4,192 results match your criteria Amino acids[Journal]


Epibrassinolide-induced autophagy occurs in an Atg5-independent manner due to endoplasmic stress induction in MEF cells.

Amino Acids 2020 May 24. Epub 2020 May 24.

Department of Molecular Biology and Genetics, Istanbul Kultur University, Ataköy Campus, Bakirkoy, 34156, Istanbul, Turkey.

Epibrassinolide (EBR), a polyhydroxysteroid belongs to plant growth regulator family, brassinosteroids and has been shown to have a similar chemical structure to mammalian steroid hormones. Our findings indicated that EBR could trigger apoptosis in cancer cells via induction of endoplasmic reticulum (ER) stress, caused by protein folding disturbance in the ER. Normal cells exhibited a remarkable resistance to EBR treatment and avoid from apoptotic cell death. Read More

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http://dx.doi.org/10.1007/s00726-020-02857-wDOI Listing

Glycine metabolomic changes induced by anticancer agents in A549 cells.

Amino Acids 2020 May 19;52(5):793-809. Epub 2020 May 19.

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen, 361102, China.

Glycine plays a key role in rapidly proliferating cancer cells such as A549 cells. Targeting glycine metabolism is considered as a potential means for cancer treatment. However, the drug-induced alterations in glycine metabolism have not yet been investigated. Read More

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http://dx.doi.org/10.1007/s00726-020-02853-0DOI Listing

Biological macromolecule binding and anticancer activity of synthetic alkyne-containing L-phenylalanine derivatives.

Amino Acids 2020 May 19;52(5):755-769. Epub 2020 May 19.

Istituto di Biostrutture e Bioimmagini, IBB-CNR, Via Mezzocannone 16, 80134, Naples, Italy.

Herein, we described the synthesis of two L-phenylalanines α-derivatized with a terminal alkyne moiety whose structures differed by phenyl ring halogen substitution (two o-Cl in 1 vs. one p-Br in 2) and investigated their effect on biological macromolecules and living cells. We explored their interaction with quadruplex DNA (G4 DNA), using tel and c-myc as models, and bovine serum albumin (BSA). Read More

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http://dx.doi.org/10.1007/s00726-020-02849-wDOI Listing

Design of BRC analogous peptides based on the complex BRC8-RAD51 and the preliminary study on the peptide structures.

Amino Acids 2020 May 16;52(5):831-839. Epub 2020 May 16.

School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.

BRCA2 is an important tumor suppressor gene that plays a critical role in preserving the stability of cellular genetic information, participating in DNA repair by engaging in binding interactions with RAD51 proteins. However, the lack of structural data on BRCA2 and RAD51 makes the study of their interaction mechanism still a great challenge. We characterize the structure of the BRC8-RAD51 complex using ZDOCK protein docking software and identify the potential non-conserved active site of BRC8 via virtual alanine scanning, utilizing the obtained results to synthesize BRC8, its six analogous peptides (BRC8-1 to BRC8-6), and critical peptide fragment of RAD51 (RAD51(231-260)) by Fmoc solid-phase synthesis. Read More

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http://dx.doi.org/10.1007/s00726-020-02856-xDOI Listing

Nutrition and metabolism of glutamate and glutamine in fish.

Amino Acids 2020 May 13;52(5):671-691. Epub 2020 May 13.

Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA.

Glutamate (Glu) and glutamine (Gln) comprise a large proportion of total amino acids (AAs) in fish in the free and protein-bound forms. Both Glu and Gln are synthesized de novo from other α-amino acids and ammonia. Although these two AAs had long been considered as nutritionally non-essential AAs for an aquatic animal, they must be included adequately in its diet to support optimal health (particularly intestinal health) and maximal growth. Read More

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http://dx.doi.org/10.1007/s00726-020-02851-2DOI Listing

Increased plasma level of apelin with NYHA grade II and III but not IV.

Amino Acids 2020 May 9;52(5):823-829. Epub 2020 May 9.

Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Haidian District, Beijing, 100191, China.

The change in plasma apelin level in heart failure (HF) patients is controversial. We investigated the change in plasma apelin level in HF patients versus control and non-HF patients. The plasma level of apelin was measured by ELISA and plasma level of B-type natriuretic peptide (BNP) by fluorescence immunoassay. Read More

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http://dx.doi.org/10.1007/s00726-020-02855-yDOI Listing

The kinetics and mechanisms of reactions in the flow systems glycine-sodium trimetaphosphate-imidazoles: the crucial role of imidazoles in prebiotic peptide syntheses.

Amino Acids 2020 May 5;52(5):811-821. Epub 2020 May 5.

A. M. Butlerov Chemistry Institute, Kazan Federal University, Kremlevskaya st. 18, 420008, Kazan, Russian Federation.

The kinetics of oligopeptides formation in the flow systems glycine-sodium trimetaphosphate-imidazole/N-methylimidazole at thermocyclic regime has been investigated by HPLC and P NMR methods in the ranges of temperature from 45 to 90 °C and pH from 8.5 to 11.5. Read More

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http://dx.doi.org/10.1007/s00726-020-02854-zDOI Listing

Integrating RNA-sequencing and untargeted LC-MS metabolomics to evaluate the effect of lysine deficiency on hepatic functions in Holstein calves.

Amino Acids 2020 May 5;52(5):781-792. Epub 2020 May 5.

Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Sino-US Joint Lab On Nutrition and Metabolism of Ruminants, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Lysine (Lys) is majorly metabolized in the liver. The liver functional consequences of a dietary Lys deficiency in young Holstein calves are unknown. This study aimed to investigate the effects of Lys deficiency in Holstein calf livers using RNA-sequencing and untargeted LC-MS metabolomics. Read More

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http://dx.doi.org/10.1007/s00726-020-02852-1DOI Listing
May 2020
3.293 Impact Factor

Enhancing effect of macroporous adsorption resin on gamma-aminobutyric acid production by Enterococcus faecium in whole-cell biotransformation system.

Amino Acids 2020 May 5;52(5):771-780. Epub 2020 May 5.

College of Chemistry and Chemical Engineering, Lingnan Normal University, Cunjin Road 29, Chikan District, Zhanjiang, 524048, Guangdong, China.

Gamma-aminobutyric acid (GABA) biosynthesis depended to a great extent on the biotransformation characterization of glutamate decarboxylase (GAD) and process conditions. In this paper, the enhancing effect of D101 macroporous adsorption resin (MAR) on the GABA production was investigated based on the whole-cell biotransformation characterization of Enterococcus faecium and adsorption characteristics of D101 MAR. The results indicated that the optimal pH for reaction activity of whole-cell GAD and pure GAD was 4. Read More

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http://dx.doi.org/10.1007/s00726-020-02850-3DOI Listing

Inhibition of EIF-5A prevents apoptosis in human cardiomyocytes after malaria infection.

Amino Acids 2020 May 4;52(5):693-710. Epub 2020 May 4.

Astel Diagnostics, Kampala, Uganda.

In this study, a determination of Troponin I and creatine kinase activity in whole-blood samples in a cohort of 100 small infants in the age of 2-5 years from Uganda with complicated Plasmodium falciparum malaria suggests the prevalence of cardiac symptoms in comparison to non-infected, healthy patients. Troponin I and creatine kinase activity increased during infection. Different reports showed that complicated malaria coincides with hypoxia in children. Read More

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http://dx.doi.org/10.1007/s00726-020-02843-2DOI Listing

Comparing activity, toxicity and model membrane interactions of Jelleine-I and Trp/Arg analogs: analysis of peptide aggregation.

Amino Acids 2020 May 4;52(5):725-741. Epub 2020 May 4.

Departamento de Física, Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas (Ibilce), R. Cristóvão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brazil.

Increasing resistance in antibiotic and chemotherapeutic treatments has been pushing studies of design and evaluation of bioactive peptides. Designing relies on different approaches from minimalist sequences and endogenous peptides modifications to computational libraries. Evaluation relies on microbiological tests. Read More

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http://dx.doi.org/10.1007/s00726-020-02847-yDOI Listing

Dietary GABA induces endogenous synthesis of a novel imidazole peptide homocarnosine in mouse skeletal muscles.

Amino Acids 2020 May 2;52(5):743-753. Epub 2020 May 2.

Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima City, 1-4-4 Kagamiyama, Hiroshima, 739-8528, Japan.

Carnosine (β-alanyl-L-histidine) is an imidazole dipeptide present at high concentrations in skeletal muscles, where it plays a beneficial role. However, oral intake of carnosine or β-alanine to increase skeletal muscle carnosine levels has disadvantages such as low efficiency and side effects. Therefore, we proposed homocarnosine (γ-aminobutyryl-L-histidine) as a novel alternative imidazole peptide for skeletal muscle based on its structural similarity to carnosine. Read More

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http://dx.doi.org/10.1007/s00726-020-02848-xDOI Listing

Microwave-assisted cleavage of cysteine perfluoroaryl thioethers.

Amino Acids 2020 May 29;52(5):841-845. Epub 2020 Apr 29.

Department of Chemistry, Dickinson College, 28 North College Street, Carlisle, PA, 17013, USA.

The cysteine- perfluoroarene SAr reaction allows for the sequence-specific attachment of dyes and affinity tags to peptides and proteins. However, while many methods exist for the desulfuration of native and functionalized cysteine residues, there are no reports of their application to perfluoroarylated cysteines. Herein we report both the hydrogenolysis of a perfluoroarylated cysteine to alanine and elimination to dehydroalanine, reactions that are both accelerated by microwave irradiation. Read More

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http://dx.doi.org/10.1007/s00726-020-02846-zDOI Listing

Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues.

Amino Acids 2020 May 21;52(5):711-724. Epub 2020 Apr 21.

School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.

Erythrocytes have a well-defined role in the gaseous exchange of oxygen and carbon dioxide in the mammalian body. The erythrocytes can contain more than half of the free amino acids present in whole blood. Based on measures showing that venous erythrocyte levels of amino acids are much less than arterial erythrocyte levels, it has previously been proposed that erythrocytes also play a role in the delivery of amino acids to tissues in the body. Read More

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http://dx.doi.org/10.1007/s00726-020-02845-0DOI Listing

The lineage and diversity of putative amino acid sensor ACR proteins in plants.

Amino Acids 2020 Apr 18;52(4):649-666. Epub 2020 Apr 18.

Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.

Amino acid metabolic enzymes often contain a regulatory ACT domain, named for aspartate kinase, chorismate mutase, and TyrA (prephenate dehydrogenase). Arabidopsis encodes 12 putative amino acid sensor ACT repeat (ACR) proteins, all containing ACT repeats but no identifiable catalytic domain. Arabidopsis ACRs comprise three groups based on domain composition and sequence: group I and II ACRs contain four ACTs each, and group III ACRs contain two ACTs. Read More

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http://dx.doi.org/10.1007/s00726-020-02844-1DOI Listing

Nitric oxide-related markers link inversely to blood pressure in black boys and men: the ASOS and African-PREDICT studies.

Amino Acids 2020 Apr 18;52(4):639-648. Epub 2020 Apr 18.

Hypertension in Africa Research Team (HART), North-West University, Potchefstroom, South Africa.

Nitric oxide plays an important role in maintaining endothelial function, while increased oxidative stress may lead to nitric oxide inactivation and cardiovascular disease. If nitric oxide biosynthesis/bioavailability is already suppressed early in life, it may potentially predispose an individual to the early development of cardiovascular disease. We therefore aimed to identify differences in nitric oxide-related markers (urinary nitrate, nitrite and the nitrate-to-nitrite ratio (UR)) between young black and white individuals, and whether these markers are associated with blood pressure and carotid intima media thickness. Read More

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http://dx.doi.org/10.1007/s00726-020-02842-3DOI Listing
April 2020
3.293 Impact Factor

Characterization of macrophage phenotype, redox, and purinergic response upon chronic treatment with methionine and methionine sulfoxide in mice.

Amino Acids 2020 Apr 3;52(4):629-638. Epub 2020 Apr 3.

Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos (CCQFA), Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Pelotas, RS, 96160-000, Brasil.

Hypermethioninemia is a disorder characterized by high plasma levels of methionine (Met) and its metabolites such as methionine sulfoxide (MetO). Studies have reported associated inflammatory complications, but the mechanisms involved in the pathophysiology of hypermethioninemia are still uncertain. The present study aims to evaluate the effect of chronic administration of Met and/or MetO on phenotypic characteristics of macrophages, in addition to oxidative stress, purinergic system, and inflammatory mediators in macrophages. Read More

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http://dx.doi.org/10.1007/s00726-020-02841-4DOI Listing

Doublecortin and IGF-1R protein levels are reduced in spite of unchanged DNA methylation in the hippocampus of aged rats.

Amino Acids 2020 Apr 1;52(4):543-553. Epub 2020 Apr 1.

Paracelsus Private Medical University, 5020, Salzburg, Austria.

The aim of the current study was to investigate whether doublecortin (DCX), insulin-like growth factor receptor 1 (IGF-1R) and metabotropic glutamate receptor 5 (mGluR5) levels are indeed modified in the aging rat hippocampal individual subareas (rather than total hippocampal tissue as in previous reports) at the protein and mRNA level and whether the methylation status contributes to these changes. Since the aging population is not homogeneous in terms of spatial memory performance, we examined whether changes in DCX, IGF-1R and mGluR5 are linked to cognitive aging. Aged (22 months) male Sprague Dawley rats were trained in the hole-board, a spatial memory task, and were subdivided according to performance to aged impaired and aged unimpaired groups. Read More

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http://dx.doi.org/10.1007/s00726-020-02834-3DOI Listing

The intracellular domain of BP180/collagen XVII is intrinsically disordered and partially folds in an anionic membrane lipid-mimicking environment.

Amino Acids 2020 Apr 27;52(4):619-627. Epub 2020 Mar 27.

PEDEGO Research Unit, Department of Dermatology, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland.

The trimeric transmembrane collagen BP180, also known as collagen XVII, is an essential component of hemidesmosomes at the dermal-epidermal junction and connects the cytoplasmic keratin network to the extracellular basement membrane. Dysfunction of BP180 caused by mutations in patients with junctional epidermolysis bullosa or autoantibodies in those with bullous pemphigoid leads to severe skin blistering. The extracellular collagenous domain of BP180 participates in the protein's triple-helical folding, but the structure and functional importance of the intracellular domain (ICD) of BP180 are largely unknown. Read More

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http://dx.doi.org/10.1007/s00726-020-02840-5DOI Listing

Potential anticonvulsant activity of novel VV-hemorphin-7 analogues containing unnatural amino acids: synthesis and characterization.

Amino Acids 2020 Apr 24;52(4):567-585. Epub 2020 Mar 24.

Department of Analytical Chemistry, University of Chemical Technology and Metallurgy, 1756, Sofia, Bulgaria.

Herein, some new analogues of VV-hemorphin-7, modified at position 4 and 7 by the unnatural amino acids followed the structure Val-Val-Tyr-Xxx-Trp-Thr-Yyy-Arg-Phe-NH, where Xxx is Ac5c (1-aminocyclopentanecarboxylic acid) or Ac6c (1-aminocyclohexane carboxylic acid) and Yyy is Dap (diaminopropanoic acid) or Dab (diaminobutanoic acid), were synthesized, characterized and investigated for anticonvulsant activity. The new synthetic peptide analogues were prepared by standard solid-phase peptide synthesis-Fmoc chemistry. A single intracerebroventricular (i. Read More

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http://dx.doi.org/10.1007/s00726-020-02836-1DOI Listing

Systematic profiling of staralog response to acquired drug resistant kinase gatekeeper mutations in targeted cancer therapy.

Amino Acids 2020 Apr 23;52(4):511-521. Epub 2020 Mar 23.

College of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, China.

Kinase-targeted therapy has been widely used as a lifesaving strategy for cancer patients. However, many patients treated with targeted cancer drugs are clinically observed to rapidly develop acquired resistance. Kinase gatekeeper mutation is one of the most chief factors contributing to the resistance, which modulates the accessibility of kinase's ATP-binding pocket. Read More

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http://dx.doi.org/10.1007/s00726-020-02832-5DOI Listing

Prenatal expression of D-aspartate oxidase causes early cerebral D-aspartate depletion and influences brain morphology and cognitive functions at adulthood.

Amino Acids 2020 Apr 17;52(4):597-617. Epub 2020 Mar 17.

CEINGE Biotecnologie Avanzate, 80145, Naples, Italy.

The free D-amino acid, D-aspartate, is abundant in the embryonic brain but significantly decreases after birth. Besides its intracellular occurrence, D-aspartate is also present at extracellular level and acts as an endogenous agonist for NMDA and mGlu5 receptors. These findings suggest that D-aspartate is a candidate signaling molecule involved in neural development, influencing brain morphology and behaviors at adulthood. Read More

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http://dx.doi.org/10.1007/s00726-020-02839-yDOI Listing
April 2020
3.293 Impact Factor

Presence of guanidinoacetate may compensate creatine absence and account for less statin-induced muscle damage in GAMT-deficient compared to AGAT-deficient mice.

Amino Acids 2020 Apr 14;52(4):667-669. Epub 2020 Mar 14.

Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Mother and Child Sciences (DINOGMI), University of Genoa, Genoa, Italy.

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http://dx.doi.org/10.1007/s00726-020-02838-zDOI Listing

Effects of maternal L-proline supplementation on inflammatory cytokines at the placenta and fetus interface of mice.

Amino Acids 2020 Apr 14;52(4):587-596. Epub 2020 Mar 14.

Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China.

Dietary L-proline (proline) supplementation during gestation enhances fetal survival and placental development in mice. The objective of the present study was to test the hypothesis that this beneficial effect of proline was associated with alterations in inflammatory response at the placenta and fetus interface. Populations of immune cells present in peripheral blood mononuclear cells (PBMC) were determined by flow cytometry analysis. Read More

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http://dx.doi.org/10.1007/s00726-020-02837-0DOI Listing
April 2020
3.293 Impact Factor

γ-Glutamylcysteine synthetase and γ-glutamyl transferase as differential enzymatic sources of γ-glutamylpeptides in mice.

Amino Acids 2020 Apr 13;52(4):555-566. Epub 2020 Mar 13.

Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata, 990-9585, Japan.

Some γ-glutamylpeptides in blood plasma are putative biomarkers for pathological conditions of the liver. γ-Glutamyltransferase (GGT) and γ-glutamylcysteine synthetase (γ-GCS) are two such potential enzymes that are responsible for the production of γ-glutamylpeptides. GGT produces γ-glutamylpeptides by transferring the γ-glutamyl moiety from glutathione to an amino acid or a peptide. Read More

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http://dx.doi.org/10.1007/s00726-020-02835-2DOI Listing

Composition of amino acids and related nitrogenous nutrients in feedstuffs for animal diets.

Authors:
Peng Li Guoyao Wu

Amino Acids 2020 Apr 11;52(4):523-542. Epub 2020 Mar 11.

Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA.

We analyzed the composition of amino acids (AAs) in oligopeptides, proteins, and the free pool, as well as creatine, agmatine, polyamines, carnosine, anserine, and glutathione, in animal- and plant-derived feedstuffs. Ingredients of animal origins were black soldier fly larvae meal (BSFM), chicken by-product meal, chicken visceral digest, feather meal, Menhaden fishmeal, Peruvian anchovy fishmeal, Southeast Asian fishmeal, spray-dried peptone from enzymes-treated porcine mucosal tissues, poultry by-product meal (pet-food grade), spray-dried poultry plasma, and spray-dried egg product. Ingredients of plant origins were algae spirulina meal, soybean meal, and soy protein concentrate. Read More

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http://dx.doi.org/10.1007/s00726-020-02833-4DOI Listing

Negative effects of a low-quality protein diet on wound healing via modulation of the MMP2 activity in rats.

Amino Acids 2020 Apr 4;52(4):505-510. Epub 2020 Mar 4.

Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan.

Protein malnutrition is largely associated with a delay or failure of the healing process. However, the effect of dietary protein quality on wound healing is largely unknown. This study aimed to reveal the effect of dietary protein quality on wound healing and elucidate the regulatory mechanisms in a rat model of full-thickness cutaneous wounds. Read More

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http://dx.doi.org/10.1007/s00726-020-02831-6DOI Listing

Leucine and mTORc1 act independently to regulate 2-deoxyglucose uptake in L6 myotubes.

Amino Acids 2020 Mar 27;52(3):477-486. Epub 2020 Feb 27.

School of Biosciences, The University of Nottingham, Sutton Bonington campus, Loughborough, Leicestershire, LE12 5RD, UK.

Chronic mTORc1 hyperactivation via obesity-induced hyperleucinaemia has been implicated in the development of insulin resistance, yet the direct impact of leucine on insulin-stimulated glucose uptake in muscle cells remains unclear. To address this, differentiated L6 myotubes were subjected to various compounds designed to either inhibit mTORc1 activity (rapamycin), blunt leucine intracellular import (BCH), or activate mTORc1 signalling (3BDO), prior to the determination of the uptake of the glucose analogue, 2-deoxyglucose (2-DG), in response to 1 mM insulin. In separate experiments, L6 myotubes were subject to various media concentrations of leucine (0-0. Read More

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http://dx.doi.org/10.1007/s00726-020-02829-0DOI Listing

Taurine prevents cardiomyocyte apoptosis by inhibiting the calpain-1/cytochrome c pathway during RVH in broilers.

Amino Acids 2020 Mar 27;52(3):453-463. Epub 2020 Feb 27.

Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, People's Republic of China.

The calpain-1-activated apoptotic pathway plays a key role in right ventricular hypertrophy (RVH). Taurine has been shown to attenuate apoptosis by inhibiting calpain activity. This experiment aimed to determine whether taurine could prevent RVH by inhibiting the calpain-1/cytochrome c apoptotic pathway. Read More

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http://dx.doi.org/10.1007/s00726-020-02824-5DOI Listing

Involvement of penicillin-binding proteins in the metabolism of a bacterial peptidoglycan containing a non-canonical D-amino acid.

Amino Acids 2020 Mar 27;52(3):487-497. Epub 2020 Feb 27.

Graduate School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

Bacteria produce various D-amino acids, including non-canonical D-amino acids, to adapt to environmental changes and overcome a variety of threats. These D-amino acids are largely utilized as components of peptidoglycan, and they promote peptidoglycan remodeling and biofilm disassembly. The biosynthesis, maturation, and recycling of peptidoglycan are catalyzed by penicillin-binding proteins (PBPs). Read More

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http://dx.doi.org/10.1007/s00726-020-02830-7DOI Listing

RETRACTED ARTICLE: An insightful 20-year recollection since the birth of pseudo amino acid components.

Authors:
Kuo-Chen Chou

Amino Acids 2020 May 18;52(5):847. Epub 2020 Feb 18.

Gordon Life Science Institute, Boston, MA, 02478, USA.

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http://dx.doi.org/10.1007/s00726-020-02828-1DOI Listing

Important roles of dietary taurine, creatine, carnosine, anserine and 4-hydroxyproline in human nutrition and health.

Authors:
Guoyao Wu

Amino Acids 2020 Mar 18;52(3):329-360. Epub 2020 Feb 18.

Department of Animal Science and Faculty of Nutrition, Texas A&M University, College Station, TX, 77843-2471, USA.

Taurine (a sulfur-containing β-amino acid), creatine (a metabolite of arginine, glycine and methionine), carnosine (a dipeptide; β-alanyl-L-histidine), and 4-hydroxyproline (an imino acid; also often referred to as an amino acid) were discovered in cattle, and the discovery of anserine (a methylated product of carnosine; β-alanyl-1-methyl-L-histidine) also originated with cattle. These five nutrients are highly abundant in beef, and have important physiological roles in anti-oxidative and anti-inflammatory reactions, as well as neurological, muscular, retinal, immunological and cardiovascular function. Of particular note, taurine, carnosine, anserine, and creatine are absent from plants, and hydroxyproline is negligible in many plant-source foods. Read More

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http://dx.doi.org/10.1007/s00726-020-02823-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088015PMC

Biochemical and pathophysiological properties of polyamines.

Authors:
Enzo Agostinelli

Amino Acids 2020 Feb 18;52(2):111-117. Epub 2020 Feb 18.

Department of Biochemical Sciences, A. Rossi Fanelli', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.

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http://dx.doi.org/10.1007/s00726-020-02821-8DOI Listing
February 2020

Correction to: Synthesis, protonation constants and biological activity determination of amino acid-salicylaldehyde‑derived Schiff bases.

Amino Acids 2020 03;52(3):409

Department of Chemical and Geological Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, 09042, Cagliari, Italy.

Unfortunately in the online published article, the name of compound "L-salicylidenealanine" was published with incorrect spelling in the section "Synthesis of L‑salicylideneaniline (1a)". The section should correctly read as "Synthesis of L-salicylidenealanine". Read More

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http://dx.doi.org/10.1007/s00726-020-02826-3DOI Listing

Key amino acid residues involved in mammalian and insecticidal activities of Magi4 and Hv1b, cysteine-rich spider peptides from the δ-atracotoxin family.

Amino Acids 2020 Mar 17;52(3):465-475. Epub 2020 Feb 17.

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Apartado Postal 510-3, 62210, Cuernavaca Mor., México.

δ-Atracotoxins, also known as δ-hexatoxins, are spider neurotoxic peptides, lethal to both vertebrates and insects. Their mechanism of action involves the binding to of the S3/S4 loop of the domain IV of the voltage-gated sodium channels (Na). Because of the chemical difficulties of synthesizing folded synthetic δ-atracotoxins correctly, here we explore an expression system that is designed to produce biologically active recombinant δ-atracotoxins, and a number of variants, in order to establish certain amino acids implicated in the pharmacophore of this lethal neurotoxin. Read More

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http://dx.doi.org/10.1007/s00726-020-02825-4DOI Listing

Oral nutritional supplement prevents weight loss and reduces side effects in patients in advanced lung cancer chemotherapy.

Amino Acids 2020 Mar 7;52(3):445-451. Epub 2020 Feb 7.

Lab Experimental Oncology, Department of Biology, University of Tor Vergata, 00133, Rome, Italy.

Weight loss in patients with cancer is caused by cancer cachexia and chemotherapy-induced nausea and vomiting. Recent developments in antiemetic drugs have substantially improved nausea and vomiting, but this intervention did not reduce weight loss and other more severe side effects of chemotherapy, like anorexia, weakness, cough, dyspnea, hemoptysis, and pain. This study aimed to investigate the effects of nutrition intervention with a food supplement, during chemotherapy in patients with advanced nonsquamous non-small cell lung cancer (NSCLC). Read More

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http://dx.doi.org/10.1007/s00726-020-02822-7DOI Listing

Mobility of water and of protein atoms at the protein-water interface, monitored by anisotropic atomic displacement parameters, are largely uncorrelated.

Authors:
Oliviero Carugo

Amino Acids 2020 Mar 1;52(3):435-443. Epub 2020 Feb 1.

Department of Chemistry, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.

A non-redundant set of 231 protein crystal structures refined at a resolution better than (or equal to) 1 Å was extracted from the Protein Data Bank and the degree of conformational rigidity at the protein-water interface was examined by means of the Hirshfeld test and by comparing the orientations of the anisotropic Us for contacting protein and water atoms. Contacts between protein and water atoms are more rigid that contacts between water atoms and the degree of rigidity increases for shorter contacts and for more hydrogen-bonded atoms. Nevertheless, water and protein atoms are not rigidly held together. Read More

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http://dx.doi.org/10.1007/s00726-020-02820-9DOI Listing

Human cationic amino acid transporters are not affected by direct nitros(yl)ation.

Amino Acids 2020 Mar 1;52(3):499-503. Epub 2020 Feb 1.

Department of Pharmacology, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstraße 1, 55131, Mainz, Germany.

A direct inhibiting effect of NO on the function of CAT-1 and -2A has been postulated to occur via nitrosylation of cysteine residues in the transporters. Neither the NO donor SNAP nor a mixture of SIN-1 and Spermine NONOate, that generates the strong nitrosating agent NO, reduced CAT-mediated L-arginine transport. Direct nitros(yl)ation does either not occur in CATs or does not affect their transport function. Read More

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http://dx.doi.org/10.1007/s00726-020-02819-2DOI Listing

Physicochemical properties of L- and DL-valine: first-principles calculations.

Amino Acids 2020 Mar 1;52(3):425-433. Epub 2020 Feb 1.

Department of Bionanotechnology, Kemerovo State University, Krasnaya 6, Kemerovo, 650000, Russia.

At present, physicochemical properties of amino acid molecular crystals are of the utmost interest. The compounds where molecules have different chirality are the focus of particular interest. This paper, presents a study on the structural and electronic properties of crystalline L- and DL-valine within the framework of density functional theory including van der Waals interactions. Read More

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http://dx.doi.org/10.1007/s00726-020-02818-3DOI Listing

Inhibition of antigen-specific immune responses by co-application of an indoleamine 2,3-dioxygenase (IDO)-encoding vector requires antigen transgene expression focused on dendritic cells.

Amino Acids 2020 Mar 1;52(3):411-424. Epub 2020 Feb 1.

Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.

We have previously shown that particle-mediated epidermal delivery (PMED) of plasmids encoding β-galactosidase (βGal) under control of the fascin-1 promoter (pFascin-βGal) yielded selective production of the protein in skin dendritic cells (DCs), and suppressed Th2 responses in a mouse model of type I allergy by inducing Th1/Tc1 cells. However, intranasal challenge of mice immunized with pFascin-βGal induced airway hyperreactivity (AHR) and neutrophilic inflammation in the lung. The tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase (IDO) has been implicated in immune suppression and tolerance induction. Read More

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http://dx.doi.org/10.1007/s00726-020-02817-4DOI Listing

Synthesis, protonation constants and biological activity determination of amino acid-salicylaldehyde-derived Schiff bases.

Amino Acids 2020 Mar 13;52(3):397-407. Epub 2020 Jan 13.

Department of Chemical and Geological Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, 09042, Cagliari, Italy.

Schiff bases represent a class of molecules widely studied for their importance in organic and coordination chemistry. Despite the large amount of studies on the chemical and biological properties of the Schiff bases, the different experimental conditions prevent a useful comparison to search for a correlation structure-activity. Moreover, literature is lacking in comprehensive data on the spectroscopic characterization of these compounds. Read More

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http://dx.doi.org/10.1007/s00726-019-02816-0DOI Listing

Acetylcholinesterase inhibitory activity of a naturally occurring peptide isolated from Boana pulchella (Anura: Hylidae) and its analogs.

Amino Acids 2020 Mar 4;52(3):387-396. Epub 2020 Jan 4.

Laboratorio de Péptidos Bioactivos, Departamento de Química Orgánica, Facultad de Bioquímica Y Cs. Biológicas (FBCB), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, 3000, Santa Fe, Argentina.

Alzheimer's disease (AD), the most common form of dementia, is a growing problem worldwide, with 10 million incident cases registered every year. The complex etiology of AD has not been clarified yet and represents an active research topic. In this work, we studied the inhibitory properties of Hp-1935, a natural peptide extracted from the skin secretions of an Argentinian frog (Boana pulchella). Read More

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http://dx.doi.org/10.1007/s00726-019-02815-1DOI Listing

Hypermethioninemia induces memory deficits and morphological changes in hippocampus of young rats: implications on pathogenesis.

Amino Acids 2020 Mar 4;52(3):371-385. Epub 2020 Jan 4.

Programa de Pós-Graduação em Bioquímica e Bioprospecção-Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, 96010-900, Brazil.

The aim of this study was to investigate the effect of the chronic administration of methionine (Met) and/or its metabolite, methionine sulfoxide (MetO), on the behavior and neurochemical parameters of young rats. Rats were treated with saline (control), Met (0.2-0. Read More

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http://dx.doi.org/10.1007/s00726-019-02814-2DOI Listing
March 2020
3.293 Impact Factor

Design, synthesis and interaction of BRC4 analogous peptides with RAD51(241-260).

Amino Acids 2020 Mar 24;52(3):361-369. Epub 2019 Dec 24.

Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy, Northwest A&F University, Yangling, 712100, China.

Breast cancer susceptibility gene 2 (BRCA2) is an important tumor suppressor, which is participated in repair of damaged DNA by its highly conserved BRC repeat motifs regulating RAD51 protein homologous recombination and thereby preventing cell carcinogenesis. In this study, the BRCA2(1524-1548)-RAD51(241-260) complex structure was obtained based on PDB bank data 1N0W, which provided the basis for site-specific mutation of BRCA2(1524-1548). The BRC4 and BRC4 analogous peptides were synthesized, and the interaction between BRC peptide and RAD51(241-260) was studied by fluorescence spectroscopy, circular dichroism spectroscopy and microscale thermophoresis (MST). Read More

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http://dx.doi.org/10.1007/s00726-019-02813-3DOI Listing

Taurine inhibits neuron apoptosis in hippocampus of diabetic rats and high glucose exposed HT-22 cells via the NGF-Akt/Bad pathway.

Amino Acids 2020 Jan 24;52(1):87-102. Epub 2019 Dec 24.

Integrative Laboratory, Affiliated Zhong Shan Hospital of Dalian University, Dalian, 116001, China.

Type 2 Diabetes causes learning and memory deficits that might be mediated by hippocampus neuron apoptosis. Studies found that taurine might improve cognitive deficits under diabetic condition because of its ability to prevent hippocampus neuron apoptosis. However, the effect and mechanism is not clear. Read More

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http://dx.doi.org/10.1007/s00726-019-02810-6DOI Listing
January 2020
3.293 Impact Factor

Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.

Amino Acids 2020 Jan 18;52(1):73-85. Epub 2019 Dec 18.

Department of Neurology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246, Hamburg, Germany.

Statin-induced myopathy affects more than 10 million people worldwide. But discontinuation of statin treatment increases mortality and cardiovascular events. Recently, L-arginine:glycine amidinotransferase (AGAT) gene was associated with statin-induced myopathy in two populations, but the causal link is still unclear. Read More

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http://dx.doi.org/10.1007/s00726-019-02812-4DOI Listing
January 2020

Studies on the constituents of Helleborus purpurascens: use of derivatives from calix[6]arene, homooxacalix[3]arene and homoazacalix[3]arene as extractant agents for amino acids from the aqueous extract.

Amino Acids 2020 Jan 18;52(1):55-72. Epub 2019 Dec 18.

Institutul National de Cercetare Dezvoltare pentru Electrochimie si Materie Condensata, Str. Dr. A. Paunescu Podeanu, Nr. 144, 300569, Timisoara, Romania.

The task of this work was to investigate the extraction capacity of various calixarenes for free and esterified amino acids from aqueous acid phases. Furthermore, this method was applied to aqueous extracts of Helleborus purpurascens. Generally, it is known that calixarenes can be used as extractants for ammonium compounds due to π-cation and lone pair cation interactions. Read More

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http://dx.doi.org/10.1007/s00726-019-02809-zDOI Listing
January 2020

Asymmetric dimethylation and citrullination in the LEW.1AR1-iddm rat, an animal model of human type 1 diabetes, and effects of anti-TCR/anti-TNF-α antibody-based therapy.

Amino Acids 2020 Jan 12;52(1):103-110. Epub 2019 Dec 12.

Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany.

The LEW.1AR1-iddm rat is an animal model of human type 1 diabetes (T1D). We determined by GC-MS the extent of asymmetric dimethylation (prADMA) and citrullination (prCit) of L-arginine residues in organ proteins (pr) of normoglycaemic control (ngCo, n = 6), acutely diabetic (acT1D, n = 6), chronically diabetic (chT1D, n = 4), and cured (cuT1D, n = 4) rats after anti-TCR/anti-TNF-α therapy. Read More

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http://dx.doi.org/10.1007/s00726-019-02811-5DOI Listing
January 2020

Killing Streptococcus mutans in mature biofilm with a combination of antimicrobial and antibiofilm peptides.

Amino Acids 2020 Jan 3;52(1):1-14. Epub 2019 Dec 3.

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.

Biofilm poses a serious challenge for the treatment of bacterial infections, as it endows bacteria a pronounced resistance to traditional antibiotics. Antimicrobial peptides (AMPs) are considered potential substitutes for antibiotics. Combinational use of AMPs with other compounds to exert antibiofilm effects has been proved to be an effective means to reduce their toxicity and maximize their antimicrobial activity. Read More

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http://dx.doi.org/10.1007/s00726-019-02804-4DOI Listing
January 2020

Taurine attenuates Cr(VI)-induced cellular and DNA damage: an in vitro study using human erythrocytes and lymphocytes.

Amino Acids 2020 Jan 28;52(1):35-53. Epub 2019 Nov 28.

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.

Hexavalent chromium [(Cr(VI)] is widely used in several industries, but human exposure results in multiple organ toxicity. Enhanced generation of free radicals and reactive species is thought to play a key role in Cr(VI)-induced toxicity. We have examined the effect of taurine, a simple sulphur-containing amino acid and an antioxidant, on potassium dichromate [KCrO, a Cr(VI) compound]-induced cytotoxicity and genotoxicity in human blood cells. Read More

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http://dx.doi.org/10.1007/s00726-019-02807-1DOI Listing
January 2020