4,991 results match your criteria American journal of physiology. Lung cellular and molecular physiology[Journal]


Indoor PM from coal combustion aggravates ovalbumin-induced asthma-like airway inflammation in BALB/c mice.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 10. Epub 2019 Apr 10.

Public health, Zunyi MEDICAL UNIVERSITY, China.

Objective: We hypothesized that indoor PM exposure from coal combustion exaggerates airway inflammation in the lung tissue of asthmatic mice induced with ovalbumin (OVA).

Methods: Forty BALB/c mice, randomly divided into 4 groups (n = 10 per group), were intratracheally instilled with normal saline alone, PM (2.5mg/ml PM alone), OVA (15μg/mL OVA alone), and PM+OVA (2. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00012.2019
Publisher Site
http://dx.doi.org/10.1152/ajplung.00012.2019DOI Listing
April 2019
5 Reads

TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 10. Epub 2019 Apr 10.

Molecular Pharmacology, University of Groningen, Netherlands.

Transforming growth factor-β (TGF-β)-induced fibroblast-to-myofibroblast differentiation contributes to remodeling in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis, but whether this impacts the ability of fibroblasts to support lung epithelial repair remains little explored. We pre-treated human lung fibroblasts (primary [phFB] or MRC5 cells) with recombinant human TGF-β to induce myofibroblast differentiation, then co-cultured them with adult mouse lung EpCAM cells to investigate their capacity to support epithelial organoid formation in vitro. While control phFB and MRC5 lung fibroblasts supported organoid formation of mouse EpCAM cells, TGF-β-pre-treatment of both phFB and MRC5 impaired organoid-supporting ability. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00400.2018DOI Listing
April 2019
1 Read

Infants with evolving bronchopulmonary dysplasia demonstrate monocyte-specific expression of IL-1 in tracheal aspirates.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 10. Epub 2019 Apr 10.

Benaroya Research Institute, United States.

Bronchopulmonary dysplasia (BPD) remains a devastating consequence of prematurity. Repeated inflammatory insults worsen lung injury, but there are no predictors for BPD-related respiratory outcomes nor targeted therapies. We sought to understand inflammatory mechanisms in evolving BPD through molecular characterization of monocytes in tracheal aspirates from infants at risk for developing BPD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00060.2019DOI Listing

Differential thermostability and response to cystic fibrosis transmembrane conductance regulator (CFTR) potentiators of human and mouse F508del-CFTR.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 10. Epub 2019 Apr 10.

Physiology, Pharmacology & Neuroscience, University of Bristol, United Kingdom.

Cross-species comparative studies have highlighted differences between human and mouse cystic fibrosis transmembrane conductance regulator (CFTR), the epithelial Cl channel defective in cystic fibrosis (CF). Here, we compare the impact of the most common CF mutation F508del on the function of human and mouse CFTR heterologously expressed in mammalian cells and their response to CFTR modulators using the iodide efflux and patch-clamp techniques. Once delivered to the plasma membrane, human F508del-CFTR exhibited a severe gating defect characterized by infrequent channel openings and was thermally unstable, deactivating within minutes at 37 °C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00034.2019DOI Listing
April 2019
1 Read

Inhaled budesonide plus surfactant reduces early preterm lung injury, but is there an effect beyond the lung?

Authors:
David Tingay

Am J Physiol Lung Cell Mol Physiol 2019 Apr 3. Epub 2019 Apr 3.

Neonatal Research, Murdoch Children's Research Institute, Australia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00138.2019DOI Listing

Development of solitary chemosensory cells in the distal lung after severe influenza injury.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 25. Epub 2019 Mar 25.

Biomedical Sciences, University of Pennsylvania, United States.

H1N1 influenza virus infection induces dramatic and permanent alveolar remodeling mediated by p63+ progenitor cell expansion in both mice and some patients with acute respiratory distress syndrome. This persistent lung epithelial dysplasia is accompanied by chronic inflammation, but the driver(s) of this pathology are unknown. This work identified de novo appearance of solitary chemosensory cells (SCCs), as defined by the tuft cell marker DCLK1, in post-influenza lungs arising in close proximity with the dysplastic epithelium, whereas uninjured lungs are devoid of SCCs. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00032.2019
Publisher Site
http://dx.doi.org/10.1152/ajplung.00032.2019DOI Listing
March 2019
6 Reads

Myristoylated rhinovirus VP4 protein activates TLR2-dependent pro-inflammatory gene expression.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 25. Epub 2019 Mar 25.

Department of Pediatrics and Communicable Diseases, Department of Molecular and Integrative Physiology, University of Michigan Medical School, United States.

Asthma exacerbations are often caused by rhinovirus (RV). We and others have shown that TLR2, a membrane surface receptor that recognizes bacterial lipopeptides and lipoteichoic acid, is required and sufficient for RV-induced pro-inflammatory responses in vitro and in vivo. We hypothesized that viral protein (VP)-4, an internal capsid protein which is myristoylated upon viral replication and externalized upon viral binding, is a ligand for TLR2. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00365.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00365.2018DOI Listing
March 2019
5 Reads

Tissue kallikrein (KLK1) regulates alveolar macrophage apoptosis early in influenza virus infection.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 25. Epub 2019 Mar 25.

CEPR, Faculté de Médecine, UMR INSERM 1100, France.

Host cell proteases are involved in influenza pathogenesis. We examined the role of tissue kallikrein 1 (KLK1) by comparing wild-type (WT) and KLK1-deficient mice infected with influenza H3N2 virus. Levels of KLK1 in lung tissue and in bronchoalveolar lavage (BAL) fluid increased substantially during infection. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00379.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00379.2018DOI Listing
March 2019
10 Reads

Caspase-1 induces smooth muscle cell growth in hypoxia-induced pulmonary hypertension.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 25. Epub 2019 Mar 25.

Department of Pulmonary Medicine, Oslo University Hospital Ullevål, Norway.

Lung diseases with hypoxia are complicated by pulmonary hypertension, leading to heart failure and death. No pharmacological treatment exists. Increased proinflammatory cytokines are found in hypoxic patients, suggesting an inflammatory pathogenesis. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00322.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00322.2018DOI Listing
March 2019
4 Reads

A non-apoptotic, endothelial barrier-protective role for caspase 3.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 25. Epub 2019 Mar 25.

Medicine, Johns Hopkins University School of Medicine, United States.

Background: Non-canonical roles for caspase 3 are emerging in the fields of cancer and developmental/differentiation biology. However little is known of non-apoptotic functions of caspase 3 in most cell types. We have recently demonstrated a disassociation between caspase 3 activation and execution of apoptosis with accompanying cytoplasmic caspase 3 sequestration and preserved endothelial barrier function. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00487.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00487.2018DOI Listing
March 2019
5 Reads

MLN4924 protects against interleukin-17A-induced pulmonary inflammation by disrupting ACT1-mediated signaling.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, China.

An excessive inflammatory response in terminal airways, alveoli, and the lung interstitium eventually leads to pulmonary hypertension and chronic obstructive pulmonary disease. Proinflammatory cytokine interleukin-17A (IL-17A) has been implicated in the pathogenesis of pulmonary inflammatory diseases. MLN4924, an inhibitor of NEDD8-activating enzyme (NAE), is associated with the treatment of various types of cancers, but its role in the IL-17A-mediated inflammatory response has not been identified. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00349.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00349.2018DOI Listing
March 2019
5 Reads

Acetyl-CoA carboxylase inhibition regulates microtubule dynamics and intracellular transport in cystic fibrosis epithelial cells.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Pediatrics, Case Western Reserve University, United States.

The use of high-dose ibuprofen as an anti-inflammatory therapy in cystic fibrosis has been shown to be an effective intervention though use is limited due to potential adverse events. Identifying the mechanism of ibuprofen efficacy would aid in the development of new therapies that avoid these adverse events. Previous findings demonstrated that ibuprofen treatment restores the regulation of microtubule dynamics in cystic fibrosis (CF) epithelial cells through a 5' adenosine monophosphate-activated protein kinase (AMPK)-dependent mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00369.2018DOI Listing
March 2019
1 Read

Activation of mTORC1/PGC1 axis promotes mitochondrial biogenesis and induces cellular senescence in the lung epithelium.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Center of Translational Medicine, Thomas Jefferson University, United States.

Cellular senescence is a biological process by which cells lose their capacity to proliferate yet remain metabolically active. Although originally considered a protective mechanism to limit the formation of cancer, it is now appreciated that cellular senescence also contributes to the development of disease, including common respiratory ailments, such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. While many factors have been linked to the development of cellular senescence, mitochondrial dysfunction has emerged as an important causative factor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00244.2018DOI Listing
March 2019
3 Reads

Senescence in the Lung: Is This Getting Old?

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00081.2019DOI Listing

Vascular TSP1-CD47 Signaling Promotes Sickle Cell-Associated Arterial Vasculopathy and Pulmonary Hypertension in Mice.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Medicine, Vascular Medicine Institute, University of Pittsburgh, United States.

Pulmonary hypertension (PH) is a leading cause of death in sickle cell disease (SCD). Hemolysis and oxidative stress are contributing factors to SCD-associated PH. We have reported that the protein thrombospondin-1 (TSP1) is elevated in the plasma of patients with SCD and by interacting with its receptor CD47 limits vasodilation of distal pulmonary arteries ex vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00302.2018DOI Listing
March 2019
2 Reads

Prophylactic treatment with MSC derived exosomes attenuates traumatic acute lung injury in rats.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Department of Anesthesiology, China-Japan Union Hospital of Jilin University, China.

Mesenchymal stem cell (MSC) is a potential strategy in the pretreatment of traumatic acute lung injury (ALI), a disease which causes inflammation and oxidative stress (OS). This study aimed to investigate whether MSCs-exosomal microRNA-124-3p (miR-124-3p) affects traumatic ALI. Initially, a traumatic ALI rat model was established using the weight-drop method. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00391.2018DOI Listing

Extracellular Vesicles in Lung Health, Disease, and Therapy: Mini-Review and Special Call for papers.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Institute of Physiology, Charité - Universitaetsmedizin Berlin, Germany.

Both physiological homeostasis and pathological disease processes in the lung typically result from complex, yet coordinated multicellular responses which are synchronized via paracrine and endocrine intercellular communication pathways. Of late, extracellular vesicles have emerged as important information shuttles that can coordinate and disseminate homeostatic and disease signals. In parallel, extracellular vesicles in biological fluids such as sputum, mucus, epithelial lining fluid, edema fluid, the pulmonary circulation, pleural fluid, and lymphatics have emerged as promising candidate biomarkers for diagnosis and prognosis in lung disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00546.2018DOI Listing
March 2019
1 Read

Lung growth after pneumonectomy: searching for the right stimuli.

Authors:
Matthias Ochs

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Institute of Anatomy, Charité - Universitaetsmedizin Berlin, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00119.2019DOI Listing

Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 20. Epub 2019 Mar 20.

Pulmonary and Critical Care Division, Northwestern University, United States.

Cardiac glycosides (CG) are used primarily for cardiac failure and have been reported to have other effects, including inhibition of viral replication. Here we set out to study mechanisms by which CG as inhibitors of the Na,K-ATPase, decrease influenza A virus (IAV) replication in the lungs. We found that CG inhibit influenza virus replication in alveolar epithelial cells by decreasing intracellular potassium which in turn inhibits protein translation, independently of viral entry, mRNA transcription, and protein degradation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00173.2018DOI Listing
March 2019
1 Read

Early Studies of Airway Submucosal Glands.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 13. Epub 2019 Mar 13.

Human Physiology, U. California - Davis, United States.

This article provides a comprehensive review of early research on the structure and function of airway submucosal glands. The literature before 1950, or so, is virtually unknown, but in addition to being of historical interest, it contains much of relevance to current research. Airway glands were first mentioned in 1602. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00068.2019DOI Listing
March 2019
1 Read

Extracellular vesicles in pulmonary hypertension: lessons from mesenchymal stromal cell-derived exosomes.

Am J Physiol Lung Cell Mol Physiol 2019 May 13;316(5):L720-L722. Epub 2019 Mar 13.

Department of Pharmacology, Center for Lung Biology, University of South Alabama , Mobile, Alabama.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00107.2019DOI Listing

The role of CD36 in endothelial albumin transcytosis.

Am J Physiol Lung Cell Mol Physiol 2019 May 6;316(5):L738-L739. Epub 2019 Mar 6.

Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, German Center for Lung Research , Giessen , Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00104.2019DOI Listing

Consumptive coagulopathy is associated with organ dysfunction during PICS.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Surgery, University of Cincinnati, Department of Surgery, United States.

Patients who survive the acute phase of sepsis can progress to persistent inflammation, immunosuppression, and catabolism syndrome (PICS). Although sepsis is characterized by early hypercoagulability and delayed hypocoagulability, coagulopathy during chronic critical illness is not fully understood. The objective of this study was to determine whether sepsis-induced PICS is associated with coagulation abnormalities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00521.2018DOI Listing
March 2019
1 Read

The lipofibroblast: more than a lipid-storage depot.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Internal Medicine, University of Iowa College of Medicine, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00109.2019DOI Listing

Age-Dependent Pulmonary Reactivity to House Dust Mite Allergen: A Model of Adult-Onset Asthma?

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Center for Health and the Environment, University of California, Davis, United States.

Asthma is a heterogeneous disease differentiated by factors like allergen sensitivity, inflammation, sex, and onset age. The mouse model is widely used for studying the early life development of allergic asthma. However, age-dependent allergen responses later in life remain relatively understudied and lack a widely accepted model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00468.2018DOI Listing
March 2019
4 Reads

Concurrent Absorption and Secretion of Airway Surface Liquids and Bicarbonate Secretion in Human Bronchioles.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Department of Pediatrics, 0830, School of Medicine, University of California-San Diego & UC Riverside, United States.

Although small airways account for the largest fraction of the total conducting airway surfaces, the epithelial fluid and electrolyte transport in small, native airway epithelia has not been well characterized. Investigations have been limited, no doubt, by the complex tissue architecture as well as by its inaccessibility, small dimensions, and lack of applicable assays, especially in human tissues. To better understand how the critically thin layer of airway surface liquid (ASL) is maintained, we applied a 'capillary'-Ussing chamber (area ≈1 mm) to measure ion transport properties of bronchioles with diameters of ~ 2 mm isolated from resected specimens of excised human lungs. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00545.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00545.2018DOI Listing
March 2019
8 Reads

Go West: translational physiology for noninvasive measurement of pulmonary gas exchange in patients with hypoxemic lung disease.

Am J Physiol Lung Cell Mol Physiol 2019 May 6;316(5):L701-L702. Epub 2019 Mar 6.

Institute of Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00515.2018DOI Listing

Expression of soluble receptor for advanced glycation end products (sRAGE) is associated with disease severity in congenital diaphragmatic hernia.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Institute for Clinical Chemistry and Clinical Pharmacology, University of Bonn.

Pulmonary hypertension (PH) and lung hypoplasia are major contributors to morbidity and mortality in newborns with congenital diaphragmatic hernia (CDH). The soluble receptor for advanced glycation end products (sRAGE) is a marker of endothelial function and might be associated with disease severity in CDH newborns. In a cohort of 30 CDH newborns and 20 healthy control newborns, sRAGE concentration was measured at birth, at 6 hours, 12 hours, 24 hours, 48 hours, and 7-10 days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00359.2018DOI Listing
March 2019
1 Read

High-power vaping injures the human lung.

Authors:
Jeffrey E Gotts

Am J Physiol Lung Cell Mol Physiol 2019 May 6;316(5):L703-L704. Epub 2019 Mar 6.

Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00099.2019DOI Listing
May 2019
1 Read

Loss of myeloid-specific protein phosphatase 2A enhances lung injury and fibrosis and results in IL-10 dependent sensitization of epithelial cell apoptosis.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Pediatrics and Communicable Disease, University of Michigan, United States.

Protein phosphatase 2A (PP2A), a ubiquitously expressed ser/thr phosphatase is an important regulator of cytokine signaling and cell function. We previously showed that myeloid-specific deletion of PP2A (LysMcrePP2A-/-) increased mortality in a murine peritoneal sepsis model. In the current study, we assessed the role of myeloid PP2A in regulation of lung injury induced by lipopolysaccharide (LPS) or bleomycin delivered intratracheally. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00299.2018DOI Listing
March 2019
3 Reads

Embryonic Mesothelium in Motion: A Systematic Study.

Authors:
Elie El Agha

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Internal Medicine, Justus-Liebig University Giessen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00082.2019DOI Listing

Surfactant plus budesonide decreases lung and systemic inflammation in mechanically ventilated preterm sheep.

Am J Physiol Lung Cell Mol Physiol 2019 Mar 6. Epub 2019 Mar 6.

Pediatrics-Neonatology, Saint Louis University, United States.

Background: Mechanical ventilation with normal tidal volumes (V) causes lung and systemic inflammation in preterm sheep. Mechanical ventilation is associated with bronchopulmonary dysplasia (BPD) in preterm infants, and the addition of budesonide to surfactant decreases BPD in clinical trials.

Hypothesis: Budesonide with surfactant will decrease the lung injury from mechanical ventilation for 24 h in preterm sheep. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00477.2018DOI Listing
March 2019
2 Reads

Regulation of IL-17A and Implications for TGF-β1 Co-modulation of Airway Smooth Muscle Remodeling in Severe Asthma.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 27. Epub 2019 Feb 27.

Pharmacology, University of Nevada, Reno.

Severe asthma develops as a result of heightened, persistent symptoms that generally coincide with pronounced neutrophilic airway inflammation. In severe asthmatics, symptoms are poorly controlled by high-dose inhaled glucocorticoids and often lead to elevated morbidity and mortality rates that underscore the necessity for novel drug target identification that overcomes limitations in disease management. Many incidences of severe asthma are mechanistically associated with T-helper cell 17 (T17)-derived cytokines and immune factors that mediate neutrophilic influx to the airways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00416.2018DOI Listing
February 2019
1 Read

Epidermal growth factor receptor (EGFR) paracrine upregulation in idiopathic pulmonary fibrosis (IPF) fibroblasts is blocked by nintedanib.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 27. Epub 2019 Feb 27.

Pulmonary Department, Meir Medical Center.

Although present in normal cells, Epidermal Growth Factor Receptor (EGFR) is overexpressed in a variety of tumors and has been associated with decreased survival. As activated fibroblasts are considered key effectors in fibrosis and since metastatic and fibrotic processes were shown to share similar signaling pathways, we investigated the contribution of EGFR signaling to Idiopathic pulmonary fibrosis (IPF) progression in lung fibroblasts derived from patients with IPF (IPF-HLF). EGFR expression and EGFR-related signaling were evaluated by Western blot and immunohistochemistry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00526.2018DOI Listing
February 2019

NADPH oxidase-mediated induction of reactive oxygen species and extracellular matrix deposition by insulin-like growth factor binding protein-5.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 27;316(4):L644-L655. Epub 2019 Feb 27.

Department of Medicine, Division of Rheumatology and Immunology, Medical University of South Carolina , Charleston, South Carolina.

Insulin-like growth factor binding protein-5 (IGFBP-5) induces production of the extracellular matrix (ECM) components collagen and fibronectin both in vitro and in vivo and is overexpressed in patients with fibrosing lung diseases, such as idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). However, the mechanism by which IGFBP-5 exerts its fibrotic effect is incompletely understood. Recent reports have shown a substantial role of reactive oxygen species (ROS) in fibrosis; thus we hypothesized that IGFBP-5 induces production of ROS to mediate the profibrotic process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00106.2018DOI Listing
April 2019
1 Read

Short-duration hyperoxia causes genotoxicity in mouse lungs: Protection by volatile anesthetic isoflurane.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 27. Epub 2019 Feb 27.

Internal Medicine, Texas Tech University Health Sciences Center, United States.

High concentrations of oxygen (hyperoxia) are routinely used during anesthesia and supplemental oxygen is also administered in connection with several other clinical conditions. Although prolonged hyperoxia is known to cause acute lung injury (ALI), whether short-duration hyperoxia causes lung toxicity remains unknown. We exposed mice to room air (RA or 21%O) or 60 % oxygen alone or in combination with 2% isoflurane for 2-hours and determined the expression of oxidative stress marker genes, DNA damage and DNA repair genes, and expression of cell cycle regulatory proteins using qPCR and Western analyses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00142.2018DOI Listing
February 2019
5 Reads

Erythropoietin inhalation enhances adult canine alveolar capillary formation following pneumonectomy.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 20. Epub 2019 Feb 20.

Internal Medicine, University of Texas Southwestern Medical Center, United States.

Paracrine erythropoietin (EPO) signaling in the lung recruits endothelial progenitor cells, promotes cell maturation and angiogenesis , and is upregulated during canine post-pneumonectomy (PNX) compensatory lung growth. To determine whether inhalational delivery of exogenous EPO augments endogenous post-PNX lung growth, adult canines underwent right PNX and received, via a permanent tracheal stoma, weekly nebulization of recombinant human EPO-containing nanoparticles or empty nanoparticles (control) for 16 weeks. Lung function was assessed under anesthesia pre- and post-PNX. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00504.2018DOI Listing
February 2019

Cellular Senescence in the Lung Across the Age Spectrum.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 20. Epub 2019 Feb 20.

Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, United States.

Cellular senescence results in cell cycle arrest with secretion of cytokines, chemokines, growth factors and remodeling proteins (senescence associated secretory phenotype; SASP) that have autocrine and paracrine effects on the tissue microenvironment. SASP can promote remodeling, inflammation, infectious susceptibility, angiogenesis and proliferation, while hindering tissue repair and regeneration. While the role of senescence and contributions of senescent cells are increasingly recognized in the context of aging and a variety of disease states, relatively less is known regarding the portfolio and influences of senescent cells in normal lung growth and aging per se, or in the induction or progression of lung diseases across the age spectrum such as bronchopulmonary dysplasia, asthma, COPD, or pulmonary fibrosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00424.2018DOI Listing
February 2019
1 Read

Monocrotaline pyrrole induces pulmonary endothelial damage through binding to and release from erythrocytes in lung during venous blood reoxygenation.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 20. Epub 2019 Feb 20.

Pathophysiology, Tongji Medical College, China.

Monocrotaline has been widely used to establish an animal model of pulmonary hypertension, most frequently in rats. An important feature of this model resides in the selectivity of monocrotaline injury towards the pulmonary vascular endothelium versus the systemic vasculature when administrated at standard dosage. The toxic metabolite of monocrotaline, monocrotaline pyrrole, is transported by erythrocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00279.2018DOI Listing
February 2019
1 Read

Delayed resolution of bleomycin-induced pulmonary fibrosis in absence of MMP13 (collagenase 3).

Am J Physiol Lung Cell Mol Physiol 2019 Feb 20. Epub 2019 Feb 20.

Universidad Nacional Autonoma de Mexico.

MMP13 deficiency in pulmonary fibrosis have described contradictory phenotypes on inflammatory and fibrotic responses after lung injury and its role during lung fibrosis resolution is still undefined. MMP13 has been considered the main collagenase in rodents, and the remodeling of fibrillar collagen is widely attributed to the action of this enzyme. In this study we aimed to explore the role of MMP13 during lung fibrosis progression and resolution. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00455.2017DOI Listing
February 2019

Exhaled breath metabolomics reveals a pathogen-specific response in a rat pneumonia model for two human pathogenic bacteria: a proof-of-concept study.

Am J Physiol Lung Cell Mol Physiol 2019 May 13;316(5):L751-L756. Epub 2019 Feb 13.

Department of Intensive Care, Amsterdam University Medical Center-Academic Medical Centre , Amsterdam , The Netherlands.

Volatile organic compounds in breath can reflect host and pathogen metabolism and might be used to diagnose pneumonia. We hypothesized that rats with () or () pneumonia can be discriminated from uninfected controls by thermal desorption-gas chromatography-mass-spectrometry (TD-GC-MS) and selected ion flow tube-mass spectrometry (SIFT-MS) of exhaled breath. Male adult rats ( = 50) received an intratracheal inoculation of ) 200 µl saline, or ) 1 × 10 colony-forming units of or 3) 1 × 10 CFU of . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00449.2018DOI Listing

Extracellular vesicles: another compartment for the second messenger, cyclic adenosine monophosphate.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 13;316(4):L691-L700. Epub 2019 Feb 13.

Department of Physiology Pharmacology, University of South Alabama , Mobile, Alabama.

The second messenger, cAMP, is highly compartmentalized to facilitate signaling specificity. Extracellular vesicles (EVs) are submicron, intact vesicles released from many cell types that can act as biomarkers or be involved in cell-to-cell communication. Although it is well recognized that EVs encapsulate functional proteins and RNAs/miRNAs, currently it is unclear whether cyclic nucleotides are encapsulated within EVs to provide an additional second messenger compartment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00282.2018DOI Listing
April 2019
4.080 Impact Factor

Prostaglandin E2 inhibits pro-fibrotic function of human pulmonary fibroblasts by disrupting Ca2+-signaling.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 13. Epub 2019 Feb 13.

Medicine, McMaster University, Canada.

We have shown that calcium (Ca) oscillations in human pulmonary fibroblasts (HPFs) contribute to pro-fibrotic effects of TGFβ and that disruption of these oscillations blunts features of pulmonary fibrosis. Prostaglandin E (PGE) exerts anti-fibrotic effects in the lung but the mechanisms for this action are not well defined. We thus sought to explore interactions between PGE and the pro-fibrotic agent transforming growth factor beta (TGFβ) in pulmonary fibroblasts (PFs) isolated from patients with or without idiopathic pulmonary fibrosis (IPF). Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00403.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00403.2018DOI Listing
February 2019
8 Reads

Galectin-3 is Expressed in Vascular Smooth Muscle Cells and Promotes Pulmonary Hypertension through changes in Proliferation, Apoptosis and Fibrosis.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 6. Epub 2019 Feb 6.

Augusta University, United States.

A defining characteristic of Pulmonary Hypertension (PH) is the extensive remodeling of pulmonary arteries (PA) that results in progressive increases in vascular resistance and stiffness and eventual failure of the right ventricle. There is no cure for PH and identification of novel molecular mechanisms that underlie increased proliferation, reduced apoptosis and excessive extracellular matrix production in pulmonary artery smooth muscle cells (PASMC) is a vital objective. Galectin-3 (Gal-3) is a chimeric lectin and potent driver of many aspects of fibrosis, but its role in regulating PASMC behavior in PH remains poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00186.2018DOI Listing
February 2019
3 Reads

Fourth generation e-cigarette vaping induces transient lung inflammation and gas exchange disturbances: results from two randomized clinical trials.

Am J Physiol Lung Cell Mol Physiol 2019 May 6;316(5):L705-L719. Epub 2019 Feb 6.

Department of Cardiology, Centre Hospitalier Universitaire Saint-Pierre, Université Libre de Bruxelles , Brussels , Belgium.

When heated by an electronic cigarette, propylene glycol and glycerol produce a nicotine-carrying-aerosol. This hygroscopic/hyperosmolar aerosol can deposit deep within the lung. Whether these deposits trigger local inflammation and disturb pulmonary gas exchanges is not known. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00492.2018DOI Listing
May 2019
2 Reads

Resolvin D1 attenuates mechanical stretch-induced pulmonary fibrosis via epithelial-mesenchymal transition (EMT).

Am J Physiol Lung Cell Mol Physiol 2019 Feb 6. Epub 2019 Feb 6.

Anesthesiology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.

Mechanical ventilation-induced pulmonary fibrosis plays an important role in the high mortality rate of acute respiratory distress syndrome (ARDS). Resolvin D1 (RvD1) displays potent proresolving activities. Epithelial-mesenchymal transition (EMT) has been proven to be an important pathological feature of lung fibrosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00415.2018DOI Listing
February 2019
2 Reads

Regulation of Ovarian cancer G protein-coupled receptor (OGR1) expression and signaling.

Am J Physiol Lung Cell Mol Physiol 2019 Feb 6. Epub 2019 Feb 6.

Medicine, Director, Center for Translational Medicine, Thomas Jefferson University, United States.

Ovarian cancer G protein-coupled receptor 1 (OGR1) is a recently deorphanized G protein-coupled receptor shown to signal in response to low extracellular pH (↓pHo) or certain benzodiazepines. The pleiotropic nature of OGR1 signaling in human airway smooth muscle (HASM) cells suggests OGR1 is a potential therapeutic target for the management of obstructive lung diseases. However, the basic pharmacological and regulatory features of OGR1 remain poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00426.2018DOI Listing
February 2019
1 Read

Mesothelial mobilization in the developing lung and heart differs in timing, quantity and pathway-dependency.

Am J Physiol Lung Cell Mol Physiol 2019 Jan 31. Epub 2019 Jan 31.

Institut für Molekularbiologie, Medizinische Hochschule Hannover, Germany.

The mesothelial lining of the lung, the visceral pleura, and of the heart, the epicardium, derive from a common multi-potent precursor tissue, the mesothelium of the embryonic thoracic cavity that also contributes to organ-specific mesenchymal cell types. Insight into mesothelial mobilization and differentiation has prevailed in the developing heart while the mesenchymal transition and fate of the visceral pleura is poorly understood. Here, we use the fact that the early mesothelium of both the lung and the heart expresses the transcription factor gene Wt1, to comparatively analyze mesothelial mobilization in the two organs by a genetic cre-loxPbased conditional approach. Read More

View Article

Download full-text PDF

Source
https://www.physiology.org/doi/10.1152/ajplung.00212.2018
Publisher Site
http://dx.doi.org/10.1152/ajplung.00212.2018DOI Listing
January 2019
5 Reads

Small-hairpin RNA and pharmacological targeting of neutral sphingomyelinase prevent diaphragm weakness in rats with heart failure and reduced ejection fraction.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 31;316(4):L679-L690. Epub 2019 Jan 31.

Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida , Gainesville, Florida.

Heart failure with reduced ejection fraction (HFREF) increases neutral sphingomyelinase (NSMase) activity and mitochondrial reactive oxygen species (ROS) emission and causes diaphragm weakness. We tested whether a systemic pharmacological NSMase inhibitor or short-hairpin RNA (shRNA) targeting NSMase isoform 3 (NSMase3) would prevent diaphragm abnormalities induced by HFREF caused by myocardial infarction. In the pharmacological intervention, we used intraperitoneal injection of GW4869 or vehicle. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00516.2018DOI Listing
April 2019
4.080 Impact Factor

Genetic inactivation of the phospholipase A activity of peroxiredoxin 6 in mice protects against LPS-induced acute lung injury.

Am J Physiol Lung Cell Mol Physiol 2019 Apr 31;316(4):L656-L668. Epub 2019 Jan 31.

Institute for Environmental Medicine, Department of Physiology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, Pennsylvania.

Peroxiredoxin 6 (Prdx6) is a multifunctional enzyme that serves important antioxidant roles by scavenging hydroperoxides and reducing peroxidized cell membranes. Prdx6 also plays a key role in cell signaling by activating the NADPH oxidase, type 2 (Nox2) through its acidic Ca-independent phospholipase A2 (aiPLA2) activity. Nox2 generation of O, in addition to signaling, can contribute to oxidative stress and inflammation such as during sepsis-induced acute lung injury (ALI). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajplung.00344.2018DOI Listing
April 2019
1 Read
4.080 Impact Factor