74 results match your criteria American journal of stem cells[Journal]


Impact of donor characteristics on the quality of bone marrow as a source of mesenchymal stromal cells.

Am J Stem Cells 2018 1;7(5):114-120. Epub 2018 Dec 1.

Immunobiology and Cell Biology Group, Department of Microbiology, Science Faculty, Pontificia Universidad Javeriana Bogotá D.C., Colombia, South America.

In recent years, the therapeutic use of mesenchymal stromal cells (MSC) has generated a valuable number of scientific studies that delve into their biological characteristics and their potential in regenerative medicine; however, the impact of the clinical characteristics of tissue donors, from which these cells are isolated, on their potential in applied clinical research is not yet clear. The objective of this study was to evaluate the impact of the clinical characteristics of bone marrow donors on the quality of this tissue as a source of MSC for therapeutic use. Human MSC were isolated, characterized and cultured (according to ISCT criteria) from bone marrow samples from volunteer donors (n = 70) attending the Department of Orthopedics and Traumatology of the Hospital Universitario San Ignacio (Bogota, Colombia) for surgery of prosthetic hip replacement that agreed to participate voluntarily in the study. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334204PMC
December 2018

Current approaches in regenerative medicine for the treatment of diabetes: introducing CRISPR/CAS9 technology and the case for non-embryonic stem cell therapy.

Am J Stem Cells 2018 1;7(5):104-113. Epub 2018 Dec 1.

Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center 3900 Reservoir Road NW, Washington DC 20007, USA.

Type 1 diabetes mellitus (T1DM) is an autoimmune disorder in which the body destroys its pancreatic β cells. Since these cells are responsible for insulin production, dysfunction or destruction of these cells necessitates blood glucose control through exogenous insulin shots. Curative treatment involves pancreas transplantation, but due to the incidence of transplant rejection and complications associated with immunosuppression, alternatives are being explored. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334205PMC
December 2018
3 Reads

Therapeutic applications of adipose-derived stem cells in cardiovascular disease.

Am J Stem Cells 2018 1;7(4):94-103. Epub 2018 Oct 1.

Department of Biology, American University Washington, DC 20016, USA.

Cardiovascular disease (CVD) is the number one cause of death globally, and new therapeutic techniques outside of traditional pharmaceutical and surgical interventions are currently being developed. At the forefront is stem cell-centered therapy, with adipose derived stem cells (ADSCs), an adult stem population, providing significant clinical promise. When introduced into damaged heart tissue, ADSCs promote cardiac regeneration by a variety of mechanisms including differentiation into new cardiomyocytes and secretion of paracrine factors acting on endogenous cardiac cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261868PMC
October 2018
16 Reads

A review of clinical trials: mesenchymal stem cell transplant therapy in type 1 and type 2 diabetes mellitus.

Am J Stem Cells 2018 1;7(4):82-93. Epub 2018 Oct 1.

Division of Endocrinology, Georgetown University Washington, DC 20007, USA.

Type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) are widely prevalent metabolic diseases with differing pathologies. T1DM manifests due to autoimmune destruction of the pancreatic beta cells, resulting in a diminished secretion of insulin. T2DM originates from a state of insulin resistance, resulting in hyperglycemia and reduction in beta cell mass. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261870PMC
October 2018
11 Reads

Close yet so far away: a look into the management strategies of genetic imprinting disorders.

Am J Stem Cells 2018 1;7(4):72-81. Epub 2018 Oct 1.

Department of Radiation Medicine/Oncology, MedStar Georgetown University Hospital Washington, DC, USA.

Genetic imprinting is the process of epigenetic labelling or silencing of particular genes, based on the maternal or paternal origin of the gene, in a heritable pattern. The incidence of imprinting disorders has become a growing concern due to the potential association between these congenital syndromes and assisted reproductive technologies (ARTs). This review presents a general summary of the imprinting process as well as the current knowledge surrounding the genetic and epigenetic underpinnings of the most prevalent imprinting disorders: Beckwith-Wiedemann syndrome (BWS), Silver-Russell syndrome (SRS), Prader-Willi syndrome (PWS), and Angelman syndrome (AS). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261869PMC
October 2018
8 Reads

Stem cell therapy in chronic obstructive pulmonary disease. How far is it to the clinic?

Am J Stem Cells 2018 1;7(3):56-71. Epub 2018 Aug 1.

Department of Pulmonary and Critical Care Medicine and Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center, University of Ulsan College of Medicine Seoul, South Korea.

Chronic obstructive pulmonary disease (COPD) is a respiratory disease that has a major impact worldwide. The currently-available drugs mainly focus on relieving the symptoms of COPD patients. However, in the latter stages of the disease, the airways become largely obstructed and lung parenchyma becomes destructed due to underlying inflammation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146161PMC
August 2018
13 Reads

Analysis of the regulation pathways via microarray and miRNA studies: human embryonic stem cells to treat diabetes mellitus type-II.

Am J Stem Cells 2018 1;7(2):48-55. Epub 2018 Jun 1.

Internal Medicine, Indraprastha Apollo Hospital New Delhi, India.

Diabetes mellitus occurs either due to an autoimmune destruction of β cells (Type 1) or resistance to insulin effects (Type 2). Diverse conventional medications are used for treatment of diabetes, which is associated with long term complications such as kidney failure, blindness, and stroke. We recently showed the potential of human embryonic stem cells (hESCs) in 95 patients with type 2 diabetes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013722PMC
June 2018
6 Reads

Comparing stemness gene expression between stem cell subpopulations from peripheral blood and adipose tissue.

Am J Stem Cells 2018 1;7(2):38-47. Epub 2018 Jun 1.

Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud Morones Prieto 3000 Pte. Monterrey NL. CP64710, México.

Cell therapy presents a promising alternative for the treatment of degenerative diseases. The main sources of adult stem cells are bone marrow, adipose tissue and peripheral blood. Within those tissues, there are cell subpopulations that share pluripotential characteristics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013721PMC
June 2018
5 Reads

Discovery of a stem-like multipotent cell fate.

Am J Stem Cells 2018 1;7(2):25-37. Epub 2018 Jun 1.

Department of Biology, American University 4400 Massachusetts Ave NW, Washington DC 20016, USA.

Adipose derived stem cells (ASCs) can be obtained from lipoaspirates and induced to differentiate into bone, cartilage, and fat. Using this powerful model system we show that after adipose differentiation a population of cells retain stem-like qualities including multipotency. They are lipid (-), retain the ability to propagate, express two known stem cell markers, and maintain the capacity for trilineage differentiation into chondrocytes, adipocytes, and osteoblasts. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013723PMC
June 2018
1 Read

Human corneal endothelial cell transplantation using nanocomposite gel sheet in bullous keratopathy.

Am J Stem Cells 2018 1;7(1):18-24. Epub 2018 Feb 1.

The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM)Chennai, Tamil Nadu, India.

Transplantation of expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine's cornea has been earlier reported. Herein we report the transplantation of HCEP cells derived from a cadaver donor cornea to three patients using the NC gel sheet. In three patients with bullous keratopathy, one after cataract surgery, one after trauma and another in the corneal graft, earlier performed for congenital corneal dystrophy, not amenable to medical management HCEP cells isolated from a human cadaver donor cornea expanded using a thermoreversible gelation polymer (TGP) for 26 days were divided into three equal portions and 1. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840311PMC
February 2018

Short-term hypoxia improves early cardiac progenitor cell function .

Am J Stem Cells 2018 1;7(1):1-17. Epub 2018 Feb 1.

Department of Pathology and Human AnatomyLoma Linda, CA.

The use of cardiovascular progenitor cells (CPCs) to repair damaged myocardium has been the focus of intense research. Previous reports have shown that pretreatments, including hypoxia, improve cell function. However, the age-dependent effects of short-term hypoxia on CPCs, and the role of signaling in these effects, are unknown. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840310PMC
February 2018
6 Reads

Role of cord blood and bone marrow mesenchymal stem cells in recent deep burn: a case-control prospective study.

Am J Stem Cells 2017 1;6(3):23-35. Epub 2017 Oct 1.

Department of Clinical Pathoogy, Cairo UniversityCairo, Egypt.

Rationale: Burn injuries represent one of the major worldwide public health problems causing more severe physiological stress than other traumas. Effective treatment of burn injuries is mandatory to prevent the numerous life-threatening complications and possible disabilities. Stem cells, a population of multipotent cells retaining the properties of self-renewal and differentiation, are the main player in tissue regeneration after major trauma. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675835PMC
October 2017
4 Reads

Zika Virus (ZIKV): a review of proposed mechanisms of transmission and associated congenital abnormalities.

Am J Stem Cells 2017 25;6(2):13-22. Epub 2017 Jul 25.

Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical CenterWashington 20007, DC, USA.

Zika virus (ZIKV) has been of major international public health concern following large outbreaks in the Americas occurring in 2015-2016. Most notably, ZIKV has been seen to pose dangers in pregnancy due to its association with congenital abnormalities such as microcephaly. Numerous experimental approaches have been taken to address how the virus can cross the placenta, alter normal fetal development, and disrupt specific cellular functions. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545216PMC
July 2017
7 Reads

A review of the emerging potential therapy for neurological disorders: human embryonic stem cell therapy.

Am J Stem Cells 2017 15;6(1):1-12. Epub 2017 Apr 15.

Department of Administration, Nutech Mediworld, H-8, Green Park ExtensionNew Delhi 110016, India.

The first human embryonic stem cell (hESC) line was developed in the late nineties. hESCs are capable of proliferating indefinitely and differentiate into all the three embryonic germ layers. Further, the differentiation of hESC lines into neural precursor cells and neurons, astrocytes and oligodendrocytes showed their potential in treating several incurable neurological disorders such as spinal cord injury (SCI), cerebral palsy (CP), Parkinson's disease (PD). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435646PMC
April 2017
33 Reads

Use of nano-sized clay crystallites to restore adhesion among tumor and aging stem cells - a molecular simulations approach.

Am J Stem Cells 2016 30;5(4):107-115. Epub 2016 Nov 30.

Department of Civil and Environmental Engineering, King Fahd University of Petroleum & Minerals (KFUPM) Dhahran, Saudi Arabia.

Adhesion of cells to the ECM is key to the regulation of cellular morphology, migration, proliferation, survival, and differentiation. The decrease in or loss of the cell's ability of mutual adhesiveness has been considered as one of the specific abnormalities in the surface properties of malignant cells. A change in the association of plasma membrane with cytoskeletal structures also seems to have a close relation with these abnormalities. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218867PMC
November 2016

Stepping back to move forward: a current review of iPSCs in the fight against Alzheimer's disease.

Am J Stem Cells 2016 20;5(3):99-106. Epub 2016 Oct 20.

Georgetown University Medical Center, Georgetown University School of Medicine Washington DC, USA.

The successful generation of the first iPSCs about ten years ago has provided deeper insight into previously unknown disease mechanisms and therapeutic opportunities for many diseases. In particular, iPSCs are becoming an important tool in advancing modeling and therapeutic intervention for Alzheimer's disease. In this manuscript, we assess the research climate surrounding the application of iPSCs to familial and sporadic Alzheimer's disease, including the generation and isolation of individualized neural stem cells, the introduction of neural stem cell transplants using iPSCs, and an estimation of the potential use of iPSCs as research models for Alzheimer's treatments and therapies. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107654PMC
October 2016

Current stem cell based therapies in diabetes.

Am J Stem Cells 2016 20;5(3):87-98. Epub 2016 Oct 20.

Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center Washington D.C., USA.

Diabetes is a disease with wide-ranging personal and societal impacts that has been managed medicinally for over half a century. Since the discovery of stem cells, pancreatic islet regeneration has become a central target for clinical application that has the potential to decrease or eliminate the need for insulin administration and adjunctive medications. The discovery of alternative routes to pluripotency that bypass the ethical implications of embryonic stem cells has significantly expanded the horizons of stem cell based therapy. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107653PMC
October 2016
5 Reads

Current progress of human trials using stem cell therapy as a treatment for diabetes mellitus.

Am J Stem Cells 2016 20;5(3):74-86. Epub 2016 Oct 20.

Georgetown University School of Medicine, Georgetown University Medical CenterWashington DC, USA; Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical CenterWashington DC, USA.

Diabetes mellitus affects millions of people worldwide, and is associated with serious complications that affect nearly all body systems. Because of the severity of this global health concern, there is a great deal of research being performed on alternative treatments and possible cures. Previous treatments for diabetes have included exogenous insulin injection and pancreatic islet transplantations. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107652PMC
October 2016
9 Reads

Human somatic cells acquire the plasticity to generate embryoid-like metamorphosis via the actin cytoskeleton in injured tissues.

Am J Stem Cells 2016 20;5(2):53-73. Epub 2016 Aug 20.

Pathology Department, Faculty of Medicine, University Cooperative of Colombia Villavicencio, Meta, Colombia.

Emergent biological responses develop via unknown processes dependent on physical collision. In hypoxia, when the tissue architecture collapses but the geometric core is stable, actin cytoskeleton filament components emerge, revealing a hidden internal order that identifies how each molecule is reassembled into the original mold, using one common connection, i.e. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043097PMC
August 2016
1 Read

CRISPR/Cas9 and mitochondrial gene replacement therapy: promising techniques and ethical considerations.

Am J Stem Cells 2016 20;5(2):39-52. Epub 2016 Aug 20.

School of Life Sciences, Arizona State University Tempe, AZ, USA.

Thousands of mothers are at risk of transmitting mitochondrial diseases to their offspring each year, with the most severe form of these diseases being fatal [1]. With no cure, transmission prevention is the only current hope for decreasing the disease incidence. Current methods of prevention rely on low mutant maternal mitochondrial DNA levels, while those with levels close to or above threshold (>60%) are still at a very high risk of transmission [2]. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043096PMC
August 2016
6 Reads

Pharmacological preconditioning for short-term ex vivo expansion of human umbilical cord blood hematopoietic stem cells by filgrastim.

Am J Stem Cells 2016 15;5(1):29-38. Epub 2016 May 15.

Genetics and IVF Unit, Department of Obstetrics and Gynaecology, Medical School, Ioannina University Gr 45110 Ioannina, Greece.

Although umbilical cord blood (UCB) hematopoietic stem cell transplantation (UCBT) has emerged as a promising haematological reconstitution therapy for leukemias and other related disorders, the insufficient UCB stem cell dosage still hinders better clinical outcomes. Previous research efforts, by focusing on ex vivo UCB expansion capabilities have sought to benefit from well-known mechanisms of self-renewal characteristics of UCB stem cells. However, the long-term (> 21 days) in vitro culture period and the low neutrophil recovery significantly reduce the transplantability of such ex vivo expanded UCB stem cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913295PMC
June 2016
8 Reads

Induction of sensory neurons from neuroepithelial stem cells by the ISX9 small molecule.

Am J Stem Cells 2016 15;5(1):19-28. Epub 2016 May 15.

Department of Neuroscience, Karolinska Institutet Retzius väg 8, SE-171 77, Stockholm, Sweden.

Hearing impairment most often involves loss of sensory hair cells and auditory neurons. As this loss is permanent in humans, a cell therapy approach has been suggested to replace damaged cells. It is thus of interest to generate lineage restricted progenitor cells appropriate for cell based therapies. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913294PMC
June 2016
7 Reads

Influence of paternal preconception exposures on their offspring: through epigenetics to phenotype.

Am J Stem Cells 2016 15;5(1):11-8. Epub 2016 May 15.

Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center Washington, DC, USA.

Historically, research into congenital defects has focused on maternal impacts on the fetal genome during gestation and prenatal periods. However, recent findings have sparked interest in epigenetic alterations of paternal genomes and its effects on offspring. This emergent field focuses on how environmental influences can epigenetically alter gene expression and ultimately change the phenotype and behavior of progeny. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913293PMC
June 2016
6 Reads

Comparison of stem cell therapies for acute kidney injury.

Am J Stem Cells 2016 15;5(1):1-10. Epub 2016 May 15.

Department of Biochemistry and Molecular and Cellular Biology, Georgetown University School of Medicine Washington, DC, USA.

Acute kidney injury (AKI) is the rapid onset of decreased kidney function that ultimately increases mortality and morbidity. Stem cell research is a promising avenue for curative and preventative therapies of kidney injury, however, there are many types of stem cells under investigation. Currently there is no research to compare the value of one stem cell method over another. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913292PMC
June 2016
3 Reads

The effect of autologous bone marrow mononuclear cell transplantation on the survival duration in Amyotrophic Lateral Sclerosis - a retrospective controlled study.

Am J Stem Cells 2015 15;4(1):50-65. Epub 2015 Mar 15.

NeuroGen Brain and Spine Institute, Stemasia Hospital and Research Center Plot No. 19, Next to Seawood Grand Central Station (West), off Palm Beach Road, Navi Mumbai 400706, Maharashtra, India.

Amyotrophic Lateral Sclerosis (ALS) is a rapidly progressive neurodegenerative disorder with fatal prognosis. Cellular therapy has been studied for ALS in various animal models and these advances have highlighted its potential to be a treatment modality. This is a retrospective controlled cohort study of total 57 patients. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396155PMC
May 2015
6 Reads

An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.

Am J Stem Cells 2015 15;4(1):38-49. Epub 2015 Mar 15.

Research and Development Center for Cell Therapy, Foundation for Biomedical Research and Innovation TRI#308, 1-5-4 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0043, Japan.

Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control of cells at single cell level. Here we report a robust single cell freezing and thawing method of hPSCs cultured in chemically-defined medium TeSR(TM)-E8(TM) and on cost-effective recombinant human Vitronectin-N (rhVTN-N)-coated dish. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396159PMC
May 2015
2 Reads
1 Citation

Experimental approaches to derive CD34+ progenitors from human and nonhuman primate embryonic stem cells.

Am J Stem Cells 2015 15;4(1):32-7. Epub 2015 Mar 15.

Southwest National Primate Research Center, Texas Biomedical Research Institute San Antonio, Texas, 78227-5301 ; South Texas Diabetes and Obesity Institute, University of Texas Health Science Center, San Antonio - Regional Academic Health Center 80 Fort Brown Street, Brownsville, Texas 78520.

Traditionally, CD34 positive cells are predominantly found in the umbilical cord and bone marrow, thus are considered as hematopoietic progenitors. Increasing evidence has suggested that the CD34+ cells represent a distinct subset of cells with enhanced progenitor activity; CD34 is a general marker of progenitor cells in a variety of cell types. Because the CD34 protein shows expression early on in hematopoietic and vascular-associated tissues, CD34+ cells have enormous potential as cellular agents for research and for clinical cell transplantation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396158PMC
May 2015
1 Read

Stem cells for the cell and molecular therapy of type 1 diabetes mellitus (T1D): the gap between dream and reality.

Am J Stem Cells 2015 15;4(1):22-31. Epub 2015 Mar 15.

Department of Medicine, Section of Clinical Cardiovascular, Endocrine and Metabolic Physiology, University of Perugia School of Medicine at Terni Terni, Italy.

In spite of intense research, over the past 2-3 decades, targeted to validating methods for the cure of T1D, based on cell substitution therapy in the place of exogenously administered insulin injections, achievement of the final goal continues to remain out of reach. In fact, aside of very limited clinical success of the few clinical trials of pancreatic islet cell transplantation in totally immunosuppressed patients with T1D, the vast majority of these diabetic patients invariably is insulin-dependent. New advances for cell and molecular therapy for T1D, including use of stem cells, are reviewed and discussed in an attempt to clearly establish where we are and where are we may go for the final cure for T1DM. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396156PMC
May 2015
3 Reads

Transdifferentiation of endothelial cells to smooth muscle cells play an important role in vascular remodelling.

Am J Stem Cells 2015 15;4(1):13-21. Epub 2015 Mar 15.

Department of Pulmonary Medicine, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona Barcelona 08036, Spain ; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias Spain.

Pulmonary artery remodelling it is a major feature of pulmonary hypertension (PH). It is characterised by cellular and structural changes of the pulmonary arteries causing higher pulmonar vascular resistance and right ventricular failure. Abnormal deposition of smooth muscle-like (SM-like) cells in normally non-muscular, small diameter vessels and a deregulated control of endothelial cells are considered pathological features of PH. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396157PMC
May 2015
4 Reads

Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine.

Am J Stem Cells 2015 15;4(1):1-12. Epub 2015 Mar 15.

Department of Veterinary Clinical Sciences, Small Animal Clinic c/o Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen Frankfurter Str. 98. 35394, Giessen, Germany.

Adipose tissue derived stem cells (ASCs) are mesenchymal stem cells which can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, which is easy accessible and the cells can be obtained in large numbers, cultivated and expanded in vitro and prepared for tissue engineering approaches, especially for skeletal tissue repair. In the recent years this cell population has attracted a great amount of attention among researchers in human as well as in veterinary medicine. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396154PMC
May 2015
26 Reads

Hematopoietic stem cells derived from human umbilical cord ameliorate cisplatin-induced acute renal failure in rats.

Am J Stem Cells 2014 5;3(2):83-96. Epub 2014 Sep 5.

Department of Zoology, faculty of Women's, Ain Shams University Egypt.

Injury to a target organ can be sensed by bone marrow stem cells that migrate to the site of damage, undergo differentiation, and promote structural and functional repair. This remarkable stem cell capacity prompted an investigation of the potential of mesenchymal and hematopoietic stem cells to cure acute renal failure. On the basis of the recent demonstration that hematopoietic stem cells (HSCs) can differentiate into renal cells, the current study tested the hypothesis that HSCs can contribute to the regeneration of renal tubular epithelial cells after renal injury. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163607PMC
September 2014
3 Reads

The Oct4 protein: more than a magic stemness marker.

Am J Stem Cells 2014 5;3(2):74-82. Epub 2014 Sep 5.

Univ. Bordeaux, CIRID, UMR 5164 F-33 000 Bordeaux, France ; CNRS, CIRID, UMR 5164 F-33 000 Bordeaux, France.

The Oct4 protein, encoded by the Pou5f1 gene was the very first master gene, discovered 25 years ago, to be absolutely required for the stemness properties of murine and primate embryonic stem cells. This transcription factor, which has also been shown to be essential for somatic cell reprogrammation, displays various functions depending upon its level of expression and has been quoted as a "rheostat" gene. Oct4 protein is in complexes with many different partners and its activity depends upon fine post-translational modifications. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163606PMC
September 2014
5 Reads

Emerging paradigms of long non-coding RNAs in gastrointestinal cancer.

Am J Stem Cells 2014 5;3(2):63-73. Epub 2014 Sep 5.

Department of Pathology, School of Basic Medical Sciences, Southern Medical University Guangzhou 510515, China.

A large number of long non-coding RNAs (lncRNAs) have been discovered by genome-wide transcriptional analyses. Emerging evidence has indicated that lncRNAs regulate gene expression at epigenetic, transcription, and post-transcription levels, are widely involved in various pathobiology of human diseases, and may play an important role in the biology of cancer stem cells. Alterations of specific lncRNAs have been revealed to interact with the major pathways of cell proliferation, apoptosis, differentiation, invasion and metastasis in many human malignancies, such as gastrointestinal cancer. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163605PMC
September 2014
5 Reads

Metastatic cancer stem cells: from the concept to therapeutics.

Am J Stem Cells 2014 5;3(2):46-62. Epub 2014 Sep 5.

Department of Pathology, School of Basic Medical Sciences, Southern Medical University Guangzhou 510515, China.

Metastatic cancer stem cells (MCSCs) refer to a subpopulation of cancer cells with both stem cell properties and invasion capabilities that contribute to cancer metastasis. MCSCs have capability of self-renewal, potentials of multiple differentiation and development and/or reconstruction of cancer tissues. As compared with stationary cancer stem cells, MCSCs are capable of invasion to normal tissues such as vasculatures, resistance to chemo- and/or radio-therapies, escape from immune surveillance, survival in circulation and formation of metastasis. Read More

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http://www.ajsc.us/files/ajsc0001488.pdf
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163604PMC
September 2014
8 Reads

Phenotype-dependent role of the L-type calcium current in embryonic stem cell derived cardiomyocytes.

Am J Stem Cells 2014 13;3(1):37-45. Epub 2014 Mar 13.

Cardiovascular Sciences, Child and Family Research Institute Vancouver, BC, Canada ; Molecular Cardiac Physiology Group, Simon Fraser University Burnaby, BC, Canada.

Although the L-type Ca(2+) current (ICa,L) plays an important role in cardiac contractility and pacemaking, its role in embryonic stem-cell derived cardiomyocytes (ESC-CMs) has not yet been explored in detail. We used patch-clamp techniques to characterize ICa,L, action potential properties, and nifedipine (an ICa,L blocker) sensitivity on spontaneously contracting embryoid bodies (EBs) or isolated ESC-CMs. Cellular preparations exhibited differential sensitivity to nifedipine, with substantial variation in the dose required to abolish automaticity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960756PMC
March 2014
3 Reads

Rapid isolation of integrin rich multipotent stem cell pool and reconstruction of mouse epidermis equivalent.

Am J Stem Cells 2014 13;3(1):27-36. Epub 2014 Mar 13.

Department of Biotechnology, Integral University Lucknow-226026, India.

We describe here epidermis reconstruction using multipotent mouse epidermal stem cells (EpSCs) enriched from keratinocyte isolates exploting exclusively the stem cell-adhesive property. This method excluded flowcytometry and was swift. Percent enrichment was measured by the uptake of Propidium iodide and Hoechst-33342 dye using flowcytometry to determine EpSCs yield. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960755PMC
March 2014
6 Reads

Effect of transplantation of human embryonic stem cell-derived neural progenitor cells on adult neurogenesis in aged hippocampus.

Am J Stem Cells 2014 13;3(1):21-6. Epub 2014 Mar 13.

Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine Baltimore, Maryland, USA.

Adult neurogenesis occurs within the special microenvironment in the subgranular zone of the hippocampus and the subventricular zone of the lateral ventricle of the mammalian brain. The special microenvironment is known as neurogenic niches. Multiple cell types, including endothelial cells, astroglia, ependymal cells, immature progeny of neural stem cells, and mature neurons, comprise the neurogenic niche. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960754PMC
June 2014
11 Reads

Decellularized matrices for cardiovascular tissue engineering.

Am J Stem Cells 2014 13;3(1):1-20. Epub 2014 Mar 13.

Cardiovascular Research Center, Yale Medical School New Haven, CT, 06511, US.

Cardiovascular disease (CVD) is one of the leading causes of death in the Western world. The replacement of damaged vessels and valves has been practiced since the 1950's. Synthetic grafts, usually made of bio-inert materials, are long-lasting and mechanically relevant, but fail when it comes to "biointegration". Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960753PMC
June 2014
2 Reads

Activation and crosstalk between TNF family receptors in umbilical cord blood cells is not responsible for loss of engraftment capacity following culture.

Am J Stem Cells 2013 22;2(3):155-64. Epub 2013 Dec 22.

Frankel Laboratory, Center for Stem Cell Research, Department of Pediatric Hematology-Oncology, Schneider Children's Medical Center of Israel Petach Tikva 49202, Israel.

Umbilical cord blood (UCB) is a rich source of hematopoietic progenitors for transplantation. Murine and human progenitors are insensitive to apoptotic signaling mediated by the TNF family receptors, however extension of culture over 48 hours is accompanied by severe deterioration in engraftment and hematopoietic reconstituting capacity. In this study we assessed crosstalk between the Fas, TNF and TRAIL receptors, and questioned whether it contributes to increased mortality and decreased activity of UCB progenitors following extended ex vivo culture for 72 hours. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875276PMC
January 2014
2 Reads

Therapeutic potential of Pnmt+ primer cells for neuro/myocardial regeneration.

Am J Stem Cells 2013 Dec 22;2(3):137-54. Epub 2013 Dec 22.

Burnett School of Biomedical Sciences, University of Central Florida College of Medicine Orlando, FL 32827, USA.

Phenylethanolamine n-methyltransferase (Pnmt) catalyzes the conversion of norepinephrine into epinephrine, and thus serves as a marker of adrenergic cells. In adults, adrenergic cells are present in the adrenal medullae and the central and peripheral (sympathetic) nervous systems where they play key roles in stress responses and a variety of other functions. During early embryonic development, however, Pnmt first appears in the heart where it is associated with specialized myocytes in the pacemaking and conduction system. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875277PMC
December 2013

Pancytopenia related to dental adhesive in a young patient.

Am J Stem Cells 2013 30;2(2):132-6. Epub 2013 Jun 30.

Hematology/Oncology Mary Babb Randolph Cancer Center, West Virginia University Morgantown, WV, USA.

Copper deficiency resulting in hypocupremia is a rare cause of pancytopenia associated with a neurological syndrome. Hypocupremia may also occur as a consequence of excessive oral zinc consumption as described by Brewer et al and several other groups. Dental fixatives have been described as a potential source of hyperzincemia in patients. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708507PMC
July 2013
2 Reads

A robust method to derive functional neural crest cells from human pluripotent stem cells.

Am J Stem Cells 2013 30;2(2):119-31. Epub 2013 Jun 30.

Gladstone Institute of Cardiovascular Disease 1650 Owens Street, San Francisco, CA 94158, USA.

Neural crest (NC) cells contribute to the development of many complex tissues of all three germ layers during embryogenesis, and its abnormal development accounts for several congenital birth defects. Generating NC cells-including specific subpopulations such as cranial, cardiac, and trunk NC cells-from human pluripotent stem cells will provide a valuable model system to study human development and disease. Here, we describe a rapid and robust NC differentiation method called "LSB-short" that is based on dual SMAD pathway inhibition. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708511PMC
July 2013
8 Reads

Amniotic fluid derived stem cells give rise to neuron-like cells without a further differentiation potential into retina-like cells.

Am J Stem Cells 2013 30;2(2):108-18. Epub 2013 Jun 30.

Institute of Veterinary-Anatomy, Histology and Embryology, Justus-Liebig University Giessen Germany.

Amniotic fluid contains heterogeneous cell types and has become an interesting source for obtaining fetal stem cells. These stem cells have a high proliferative capacity and a good differentiation potential and may thus be suitable for regenerative medicine. As there is increasing evidence, that these stem cells are also able to be directed into the neural lineage, in our study we investigated the neuronal and glial differentiation potential of these cells, so that they may also be applied to cure degenerative diseases of the retina. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708508PMC

The development of hematopoietic and mesenchymal stem cell transplantation as an effective treatment for multiple sclerosis.

Am J Stem Cells 2013 30;2(2):95-107. Epub 2013 Jun 30.

Department of Biochemistry and Molecular & Cellular Biology, Georgetown University School of Medicine 3900 Reservoir Rd. NW, Washington DC 20057, USA.

This article examines the current use and future implications of stem cell therapy in treating Multiple Sclerosis (MS). MS is the most common neurological disease in young adults, affecting approximately two million people worldwide. Currently there is no cure for MS. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708509PMC
July 2013
1 Read

On becoming neural: what the embryo can tell us about differentiating neural stem cells.

Am J Stem Cells 2013 30;2(2):74-94. Epub 2013 Jun 30.

Department of Anatomy and Regenerative Biology, The George Washington University, School of Medicine and Health Sciences 2300 I (eye) Street, N.W., Washington, D.C. 20037, USA ; Institute for Neuroscience, The George Washington University, School of Medicine and Health Sciences 2300 I (eye) Street, N.W., Washington, D.C. 20037, USA.

THE EARLIEST STEPS OF EMBRYONIC NEURAL DEVELOPMENT ARE ORCHESTRATED BY SETS OF TRANSCRIPTION FACTORS THAT CONTROL AT LEAST THREE PROCESSES: the maintenance of proliferative, pluripotent precursors that expand the neural ectoderm; their transition to neurally committed stem cells comprising the neural plate; and the onset of differentiation of neural progenitors. The transition from one step to the next requires the sequential activation of each gene set and then its down-regulation at the correct developmental times. Herein, we review how these gene sets interact in a transcriptional network to regulate these early steps in neural development. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708510PMC
July 2013
1 Read

Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro.

Am J Stem Cells 2013 8;2(1):62-73. Epub 2013 Mar 8.

Institute of Veterinary -Anatomy, -Histology, and -Embryology, Justus-Liebig University of Giessen Germany.

Mesenchymal stem cells are regarded as common cellular precursors of the musculoskeletal tissue and are responsible for tissue regeneration in the course of musculoskeletal disorders. In equine veterinary medicine extracorporeal shock wave therapy (ESWT) is used to optimize healing processes of bone, tendon and cartilage. Nevertheless, little is known about the effects of the shock waves on cells and tissues. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636727PMC
May 2013
1 Read

Cancer stem cells and tumor transdifferentiation: implications for novel therapeutic strategies.

Am J Stem Cells 2013 8;2(1):52-61. Epub 2013 Mar 8.

University of Wisconsin School of Medicine & Public Health, Department of Dermatology Madison, Wisconsin 53706, USA.

Highly malignant tumors mostly consist of rapidly proliferating cells. However, tumors also contain a few cells in a quiescent state that can be characterized as slow-cycling, expressing markers of stem cells and possessing the ability to initiate new tumors. These quiescent cells, now generally termed 'cancer stem cells' (CSC) (or 'cancer initiating cells'), are capable of regenerating the entire tumor--as it occurs in metastatic spread. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636725PMC
May 2013
5 Reads

Genetic instability of modified stem cells - a first step towards malignant transformation?

Am J Stem Cells 2013 8;2(1):39-51. Epub 2013 Mar 8.

Institute of Cell and Molecular Pathology, Hannover Medical School Hannover, Germany.

Induced pluripotent stem cells (iPSC) are important tools in regenerative medicine. Yet, it is becoming increasingly clear that the reprogramming process, including retroviral transduction with potent oncogenes like c-Myc and long-term cultivation, may induce genetic instability. Genetically altered iPS cells can grow out and dominate the cell culture. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636728PMC
May 2013
43 Reads

Mesenchymal stem cell and regenerative medicine: regeneration versus immunomodulatory challenges.

Am J Stem Cells 2013 8;2(1):22-38. Epub 2013 Mar 8.

Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine 108 C R Avenue, Kolkata-700073, India.

Mesenchymal Stem cells (MSC) are now presented with the opportunities of multifunctional therapeutic approaches. Several reports are in support of their self-renewal, capacity for multipotent differentiation, and immunomodulatory properties. They are unique to contribute to the regeneration of mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, and adipose. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636724PMC

BMP9 signaling in stem cell differentiation and osteogenesis.

Am J Stem Cells 2013 8;2(1):1-21. Epub 2013 Mar 8.

Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center Chicago, IL 60637, USA.

Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily and play a critical role in skeletal development, bone formation and stem cell differentiation. Disruptions in BMP signaling result in a variety of skeletal and extraskeletal anomalies. BMP9 is a poorly characterized member of the BMP family and is among the most osteogenic BMPs, promoting osteoblastic differentiation of mesenchymal stem cells (MSCs) both in vitro and in vivo. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636726PMC
May 2013
48 Reads