL4 in seven esophageal squamous cell carcinoma (ESCC) cell lines and 15 ESCC pairs of clinical samples were examined. Furthermore, LOXL4 protein levels in the ESCC cell lines were determined using western blotting. Read More

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http://dx.doi.org/10.1007/s00726-019-02723-4DOI Listing
March 2019
2 Reads

Silver cluster-amino acid interactions: a quantum-chemical study.

Amino Acids 2019 Mar 21. Epub 2019 Mar 21.

Saint-Petersburg State University, 199034, St. Petersburg, Russia.

Binding of silver ion (Ag) and two atomic neutral silver cluster (Ag) with a set of amino acids has been studied using Density Functional Theory (DFT) and ab initio MP2 method. We show that binding energy with Ag is higher for deprotonated anionic amino acids. Cysteine, aspartic acid, and tyrosine with deprotonated side chain exhibit the highest binding energy (G) values among all the amino acids: - 30. Read More

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http://dx.doi.org/10.1007/s00726-019-02728-zDOI Listing

Comparative stability of ficin and papain in acidic conditions and the presence of ethanol.

Amino Acids 2019 Mar 19. Epub 2019 Mar 19.

Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12 - 16, Belgrade, 11000, Republic of Serbia.

Proteolytic enzymes are used for proteolysis and peptide synthesis which can be run in various conditions including low pH value and the presence of ethanol. The most common cysteine protease applied in acidic-alcoholic conditions is well-characterized papain. Ficin, which is closely related to papain in terms of proteolytic activity and substrate specificity, could potentially be applied in the alcoholic beverage industry and peptide synthesis. Read More

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http://dx.doi.org/10.1007/s00726-019-02724-3DOI Listing

A novel comprehensive paradigm for the etiopathogenesis of multiple sclerosis: therapeutic approaches and future perspectives on its treatment.

Amino Acids 2019 Mar 18. Epub 2019 Mar 18.

Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185, Rome, Italy.

It is well recognized that variation in the geographical distribution of prevalence of multiple sclerosis (MS) exists: increasing the latitude its prevalence increases as well, but the underlying causes of such dissimilarity still remained elusive as of today. Currently, the most accredited hypothesis is that the closer to the equator the more pronounced is the amount of sunlight which, in turn, increases the production of vitamin D. Cholecalciferol is indeed deficient in MS patients, but this factor does not explain by itself the etiopathogenesis of the disease. Read More

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http://dx.doi.org/10.1007/s00726-019-02718-1DOI Listing
March 2019
2 Reads

Maternal L-proline supplementation during gestation alters amino acid and polyamine metabolism in the first generation female offspring of C57BL/6J mice.

Amino Acids 2019 Mar 16. Epub 2019 Mar 16.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China.

We recently reported that dietary supplementation with L-proline (proline) during gestation improved embryonic survival in C57BL/6J mice. The objective of the present study was to test the hypothesis that the effect of maternal proline supplementation on embryonic survival can be carried forward to the first generation female offspring. In the F0 generation, pregnant dams were fed a purified diet supplemented with 0 (control) or 5 g proline/kg diet. Read More

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http://dx.doi.org/10.1007/s00726-019-02717-2DOI Listing

Comparison between the effects of selenomethionine and S-adenosylmethionine in preventing cholestasis-induced rat liver damage.

Amino Acids 2019 Mar 16. Epub 2019 Mar 16.

Department of Biochemistry, Faculty of Medicine, University of Niš, Zorana Đinđića 81, 18000, Niš, Serbia.

We aimed to evaluate whether two methionine-related compounds, S-adenosylmethionine (SAM), and selenomethionine (SM), could lessen liver damage induced by regurgitated bile in a model of rat bile duct ligation (BDL). Hepatoprotective potentials of S-adenosylmethionine and selenomethionine were estimated based on the changes of serum liver damage parameters (aminotransferases, alkaline phosphatase, gamma-glutamyltranspeptidase and lactate dehydrogenase activity, and bilirubin concentration), tissue oxidative [xanthine oxidase (XO) and catalase activity, thiobarbituric acid reactive substances (TBARS) levels] and inflammatory [tumor necrosis factor-alfa (TNF-α) concentration] parameters, and morphological liver tissue alterations that follow cholestasis. The treatment regimens proved themselves able to prevent significant liver damage induced by cholestasis. Read More

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http://dx.doi.org/10.1007/s00726-019-02716-3DOI Listing

The additive effect of type 2 diabetes on fibrinogen, von Willebrand factor, tryptophan and threonine in people living with HIV.

Amino Acids 2019 Mar 13. Epub 2019 Mar 13.

School of Nursing, University of Texas, Austin, USA.

Chronic immune activation and ensuing inflammation that accompany HIV infection lead to adverse metabolic consequences and an increased risk of type 2 diabetes (T2D). We examined the additive effects of T2D on circulating biomarkers involved in inflammation, coagulation, and vascular function along with plasma amino acids in people living with HIV (PLWH). This cross-sectional study included PLWH with and without T2D (n = 32 total). Read More

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http://dx.doi.org/10.1007/s00726-019-02715-4DOI Listing

Carnosine's inhibitory effect on glioblastoma cell growth is independent of its cleavage.

Amino Acids 2019 Mar 12. Epub 2019 Mar 12.

Klinik Und Poliklinik für Neurochirurgie, Universitätsklinikum Leipzig AöR, Forschungslabore, Liebigstraße 19, 04103, Leipzig, Germany.

The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) inhibits the growth of tumor cells. As its component L-histidine mimics the effect, we investigated whether cleavage of carnosine is required for its antineoplastic effect. Using ten glioblastoma cell lines and cell cultures derived from 21 patients suffering from this malignant brain tumor, we determined cell viability under the influence of carnosine and L-histidine. Read More

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http://dx.doi.org/10.1007/s00726-019-02713-6DOI Listing
March 2019
3 Reads

Assessing acrolein for determination of the severity of brain stroke, dementia, renal failure, and Sjögren's syndrome.

Amino Acids 2019 Mar 12. Epub 2019 Mar 12.

Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.

It was found recently that acrolein (CH=CH-CHO), mainly produced from spermine, is more toxic than ROS (reactive oxygen species, O , HO, and ·OH). In this review, we describe how the seriousness of brain infarction, dementia, renal failure, and Sjӧgren's syndrome is correlated with acrolein. In brain infarction and dementia, it was possible to identify incipient patients with high sensitivity and specificity by measuring protein-conjugated acrolein (PC-Acro) in plasma together with IL-6 and CRP in brain infarction and Aβ in dementia. Read More

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http://dx.doi.org/10.1007/s00726-019-02700-xDOI Listing
March 2019
1 Read

Taurine supplementation in high-fat diet fed male mice attenuates endocrine pancreatic dysfunction in their male offspring.

Amino Acids 2019 Apr 4;51(4):727-738. Epub 2019 Mar 4.

Universidade Federal do Rio de Janeiro, Campus UFRJ-Macaé, Avenida São José do Barreto, 764, Macaé, RJ, CEP 27965-045, Brazil.

Obesity in fathers leads to DNA damage and epigenetic changes in sperm that may carry potential risk factors for metabolic diseases to the next generation. Taurine (TAU) supplementation has demonstrated benefits against testicular dysfunction and pancreatic islet impairments induced by obesity, but it is not known if these protective actions prevent the propagation of metabolic disruptions to the next generation; as such, we hypothesized that paternal obesity may increase the probability of endocrine pancreatic dysfunction in offspring, and that this could be prevented by TAU supplementation in male progenitors. To test this, male C57Bl/6 mice were fed on a control diet (CTL) or a high-fat diet (HFD) without or with 5% TAU in their drinking water (CTAU and HTAU) for 4 months. Read More

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http://dx.doi.org/10.1007/s00726-019-02712-7DOI Listing
April 2019
2 Reads

Elevated circulating asymmetric dimethylarginine levels in rheumatoid arthritis: a systematic review and meta-analysis.

Amino Acids 2019 Mar 4. Epub 2019 Mar 4.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, People's Republic of China.

Rheumatoid arthritis (RA) patients have increased risk of cardiovascular disease (CVD) death. Elevated asymmetric dimethylarginine (ADMA) levels have been reported to be an independent predictor of CVD morbidity and mortality, however, the role of ADMA in RA remains undetermined. To derive a more accurate estimation on circulating ADMA levels in RA patients, a meta-analysis was performed. Read More

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http://dx.doi.org/10.1007/s00726-019-02714-5DOI Listing

Crucial aminoacids in the F sector of the FF-ATP synthase address H across the inner mitochondrial membrane: molecular implications in mitochondrial dysfunctions.

Amino Acids 2019 Apr 23;51(4):579-587. Epub 2019 Feb 23.

Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy.

The eukaryotic FF-ATP synthase/hydrolase activity is coupled to H translocation through the inner mitochondrial membrane. According to a recent model, two asymmetric H half-channels in the a subunit translate a transmembrane vertical H flux into the rotor rotation required for ATP synthesis/hydrolysis. Along the H pathway, conserved aminoacid residues, mainly glutamate, address H both in the downhill and uphill transmembrane movements to synthesize or hydrolyze ATP, respectively. Read More

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http://dx.doi.org/10.1007/s00726-019-02710-9DOI Listing

Regulation of protein synthesis in porcine mammary epithelial cells by L-valine.

Amino Acids 2019 Apr 23;51(4):717-726. Epub 2019 Feb 23.

Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA.

This study was conducted to determine the catabolism of L-valine in porcine mammary epithelial cells (PMECs) and its role in stimulating protein synthesis in these cells. PMECs were incubated with 0.05-, 0. Read More

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http://dx.doi.org/10.1007/s00726-019-02709-2DOI Listing
April 2019
1 Read

Synthesis of selenopeptides: an alternative way of incorporating selenocystine.

Amino Acids 2019 Apr 23;51(4):661-667. Epub 2019 Feb 23.

Department of Chemistry, Howard University, 525 College Street NW, Washington, DC, 20059, USA.

Selenocysteine (Sec) residue cannot be directly attached to a peptide sequence unless the selenol form is protected beforehand and several problems have been reported in the preparation of Sec building blocks. In this article a series of selenocystine, the oxidized form of Sec, containing peptides has been synthesized using a new methodology, where Boc-NH-chloroalanine is coupled with methyl ester protected residues (Ala, Met, Phe) using DCC/HOBt as the coupling reagents providing di- and tripeptides. Further, the treatment of disodium diselenide with chloroalanine peptides (Boc-ClAla-Ala-OMe, Boc-ClAla-Met-OMe and Boc-ClAla-Ala-Phe-OMe) afforded the respective selenocystine-containing peptides (Boc-Sec-Ala-OMe, Boc-Sec-Met-OMe and Boc-Sec-Ala-Phe-OMe). Read More

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http://dx.doi.org/10.1007/s00726-019-02698-2DOI Listing

Correction to: Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery.

Amino Acids 2019 03;51(3):577

Department of Chemistry, Indiana-Purdue University, Fort Wayne, IN, 46805, USA.

The authors declare that the contributing author, Antonczak A.K., had no active academic address at or after the time of publication. Read More

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http://dx.doi.org/10.1007/s00726-019-02703-8DOI Listing

Isolation and characterization of collagen type I crosslink from skin: high-resolution NMR reveals diastereomers of hydroxylysinonorleucine crosslink.

Amino Acids 2019 Apr 20;51(4):705-715. Epub 2019 Feb 20.

Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.

Skin is made up of mainly collagen type I and its structure is stabilised by the formation of covalent immature and mature crosslinks. In this study, collagen immature crosslink hydroxylysinonorleucine (HLNL) was isolated from bovine skin in high purity using two sequential purification steps. These consisted of preparative fibrous cellulose and size exclusion chromatography. Read More

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http://dx.doi.org/10.1007/s00726-019-02708-3DOI Listing

The effects of collagen peptides on muscle damage, inflammation and bone turnover following exercise: a randomized, controlled trial.

Amino Acids 2019 Apr 19;51(4):691-704. Epub 2019 Feb 19.

Institute of Cellular Medicine, Faculty of Medical Sciences, School of Biomedicine, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.

This study examined whether consuming collagen peptides (CP) before and after strenuous exercise alters markers of muscle damage, inflammation and bone turnover. Using a double-blind, independent group's design, 24 recreationally active males consumed either 20 g day of CP or a placebo control (CON) for 7 days before and 2 days after performing 150 drop jumps. Maximal isometric voluntary contractions, countermovement jumps (CMJ), muscle soreness (200 mm visual analogue scale), pressure pain threshold, Brief Assessment of Mood Adapted (BAM +) and a range of blood markers associated with muscle damage, inflammation and bone turnover C-terminal telopeptide of type 1 collagen (β-CTX) and N-terminal propeptides of type 1 pro-collagen (P1NP) were measured before supplementation (baseline; BL), pre, post, 1. Read More

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http://dx.doi.org/10.1007/s00726-019-02706-5DOI Listing

α/β-Chimera peptide synthesis with cyclic β-sugar amino acids: the efficient coupling protocol.

Amino Acids 2019 Apr 13;51(4):669-678. Epub 2019 Feb 13.

Laboratory of Structural Chemistry and Biology, Institute of Chemistry, ELTE Eötvös Loránd University, Pázmány P. Stny. 1/A, Budapest, 1117, Hungary.

The synthesis of α/β-chimeras comprises peptide bond formation from α- to β-, from β- to β-, and from β- to α-amino acid residues. The fine-tuned solid phase synthesis of -GXXG- chimera peptides containing the simplest achiral α-amino acid glycine and two cyclic SAAs of different ring size [X denoting cyclic β-Sugar Amino Acids (β-SAA)] is reported, variants containing Fmoc-RibAFU(ip)-OH a furanoid-, and Fmoc-GlcAPU(Me)-OH a pyranoid-type structural "Lego-element". Systematic search for the best coupling strategy with both H-β-SAA-OHs is described, including the comparison of the different coupling reagents and conditions. Read More

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http://dx.doi.org/10.1007/s00726-019-02702-9DOI Listing
April 2019
1 Read

Study of cross talk between phosphatases and OGA on a ZO-3-derived peptide.

Amino Acids 2019 Apr 6;51(4):739-743. Epub 2019 Feb 6.

Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB, Utrecht, The Netherlands.

O-GlcNAcylation, like phosphorylation, is a dynamic and rapid posttranslational modification which regulates many cellular processes. Phosphorylation on tyrosine and O-GlcNAcylation on nearby serine or threonine residues may modulate each other. Indeed, by using a microarray with a peptide model system based on the ZO-3 protein, extensive cross talk between O-GlcNAcylation by OGT and phosphorylation by kinases was observed. Read More

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http://dx.doi.org/10.1007/s00726-019-02699-1DOI Listing

Amino acid profiling as a method of discovering biomarkers for diagnosis of neurodegenerative diseases.

Amino Acids 2019 Mar 6;51(3):367-371. Epub 2019 Feb 6.

Department of Toxicology, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland.

The trend of growing population of 60+ years old people is visible in most of the highly developed European countries. Recently researchers have shown an increased interest in aging-associated diseases including neurological disorders. Neurodegenerative diseases are a very important clinical problem for several reasons. Read More

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http://dx.doi.org/10.1007/s00726-019-02705-6DOI Listing
March 2019
2 Reads

Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing.

Amino Acids 2019 Apr 6;51(4):679-690. Epub 2019 Feb 6.

Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis-Viale dell'Università 16, 35020, Legnaro, PD, Italy.

The preservation of enzymatic activity is a fundamental requirement for exploiting hybrid nano-bio-conjugates, and the control over protein-nanoparticle interactions, leading to stable and catalytically active hybrids, represents the key for designing new biosensing platforms. In this scenario, surface active maghemite nanoparticles (SAMNs) represent a new class of naked magnetic nanoparticles, displaying peculiar electrocatalytic features and the ability to selectively bind proteins. Recombinant aminoaldehyde dehydrogenase from tomato (SlAMADH1) was used as a model protein, and successfully immobilized by self-assembly on the surface of naked SAMNs, where its enzymatic activity resulted preserved for more than 6 months. Read More

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http://dx.doi.org/10.1007/s00726-019-02704-7DOI Listing

Correction to: An update on carnosine and anserine research.

Amino Acids 2019 01;51(1)

Department of Medicine, Envirome Institute, Diabetes and Obesity Center, University of Louisville, Louisville, KY, USA.

The original version of this article unfortunately contained a mistake. The author "Shahid Baba" would like to include the middle name "P" in the online published article. Read More

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http://link.springer.com/10.1007/s00726-019-02701-w
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http://dx.doi.org/10.1007/s00726-019-02701-wDOI Listing
January 2019
8 Reads

Low-resolution structure, oligomerization and its role on the enzymatic activity of a sucrose-6-phosphate hydrolase from Bacillus licheniformis.

Amino Acids 2019 Apr 23;51(4):599-610. Epub 2019 Jan 23.

Sao Carlos Institute of Physics (IFSC), University of Sao Paulo (USP), Avenida João Dagnone, no 1100, Jardim Santa Angelina, São Carlos, SP, 13563-120, Brazil.

Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. Read More

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http://dx.doi.org/10.1007/s00726-018-02690-2DOI Listing
April 2019
4 Reads

Safety assessment of L-lysine oral intake: a systematic review.

Amino Acids 2019 Apr 19;51(4):647-659. Epub 2019 Jan 19.

Laboratory of Food Chemistry, Yokohama University of Pharmacy, 601 Matano-cho, Totsuka-ku, Yokohama, Kanagawa, 245-0066, Japan.

Currently, the use of amino acids in supplements and functional foods is increasing globally. However, there are no guidelines for the upper limit of ingestion for the safe use of these amino acids. Safety evaluation of chemical substances is generally performed through non-clinical and clinical studies. Read More

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http://dx.doi.org/10.1007/s00726-019-02697-3DOI Listing
April 2019
1 Read

Taurine protects against NMDA-induced retinal damage by reducing retinal oxidative stress.

Amino Acids 2019 Apr 17;51(4):641-646. Epub 2019 Jan 17.

Faculty of Medicine, International Medical University, IMU Clinical School, Seremban, Malaysia.

This study aimed to evaluate effect of TAU on NMDA-induced changes in retinal redox status, retinal cell apoptosis and retinal morphology in Sprague-Dawley rats. Taurine was injected intravitreally as pre-, co- or post-treatment with NMDA and 7 days post-treatment retinae were processed for estimation of oxidative stress, retinal morphology using H&E staining and retinal cell apoptosis using TUNEL staining. Treatment with TAU, particularly pre-treatment, significantly increased retinal glutathione, superoxide dismutase and catalase levels compared to NMDA-treated rats; whereas, the levels of malondialdehyde reduced significantly. Read More

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http://dx.doi.org/10.1007/s00726-019-02696-4DOI Listing
April 2019
3.293 Impact Factor

Effects of beta-alanine supplementation on muscle function during recovery from resistance exercise in young adults.

Amino Acids 2019 Apr 9;51(4):589-597. Epub 2019 Jan 9.

Center of Research in Health Sciences, North University of Paraná (UNOPAR), Avenue Paris, 675, Jardim Piza, Londrina, PR, CEP: 86041-120, Brazil.

β-Alanine supplementation has been shown to increase muscle carnosine levels and exercise performance. However, its effects on muscle recovery from resistance exercise (RE) remains unknown. The purpose of this study was to investigate the effects of β-alanine supplementation on muscle function during recovery from a single session of high-intensity RE. Read More

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http://dx.doi.org/10.1007/s00726-018-02686-yDOI Listing
April 2019
1 Read

An update on carnosine and anserine research.

Amino Acids 2019 Jan 7;51(1):1-4. Epub 2019 Jan 7.

Department of Medicine, Envirome Institute, Diabetes and Obesity Center, University of Louisville, Louisville, KY, USA.

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http://dx.doi.org/10.1007/s00726-018-02689-9DOI Listing
January 2019
9 Reads

GC-MS measurement of biological N-hydroxy-L-arginine, a stepmotherly investigated endogenous nitric oxide synthase substrate and arginase inhibitor.

Amino Acids 2019 Apr 4;51(4):627-640. Epub 2019 Jan 4.

Institute of Toxicology, Core Unit Proteomics, Hannover Medical School, Carl-Neuberg-Strasse 1, 30623, Hannover, Germany.

L-Arginine is converted by nitric oxide synthase (NOS) to L-citrulline and nitric oxide (NO). N-Hydroxy-L-arginine (NOHA) is the isolable intermediate of this reaction. NOHA has been identified in biological samples by gas chromatography-mass spectrometry (GC-MS) and quantified by high-performance liquid chromatography (HPLC). Read More

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http://dx.doi.org/10.1007/s00726-018-02695-xDOI Listing

Effect of renal function on homeostasis of asymmetric dimethylarginine (ADMA): studies in donors and recipients of renal transplants.

Amino Acids 2019 Mar 4;51(3):565-575. Epub 2019 Jan 4.

Division of Nephrology, Department of Internal Medicine, University Medical Center Groningen, Sector A, University of Groningen, PO Box 30.001, 9700 RB, Groningen, The Netherlands.

Asymmetric dimethylarginine (ADMA) is a methylated form of arginine and an endogenous nitric oxide synthase inhibitor. Renal function decline is associated with increase of plasma ADMA in chronic kidney disease populations. It is yet unknown how isolated renal function impairment affects ADMA homeostasis in healthy humans. Read More

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http://dx.doi.org/10.1007/s00726-018-02693-zDOI Listing

Carnosinase concentration, activity, and CNDP1 genotype in patients with type 2 diabetes with and without nephropathy.

Amino Acids 2019 Apr 4;51(4):611-617. Epub 2019 Jan 4.

Vth Department of Medicine (Nephrology/Endocrinology/Rheumatology) University Medical Center Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.

This study assessed if serum carnosinase (CNDP1) activity and concentration in patients with type 2 diabetes mellitus (T2D) with diabetic nephropathy (DN) differs from those without nephropathy. In a cross-sectional design 127 patients with T2D with DN ((CTG) homozygous patients n = 45) and 145 patients with T2D without nephropathy ((CTG) homozygous patients n = 47) were recruited. Univariate and multivariate regression analyses were performed to predict factors relevant for serum CNDP1 concentration. Read More

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http://dx.doi.org/10.1007/s00726-018-02692-0DOI Listing
April 2019
12 Reads

Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5.

Amino Acids 2019 Apr 2;51(4):619-626. Epub 2019 Jan 2.

Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.

The insulin superfamily is a group of homologous proteins that are further divided into the insulin family and relaxin family according to their distinct receptors. All insulin superfamily members contain three absolutely conserved disulfide linkages and a nonchiral Gly residue immediately following the first B-chain cysteine. The functionality of this conserved Gly residue in the insulin family has been studied by replacing it with natural L-amino acids or the corresponding unnatural D-amino acids. Read More

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http://dx.doi.org/10.1007/s00726-018-02694-yDOI Listing

Altered brain arginine metabolism in a mouse model of tauopathy.

Amino Acids 2019 Mar 2;51(3):513-528. Epub 2019 Jan 2.

Department of Anatomy, School of Biomedical Sciences, Brain Health Research Centre, University of Otago, Dunedin, New Zealand.

Tauopathies consist of intracellular accumulation of hyperphosphorylated and aggregated microtubule protein tau, which remains a histopathological feature of Alzheimer's disease (AD) and frontotemporal dementia. L-Arginine is a semi-essential amino acid with a number of bioactive molecules. Its downstream metabolites putrescine, spermidine, and spermine (polyamines) are critically involved in microtubule assembly and stabilization. Read More

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http://link.springer.com/10.1007/s00726-018-02687-x
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http://dx.doi.org/10.1007/s00726-018-02687-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431576PMC
March 2019
14 Reads
3.293 Impact Factor

Synthesis, characterization and anticonvulsant activity of new azobenzene-containing VV-hemorphin-5 bio photoswitch.

Amino Acids 2019 Mar 2;51(3):549-563. Epub 2019 Jan 2.

Department of Organic Chemistry, University of Chemical Technology and Metallurgy, 1756, Sofia, Bulgaria.

A novel analog of VV-hemorphin-5 containing azobenzene moiety has been synthesized and investigated for anticonvulsant activity in relation to its E → Z photophysical properties activated by long wavelength light at 365 nm. The synthesis was achieved by a modified SPPS by Fmoc-dimerization strategy. The electrochemical behavior before and after UV illumination was investigated using different voltammetric modes. Read More

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http://dx.doi.org/10.1007/s00726-018-02691-1DOI Listing

Development and validation of GC-MS methods for the comprehensive analysis of amino acids in plasma and urine and applications to the HELLP syndrome and pediatric kidney transplantation: evidence of altered methylation, transamidination, and arginase activity.

Amino Acids 2019 Mar 2;51(3):529-547. Epub 2019 Jan 2.

Core Unit Proteomics, Hannover Medical School, Institute of Toxicology, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

We developed and validated gas chromatography-mass spectrometry (GC-MS) methods for the simultaneous measurement of amino acids and their metabolites in 10-µL aliquots of human plasma and urine. De novo synthesized trideutero-methyl esters were used as internal standards. Plasma proteins were precipitated by acidified methanol and removed by centrifugation. Read More

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http://dx.doi.org/10.1007/s00726-018-02688-wDOI Listing

Structural properties and role of the endocannabinoid lipases ABHD6 and ABHD12 in lipid signalling and disease.

Amino Acids 2019 Feb 18;51(2):151-174. Epub 2018 Dec 18.

Department of Biomedicine, University of Bergen, Bergen, Norway.

The endocannabinoid (eCB) system is an important part of both the human central nervous system (CNS) and peripheral tissues. It is involved in the regulation of various physiological and neuronal processes and has been associated with various diseases. The eCB system is a complex network composed of receptor molecules, their cannabinoid ligands, and enzymes regulating the synthesis, release, uptake, and degradation of the signalling molecules. Read More

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http://link.springer.com/10.1007/s00726-018-2682-8
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http://dx.doi.org/10.1007/s00726-018-2682-8DOI Listing
February 2019
9 Reads

Pharmacological activation of dimethylarginine dimethylaminohydrolase (DDAH) activity by inorganic nitrate and DDAH inhibition by N-hydroxy-L-arginine, N,N-dimethyl-L-citrulline and N,N-dimethyl-N-hydroxy-L-citrulline: results and overview.

Amino Acids 2019 Mar 8;51(3):483-494. Epub 2018 Dec 8.

Institute of Toxicology, Core Unit Proteomics, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are endogenous inhibitors of nitric oxide (NO) synthase. SDMA is excreted in the urine without major metabolization. About 10% of daily produced ADMA are excreted unchanged in the urine. Read More

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http://dx.doi.org/10.1007/s00726-018-2684-6DOI Listing
March 2019
2 Reads

The first comprehensive description of the expression profile of genes involved in differential body growth and the immune system of the Jeju Native Pig and miniature pig.

Amino Acids 2019 Mar 5;51(3):495-511. Epub 2018 Dec 5.

Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690756, Jeju-Do, Republic of Korea.

Sus scrofa provides a major source of animal protein for humans as well as being an excellent biomedical model. This study was carried out to understand, in detail, the genetic and functional variants of Jeju Native Pigs and miniature pigs through differential expression profiling of the genes controlling their immune response, growth performance, and meat quality. The Illumina HiSeq 2000 platform was used for generating 1. Read More

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http://dx.doi.org/10.1007/s00726-018-2685-5DOI Listing
March 2019
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Globular protein backbone conformational disorder in crystal structures.

Authors:
Oliviero Carugo

Amino Acids 2019 Mar 4;51(3):475-481. Epub 2018 Dec 4.

Department of Chemistry, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.

Proteins are not static molecules but dynamic entities able to modify their structure for several reasons, from the necessity to recognize partners to the regulation of their thermodynamic stability. Conformational disorder is frequent in protein structures and atoms can have, in protein crystal structures, two or more alternative, equilibrium positions close to each other. Here, a set of protein crystal structures refined at very high resolution (1 Å or better) is examined to characterize the conformational disorder of the backbone atoms, which is not infrequent: about 15% of the protein backbone atoms are conformationally disordered and three quarters of them have been deposited with two or more equilibrium positions (most of the others were not detected in the electron density maps). Read More

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http://dx.doi.org/10.1007/s00726-018-2683-7DOI Listing

Glycine supplementation to breast-fed piglets attenuates post-weaning jejunal epithelial apoptosis: a functional role of CHOP signaling.

Amino Acids 2019 Mar 28;51(3):463-473. Epub 2018 Nov 28.

Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China.

This study was conducted to test the hypothesis that preweaning  glycine supplementation to breast-fed piglets alleviated the post-weaning  apoptosis of jejunal epithelium through CHOP signaling. Seven-day-old sow-reared piglets were orally administrated with 0, 50, 100, or 200% of glycine intake from sow's milk twice daily for 14 days and then were weaned at 21 days of age. Tissue samples were collected at 28 days of age for determining intestinal morphology, serum diamine oxidase (DAO) activity, abundances of proteins involved in ER stress and apoptosis. Read More

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http://dx.doi.org/10.1007/s00726-018-2681-9DOI Listing
March 2019
1 Read
3.293 Impact Factor

Carnosine protects cardiac myocytes against lipid peroxidation products.

Amino Acids 2019 Jan 17;51(1):123-138. Epub 2018 Nov 17.

Department of Medicine, Diabetes and Obesity Center, University of Louisville, 580 South Preston Street, Delia Baxter Building, Room 421A, Louisville, KY, 40202, USA.

Endogenous histidyl dipeptides such as carnosine (β-alanine-L-histidine) form conjugates with lipid peroxidation products such as 4-hydroxy-trans-2-nonenal (HNE and acrolein), chelate metals, and protect against myocardial ischemic injury. Nevertheless, it is unclear whether these peptides protect against cardiac injury by directly reacting with lipid peroxidation products. Hence, to examine whether changes in the structure of carnosine could affect its aldehyde reactivity and metal chelating ability, we synthesized methylated analogs of carnosine, balenine (β-alanine-N-methylhistidine) and dimethyl balenine (DMB), and measured their aldehyde reactivity and metal chelating properties. Read More

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http://link.springer.com/10.1007/s00726-018-2676-6
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http://dx.doi.org/10.1007/s00726-018-2676-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377314PMC
January 2019
11 Reads
3.293 Impact Factor