6,465 results match your criteria American journal of physiology. Renal physiology[Journal]


Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.

Am J Physiol Renal Physiol 2020 Jul 6. Epub 2020 Jul 6.

Renal Research Laboratory, Kansas City VA Medical Center.

The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes. Hyperfiltration-associated increase in FFSS can lead to podocyte injury and detachment. Previously, we showed that FFSS-induced upregulation of the COX2-PGE2-EP2 axis in podocytes activates the Akt-GSK3β-β-catenin and MAPK/ERK signaling in response to FFSS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00183.2020DOI Listing

MiR-21 promotes calcium oxalate-induced renal tubular cell injury by targeting PPARA.

Am J Physiol Renal Physiol 2020 Jul 6. Epub 2020 Jul 6.

Department of Urology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, China.

Kidney stone disease is a crystal concretion formed in kidneys which has been associated with an increased risk of chronic kidney disease. MicroRNAs are functionally involved in kidney injury. Data mining using a microRNA array database suggested that miR-21 may be associated with calcium oxalate monohydrate (COM)-induced renal tubular cell injury. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00132.2020DOI Listing

Aldosterone-dependent and -independent regulation of Na and K excretion and ENaC in mouse kidney.

Am J Physiol Renal Physiol 2020 Jul 6. Epub 2020 Jul 6.

Physiology and Biophysics, Weill-Cornell Medical College, United States.

We investigated the regulation of Naand K excretion and ENaC in mice lacking the gene for aldosterone synthase (AS), using clearance methods to assess excretion and electrophysiology and Western blotting to test for ENaC activity and processing. After one day of dietary Na restriction AS-/- mice lost more Na in the urine than did AS+/+. After 1 week on this diet both genotypes strongly reduced urinary Na excretion, but creatinine clearance decreased only in AS-/- mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00204.2020DOI Listing

Urinary bladder smooth muscle ion channels: expression, function, and regulation in health and disease.

Am J Physiol Renal Physiol 2020 Jul 6. Epub 2020 Jul 6.

Pharmaceutical Sciences, Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, Tennessee, United States.

The urinary bladder smooth muscle (UBSM), also known as detrusor smooth muscle, forms the bladder wall and ultimately determines the two main attributes of the organ: urine storage and voiding. The two functions are facilitated by UBSM relaxation and contraction, respectively, which depend on UBSM excitability shaped by multiple ion channels. In this review, we summarize the current understanding of key ion channels establishing and regulating UBSM excitability and contractility. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00048.2020DOI Listing

Gene expression profiling of proximal and distal renal tubules in Atlantic salmon (Salmo salar) acclimated to fresh water and seawater.

Am J Physiol Renal Physiol 2020 Jul 6. Epub 2020 Jul 6.

Department of Clinical Genetics, Odense University Hospital, Denmark.

Euryhaline teleost kidneys undergo a major functional switch from being filtratory in fresh water (FW) to being predominantly secretory in seawater (SW) conditions. The transition involves both vascular and tubular effects. There is consensus that glomerular filtration rate is greatly reduced upon exposure to hyperosmotic conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00557.2019DOI Listing

Hypertensive Female Sprague Dawley rats require an intact nitric oxide synthase system for compensatory increases in renal T regulatory cells.

Am J Physiol Renal Physiol 2020 Jun 29. Epub 2020 Jun 29.

Physiology, Augusta University, United States.

We have previously shown that hypertensive female rats have more T regulatory cells (Tregs) which contribute more to blood pressure (BP) control in females vs males. Based on known protective properties of Tregs, the goal of this study was to investigate the mechanisms by which females maintain Tregs. The current study was designed to 1) compare the impact of three hypertension models on the percentage of renal Tregs and 2) test the hypothesis that NOS inhibition prevents increases in renal Tregs and exacerbates renal damage in female Sprague Dawley (SD) rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00228.2020DOI Listing

A two-stage bilateral ischemia reperfusion injury-induced AKI to CKD transition model in mice.

Am J Physiol Renal Physiol 2020 Jun 22. Epub 2020 Jun 22.

Molecular Pharmacology and Physiology, University of South Flroida College of Medicine, United States.

Acute kidney injury (AKI) significantly increases the risk of the development of chronic kidney disease (CKD). Recently, our laboratory generated a mouse model with the typical phenotypes of AKI to CKD transition in unilateral kidney. However, AKI, CKD, and even the transition from AKI to CKD usually occur in bilateral kidneys rather than unilateral kidney in patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00017.2020DOI Listing

RAB-RABPHILIN SYSTEM IN INJURED HUMAN PODOCYTES STRESSED BY GLUCOSE OVERLOAD AND ANGIOTENSIN II.

Am J Physiol Renal Physiol 2020 Jun 22. Epub 2020 Jun 22.

Genomic and Genetic Diagnosis, Biomedical Research Institute INCLIVA, Spain.

Kidney injury in hypertension and diabetes entails, among in other structures, damage in a key cell of the glomerular filtration barrier, the podocyte. Podocytes are polarized and highly differentiated cells in which vesicular transport, partly driven by Rab GTPases, is a relevant process. The present aim was to analyze Rab GTPases of the Rab-Rabphilin system in human immortalized podocytes and the impact of high glucose and angiotensin II. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00077.2020DOI Listing

Quantitative intravital calcium imaging maps single cell behavior to kidney tubular structure.

Am J Physiol Renal Physiol 2020 Jun 22. Epub 2020 Jun 22.

Anatomy, University of Zurich, Switzerland.

Ca is an important second messenger that translates extracellular stimuli into intracellular responses. Although there has been significant progress in understanding Ca dynamics in organs such as the brain, the nature of Ca signals in the kidney is still poorly understood. Here, we show that by using a genetically expressed highly sensitive reporter (GCaMP6s) it is possible to perform imaging of Ca signals at high resolution in the mouse kidney in vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00052.2020DOI Listing

Sex differences in renal ischemia/reperfusion injury.

Am J Physiol Renal Physiol 2020 Jun 22. Epub 2020 Jun 22.

Ist Department of Pediatrics, Semmelweis University, Hungary.

Ischemia/reperfusion injury of the kidney is caused by the sudden and temporary obstruction of blood flow to the organ. Renal ischemia/reperfusion injury is associated with high morbidity and mortality, but effective therapies are lacking. Sexual dimorphism in renal injury has been acknowledged since the 1940s and the possible role of sex hormones has been intensively investigated in the past decades. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00099.2020DOI Listing

Molecular targeting of renal inflammation using drug delivery technology to inhibit NF-κB improves renal recovery in chronic kidney disease.

Am J Physiol Renal Physiol 2020 Jul 15;319(1):F139-F148. Epub 2020 Jun 15.

Department of Neurology, University of Mississippi Medical Center, Jackson, Mississippi.

Inflammation is a major determinant for the progression of chronic kidney disease (CKD). NF-κB is a master transcription factor upregulated in CKD that promotes inflammation and regulates apoptosis and vascular remodeling. We aimed to modulate this pathway for CKD therapy in a swine model of CKD using a peptide inhibitor of the NF-κB p50 subunit (p50i) fused to a protein carrier [elastin-like polypeptide (ELP)] and equipped with a cell-penetrating peptide (SynB1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00155.2020DOI Listing

Evolution of Altered Tubular Metabolism and Mitochondrial Function in Sepsis Associated Acute Kidney Injury.

Am J Physiol Renal Physiol 2020 Jun 15. Epub 2020 Jun 15.

Medicine-Nephrology, UC San Diego and VASDHS, United States.

Sepsis-associated acute kidney injury (s-AKI) has a staggering impact in patients and lacks any treatment. Incomplete understanding of the pathogenesis of s-AKI is a major barrier to the development of effective therapies. We address the gaps in knowledge regarding renal oxygenation, tubular metabolism, and mitochondrial function in the pathogenesis of s-AKI, utilizing the cecal ligation and puncture (CLP) model in mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00390.2019DOI Listing

Chronic unilateral cervical vagotomy reduces renal inflammation, blood pressure, and renal injury in a mouse model of lupus.

Am J Physiol Renal Physiol 2020 Jun 15. Epub 2020 Jun 15.

Physiology and Anatomy, University of North Texas Health Science Center, United States.

Background: Systemic lupus erythematosus (SLE) is characterized by hypertension that results from chronic renal inflammation and dysautonomia in the form of dampened vagal tone. In health, the vagus nerve regulates inflammatory processes through mechanisms like the cholinergic anti-inflammatory pathway; so in the case of SLE, reduced efferent vagus nerve activity may indirectly affect renal inflammation, and therefore hypertension. In this study, we sought to investigate the impact of disrupting vagal neurotransmission on renal inflammation and hypertension in the setting of chronic inflammatory disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00201.2020DOI Listing

An acute rise of plasma sodium concentration associates with syndecan-1 shedding during hemodialysis.

Am J Physiol Renal Physiol 2020 Jun 15. Epub 2020 Jun 15.

Nephrology, University Medical Center Groningen, Netherlands.

Endothelial dysfunction (ED) contributes to the high incidence of cardiovascular events in hemodialysis patients. Syndecan-1 in the endothelial glycocalyx can be shed into the circulation serving as a biomarker for ED. As sodium is a trigger for glycocalyx shedding, we now tested whether hemodialysis with higher dialysate sodium concentrations is associated with more syndecan-1 shedding compared with standard hemodialysis (SHD). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00005.2020DOI Listing

1,3-Butanediol attenuates hypertension and suppresses kidney injury in female rats.

Am J Physiol Renal Physiol 2020 Jul 8;319(1):F106-F114. Epub 2020 Jun 8.

Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.

Thirty-seven million people in the United States are estimated to have chronic kidney disease (CKD). Hypertension (HTN) is the second leading risk factor for developing kidney disease. A recent study reported that increasing levels of β-hydroxybutyrate levels by administration of its precursor, 1,3-butanediol, decreased salt-induced HTN in male Dahl salt-sensitive (S) rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00141.2020DOI Listing

Ultrasound for the treatment of acute kidney injury and other inflammatory conditions: a promising path toward noninvasive neuroimmune regulation.

Am J Physiol Renal Physiol 2020 Jul 8;319(1):F125-F138. Epub 2020 Jun 8.

Division of Nephrology and Center for Immunity, Inflammation, and Regenerative Medicine, University of Virginia, Charlottesville, Virgnia.

Acute kidney injury (AKI) is an important clinical disorder with high prevalence, serious consequences, and limited therapeutic options. Modulation of neuroimmune interaction by nonpharmacological methods is emerging as a novel strategy for treating inflammatory diseases, including AKI. Recently, pulsed ultrasound (US) treatment was shown to protect from AKI by stimulating the cholinergic anti-inflammatory pathway. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00145.2020DOI Listing

A noninvasive method to study the evolution of extracellular fluid volume in mice using time-domain nuclear magnetic resonance.

Am J Physiol Renal Physiol 2020 Jul 1;319(1):F115-F124. Epub 2020 Jun 1.

North Florida/South Georgia Veterans Health System, Gainesville, Florida.

Maintaining water homeostasis is fundamental for cellular function. Many diseases and drugs affect water balance and plasma osmolality. Water homeostasis studies in small animals require the use of invasive or terminal methods that make intracellular fluid volume and extracellular fluid volume (ECF) monitoring over time stressful and time consuming. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00377.2019DOI Listing

Long noncoding RNA NEAT1 sponges miR-129 to modulate renal fibrosis by regulation of collagen type I.

Am J Physiol Renal Physiol 2020 Jul 1;319(1):F93-F105. Epub 2020 Jun 1.

Department of Nephrology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, People's Republic of China.

The long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1) has been reported to promote liver fibrosis progression. However, its molecular mechanism in renal fibrosis was not elucidated. In the present study, an in vitro model of renal fibrosis was established with HK-2 and HKC-8 cells treated with transforming growth factor-β1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00552.2019DOI Listing

IN VITRO EFFICACY AND SAFETY OF A SYSTEM FOR SORBENT ASSISTED PERITONEAL DIALYSIS.

Am J Physiol Renal Physiol 2020 Jun 1. Epub 2020 Jun 1.

Pathology, University Medical Center Utrecht, Netherlands.

Background: A system for sorbent assisted peritoneal dialysis (SAPD) was designed to continuously recirculate dialysate via a tidal mode using a single lumen peritoneal catheter with regeneration of spent dialysate by means of sorbent technology. We hypothesize that SAPD treatment will maintain a high plasma-to-dialysate concentration gradient and increase the mass transfer area coefficient of solutes. Thereby, the SAPD system may enhance clearance while reducing the number of exchanges. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00079.2020DOI Listing

Chronic elevation of renal venous pressure induces extensive renal venous collateral formation and modulates renal function and cardiovascular stability in rats.

Am J Physiol Renal Physiol 2020 Jul 1;319(1):F76-F83. Epub 2020 Jun 1.

Department of Medicine, University of Alberta, Edmonton, Alberta, Canada.

Acutely increased renal venous pressure (RVP) impairs renal function, but the long-term impact is unknown. We investigated whether chronic RVP elevation impairs baseline renal function and prevents exacerbation of renal dysfunction and cardiovascular instability upon further RVP increase. RVP elevation (20-25 mmHg) or sham operation (sham) was performed in rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00542.2019DOI Listing

Pharmacokinetics and pharmacodynamics of TTI-101, a STAT3 inhibitor that blocks muscle proteolysis in rats with chronic kidney disease.

Am J Physiol Renal Physiol 2020 Jul 1;319(1):F84-F92. Epub 2020 Jun 1.

Nephrology Division, Department of Medicine, Baylor College of Medicine, Houston, Texas.

Loss of muscle proteins increases the morbidity and mortality of patients with chronic kidney disease (CKD), and there are no reliable preventive treatments. We uncovered a STAT3/CCAAT-enhancer-binding protein-δ to myostatin signaling pathway that activates muscle protein degradation in mice with CKD or cancer; we also identified a small-molecule inhibitor of STAT3 (TTI-101) that blocks this pathway. To evaluate TTI-101 as a treatment for CKD-induced cachexia, we measured TTI-101 pharmacokinetics and pharmacodynamics in control and CKD rats that were orally administered TTI-101or its diluent. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00603.2019DOI Listing

Normal-range urinary albumin excretion associates with blood pressure and renal electrolyte handling in pregnancy.

Am J Physiol Renal Physiol 2020 Jul 28;319(1):F1-F7. Epub 2020 May 28.

Institute for Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Albuminuria in the pathological range is a significant predictor of preeclampsia. In healthy persons, high normal urinary albumin predicts a later incidence of hypertension and is associated with salt sensitivity of blood pressure. We hypothesized that in pregnancy urinary albumin in the normal range associates with blood pressure through activation of distal Na reabsorption and renal salt retention by plasma factors cofiltered with albumin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00044.2020DOI Listing

Renal ischemia alters expression of mitochondria-related genes and impairs mitochondrial structure and function in swine scattered tubular-like cells.

Am J Physiol Renal Physiol 2020 Jul 28;319(1):F19-F28. Epub 2020 May 28.

Division of Nephrology and Hypertension, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota.

Scattered tubular-like cells (STCs) are dedifferentiated surviving tubular epithelial cells that repair neighboring injured cells. Experimental renal artery stenosis (RAS) impairs STC reparative potency by inducing mitochondrial injury, but the exact mechanisms of mitochondrial damage remain unknown. We hypothesized that RAS alters expression of mitochondria-related genes, contributing to mitochondrial structural damage and dysfunction in swine STCs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00120.2020DOI Listing

NF-κB blockade during short-term L-NAME and salt overload strongly attenuates the late development of chronic kidney disease.

Am J Physiol Renal Physiol 2020 May 28. Epub 2020 May 28.

University of São Paulo.

NO synthase inhibition by L-NAME plus high-salt diet (HS) is a model of Chronic Kidney Disease (CKD) characterized by marked hypertension and renal injury. With cessation of treatment, most of these changes subside, but progressive renal injury develops, associated with persistent low-grade renal inflammation. We investigated whether innate immunity, and in particular the NF-kB system, is involved in this process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00495.2019DOI Listing

Differential effects of low-dose sacubitril and/or valsartan on renal disease in salt-sensitive hypertension.

Am J Physiol Renal Physiol 2020 Jul 28;319(1):F63-F75. Epub 2020 May 28.

Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina.

Diuretics and renin-angiotensin system blockers are often insufficient to control the blood pressure (BP) in salt-sensitive (SS) subjects. Abundant data support the proposal that the level of atrial natriuretic peptide may correlate with the pathogenesis of SS hypertension. We hypothesized here that increasing atrial natriuretic peptide levels with sacubitril, combined with renin-angiotensin system blockage by valsartan, can be beneficial for alleviation of renal damage in a model of SS hypertension, the Dahl SS rat. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00125.2020DOI Listing

14-3-3γ, a novel regulator of the large-conductance Ca-activated K channel.

Am J Physiol Renal Physiol 2020 Jul 28;319(1):F52-F62. Epub 2020 May 28.

Renal Divison, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia.

14-3-3γ is a small protein regulating its target proteins through binding to phosphorylated serine/threonine residues. Sequence analysis of large-conductance Ca-activated K (BK) channels revealed a putative 14-3-3 binding site in the COOH-terminal region. Our previous data showed that 14-3-3γ is widely expressed in the mouse kidney. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00584.2019DOI Listing

Novel catheter design enables transurethral catheterization of male mice.

Am J Physiol Renal Physiol 2020 Jul 28;319(1):F29-F32. Epub 2020 May 28.

Children's National Hospital, Washington, District of Columbia.

The male mouse is underrepresented in research of the urinary tract due to the difficulty of transurethral catheterization. As a result, there is a lack of analysis of sex differences in urinary tract research. Here, we present a novel catheter design and technique that enables urethral catheterization of male mice for bladder inoculation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00121.2020DOI Listing

Sex differences in endothelial function in chronic kidney disease.

Am J Physiol Renal Physiol 2020 Jul 18;319(1):F33-F40. Epub 2020 May 18.

Division of Nephrology, Department of Internal Medicine, Carver College of Medicine, Iowa City, Iowar.

Vascular dysfunction plays an important role in the etiology of chronic kidney disease (CKD) and is associated with cardiovascular diseases. Sex differences in vascular function are common in clinical and nonclinical populations. However, no data exist in individuals with CKD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00156.2020DOI Listing

Monophosphoryl lipid A pretreatment suppresses sepsis- and LPS-induced proinflammatory cytokine production in the medullary thick ascending limb.

Am J Physiol Renal Physiol 2020 Jul 18;319(1):F8-F18. Epub 2020 May 18.

Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas.

Sepsis is the leading cause of acute kidney injury in critically ill patients. Tumor necrosis factor-α (TNF-α) has been implicated in the pathogenesis of septic kidney injury; however, the sites and mechanisms of renal TNF-α production during sepsis remain to be defined. In the present study, we showed that TNF-α expression is increased in medullary thick ascending limbs (MTALs) of mice with sepsis induced by cecal ligation and puncture. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00178.2020DOI Listing

Is the kidney a target of SARS-CoV-2?

Am J Physiol Renal Physiol 2020 06 15;318(6):F1454-F1462. Epub 2020 May 15.

Molecular Physiology Unit, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, México.

The new disease produced by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) represents a major pandemic event nowadays. Since its origin in China in December 2019, there is compelling evidence that novel SARS-CoV-2 is a highly transmissible virus, and it is associated to a broad clinical spectrum going from subclinical presentation to severe respiratory distress and multiorgan failure. Like other coronaviruses, SARS-CoV-2 recognizes human angiotensin-converting enzyme 2 as a cellular receptor that allows it to infect different host cells and likely disrupts renin-angiotensin-aldosterone system homeostasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00160.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303722PMC

Tensin2 is important for podocyte-glomerular basement membrane interaction and integrity of the glomerular filtration barrier.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1520-F1530. Epub 2020 May 11.

Department of Human Sciences, Osaka International University, Moriguchi, Osaka, Japan.

Tensin2 (Tns2), an integrin-linked protein, is enriched in podocytes within the glomerulus. Previous studies have revealed that -deficient mice exhibit defects of the glomerular basement membrane (GBM) soon after birth in a strain-dependent manner. However, the mechanisms for the onset of defects caused by deficiency remains unidentified. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00055.2020DOI Listing

Inhibition of interleukin-6 on matrix protein production by glomerular mesangial cells and the pathway involved.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1478-F1488. Epub 2020 May 11.

Department of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas.

Activation of immunological pathways and disturbances of extracellular matrix (ECM) dynamics are important contributors to the pathogenesis of chronic kidney diseases. Glomerular mesangial cells (MCs) are critical for homeostasis of glomerular ECM dynamics. Interleukin-6 (IL-6) can act as a pro/anti-inflammatory agent relative to cell types and conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00043.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311714PMC

CBX7 suppression prevents ischemia-reperfusion injury-induced endoplasmic reticulum stress through the Nrf-2/HO-1 pathway.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1531-F1538. Epub 2020 May 11.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Renal ischemia-reperfusion injury (I/R) usually occurs in renal transplantation and partial nephrectomy, which could lead to acute kidney injury. However, the effective treatment for renal I/R still remains limited. In the present study, we investigated whether inhibition of chromobox 7 (CBX7) could attenuate renal I/R injury in vivo and in vitro as well as the potential mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00088.2020DOI Listing

Depletion of macrophages slows the early progression of renal injury in obese Dahl salt-sensitive leptin receptor mutant rats.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1489-F1499. Epub 2020 May 11.

Department of Experimental Therapeutics and Pharmacology, University of Mississippi Medical Center, Jackson, Mississippi.

Recently, we reported that obese Dahl salt-sensitive (SS) leptin receptor mutant (SSmutant) rats display progressive renal injury. The present study demonstrated that the early development of renal injury in the SSmutant strain is associated with an increase in the renal infiltration of macrophages compared with lean SS rats. We also examined whether depletion of macrophages with clodronate would reduce the early progression of renal injury in the SSmutant strain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00100.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311704PMC

Developmental loss, but not pharmacological suppression, of renal carbonic anhydrase 2 results in pyelonephritis susceptibility.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1441-F1453. Epub 2020 May 11.

Division of Pediatric Nephrology, Department of Pediatrics, Indiana University, Indianapolis, Indiana.

Carbonic anhydrase II knockout () mice have depleted numbers of renal intercalated cells, which are increasingly recognized to be innate immune effectors. We compared pyelonephritis susceptibility following reciprocal renal transplantations between and wild-type mice. We examined the effect of pharmacological CA suppression using acetazolamide in an experimental murine model of urinary tract infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00583.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311712PMC

GLP-1-induced renal vasodilation in rodents depends exclusively on the known GLP-1 receptor and is lost in prehypertensive rats.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1409-F1417. Epub 2020 May 11.

Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Glucagon-like peptide-1 (GLP-1) is an incretin hormone known to stimulate postprandial insulin release. However, GLP-1 also exerts extrapancreatic effects, including renal effects. Some of these renal effects are attenuated in hypertensive rats, where renal expression of GLP-1 receptors is reduced. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00579.2019DOI Listing

Dietary fructose enhances angiotensin II-stimulated Na transport via activation of PKC-α in renal proximal tubules.

Am J Physiol Renal Physiol 2020 Jun 11;318(6):F1513-F1519. Epub 2020 May 11.

Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, Ohio.

Angiotensin II (ANG II) stimulates proximal nephron transport via activation of classical protein kinase C (PKC) isoforms. Acute fructose treatment stimulates PKC and dietary fructose enhances ANG II's ability to stimulate Na transport, but the mechanisms are unclear. We hypothesized that dietary fructose enhances ANG II's ability to stimulate renal proximal tubule Na reabsorption by augmenting PKC-α activation and increases in intracellular Ca. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00543.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311708PMC

Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells.

Am J Physiol Renal Physiol 2020 Jul 11;319(1):F41-F51. Epub 2020 May 11.

Department of Chemistry, University of Washington, Seattle, Washington.

Endothelial cells (ECs) from different human organs possess organ-specific characteristics that support specific tissue regeneration and organ development. EC specificity is identified by both intrinsic and extrinsic cues, among which the parenchyma and organ-specific microenvironment are critical contributors. These extrinsic cues are, however, largely lost during ex vivo cultures. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00069.2020DOI Listing

Sensitivity of urethral flow-evoked voiding reflexes decline with age in the rat: insights into age-related underactive bladder.

Am J Physiol Renal Physiol 2020 Jun 4;318(6):F1430-F1440. Epub 2020 May 4.

Department of Biomedical Engineering, Florida International University, Miami, Florida.

The prevalence of underactive bladder (UAB) increases with age, suggesting a link between age-related processes and lower urinary tract (LUT) symptoms; however, the underlying mechanisms of age-related UAB are poorly understood. Understanding how aging affects LUT reflexes may help in the development of new treatments by identifying mechanistic targets. In this work, we studied the relationship between age and systems-level function of the LUT and tested the hypothesis that aging is related to weakening of reflexes that control voiding. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00475.2019DOI Listing

Differences in renal BMAL1 contribution to Na homeostasis and blood pressure control in male and female mice.

Am J Physiol Renal Physiol 2020 Jun 27;318(6):F1463-F1477. Epub 2020 Apr 27.

Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, Florida.

The renal circadian clock has a major influence on the function of the kidney. Aryl hydrocarbon receptor nuclear translocator-like protein 1 [ARNTL; also known as brain and muscle ARNT-like 1 (BMAL1)] is a core clock protein and transcription factor that regulates the expression of nearly half of all genes. Using male and female kidney-specific cadherin BMAL1 knockout (KS-BMAL1 KO) mice, we examined the role of renal distal segment BMAL1 in blood pressure control and solute handling. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00014.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311713PMC

Purinergic P2X receptor, purinergic P2X receptor, and angiotensin II type 1 receptor interactions in the regulation of renal afferent arterioles in angiotensin II-dependent hypertension.

Am J Physiol Renal Physiol 2020 Jun 20;318(6):F1400-F1408. Epub 2020 Apr 20.

Department of Physiology and Hypertension and Renal Center of Excellence, Tulane University Health Science Center, New Orleans, Louisiana.

In ANG II-dependent hypertension, ANG II activates ANG II type 1 receptors (ATRs), elevating blood pressure and increasing renal afferent arteriolar resistance (AAR). The increased arterial pressure augments interstitial ATP concentrations activating purinergic P2X receptors (P2XRs) also increasing AAR. Interestingly, P2XR and P2XR inhibition reduces AAR to the normal range, raising the conundrum regarding the apparent disappearance of ATR influence. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00602.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311710PMC

Stimulation of the pelvic nerve increases bladder capacity in the PGE cat model of overactive bladder.

Am J Physiol Renal Physiol 2020 Jun 20;318(6):F1357-F1368. Epub 2020 Apr 20.

Department of Biomedical Engineering, Duke University, Durham, North Carolina.

Selective electrical stimulation of the pudendal nerve exhibits promise as a potential therapy for treating overactive bladder (OAB) across species (rats, cats, and humans). More recently, pelvic nerve (PelN) stimulation was demonstrated to improve cystometric bladder capacity in a PGE rat model of OAB. However, PelN stimulation in humans or in an animal model that is more closely related to humans has not been explored. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00068.2020DOI Listing

Genetic ablation of SLK exacerbates glomerular injury in adriamycin nephrosis in mice.

Am J Physiol Renal Physiol 2020 Jun 20;318(6):F1377-F1390. Epub 2020 Apr 20.

Departments of Medicine and Physiology, McGill University Health Centre Research Institute, McGill University, Montreal, Quebec, Canada.

Ste20-like kinase SLK is critical for embryonic development and may play an important role in wound healing, muscle homeostasis, cell migration, and tumor growth. Mice with podocyte-specific deletion of SLK show albuminuria and damage to podocytes as they age. The present study addressed the role of SLK in glomerular injury. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00028.2020DOI Listing

Intradialytic acid-base changes and organic anion production during high versus low bicarbonate hemodialysis.

Am J Physiol Renal Physiol 2020 Jun 20;318(6):F1418-F1429. Epub 2020 Apr 20.

Department of Medicine, Albert Einstein College of Medicine, Bronx, New York.

The use of high dialysate bicarbonate for hemodialysis in end-stage renal disease is associated with increased mortality, but potential physiological mediators are poorly understood. Alkalinization due to high dialysate bicarbonate may stimulate organic acid generation, which could lead to poor outcomes. Using measurements of β-hydroxybutyrate (BHB) and lactate, we quantified organic anion (OA) balance in two single-arm studies comparing high and low bicarbonate prescriptions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00036.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311706PMC

Epoxyeicosatrienoic acid metabolites inhibit Kir4.1/Kir5.1 in the distal convoluted tubule.

Am J Physiol Renal Physiol 2020 Jun 20;318(6):F1369-F1376. Epub 2020 Apr 20.

Department of Pharmacology, New York Medical College, Valhalla, New York.

Cytochrome -450 (Cyp) epoxygenase-dependent metabolites of arachidonic acid (AA) have been shown to inhibit renal Na transport, and inhibition of Cyp-epoxygenase is associated with salt-sensitive hypertension. We used the patch-clamp technique to examine whether Cyp-epoxygenase-dependent AA metabolites inhibited the basolateral 40-pS K channel (Kir4.1/Kir5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00018.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311705PMC

Integrin-β is required for the renal cystogenesis caused by ciliary defects.

Am J Physiol Renal Physiol 2020 05 20;318(5):F1306-F1312. Epub 2020 Apr 20.

Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.

Defects in the function of primary cilia are commonly associated with the development of renal cysts. On the other hand, the intact cilium appears to contribute a cystogenic signal whose effectors remain unclear. As integrin-β is required for the cystogenesis caused by the deletion of the polycystin 1 gene, we asked whether it would be similarly important in the cystogenetic process caused by other ciliary defects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00070.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294335PMC

Urine cytokines as biomarkers for diagnosing interstitial cystitis/bladder pain syndrome and mapping its clinical characteristics.

Am J Physiol Renal Physiol 2020 Jun 13;318(6):F1391-F1399. Epub 2020 Apr 13.

Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan.

The objective of the present study was to investigate the diagnostic values of urine cytokines in patients with interstitial cystitis/bladder pain syndrome (IC/BPS) and to identify their correlations with clinical characteristics. Urine samples were collected from 127 patients with IC/BPS [European Society for the Study of Interstitial Cystitis (ESSIC) types 1 and 2] and 28 controls. Commercially available multiplex immunoassays (MILLIPLEX map kits) were used to analyze 31 targeted cytokines. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00051.2020DOI Listing

Western diet induces renal artery endothelial stiffening that is dependent on the epithelial Na channel.

Am J Physiol Renal Physiol 2020 05 13;318(5):F1220-F1228. Epub 2020 Apr 13.

Research Service, Harry S. Truman Memorial Veterans' Hospital, Columbia, Missouri.

Consumption of a Western diet (WD) induces central aortic stiffening that contributes to the transmittance of pulsatile blood flow to end organs, including the kidney. Our recent work supports that endothelial epithelial Na channel (EnNaC) expression and activation enhances aortic endothelial cell stiffening through reductions in endothelial nitric oxide (NO) synthase (eNOS) and bioavailable NO that result in inflammatory and oxidant responses and perivascular fibrosis. However, the role that EnNaC activation has on endothelial responses in the renal circulation remains unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00517.2019DOI Listing

Resuscitation with PEGylated carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia.

Am J Physiol Renal Physiol 2020 05 13;318(5):F1271-F1283. Epub 2020 Apr 13.

Department of Translational Physiology, Amsterdam University Medical Center Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

PEGylated carboxyhemoglobin (PEGHbCO), which has carbon monoxide-releasing properties and plasma expansion and oxygen-carrying properties, may improve both skeletal microcirculatory flow and renal cortical microcirculatory Po (CµPo) and, subsequently, limit endotoxemia-induced acute kidney injury. Anesthetized, ventilated Wistar albino rats ( = 44) underwent endotoxemic shock. CµPo was measured in exposed kidneys using a phosphorescence-quenching method. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00513.2019DOI Listing

TCRCD4CD8 (double negative) T cells protect from cisplatin-induced renal epithelial cell apoptosis and acute kidney injury.

Am J Physiol Renal Physiol 2020 Jun 13;318(6):F1500-F1512. Epub 2020 Apr 13.

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Acute kidney injury (AKI) due to cisplatin is a significant problem that limits its use as an effective chemotherapeutic agent. T cell receptorCD4CD8 double negative (DN) T cells constitute the major T cell population in the human and mouse kidney, express programmed cell death protein (PD)-1, and protect from ischemic AKI. However, the pathophysiological roles of DN T cells in cisplatin-induced AKI is unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajprenal.00033.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311707PMC