17,448 results match your criteria American journal of clinical pathology[Journal]


PD-L1 Expression Reveals Significant Association With Squamous Differentiation in Upper Tract Urothelial Carcinoma.

Am J Clin Pathol 2019 Feb 18. Epub 2019 Feb 18.

Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia.

Objectives: Limited literature is available on the tumor microenvironment (TM) of upper tract urothelial carcinoma (UTUC). This study comprehensively reviews programmed death 1 receptor (PD-1)-positive and CD8+ tumor-infiltrating lymphocytes (TILs) and programmed death ligand 1 (PD-L1) expression on tumor epithelium (TE).

Methods: Seventy-two nephroureterectomy specimens were analyzed for PD-L1, PD-1, and CD8. Read More

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http://dx.doi.org/10.1093/ajcp/aqz002DOI Listing
February 2019

Isolated Flat Epithelial Atypia on Core Biopsy Specimens Is Associated With a Low Risk of Upgrade at Excision.

Am J Clin Pathol 2019 Feb 11. Epub 2019 Feb 11.

University of Pittsburgh Medical Center, Pittsburgh, PA.

Objectives: We present a retrospective review of 111 breast core biopsy specimens with flat epithelial atypia (FEA) and corresponding excision to better understand rates of upgrade following this diagnosis.

Methods: In total, 252 breast core biopsy specimens were identified. Cases were excluded if biopsy slides or excision results were unavailable, for ipsilateral carcinoma or other findings warranting excision, or biopsy performed for indications other than mammographically detected calcifications. Read More

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http://dx.doi.org/10.1093/ajcp/aqy175DOI Listing
February 2019

Correction.

Authors:

Am J Clin Pathol 2019 Feb;151(3):349

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https://academic.oup.com/ajcp/article/151/3/349/5306290
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http://dx.doi.org/10.1093/ajcp/aqy177DOI Listing
February 2019
4 Reads

Detection of Nicotine and Nicotine Metabolites in Units of Banked Blood.

Am J Clin Pathol 2019 Feb 3. Epub 2019 Feb 3.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: To determine the concentrations of nicotine and nicotine metabolites in RBC units as a means to estimate the point prevalence of exposure within the healthy donor pool.

Methods: Segments from 105 RBC units were tested for the presence of nicotine, cotinine, or trans-3'-hydroxycotinine by liquid chromatography-tandem mass spectrometry.

Results: Of the 20 (19%) units that contained detectable concentrations of nicotine, cotinine, or trans-3'-hydroxycotinine, 19 (18. Read More

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http://dx.doi.org/10.1093/ajcp/aqy176DOI Listing
February 2019

Susceptibility of Cardiac Troponin Assays to Biotin Interference.

Am J Clin Pathol 2019 Feb 2. Epub 2019 Feb 2.

Department of Pathology, Clements University Hospital, University of Texas Southwestern Medical Center, Dallas.

Objectives: To investigate biotin interference on three cardiac troponin (cTn) assays and demonstrate a method to overcome biotin interference.

Methods: cTn levels were measured in (1) plasma from healthy volunteers on 10-mg daily biotin supplementation mixed with a plasma with known elevated troponin, (2) plasmas with known elevated cTn after mixing in reagent biotin to simulate supplementation, and (3) biotin-spiked plasma specimens pretreated with streptavidin-agarose beads.

Results: Daily biotin ingestion (10 mg) and studies simulating daily biotin use resulted in significant interference in the Gen5 cardiac troponin T (cTnT) assay; the contemporary Gen 4 cTnT and high-sensitivity cardiac troponin I (hs-cTnI) assays were unaffected. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy172DOI Listing
February 2019
6 Reads

Utility of a Simple and Robust Flow Cytometry Assay for Rapid Clonality Testing in Mature Peripheral T-Cell Lymphomas.

Am J Clin Pathol 2019 Jan 31. Epub 2019 Jan 31.

Department of Pathology, Brigham and Women's Hospital, Boston, MA.

Objectives: Flow cytometry immunophenotyping is limited by poor resolution of T-cell clones. A newly described antibody was recently used to distinguish normal peripheral blood T cells from malignant T-cell clones. Here, we evaluate this antibody as a new diagnostic tool for detecting T-cell clonality in mature peripheral T-cell lymphomas. Read More

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http://dx.doi.org/10.1093/ajcp/aqy173DOI Listing
January 2019

Immunohistochemical Nuclear Expression of β-Catenin as a Surrogate of CTNNB1 Exon 3 Mutation in Endometrial Cancer.

Am J Clin Pathol 2019 Feb 2. Epub 2019 Feb 2.

Gynecology and Obstetrics Unit, Department of Neuroscience, Reproductive Sciences and Dentistry, School of Medicine.

Objectives: CTNNB1 exon 3 mutations have shown independent prognostic value in endometrial cancer. We aimed to assess whether nuclear β-catenin expression is an accurate surrogate, as immunohistochemistry is cheaper, faster, and more widely applicable than sequencing.

Methods: A systematic review was performed by searching electronic databases for all studies assessing the association between β-catenin immunohistochemical expression and CTNNB1 mutations. Read More

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http://dx.doi.org/10.1093/ajcp/aqy178DOI Listing
February 2019
2.514 Impact Factor

Time-Series Analysis of Laboratory Values in the Context of Long-Term Hospitalized Patient Mortality.

Am J Clin Pathol 2019 Jan 28. Epub 2019 Jan 28.

Department of Pathology, State University of New York Downstate Medical Center, Brooklyn.

Objectives: Long-term hospitalized patients have a higher risk of adverse outcomes and mortality rate. These patients often rapidly deteriorate, leading to death. We aim to evaluate end-of-life laboratory values time trends among deceased long-term inpatients. Read More

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http://dx.doi.org/10.1093/ajcp/aqy163DOI Listing
January 2019
1 Read

The FDA-Approved Breast Cancer HER2 Evaluation Kit (HercepTest; Dako) May Miss Some HER2-Positive Breast Cancers.

Am J Clin Pathol 2019 Jan 21. Epub 2019 Jan 21.

Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA.

Objectives: Accurate evaluation of human epidermal growth factor receptor 2 (HER2) in breast cancer is critical.

Methods: HER2 fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) tests were performed on 52 cases using a US Food and Drug Administration (FDA)-approved kit (HercepTest, FDA kit) and a laboratory-developed test (LDT) with the HercepTest antibody and a Leica Bond automated stainer.

Results: By FISH, 22 were HER2 positive, 29 were negative, and one was equivocal. Read More

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http://dx.doi.org/10.1093/ajcp/aqy171DOI Listing
January 2019
1 Read

Radiofrequency Interference in the Clinical Laboratory: Case Report and Review of the Literature.

Am J Clin Pathol 2019 Jan 21. Epub 2019 Jan 21.

Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, OH.

Objectives: Radiofrequency interference (RFI) is a known medical device safety issue, but there are no documented cases of interference resulting in erroneous laboratory results.

Methods: We investigated unexpected failure of a hematology analyzer resulting in erroneous WBC counts. Hardware failure was initially suspected, but temporal association with increased power output from a nearby antenna prompted investigation for RFI. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy174DOI Listing
January 2019
2 Reads

Diagnostic Utility of UroVysion Combined With Conventional Urinary Cytology for Urothelial Carcinoma of the Upper Urinary Tract.

Am J Clin Pathol 2019 Jan 21. Epub 2019 Jan 21.

Department of Surgical Pathology, Aichi Medical University Hospital, Nagakute, Japan.

Objectives: We prospectively evaluated the utility of UroVysion in urothelial carcinomas of the upper urinary tract (UCUUTs).

Methods: Ninety patients who received nephroureterectomy for UCUUT were enrolled. We performed urinary cytology and UroVysion before nephroureterectomy. Read More

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http://dx.doi.org/10.1093/ajcp/aqy170DOI Listing
January 2019

HPV Testing With 16, 18, and 45 Genotyping Stratifies Cancer Risk for Women With Normal Cytology: Data From the Baseline Phase of the Onclarity Trial.

Am J Clin Pathol 2019 Jan 14. Epub 2019 Jan 14.

Becton, Dickinson and Company, BD Life Sciences-Diagnostic Systems, Sparks, MD.

Objectives: To determine the BD Onclarity human papillomavirus (HPV) assay performance and risk values for cervical intraepithelial neoplasia grade 2 (CIN2) or higher and cervical intraepithelial neoplasia grade 3 (CIN3) or higher during Papanicolaou/HPV cotesting in a negative for intraepithelial lesions or malignancies (NILM) population.

Methods: In total, 22,383 of the 33,858 enrolled women were 30 years or older with NILM cytology. HPV+ and a subset of HPV- patients (3,219/33,858 combined; 9. Read More

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http://dx.doi.org/10.1093/ajcp/aqy169DOI Listing
January 2019
2 Reads

Impact of Specimen Type and Specimen Number on HER2 Status in Gastroesophageal Junction and Gastric Adenocarcinoma: More Is Better.

Am J Clin Pathol 2019 Jan 8. Epub 2019 Jan 8.

Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY.

Objectives: Human epidermal growth factor receptor 2 (HER2) is expressed in some gastric and gastroesophageal junction adenocarcinomas. There were two goals: assess the impact of specimen type on HER2 status and evaluate HER2 concordance with multiple specimens.

Methods: All cases were evaluated by immunohistochemistry (IHC) for HER2 and interpreted using established criteria. Read More

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http://dx.doi.org/10.1093/ajcp/aqy166DOI Listing
January 2019
1 Read

Correction.

Authors:

Am J Clin Pathol 2019 Jan;151(2):240

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https://academic.oup.com/ajcp/article/151/2/240/5261363
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http://dx.doi.org/10.1093/ajcp/aqy159DOI Listing
January 2019
1 Read

Rete Testis Invasion Is Consistent With Pathologic Stage T1 in Germ Cell Tumors.

Am J Clin Pathol 2018 Dec 20. Epub 2018 Dec 20.

Medical College of Wisconsin, Milwaukee.

Objectives: Rete testis invasion by germ cell tumors is frequently concomitant with lymphovascular or spermatic cord invasion (LVI/SCI); independent implications for staging are uncertain.

Methods: In total, 171 seminomas and 178 nonseminomatous germ cell tumors (NSGCTs; 46 had 1%-60% seminoma component) came from five institutions. Metastatic status at presentation, as a proxy for severity, was available for all; relapse data were unavailable for 152. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/ajcp/aqy168DOI Listing
December 2018
2 Reads

Application of Fluorescence In Situ Hybridization on Cerebrospinal Fluid Cytospins for the Detection of Residual Leukemic Cells in Patients With Childhood Acute Lymphoblastic Leukemia.

Am J Clin Pathol 2018 Dec 17. Epub 2018 Dec 17.

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test.

Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Read More

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http://dx.doi.org/10.1093/ajcp/aqy160DOI Listing
December 2018
2 Reads

Three Approaches to Creating an Essential Diagnostics List.

Am J Clin Pathol 2018 Dec 15. Epub 2018 Dec 15.

Department of Pathology and Laboratory Medicine, Johns Hopkins University, Baltimore, MD.

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy167DOI Listing
December 2018
9 Reads

Two Extraordinary Sellar Neuronal Tumors: Literature Review and Comparison of Clinicopathologic Features.

Am J Clin Pathol 2019 Feb;151(3):241-254

Department of Pathology, Duke University Medical Center, Durham, NC.

Objectives: The list of tumors involving the pituitary gland has been expanded to include a variety of neuronal and paraneuronal tumors in the 2017 World Health Organization tumor classification of endocrine organs. All the entities included in this category are distinctly rare, with limited case reports in the literature.

Methods: We illustrate two extraordinary sellar tumors with neuronal differentiation: a sellar paraganglioma and a sellar neurocytoma, with thorough literature review and comparison of the clinicopathologic features of these entities. Read More

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http://dx.doi.org/10.1093/ajcp/aqy155DOI Listing
February 2019
3 Reads

Establishing Commonsense-Based Statistical Quality Control Practices.

Am J Clin Pathol 2018 Dec 5. Epub 2018 Dec 5.

Service of Laboratory Medicine, Hospital General de Requena, Valencia, Spain.

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http://dx.doi.org/10.1093/ajcp/aqy162DOI Listing
December 2018
1 Read

Quality Improvement in Transfusion Practice of Orthotopic Liver Transplantation Reduces Blood Utilization, Length of Hospital Stay, and Cost.

Am J Clin Pathol 2018 Dec 8. Epub 2018 Dec 8.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: Orthotopic liver transplantation (OLT) can require substantial usage of blood products. Higher rates of transfusion have been associated with increased length of hospital stay, higher rates of infection, graft failure, and mortality. This study was a retrospective analysis to assess the impact of quality improvement interventions in OLT. Read More

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http://dx.doi.org/10.1093/ajcp/aqy154DOI Listing
December 2018
1 Read

The Top 25 Laboratory Tests by Volume and Revenue in Five Different Countries.

Am J Clin Pathol 2018 Dec 6. Epub 2018 Dec 6.

Pathology and Laboratory Services, Denver Health Medical Center, Denver, CO.

Objectives: To compare the most common diagnostic/laboratory tests across five different referral hospitals by volume and revenue.

Methods: The authors obtained data on volumes and reimbursement rates for the most common 25 tests at the five hospitals with which they are affiliated and organized them to be as comparable as possible. Simple descriptive statistics were used to make cross-country comparisons. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy165DOI Listing
December 2018
10 Reads
2.514 Impact Factor

Assessment of Programmed Death-Ligand 1 (PD-L1) Immunohistochemical Expression on Cytology Specimens in Non-Small Cell Lung Carcinoma: A Comparative Study With Paired Surgical Specimens.

Am J Clin Pathol 2018 Dec 6. Epub 2018 Dec 6.

NYU Langone Medical Center, New York, NY.

Objectives: To evaluate whether non-small cell lung carcinoma (NSCLC) cytology specimens are reliable for programmed death-ligand 1 (PD-L1) immunohistochemical (IHC) testing.

Methods: Fifty-two cell blocks (CBs) with corresponding surgical pathology PD-L1 IHC testing were stained with a Dako PD-L1 pharmDX antibody (clone-22C3). Tumor cellularity was recorded as <100 or ≥100 cells. Read More

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http://dx.doi.org/10.1093/ajcp/aqy164DOI Listing
December 2018
1 Read

Diagnostic Utility of Isolated Tube C Positivity in T-Cell Receptor β Testing Using BIOMED-2 Primers.

Am J Clin Pathol 2018 Dec 8. Epub 2018 Dec 8.

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA.

Objectives: T-cell receptor (TCR) gene rearrangement studies are widely used for assessing T-cell clonality. The frequency and significance of clonal peaks restricted to TCR β (TCRB) tube C are uncertain. We retrospectively reviewed 80 TCR studies performed on bone marrow/peripheral blood. Read More

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http://dx.doi.org/10.1093/ajcp/aqy157DOI Listing
December 2018
1 Read

Establishing Evidence-Based Statistical Quality Control Practices.

Am J Clin Pathol 2018 Dec 5. Epub 2018 Dec 5.

Westgard QC, Madison, WI.

Objectives: To establish an objective, scientific, evidence-based process for planning statistical quality control (SQC) procedures based on quality required for a test, precision and bias observed for a measurement procedure, probabilities of error detection and false rejection for different control rules and numbers of control measurements, and frequency of QC events (or run size) to minimize patient risk.

Methods: A Sigma-Metric Run Size Nomogram and Power Function Graphs have been used to guide the selection of control rules, numbers of control measurements, and frequency of QC events (or patient run size).

Results: A tabular summary is provided by a Sigma-Metric Run Size Matrix, with a graphical summary of Westgard Sigma Rules with Run Sizes. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy158DOI Listing
December 2018
2 Reads

Discordant Partial Thromboplastin Time (PTT) vs Anti-Xa Heparin Activity: Using the Baseline PTT to Improve Concordance.

Am J Clin Pathol 2018 Nov 25. Epub 2018 Nov 25.

Department of Laboratory Medicine, University of Washington, Seattle.

Objectives: To determine the relationship between baseline variations in the partial thromboplastin time (PTT) and the discordance between the PTT and anti-Xa heparin activity (anti-Xa) during heparin therapy.

Methods: The baseline PTT on heparin was determined using automated heparin neutralization with protamine (prPTT). The prPTT was used to calculate a baseline-corrected PTT on heparin to reduce discordance with anti-Xa measurements. Read More

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http://dx.doi.org/10.1093/ajcp/aqy156DOI Listing
November 2018
1 Read

Widespread Incorrect Implementation of the Hoffmann Method, the Correct Approach, and Modern Alternatives.

Am J Clin Pathol 2019 Feb;151(3):328-336

Biostatistics and Medical Informatics, University of Wisconsin, Madison.

Objectives: The Hoffmann method is a procedure for reference interval estimation using routine clinical results. Many authors incorrectly prepare Hoffmann plots on a linear rather than normal probability scale. We explore the consequences. Read More

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http://dx.doi.org/10.1093/ajcp/aqy149DOI Listing
February 2019
1 Read

Comparison of Phenotypic Activated Protein C Resistance Testing With a Genetic Assay for Factor V Leiden.

Am J Clin Pathol 2019 Feb;151(3):302-305

Department of Laboratory Medicine, Division of Clinical Coagulation, University of Washington, Seattle.

Objectives: To compare the accuracy and reliability of phenotypic activated protein C resistance (aPC-R) assays with a genotypic assay for the factor V Leiden F5 p.R506Q (FVL) mutation.

Methods: Data were obtained from an electronic data warehouse for FVL testing performed at an academic institution with a large referral laboratory service. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy142DOI Listing
February 2019
3 Reads

Analytical and Clinical Performance Evaluation of the Elecsys HIV combi PT Assay on the cobas e 602 Analyzer for the Diagnosis of Human Immunodeficiency Virus.

Am J Clin Pathol 2018 Nov 13. Epub 2018 Nov 13.

Baylor Scott & White Health, Temple, TX.

Objectives: We evaluated the performance of the Elecsys HIV combi PT assay on the cobas e 602 analyzer for diagnosing human immunodeficiency virus (HIV; part of the US Food and Drug Administration [FDA] submission).

Methods: The HIV combi PT and reference (ARCHITECT HIV Ag/Ab Combo) assays were assessed at four independent clinical laboratories/one reference laboratory (United States; July 2014 to November 2015). Clinical performance was evaluated using four reagent lots. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy153DOI Listing
November 2018
29 Reads

Cost per Case Mix Index-Adjusted Hospital Day as a Measure of Effective Laboratory Utilization Efforts in a Growing Academic Medical Center.

Am J Clin Pathol 2018 Nov 13. Epub 2018 Nov 13.

Department of Pathology, Cooper University Hospital, Camden, NJ.

Objectives: Traditional laboratory utilization measures are unable to detect the results of small-scale utilization improvement efforts in a background of rising patient volumes and acuity. However, accurate assessment is necessary to document effectiveness of these efforts.

Methods: Test menu changes, physician education, and laboratory utilization feedback were used to address costs and overused tests. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy152DOI Listing
November 2018
13 Reads

Mild Allergic Transfusion Reactions: Impact of Associated Clinical Symptoms?

Am J Clin Pathol 2019 Feb;151(3):344-348

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: Transfusions are often needlessly aborted after occurrence of a mild allergic transfusion reaction (ATR), leading to wastage and reexposure of recipients to additional blood products (with potential alloimmunization). We aimed to determine the symptoms associated with such reactions (along with other parameters) as a possible reason of concern for transfusionists aborting such transfusions.

Methods: We reviewed the symptomology of all mild ATRs (as well as the associated wastage and costs of aborted transfusions) at an academic medical center that occurred over a period of 1 year. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy150DOI Listing
February 2019
9 Reads

Breast Cancers With Magee Equation Score of Less Than 18, or 18-25 and Mitosis Score of 1, Do Not Require Oncotype DX Testing: A Value Study.

Am J Clin Pathol 2019 Feb;151(3):316-323

Department of Pathology, Magee-Womens Hospital, University of Pittsburgh Medical Center, Pittsburgh, PA.

Objectives: To investigate use of Magee equations (MEs) to determine which breast cancer cases can be excluded from Oncotype DX testing.

Methods: A prospective value study was carried out using data from pathology reports.

Results: If all three MEs scores were less than 18 or 31 or higher, the cases were labeled do not send for testing. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360636PMC
February 2019
1 Read

Laboratory Monitoring of Platelet P2Y12 Receptor Inhibitors and Reversal of Antiplatelet Agents: An ACLPS Critical Review.

Am J Clin Pathol 2018 Nov 3. Epub 2018 Nov 3.

Stony Brook University Medical Center, Stony Brook, NY.

Objectives: To review the use of laboratory tests for antiplatelet agents to determine escalation of antiplatelet therapy and for emergent reversal of P2Y12 inhibitors.

Methods: A case scenario and review of cardiovascular and neurointerventional literature are described.

Results: In cardiovascular disease patients, large randomized trials failed to demonstrate superiority of tailored antiplatelet regimens using the VerifyNow P2Y12 assay, where earlier studies had shown promise. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy151DOI Listing
November 2018
9 Reads

Reference Values for WBC Differential by Hematoflow Analysis.

Am J Clin Pathol 2019 Feb;151(3):324-327

Laboratoire Hématologie, Centre Hospitalier Universitaire Rennes, Rennes, France.

Objectives: WBC differentials performed using flow cytometry with monoclonal antibodies have been developed in the last decade and are nowadays integrated into the routine workflow of some laboratories. Definition of reference values for each population is required in order to achieve an automatic validation of the results by laboratory software.

Methods: We analyzed 584 samples from three hospitals using the Hematoflow solution to define the reference values. Read More

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http://dx.doi.org/10.1093/ajcp/aqy147DOI Listing
February 2019
2 Reads

Microarray-Based Nucleic Acid Assay and MALDI-TOF MS Analysis for the Detection of Gram-Negative Bacteria in Direct Blood Cultures.

Am J Clin Pathol 2019 Jan;151(2):143-153

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.

Objectives: To compare a microarray-based identification and resistance determination system (blood culture gram-negative [BC-GN]; Nanosphere, Northbrook, IL) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for direct blood cultures (BCs).

Methods: BC-GN and MALDI-TOF MS assay results from direct BCs were compared with conventional test results after pure culture.

Results: Among 124 BCs, 130 gram-negative rods (GNRs), including six cultures with mixed GNRs (117 bacteria were covered by the BC-GN panel), were detected. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy118DOI Listing
January 2019
5 Reads

Comparison of the Seventh and Eighth Editions of the American Joint Committee on Cancer pT and pN Classifications as Predictors of Survival in Patients With Oral Squamous Cell Carcinoma.

Am J Clin Pathol 2019 Feb;151(3):292-301

Department of Clinical Oncology, Delhi State Cancer Institute, Delhi, India.

Objectives: We retrospectively compared the seventh and eighth editions of American Joint Committee on Cancer (AJCC) TNM frameworks as disease-free survival (DFS) and overall survival (OS) predictors in oral squamous carcinomas (OSCCs).

Methods: We restaged the 342 patients with the revised pT and pN criteria and performed survival analyses.

Results: The 3-year DFS (mean follow-up, 364 days; recurrences, 99) was 50%, and the 5-year OS (mean follow-up, 615 days; deaths, 69) was 42%. Read More

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http://dx.doi.org/10.1093/ajcp/aqy143DOI Listing
February 2019
2 Reads

Optimization of Laboratory Ordering Practices for Complete Blood Count With Differential.

Am J Clin Pathol 2019 Feb;151(3):306-315

Departments of Pathology, University of Alabama at Birmingham.

Objectives: To investigate the utilization of CBC and CBC with differential (CBC w/diff) tests at University of Alabama at Birmingham Hospital, and to determine if a reduction in CBC w/diff tests could be achieved without negatively impacting patient care.

Methods: The quantity of testing and distribution of repeated tests before, during, and after an educational intervention were compared.

Results: CBC w/diff tests were ordered 10-fold more frequently than CBC tests. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360635PMC
February 2019
9 Reads
2.514 Impact Factor

Portal Cavernoma Cholangiopathy: Histologic Features and Differential Diagnosis.

Am J Clin Pathol 2019 Feb;151(3):255-262

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY.

Objectives: Portal cavernoma cholangiopathy (formerly portal biliopathy) is a type of biliary injury that occurs in association with a portal vein thrombus or cavernoma. Although the radiographic features of portal cavernoma cholangiopathy have been enumerated in the literature, its histologic features have not been described in detail.

Methods: We describe the histologic findings in liver specimens from three patients with radiologically confirmed portal cavernoma cholangiopathy. Read More

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http://dx.doi.org/10.1093/ajcp/aqy132DOI Listing
February 2019
4 Reads

Anemia Management and Audit Feedback Practices for Reducing Overuse of RBC Transfusion: A Laboratory Medicine Best Practice Systematic Review and Meta-Analysis.

Am J Clin Pathol 2019 Jan;151(1):18-28

Gauss Surgical, Los Altos, CA.

Objectives: To evaluate the effectiveness of anemia management and audit with feedback practices in reducing overuse of RBC transfusion.

Methods: This review follows the Centers for Disease Control and Prevention's Laboratory Medicine Best Practice Systematic Review (A-6) method. We searched the literature and solicited unpublished studies on practices to reduce overuse of RBC transfusions as measured by reductions in units transfused and proportion of patients transfused. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy123DOI Listing
January 2019
10 Reads

IRTA1 and MNDA Expression in Marginal Zone Lymphoma: Utility in Differential Diagnosis and Implications for Classification.

Am J Clin Pathol 2019 Feb;151(3):337-343

Department of Laboratory Medicine, Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH.

Objectives: To evaluate the clinical utility of immune receptor translocation-associated protein 1 (IRTA1) and myeloid nuclear differentiation antigen (MNDA) expression in the diagnosis and classification of marginal zone lymphomas (MZLs).

Methods: IRTA1 was examined using a novel RNA in situ hybridization assay and MNDA expression determined by immunohistochemistry in 127 small B-cell neoplasms, including 80 cases of MZL.

Results: IRTA1 expression was detected in 31 (42%) of 74 MZLs vs one (2%) of 43 other small B-cell neoplasms (P < . Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy144DOI Listing
February 2019
12 Reads
2.514 Impact Factor

Association of PD-L1, PD-L2, and Immune Response Markers in Matched Renal Clear Cell Carcinoma Primary and Metastatic Tissue Specimens.

Am J Clin Pathol 2019 Jan;151(2):217-225

The Cleveland Clinic Taussig Cancer Institute, Cleveland, OH.

Objectives: Immune checkpoint therapy has been promising in renal cell carcinoma, but no validated clinically relevant biomarkers exist. Metastatic deposits may have discordant biomarker expression.

Methods: Fifty matched pairs of primary and metastatic kidney tumors were evaluated via immunohistochemistry for immune checkpoint proteins PD-1, PD-L1, and PD-L2 and the T-cell and macrophage surface markers CD3, FOXP3, and CD163. Read More

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http://dx.doi.org/10.1093/ajcp/aqy141DOI Listing
January 2019
4 Reads

Measuring Scientific Impact With the h-Index: A Primer for Pathologists.

Am J Clin Pathol 2019 Feb;151(3):286-291

Biomedical Branch Library, University of British Columbia, Vancouver, Canada.

Objectives: To describe the h-index as a measure of scientific output.

Methods: The formula for this index is based on (1) the number of papers published by an author and (2) the number of citations per paper. The h-index can be used to evaluate performance by individuals or by groups of scientists working in university departments or research institutes. Read More

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http://dx.doi.org/10.1093/ajcp/aqy137DOI Listing
February 2019
2 Reads

Identification of HER2 Immunohistochemistry-Negative, FISH-Amplified Breast Cancers and Their Response to Anti-HER2 Neoadjuvant Chemotherapy.

Am J Clin Pathol 2019 Jan;151(2):176-184

Department of Pathology, Wexner Medical Center at The Ohio State University, Columbus, OH.

Objectives: Either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (HER2) status. Breast cancers (BCs) with HER2 IHC-negative (IHC-) and ISH-amplified (ISH+) results have been rarely reported but not well studied. We investigated the frequency of HER2 IHC-/ISH+ BCs and their response to anti-HER2 neoadjuvant chemotherapy (NAC). Read More

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http://dx.doi.org/10.1093/ajcp/aqy136DOI Listing
January 2019
1 Read

Evaluation of Peritumoral Fibrosis in Metastatic Colorectal Adenocarcinoma to the Liver Using Digital Image Analysis.

Am J Clin Pathol 2019 Jan;151(2):226-230

Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD.

Objectives: It is challenging to separate peritumoral fibrosis from fibrosis due to chronic liver disease in mass-directed liver biopsies. We evaluated the distance that peritumoral fibrosis extends from metastatic colorectal adenocarcinoma in liver.

Methods: Peritumoral and distant uninvolved liver trichrome stains from 25 cases were analyzed using digital image analysis. Read More

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http://dx.doi.org/10.1093/ajcp/aqy134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306046PMC
January 2019
1 Read

Implementation of Good Clinical Laboratory Practice in an Immunology Basic Research Laboratory: The KEMRI-Wellcome Trust Research Laboratories Experience.

Am J Clin Pathol 2019 Feb;151(3):270-274

Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, UK.

Objectives: Good Clinical Laboratory Practice (GCLP) is a standard that ensures quality and reliability of research data by adopting the principles of Good Laboratory Practice and Good Clinical Practice. Even though implementing a quality system in a basic research laboratory is still a contentious issue, it ensures that the research data are accurate, valid, and reliable. GCLP implementation requires proper documented procedures and safety precautions to achieve this objective. Read More

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http://dx.doi.org/10.1093/ajcp/aqy138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360634PMC
February 2019
4 Reads

Sensitivity of Cerebrospinal Fluid Cytology for the Diagnosis of Cryptococcal Infections: A 21-Year Single-Institution Retrospective Review.

Am J Clin Pathol 2019 Jan;151(2):198-204

Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH.

Objectives: Cryptococcal meningoencephalitis is the most common fungal infection of the central nervous system diagnosed by cerebrospinal fluid cytology (CSF) studies. Existing literature suggests that routine CSF cytomorphologic evaluations are exquisitely specific; however, less is known about their sensitivity.

Methods: An electronic record review of the cytopathology and microbiology files was conducted for the 21-year interval from January 1, 1995, through December 31, 2015. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy133DOI Listing
January 2019
3 Reads

Lymphoepithelioma-Like Carcinoma of the Uterine Cervix: A Pathologic Study of Eight Cases With Emphasis on the Association With Human Papillomavirus.

Am J Clin Pathol 2019 Jan;151(2):231-239

Department of Pathology, University of Miami Miller School of Medicine, Miami, FL.

Objectives: Lymphoepithelioma-like carcinoma (LELC) of the uterine cervix is a rare tumor. The goal of this study was to evaluate a series of cases of cervical LELC and to investigate possible association with human papillomavirus (HPV) and/or Epstein-Barr virus (EBV).

Methods: Immunohistochemistry for p63, p16, human leukocyte antigen-D related (HLA-DR), and B-cell lymphoma 2 (BCL-2); in situ hybridization (ISH) for EBV and HPV; and polymerase chain reaction (PCR) genotyping were performed. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy130DOI Listing
January 2019
12 Reads

False-Positive Light Chain Clonal Restriction by Flow Cytometry in Patients Treated With Alemtuzumab: Potential Pitfalls for the Misdiagnosis of B-Cell Neoplasms.

Am J Clin Pathol 2019 Jan;151(2):154-163

Department of Pathology, Yale School of Medicine, New Haven, CT.

Objectives: To increase awareness of potential diagnostic test interference associated with alemtuzumab, which is a therapeutic immunoglobulin G1 κ monoclonal antibody used in hematologic malignancies, autoimmune diseases, and transplant-related disorders.

Methods: Bone marrow and blood from patients with T-cell prolymphocytic leukemia treated with alemtuzumab were evaluated by flow cytometry. Healthy donor blood was analyzed with or without in vitro treatment with alemtuzumab for comparison. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy129DOI Listing
January 2019
3 Reads

Bone Marrow Biopsy Needle Type Affects Core Biopsy Specimen Length and Quality and Aspirate Hemodilution.

Am J Clin Pathol 2019 Jan;151(2):185-193

Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO.

Objectives: Bone marrow biopsies are essential for evaluating patients with suspected or confirmed hematopoietic disorders or malignancies, but little is known about how biopsy needle type affects biopsy length and/or quality. We sought to compare bone marrow biopsy quality in specimens obtained with two different needles.

Methods: A retrospective analysis was performed on bone marrow specimens obtained with manual single-bevel (n = 114) or triple-bevel (n = 166) needles. Read More

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http://dx.doi.org/10.1093/ajcp/aqy126DOI Listing
January 2019
6 Reads

Diagnostic Accuracy of β-d-Glucan (Fungitell) Testing Among Patients With Hematologic Malignancies or Solid Organ Tumors: A Systematic Review and Meta-Analysis.

Am J Clin Pathol 2019 Feb;151(3):275-285

Department of Pathology, Division of Infectious Disease, Health Sciences Center, University of Utah, Salt Lake City.

Objectives: To determine the accuracy of Fungitell, a β-d-glucan (BDG) test, for the diagnosis of invasive fungal infection (IFI) among cancer patients.

Methods: For this meta-analysis, MEDLINE and EMBASE were searched for references related to BDG testing. Study quality was evaluated using QUADAS-2. Read More

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http://dx.doi.org/10.1093/ajcp/aqy135DOI Listing
February 2019
2 Reads