2,720 results match your criteria American journal of translational research[Journal]


pSTAT3 Y705 is a prognostic biomarker identified from time-series gene expression profiles of a chemically induced mouse model of hepatocellular carcinoma.

Am J Transl Res 2020 15;12(4):1443-1458. Epub 2020 Apr 15.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University Hangzhou, China.

Development of hepatocellular carcinoma (HCC) is a dynamic process that includes a spectrum ranging from precancerous exposure to carcinogenesis and metastasis stages. In this process, numerous dysregulated genes resulted in aberrant activation or inhibition of signaling pathways. Herein, time-series gene expression profiles of dimethylnitrosamine (DEN)-induced mice with HCC covering different stages are provided. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191170PMC

Integrin-associated CD151 is a suppressor of prostate cancer progression.

Am J Transl Res 2020 15;12(4):1428-1442. Epub 2020 Apr 15.

Department of Pharmacology and Nutritional Sciences, Department of Molecular and Cellular Biochemistry, and Markey Cancer Center, University of Kentucky Lexington 40536, KY, USA.

Owing to the complexity of interacting molecular networks on the cell surface, integrin-associated tetraspanin CD151 remains controversial regarding its clinical importance and functional impact in prostate cancer. The current study evaluated dynamics and clinical importance of CD151 expression and its function in prostate cancer by IHC analysis of two independent patient cohorts (n=80, 181), bioinformatic interrogation of the TCGA database, and evaluation of gene knockdown effect at the cellular level. Our data showed that aside from high mRNA expression, CD151 was primarily localized to intercellular junctions at the plasma membrane in normal prostate glands or benign tissues, regardless of nature of antibodies used. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191174PMC

Proteomic profiling and bioinformatics analysis identify key regulators during the process from fanconi anemia to acute myeloid leukemia.

Am J Transl Res 2020 15;12(4):1415-1427. Epub 2020 Apr 15.

Department of Hematology & Oncology, Children's Hospital of Soochow University Suzhou 215025, Jiangsu, China.

Fanconi anemia (FA) is a congenital aplastic anemia, characterized as congenital bone marrow failure, developmental malformation, and the malignant tendency, which may develop into acute myeloid leukemia (AML). However, few studies have been conducted on the progression from FA to AML. In this study, we used proteomic profiling, together with bioinformatics analyses, to explore the molecular mechanisms by which FA progresses to AML. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191167PMC

Role of RP11-83J16.1, a novel long non-coding RNA, in rheumatoid arthritis.

Am J Transl Res 2020 15;12(4):1397-1414. Epub 2020 Apr 15.

Department of Rheumatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine Shanghai, China.

This study aimed to explore the effects of long non-coding RNA (lncRNA) expression on rheumatoid arthritis (RA). LncRNA expression profiles were obtained from the synovial tissues of five RA patients and five age-/gender-matched controls by RNA-Seq. Six candidate lncRNAs were then chosen and their levels in synovial fluid further examined in 25 RA patients and 25 health controls using RT-qPCR. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191173PMC

Cerebrospinal fluid biomarkers for brain tumor detection: clinical roles and current progress.

Am J Transl Res 2020 15;12(4):1379-1396. Epub 2020 Apr 15.

Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University Nanchang 330006, Jiangxi, China.

Brain tumors include those that originate within the brain (primary tumors) as well as those that arise from other cancers (metastatic tumors). The fragile nature of the brain poses a major challenge to access focal malignancies, which certainly limits both diagnostics and therapeutic approaches. This limitation has been alleviated with the advent of liquid biopsy technologies. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191171PMC

Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.

Am J Transl Res 2020 15;12(4):1362-1378. Epub 2020 Apr 15.

Department of Thyroid Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University Guangzhou 510000, China.

Purpose: The present study aimed to identify differentially expressed circRNAs in thyroid cancer and verify their potential functions.

Methods: Next-generation sequencing was used to identify differentially expressed circRNAs between papillary thyroid carcinoma (PTC) tissues and paired pericarcinomatous tissues. Polymerase chain reaction and Sanger sequencing methods successfully identified hsa_circ_0007694. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191164PMC

Trends and prediction in daily incidence of novel coronavirus infection in China, Hubei Province and Wuhan City: an application of Farr's law.

Am J Transl Res 2020 15;12(4):1355-1361. Epub 2020 Apr 15.

Department of Pathology, Princeton Medical Center Plainsboro, NJ, USA.

Background: The recent outbreak of novel coronavirus (2019-nCoV) has infected tens of thousands of patients in China. Studies have forecasted future trends of the incidence of 2019-nCoV infection, but appeared unsuccessful. Farr's law is a classic epidemiology theory/practice for predicting epidemics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191162PMC
April 2020
3.226 Impact Factor

Clinical evaluation of a rapid colloidal gold immunochromatography assay for SARS-Cov-2 IgM/IgG.

Am J Transl Res 2020 15;12(4):1348-1354. Epub 2020 Apr 15.

Institute of Digestive Diseases, School of Medicine, Tongji University, Tongji Hospital Affiliated to Tongji University Shanghai 200065, China.

Background: Since December 2019, there had been an outbreak of COVID-19 in Wuhan, China. At present, diagnosis COVID-19 were based on real-time RT-PCR, which have to be performed in biosafe laboratory and is unsatisfactory for suspect case screening. Therefore, there is an urgent need for rapid diagnostic test for COVID-19. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191168PMC
April 2020
3.226 Impact Factor

LncRNA H19/Runx2 axis promotes VSMCs transition via MAPK pathway.

Am J Transl Res 2020 15;12(4):1338-1347. Epub 2020 Apr 15.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, The Key Laboratory of Remodeling-related Cardiovascular Disease, Ministry of Education Beijing, China.

Arterial calcification (AC) is mainly caused by osteoblast phenotypic transition of vascular smooth muscle cells (VSMCs). Long noncoding RNA H19 (lncRNA H19) has attracted increasingly attention because of their transcriptional regulation crucial potency. We reported that lncRNA H19 expression is up-regulated after VSMCs transition. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191176PMC
April 2020
3.226 Impact Factor

Corrective surgery alters plasma protein profiling in congenital heart diseases and clinical perspectives.

Am J Transl Res 2020 15;12(4):1319-1337. Epub 2020 Apr 15.

Department of Cardiovascular Surgery & Center for Basic Medical Research, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin, China.

The final goal for treatment of congenital heart diseases (CHD) is to resume not only the normal heart structure but also physiology. The present study evaluates surgical results at molecular basis on the proteomic pattern in the pre- and post-operative period in tetralogy of Fallot (TOF) and ventricular septal defect (VSD) in order to find whether structure repair is associated with clinically important molecular changes in CHD. Differential protein analysis by using two-dimensional gel electrophoresis and mass spectrometry followed by ELISA validation was performed in the plasma samples of patients with TOF (n=82) or VSD (n=82) preoperatively, 6-month postoperatively, and in normal controls (n=82). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191161PMC

Upregulation of cell division cycle 20 in cisplatin resistance-induced epithelial-mesenchymal transition in osteosarcoma cells.

Am J Transl Res 2020 15;12(4):1309-1318. Epub 2020 Apr 15.

Department of Orthopaedics, Cancer Hospital of China Medical University Shenyang, Liaoning Province, PR China.

Cell division cycle 20 homologue (Cdc20) is characterized as an oncoprotein that is involved in carcinogenesis. Accumulated evidence reveals that Cdc20 plays an oncogenic role by governing cell growth, apoptosis, motility, and metastasis. The role of Cdc20 in drug resistance is elusive. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191160PMC

ARNTL2 knockdown suppressed the invasion and migration of colon carcinoma: decreased SMOC2-EMT expression through inactivation of PI3K/AKT pathway.

Am J Transl Res 2020 15;12(4):1293-1308. Epub 2020 Apr 15.

Department of Gastroenterology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong, P. R. China.

ARNTL2 is a transcriptional activator implicated in the molecular clock feedback system and is overexpressed in some malignant tumors. This study aimed to detect the effects of ARNTL2 knockdown by siRNA on the proliferation and invasion of colon carcinoma and clarify the molecular mechanisms of ARNTL2 in the development of colon carcinoma (CC). The CC microarray dataset GSE50760 was downloaded from the Gene Expression Omnibus (GEO) database. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191172PMC

Reduced expression of enolase-1 correlates with high intracellular glucose levels and increased senescence in cisplatin-resistant ovarian cancer cells.

Am J Transl Res 2020 15;12(4):1275-1292. Epub 2020 Apr 15.

Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus San Juan 00935, Puerto Rico.

Despite good responses to first-line treatment with platinum-based combination chemotherapy, most ovarian cancer patients will relapse and eventually develop a platinum-resistant disease with a poor overall prognosis. The molecular events leading to the cisplatin resistance of ovarian cancer cells are not fully understood. Here, we performed a proteomic analysis to identify protein candidates deregulated in a cisplatin-resistant ovarian cancer cell line (A2780CP20) in comparison to their sensitive counterpart (A2780). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191177PMC
April 2020
3.226 Impact Factor

MicroRNA-520b targets interlukin-2 to regulate the growth, metastasis and drug sensitivity of liver cancer cells.

Am J Transl Res 2020 15;12(4):1267-1274. Epub 2020 Apr 15.

Department of Cancer Center, Beijing Ditan Hospital Capital Medical University No 8, Jingshundong Street, Chaoyang District, Beijing 100015, China.

Liver cancer is ranked as the 5 major type of cancer and is responsible for significant number of human deaths across the world. Recent investigations have shown microRNAs (miRs) to be involved in diverse cellular processes. Additionally, they have been shown to exhibit therapeutic implications in treatment of different diseases including cancer. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191169PMC

Combined therapy with ligustrazine and paeonol mitigates hepatic fibrosis through destroying mitochondrial integrity of stellate cell.

Am J Transl Res 2020 15;12(4):1255-1266. Epub 2020 Apr 15.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine Nanjing 210023, China.

This study investigates the inhibitory effect and potential mechanism of ligustrazine combined with paeonol on hepatic fibrosis, as to provide a new therapeutic strategy for clinical hepatic fibrosis. The degree of liver injury collagen deposition and inflammation was assessed by hematoxylin and eosin staining, Masson, Sirius red staining and biochemically serum analysis. ATP and ROS levels in each group were detected by chemical fluorescence method. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191175PMC

Promising diagnostic and prognostic value of six genes in human hepatocellular carcinoma.

Am J Transl Res 2020 15;12(4):1239-1254. Epub 2020 Apr 15.

Department of Pancreatic Surgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P.R. China.

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. Ample data have been reported to unravel the carcinogenesis over the past decades. Although pinpointing the cause of the HCC is challenging, this in and of itself may not be an insuperable problem. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191178PMC

Identification of biomarkers for the transition from low-grade glioma to secondary glioblastoma by an integrated bioinformatic analysis.

Am J Transl Res 2020 15;12(4):1222-1238. Epub 2020 Apr 15.

Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University Nanjing, Jiangsu, China.

Secondary glioblastoma (sGBM) is a type of glioblastoma multiforme that evolves from low-grade glioma (LGG). However, the mechanism of this transition still remains poorly understood. In this study, we used weighted gene co-expression network analysis (WGCNA) on the gene expression profiles of glioma samples from the Chinese Glioma Genome Atlas (CGGA) database to identify key genetic module related to distinguish histological characteristics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191179PMC
April 2020
3.226 Impact Factor

Effects of tristetraprolin on doxorubicin (adriamycin)-induced experimental kidney injury through inhibiting IL-13/STAT6 signal pathway.

Am J Transl Res 2020 15;12(4):1203-1221. Epub 2020 Apr 15.

Department of Nephrology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, P. R. China.

To study the effects of Tristetraprolin (TTP) on Doxorubicin (DOX)-induced experimental kidney injury (KI). DOX was used to induce kidney injury in Balb/c male mice () and in human kidney proximal tubular epithelial cell line (HK-2) and normal rat kidney epithelial cell line (NRK-52E) (in vitro). Body weight of experimental mice were recorded daily. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191163PMC

Combined signature of nine immune-related genes: a novel risk score for predicting prognosis in hepatocellular carcinoma.

Am J Transl Res 2020 15;12(4):1184-1202. Epub 2020 Apr 15.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanchang University Nanchang, Jiangxi, People's Republic of China.

Hepatocellular carcinoma (HCC) is one of the most common internal malignancies worldwide and is associated with a poor prognosis. There is an urgent need to identify diagnostic and prognostic biomarkers of HCC pathogenesis and progression. Accordingly, in this study, we analyzed differentially expressed immune-related genes (IRGs) from 329 patients with HCC from The Cancer Genome Atlas datasets. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191166PMC

Update on the role of endoplasmic reticulum stress in asthma.

Am J Transl Res 2020 15;12(4):1168-1183. Epub 2020 Apr 15.

Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Key Cite of National Clinical Research Center for Respiratory Disease, Wuhan Clinical Medical Research Center for Chronic Airway Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology 1095 Jiefang Ave, Wuhan 430030, China.

Asthma has long attracted extensive attention because of its recurring symptoms of reversible airflow obstruction, airway hyperresponsiveness (AHR) and airway inflammation. Although accumulating evidence has enabled gradual increases in understanding of the pathogenesis of asthma, many questions regarding the mechanisms underlying asthma onset and progression remain unanswered. Recent advances delineating the potential functions of endoplasmic reticulum (ER) stress in meeting the need for an airway hypersensitivity response have revealed critical roles of unfolded protein response (UPR) pathways in asthma. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191165PMC
April 2020
3.226 Impact Factor

Erratum: Cenicriviroc ameliorates the severity of graft-versus host disease through inhibition of CCR5 in a rat model of liver transplantation.

Am J Transl Res 2020 15;12(3):1166. Epub 2020 Mar 15.

Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008, Jiangsu Province, China.

[This corrects the article on p. 3438 in vol. 11, PMID: 31312356. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137054PMC

Oncology and functional prognosis are both vital in the surgical treatment of RGCTs around the knee joint.

Am J Transl Res 2020 15;12(3):1155-1165. Epub 2020 Mar 15.

Department of Orthopaedics, Xiangya Hospital, Central South University 87th Xiangya Road, Changsha, Hunan, China.

Aims: To explore and provide reasonable surgical options for recurrent giant cell tumors of bone (RGCTs) around the knee joint and compare the pros and cons of extended curettage (EC) and segmental resection (SR).

Materials And Methods: A retrospective analysis was performed of 22 patients (11 male, 11 female; mean age, 34.1 years) with RGCT around the knee joint treated in our hospital between June 2007 and June 2017. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137064PMC
March 2020
3.226 Impact Factor

MicroRNA-29 family inhibits rhabdomyosarcoma formation and progression by regulating GEFT function.

Am J Transl Res 2020 15;12(3):1136-1154. Epub 2020 Mar 15.

Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, China.

The microRNA-29 family, which contains mir-29a, mir-29b, and mir-29c, can promote or resist the development of several types of tumors. However, its role in rhabdomyosarcoma (RMS) has not been determined. In this work, we detected the expression of mir-29a/b/c in RMS. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137044PMC
March 2020
3.226 Impact Factor

C16:0 ceramide effect on melanoma malignant behavior and glycolysis depends on its intracellular or exogenous location.

Am J Transl Res 2020 15;12(3):1123-1135. Epub 2020 Mar 15.

Department of Plastic and Reconstructive Surgery, Third Xiangya Hospital of Central South University Changsha, P. R. China.

To investigate the role of C16:0 ceramide in melanoma metastatic behavior and glycolysis, five common long-chain ceramides (C16:0, C18:0, C20:0, C22:0, C24:0) were tested in melanocyte and melanoma cell lines by LC-MS. We then treated non-metastatic and metastatic melanoma cells with PDMP and exogenous C16:0 to explore their effects on proliferation, migration, and glycolysis. The long-chain ceramide was also analyzed by LC-MS after treatment. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137069PMC
March 2020
3.226 Impact Factor

Axin1 inhibits proliferation, invasion, migration and EMT of hepatocellular carcinoma by targeting miR-650.

Am J Transl Res 2020 15;12(3):1114-1122. Epub 2020 Mar 15.

Department of Hepatobiliary Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou 215002, Jiangsu, P. R. China.

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high mortality rate and low survival rate. This study was designed to explore a novel molecular with high sensitivity and specificity, which can be applied in early diagnosis and therapeutic evaluation of HCC. The current study aims to investigate the effect and important role of Axin1 on cell proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137066PMC

Selective EP2 and Cox-2 inhibition suppresses cell migration by reversing epithelial-to-mesenchymal transition and Cox-2 overexpression and E-cadherin downregulation are implicated in neck metastasis of hypopharyngeal cancer.

Am J Transl Res 2020 15;12(3):1096-1113. Epub 2020 Mar 15.

Department of Otorhinolaryngology-Head and Neck Surgery, Keio University School of Medicine Tokyo, Japan.

Cyclooxygenase-2 (Cox-2) has been shown to promote cancer initiation and progression through pleiotropic functions including induction of epithelial-to-mesenchymal transition (EMT) via its predominant product prostaglandin E that binds to the cognate receptor EP2. Hence, pharmacological inhibition at the level of EP2 is assumed to be a more selective alternative with less risk to Cox-2 inhibition. However, little is known regarding the anti-cancer effect of an EP2 antagonist on the malignant properties of cancers including hypopharyngeal squamous cell carcinoma (HPSCC). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137058PMC

Exosome-mediated miRNA delivery promotes liver cancer EMT and metastasis.

Am J Transl Res 2020 15;12(3):1080-1095. Epub 2020 Mar 15.

Department of Interventional Radiology, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, China.

The deregulation of exosomal microRNAs (miRNAs) plays an important role in the progression of hepatocarcinogenesis. In this study, we highlight exosomes as mediators involved in modulating miRNA profiles in liver cancer cells after induction of the epithelial-mesenchymal transition (EMT) and metastasis. Initially, we induced EMT in a hepatocellular carcinoma cell (HCC) line (Hep3B) by stimulation with transforming growth factor-β (TGF-β) and confirmed by western blot detection of EMT markers such as vimentin and E-cadherin. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137059PMC

Three-dimensional-printed titanium alloy porous scaffold combined with trans-cinnamaldehyde for repairing osteonecrosis of the femoral head in a dog model.

Am J Transl Res 2020 15;12(3):1070-1079. Epub 2020 Mar 15.

Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, Jiangsu, China.

Osteonecrosis of the femoral head (ONFH) is a common disorder that may be idiopathic, caused by trauma, or associated with alcohol or glucocorticoid use. The goals of early treatment include delaying or avoiding hip replacement, but there are no effective treatments for early-stage disease. The aim of the present study was to evaluate the effects of treatment with 3D-printed porous titanium alloy scaffold combined with daily intraperitoneal trans-cinnamaldehyde (TCA) in a dog model of ONFH. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137042PMC
March 2020
3.226 Impact Factor

Targeted and sustained Sox9 expression in mouse hypertrophic chondrocytes causes severe and spontaneous osteoarthritis by perturbing cartilage homeostasis.

Am J Transl Res 2020 15;12(3):1056-1069. Epub 2020 Mar 15.

Department of Orthopaedics, Case Western Reserve University Cleveland, OH, USA.

Sox9 is the master transcription factor essential for cartilage development and homeostasis. To investigate the specific role of Sox9 during chondrocyte hypertrophy, we generated a novel transgenic mouse model, in which is specifically expressed in hypertrophic chondrocytes driven by a well-characterized 10-kb promoter. These mice were viable and fertile, and appeared normal at birth. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137053PMC

Circulating tumor DNA in colorectal cancer: opportunities and challenges.

Am J Transl Res 2020 15;12(3):1044-1055. Epub 2020 Mar 15.

Medical Oncology Department of Gastrointestinal Cancer, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute Shenyang, China.

Tumor biopsy is the standard method for cancer diagnosis and provides an important sample for pathological assessment. With the development of precision medicine, liquid biopsies are now an important tool to detect molecular changes and tumor heterogeneity. In recent years, research related to circulating tumor DNA (ctDNA) has intensified due to its non-invasive, convenient, comprehensive, and safety characteristics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137038PMC

Dinaciclib prolongs survival in the ; ; (KPC) transgenic murine models of pancreatic ductal adenocarcinoma.

Am J Transl Res 2020 15;12(3):1031-1043. Epub 2020 Mar 15.

Department of Radiology, Feinberg School of Medicine, Northwestern University Chicago, IL, USA.

Dinaciclib is a small molecule cyclin-dependent kinase inhibitor with the potential to treat multiple cancers. To better understand its cytotoxic action in pancreatic ductal adenocarcinoma (PDAC), we evaluated dinaciclib therapeutic effects in the transgenic mouse model ( ; ; mice; KPC mice). Tumor growth and microenvironment were dynamically monitored by magnetic resonance imaging (MRI). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137051PMC

The extracellular signal-regulated kinase 1/2 modulates the intracellular localization of DNA methyltransferase 3A to regulate erythrocytic differentiation.

Am J Transl Res 2020 15;12(3):1016-1030. Epub 2020 Mar 15.

Department and Institute of Physiology, National Yang-Ming University Taipei 11221, Taiwan.

DNA methylation, catalyzed by DNA methyltransferases (DNMTs), is a heritable epigenetic mark, participating in numerous physiological processes. DNMT3A is of particular relevance to hematopoietic differentiation, because mutations are strongly related to hematopoietic malignancies. Additionally, DNMT3A deficiency has been reported to increase the hematopoietic stem cell pool by limiting their differentiation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137067PMC

Long-term effect of extracorporeal shock wave therapy on attenuating radiation-induced chronic cystitis in rat.

Am J Transl Res 2020 15;12(3):999-1015. Epub 2020 Mar 15.

Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Kaohsiung, Taiwan.

Background: This study tested the long-term effect of extracorporeal shock wave (ECSW) therapy on ameliorating radiotherapy-induced chronic cystitis (CC) in rat.

Methods And Results: Adult-female SD rats (n = 24) were equally categorized into group 1 (normal control), group 2 (CC induced by radiotherapy with 450 cGy twice with a four-hour interval to the urinary bladder), group 3 [CC with ECSW treatment (0.1 mJ/mm/120 impulses once every 3 days after radiotherapy)]. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137039PMC
March 2020
3.226 Impact Factor

Huangqi-Danshen decoction alleviates diabetic nephropathy in mice by inhibiting PINK1/Parkin-mediated mitophagy.

Am J Transl Res 2020 15;12(3):989-998. Epub 2020 Mar 15.

Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine Shenzhen 518033, Guangdong, China.

Huangqi-Danshen decoction (HDD) is composed of Astragali Radix (Huang-qi) and Salviae Miltiorrhizae Radix et Rhizoma (Dan-shen), both of which are the most commonly used herbs for the clinical treatment of diabetic nephropathy (DN) in traditional Chinese medicine and show good efficacy. However, the underlying mechanism of this effect is unclear. The aim of this study was to evaluate the effect and potential mechanism of HDD in the treatment of DN in a type 2 diabetic animal model, mice. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137035PMC

Immunotherapy in anaplastic thyroid cancer.

Am J Transl Res 2020 15;12(3):974-988. Epub 2020 Mar 15.

Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, China.

Anaplastic thyroid cancer (ATC) is one of the worst human malignancies, with an associated median survival of only 5 months. It is resistant to conventional thyroid cancer therapies, including radioiodine and thyroid-stimulating hormone suppression. Cancer immunotherapy has emerged over the past few decades as a transformative approach to treating a wide variety of cancers. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137046PMC
March 2020
3.226 Impact Factor

Changed hemodynamics in acute vasoreactivity testing: prognostic predictors in chronic thromboembolic pulmonary hypertension.

Am J Transl Res 2020 15;12(3):959-973. Epub 2020 Mar 15.

Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University, School of Medicine Shanghai, China.

Chronic thromboembolic pulmonary hypertension (CTEPH) is similar to pulmonary arterial hypertension (PAH) in its pathogenesis. Changed hemodynamic parameters in acute vasoreactivity testing (AVT) have proved to be prognostic predictors of PAH. We wanted to determine whether these changed indices also impacted the prognosis of CTEPH. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137036PMC
March 2020
3.226 Impact Factor

Long noncoding RNA HAS2-AS1 accelerates non-small cell lung cancer chemotherapy resistance by targeting LSD1/EphB3 pathway.

Am J Transl Res 2020 15;12(3):950-958. Epub 2020 Mar 15.

Department of Oncology, The Second Hospital of Dalian Medical University Dalian, China.

The essential roles of long noncoding RNA (lncRNA) have been identified by emerging literature in the non-small cell lung cancer (NSCLC). However, the role of lncRNA hyaluronan synthase 2 antisense 1 (HAS2-AS1) in the NSCLC tumorigenesis is not clear. Here, we investigate the role and mechanism of HAS2-AS1 in the NSCLC tumorigenesis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137060PMC

Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1.

Am J Transl Res 2020 15;12(3):940-949. Epub 2020 Mar 15.

Department of Respiratory Medicine, Capital Medical University Beijing 100054, P. R. China.

Idiopathic pulmonary fibrosis (IPF) is a devastating disease, which is characterized by the progressive deterioration in lung function. In the pathogenesis of IPF, insulin-like growth factor-1 (IGF-1) has been found to be heavily involved. Metformin, a commonly used oral antidiabetic agent, is known to inhibit IGF-1 by the reversal of hyperinsulinemia. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137034PMC

Exploring cisplatin resistance in ovarian cancer through integrated bioinformatics approach and overcoming chemoresistance with sanguinarine.

Am J Transl Res 2020 15;12(3):923-939. Epub 2020 Mar 15.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Kunming Medical University Kunming, Yunnan Province, P. R. China.

Ovarian cancer is refractory in response towards platinum-based chemotherapy, and resistance frequently develops. We attempted to identify the driving pathways in cisplatin-resistant ovarian cancer and develop targeted therapies to overcome this resistance. Using an integrated bioinformatics approach, a GSE15372 database from NCBI's Gene Expression Omnibus database was obtained for identifying differentially expressed genes (DEGs), in which 535 DEGs were found (407 up-regulated and 128 down-regulated) in association with ovarian cancer cisplatin-resistance. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137043PMC

Role of mC-related regulatory genes in the diagnosis and prognosis of hepatocellular carcinoma.

Am J Transl Res 2020 15;12(3):912-922. Epub 2020 Mar 15.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, P. R. China.

Hepatocellular carcinoma (HCC) is one of the most common malignancies globally, and is frequently associated with a poor prognosis. 5-methylcytosine (mC) is a common epigenetic modification with many critical roles in eukaryotes. However, the expression and functional roles of mC regulators are largely unknown. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137052PMC
March 2020
3.226 Impact Factor

Assessment of inflammatory markers and mitochondrial factors in a rat model of sepsis-induced myocardial dysfunction.

Am J Transl Res 2020 15;12(3):901-911. Epub 2020 Mar 15.

Department of Emergency, Shenzhen Second People's Hospital, Shenzhen University First Affiliated Hospital No. 3002, Sungang West Road, Shenzhen 518035, Guangdong, P. R. China.

The present study aimed to investigate the expression of inflammatory markers and mitochondrial function-related genes, as well as their temporal relationship with cardiac myocyte injury in a rat model of sepsis. The sepsis model was constructed using cecal ligation and puncture (CLP). Two hours after CLP, the levels of inflammatory cytokines (interleukin [IL]-1β, IL-6, and TNFα) and myocardial function markers (serum brain natriuretic peptide [BNP], cardiac troponin-I [cTNI], and procalcitonin [PCT]) were increased significantly, falling from around 9 hours postoperatively. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137057PMC

CD19CD24CD38 regulatory B cells: a potential immune predictive marker of severity and therapeutic responsiveness of hepatitis C.

Am J Transl Res 2020 15;12(3):889-900. Epub 2020 Mar 15.

Department of Internal Medicine, Ohio State University College of Medicine and Wexner Medical Center Columbus, OH 43210, United States.

Objectives: Hepatitis C virus (HCV) infection is associated with abnormal immune responses. Since regulatory T (Tregs) and B (Bregs) cells modulate the progression of infectious diseases, this study aimed at examining how these cells are involved with the development of HCV infection.

Methods: The frequencies of circulating Bregs and Tregs were characterized using flow cytometry. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137049PMC

MiR-212-3p suppresses high-grade serous ovarian cancer progression by directly targeting MAP3K3.

Am J Transl Res 2020 15;12(3):875-888. Epub 2020 Mar 15.

Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi, China.

MicroRNAs (miRNAs) are small regulatory non-coding RNAs that have been reported to play an important role in the tumorigenesis of many cancers. In addition, miRNAs might serve as new promising biomarkers for diagnosis and prognosis and as effective therapeutic targets for patients with such malignancies. Accordingly, the dysregulation of miR-212-3p has been reported in a variety of human cancers. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137041PMC
March 2020
3.226 Impact Factor

MicroRNA-143 suppresses the proliferation and metastasis of human gastric cancer cells via modulation of STAT3 expression.

Am J Transl Res 2020 15;12(3):867-874. Epub 2020 Mar 15.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University Changchun 130033, Jilin, China.

Accumulating evidences suggest that miRNAs may prove essential therapeutic targets for the treatment of cancer. Herein, the role and therapeutic implications of miRNA-143 was investigated in gastric cancer. The results revealed miRNA-143 to be aberrantly downregulated in gastric cancer cell lines. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137056PMC

LncARSR promotes non-small-cell lung cancer progression via regulating PTEN/Akt.

Am J Transl Res 2020 15;12(3):857-866. Epub 2020 Mar 15.

Department of Occupational Disease, Daqing People's Hospital Daqing 163316, Heilongjiang, China.

LncRNAs have been suggested to be key modulators in many biological and pathological processes. LncARSR, a recently identified lncRNA, plays crucial roles in the progression of several cancers. However, the role of lncARSR in NSCLC is uninvestigated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137040PMC

Concurrent inhibition of ErbB family and MEK/ERK kinases to suppress non-small cell lung cancer proliferation.

Am J Transl Res 2020 15;12(3):847-856. Epub 2020 Mar 15.

Guangxi Medical University Nanning, Guangxi, China.

Lung cancer ranks as the most common cancer and leading cause of cancer-related deaths worldwide. Of all lung cancer types, non-small cell lung cancer (NSCLC) accounts for 85 percent of all cases. The high mortality of NSCLC occurs mainly because of poor prognosis in patients with recurrent and metastatic cancer. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137063PMC

L1CAM is involved in lymph node metastasis via ERK1/2 signaling in colorectal cancer.

Am J Transl Res 2020 15;12(3):837-846. Epub 2020 Mar 15.

College of Pharmaceutical Sciences, Zhejiang Chinese Medical University Hangzhou 310014, Zhejiang, China.

L1-cell adhesion molecule (L1CAM, L1) belongs to the immunoglobulin superfamily and was originally found to play a role in nerve cells. Recently, the expression and prognostic value of L1 has been established in several cancers, including colorectal cancer (CRC). However, its association with lymph node metastasis in CRC and the mechanisms underlying its effects remain unclear. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137048PMC

CXCR3 confers sorafenib resistance of HCC cells through regulating metabolic alteration and AMPK pathway.

Am J Transl Res 2020 15;12(3):825-836. Epub 2020 Mar 15.

Department of Gastroenterology, Henan Province People's Hospital, Zhengzhou University People's Hospital Zhengzhou, Henan, China.

Currently, the acquired resistance of the hepatocellular carcinoma (HCC) first-line therapeutic agent-sorafenib (SOR) remains a major challenge for HCC management. Recent evidence suggested the association between CXCL/CXCRs chemokines and chemotherapy resistant in cancer cells. Hence, exploring the internal mechanism of CXCRs involved in SOR resistance will help to improve the efficacy of HCC. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137055PMC

Identification of differentially expressed microRNAs and their target genes in the hippocampal tissues of Fmr1 knockout mice.

Am J Transl Res 2020 15;12(3):813-824. Epub 2020 Mar 15.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou, China.

Fragile X syndrome (FXS) is one of the most common forms of inherited mental retardation; it is usually associated with the transcriptional silencing of the Fmr1 gene and loss of its encoded protein, the fragile X mental retardation protein (FMRP). FMRP is an RNA-binding protein and participates in regulating the development of dendritic spines and synaptic plasticity. To uncover the possible role of microRNAs (miRNAs) in FXS and their relationship with FMRP, we used microarray analysis to investigate the miRNA expression profiles in the hippocampal tissues of Fmr1 knockout (Fmr1-KO) mice and wild type (WT) mice. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137065PMC