17,718 results match your criteria American journal of clinical pathology[Journal]


Cytoplasmic Expression of CD3ε Heterodimers by Flow Cytometry Rapidly Distinguishes Between Mature T-Cell and Natural Killer-Cell Neoplasms.

Am J Clin Pathol 2020 Jun 26. Epub 2020 Jun 26.

Division of Hematopathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.

Objectives: Distinguishing between T-cell and natural killer (NK)-cell neoplasms could be difficult given their overlapping immunophenotype. In this study, we investigated whether a flow cytometry assay with cytoplasmic staining for CD3 could be used for this purpose.

Methods: Flow cytometry immunophenotyping was performed on 19 surface CD3 (sCD3)-negative mature T-cell neoplasms, 10 sCD3-positive mature T-cell neoplasms, 13 mature NK-cell neoplasms, and 19 normal controls. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa086DOI Listing

Endoscopic Ultrasound-Guided Fine Needle Aspiration Cytologic Evaluation of Intraductal Papillary Mucinous Neoplasm and Mucinous Cystic Neoplasms of Pancreas.

Am J Clin Pathol 2020 Jun 26. Epub 2020 Jun 26.

Department of Pathology, Yale School of Medicine, New Haven, CT.

Objectives: To evaluate the role of endoscopic ultrasound-guided fine needle aspiration cytology in identifying mucinous cystic lesions (MCLs) in histologically proven cases of intraductal papillary mucinous neoplasm (IPMN) or mucinous cystic neoplasm (MCN) and risk of malignancy associated with each cytologic category based on the Papanicolaou Society of Cytopathology (PSC) guidelines.

Methods: All resected cases with histologic diagnosis of IPMN or MCN at our institution from January 1, 2004, to August 31, 2019, with associated cytology were included. Available cytology slides of nondiagnostic (ND), negative/benign (BN), and atypical cytology (AC) cases were reviewed and reclassified based on the PSC guidelines. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa079DOI Listing

Scoring of Programmed Death-Ligand 1 Immunohistochemistry on Cytology Cell Block Specimens in Non-Small Cell Lung Carcinoma.

Am J Clin Pathol 2020 Jun 26. Epub 2020 Jun 26.

Department of Pathology, Department of Population Health, NYU Langone Health, New York, NY.

Objectives: Recent investigations have shown strong correlations between cytology and surgical non-small cell lung carcinoma (NSCLC) specimens in programmed death-ligand 1 (PD-L1) immunohistochemical (IHC) evaluations. Our study aims to evaluate the reproducibility of PD-L1 IHC scoring in NSCLC cytology cell blocks (CBs) and to assess the impact of CB cellularity, method of sample collection, and observer subspecialty on scoring agreement.

Methods: PD-L1 IHC was performed on 54 NSCLC cytology CBs and was scored independently by seven cytopathologists (three of seven with expertise in pulmonary pathology). Read More

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http://dx.doi.org/10.1093/ajcp/aqaa073DOI Listing

Transfusion Service Response to the COVID-19 Pandemic.

Am J Clin Pathol 2020 Jun 25. Epub 2020 Jun 25.

Department of Pathology, Virginia Commonwealth University School of Medicine, Richmond.

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http://dx.doi.org/10.1093/ajcp/aqaa111DOI Listing

Review of Current Advances in Serologic Testing for COVID-19.

Am J Clin Pathol 2020 Jun 25. Epub 2020 Jun 25.

Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine and Jackson Health System, Miami, FL.

Objectives: To examine and summarize the current literature on serologic methods for the detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Methods: A literature review was performed using searches in databases including PubMed, medRxiv, and bioRxiv. Thirty-two peer-reviewed papers and 23 preprints were examined. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa112DOI Listing

Antibody Testing for COVID-19.

Am J Clin Pathol 2020 Jun 24. Epub 2020 Jun 24.

Case Western Reserve University School of Dental Medicine Cleveland, OH.

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http://dx.doi.org/10.1093/ajcp/aqaa110DOI Listing

Interobserver Variability in Ductal Carcinoma In Situ of the Breast.

Am J Clin Pathol 2020 Jun 22. Epub 2020 Jun 22.

Department of Pathology, Brussels, Belgium.

Objectives: Since most patients with ductal carcinoma in situ (DCIS) of the breast are treated upon diagnosis, evidence on its natural progression to invasive carcinoma is limited. It is estimated that around half of the screen-detected DCIS lesions would have remained indolent if they had never been detected. Many patients with DCIS are therefore probably overtreated. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa077DOI Listing

Histologic Findings of Uterine Niches.

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

Pathology Department, France.

Objectives: The disruption or defect of the myometrium in the uterine scar of a cesarean section (CS) has been known by various names, such as uterine niche, isthmocele, deficient uterine scar, scar pouch, or diverticulum. Symptomatology, risk factors for niche development, and available treatment modalities have been recently studied. However, the histologic features of this disease remain unknown. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa080DOI Listing

CALIPER Hematology Reference Standards (I).

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Canada.

Objectives: Accurate hematologic test interpretation based on normative reference standards is critical to ensure appropriate clinical decision making. However, healthy pediatric reference data for most hematology parameters are lacking. To address this gap, this study establishes age- and sex-specific hematologic reference standards in the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) cohort of healthy children and adolescents. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa059DOI Listing

Juvenile Granulosa Cell Tumors of the Ovary.

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.

Objective: To explore the clinical and pathologic features of ovarian juvenile granulosa cell tumors (JGCTs).

Methods: Clinical data, histopathologic observations, immunohistochemical results, FOXL2 mutation status, and follow-up information of 7 JGCT cases were studied.

Results: The patients most commonly presented with abdominal distension and pain (5 cases), followed by precocious puberty (1 case) and a pelvic mass (1 case). Read More

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http://dx.doi.org/10.1093/ajcp/aqaa081DOI Listing

Steatohepatitis-Like Changes in Hepatocellular Adenoma.

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

Department of Pathology, Seattle.

Objectives: Our aim was to investigate the frequency of steatohepatitic morphology in hepatocellular adenoma (HCA) and correlate with its clinical parameters and risk factors underlying nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH).

Methods: We examined a series of 41 liver resection specimens diagnosed with HCA for steatohepatitic changes. Background nonneoplastic liver was also evaluated. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa075DOI Listing

Voxelotor Treatment Interferes With Quantitative and Qualitative Hemoglobin Variant Analysis in Multiple Sickle Cell Disease Genotypes.

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: Voxelotor was recently approved for use in the United States as a treatment for sickle cell disease (SCD) and has been shown to interfere with the quantitation of hemoglobin (Hb) S percentage. This study aimed to determine the effect of voxelotor on the quantitation of hemoglobin variant levels in patients with multiple SCD genotypes.

Methods: In vitro experiments were performed to assess the impact of voxelotor treatment on hemoglobin variant testing. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa067DOI Listing

Data-Driven Development of an Institutional "Gross-Only" Policy for the Examination of Select Surgical Pathology Specimens.

Am J Clin Pathol 2020 Jun 20. Epub 2020 Jun 20.

Medical College of Wisconsin, Department of Pathology, Milwaukee.

Objectives: To determine diagnostic, workflow, and economic implications of instituting a gross-only policy at our institution.

Methods: Retrospective (2017) key word searches were performed to identify "gross-only" cases for which microscopic evaluation could potentially be omitted, but was performed, and those who underwent gross evaluation per surgeon request. Cases were evaluated for type(s), part(s), block volume, turnaround time, demographics, and diagnosis. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa065DOI Listing

The Importance of Tumor Length in Needle Biopsies of the Prostate.

Authors:
Robin T Vollmer

Am J Clin Pathol 2020 Jun 18. Epub 2020 Jun 18.

Duke University Medical Centers, Durham, NC.

Objectives: To form a composite predictor variable that combines the effects of tumor length, serum prostate-specific antigen (PSA), and International Society of Urologic Pathologists (ISUP) grade on the observation of adverse prostatectomy pathology.

Methods: Logistic regression analysis was used to demonstrate how tumor length, serum PSA, and ISUP grade related to adverse prostatectomy results and to derive weighting factors for a composite variable, cx.

Results: The composite variable, cx, relates closely to adverse prostatectomy results as well as to observed PSA failure. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa068DOI Listing

Engrailed Homeobox 1 and Cytokeratin 19 Are Independent Diagnostic Markers of Eccrine Porocarcinoma and Distinguish It From Squamous Cell Carcinoma.

Am J Clin Pathol 2020 Jun 18. Epub 2020 Jun 18.

Department of Pathology, Yokohama City Minato Red Cross Hospital, Kanagawa, Japan.

Objectives: The diagnostic utility of En1 in the histopathologic differentiation of eccrine porocarcinoma (EPC) from invasive squamous cell carcinoma (SCC) was investigated.

Methods: Expression of En1 and CK19 in 16 cases of EPC was immunohistochemically examined and compared with that in 32 cases of SCC.

Results: In all 16 EPCs, En1 was expressed in 3% to 100% of tumor cells. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa066DOI Listing

Effects of Implementing the Dual Papanicolaou Test Interpretation of ASC-H and LSIL Following Bethesda 2014.

Am J Clin Pathol 2020 Jun 18. Epub 2020 Jun 18.

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine-New York Presbyterian Hospital, New York, NY.

Objectives: To evaluate the impact of implementing the dual interpretation of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and low-grade squamous intraepithelial lesion (LSIL) after the Bethesda System 2014 and to compare it with other indeterminate interpretations.

Methods: Rates of high-risk human papillomavirus (HPV) positivity and histologic follow-up and the proportion of women with high-grade squamous intraepithelial lesion on histologic follow-up were compared for the combined interpretation of ASC-H and LSIL (ASCHL) and the categories of LSIL, cannot exclude high-grade squamous intraepithelial lesion (LSIL-H) and ASC-H.

Results: The percentage of ASCHL HPV-positive cases (86. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa069DOI Listing
June 2020
2.514 Impact Factor

Frontline Workers in the Backrooms of COVID-19.

Am J Clin Pathol 2020 Jun 17. Epub 2020 Jun 17.

American Society for Clinical Pathology, Chicago, IL.

Objectives: To review the response to the coronavirus disease 2019 (COVID-19) pandemic in a forensics center that integrates an academic department of pathology with multiple regional county medical examiners' offices.

Methods: Faculty and staff were asked to volunteer stories, data, and photographs describing their activities from March through May 2020. The information was assembled into a narrative summary. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa106DOI Listing

Placental Pathology in COVID-19.

Am J Clin Pathol 2020 Jun 13. Epub 2020 Jun 13.

Northwestern University Chicago, IL.

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http://dx.doi.org/10.1093/ajcp/aqaa101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314265PMC

Suspicious for Malignancy Diagnoses on Pleural Effusion Cytology.

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

Department of Pathology, Division of Cytopathology, Johns Hopkins Hospital, Baltimore, MD.

Objectives: A definitive diagnosis of malignancy may not be possible in pleural effusions. We report our experience with the diagnosis of suspicious for malignancy (SFM) in pleural effusion.

Methods: A search for pleural effusions diagnosed as SFM (2008-2018) was performed. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa058DOI Listing

CALIPER Hematology Reference Standards (II).

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Canada.

Objectives: The objective of this study was to establish comprehensive age- and sex-specific reference intervals for hematologic parameters in the CALIPER cohort of healthy children and adolescents.

Methods: A total of 536 healthy children and adolescents (birth to 21 years) were recruited with informed consent, and whole blood samples were analyzed for 27 hematologic parameters on the Beckman Coulter DxH 520 system. Age- and sex-specific pediatric reference standards were established. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa057DOI Listing

Contemporary Classification and Diagnostic Evaluation of Hypereosinophilia.

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

Departments of Pathology and Laboratory Medicine, University of California, San Francisco, San Francisco.

Objectives: To provide an in-depth review of the classification and diagnostic evaluation of hypereosinophilia (HE), with a focus on eosinophilic neoplasms.

Methods: A review of published literature was performed, and exemplary HE cases were identified.

Results: Causes of HE are diverse and can be grouped under three categories: primary (neoplastic), secondary (reactive), and idiopathic. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa056DOI Listing

Real-Time Intraoperative Consultation Reporting in the Electronic Health Record.

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.

Objectives: We sought to make pathologists' intraoperative consultation (IOC) results immediately available to the surgical team, other clinicians, and laboratory medicine colleagues to improve communication and decrease postanalytic errors.

Methods: We created an IOC report in our stand-alone laboratory information system that could be signed out prior to, and independent of, the final report, and transfer immediately to the electronic health record (EHR) as a preliminary diagnosis. We evaluated two metrics: preliminary (IOC) result review in the EHR by clinicians and postanalytic errors. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa050DOI Listing

Comparison of Tissue Molecular Biomarker Testing Turnaround Times and Concordance Between Standard of Care and the Biocartis Idylla Platform in Patients With Colorectal Cancer.

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

Carolina Blood and Cancer Care Associates, PA, Rock Hill, SC.

Objectives: Management of colorectal cancer warrants mutational analysis of KRAS/NRAS when considering anti-epidermal growth factor receptor therapy and BRAF testing for prognostic stratification. In this multicenter study, we compared a fully integrated, cartridge-based system to standard-of-care assays used by participating laboratories.

Methods: Twenty laboratories enrolled 874 colorectal cancer cases between November 2017 and December 2018. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa044DOI Listing
June 2020
2.514 Impact Factor

A Reappraisal of Superficial Pleomorphic Liposarcoma.

Am J Clin Pathol 2020 Jun 11. Epub 2020 Jun 11.

Department of Pathology, Virginia Commonwealth University Health System, Richmond.

Objectives: Superficial pleomorphic liposarcoma (PL) has a favorable prognosis compared to deeply seated PL. Given developments in the classification of lipomatous neoplasms, we reappraised a series of cases.

Methods: Retrospective clinicopathologic evaluation and genome-wide single-nucleotide polymorphism (SNP) microarray studies were performed for cases previously designated superficial PL. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa045DOI Listing

Linking Statistics With Testing Policy to Manage COVID-19 in the Community.

Am J Clin Pathol 2020 Jun 10. Epub 2020 Jun 10.

RAND Corporation, Santa Monica, CA.

Objectives: To determine the public health surveillance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing volume needed, both for acute infection and seroprevalence.

Methods: Required testing volumes were developed using standard statistical methods based on test analytical performance, disease prevalence, desired precision, and population size.

Results: Widespread testing for individual health management cannot address surveillance needs. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314266PMC

Clinical Evaluation and Utilization of Multiple Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2.

Am J Clin Pathol 2020 May 28. Epub 2020 May 28.

OhioHealth Laboratory Services, Columbus.

Objectives: To evaluate the clinical performance of 3 molecular assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Methods: We used 184 nasopharyngeal swab specimens to compare Abbott ID NOW COVID-19 (Abbott ID NOW), DiaSorin Molecular Simplexa COVID-19 Direct (DiaSorin Simplexa), and Roche cobas 6800 SARS-CoV-2 (Roche cobas) assays. In a separate analysis, 3 specimens (nasopharyngeal, oropharyngeal, and nasal) were collected from 182 unique patients presenting to the emergency department with suspicion of coronavirus disease 2019 and were tested utilizing Abbott ID NOW. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314271PMC

The Uterine Plexiform Lesions Revisited.

Am J Clin Pathol 2020 May 27. Epub 2020 May 27.

Department of Pathology, University Hospital of Saint-Etienne, France.

Objectives: Uterine lesions with plexiform morphology are uncommon lesions with debated histogenesis. Despite being an incidental and usually benign finding (plexiform tumorlet), some cases can pose diagnostic problems. Their paucity in the recent literature adds to these difficulties and often causes ambiguities. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa054DOI Listing

Predominant Stroma-Rich Feature in Hyaline Vascular Variant of Castleman Disease Is Associated With Paraneoplastic Pemphigus.

Am J Clin Pathol 2020 May 27. Epub 2020 May 27.

Department of Dermatology, Peking University First Hospital, Beijing, China.

Objectives: We aimed to describe the clinical and histopathologic features of Castleman disease (CD), particularly emphasizing its associations with paraneoplastic pemphigus (PNP) and prognosis.

Methods: We retrospectively enrolled 123 CD patients at our center. Clinical, pathologic, and laboratory data were reviewed. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa053DOI Listing

Four-Year Experience of Digital Slide Telepathology for Intraoperative Frozen Section Consultations in a Two-Site French Academic Department of Pathology.

Am J Clin Pathol 2020 May 27. Epub 2020 May 27.

Department of Pathology, AP-HP-Université Paris Saclay, Hôpital Bicêtre, Le Kremlin-Bicêtre, France.

Objectives: To share our experience with digital slide telepathology for intraoperative frozen section consultations (IOCs) and to describe its evolution over time by reporting performance metrics and addressing organizational and economic aspects.

Methods: Since 2013, a technician has been alone at the surgical site. At the other site, the pathologist opens the digital slide from a local server via the intranet. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa055DOI Listing

How I Diagnose Low-Grade Myelodysplastic Syndromes.

Am J Clin Pathol 2020 Jun;154(1):5-14

Department of Pathology, Massachusetts General Hospital, Boston.

Objectives: Myelodysplastic syndromes (MDS) are a group of myeloid neoplasms that are often difficult to diagnose due to their pathologic and clinical heterogeneity. The key features of MDS are peripheral blood cytopenias, ineffective hematopoiesis manifesting as morphologic dysplasia, and clonal genetic abnormalities. The most difficult diagnostic dilemmas often arise in low-grade MDS cases (lacking excess blasts), which can be difficult to distinguish from other causes of cytopenia. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa046DOI Listing

How I Diagnose.

Am J Clin Pathol 2020 Jun;154(1)

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis.

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http://dx.doi.org/10.1093/ajcp/aqaa034DOI Listing

Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases.

Am J Clin Pathol 2020 May 26. Epub 2020 May 26.

Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.

Objectives: To report methods and findings of 2 autopsies with molecular evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive individuals.

Methods: Postmortem examination was completed following Centers for Disease Control and Prevention public guidelines. Numerous formalin-fixed paraffin-embedded (FFPE) tissue types from each case were surveyed for SARS-CoV-2 RNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Read More

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http://dx.doi.org/10.1093/ajcp/aqaa091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314275PMC

Is Merkel Cell Carcinoma of Lymph Node Actually Metastatic Cutaneous Merkel Cell Carcinoma?

Am J Clin Pathol 2020 May 23. Epub 2020 May 23.

Department of Pathology, Stanford University School of Medicine, Stanford, CA.

Objectives: The possibility of a so-called primary lymph node neuroendocrine carcinoma has been described in the literature. Here we evaluate cases fitting such a diagnosis and find that the cases demonstrate a convincing and pervasive pattern consistent with metastatic Merkel cell carcinoma.

Methods: Six cases of primary lymph node Merkel cell carcinoma and one case of metastatic neuroendocrine carcinoma at a bony site, all with unknown primary, were sequenced using a combination of whole-exome and targeted panel methods. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa051DOI Listing

Serology Is More Sensitive Than Urea Breath Test or Stool Antigen for the Initial Diagnosis of Helicobacter pylori Gastritis When Compared With Histopathology.

Am J Clin Pathol 2020 May 23. Epub 2020 May 23.

Department of Laboratory Medicine, University of Washington, Seattle.

Objectives: To assess the concordance and performance characteristics of Helicobacter pylori laboratory tests compared with histopathology and to propose algorithms for the diagnosis of H pylori that minimize diagnostic error.

Methods: H pylori diagnostics were reviewed from a 12-year period within a health system (2,560 cases). Analyses were performed to adjust diagnostic performance based on treatment and consensus histopathologic diagnoses among pathologists. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa043DOI Listing

Cryoprecipitate Utilization Patterns Observed With a Required Prospective Approval Process vs Electronic Dosing Guidance.

Am J Clin Pathol 2020 May 23. Epub 2020 May 23.

Division of Transfusion Medicine, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD.

Objectives: We evaluated the impact of electronic medical record (EMR)-guided pooled cryoprecipitate dosing vs our previous practice of requiring transfusion medicine (TM) resident approval for every cryoprecipitate transfusion.

Methods: At our hospital, cryoprecipitate pooled from five donors is dosed for adult patients, while single-donor cryoprecipitate is dosed for pediatric patients (defined as patients <50 kg in weight). EMR-based dosing guidance replaced a previously required TM consultation when cryoprecipitate pools were ordered, but a consultation remained required for single-unit orders. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa042DOI Listing

Molecular/Cytogenetic Education for Hematopathology Fellows.

Am J Clin Pathol 2020 May 23. Epub 2020 May 23.

Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora.

Objectives: At a discussion on molecular/cytogenetic education for hematopathology fellows at the 2018 Society for Hematopathology Program Directors Meeting, consensus was that fellows should understand basic principles and indications for and limitations of molecular/cytogenetic testing used in routine practice. Fellows should also be adept at integrating results of such testing for rendering a final diagnosis. To aid these consensus goals, representatives from the Society for Hematopathology and the Association for Molecular Pathology formed a working group to devise a molecular/cytogenetic curriculum for hematopathology fellow education. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa038DOI Listing

Placental Pathology in COVID-19.

Am J Clin Pathol 2020 06;154(1):23-32

Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Objectives: To describe histopathologic findings in the placentas of women with coronavirus disease 2019 (COVID-19) during pregnancy.

Methods: Pregnant women with COVID-19 delivering between March 18, 2020, and May 5, 2020, were identified. Placentas were examined and compared to historical controls and women with placental evaluation for a history of melanoma. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279066PMC

Antibody Testing for COVID-19.

Am J Clin Pathol 2020 06;154(1):1-3

Case Western Reserve University School of Dental Medicine, Cleveland, OH.

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http://dx.doi.org/10.1093/ajcp/aqaa082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239247PMC

Reporting of Benign Endometrial Cells in Papanicolaou Tests.

Am J Clin Pathol 2020 May 14. Epub 2020 May 14.

Department of Pathology , NYU Langone Health, New York, NY.

Objectives: The 2014 Bethesda System (TBS 2014) guidelines for reporting cervical cytology revised the age for reporting benign endometrial cells (BECs) from 40 years or older to age 45 years or older. We evaluated this change and further investigated if extending the reporting age to 50 years or older may be acceptable.

Methods: We reviewed cases with BECs reported on Papanicolaou tests in women age 40 years or older and 45 years or older before and after implementation of TBS 2014. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa041DOI Listing

Evaluating Percentage-Based Reporting of Glucose-6-Phosphate Dehydrogenase (G6PD) Enzymatic Activity.

Am J Clin Pathol 2020 May 14. Epub 2020 May 14.

Department of Pathology, University of Utah, Salt Lake City.

Objectives: The World Health Organization recommends measurement of glucose-6-phosphate dehydrogenase (G6PD) activity before initiation of 8-aminoquinoline therapy. A new drug for malaria prophylaxis and treatment (tafenoquine) is contraindicated in patients with G6PD deficiency or unknown G6PD status given its prolonged half-life. Assessments of percentage of normal G6PD activity using laboratory-specific result distributions are not widely available, making tafenoquine-eligibility decisions potentially challenging. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa040DOI Listing

Corrigendum.

Authors:

Am J Clin Pathol 2020 May;153(6):852

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http://dx.doi.org/10.1093/ajcp/aqaa070DOI Listing

Histology-Independent Signature Distinguishes Kikuchi-Fujimoto Disease/Systemic Lupus Erythematosus-Associated Lymphadenitis From Benign and Malignant Lymphadenopathies.

Am J Clin Pathol 2020 May 5. Epub 2020 May 5.

Department of Pathology, Stanford University School of Medicine, Stanford, CA.

Objectives: Kikuchi-Fujimoto disease (KFD) and systemic lupus erythematosus (SLE) are benign entities with histologic features that raise concern about malignancy and infection. We searched for a histology-independent KFD/SLE signature relying on only immunophenotype and basic clinical characteristics.

Methods: A histology-independent KFD/SLE signature was generated using 975 excised lymph nodes with flow immunophenotyping, including 16 cases of KFD/SLE. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa036DOI Listing

Detection of Falsely Elevated Point-of-Care Potassium Results Due to Hemolysis Using Predictive Analytics.

Am J Clin Pathol 2020 May 5. Epub 2020 May 5.

Department of Pathology, NorthShore University HealthSystem, Evanston, IL.

Objectives: Preanalytical factors, such as hemolysis, affect many components of a test panel. Machine learning can be used to recognize these patterns, alerting clinicians and laboratories to potentially erroneous results. In particular, machine learning might identify which cases of elevated potassium from a point-of-care (POC) basic metabolic panel are likely erroneous. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa039DOI Listing

cDNA-Based Mutation Screening Using a Combination of High-Resolution Melting Curve and Fragment Analysis Facilitates Efficient CCR4 Mutation Analysis in Adult T-Cell Leukemia/Lymphoma.

Am J Clin Pathol 2020 May 5. Epub 2020 May 5.

Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan.

Objectives: C-C chemokine receptor type 4 (CCR4) proteins are expressed on the neoplastic cells of adult T-cell leukemia/lymphoma (ATLL). As the mutation status of CCR4 gene is reported to correlate with significant clinical information such as prognosis and response to mogamulizumab, we aimed to establish a screening method that is suitable for clinical laboratory tests.

Methods: In 34 patients with ATLL, CCR4 mutation analysis, high-resolution melting (HRM) analysis, fragment analysis, and direct sequencing were performed using both genomic DNA and complementary DNA (cDNA). Read More

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http://dx.doi.org/10.1093/ajcp/aqaa037DOI Listing

The Burden of Placental Histopathology in Stillbirths Associated With Maternal Obesity.

Am J Clin Pathol 2020 Apr 27. Epub 2020 Apr 27.

Department of Health Sciences, San Paolo Hospital Medical School, University of Milan, Milan, Italy.

Objectives: Obesity is an increasing health problem that has become a common medical disorder among women of childbearing age, representing worldwide a risk factor for stillbirth. The aim of the study is to evaluate the association between placental histopathologic findings and obesity in stillbirth.

Methods: Placentas were analyzed according to the Amsterdam consensus statement. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa035DOI Listing

Double-Edged Spike.

Am J Clin Pathol 2020 May;153(6):709-711

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT.

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http://dx.doi.org/10.1093/ajcp/aqaa071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188132PMC

Amplification of MDM2 and Loss of p16 Expression: Do They Have a Role in Malignant Transformation of Ovarian Brenner Tumor?

Am J Clin Pathol 2020 Jun;154(1):133-141

Department of Pathology, NYU Langone Medical Center, New York, NY.

Objectives: To review the significance of MDM2 and cyclin D1 expression and loss of p16 expression in malignant and borderline Brenner tumors (BTs) of the ovary.

Methods: We describe 2 new cases of ovarian BT, 1 malignant and 1 borderline. We studied MDM2, p16, and cyclin D1 expression by immunohistochemistry in the benign, borderline, and malignant components of these 2 cases and in 5 additional cases of benign BT. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa033DOI Listing