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    17288 results match your criteria American journal of clinical pathology[Journal]

    1 OF 346

    Improving Anatomic Pathology in Sub-Saharan Africa to Support Cancer Care.
    Am J Clin Pathol 2018 Feb 19. Epub 2018 Feb 19.
    African Strategies for Advancing Pathology, Denver, CO.
    Objectives: Cancer care requires both accurate pathologic diagnosis as well as pathologic cancer staging. We evaluated three approaches to training pathologists in sub-Saharan Africa to perform pathologic cancer staging of breast, cervix, prostate, and colorectal cancers.

    Methods: One of three training methods was used at each workshop: didactic, case-based testing (CBT), or a blended approach. Read More

    Delay to Formalin Fixation (Cold Ischemia Time) Effect on Breast Cancer Molecules.
    Am J Clin Pathol 2018 Feb 19. Epub 2018 Feb 19.
    Department of Pathology, Roswell Park Cancer Institute, Buffalo, NY.
    Objectives: The gold standard of examining breast biomarkers, including estrogen receptor (ER)/progesterone receptor (PR)/human epidermal growth factor receptor 2 (HER2)/Ki-67, is to perform these assays on formalin-fixed, paraffin-embedded tissue. However, preanalytical variables may confound these assays. One of these factors is delay to formalin fixation (DFF). Read More

    Dual Stain With SATB2 and CK20/Villin Is Useful to Distinguish Colorectal Carcinomas From Other Tumors.
    Am J Clin Pathol 2018 Feb;149(3):241-246
    Department of Pathology, The Ohio State University, Columbus.
    Objectives: Small sample size limits the number of immunostains that may be attempted in colorectal carcinoma (CRC) biopsy specimens. We investigated the utility of dual stain with special AT-rich sequence binding protein 2 (SATB2) or caudal-type homeobox 2 (CDX2) and cytokeratin 20 (CK20) or villin in identifying CRC.

    Methods: Tissue microarrays with 222 CRCs and 375 other carcinomas were built. Read More

    Effectiveness of Practices to Support Appropriate Laboratory Test Utilization: A Laboratory Medicine Best Practices Systematic Review and Meta-Analysis.
    Am J Clin Pathol 2018 Feb;149(3):197-221
    Centers for Disease Control and Prevention, Atlanta, GA.
    Objectives: To evaluate the effectiveness of practices used to support appropriate clinical laboratory test utilization.

    Methods: This review followed the Centers for Disease Control and Prevention (CDC) Laboratory Medicine Best Practices A6 cycle method. Eligible studies assessed one of the following practices for effect on outcomes relating to over- or underutilization: computerized provider order entry (CPOE), clinical decision support systems/tools (CDSS/CDST), education, feedback, test review, reflex testing, laboratory test utilization (LTU) teams, and any combination of these practices. Read More

    What's to Be Done About Laboratory Quality? Process Indicators, Laboratory Stewardship, the Outcomes Problem, Risk Assessment, and Economic Value: Responding to Contemporary Global Challenges.
    Am J Clin Pathol 2018 Feb;149(3):186-196
    Education and Management Division, Melbourne Pathology, Melbourne, Australia.
    Objectives: For 50 years, structure, process, and outcomes measures have assessed health care quality. For clinical laboratories, structural quality has generally been assessed by inspection. For assessing process, quality indicators (QIs), statistical monitors of steps in the clinical laboratory total testing, have proliferated across the globe. Read More

    Influence of Prior Knowledge of Human Papillomavirus Status on the Performance of Cytology Screening.
    Am J Clin Pathol 2018 Feb 17. Epub 2018 Feb 17.
    Instituto Nacional de Ciência e Tecnologia das Doenças do Papilomavírus Humano, São Paulo, Brazil.
    Objectives: This study aimed to evaluate the influence of prior knowledge of human papillomavirus (HPV) status in cervical cytopathology readings.

    Methods: Participants comprised 2,376 women who underwent parallel cytology and HPV-DNA testing. Smears were read twice by the same team, first with previous knowledge of HPV-DNA status. Read More

    Histoplasma Urinary Antigen Testing Obviates the Need for Coincident Serum Antigen Testing.
    Am J Clin Pathol 2018 Feb 15. Epub 2018 Feb 15.
    Department of Pathology and Laboratory Medicine, Cleveland Veterans Affairs Medical Center, Cleveland, OH.
    Objectives: Serum and urine antigen (SAg, UAg) detection are common tests for Histoplasma capsulatum. UAg detection is more widely used and reportedly has a higher sensitivity. We investigated whether SAg detection contributes meaningfully to the initial evaluation of patients with suspected histoplasmosis. Read More

    Quality Improvement After Multiple Fatal Transfusion-Transmitted Bacterial Infections.
    Am J Clin Pathol 2018 Feb 15. Epub 2018 Feb 15.
    Department of Pathology, University of Utah School of Medicine, Salt Lake City.
    Objectives: Transfusion-transmitted bacterial infection (TTBI) from platelet components is likely underrecognized and can be fatal. Twenty-four-hour prospective culture was felt to be insufficiently preventive after multiple TTBIs occurred and strategies to improve safety were sought.

    Methods: Two fatal and one severe TTBIs occurred from a split-apheresis platelet donation contaminated with Klebsiella pneumoniae. Read More

    Daily Biopsy Diagnosis in Surgical Pathology: Concordance Between Light Microscopy and Whole-Slide Imaging in Real-Life Conditions.
    Am J Clin Pathol 2018 Feb 14. Epub 2018 Feb 14.
    Département de Biologie et Pathologie Médicales, Service de Pathologie Morphologique, Villejuif, France.
    Objectives: The current challenge for the various digital whole-slide imaging (WSI) systems is to be definitively validated for diagnostic purposes. We designed a concordance study between glass slide and digital slide diagnosis in real-life conditions, coupled with an ergonomic study.

    Methods: Three senior pathologists evaluated, first in glass slides and then in digital slides, 119 biopsy cases, including 749 slides, with 332 H&E saffron stains and 417 additional techniques, mainly immunohistochemistry. Read More

    Giant Cell Tumor of Bone in Patients 55 Years and Older: A Study of 34 Patients.
    Am J Clin Pathol 2018 Feb;149(3):222-233
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
    Objectives: Most giant cell tumors of bone (GCTs) occur in patients aged 20 to 40 years. We analyzed features of GCT in patients 55 years or older.

    Methods: GCTs were examined for fibrosis, matrix, cystic change, histiocytes, mitoses, and necrosis. Read More

    Lyme Disease Testing in a High-Incidence State: Clinician Knowledge and Patterns.
    Am J Clin Pathol 2018 Feb;149(3):234-240
    Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado.
    Objectives: Lyme disease (LD) incidence is increasing, but data suggest some clinicians are not fully aware of recommended procedures for ordering and interpreting diagnostic tests. The study objective was to assess clinicians' knowledge and practices regarding LD testing in a high-incidence region.

    Methods: We distributed surveys to 1,142 clinicians in the University of Vermont Medical Center region, of which 144 were completed (12. Read More

    Systematic Review of Non-ASCP Choosing Wisely Recommendations Relevant to Pathology and Laboratory Medicine.
    Am J Clin Pathol 2018 Feb;149(3):267-274
    Quest DiagnosticsLos Angeles, CA.
    Objectives: To determine non-American Society for Clinical Pathology pathology- and laboratory-related Choosing Wisely recommendations that drive effective test utilization in the laboratory.

    Methods: Data were collected via a two-part web-based survey distributed to a broad sample of pathologists and laboratory professionals from a variety of institutions.

    Results: Pathologists' most relevant recommendation: "Do not transfuse more units of blood than absolutely necessary"; highest priority: "Do not transfuse more than the minimum number of RBC units necessary to relieve symptoms of anemia or to return a patient to a safe hemoglobin range (7-8 g/dL in stable, noncardiac inpatients). Read More

    Repeating Critical Hematology and Coagulation Values Wastes Resources, Lengthens Turnaround Time, and Delays Clinical Action.
    Am J Clin Pathol 2018 Feb;149(3):247-252
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY.
    Objectives: To determine the need and impact of repeating critical values in hematology and coagulation.

    Methods: We prospectively evaluated the need for repeating critical values. The cost of this practice was estimated using a workflow analysis. Read More

    Expression of Programmed Death Ligand 1 (PD-L1) in Posttreatment Primary Inflammatory Breast Cancers and Clinical Implications.
    Am J Clin Pathol 2018 Feb;149(3):253-261
    Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston.
    Objectives: Inflammatory breast carcinoma (IBC) is rare but is the most lethal type of breast cancer. Programmed death ligand 1 (PD-L1) expression in IBCs has been understudied.

    Methods: In this study, tissue microarrays of 68 IBCs were immunostained with a PD-L1 antibody using an antibody clone (28-8) and detection system approved by the US Food and Drug Administration for selecting patients with non-small cell lung cancer and melanoma for anti-PD-L1 therapy. Read More

    Serum Prostate-Specific Antigen (PSA) Concentration, PSA Mass, and Obesity: A Mathematical Analysis.
    Am J Clin Pathol 2018 Feb;149(3):262-266
    VA and Duke University Medical Centers, Durham, NC.
    Objectives: To provide a mathematical background for understanding the phenomenon of analyte hemodilution using a kinetic analysis.

    Methods: The first assumption for this analysis is that change in concentration of any analyte, such as prostate-specific antigen (PSA), is due to the flux of the analyte from an organ into the blood minus its flux from the blood. What results is a relatively simple differential equation that emphasizes the importance of plasma volume, organ mass, and two rate constants. Read More

    Crystal-Storing Histiocytosis in Bone Marrow: A Clinicopathologic Study of Eight Cases and Review of the Literature.
    Am J Clin Pathol 2018 Jan;149(2):148-163
    Department of Laboratory Medicine and Pathology, Division of Hematopathology, Mayo Clinic, Rochester, MN.
    Objectives: We report the clinicopathologic characteristics of eight cases of crystal-storing histiocytosis (CSH) with bone marrow (BM) involvement (BM-CSH) and review CSH cases published in the English literature.

    Methods: We queried our pathology database for BM cases with CSH mentioned in the final diagnosis/comments from June 2011 to August 2016.

    Results: Eight cases of BM-CSH were identified. Read More

    Integrated Analysis of HER2 Copy Number by Cytogenomic Microarray in Breast Cancers With Nonclassical In Situ Hybridization Results.
    Am J Clin Pathol 2018 Jan;149(2):135-147
    Division of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
    Objectives: To develop and test an integrated approach to human epidermal growth factor receptor 2 (HER2) copy number analysis in breast cancer using in situ hybridization (ISH) and cytogenomic microarray (CMA).

    Methods: CMA was performed on four clinical breast cancer samples with nonclassical patterns of HER2 ISH results. Integrated analysis was performed by correlating the data from pathology review, ISH, and CMA. Read More

    Newly Emerging Drugs of Abuse and Their Detection Methods: An ACLPS Critical Review.
    Am J Clin Pathol 2018 Jan;149(2):105-116
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA.
    Objectives: Illicit drug abuse has reached an epidemic level in the United States. Drug overdose has become the leading cause of injury-related deaths since 2008 due to the recent surge of opioid overdose by heroin, controlled prescription drugs, and nonmethadone synthetic opioids. Synthetic designer drugs such as synthetic cathinones ("bath salts") and synthetic cannabinoids ("Spice" and "K2") continue to emerge and attract recreational users. Read More

    Abdominopelvic and Retroperitoneal Low-Grade Fibromyxoid Sarcoma: A Clinicopathologic Study of 13 Cases.
    Am J Clin Pathol 2018 Jan;149(2):128-134
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
    Objectives: Low-grade fibromyxoid sarcoma (LGFMS) is a rare tumor often arising in the lower extremities. Only rare examples in the abdominal cavity, pelvis, and retroperitoneum have been reported.

    Methods: cases of abdominopelvic and retroperitoneal LGFMS were retrieved. Read More

    Precision of CAPILLARYS 2 for the Detection of Hemoglobin Variants Based on Their Migration Positions.
    Am J Clin Pathol 2018 Jan;149(2):172-180
    Genetic Biochemistry Department, Henri Mondor Hospital, Creteil, France.
    Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument.

    Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Read More

    Practical Diagnostic Approach to the Presence of Hyphae in Neuropathology Specimens With Three Illustrative Cases.
    Am J Clin Pathol 2018 Jan;149(2):98-104
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA.
    Objectives: Early and accurate diagnosis remains crucial in the therapeutic management of invasive central nervous system fungal infections. Different molds have intrinsic resistance to antifungal agents; thus, morphologic differentiation is helpful to clinicians.

    Methods: Using three examples, we present a guide on how to approach neuropathology specimens where hyphae are identified on initial histologic examination. Read More

    Comparison of a Medical-Grade Monitor vs Commercial Off-the-Shelf Display for Mitotic Figure Enumeration and Small Object (Helicobacter pylori) Detection.
    Am J Clin Pathol 2018 Jan;149(2):181-185
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
    Objectives: To examine the performance of a commercial off-the-shelf (COTS) monitor vs a medical-grade (MG) monitor for small object enumeration in standardized digital pathology images.

    Methods: Pathologists reviewed 35 melanoma or 35 gastric biopsy images using the MG and COTS displays, with a 2-week washout period. Mitotic figure or Helicobacter pylori burden enumerations were compared with reference values reported by an expert subspecialist pathologist using a light microscope. Read More

    Overexpression of Forkhead Box O3a and Its Association With Aggressive Phenotypes and Poor Prognosis in Human Hepatocellular Carcinoma.
    Am J Clin Pathol 2018 Jan;149(2):117-127
    Departments of Pathology, Hanyang University College of Medicine, Seoul, Korea.
    Objectives: Recent research has demonstrated that forkhead box O3a (FoxO3a) may function as an oncogenic transcription factor. We sought to validate the clinicopathologic significance of FoxO3a expression in hepatocellular carcinoma (HCC).

    Methods: Western blotting and immunohistochemistry were used to determine FoxO3a expression. Read More

    T-Cell Large Granular Lymphocytic Leukemia and Coexisting B-Cell Lymphomas: A Study From the Bone Marrow Pathology Group.
    Am J Clin Pathol 2018 Jan;149(2):164-171
    Cleveland Clinic, Cleveland, OH.
    Objective: T-cell large granular lymphocytic (T-LGL) leukemia is associated with B-cell lymphomas (BCLs), especially small BCLs. We aimed to explore and expand upon its association with BCLs.

    Methods: We retrospectively studied clinicopathologic features of T-LGL leukemia patients with coexisting BCL from January 2001 to December 2016. Read More

    Assessment of the Measurement Error in Cyclosporine Levels Drawn Between Peripheral and Central Sources.
    Am J Clin Pathol 2017 Dec;149(1):76-81
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada.
    Objectives: Cyclosporine is often monitored by drug levels drawn through central venous catheters (CVCs), which may be falsely elevated due to reversible drug adsorption onto the catheter. Therefore, we assessed the correlation between cyclosporine levels drawn peripherally and through CVCs.

    Methods: Bone marrow transplantation patients had a weekly collection of both peripheral and CVC draws from dual-lumen catheters simultaneously to assess cyclosporine levels after research ethics approval. Read More

    Overexpression of SOX11 and TFE3 in Solid-Pseudopapillary Neoplasms of the Pancreas.
    Am J Clin Pathol 2017 Dec;149(1):67-75
    Department of Pathology, Duke University Medical Center, Durham, NC.
    Objectives: To characterize the expression of SOX11 and TFE3 proteins in solid-pseudopapillary neoplasms (SPNs) and their histologic mimickers.

    Methods: Immunohistochemistry for SOX11, TFE3, and β-catenin was performed on 31 cases of surgically resected SPNs. Neuroendocrine tumors, acinar cell carcinomas, and pancreatoblastomas served as controls. Read More

    The 11q-Gain/Loss Aberration Occurs Recurrently in MYC-Negative Burkitt-like Lymphoma With 11q Aberration, as Well as MYC-Positive Burkitt Lymphoma and MYC-Positive High-Grade B-Cell Lymphoma, NOS.
    Am J Clin Pathol 2017 Dec;149(1):17-28
    Cytogenetic Laboratory, Maria Sklodowska-Curie Institute Oncology Center, Warsaw, Poland.
    Objectives: The latest revision of lymphoma's World Health Organization classification describes the new provisional entity "Burkitt-like lymphoma with 11q aberration" (BLL, 11q) as lacking MYC rearrangement, but harboring the specific11q-gain/loss aberration. We report genetic characteristics of 11 lymphoma cases with this aberration.

    Methods: Classical cytogenetics, fluorescence in situ hybridization (FISH), and single nucleotide polymorphism/array comparative genomic hybridization. Read More

    Algorithm to Manage Inconclusive RBC Antibodies by Reflex Manual Testing.
    Am J Clin Pathol 2017 Dec;149(1):29-34
    Department of Pathology and Laboratory Medicine, University of Maryland School of Medicine, Baltimore.
    Objectives: Inconclusive RBC antibody identification (ABID) may delay RBC crossmatch. An increased number of inconclusive ABID was observed, and an algorithm was developed to improve ABID efficiency.

    Methods: RBC antibody screen (AS) and ABID were initially performed using solid-phase RBC adherence assay (SPRCA) and manual tube method. Read More

    Impact of the Reclassification of "Noninvasive Encapsulated Follicular Variant of Papillary Thyroid Carcinoma" to "Noninvasive Follicular Thyroid Neoplasm With Papillary-Like Nuclear Features" on the Bethesda System for Reporting Thyroid Cytopathology: A Large Academic Institution's Experience.
    Am J Clin Pathol 2017 Dec;149(1):50-54
    Department of Pathology, New York University School of Medicine, New York, NY.
    Objectives: Noninvasive encapsulated follicular variant of papillary thyroid carcinoma (NEFVPTC) was recently reclassified as noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP). Excluding "carcinoma" from the new terminology shifted NIFTP out of the malignant category and altered Bethesda System for Reporting Thyroid Cytopathology (BSRTC) rates of malignancy (ROMs) on thyroid fine-needle aspiration (FNA). Because of potential effects on management guidelines, we examined our ROM data. Read More

    Cost Analysis of Operating an Anatomic Pathology Laboratory in a Middle-Income Country.
    Am J Clin Pathol 2017 Dec;149(1):1-7
    School of Public Health and Health Systems, University of Waterloo, Waterloo, Canada.
    Objective: To examine the cost of operating an anatomic pathology laboratory in a teaching hospital in Malaysia. Once the cost is determined, compare it with the costs of operating other laboratories in the same hospital, and operating anatomic pathology laboratories in other countries.

    Methods: Cost and workload data were obtained from hospital records for 2015. Read More

    Hemolysis in In-Date RBC Concentrates.
    Am J Clin Pathol 2017 Dec;149(1):35-41
    Roger Williams Hospital, Providence, RI.
    Objectives: Hemolysis is one of the most prominent changes that occur during the liquid storage of RBCs in additive solution (AS), but most studies have measured hemolysis only on day 42.

    Methods: Prestorage leukoreduced RBCs in AS-1 and AS-3 were studied, one group on day 42 and a second group between day 0 and day 40. Each product was sampled for direct measurement of supernatant hemoglobin and hematocrit. Read More

    Myeloid Neoplasms Following Solid Organ Transplantation: Clinicopathologic Studies of 23 Cases.
    Am J Clin Pathol 2017 Dec;149(1):55-66
    Department of Pathology, Duke University Medical Center, Durham, NC.
    Objectives: Myeloid neoplasms (MNs) after solid organ transplant are rare, and their clinicopathologic features have not been well characterized.

    Methods: We retrospectively analyzed 23 such cases.

    Results: The ages ranged from 2 to 76 years, with a median of 59 years at the diagnosis. Read More

    Epstein-Barr Virus-Positive Extranodal Marginal Zone Lymphoma of Bronchial-Associated Lymphoid Tissue in the Posttransplant Setting: An Immunodeficiency-Related (Posttransplant) Lymphoproliferative Disorder?
    Am J Clin Pathol 2017 Dec;149(1):42-49
    Division of Hematopathology, Department of Pathology and Laboratory Medicine.
    Objectives: Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of hematolymphoid proliferations arising in the context of chronic immunosuppression. The common and indolent B-cell lymphomas, including extranodal marginal zone lymphomas (ENMZLs) of mucosa-associated lymphoid tissue (MALT), are excluded from the category of PTLD in the current World Health Organization classification.

    Methods: We report a case of Epstein-Barr virus (EBV)-positive bronchial-associated lymphoid tissue (BALT) lymphoma involving the lungs of a transplant patient. Read More

    Prophylactic Preprocedure Platelet Transfusion Is Associated With Increased Risk of Thrombosis and Mortality.
    Am J Clin Pathol 2017 Dec;149(1):87-94
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY.
    Objectives: We evaluated thrombosis and mortality rates of hospitalized patients receiving prophylactic platelet transfusion prior to an invasive procedure.

    Methods: Patient age and underlying medical condition(s), preprocedure and postprocedure platelet counts, type of procedure, number of platelet products transfused, and any complications were recorded on every prophylactic platelet given prior to an invasive procedure.

    Results: A total of 376 prophylactic transfusion recipients were identified. Read More

    Immunolocalization of CD163+ Tumor-Associated Macrophages and Symmetric Proliferation of Ki-67 as Biomarkers to Differentiate New Different Grades of Laryngeal Dysplasia.
    Am J Clin Pathol 2017 Dec;149(1):8-16
    Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, China.
    Objectives: To evaluate CD163+ tumor-associated macrophages (TAMs), Ki-67, and cyclin D1 to differentiate laryngeal dysplasia in the 2017 World Health Organization classification.

    Methods: Immunohistochemistry for CD163, Ki-67, and cyclin D1 was performed using paraffin-embedded specimens. CD163+ TAMs infiltrating the epithelium were estimated. Read More

    Low pO2 Contributes to Potential Error in Oxygen Saturation Calculations Using a Point-of-Care Assay.
    Am J Clin Pathol 2017 Dec;149(1):82-86
    Department of Laboratory Medicine and Pathology.
    Objectives: The present study addressed the accuracy of calculated oxygen saturation (sO2) using point-of-care (POC) testing compared with measured values on a blood gas analyzer.

    Methods: In total, 3,323 sO2 values were measured in 1,180 patients using a CO-oximeter (ABL 800 Flex; Radiometer, Copenhagen, Denmark). Measured parameters were then used to calculate an expected sO2 for the POC method (Abbott i-STAT; Abbott POC, Princeton, NJ). Read More

    Impact of Laboratory Charge Display Within the Electronic Health Record Across an Entire Academic Medical Center: Results of a Randomized Controlled Trial.
    Am J Clin Pathol 2017 Nov;148(6):513-522
    Department of Biomedical Informatics, University of Utah, Salt Lake City.
    Objectives: To determine the impact of systemwide charge display on laboratory utilization.

    Methods: This was a randomized controlled trial with a baseline period and an intervention period. Tests were randomized to a control arm or an active arm. Read More

    Preferential Usage of Specific Immunoglobulin Heavy Chain Variable Region Genes With Unmutated Profile and Advanced Stage at Presentation Are Common Features in Patients With Chronic Lymphocytic Leukemia From Senegal.
    Am J Clin Pathol 2017 Nov;148(6):545-554
    Department of Experimental, Diagnostic, and Experimental Medicine, Bologna University School of Medicine, Bologna, Italy.
    Objectives: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in Western populations, being rarer in Asian and African people. It has been suggested that patients with CLL from Africa might have a more aggressive disease compared with white patients. In this study, we aimed to identify genetic factors that may account for this difference. Read More

    Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease.
    Am J Clin Pathol 2017 Nov;148(6):502-512
    Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing, China.
    Objectives: Investigate subtle fibrosis similarities and differences in adult and pediatric nonalcoholic fatty liver disease (NAFLD) using second harmonic generation (SHG).

    Methods: SHG/two-photon excitation fluorescence imaging quantified 100 collagen parameters and determined qFibrosis values by using the nonalcoholic steatohepatitis (NASH) Clinical Research Network (CRN) scoring system in 62 adult and 36 pediatric NAFLD liver specimens.

    Results: Six distinct parameters identified differences among the NASH CRN stages with high accuracy (area under the curve, 0835-0. Read More

    Impact of the Pathologist on Prostate Biopsy Diagnosis and Immunohistochemical Stain Usage Within a Single Institution.
    Am J Clin Pathol 2017 Nov;148(6):494-501
    Department of Pathology, The Ohio State University Medical Center, Columbus.
    Objectives: To determine whether pathologists in a tertiary care institution vary in diagnosis and immunohistochemical stain usage in prostate biopsy specimens.

    Methods: Men who underwent prostate needle biopsies between 2008 and 2013 were included.

    Results: In total, 1,777 prostate biopsy specimens diagnosed by nine pathologists showed variation in diagnostic reporting (atypical small acinar proliferation, 2. Read More

    Philadelphia Chromosome-like Mixed-Phenotype Acute Leukemia Demonstrating P2RY8-CRLF2 Fusion and JAK1 Mutation.
    Am J Clin Pathol 2017 Nov;148(6):523-528
    Departments of Pathology.
    Objectives: Philadelphia chromosome-like (Ph-like) genetic alterations define a subset of B lymphoblastic leukemia/lymphoma (B-ALL), which represents a separate provisional entity in the World Health Organization 2016 updated classification. However, these alterations have not been described outside the context of B-ALL.

    Methods: Cytogenomic array and molecular analysis identified a Ph-like signature in a mixed-phenotype acute leukemia (MPAL), B/myeloid, confirmed using conventional immunophenotypic and cytochemical analysis. Read More

    The Apoptotic Crypt Abscess: An Underappreciated Histologic Finding in Gastrointestinal Pathology.
    Am J Clin Pathol 2017 Nov;148(6):538-544
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
    Objectives: To differentiate apoptotic crypt abscesses (ACAs) from neutrophilic crypt abscesses (NCAs).

    Methods: Cases with crypt abscesses were classified as containing ACAs, NCAs, or mixed crypt abscesses (MCAs) by H&E staining. Sections were stained with cleaved caspase 3 and myeloperoxidase and recategorized. Read More

    Evaluation of Opportunities to Improve Hematopathology Diagnosis for Vietnam Pathologists.
    Am J Clin Pathol 2017 Nov;148(6):529-537
    Center for Global Health, American Society for Clinical Pathology.
    Objectives: We evaluate the need for, feasibility of, and impediments to improving hematopathology diagnoses for cancer hospitals in Vietnam.

    Methods: Two hematopathologists from the United States visited three major cancer treatment hospitals in Vietnam to workshop a sampling of difficult hematopathology cases. With Vietnamese pathologists, they toured histopathology, immunohistochemistry, and ancillary laboratory facilities. Read More

    KLHL6 Is Preferentially Expressed in Germinal Center-Derived B-Cell Lymphomas.
    Am J Clin Pathol 2017 Nov;148(6):465-476
    Department of Pathology.
    Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells.

    Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Read More

    CD30 Expression in Monomorphic Posttransplant Lymphoproliferative Disorder, Diffuse Large B-Cell Lymphoma Correlates With Greater Regulatory T-Cell Infiltration.
    Am J Clin Pathol 2017 Nov;148(6):485-493
    Department of Pathology, Medical College of Wisconsin, Milwaukee.
    Objectives: CD30 is a protein thought to promote cell proliferation/survival and downregulate the immune response. Twenty percent to 40% of de novo diffuse large B-cell lymphomas (DLBCLs) express CD30, and some patients have been treated with the anti-CD30 agent brentuximab. In the solid organ transplant setting, allograft regulatory T cells (Tregs) have been shown to be modulated via CD30 signaling. Read More

    Gastric Carcinomas With Lymphoid Stroma: Categorization and Comparison With Solid-Type Colonic Carcinomas.
    Am J Clin Pathol 2017 Nov;148(6):477-484
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.
    Objectives: To determine whether histologic features could help identify gastric carcinomas with lymphoid stroma associated with microsatellite instability (MSI) (ie, "medullary carcinomas"), Epstein-Barr virus (EBV) infection (termed lymphoepithelioma-like carcinomas in other organ systems), or neither.

    Methods: We identified 17 solid-type gastric carcinomas with lymphoid stroma, assessed EBV and MSI status, and compared features across groups. We also compared them with 51 solid-type colorectal adenocarcinomas. Read More

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