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    17247 results match your criteria American journal of clinical pathology[Journal]

    1 OF 345

    Philadelphia Chromosome-like Mixed-Phenotype Acute Leukemia Demonstrating P2RY8-CRLF2 Fusion and JAK1 Mutation.
    Am J Clin Pathol 2017 Nov 11. Epub 2017 Nov 11.
    Departments of Pathology.
    Objectives: Philadelphia chromosome-like (Ph-like) genetic alterations define a subset of B lymphoblastic leukemia/lymphoma (B-ALL), which represents a separate provisional entity in the World Health Organization 2016 updated classification. However, these alterations have not been described outside the context of B-ALL.

    Methods: Cytogenomic array and molecular analysis identified a Ph-like signature in a mixed-phenotype acute leukemia (MPAL), B/myeloid, confirmed using conventional immunophenotypic and cytochemical analysis. Read More

    The Apoptotic Crypt Abscess: An Underappreciated Histologic Finding in Gastrointestinal Pathology.
    Am J Clin Pathol 2017 Nov 11. Epub 2017 Nov 11.
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
    Objectives: To differentiate apoptotic crypt abscesses (ACAs) from neutrophilic crypt abscesses (NCAs).

    Methods: Cases with crypt abscesses were classified as containing ACAs, NCAs, or mixed crypt abscesses (MCAs) by H&E staining. Sections were stained with cleaved caspase 3 and myeloperoxidase and recategorized. Read More

    Evaluation of Opportunities to Improve Hematopathology Diagnosis for Vietnam Pathologists.
    Am J Clin Pathol 2017 Nov 11. Epub 2017 Nov 11.
    Center for Global Health, American Society for Clinical Pathology.
    Objectives: We evaluate the need for, feasibility of, and impediments to improving hematopathology diagnoses for cancer hospitals in Vietnam.

    Methods: Two hematopathologists from the United States visited three major cancer treatment hospitals in Vietnam to workshop a sampling of difficult hematopathology cases. With Vietnamese pathologists, they toured histopathology, immunohistochemistry, and ancillary laboratory facilities. Read More

    KLHL6 Is Preferentially Expressed in Germinal Center-Derived B-Cell Lymphomas.
    Am J Clin Pathol 2017 Nov 11. Epub 2017 Nov 11.
    Department of Pathology.
    Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells.

    Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Read More

    CD30 Expression in Monomorphic Posttransplant Lymphoproliferative Disorder, Diffuse Large B-Cell Lymphoma Correlates With Greater Regulatory T-Cell Infiltration.
    Am J Clin Pathol 2017 Nov 8. Epub 2017 Nov 8.
    Department of Pathology, Medical College of Wisconsin, Milwaukee.
    Objectives: CD30 is a protein thought to promote cell proliferation/survival and downregulate the immune response. Twenty percent to 40% of de novo diffuse large B-cell lymphomas (DLBCLs) express CD30, and some patients have been treated with the anti-CD30 agent brentuximab. In the solid organ transplant setting, allograft regulatory T cells (Tregs) have been shown to be modulated via CD30 signaling. Read More

    Gastric Carcinomas With Lymphoid Stroma: Categorization and Comparison With Solid-Type Colonic Carcinomas.
    Am J Clin Pathol 2017 Nov 8. Epub 2017 Nov 8.
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.
    Objectives: To determine whether histologic features could help identify gastric carcinomas with lymphoid stroma associated with microsatellite instability (MSI) (ie, "medullary carcinomas"), Epstein-Barr virus (EBV) infection (termed lymphoepithelioma-like carcinomas in other organ systems), or neither.

    Methods: We identified 17 solid-type gastric carcinomas with lymphoid stroma, assessed EBV and MSI status, and compared features across groups. We also compared them with 51 solid-type colorectal adenocarcinomas. Read More

    Epitope Preservation Methods for Tissue Microarrays: Longitudinal Prospective Study.
    Am J Clin Pathol 2017 Nov;148(5):380-389
    Department of Pathology, University of Washington, Seattle.
    Objectives: We sought to test recent guidelines for preserving immunoreactivity of precut slides, to quantify loss of immunoreactivity, and to determine potential for preservation by altering storage conditions.

    Methods: Precut slides from tissue microarrays were stored under one of several conditions: exposed to ambient air at room temperature, 4°C, or -20°C or in a vacuum-sealed container at room temperature, -20°C, -80°C, or with paraffin coating. At multiple intervals over 1 year, slides were stained with antibodies against p53, isocitrate dehydrogenase 1, Ki-67, synaptophysin, and androgen receptor and evaluated. Read More

    Rapid Loss of RNA Detection by In Situ Hybridization in Stored Tissue Blocks and Preservation by Cold Storage of Unstained Slides.
    Am J Clin Pathol 2017 Nov;148(5):398-415
    Department of Pathology.
    Objectives: Recent commercialization of methods for in situ hybridization using Z-pair probe/branched DNA amplification has led to increasing adoption of this technology for interrogating RNA expression in formalin-fixed, paraffin-embedded (FFPE) tissues. Current practice for FFPE block storage is to maintain them at room temperature, often for many years.

    Methods: To examine the effects of block storage time on FFPE tissues using a number of RNA in situ probes with the Advanced Cellular Diagnostic's RNAscope assay. Read More

    Submucosal Invasive Depth Predicts Lymph Node Metastasis and Poor Prognosis in Submucosal Invasive Esophageal Squamous Cell Carcinoma.
    Am J Clin Pathol 2017 Nov;148(5):416-426
    Division of Pathology, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
    Objectives: Lymph node metastasis (LNM) in submucosal invasive esophageal squamous cell carcinoma (SM-ESCC) is a prognostic factor. The aim of this study was to identify a histopathologic predictor of LNM in SM-ESCC.

    Methods: In total, 108 patients who underwent an esophagectomy and lymph node dissection without preoperative therapy and who were pathologically diagnosed with SM-ESCC were enrolled in this study. Read More

    Undifferentiated Pancreatic Carcinomas Display Enrichment for Frequency and Extent of PD-L1 Expression by Tumor Cells.
    Am J Clin Pathol 2017 Nov;148(5):441-449
    Department of Laboratory Medicine and Pathology.
    Objectives: Programmed death ligand 1 (PD-L1) expression in pancreatic ductal adenocarcinoma (PDA) has been described, but unselected PDAs have shown limited clinical responsiveness to anti-programmed death 1 (PD-1)/PD-L1 therapy.

    Methods: We studied 24 cases of undifferentiated pancreatic carcinoma (UPC) using immunohistochemistry for PD-L1 (E1L3N clone), CD3, CD20, CD68, and DNA mismatch repair proteins in this study. Slides were scored for extent of PD-L1 expression on tumor cells and tumor-infiltrating immune cells. Read More

    Evaluation of a Reflex Testing Algorithm for Suspected Heparin-Induced Thrombocytopenia.
    Am J Clin Pathol 2017 Nov;148(5):390-397
    Departments of Pathology.
    Objectives: We implemented a policy of reflex serotonin-release assay (SRA) testing for all patients with a positive heparin-induced thrombocytopenia (HIT) immunoassay.

    Methods: We identified all patients who had SRA testing sent as a consequence of a positive HIT immunoassay test. We reviewed charts of patients to calculate the 4Ts clinical score, determined the effect of testing on clinical management, and documented the change in utilization of direct thrombin inhibitors (DTIs). Read More

    Performance of the cobas HPV Test for the Triage of Atypical Squamous Cells of Undetermined Significance Cytology in Cervical Specimens Collected in SurePath.
    Am J Clin Pathol 2017 Nov;148(5):450-457
    Roche Molecular Systems, Pleasanton, CA.
    Objectives: Determine performance of the cobas human papillomavirus (HPV) test for triage of atypical squamous cells of undetermined significance (ASC-US) in SurePath.

    Methods: Women presenting for routine screening had cervical specimens collected in SurePath and specimen transport medium (STM); those with ASC-US cytology underwent colposcopy. Performance of cobas HPV in SurePath specimens that had undergone a preanalytic procedure to reverse possible cross-linking of HPV DNA was compared with Hybrid Capture 2 (hc2) specimens in STM. Read More

    Drone Transport of Chemistry and Hematology Samples Over Long Distances.
    Am J Clin Pathol 2017 Nov;148(5):427-435
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD.
    Objectives: We addressed the stability of biological samples in prolonged drone flights by obtaining paired chemistry and hematology samples from 21 adult volunteers in a single phlebotomy event-84 samples total.

    Methods: Half of the samples were held stationary, while the other samples were flown for 3 hours (258 km) in a custom active cooling box mounted on the drone. After the flight, 19 chemistry and hematology tests were performed. Read More

    Use of the Mean Abnormal Result Rate (MARR) to Gauge Changes in Family Physicians' Selectivity of Laboratory Test Ordering, 2010-2015.
    Am J Clin Pathol 2017 Nov;148(5):436-440
    Calgary Laboratory Services, Calgary, Canada.
    Objectives: The mean abnormal result rate (MARR) has recently been advanced as a metric of laboratory test appropriateness. We used the MARR metric to examine patterns of change in family physician test requisitions over time.

    Methods: We accessed the Laboratory Information System of Calgary Laboratory Services for family physician-ordered testing on outpatients to gather aggregate test and abnormal result counts from 2010 to 2015. Read More

    Minimal Clinical Impact of Intraoperative Examination of Sentinel Lymph Nodes in Patients With Ductal Carcinoma In Situ: An Opportunity for Improved Resource Utilization.
    Am J Clin Pathol 2017 Nov;148(5):374-379
    Departments of Pathology.
    Objectives: There is little information regarding sentinel lymph node (SLN) frozen-section examination in patients with a history of ductal carcinoma in situ (DCIS). We evaluated the usage, clinical impact, and pathology resources used for SLN cryosectioning in mastectomy cases with a DCIS history.

    Methods: Mastectomies with SLNs submitted from 2012 to 2013 at a tertiary care center were analyzed. Read More

    Variability and Error in Cardiac Troponin Testing: An ACLPS Critical Review.
    Am J Clin Pathol 2017 Oct;148(4):281-295
    Department of Laboratory Medicine, University of Washington, Seattle.
    Objectives: To provide a comprehensive overview of the complexities associated with cardiac troponin (cTn) testing. An emphasis is placed on the sources of error, organized into the preanalytical, analytical, and postanalytical phases of the testing pathway. Controversial areas are also explored. Read More

    Intraoperative Pathologic Consultation on Hysterectomy Specimens for Endometrial Cancer: An Assessment of the Accuracy of Frozen Sections, "Gross-Only" Evaluations, and Obtaining Random Sections of a Grossly "Normal" Endometrium.
    Am J Clin Pathol 2017 Oct;148(4):345-353
    Department of Pathology,University of California, San Diego.
    Objectives: Pathologic intraoperative consultation (IOC) is a common approach for segregating the subset of patients with endometrial cancer who likely require a lymphadenectomy.

    Methods: We evaluate factors related to the performance and value of IOC, including the accuracy of frozen sections, "gross-only examinations," and obtaining random sections when a gross lesion is not apparent.

    Results: IOC was performed by gross examination only in 17 (8%) of 250 cases, the specificity and negative predictive value of which in diagnosing cancer were 100% and 85%, respectively. Read More

    Deviation Management: Key Management Subsystem Driver of Knowledge-Based Continuous Improvement in the Henry Ford Production System.
    Am J Clin Pathol 2017 Oct;148(4):354-367
    Pathology and Laboratory Medicine, Henry Ford Health System, Detroit, MI.
    Objectives: To develop a business subsystem fulfilling International Organization for Standardization 15189 nonconformance management regulatory standard, facilitating employee engagement in problem identification and resolution to effect quality improvement and risk mitigation.

    Methods: From 2012 to 2016, the integrated laboratories of the Henry Ford Health System used a quality technical team to develop and improve a management subsystem designed to identify, track, trend, and summarize nonconformances based on frequency, risk, and root cause for elimination at the level of the work.

    Results: Programmatic improvements and training resulted in markedly increased documentation culminating in 71,641 deviations in 2016 classified by a taxonomy of 281 defect types into preanalytic (74. Read More

    Ensuring the Quality of Point-of-Care Testing in a Large and Decentralized Ambulatory Care Setting.
    Am J Clin Pathol 2017 Oct;148(4):336-344
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA.
    Objectives: In this project, we assessed the breadth, quality, trends, and outcomes of point-of-care (POC) testing and regulatory compliance in 200 University of California, Los Angeles (UCLA) Health system outpatient clinics.

    Methods: We retrospectively extracted clinic POC test orders, results, and e-prescription data from the UCLA electronic health record over a 1-year period.

    Results: Over 100,000 total tests were performed, encompassing 10 POC tests. Read More

    Real-Time Methylation-Specific Polymerase Chain Reaction for MGMT Promoter Methylation Clinical Testing in Glioblastoma: An Alternative Detection Method for a Heterogeneous Process.
    Am J Clin Pathol 2017 Oct;148(4):296-307
    Departments of Laboratory Medicine and Pathology.
    Objectives: To develop and evaluate a real-time methylation-specific polymerase chain reaction (RT-MSP) MGMT assay, with a particular focus on small biopsies and indeterminate testing results.

    Methods: We assessed formalin-fixed paraffin-embedded glioblastoma or gliosarcoma specimens (n = 641). A test-validation group (n = 51) with previously obtained reference laboratory (RL) results was used to determine performance characteristics of the RT-MSP assay. Read More

    Impact of 2013 American Society of Clinical Oncology/College of American Pathologists Guidelines on HER2 Fluorescent In Situ Hybridization Testing in Breast Cancers : Experience From a National Reference Laboratory.
    Am J Clin Pathol 2017 Oct;148(4):308-313
    Department of Pathology, University of Utah, Huntsman Cancer Hospital, Salt Lake City.
    Objectives: We compared the impact of 2013 American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guidelines on human epidermal growth factor receptor 2 (HER2) fluorescence in situ hybridization (FISH) testing results on breast cancers.

    Methods: HER2 FISH testing performed between May 2015 and April 2016 following 2013 ASCO/CAP guidelines was included. HER2 to control probe ratios, mean HER2, and control probe copy numbers were used to reassign HER2 status using 2007 ASCO/CAP and US Food and Drug Administration (FDA) guidelines. Read More

    Impact of Use of Smaller Volume, Smaller Vacuum Blood Collection Tubes on Hemolysis in Emergency Department Blood Samples.
    Am J Clin Pathol 2017 Oct;148(4):330-335
    Cleveland Clinic Nursing Institute, Cleveland Clinic, Cleveland, OH.
    Objectives: Hemolyzed blood samples commonly occur in hospital emergency departments (EDs). Our objective was to determine whether replacing standard large-volume/high-vacuum sample tubes with low-volume/low-vacuum tubes would significantly affect ED hemolysis.

    Methods: This was a prospective intervention of the use of small-volume/vacuum collection tubes. Read More

    Utility of Reference Change Values for Delta Check Limits.
    Am J Clin Pathol 2017 Oct;148(4):323-329
    Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, Korea.
    Objectives: To assess the utility of reference change values (RCVs) as delta check limits.

    Methods: A total of 1,650,518 paired results for 23 general chemistry test results from June 1, 2014, to October 31, 2016, were analyzed. The RCVs for each analyte were calculated from the analytical imprecision and within-subject biological variation. Read More

    A Survey of the Prevalence and Impact of Reporting Guideline Endorsement in Pathology Journals.
    Am J Clin Pathol 2017 Oct;148(4):314-322
    Department of Pathology and ARUP Laboratories, University of Utah Health Sciences Center, Salt Lake City.
    Objectives: To determine the prevalence of reporting guideline endorsement in pathology journals and to estimate the impact of guideline endorsement.

    Methods: We compared the quality of reporting in two sets of studies: (1) studies published in journals that explicitly mentioned a guideline vs studies published in journals that did not and (2) studies that cited a guideline vs studies that did not. The quality of reporting in prognostic biomarker studies was assessed using the REporting recommendations for tumor MARKer prognostic studies (REMARK) guideline. Read More

    Creation and Evaluation of a Laboratory Administration Curriculum for Pathology Residents.
    Am J Clin Pathol 2017 Oct;148(4):368-373
    Department of Pathology and Laboratory Medicine, Johns Hopkins University, Baltimore, MD.
    Objectives: A clinical laboratory management (CLM) curriculum that can objectively assess the Accreditation Council for Graduate Medical Education pathology systems-based practice milestones and can provide consistent resident training across institutions is needed.

    Methods: Faculty at Emory University created a curriculum that consists of assay verification exercises and interactive, case-based online modules. Beta testing was done at Emory University and Johns Hopkins. Read More

    Hyalinizing Clear Cell Carcinoma of the Lung: Case Report and Review of the Literature.
    Am J Clin Pathol 2017 Jul;148(1):73-80
    Division of Hematology and Oncology, University of Arkansas for Medical Sciences, Little Rock.
    Objectives: Hyalinizing clear cell carcinoma (HCCC) is common in head and neck sites but extremely rare in the lung. This case report describes an HCCC in the lung of a 54-year-old female patient.

    Methods: We summarize the histomorphologic, immunophenotypic, and molecular features for our and three previously reported HCCCs of the lung with emphasis on potential diagnostic pitfalls. Read More

    Frequency and Clinicopathologic Features of RUNX1 Mutations in Patients With Acute Myeloid Leukemia Not Otherwise Specified.
    Am J Clin Pathol 2017 Jul;148(1):64-72
    Departments of Laboratory Medicine.
    Objectives: To evaluate the frequency and clinicopathologic characteristics of RUNX1 mutations, focusing on patients with acute myeloid leukemia not otherwise specified (AML NOS).

    Methods: Diagnostic samples from 219 patients with AML NOS were analyzed for RUNX1 mutations using standard polymerase chain reaction and direct sequencing.

    Results: Thirty-one RUNX1 mutations were detected in 33 (15. Read More

    Myelodysplastic Syndrome, Unclassifiable (MDS-U) With 1% Blasts Is a Distinct Subgroup of MDS-U With a Poor Prognosis.
    Am J Clin Pathol 2017 Jul;148(1):49-57
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College/New York Presbyterian Hospital, New York, NY.
    Objectives: Three situations qualify as myelodysplastic syndrome, unclassifiable (MDS-U): (1) refractory cytopenia with dysplasia and 1% blasts in peripheral blood (BL), (2) pancytopenia with unilineage dysplasia (Pan), and (3) persistent cytopenia, less than 5% bone marrow blasts, and less than 10% dysplastic cells and presence of MDS-defining cytogenetic abnormalities (CG). We compared the clinicopathologic features and mutational profiles for these three groups.

    Methods: MDS-U cases were reviewed at four major academic institutions. Read More

    Defining Papillary Carcinoma of the Thyroid: A Short Review and Analysis.
    Am J Clin Pathol 2017 Aug;148(2):100-107
    VA and Duke University Medical Centers, Durham, NC.
    Objectives: To review how changes in the pathologic definitions for papillary tumors of the thyroid during recent decades have affected outcomes for patients with these tumors.

    Methods: Forty-nine previous reports or studies involving collectively 53,606 patients were reviewed, and new analyses were performed on the data to include analyses of agreement, incidence, survival, and diagnostic categories.

    Results: The past emphasis on cytologic features to define papillary tumors has not resulted in ideal pairwise agreement between pathologists and has produced incidence and survival data suggesting overdetection and overdiagnosis. Read More

    Real-Time Clinical Decision Support Decreases Inappropriate Plasma Transfusion.
    Am J Clin Pathol 2017 Aug;148(2):154-160
    Departments of Pathology.
    Objectives: To curtail inappropriate plasma transfusions, we instituted clinical decision support as an alert upon order entry if the patient's recent international normalized ratio (INR) was 1.7 or less.

    Methods: The alert was suppressed for massive transfusion and within operative or apheresis settings. Read More

    Role of Histone H3K27 Trimethylation Loss as a Marker for Malignant Peripheral Nerve Sheath Tumor in Fine-Needle Aspiration and Small Biopsy Specimens.
    Am J Clin Pathol 2017 Aug;148(2):179-189
    Department of Pathology, Brigham and Women's Hospital, Boston, MA.
    Objectives: Accurate diagnosis of malignant peripheral nerve sheath tumor (MPNST) is often challenging on fine-needle aspiration (FNA) or core needle biopsy. Recurrent mutations in EED and SUZ12, which encode subunits of polycomb repressive complex 2 (PRC2), have been identified in 70% to 92% of MPNSTs; PRC2 inactivation leads to loss of trimethylation of lysine 27 of histone H3 (H3K27me3). We evaluated the utility of H3K27me3 immunohistochemistry for distinguishing MPNST from its cytomorphologic mimics. Read More

    Comparison of Oncotype DX With Modified Magee Equation Recurrence Scores in Low-Grade Invasive Carcinoma of Breast.
    Am J Clin Pathol 2017 Aug;148(2):167-172
    Department of Pathology, Wexner Medical Center at The Ohio State University, Columbus.
    Objectives: Several specific histologic types of invasive carcinoma of breast, including invasive tubular carcinoma (ITC), invasive mucinous carcinoma (IMC), and classical invasive lobular carcinoma (CILC), are considered low-grade carcinomas with favorable outcomes. We aimed to investigate the utility of Oncotype DX test in these tumors by comparing its recurrence score (RS) with the modified Magee equation RS.

    Methods: Oncotype DX RSs were collected and modified Magee equation RSs were calculated in 163 low-grade invasive breast carcinomas, including 105 CILCs, 41 ITCs, and 17 IMCs. Read More

    Typical and Atypical Granular Cell Tumors of Soft Tissue: A Clinicopathologic Study of 50 Patients.
    Am J Clin Pathol 2017 Aug;148(2):161-166
    Department of Pathology, Medical College of Wisconsin, Milwaukee.
    Objectives: Granular cell tumors are rare neoplasms of neural origin. Despite the mesenchymal nature of these tumors, they rarely occur in the soft tissue, and as a result, this subset is not well characterized. We present the largest case series to date comprising 50 patients with benign and atypical soft tissue granular cell tumors in an effort to better define the pathologic features in this subset of lesions. Read More

    Building a New Transfusion Service.
    Am J Clin Pathol 2017 Aug;148(2):173-178
    Department of Laboratory Medicine, University of Washington School of Medicine, Seattle.
    Objectives: For over 60 years, Harborview Medical Center (HMC) in Seattle has received its blood components and pretransfusion testing from a centralized transfusion service operated by the regional blood supplier. In 2011, a hospital-based transfusion service (HBTS) was activated.

    Methods: After 5 years of operation, we evaluated the effects of the HBTS by reviewing records of hospital blood use, quality system events, blood product delivery times, and costs. Read More

    Lineage Switch Between B-Lymphoblastic Leukemia and Acute Myeloid Leukemia Intermediated by "Occult" Myelodysplastic Neoplasm: Two Cases of Adult Patients With Evidence of Genomic Instability and Clonal Selection by Chemotherapy.
    Am J Clin Pathol 2017 Aug;148(2):136-147
    Department of Pathology, Duke University Medical Center, Durham, NC.
    Objectives: Lineage switch occurs in rare leukemias, and the mechanism is unclear. We report two cases of B-lymphoblastic leukemia (B-ALL) relapsed as acute myeloid leukemia (AML).

    Methods: Retrospective review of clinical and laboratory data. Read More

    Analysis of Daily Laboratory Orders at a Large Urban Academic Center: A Multifaceted Approach to Changing Test Ordering Patterns.
    Am J Clin Pathol 2017 Aug;148(2):128-135
    Departments of Pathology.
    Objectives: We sought to address concerns regarding recurring inpatient laboratory test order practices (daily laboratory tests) through a multifaceted approach to changing ordering patterns.

    Methods: We engaged in an interdepartmental collaboration to foster mindful test ordering through clinical policy creation, electronic clinical decision support, and continuous auditing and feedback.

    Results: Annualized daily order volumes decreased from approximately 25,000 to 10,000 during a 33-month postintervention review. Read More

    Ki-67 Expression in Breast Cancer Tissue Microarrays: Assessing Tumor Heterogeneity, Concordance With Full Section, and Scoring Methods.
    Am J Clin Pathol 2017 Aug;148(2):108-118
    Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY.
    Objectives: Ki-67 has been proposed to be used as a surrogate marker to differentiate luminal breast carcinomas (BCs). The purpose of this study was to determine the utility of and best approaches for using tissue microarrays (TMAs) and Ki-67 staining to distinguish luminal subtypes in large epidemiology studies of luminal/human epidermal growth factor receptor 2 (HER2)-negative BC.

    Methods: Full-section and TMA (three 0. Read More

    Prevalence of Anal Human Papillomavirus (HPV) and Performance of Cepheid Xpert and Hybrid Capture 2 (hc2) HPV Assays in South African HIV-Infected Women.
    Am J Clin Pathol 2017 Aug;148(2):148-153
    Institute of Infectious Disease and Molecular Medicine and Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
    Objectives: This study investigated anal high-risk HPV (HR-HPV) prevalence in HIV-infected women using the Cepheid Xpert HPV assay and compares its performance with that of Hybrid Capture-2 (hc2).

    Methods: A total of 199 HIV-infected women were recruited from Helen Joseph Hospital, Johannesburg. Stored ThinPrep anal swabs that had previously been tested using hc2 were tested for HPV using Xpert. Read More

    Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus From Nasal Swabs.
    Am J Clin Pathol 2017 Aug;148(2):119-127
    Medical and Scientific Affairs, Roche Molecular Diagnostics, Pleasanton, CA.
    Objectives: Health care-associated methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (SA) infections are continuing problems. Rapidly determining the MRSA colonization status of a patient facilitates practice to reduce spread of MRSA clinical disease. Sensitive detection of all SA prior to surgery, followed by decolonization, can significantly reduce postoperative infection from this pathogen. Read More

    Keratin 17 Is a Prognostic Biomarker in Endocervical Glandular Neoplasia.
    Am J Clin Pathol 2017 Sep;148(3):264-273
    Departments of Pathology.
    Objectives: Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). K17, however, has not been previously evaluated in endocervical glandular neoplasia. Based on the similar pathogenesis of squamous and glandular lesions of the cervix, we hypothesized that K17 overexpression could also be a diagnostic and/or prognostic biomarker for endocervical neoplasia. Read More

    CD200 Expression in Neuroendocrine Neoplasms.
    Am J Clin Pathol 2017 Sep;148(3):236-242
    PhenoPath Laboratories, Seattle, WA.
    Objectives: CD200 expression has been well studied in hematopoietic malignancies; however, CD200 expression has not been well-characterized in neuroendocrine neoplasms. We examined CD200 expression in 391 neuroendocrine neoplasms from various anatomic sites.

    Methods: Tissue blocks containing pulmonary small cell carcinoma, pulmonary carcinoid, large cell neuroendocrine carcinoma, pancreatic neuroendocrine tumor, gastrointestinal carcinoid, and Merkel cell carcinoma were evaluated for CD200 expression by immunohistochemistry. Read More

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